LOCUS       NM_012431               7273 bp    mRNA    linear   PRI 08-OCT-2024
DEFINITION  Homo sapiens semaphorin 3E (SEMA3E), transcript variant 1, mRNA.
ACCESSION   NM_012431 XM_376647 XM_379884
VERSION     NM_012431.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7273)
  AUTHORS   Scarpa,E.S., Giordani,C., Antonelli,A., Petrelli,M., Balercia,G.,
            Silvetti,F., Pieroni,A., Sabbatinelli,J., Rippo,M.R., Olivieri,F.
            and Matacchione,G.
  TITLE     The Combination of Natural Molecules Naringenin, Hesperetin,
            Curcumin, Polydatin and Quercetin Synergistically Decreases SEMA3E
            Expression Levels and DPPIV Activity in In Vitro Models of Insulin
            Resistance
  JOURNAL   Int J Mol Sci 24 (9), 8071 (2023)
   PUBMED   37175783
  REMARK    GeneRIF: The Combination of Natural Molecules Naringenin,
            Hesperetin, Curcumin, Polydatin and Quercetin Synergistically
            Decreases SEMA3E Expression Levels and DPPIV Activity in In Vitro
            Models of Insulin Resistance.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7273)
  AUTHORS   Hagihara,K., Haraguchi,N., Nishimura,J., Yasueda,A., Fujino,S.,
            Ogino,T., Takahashi,H., Miyoshi,N., Uemura,M., Matsuda,C.,
            Mizushima,T., Yamamoto,H., Mori,M., Doki,Y. and Eguchi,H.
  TITLE     PLXND1/SEMA3E Promotes Epithelial-Mesenchymal Transition Partly via
            the PI3K/AKT-Signaling Pathway and Induces Heterogenity in
            Colorectal Cancer
  JOURNAL   Ann Surg Oncol 29 (12), 7435-7445 (2022)
   PUBMED   35917012
  REMARK    GeneRIF: PLXND1/SEMA3E Promotes Epithelial-Mesenchymal Transition
            Partly via the PI3K/AKT-Signaling Pathway and Induces Heterogenity
            in Colorectal Cancer.
REFERENCE   3  (bases 1 to 7273)
  AUTHORS   Eissa,N., Hussein,H., Diarra,A., Elgazzar,O., Gounni,A.S.,
            Bernstein,C.N. and Ghia,J.E.
  TITLE     Semaphorin 3E regulates apoptosis in the intestinal epithelium
            during the development of colitis
  JOURNAL   Biochem Pharmacol 166, 264-273 (2019)
   PUBMED   31170375
  REMARK    GeneRIF: SEMA3E is a novel regulator in intestinal inflammation
            that regulates intestinal epithelium apoptosis.
REFERENCE   4  (bases 1 to 7273)
  AUTHORS   Kermarrec,L., Eissa,N., Wang,H., Kapoor,K., Diarra,A., Gounni,A.S.,
            Bernstein,C.N. and Ghia,J.E.
  TITLE     Semaphorin-3E attenuates intestinal inflammation through the
            regulation of the communication between splenic CD11C+ and CD4+
            CD25- T-cells
  JOURNAL   Br J Pharmacol 176 (9), 1235-1250 (2019)
   PUBMED   30736100
  REMARK    GeneRIF: The SEMA3E is reduced in colitis and modulates colonic
            inflammation by regulating the interaction between CD11c(+) and
            CD4(+) CD25(-) T-cells via an NF-kappaB-dependent mechanism.
REFERENCE   5  (bases 1 to 7273)
  AUTHORS   Movassagh,H., Koussih,L., Shan,L. and Gounni,A.S.
  TITLE     The regulatory role of semaphorin 3E in allergic asthma
  JOURNAL   Int J Biochem Cell Biol 106, 68-73 (2019)
   PUBMED   30447428
  REMARK    GeneRIF: recently discovered regulatory role of semaphorin3E in
            modulating immune cells and structural cells function in the
            airways. These findings support the concept of
            semaphorin3E/plexinD1 axis as a therapeutic target in allergic
            asthma.
