LOCUS       NM_031454               2283 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens selenoprotein O (SELENOO), mRNA; nuclear gene for
            mitochondrial product.
ACCESSION   NM_031454
VERSION     NM_031454.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Sreelatha,A., Yee,S.S., Lopez,V.A., Park,B.C., Kinch,L.N.,
            Pilch,S., Servage,K.A., Zhang,J., Jiou,J., Karasiewicz-Urbanska,M.,
            Lobocka,M., Grishin,N.V., Orth,K., Kucharczyk,R., Pawlowski,K.,
            Tomchick,D.R. and Tagliabracci,V.S.
  TITLE     Protein AMPylation by an Evolutionarily Conserved Pseudokinase
  JOURNAL   Cell 175 (3), 809-821 (2018)
   PUBMED   30270044
REFERENCE   3  (bases 1 to 2283)
  AUTHORS   Gladyshev,V.N., Arner,E.S., Berry,M.J., Brigelius-Flohe,R.,
            Bruford,E.A., Burk,R.F., Carlson,B.A., Castellano,S., Chavatte,L.,
            Conrad,M., Copeland,P.R., Diamond,A.M., Driscoll,D.M., Ferreiro,A.,
            Flohe,L., Green,F.R., Guigo,R., Handy,D.E., Hatfield,D.L.,
            Hesketh,J., Hoffmann,P.R., Holmgren,A., Hondal,R.J., Howard,M.T.,
            Huang,K., Kim,H.Y., Kim,I.Y., Kohrle,J., Krol,A., Kryukov,G.V.,
            Lee,B.J., Lee,B.C., Lei,X.G., Liu,Q., Lescure,A., Lobanov,A.V.,
            Loscalzo,J., Maiorino,M., Mariotti,M., Sandeep Prabhu,K.,
            Rayman,M.P., Rozovsky,S., Salinas,G., Schmidt,E.E., Schomburg,L.,
            Schweizer,U., Simonovic,M., Sunde,R.A., Tsuji,P.A., Tweedie,S.,
            Ursini,F., Whanger,P.D. and Zhang,Y.
  TITLE     Selenoprotein Gene Nomenclature
  JOURNAL   J Biol Chem 291 (46), 24036-24040 (2016)
   PUBMED   27645994
REFERENCE   4  (bases 1 to 2283)
  AUTHORS   Han,S.J., Lee,B.C., Yim,S.H., Gladyshev,V.N. and Lee,S.R.
  TITLE     Characterization of mammalian selenoprotein o: a redox-active
            mitochondrial protein
  JOURNAL   PLoS One 9 (4), e95518 (2014)
   PUBMED   24751718
  REMARK    GeneRIF: SelO is a redox-active mitochondrial selenoprotein.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2283)
  AUTHORS   Dudkiewicz,M., Szczepinska,T., Grynberg,M. and Pawlowski,K.
  TITLE     A novel protein kinase-like domain in a selenoprotein, widespread
            in the tree of life
  JOURNAL   PLoS One 7 (2), e32138 (2012)
   PUBMED   22359664
REFERENCE   6  (bases 1 to 2283)
  AUTHORS   Collins,J.E., Wright,C.L., Edwards,C.A., Davis,M.P., Grinham,J.A.,
            Cole,C.G., Goward,M.E., Aguado,B., Mallya,M., Mokrab,Y.,
            Huckle,E.J., Beare,D.M. and Dunham,I.
  TITLE     A genome annotation-driven approach to cloning the human ORFeome
  JOURNAL   Genome Biol 5 (10), R84 (2004)
   PUBMED   15461802
REFERENCE   7  (bases 1 to 2283)
  AUTHORS   Kryukov,G.V., Castellano,S., Novoselov,S.V., Lobanov,A.V.,
            Zehtab,O., Guigo,R. and Gladyshev,V.N.
  TITLE     Characterization of mammalian selenoproteomes
  JOURNAL   Science 300 (5624), 1439-1443 (2003)
   PUBMED   12775843
REFERENCE   8  (bases 1 to 2283)
  AUTHORS   Dunham,I., Shimizu,N., Roe,B.A., Chissoe,S., Hunt,A.R.,
            Collins,J.E., Bruskiewich,R., Beare,D.M., Clamp,M., Smink,L.J.,
            Ainscough,R., Almeida,J.P., Babbage,A., Bagguley,C., Bailey,J.,
            Barlow,K., Bates,K.N., Beasley,O., Bird,C.P., Blakey,S.,
            Bridgeman,A.M., Buck,D., Burgess,J., Burrill,W.D., O'Brien,K.P. et
            al.
  TITLE     The DNA sequence of human chromosome 22
  JOURNAL   Nature 402 (6761), 489-495 (1999)
   PUBMED   10591208
  REMARK    Erratum:[Nature 2000 Apr 20;404(6780):904]
REFERENCE   9  (bases 1 to 2283)
  AUTHORS   Mabuchi,H., Tatami,R., Ueda,K., Ueda,R., Haba,T., Kametani,T.,
            Watanabe,A., Wakasugi,T., Ito,S., Koizumi,J., Ohta,M., Miyamoto,S.
            and Takeda,R.
