LOCUS       NM_015571               6671 bp    mRNA    linear   PRI 01-AUG-2024
DEFINITION  Homo sapiens SUMO specific peptidase 6 (SENP6), transcript variant
            1, mRNA.
ACCESSION   NM_015571
VERSION     NM_015571.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6671)
  AUTHORS   Wang,Y.W., Jia,K.G., Xing,H.J., Pan,Y., Zeng,C.S., Chen,L.,
            Su,Q.J., Shen,W.T., Chen,J., Chen,C., Cao,Q. and Wang,Y.Y.
  TITLE     [Interaction of SENP6 with PINK1 Promotes Temozolomide Resistance
            in Neuroglioma Cells via Inducing the Mitophagy]
  JOURNAL   Mol Biol (Mosk) 58 (1), 126-129 (2024)
   PUBMED   38062972
  REMARK    GeneRIF: [Interaction of SENP6 with PINK1 Promotes Temozolomide
            Resistance in Neuroglioma Cells via Inducing the Mitophagy].
REFERENCE   2  (bases 1 to 6671)
  AUTHORS   He,Q., Chen,Z., Li,J., Liu,J., Zuo,Z., Lin,B., Song,K., Zhou,C.,
            Lai,H., Huang,Q. and Guo,X.
  TITLE     SENP6-Mediated deSUMOylation of VEGFR2 Enhances Its Cell Membrane
            Transport in Angiogenesis
  JOURNAL   Int J Mol Sci 24 (3), 2544 (2023)
   PUBMED   36768878
  REMARK    GeneRIF: SENP6-Mediated deSUMOylation of VEGFR2 Enhances Its Cell
            Membrane Transport in Angiogenesis.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6671)
  AUTHORS   Chen,K., Wang,Q., Liu,X., Wang,F., Ma,Y., Zhang,S., Shao,Z.,
            Yang,Y. and Tian,X.
  TITLE     Single Cell RNA-Seq Identifies Immune-Related Prognostic Model and
            Key Signature-SPP1 in Pancreatic Ductal Adenocarcinoma
  JOURNAL   Genes (Basel) 13 (10), 1760 (2022)
   PUBMED   36292645
  REMARK    GeneRIF: Single Cell RNA-Seq Identifies Immune-Related Prognostic
            Model and Key Signature-SPP1 in Pancreatic Ductal Adenocarcinoma.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6671)
  AUTHORS   Schick,M., Zhang,L., Maurer,S., Maurer,H.C., Isaakaidis,K.,
            Schneider,L., Patra,U., Schunck,K., Rohleder,E., Hofstetter,J.,
            Baluapuri,A., Scherger,A.K., Slotta-Huspenina,J., Hettler,F.,
            Weber,J., Engleitner,T., Maresch,R., Slawska,J., Lewis,R.,
            Istvanffy,R., Habringer,S., Steiger,K., Baiker,A.,
            Oostendorp,R.A.J., Miething,C., Lenhof,H.P., Bassermann,F.,
            Chapuy,B., Wirth,M., Wolf,E., Rad,R., Muller,S. and Keller,U.
  TITLE     Genetic alterations of the SUMO isopeptidase SENP6 drive
            lymphomagenesis and genetic instability in diffuse large B-cell
            lymphoma
  JOURNAL   Nat Commun 13 (1), 281 (2022)
   PUBMED   35022408
  REMARK    GeneRIF: Genetic alterations of the SUMO isopeptidase SENP6 drive
            lymphomagenesis and genetic instability in diffuse large B-cell
            lymphoma.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6671)
  AUTHORS   Mitra,S., Bodor,D.L., David,A.F., Abdul-Zani,I., Mata,J.F.,
            Neumann,B., Reither,S., Tischer,C. and Jansen,L.E.T.
  TITLE     Genetic screening identifies a SUMO protease dynamically
            maintaining centromeric chromatin
  JOURNAL   Nat Commun 11 (1), 501 (2020)
   PUBMED   31980633
  REMARK    GeneRIF: SENP6 activity is required throughout the cell cycle,
            suggesting that a dynamic SUMO cycle underlies a continuous
            surveillance of the centromere complex.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6671)
  AUTHORS   Bailey,D. and O'Hare,P.