            Review article
REFERENCE   6  (bases 1 to 7273)
  AUTHORS   Gu,C., Yoshida,Y., Livet,J., Reimert,D.V., Mann,F., Merte,J.,
            Henderson,C.E., Jessell,T.M., Kolodkin,A.L. and Ginty,D.D.
  TITLE     Semaphorin 3E and plexin-D1 control vascular pattern independently
            of neuropilins
  JOURNAL   Science 307 (5707), 265-268 (2005)
   PUBMED   15550623
REFERENCE   7  (bases 1 to 7273)
  AUTHORS   Pascual,M., Pozas,E. and Soriano,E.
  TITLE     Role of class 3 semaphorins in the development and maturation of
            the septohippocampal pathway
  JOURNAL   Hippocampus 15 (2), 184-202 (2005)
   PUBMED   15386596
REFERENCE   8  (bases 1 to 7273)
  AUTHORS   Lalani,S.R., Safiullah,A.M., Molinari,L.M., Fernbach,S.D.,
            Martin,D.M. and Belmont,J.W.
  TITLE     SEMA3E mutation in a patient with CHARGE syndrome
  JOURNAL   J Med Genet 41 (7), e94 (2004)
   PUBMED   15235037
  REMARK    GeneRIF: The translocation envolving chromosomes 2 and 7 with
            SEMA3E within 200 kb of the translocation breakpoint on 7q21.11 in
            a patient with CHARGE syndrome.
REFERENCE   9  (bases 1 to 7273)
  AUTHORS   Christensen,C.R., Klingelhofer,J., Tarabykina,S., Hulgaard,E.F.,
            Kramerov,D. and Lukanidin,E.
  TITLE     Transcription of a novel mouse semaphorin gene, M-semaH, correlates
            with the metastatic ability of mouse tumor cell lines
  JOURNAL   Cancer Res 58 (6), 1238-1244 (1998)
   PUBMED   9515811
REFERENCE   10 (bases 1 to 7273)
  AUTHORS   Balasubramanian,R. and Crowley,W.F. Jr.
  TITLE     Isolated Gonadotropin-Releasing Hormone (GnRH) Deficiency
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301509
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB215551.1, BC152458.1,
            DA722912.1, BC140706.1, AC079799.7 and AW450461.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Oct 1, 2018 this sequence version replaced NM_012431.2.
            
            Summary: Semaphorins are a large family of conserved secreted and
            membrane associated proteins which possess a semaphorin (Sema)
            domain and a PSI domain (found in plexins, semaphorins and
            integrins) in the N-terminal extracellular portion. Based on
            sequence and structural similarities, semaphorins are put into
            eight classes: invertebrates contain classes 1 and 2, viruses have
            class V, and vertebrates contain classes 3-7. Semaphorins serve as
            axon guidance ligands via multimeric receptor complexes, some (if
            not all) containing plexin proteins. This gene encodes a class 4
            semaphorin. This gene encodes a class 3 semaphorin. Multiple
            transcript variants encoding different isoforms have been found for
            this gene.[provided by RefSeq, May 2010].