  TITLE     Serum lipid and lipoprotein levels in Japanese patients with
            familial hypercholesterolemia
  JOURNAL   Atherosclerosis 32 (4), 435-444 (1979)
   PUBMED   223596
  REMARK    GeneRIF: The SELO protein has a protein domain with significant
            albeit remote sequence similarity to protein kinase-like proteins.
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AY324823.1.
            
            On Nov 22, 2018 this sequence version replaced NM_031454.1.
            
            Summary: This gene encodes a selenoprotein that is localized to the
            mitochondria. It is the largest mammalian selenoprotein, containing
            the rare amino acid selenocysteine (Sec). Sec is encoded by the UGA
            codon, which normally signals translation termination. The 3' UTRs
            of selenoprotein mRNAs contain a conserved stem-loop structure,
            designated the Sec insertion sequence (SECIS) element, that is
            necessary for the recognition of UGA as a Sec codon, rather than as
            a stop signal. The exact function of this selenoprotein is not
            known, but it is thought to have redox activity. [provided by
            RefSeq, Dec 2016].
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC110866.1, AY324823.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968189
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: PMID: 24751718
            MANE Ensembl match                 :: ENST00000380903.7/
                                                  ENSP00000370288.2
            protein contains selenocysteine    :: PMID: 12775843
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2283              AY324823.1         33-2315
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="22"
                     /map="22q13.33"
     gene            1..2283
                     /gene="SELENOO"
                     /gene_synonym="SELO"
                     /note="selenoprotein O"
                     /db_xref="GeneID:83642"
                     /db_xref="HGNC:HGNC:30395"
                     /db_xref="MIM:607917"
     exon            1..580
                     /gene="SELENOO"
                     /gene_synonym="SELO"
                     /inference="alignment:Splign:2.1.0"
     CDS             27..2036
                     /gene="SELENOO"
                     /gene_synonym="SELO"
                     /EC_number="2.7.7.108"
                     /note="UGA stop codon recoded as selenocysteine"
                     /codon_start=1
                     /transl_except=(pos:2025..2027,aa:Sec)
                     /product="protein adenylyltransferase SelO, mitochondrial"
                     /protein_id="NP_113642.1"
                     /db_xref="CCDS:CCDS43034.1"
                     /db_xref="GeneID:83642"
                     /db_xref="HGNC:HGNC:30395"
                     /db_xref="MIM:607917"
                     /translation="MAVYRAALGASLAAARLLPLGRCSPSPAPRSTLSGAAMEPAPRW
                     LAGLRFDNRALRALPVEAPPPGPEGAPSAPRPVPGACFTRVQPTPLRQPRLVALSEPA
                     LALLGLGAPPAREAEAEAALFFSGNALLPGAEPAAHCYCGHQFGQFAGQLGDGAAMYL
                     GEVCTATGERWELQLKGAGPTPFSRQADGRKVLRSSIREFLCSEAMFHLGVPTTRAGA
                     CVTSESTVVRDVFYDGNPKYEQCTVVLRVASTFIRFGSFEIFKSADEHTGRAGPSVGR
                     NDIRVQLLDYVISSFYPEIQAAHASDSVQRNAAFFREVTRRTARMVAEWQCVGFCHGV
                     LNTDNMSILGLTIDYGPFGFLDRYDPDHVCNASDNTGRYAYSKQPEVCRWNLRKLAEA
                     LQPELPLELGEAILAEEFDAEFQRHYLQKMRRKLGLVQVELEEDGALVSKLLETMHLT
                     GADFTNTFYLLSSFPVELESPGLAEFLARLMEQCASLEELRLAFRPQMDPRQLSMMLM
                     LAQSNPQLFALMGTRAGIARELERVEQQSRLEQLSAAELQSRNQGHWADWLQAYRARL
                     DKDLEGAGDAAAWQAEHVRVMHANNPKYVLRNYIAQNAIEAAERGDFSEVRRVLKLLE
                     TPYHCEAGAATDAEATEADGADGRQRSYSSKPPLWAAELCVTUSS"
     exon            581..784
                     /gene="SELENOO"
                     /gene_synonym="SELO"
                     /inference="alignment:Splign:2.1.0"
     exon            785..965
                     /gene="SELENOO"
                     /gene_synonym="SELO"
                     /inference="alignment:Splign:2.1.0"
     exon            966..1096
                     /gene="SELENOO"
                     /gene_synonym="SELO"
                     /inference="alignment:Splign:2.1.0"