  TITLE     Characterization of the localization and proteolytic activity of
            the SUMO-specific protease, SENP1
  JOURNAL   J Biol Chem 279 (1), 692-703 (2004)
   PUBMED   14563852
  REMARK    GeneRIF: SENP1 localization is influenced by expression and
            localization of SUMO-1-conjugated target proteins within the cell.
REFERENCE   7  (bases 1 to 6671)
  AUTHORS   Tojo,M., Matsuzaki,K., Minami,T., Honda,Y., Yasuda,H., Chiba,T.,
            Saya,H., Fujii-Kuriyama,Y. and Nakao,M.
  TITLE     The aryl hydrocarbon receptor nuclear transporter is modulated by
            the SUMO-1 conjugation system
  JOURNAL   J Biol Chem 277 (48), 46576-46585 (2002)
   PUBMED   12354770
REFERENCE   8  (bases 1 to 6671)
  AUTHORS   Goodson,M.L., Hong,Y., Rogers,R., Matunis,M.J., Park-Sarge,O.K. and
            Sarge,K.D.
  TITLE     Sumo-1 modification regulates the DNA binding activity of heat
            shock transcription factor 2, a promyelocytic leukemia nuclear body
            associated transcription factor
  JOURNAL   J Biol Chem 276 (21), 18513-18518 (2001)
   PUBMED   11278381
REFERENCE   9  (bases 1 to 6671)
  AUTHORS   Kim,K.I., Baek,S.H., Jeon,Y.J., Nishimori,S., Suzuki,T., Uchida,S.,
            Shimbara,N., Saitoh,H., Tanaka,K. and Chung,C.H.
  TITLE     A new SUMO-1-specific protease, SUSP1, that is highly expressed in
            reproductive organs
  JOURNAL   J Biol Chem 275 (19), 14102-14106 (2000)
   PUBMED   10799485
REFERENCE   10 (bases 1 to 6671)
  AUTHORS   Yeh,E.T., Gong,L. and Kamitani,T.
  TITLE     Ubiquitin-like proteins: new wines in new bottles
  JOURNAL   Gene 248 (1-2), 1-14 (2000)
   PUBMED   10806345
  REMARK    Review article
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL356057.12, CR990444.1, AF307849.1, AK021417.1 and AL109897.30.
            
            On Nov 22, 2018 this sequence version replaced NM_015571.3.
            
            Summary: Ubiquitin-like molecules (UBLs), such as SUMO1 (UBL1; MIM
            601912), are structurally related to ubiquitin (MIM 191339) and can
            be ligated to target proteins in a similar manner as ubiquitin.
            However, covalent attachment of UBLs does not result in degradation
            of the modified proteins. SUMO1 modification is implicated in the
            targeting of RANGAP1 (MIM 602362) to the nuclear pore complex, as
            well as in stabilization of I-kappa-B-alpha (NFKBIA; MIM 164008)
            from degradation by the 26S proteasome. Like ubiquitin, UBLs are
            synthesized as precursor proteins, with 1 or more amino acids
            following the C-terminal glycine-glycine residues of the mature UBL
            protein. Thus, the tail sequences of the UBL precursors need to be
            removed by UBL-specific proteases, such as SENP6, prior to their
            conjugation to target proteins (Kim et al., 2000 [PubMed
            10799485]). SENPs also display isopeptidase activity for
            deconjugation of SUMO-conjugated substrates (Lima and Reverter,
            2008 [PubMed 18799455]).[supplied by OMIM, Jun 2009].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660807.25575.1,
                                           SRR1660805.60699.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000447266.7/ ENSP00000402527.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-26                AL356057.12        123980-124005