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB002329.1, BC152458.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2467144 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000643230.2/ ENSP00000496491.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-131               DB215551.1         37-167
            132-228             BC152458.1         13-109
            229-570             DA722912.1         229-570
            571-2093            BC152458.1         452-1974
            2094-4820           BC140706.1         1653-4379
            4821-6943           AC079799.7         121936-124058       c
            6944-7273           AW450461.1         16-345              c
FEATURES             Location/Qualifiers
     source          1..7273
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7q21.11"
     gene            1..7273
                     /gene="SEMA3E"
                     /gene_synonym="coll-5; M-SEMAH; M-SemaK; SEMAH"
                     /note="semaphorin 3E"
                     /db_xref="GeneID:9723"
                     /db_xref="HGNC:HGNC:10727"
                     /db_xref="MIM:608166"
     exon            1..712
                     /gene="SEMA3E"
                     /gene_synonym="coll-5; M-SEMAH; M-SemaK; SEMAH"
                     /inference="alignment:Splign:2.1.0"
     CDS             598..2925
                     /gene="SEMA3E"
                     /gene_synonym="coll-5; M-SEMAH; M-SemaK; SEMAH"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; sema domain, immunoglobulin domain (Ig), short
                     basic domain, secreted, (semaphorin) 3E"
                     /codon_start=1
                     /product="semaphorin-3E isoform 1 precursor"
                     /protein_id="NP_036563.1"
                     /db_xref="CCDS:CCDS34674.1"
                     /db_xref="GeneID:9723"
                     /db_xref="HGNC:HGNC:10727"
                     /db_xref="MIM:608166"
                     /translation="MASAGHIITLLLWGYLLELWTGGHTADTTHPRLRLSHKELLNLN
                     RTSIFHSPFGFLDLHTMLLDEYQERLFVGGRDLVYSLSLERISDGYKEIHWPSTALKM
                     EECIMKGKDAGECANYVRVLHHYNRTHLLTCGTGAFDPVCAFIRVGYHLEDPLFHLES
                     PRSERGRGRCPFDPSSSFISTLIGSELFAGLYSDYWSRDAAIFRSMGRLAHIRTEHDD
                     ERLLKEPKFVGSYMIPDNEDRDDNKVYFFFTEKALEAENNAHAIYTRVGRLCVNDVGG
                     QRILVNKWSTFLKARLVCSVPGMNGIDTYFDELEDVFLLPTRDHKNPVIFGLFNTTSN
                     IFRGHAICVYHMSSIRAAFNGPYAHKEGPEYHWSVYEGKVPYPRPGSCASKVNGGRYG
                     TTKDYPDDAIRFARSHPLMYQAIKPAHKKPILVKTDGKYNLKQIAVDRVEAEDGQYDV
                     LFIGTDNGIVLKVITIYNQEMESMEEVILEELQIFKDPVPIISMEISSKRQQLYIGSA
                     SAVAQVRFHHCDMYGSACADCCLARDPYCAWDGISCSRYYPTGTHAKRRFRRQDVRHG
                     NAAQQCFGQQFVGDALDKTEEHLAYGIENNSTLLECTPRSLQAKVIWFVQKGRETRKE
                     EVKTDDRVVKMDLGLLFLRLHKSDAGTYFCQTVEHSFVHTVRKITLEVVEEEKVEDMF
                     NKDDEEDRHHRMPCPAQSSISQGAKPWYKEFLQLIGYSNFQRVEEYCEKVWCTDRKRK
                     KLKMSPSKWKYANPQEKKLRSKPEHYRLPRHTLDS"
     exon            713..873
                     /gene="SEMA3E"
                     /gene_synonym="coll-5; M-SEMAH; M-SemaK; SEMAH"