     exon            1097..1377
                     /gene="SELENOO"
                     /gene_synonym="SELO"
                     /inference="alignment:Splign:2.1.0"
     exon            1378..1528
                     /gene="SELENOO"
                     /gene_synonym="SELO"
                     /inference="alignment:Splign:2.1.0"
     exon            1529..1714
                     /gene="SELENOO"
                     /gene_synonym="SELO"
                     /inference="alignment:Splign:2.1.0"
     exon            1715..1871
                     /gene="SELENOO"
                     /gene_synonym="SELO"
                     /inference="alignment:Splign:2.1.0"
     exon            1872..2283
                     /gene="SELENOO"
                     /gene_synonym="SELO"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2150..2228
                     /regulatory_class="recoding_stimulatory_region"
                     /gene="SELENOO"
                     /gene_synonym="SELO"
                     /note="SECIS_element"
                     /function="essential for recoding UGA to specify
                     selenocysteine"
     regulatory      2262..2267
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SELENOO"
                     /gene_synonym="SELO"
                     /note="hexamer: AATAAA"
     polyA_site      2283
                     /gene="SELENOO"
                     /gene_synonym="SELO"
                     /note="major polyA site"
ORIGIN      
        1 ggcttccggc gggagcgggg ccgcggatgg ccgtatacag ggcagcgctc ggggcttcgc
       61 tcgcggctgc ccgactcttg cccctcggtc gctgttcccc gtcgccggcg ccccgctcta
      121 cgttgtcggg cgccgccatg gagcccgcgc ctcgctggct ggcggggctg cgcttcgaca
      181 accgcgccct gcgcgccctg cccgtggagg cgccgccgcc cggtcccgag ggcgccccgt
      241 ccgcgccgcg gcccgtgccc ggggcctgct tcacccgcgt gcagcccacc ccgctgcggc
      301 agccgcgcct cgtggcgctg tcagagcccg cgctggcgtt gctgggcctg ggcgcgccgc
      361 ccgcgcgcga ggccgaggcc gaggccgcgc tgttcttcag cggcaacgcg ctcctgccgg
      421 gcgccgagcc cgccgcgcac tgctactgcg gccaccaatt cggccagttc gccgggcagc
      481 tgggcgacgg cgccgccatg tacctgggcg aggtgtgcac ggcgaccggc gagcgctggg
      541 agctgcagct caagggcgcc gggcccacgc ccttctccag acaggccgac ggtcgcaagg
      601 tcctacggtc aagcatccgg gagtttctat gcagcgaagc catgttccac ctgggagtcc
      661 ccaccacacg ggccggcgcc tgcgtcacgt ccgagtccac ggtggtgcgc gacgtgttct
      721 atgatggtaa tcccaaatat gaacaatgca cggttgtgtt gcgtgtagct tccactttca
      781 taaggtttgg atcctttgag atttttaagt ctgcagatga gcacacaggg cgtgcaggcc
      841 ccagcgtggg gaggaacgac attcgagtgc agctgctcga ctatgtcatc agctcctttt
      901 accccgagat ccaggctgct catgccagcg acagcgtgca gagaaatgct gccttcttcc
      961 gggaggtgac gcggcgcacg gcgcggatgg tggccgagtg gcagtgtgtg ggcttctgcc
     1021 acggcgtgct caacaccgac aacatgagca tcctggggct caccatcgac tacgggccct
     1081 ttggcttcct ggacaggtac gaccccgacc acgtgtgcaa tgcctccgac aacaccggcc
     1141 gctacgcgta cagcaagcag cccgaggtgt gcaggtggaa cctgcggaag ctggccgagg
     1201 ccctgcagcc ggaactgccc ctggagctgg gggaggccat cctggccgag gagtttgacg
     1261 ccgagttcca aaggcactac ctgcagaaga tgcgcaggaa gctgggcctc gtgcaggtgg
     1321 agctggagga agacggggcg ctggtgtcca agctcctgga gaccatgcat ctgaccggtg
     1381 ccgacttcac aaacaccttc tacttgctga gctccttccc agtggagcta gagtcgccag
     1441 gcctggcgga attcctggcc aggctgatgg agcagtgtgc ctccctggag gagctgaggc
     1501 tggccttccg gccccagatg gatccccggc agctatccat gatgctgatg ctggcgcagt
     1561 caaacccgca gctgttcgcg cttatgggca cccgggcagg catcgccagg gagctggagc
     1621 gtgtggagca gcagtctcgg ctggagcagc tgagtgcggc agagctgcag agcaggaacc
     1681 agggccactg ggctgactgg ctacaggcgt acagagcccg gctggacaag gacctggaag
     1741 gcgctgggga cgctgccgcc tggcaggctg agcacgtgcg cgtgatgcac gccaacaacc
     1801 cgaagtacgt gctgaggaac tacatcgcgc agaatgccat cgaggctgcc gagcgcgggg
     1861 acttctcaga ggtgcggcgg gtgctgaaac tactggagac cccttaccac tgcgaggcgg
     1921 gggccgccac agacgccgag gccacggaag ccgacggggc ggacggcagg cagcgctcct
     1981 acagcagtaa gcccccgctc tgggcagcag aactgtgcgt gacatgatct tcgtaacggc
     2041 ctcggcacgc tccacacccc tggagtctcc cgaggccccc atgtgctgct gagtggccaa
     2101 gatgatgcca ggctgcccta tacactgggg gattctgccc tggcccatgc acacccgtct
     2161 ttccatgatg gcagagacat ccagtcagga cctgacccgt ctctgtctga ggccggctca
     2221 gcagtgcagc ctggtccctg ggggctggac ccaggctcct aaataaacca gcaactccct
     2281 cga
//