            27-619              CR990444.1         1-593
            620-3771            AF307849.1         50-3201
            3772-4267           AK021417.1         1546-2041
            4268-6671           AL109897.30        3968-6371
FEATURES             Location/Qualifiers
     source          1..6671
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q14.1"
     gene            1..6671
                     /gene="SENP6"
                     /gene_synonym="SSP1; SUSP1"
                     /note="SUMO specific peptidase 6"
                     /db_xref="GeneID:26054"
                     /db_xref="HGNC:HGNC:20944"
                     /db_xref="MIM:605003"
     exon            1..697
                     /gene="SENP6"
                     /gene_synonym="SSP1; SUSP1"
                     /inference="alignment:Splign:2.1.0"
     CDS             646..3984
                     /gene="SENP6"
                     /gene_synonym="SSP1; SUSP1"
                     /EC_number="3.4.22.68"
                     /note="isoform 1 is encoded by transcript variant 1;
                     2810017C20Rik; SUMO1/sentrin specific protease 6;
                     sentrin-specific protease 6; SUMO-1-specific protease 1;
                     sentrin/SUMO-specific protease SENP6; SUMO1/sentrin
                     specific peptidase 6"
                     /codon_start=1
                     /product="sentrin-specific protease 6 isoform 1"
                     /protein_id="NP_056386.2"
                     /db_xref="CCDS:CCDS47454.1"
                     /db_xref="GeneID:26054"
                     /db_xref="HGNC:HGNC:20944"
                     /db_xref="MIM:605003"
                     /translation="MAAGKSGGSAGEITFLEALARSESKRDGGFKNNWSFDHEEESEG
                     DTDKDGTNLLSVDEDEDSETSKGKKLNRRSEIVANSSGEFILKTYVRRNKSESFKTLK
                     GNPIGLNMLSNNKKLSENTQNTSLCSGTVVHGRRFHHAHAQIPVVKTAAQSSLDRKER
                     KEYPPHVQKVEINPVRLSRLQGVERIMKKTEESESQVEPEIKRKVQQKRHCSTYQPTP
                     PLSPASKKCLTHLEDLQRNCRQAITLNESTGPLLRTSIHQNSGGQKSQNTGLTTKKFY
                     GNNVEKVPIDIIVNCDDSKHTYLQTNGKVILPGAKIPKITNLKERKTSLSDLNDPIIL
                     SSDDDDDNDRTNRRESISPQPADSACSSPAPSTGKVEAALNENTCRAERELRSIPEDS
                     ELNTVTLPRKARMKDQFGNSIINTPLKRRKVFSQEPPDALALSCQSSFDSVILNCRSI
                     RVGTLFRLLIEPVIFCLDFIKIQLDEPDHDPVEIILNTSDLTKCEWCNVRKLPVVFLQ
                     AIPAVYQKLSIQLQMNKEDKVWNDCKGVNKLTNLEEQYIILIFQNGLDPPANMVFESI
                     INEIGIKNNISNFFAKIPFEEANGRLVACTRTYEESIKGSCGQKENKIKTVSFESKIQ
                     LRSKQEFQFFDEEEETGENHTIFIGPVEKLIVYPPPPAKGGISVTNEDLHCLNEGEFL
                     NDVIIDFYLKYLVLEKLKKEDADRIHIFSSFFYKRLNQRERRNHETTNLSIQQKRHGR
                     VKTWTRHVDIFEKDFIFVPLNEAAHWFLAVVCFPGLEKPKYEPNPHYHENAVIQKCST
                     VEDSCISSSASEMESCSQNSSAKPVIKKMLNKKHCIAVIDSNPGQEESDPRYKRNICS
                     VKYSVKKINHTASENEEFNKGESTSQKVADRTKSENGLQNESLSSTHHTDGLSKIRLN
                     YSDESPEAGKMLEDELVDFSEDQDNQDDSSDDGFLADDNCSSEIGQWHLKPTICKQPC
                     ILLMDSLRGPSRSNVVKILREYLEVEWEVKKGSKRSFSKDVMKGSNPKVPQQNNFSDC
                     GVYVLQYVESFFENPILSFELPMNLANWFPPPRMRTKREEIRNIILKLQEDQSKEKRK
                     HKDTYSTEAPLGEGTEQYVNSISD"