                     /inference="alignment:Splign:2.1.0"
     exon            874..933
                     /gene="SEMA3E"
                     /gene_synonym="coll-5; M-SEMAH; M-SemaK; SEMAH"
                     /inference="alignment:Splign:2.1.0"
     exon            934..1053
                     /gene="SEMA3E"
                     /gene_synonym="coll-5; M-SEMAH; M-SemaK; SEMAH"
                     /inference="alignment:Splign:2.1.0"
     exon            1054..1147
                     /gene="SEMA3E"
                     /gene_synonym="coll-5; M-SEMAH; M-SemaK; SEMAH"
                     /inference="alignment:Splign:2.1.0"
     exon            1148..1267
                     /gene="SEMA3E"
                     /gene_synonym="coll-5; M-SEMAH; M-SemaK; SEMAH"
                     /inference="alignment:Splign:2.1.0"
     exon            1268..1410
                     /gene="SEMA3E"
                     /gene_synonym="coll-5; M-SEMAH; M-SemaK; SEMAH"
                     /inference="alignment:Splign:2.1.0"
     exon            1411..1525
                     /gene="SEMA3E"
                     /gene_synonym="coll-5; M-SEMAH; M-SemaK; SEMAH"
                     /inference="alignment:Splign:2.1.0"
     exon            1526..1595
                     /gene="SEMA3E"
                     /gene_synonym="coll-5; M-SEMAH; M-SemaK; SEMAH"
                     /inference="alignment:Splign:2.1.0"
     exon            1596..1740
                     /gene="SEMA3E"
                     /gene_synonym="coll-5; M-SEMAH; M-SemaK; SEMAH"
                     /inference="alignment:Splign:2.1.0"
     exon            1741..1963
                     /gene="SEMA3E"
                     /gene_synonym="coll-5; M-SEMAH; M-SemaK; SEMAH"
                     /inference="alignment:Splign:2.1.0"
     exon            1964..2055
                     /gene="SEMA3E"
                     /gene_synonym="coll-5; M-SEMAH; M-SemaK; SEMAH"
                     /inference="alignment:Splign:2.1.0"
     exon            2056..2097
                     /gene="SEMA3E"
                     /gene_synonym="coll-5; M-SEMAH; M-SemaK; SEMAH"
                     /inference="alignment:Splign:2.1.0"
     exon            2098..2264
                     /gene="SEMA3E"
                     /gene_synonym="coll-5; M-SEMAH; M-SemaK; SEMAH"
                     /inference="alignment:Splign:2.1.0"
     exon            2265..2332
                     /gene="SEMA3E"
                     /gene_synonym="coll-5; M-SEMAH; M-SemaK; SEMAH"
                     /inference="alignment:Splign:2.1.0"
     exon            2333..2472
                     /gene="SEMA3E"
                     /gene_synonym="coll-5; M-SEMAH; M-SemaK; SEMAH"
                     /inference="alignment:Splign:2.1.0"
     exon            2473..7273
                     /gene="SEMA3E"
                     /gene_synonym="coll-5; M-SEMAH; M-SemaK; SEMAH"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3518..3523
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SEMA3E"
                     /gene_synonym="coll-5; M-SEMAH; M-SemaK; SEMAH"
                     /note="hexamer: ATTAAA"
     polyA_site      3537
                     /gene="SEMA3E"
                     /gene_synonym="coll-5; M-SEMAH; M-SemaK; SEMAH"
