LOCUS       NM_003006               2573 bp    mRNA    linear   PRI 01-OCT-2024
DEFINITION  Homo sapiens selectin P ligand (SELPLG), transcript variant 2,
            mRNA.
ACCESSION   NM_003006
VERSION     NM_003006.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Wang,X., Qian,J., Mi,Y., Li,Y., Cao,Y. and Qiao,K.
  TITLE     Correlations of PSGL-1 VNTR polymorphism with the susceptibility to
            severe HFMD associated with EV-71 and the immune status after
            infection
  JOURNAL   Virol J 21 (1), 187 (2024)
   PUBMED   39148126
  REMARK    GeneRIF: Correlations of PSGL-1 VNTR polymorphism with the
            susceptibility to severe HFMD associated with EV-71 and the immune
            status after infection.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   O'Dwyer,M., Kirkham-McCarthy,L., Cerreto,M., Foa,R. and Natoni,A.
  TITLE     PSGL-1 decorated with sialyl Lewisa/x promotes high affinity
            binding of myeloma cells to P-selectin but is dispensable for
            E-selectin engagement
  JOURNAL   Sci Rep 14 (1), 1756 (2024)
   PUBMED   38243063
  REMARK    GeneRIF: PSGL-1 decorated with sialyl Lewis[a/x] promotes high
            affinity binding of myeloma cells to P-selectin but is dispensable
            for E-selectin engagement.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2573)
  AUTHORS   Kauffman,K., Manfra,D., Nowakowska,D., Zafari,M., Nguyen,P.A.,
            Phennicie,R., Vollmann,E.H., O'Nuallain,B., Basinski,S.,
            Komoroski,V., Rooney,K., Culyba,E.K., Wahle,J., Ries,C., Brehm,M.,
            Sazinsky,S., Feldman,I. and Novobrantseva,T.I.
  TITLE     PSGL-1 Blockade Induces Classical Activation of Human
            Tumor-associated Macrophages
  JOURNAL   Cancer Res Commun 3 (10), 2182-2194 (2023)
   PUBMED   37819238
  REMARK    GeneRIF: PSGL-1 Blockade Induces Classical Activation of Human
            Tumor-associated Macrophages.
REFERENCE   4  (bases 1 to 2573)
  AUTHORS   Diaz-Garcia,E., Garcia-Sanchez,A., Alfaro,E., Lopez-Fernandez,C.,
            Manas,E., Cano-Pumarega,I., Lopez-Collazo,E., Garcia-Rio,F. and
            Cubillos-Zapata,C.
  TITLE     PSGL-1: a novel immune checkpoint driving T-cell dysfunction in
            obstructive sleep apnea
  JOURNAL   Front Immunol 14, 1277551 (2023)
   PUBMED   37854605
  REMARK    GeneRIF: PSGL-1: a novel immune checkpoint driving T-cell
            dysfunction in obstructive sleep apnea.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2573)
  AUTHORS   Pouyani,T. and Seed,B.
  TITLE     PSGL-1 recognition of P-selectin is controlled by a tyrosine
            sulfation consensus at the PSGL-1 amino terminus
  JOURNAL   Cell 83 (2), 333-343 (1995)
   PUBMED   7585950
REFERENCE   6  (bases 1 to 2573)
  AUTHORS   Sako,D., Comess,K.M., Barone,K.M., Camphausen,R.T., Cumming,D.A.
            and Shaw,G.D.
  TITLE     A sulfated peptide segment at the amino terminus of PSGL-1 is
            critical for P-selectin binding
  JOURNAL   Cell 83 (2), 323-331 (1995)
   PUBMED   7585949
REFERENCE   7  (bases 1 to 2573)
  AUTHORS   Wilkins,P.P., Moore,K.L., McEver,R.P. and Cummings,R.D.
  TITLE     Tyrosine sulfation of P-selectin glycoprotein ligand-1 is required
            for high affinity binding to P-selectin
  JOURNAL   J Biol Chem 270 (39), 22677-22680 (1995)
   PUBMED   7559387
REFERENCE   8  (bases 1 to 2573)
  AUTHORS   Veldman,G.M., Bean,K.M., Cumming,D.A., Eddy,R.L., Sait,S.N. and
            Shows,T.B.
  TITLE     Genomic organization and chromosomal localization of the gene
            encoding human P-selectin glycoprotein ligand
  JOURNAL   J Biol Chem 270 (27), 16470-16475 (1995)
   PUBMED   7541799
REFERENCE   9  (bases 1 to 2573)
  AUTHORS   Moore,K.L., Eaton,S.F., Lyons,D.E., Lichenstein,H.S., Cummings,R.D.
            and McEver,R.P.