     exon            698..791
                     /gene="SENP6"
                     /gene_synonym="SSP1; SUSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            792..852
                     /gene="SENP6"
                     /gene_synonym="SSP1; SUSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            853..998
                     /gene="SENP6"
                     /gene_synonym="SSP1; SUSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            999..1103
                     /gene="SENP6"
                     /gene_synonym="SSP1; SUSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1104..1124
                     /gene="SENP6"
                     /gene_synonym="SSP1; SUSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1125..1195
                     /gene="SENP6"
                     /gene_synonym="SSP1; SUSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1196..1341
                     /gene="SENP6"
                     /gene_synonym="SSP1; SUSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1342..1639
                     /gene="SENP6"
                     /gene_synonym="SSP1; SUSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1640..1869
                     /gene="SENP6"
                     /gene_synonym="SSP1; SUSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1870..2037
                     /gene="SENP6"
                     /gene_synonym="SSP1; SUSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2038..2071
                     /gene="SENP6"
                     /gene_synonym="SSP1; SUSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2072..2266
                     /gene="SENP6"
                     /gene_synonym="SSP1; SUSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2267..2493
                     /gene="SENP6"
                     /gene_synonym="SSP1; SUSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2494..2603
                     /gene="SENP6"
                     /gene_synonym="SSP1; SUSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2604..2720
                     /gene="SENP6"
                     /gene_synonym="SSP1; SUSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2721..2840
                     /gene="SENP6"
                     /gene_synonym="SSP1; SUSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2841..2933
                     /gene="SENP6"
                     /gene_synonym="SSP1; SUSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2934..3361
                     /gene="SENP6"
                     /gene_synonym="SSP1; SUSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3362..3465
                     /gene="SENP6"
                     /gene_synonym="SSP1; SUSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3466..3554
                     /gene="SENP6"
                     /gene_synonym="SSP1; SUSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3555..3623
                     /gene="SENP6"
                     /gene_synonym="SSP1; SUSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3624..3774
                     /gene="SENP6"
                     /gene_synonym="SSP1; SUSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3775..6671
                     /gene="SENP6"
                     /gene_synonym="SSP1; SUSP1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4271..4276