     regulatory      7250..7255
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SEMA3E"
                     /gene_synonym="coll-5; M-SEMAH; M-SemaK; SEMAH"
                     /note="hexamer: AATAAA"
     polyA_site      7273
                     /gene="SEMA3E"
                     /gene_synonym="coll-5; M-SEMAH; M-SemaK; SEMAH"
                     /note="major polyA site"
ORIGIN      
        1 aaccctgcct gaactttcct gtaaacagcc actgacagac tgcatccctg ctgggtggat
       61 cctgcgactc ctagagggaa tggcacttct tgtttttaat tagcaaggtg aaaggtgaat
      121 taaaactagc agtttggcaa gtcagtgcaa gaggctgact tctgagaggc ttccaggagc
      181 ccgaagagag gacctccacg ggagaaggga gtgcgtgtgc tcggttttgt ttttttctct
      241 cttttttttt tttttttctg aatgaacagc tttgcccaag tgactgaaaa atacagcttc
      301 ttcctgaatc taccggcgta gttgctgaag agcgctctag acaggacatg gctctgaaga
      361 ctcactcttt ggaatgtcct cttgctcccg gcttataaac aactgtcccg aggaaagaaa
      421 ggttttacat agccaaatac agcctgacaa atggcacttc ggaactgtgc tttctgatga
      481 caacgcgttc gatttctgac aaagcctctc gcacgctgcc cctggaggga agtcctaagt
      541 aaaactcaga ccctccttaa agtgaggagc gagggcttgg acggtgaaca cggcagcatg
      601 gcatccgcgg ggcacattat caccttgctc ctgtggggtt acttactgga gctttggaca
      661 ggaggtcata cagctgatac tacccacccc cggttacgcc tgtcacataa agagctcttg
      721 aatctgaaca gaacatcaat atttcatagc ccttttggat ttcttgatct ccatacaatg
      781 ctgctggatg aatatcaaga gaggctcttc gtgggaggca gggaccttgt atattccctc
      841 agcttggaga gaatcagtga cggctataaa gagatacact ggccgagtac agctctaaaa
      901 atggaagaat gcataatgaa gggaaaagat gcgggtgaat gtgcaaatta tgttcgggtt
      961 ttgcatcact ataacaggac acaccttctg acctgtggta ctggagcttt tgatccagtt
     1021 tgtgccttca tcagagttgg atatcatttg gaggatcctc tgtttcacct ggaatcaccc
     1081 agatctgaga gaggaagggg cagatgtcct tttgacccca gctcctcctt catctccact
     1141 ttaattggta gtgaattgtt tgctggactc tacagtgact actggagcag agacgctgcg
     1201 atcttccgca gcatggggcg actggcccat atccgcactg agcatgacga tgagcgtctg
     1261 ttgaaagaac caaaatttgt aggttcatac atgattcctg acaatgaaga cagagatgac
     1321 aacaaagtat atttcttttt tactgagaag gcactggagg cagaaaacaa tgctcacgca
     1381 atttacacca gggtcgggcg actctgtgtg aatgatgtag gagggcagag aatactggtg
     1441 aataagtgga gcactttcct aaaagcgaga ctcgtttgct cagtaccagg aatgaatgga
     1501 attgacacat attttgatga attagaggac gtttttttgc tacctaccag agatcataag
     1561 aatccagtga tatttggact ctttaacact accagtaata tttttcgagg gcatgctata
     1621 tgtgtctatc acatgtctag cattcgggca gccttcaacg gaccatatgc acataaggaa
     1681 ggacctgaat accactggtc agtctatgaa ggaaaagtcc cttatccaag gcctggttct
     1741 tgtgccagca aagtaaatgg agggagatac ggaaccacca aggactatcc tgatgatgcc
     1801 atccgatttg caagaagtca tccactaatg taccaggcca taaaacctgc ccataaaaaa
     1861 ccaatattgg taaaaacaga tggaaaatat aacctgaaac aaatagcagt agatcgagtg
     1921 gaagctgagg atggccaata tgacgtcttg tttattggga cagataatgg aattgtgctg
     1981 aaagtaatca caatttacaa ccaagaaatg gaatcaatgg aagaagtaat tctagaagaa
     2041 cttcagatat tcaaggatcc agttcctatt atttctatgg agatttcttc aaagcggcaa
     2101 cagctgtata ttggatctgc ttctgctgtg gctcaagtca gattccatca ctgtgacatg
     2161 tatggaagtg cttgtgctga ctgctgcctg gctcgagacc cttactgtgc ctgggatggc