  TITLE     The P-selectin glycoprotein ligand from human neutrophils displays
            sialylated, fucosylated, O-linked poly-N-acetyllactosamine
  JOURNAL   J Biol Chem 269 (37), 23318-23327 (1994)
   PUBMED   7521878
REFERENCE   10 (bases 1 to 2573)
  AUTHORS   Sako,D., Chang,X.J., Barone,K.M., Vachino,G., White,H.M., Shaw,G.,
            Veldman,G.M., Bean,K.M., Ahern,T.J., Furie,B. et al.
  TITLE     Expression cloning of a functional glycoprotein ligand for
            P-selectin
  JOURNAL   Cell 75 (6), 1179-1186 (1993)
   PUBMED   7505206
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK098315.1, AK225644.1 and
            AC008119.6.
            
            On Apr 30, 2011 this sequence version replaced NM_003006.3.
            
            Summary: This gene encodes a glycoprotein that functions as a high
            affinity counter-receptor for the cell adhesion molecules P-, E-
            and L- selectin expressed on myeloid cells and stimulated T
            lymphocytes. As such, this protein plays a critical role in
            leukocyte trafficking during inflammation by tethering of
            leukocytes to activated platelets or endothelia expressing
            selectins. This protein requires two post-translational
            modifications, tyrosine sulfation and the addition of the sialyl
            Lewis x tetrasaccharide (sLex) to its O-linked glycans, for its
            high-affinity binding activity. Aberrant expression of this gene
            and polymorphisms in this gene are associated with defects in the
            innate and adaptive immune response. Alternate splicing results in
            multiple transcript variants.[provided by RefSeq, Apr 2011].
            
            Transcript Variant: This variant (2) differs in the 5' UTR, lacks a
            portion of the 5' coding region, and initiates translation at a
            downstream start codon, compared to variant 1. The encoded isoform
            (2) has a shorter N-terminus, compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803612.74400.1,
                                           SRR1660809.130254.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000550948.2/ ENSP00000447752.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-104               AK098315.1         1-104
            105-936             AK225644.1         1-832
            937-937             AC008119.6         171623-171623       c
            938-2191            AK225644.1         834-2087
            2192-2573           AC008119.6         169987-170368       c
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q24.11"
     gene            1..2573
                     /gene="SELPLG"
                     /gene_synonym="CD162; CLA; PSGL-1; PSGL1"
                     /note="selectin P ligand"
                     /db_xref="GeneID:6404"
                     /db_xref="HGNC:HGNC:10722"
                     /db_xref="MIM:600738"
     exon            1..155
                     /gene="SELPLG"
                     /gene_synonym="CD162; CLA; PSGL-1; PSGL1"
                     /inference="alignment:Splign:2.1.0"
     exon            156..2573
                     /gene="SELPLG"
                     /gene_synonym="CD162; CLA; PSGL-1; PSGL1"
                     /inference="alignment:Splign:2.1.0"
     CDS             161..1399
                     /gene="SELPLG"
                     /gene_synonym="CD162; CLA; PSGL-1; PSGL1"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 2; P-selectin glycoprotein ligand 1; cutaneous
                     lymphocyte-associated associated antigen"
                     /codon_start=1
                     /product="P-selectin glycoprotein ligand 1 isoform 2
                     precursor"
                     /protein_id="NP_002997.2"
                     /db_xref="CCDS:CCDS31895.2"
                     /db_xref="GeneID:6404"
                     /db_xref="HGNC:HGNC:10722"
                     /db_xref="MIM:600738"
                     /translation="MPLQLLLLLILLGPGNSLQLWDTWADEAEKALGPLLARDRRQAT
                     EYEYLDYDFLPETEPPEMLRNSTDTTPLTGPGTPESTTVEPAARRSTGLDAGGAVTEL