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SENP6"
                     /gene_synonym="SSP1; SUSP1"
                     /note="hexamer: AATAAA"
     regulatory      4277..4282
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SENP6"
                     /gene_synonym="SSP1; SUSP1"
                     /note="hexamer: AAGAAA"
     polyA_site      4294
                     /gene="SENP6"
                     /gene_synonym="SSP1; SUSP1"
                     /note="major polyA site"
     polyA_site      4299
                     /gene="SENP6"
                     /gene_synonym="SSP1; SUSP1"
     regulatory      5590..5595
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SENP6"
                     /gene_synonym="SSP1; SUSP1"
                     /note="hexamer: AATAAA"
     polyA_site      5609
                     /gene="SENP6"
                     /gene_synonym="SSP1; SUSP1"
                     /note="major polyA site"
     regulatory      6490..6495
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SENP6"
                     /gene_synonym="SSP1; SUSP1"
                     /note="hexamer: ATTACA"
     polyA_site      6516
                     /gene="SENP6"
                     /gene_synonym="SSP1; SUSP1"
     regulatory      6646..6651
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SENP6"
                     /gene_synonym="SSP1; SUSP1"
                     /note="hexamer: AATAAA"
     polyA_site      6671
                     /gene="SENP6"
                     /gene_synonym="SSP1; SUSP1"
ORIGIN      
        1 agcggcggcg gctggagctg ctgtggcgac cgacgcgagg cggtggcaga ggagacccac
       61 ccctgtccac atggacagtc gcaaaggcct ccgctgatgc attcacgcct gggcggggtg
      121 ggcggacggc cgtagcggcg gcggctgcag aacgagctag gggcctgggg gcgcctgacg
      181 gtcgcagaga cctcgccgct ccggcgcggc gggtgcggcc attttacggc ctgggacgaa
      241 gggaggcgtg tttgtgtgct cgctttcatt ctcctttctt gggaacccac ggctggggga
      301 agtttctcag gcagcctggg tgggcggtgg atggggagtc gtgggccgag aggaaccggg
      361 cccgggaagc gccgtcgtcg tcgtcgccgg tcgcgttccc ccggagaggc ctgagaagct
      421 cgggccgcgg gcctcgctgc ccgccagccc gcggacaggc ccgggcgcgc ctggcctgcc
      481 tttgtatagg cccgtctgaa cgtgggagcg cagcccgcct gacggctgag cccgaggccc
      541 gcaaccctgc ggcgtctacc ctcctccggc gcggcccctc atcccggcga gcacggcggc
      601 ggtgtgggcc atggattaag aaggaggcgg cgtgggagga ggaagatggc ggccggcaag
      661 agcggcggta gcgcagggga gattactttt ctggaagctt tggctagatc agagtctaag
      721 agagatggag gttttaaaaa taattggagc tttgatcatg aagaagaaag tgaaggagat
      781 acagataaag atgggacaaa tctgctcagt gtggatgaag atgaggattc tgaaacctca
      841 aaaggaaaaa agttaaatcg tcgatctgaa attgttgcta atagctctgg tgaattcatc
      901 ttgaagacat atgtaagacg aaacaagtct gaaagtttta aaactttgaa aggcaaccca
      961 attggactta acatgttgag caacaataag aaattgagtg aaaatacgca aaatacgtca
     1021 ttatgttctg gaactgtagt tcatggtaga cgttttcatc atgctcatgc acagatacca
     1081 gtagtaaaaa cagcagccca aagcagtctg gaccgaaaag aaaggaaaga atacccacct
     1141 catgtccaaa aagttgaaat taatcctgta aggttaagtc ggctccaagg tgttgaacgt
     1201 ataatgaaga aaacagaaga gtccgaatca caagtggagc ctgaaattaa gaggaaagta
     1261 caacagaaac gacactgtag tacctatcag cctactcctc ctctatctcc tgcttcaaaa
     1321 aaatgtttaa cccatttaga ggatttgcaa agaaattgca gacaagctat tactttgaat