     2221 atatcctgct cccggtatta cccaacaggc acacatgcaa aaaggcgttt ccggagacaa
     2281 gatgttcgac atggaaatgc agctcagcag tgctttggac aacagtttgt tggggatgct
     2341 ttggataaga ctgaagaaca tctggcttat ggcatagaga acaacagtac tttgctggaa
     2401 tgtaccccac gatctttaca agcgaaagtt atctggtttg tacagaaagg acgtgagaca
     2461 agaaaagagg aggtgaagac agatgacaga gtggttaaga tggaccttgg tttactcttc
     2521 ctaaggttac acaaatcaga tgctgggacc tatttttgcc agacagtaga gcatagcttt
     2581 gtccatacgg tccgtaaaat caccttggag gtagtggaag aggagaaagt cgaggatatg
     2641 tttaacaagg acgatgagga ggacaggcat cacaggatgc cttgtcctgc tcagagtagc
     2701 atctcgcagg gagcaaaacc atggtacaag gaattcttgc agctgatcgg ttatagcaac
     2761 ttccagagag tggaagaata ctgcgagaaa gtatggtgca cagatagaaa gaggaaaaag
     2821 cttaaaatgt caccctccaa gtggaagtat gccaaccctc aggaaaagaa gctccgttcc
     2881 aaacctgagc attaccgcct gcccaggcac acgctggact cctgatgggg tgagactatc
     2941 tactgtcttt tgaagaattt atatttggaa agtaaaaaag taaaaaaata aatcatccaa
     3001 cttctttgca ttacttaaaa gagatttctg taatacagga atgactatga aggtgttata
     3061 ataaattatt ctacatactc atttgactgg ataaacttta cataaaatta actaattttt
     3121 taaataaatg cattgcttaa tggtttctca ttatgtttat caaaaaacaa ctgtagctgt
     3181 tattttcagt acttggctgc ttttctgtga aaattattat tttacttttg gaagacaaga
     3241 ttattagaat attgaagaaa aattggagac ttataatcat ggtaaatata aaactaaata
     3301 tgttttaata tttctgaatt tttcttttcc atcacaatgt aagatatgca gaatacaaga
     3361 tactttggca ttctcatgtg aactttctgt actctttaag gattatttta ttagtgttgt
     3421 ttaagccatg agtgttaagt agcaggtgtg ttgtgagtgc tgtaacccat gaaaggaaaa
     3481 atgtcattct gaggcttgtg cccttcgtaa aatattcatt aaagtacatt cacactattt
     3541 ttgctttata acacagtctt taattttcac tcactgtgga aataaaaact aaggtaactt
     3601 ctcagaaaga tatcaaatct cagaaagaat gtcaaatcag atgaagttat agttaggatt
     3661 ctaactactg taaaagattt ttgcttccct cttgtggtaa aaaaaattat attctcacac
     3721 atttcttttt tctctacaga cggatatctg tttaggaaag atttgaaagc agattatcag
     3781 taggtacatg gatacatcaa gttcatttgc agaaacaaat aactgaaata aaaaacatgt
     3841 taatccttgt atcatacttt aatatgaaag tattgtttat agataattta tctcacaagt
     3901 caaaaatgaa gattttgcag cactgaaaat ctattaaagc tccaaatttt aagtttctaa
     3961 ataatcttcg ctgaaatcta aaatatacta taacaaccgt gttttatttg tgaaaaaaat
     4021 attaaagtga tttgctctca aatatcaaat tttcttctct cttttatatt aagagacaga
     4081 aaattgtttc atgagttcac ttaactactg agatattcag agcattttta cctctctctt
     4141 aaatgttata aaaaacaatt gtatttttaa gaatgtttat ttatcaaagt ctttccttct
     4201 tctattaaat atttagcaat tacctttcta aaatatgaaa ttttgtaaga tgttttcacc
     4261 taaataaaaa ttgaaagcaa gtggattaca caggagaacc attatgaaca tttatttaga
     4321 tattaatctt aaacagtgtt tatttcagtt ttcaaagtta gcttataggt tatacattta
     4381 agttaaagtg ctcataatca cttgcaattt cattgtaaaa tgaacaaata cataaatatt
     4441 ttaagaaaaa tttaagttta ttcagataag tcaccatgct tcaaaagatc taagaaatgc
     4501 aaatatactg aaaattgaca tcctctgaaa attccacttg ctatttaccc aagaatccac
     4561 tggaggtcat tactgccatt aaataataac tgaaaagact atgtagtgaa atgtattttt
     4621 aaaaactata ttcagtaaaa gcctgctcaa tttggagaaa tagaaccaca aacacagatc
     4681 acaggggcct tacaaagttt atgtctgaac aaataagtca attaagtaca ctttattgaa