                     TTELANMGNLSTDSAAMEIQTTQPAATEAQTTQPVPTEAQTTPLAATEAQTTRLTATE
                     AQTTPLAATEAQTTPPAATEAQTTQPTGLEAQTTAPAAMEAQTTAPAAMEAQTTPPAA
                     MEAQTTQTTAMEAQTTAPEATEAQTTQPTATEAQTTPLAAMEALSTEPSATEALSMEP
                     TTKRGLFIPFSVSSVTHKGIPMAASNLSVNYPVGAPDHISVKQCLLAILILALVATIF
                     FVCTVVLAVRLSRKGHMYPVRNYSPTEMVCISSLLPDGGEGPSATANGGLSKAKSPGL
                     TPEPREDREGDDLTLHSFLP"
     regulatory      2171..2176
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SELPLG"
                     /gene_synonym="CD162; CLA; PSGL-1; PSGL1"
                     /note="hexamer: AATAAA"
     polyA_site      2194
                     /gene="SELPLG"
                     /gene_synonym="CD162; CLA; PSGL-1; PSGL1"
                     /note="major polyA site"
ORIGIN      
        1 acacacagcc attgggggtt gctcggatcc gggactgccg cagggggtgc cacagcagtg
       61 cctggcagcg tgggctggga ccttgtcact aaagcagaga agccacttct tctgggccca
      121 cgaggcagct gtcccatgct ctgctgagca cggtggtgcc atgcctctgc aactcctcct
      181 gttgctgatc ctactgggcc ctggcaacag cttgcagctg tgggacacct gggcagatga
      241 agccgagaaa gccttgggtc ccctgcttgc ccgggaccgg agacaggcca ccgaatatga
      301 gtacctagat tatgatttcc tgccagaaac ggagcctcca gaaatgctga ggaacagcac
      361 tgacaccact cctctgactg ggcctggaac ccctgagtct accactgtgg agcctgctgc
      421 aaggcgttct actggcctgg atgcaggagg ggcagtcaca gagctgacca cggagctggc
      481 caacatgggg aacctgtcca cggattcagc agctatggag atacagacca ctcaaccagc
      541 agccacggag gcacagacca ctcaaccagt gcccacggag gcacagacca ctccactggc
      601 agccacagag gcacagacaa ctcgactgac ggccacggag gcacagacca ctccactggc
      661 agccacagag gcacagacca ctccaccagc agccacggaa gcacagacca ctcaacccac
      721 aggcctggag gcacagacca ctgcaccagc agccatggag gcacagacca ctgcaccagc
      781 agccatggaa gcacagacca ctccaccagc agccatggag gcacagacca ctcaaaccac
      841 agccatggag gcacagacca ctgcaccaga agccacggag gcacagacca ctcaacccac
      901 agccacggag gcacagacca ctccactggc agccatggag gccctgtcca cagaacccag
      961 tgccacagag gccctgtcca tggaacctac taccaaaaga ggtctgttca tacccttttc
     1021 tgtgtcctct gttactcaca agggcattcc catggcagcc agcaatttgt ccgtcaacta
     1081 cccagtgggg gccccagacc acatctctgt gaagcagtgc ctgctggcca tcctaatctt
     1141 ggcgctggtg gccactatct tcttcgtgtg cactgtggtg ctggcggtcc gcctctcccg
     1201 caagggccac atgtaccccg tgcgtaatta ctcccccacc gagatggtct gcatctcatc
     1261 cctgttgcct gatgggggtg aggggccctc tgccacagcc aatgggggcc tgtccaaggc
     1321 caagagcccg ggcctgacgc cagagcccag ggaggaccgt gagggggatg acctcaccct
     1381 gcacagcttc ctcccttagc tcactctgcc atctgttttg gcaagacccc acctccacgg
     1441 gctctcctgg gccacccctg agtgcccaga ccccattcca cagctctggg cttcctcgga
     1501 gacccctggg gatggggatc ttcagggaag gaactctggc cacccaaaca ggacaagagc
     1561 agcctggggc caagcagacg ggcaagtgga gccacctctt tcctccctcc gcggatgaag
     1621 cccagccaca tttcagccga ggtccaaggc aggaggccat ttacttgaga cagattctct
     1681 cctttttcct gtcccccatc ttctctgggt ccctctaaca tctcccatgg ctctccccgc
     1741 ttctcctggt cactggagtc tcctccccat gtacccaagg aagatggagc tcccccatcc
     1801 cacacgcact gcactgccat tgtcttttgg ttgccatggt caccaaacag gaagtggaca
     1861 ttctaaggga ggagtactga agagtgacgg acttctgagg ctgtttcctg ctgctcctct
     1921 gacttggggc agcttgggtc ttcttgggca cctctctggg aaaacccagg gtgaggttca
     1981 gcctgtgagg gctgggatgg gtttcgtggg cccaagggca gacctttctt tgggactgtg
     2041 tggaccaagg agcttccatc tagtgacaag tgacccccag ctatcgcctc ttgccttccc
     2101 ctgtggccac tttccagggt ggactctgtc ttgttcactg cagtatccca actgcaggtc
     2161 cagtgcaggc aataaatatg tgatggacaa acgatagcgg aatccttcaa ggtttcaagg
     2221 ctgtctcctt caggcagcct tcccggaatt ctccatccct cagtgcagga tgggggctgg
     2281 tcctcagctg tctgccctca gcccctggcc ccccaggaag cctctttcat gggctgttag
     2341 gttgacttca gttttgcctc ttggacaaca gggggtcttg tacatccttg ggtgaccagg
     2401 aaaagttcag gctatggggg gccaaaggga gggctgcccc ttccccacca gtgaccactt
     2461 tattccactt cctccattac ccagttttgg cccacagagt ttggtccccc ccaaacctcg
     2521 gaccaatatc cctctaaaca tcaatctatc ctcctgttaa agaaaaaaaa aaa
//