     1381 gagtctactg gaccattatt aagaacgtca attcatcaga attctggagg acagaagtca
     1441 caaaacacag gattaacaac caagaagttt tatggcaaca atgtggaaaa ggttccaatt
     1501 gatattattg tgaattgtga tgacagtaaa cacacttatt tacagactaa tggaaaagtc
     1561 attttacctg gggcaaaaat acccaaaatc acaaacttga aagaaaggaa aacaagtttg
     1621 tcagacctaa atgatccaat cattttgtcc agtgatgatg atgatgacaa cgacagaact
     1681 aacagaagag aaagcatatc tcctcagcct gctgattcag catgttcttc ccctgcacca
     1741 tccactggaa aagtagaagc agcgctaaat gaaaatactt gcagagcaga gcgtgaacta
     1801 cgaagcattc cagaagactc agagttaaat acagttacat tgccaagaaa agcaagaatg
     1861 aaagaccagt ttggcaattc tattatcaac acacctctga aacgtcgtaa agtgttttct
     1921 caagaacctc cagatgcttt agctttaagc tgccaaagtt cctttgacag tgtcatttta
     1981 aactgtcgaa gtatacgagt aggaacactc ttccggctgt taatagagcc tgtaattttt
     2041 tgtttagatt ttatcaagat acagctagac gaaccagacc atgatcctgt agagattata
     2101 ttaaatacct ctgatctaac taaatgtgaa tggtgtaatg tccgaaaatt acctgtagtg
     2161 tttcttcaag caattccagc agtttatcaa aagctgagca tccaactgca aatgaataag
     2221 gaggataaag tttggaatga ttgtaaagga gtaaataaat taacaaattt agaagaacaa
     2281 tatataattt taatttttca aaatggcctt gatcctccgg caaatatggt atttgaaagt
     2341 atcattaatg aaattggtat aaagaataac atctccaatt tttttgcgaa aattcccttt
     2401 gaagaagcta atggcagact tgttgcctgt acaagaacct atgaagagag catcaaagga
     2461 agttgtgggc aaaaggaaaa caaaattaaa actgtatcat ttgaatctaa aatacaactt
     2521 agaagcaaac aagaatttca gttttttgat gaagaagaag aaactggaga aaaccacacc
     2581 atcttcattg gcccagtaga aaagttgata gtatatccac cacctccagc taagggaggc
     2641 atctctgtta ccaatgagga cctgcactgt ctaaatgaag gagaattttt aaatgatgtt
     2701 attatagact tttatttgaa atacttggtg cttgaaaaac tgaagaagga agacgctgac
     2761 cgaattcata tattcagttc ttttttctat aaacgcctta atcagagaga gaggagaaat
     2821 catgaaacaa ctaatctgtc aatacagcaa aaacggcatg ggagagtaaa aacatggacc
     2881 cggcacgtag atatttttga gaaggatttt atttttgtac cccttaatga agctgcacac
     2941 tggtttttgg ctgttgtttg tttccccggt ttggaaaaac caaagtatga acctaatcct
     3001 cattaccatg aaaatgctgt catacagaaa tgttcaactg tagaggacag ttgtatttct
     3061 tcttcagcca gtgaaatgga gagttgttca caaaactctt ctgccaagcc tgtaattaag
     3121 aagatgctaa acaaaaaaca ttgcatagct gtaattgatt ccaatcctgg gcaggaagaa
     3181 agtgaccctc gttataagag aaacatatgc agtgtaaaat acagtgtgaa aaaaataaat
     3241 catactgcga gtgaaaatga agaattcaat aaaggagaat ctacatccca gaaagttgct
     3301 gataggacta aaagtgagaa tggcctacag aatgaaagtt taagttccac acatcataca
     3361 gatggcttaa gcaaaatcag actaaactat agcgatgaat cacctgaagc tggtaaaatg
     3421 cttgaagatg aactcgtcga cttctcagaa gatcaggata accaggatga tagcagtgac
     3481 gatggattcc tcgctgatga caactgcagt tcagaaatag gacagtggca tttaaagcct
     3541 actatctgta aacaaccttg tatcctactt atggactcac tccgaggccc ttctcggtca
     3601 aatgttgtca aaattttaag agagtattta gaagtggaat gggaagttaa aaaaggaagc
     3661 aaaagaagtt tttccaaaga tgttatgaag ggctctaatc caaaagtacc acagcaaaac
     3721 aacttcagtg actgtggtgt atatgtattg cagtatgtag agagcttttt tgagaatcca
     3781 attctcagtt ttgaactacc tatgaatttg gcaaactggt ttcctccacc aagaatgaga
     3841 acaaaaagag aagaaatccg aaacataatt ctgaagctac aggaagatca gagcaaagag
     3901 aaaagaaagc ataaggacac ttactcaaca gaagcacctt taggcgaagg aacagaacaa
     3961 tatgtcaata gtatctcaga ttgaccattt ctgttacttg tcatttctac tttcagaaac