     4741 aattgccttc cattaacaca caagaaagaa agcaggattt tctcctgtat ctgaatttta
     4801 aaattaaaaa ggcagataag acataaatag ttatcatttt aattgcaata acacagacaa
     4861 gtagttaatg atgataacaa tggtgtaact tgtaaactaa atatttggta actgaagcaa
     4921 taggcagagg aaaatagctt ttctatgaca caagtcataa gaagtccata tactgaagag
     4981 cgtttgatta aaataaagtg actattaacc agaaaagaaa cattttacat aaaatgctaa
     5041 aatttattat aggaaaataa atcaaaccca aagaaagttt attcaatgct aatttgaaag
     5101 aaaattgata agaaaacttt gagggcccaa gtccacaatt tggtgagacc actaaatttt
     5161 acatataatt atacacacac atatgtacat atatatgtat ataatcttgc ttcccgcctg
     5221 tttatggcag tactgaagag aaatgggaaa gaagagggag ggagagagaa agacgaaggg
     5281 agagagaaag cagtttccaa ggatatgttt catgtcccac cattttctca gtttctccct
     5341 ctctctccca acacacacac acacacaccc ctcacatact ataaaataaa tcttcactgc
     5401 cctatcaaaa tacaaataaa tcaatctatg ctgttctgtc cttcttgaga atctaaaaca
     5461 taccacaaaa atacatcccc agtcttttgt tctgtctgag gttagaatta attcaaattc
     5521 agaatctgtt gtgagaaatg cccaggcttt aaaaattaaa aatggatgga tcttctctga
     5581 actcagggag ggcacatact tagataccta caagacttgg aggaattaag agttcaccct
     5641 tcatctcacc aaattttccc catttttctc tttcttgtag aaggagagaa accatgctct
     5701 ctagcaacat tgagcaaaaa tcataaccac tcatctaatt tctaagaggc acctccatcg
     5761 agggccggtc tcctgcttct ttagacctct tctatctttg ttacaggaga ggacctgtgg
     5821 atagacttag ttttgacata aaacaatgcc cattcacctc ctccttcagc acaacgtcac
     5881 ccattgggca agagatccag atttgttaac aaaaaagatt ttacttcgtg attccacgtc
     5941 tataattcta tattgctaat tttttctttt gtgtgaatta ctgaatattt cagagcaaag
     6001 ctatcaactt ggagaaacag ggattaaaaa taaggataaa cactaataag agctctagaa
     6061 aaaagggaac agaaagtctg cctgtttagt aagtggcaat tccatacata ttttagagtt
     6121 ttttctatct aaaattagtt aaatacttag aatgtttgta atgagtgttc gatatttgct
     6181 ataggtttta gggttttgta aatcttcata gtaattataa acatttgtaa aatttgtaaa
     6241 atactataag tcattttgag tgttggtgtt aagcatgaaa caaacagcag ctgttgtcct
     6301 taaaaatgaa ttgacctggc cgggcgcggt ggctcacgcc tgtaatccca gcactttggg
     6361 aggccgaggc gggtggatca tgaggtcagg agatcgagac catcctggct aacaaggtga
     6421 aaccccgtct ctactaaaaa tacaaaaaat tagccgggcg cggtggcggg cgcctgtagt
     6481 cccagctact tgggaggctg aggcaggaga atggcgtgaa cccgggaagc ggagcttgca
     6541 gtgagccgag attgcgccac tgcagtccgc agtccggcct gggcgacaga gcgagactcc
     6601 gtctcaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa tgaattgacc
     6661 tgtgacacct gtagcctaat aaacatatta agaaaagtac ttagtattgt atagatattt
     6721 ggattccaag agaaaatgca acatttataa taagaagtcc atactctttt tcttacagca
     6781 gagcgtcaca ctgagttcca ttttaaaaaa ggactcattt ttcaggccaa caactgttgc
     6841 atttgaatca gatataaata atagattttc cacaaacact cagctgattg tagcagtgtt
     6901 atttaagctg gtatgtttat tttttttttc ttggaaggat agctttatat tttggctttc
     6961 attataaatt gtgttctgct tgtgtttaaa tggcttactt ataagctaga gcactatatg
     7021 ggagtgttct tctctgtatg gtatcctttt atttgcttgg ctgggttcat aacagtgtgt
     7081 gccatattct ttcttttcac tgattctaag ccatgagact tattagcatc tggtggcaag
     7141 ctgcagggac cattaacagt gtgtctcttt gactacaacg tggatctgcc actacgcgct
     7201 atgtaccatt atgtacaatc tctatttggc tcaacttcga tgaaaaagaa ataaatattt
     7261 gctaaactct caa
//