     4021 taaatgactt tcaaatttgg gtatagacaa taaagaactg aagtgctcac tactcagtga
     4081 tttggaaatt ttgatgcttg tataaatgtc agataattaa tttccaaagg cgtatgtatt
     4141 aagtaaaagt ctgtaaatat gttaatgagg ccaatttttc cagcatttat aattattttt
     4201 ttcacttgtt aggaagcttt tgttatgtat tttctgttaa tagtacctaa aattgcaact
     4261 tctaaacaca aataaaaaga aaatatttat aggaggaaat gattaatttg atattcttta
     4321 gtgaacttgt ttaattcctc agtgggtgtg acatatttca tgggaatatt caaatatcta
     4381 tggtaatatt ttgacccttt atatttgttc taaaataagt caaaatgtga aaataatatt
     4441 aaatctaaga tattttgaac taagcatctt tatatgcttg tgtaacagga acaaagtaac
     4501 agcctttcaa ttcatatact gccttgtgtt cagtgaaccc aagaaatgta ataaatattt
     4561 gtaattttac acaaatattt aagaggaaag agtattaaga gcaattcaaa aaaagtaacc
     4621 ttatactact aaaaaaaaaa ttcttgcata tattatcatc aaatgcattt ttgaagacat
     4681 caaagactca ggttaaaact attttggtaa gtgcagcttg aatttcaaat atcccgtgtt
     4741 acctttctct attacagctt aaagtatgct acaatctgtg tcatatagtt aattgataag
     4801 catttttaat ctgtgtaaac acaggaattt aaataggaat ttactatttt tttataaagc
     4861 ttttgctatt ttttcattgc tcattttgtt cttattattt tgataaagta tcagactttt
     4921 tgcttgagtt cttcagtgta aattattttt tacatgtaaa agtactgtat tcaaccagtt
     4981 gcataataca gcaaaaatct ttgaattccc ctttaaaata actaaaattt gatggtttcc
     5041 atgacagatt tatagcacat atagggatct tgcatgtatc tgcagaaact tcattatttt
     5101 tcaaatgaaa tggtgtatac ttctttcaaa tgaactttga gacttgaaac atattttagt
     5161 cattttttgt taaatattga atttttaaat acacatatat caaaaatagt tgagaataaa
     5221 aagaaagcct aatcatcagc aattatttta tttttaattt ggccatctct gacattgcag
     5281 tcaatatctt agggtatttt cttgaattta ataaagtccc atgggtgggt tcagggtgaa
     5341 tgggagtatt aacaacaacc aaaaaatatc tattgagaca cagtagagtc tcagtaagag
     5401 aaatattaaa atgtaagaaa ggtcaccatt agtgatatca actgtagttt gttactttga
     5461 actatatttc tgattaactc tttatagtaa taacttagag ctgttagctc agatatctta
     5521 attagctcat atgaaaaaca agtttaattt tattatttac tgaacatggc aaaatgcttt
     5581 tcaatctata ataaaactgg gataaaaaat tgaagttgtt ttttttaaaa cctaatagca
     5641 ataagcaaaa ttggcataca taattttttt aatgaactat attttgtcag aatctgaagg
     5701 tactgaaaaa ctattctaaa atgcctactt gtttttgcat taatttgtaa tgcttacatt
     5761 ttgcaccctt agataatgtt tgataagata aaaatataat ttatcccttg taccaactca
     5821 agtcaatcag attttaacat gaaaaatata gattaatgta tgtcagcttt ctagaggtag
     5881 aatactcagc tatttgatgg catttttccc accccatgtg aaattttatt tttggaagtt
     5941 atgctagtga cattgcaata tatattgaaa tcttcggagg ctgccaggtt gagcttcaaa
     6001 actagacttg agaatctttc gtgtatatgc acattcactt gataattgtg tattctatgt
     6061 agtttaaagt gggatcaagc cttttaaaat gtgttattag tacttagaag ttgtaaaaca
     6121 gaacttatag aggtttgtta ctagacactg aaactgcatg acaagtatct ggtgtcttaa
     6181 ctaattagat agatctgttg tattggtttt cttgttggat agaataacag ttaatcattt
     6241 ttagaagagt tttagattac ttgccataaa atttgtatgt ctcagctctt gttggtttag
     6301 gagcagaagt ggtgtttagt cccttcccgt taatgttgct gtgctacttc agacagctga
     6361 aaacttaatg gtattatttc aagttcacat aaccctaaaa aaggttttct ttgtgtagta
     6421 acttgtgctt aaacttttag tcaattaaat gtaggaggtt ttatttgaaa acataaaatg
     6481 attctctaaa ttacatcttg ttttaagcca ccaaaatgaa tgcagtaatt tttctttaaa
     6541 aaaatgatct ctgaaaactg tgagacaatg taaaaagaat aagttttaat tcccccaaaa
     6601 tcaaaaattg tacttaaaat ctcagacaat tcactgaaca ggaataataa agaacatatt
     6661 ttaatatata a
//
