LOCUS       NM_033505               8066 bp    mRNA    linear   PRI 01-AUG-2024
DEFINITION  Homo sapiens selenoprotein I (SELENOI), transcript variant 1, mRNA.
ACCESSION   NM_033505 XM_371484
VERSION     NM_033505.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8066)
  AUTHORS   Li,F., Shi,Z., Cheng,M., Zhou,Z., Chu,M., Sun,L. and Zhou,J.C.
  TITLE     Biology and Roles in Diseases of Selenoprotein I Characterized by
            Ethanolamine Phosphotransferase Activity and Antioxidant Potential
  JOURNAL   J Nutr 153 (11), 3164-3172 (2023)
   PUBMED   36963501
  REMARK    GeneRIF: Biology and Roles in Diseases of Selenoprotein I
            Characterized by Ethanolamine Phosphotransferase Activity and
            Antioxidant Potential.
            Review article
REFERENCE   2  (bases 1 to 8066)
  AUTHORS   Sarma,A.S., Siddardha,B., T,P.L., Ranganath,P. and Dalal,A.
  TITLE     A novel homozygous synonymous splicing variant in SELENOI gene
            causes spastic paraplegia 81
  JOURNAL   J Gene Med 25 (7), e3501 (2023)
   PUBMED   36942482
  REMARK    GeneRIF: A novel homozygous synonymous splicing variant in SELENOI
            gene causes spastic paraplegia 81.
REFERENCE   3  (bases 1 to 8066)
  AUTHORS   Nunes,L.G.A., Pitts,M.W. and Hoffmann,P.R.
  TITLE     Selenoprotein I (selenoi) as a critical enzyme in the central
            nervous system
  JOURNAL   Arch Biochem Biophys 729, 109376 (2022)
   PUBMED   36007576
  REMARK    GeneRIF: Selenoprotein I (selenoi) as a critical enzyme in the
            central nervous system.
REFERENCE   4  (bases 1 to 8066)
  AUTHORS   Ma,C., Martinez-Rodriguez,V. and Hoffmann,P.R.
  TITLE     Roles for Selenoprotein I and Ethanolamine Phospholipid Synthesis
            in T Cell Activation
  JOURNAL   Int J Mol Sci 22 (20), 11174 (2021)
   PUBMED   34681834
  REMARK    GeneRIF: Roles for Selenoprotein I and Ethanolamine Phospholipid
            Synthesis in T Cell Activation.
            Review article
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 8066)
  AUTHORS   Varlamova,E.G., Goltyaev,M.V., Novoselov,V.I. and Fesenko,E.E.
  TITLE     Cloning, intracellular localization, and expression of the
            mammalian selenocysteine-containing protein SELENOI (SelI) in tumor
            cell lines
  JOURNAL   Dokl Biochem Biophys 476 (1), 320-322 (2017)
   PUBMED   29101747
  REMARK    GeneRIF: intracellular localization of human selenoprotein SelI and
            the degree of expression of its gene in different human tumor cell
            lines
REFERENCE   6  (bases 1 to 8066)
  AUTHORS   Ahmed,M.Y., Al-Khayat,A., Al-Murshedi,F., Al-Futaisi,A.,
            Chioza,B.A., Pedro Fernandez-Murray,J., Self,J.E., Salter,C.G.,
            Harlalka,G.V., Rawlins,L.E., Al-Zuhaibi,S., Al-Azri,F.,
            Al-Rashdi,F., Cazenave-Gassiot,A., Wenk,M.R., Al-Salmi,F.,
            Patton,M.A., Silver,D.L., Baple,E.L., McMaster,C.R. and Crosby,A.H.
  TITLE     A mutation of EPT1 (SELENOI) underlies a new disorder of Kennedy
            pathway phospholipid biosynthesis
  JOURNAL   Brain 140 (3), 547-554 (2017)
   PUBMED   28052917
  REMARK    GeneRIF: These studies define the first human disorder arising due
            to defective CDP-ethanolamine biosynthesis and provide new insight
            into the role of Kennedy pathway components in human neurological
            function
REFERENCE   7  (bases 1 to 8066)
  AUTHORS   Hasson,S.A., Kane,L.A., Yamano,K., Huang,C.H., Sliter,D.A.,
            Buehler,E., Wang,C., Heman-Ackah,S.M., Hessa,T., Guha,R.,
            Martin,S.E. and Youle,R.J.
  TITLE     High-content genome-wide RNAi screens identify regulators of parkin
            upstream of mitophagy
  JOURNAL   Nature 504 (7479), 291-295 (2013)
   PUBMED   24270810
REFERENCE   8  (bases 1 to 8066)
  AUTHORS   Oshikawa,M., Tsutsui,C., Ikegami,T., Fuchida,Y., Matsubara,M.,
            Toyama,S., Usami,R., Ohtoko,K. and Kato,S.
  TITLE     Full-length transcriptome analysis of human retina-derived cell
            lines ARPE-19 and Y79 using the vector-capping method
  JOURNAL   Invest Ophthalmol Vis Sci 52 (9), 6662-6670 (2011)
   PUBMED   21697133
  REMARK    Publication Status: Online-Only
REFERENCE   9  (bases 1 to 8066)
  AUTHORS   Horibata,Y. and Hirabayashi,Y.
  TITLE     Identification and characterization of human
            ethanolaminephosphotransferase1
  JOURNAL   J Lipid Res 48 (3), 503-508 (2007)
   PUBMED   17132865
REFERENCE   10 (bases 1 to 8066)
  AUTHORS   Kryukov,G.V., Castellano,S., Novoselov,S.V., Lobanov,A.V.,
            Zehtab,O., Guigo,R. and Gladyshev,V.N.
  TITLE     Characterization of mammalian selenoproteomes
  JOURNAL   Science 300 (5624), 1439-1443 (2003)
   PUBMED   12775843
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC010896.15.
            
            On Nov 23, 2018 this sequence version replaced NM_033505.3.
            
            Summary: The multi-pass transmembrane protein encoded by this gene
            belongs to the CDP-alcohol phosphatidyltransferase class-I family.
            It catalyzes the transfer of phosphoethanolamine from
            CDP-ethanolamine to diacylglycerol to produce
            phosphatidylethanolamine, which is involved in the formation and
            maintenance of vesicular membranes, regulation of lipid metabolism,
            and protein folding. This protein is a selenoprotein, containing
            the rare selenocysteine (Sec) amino acid at its active site. Sec is
            encoded by the UGA codon, which normally signals translation
            termination. The 3' UTRs of selenoprotein mRNAs contain a conserved
            stem-loop structure, designated the Sec insertion sequence (SECIS)
            element, that is necessary for the recognition of UGA as a Sec
            codon rather than as a stop signal. Alternatively spliced
            transcript variants have been found for this gene. [provided by
            RefSeq, Jul 2016].
            
            Transcript Variant: This variant (1) represents the
            selenoprotein-encoding transcript.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BK001426.1, AB051511.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match              :: ENST00000260585.12/
                                               ENSP00000260585.7
            protein contains selenocysteine :: PMID: 12775843
            RefSeq Select criteria          :: based on single protein-coding
                                               transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-147               AC010896.15        116482-116628
            148-216             AC010896.15        134641-134709
            217-325             AC010896.15        135171-135279
            326-400             AC010896.15        137485-137559
            401-663             AC010896.15        143706-143968
            664-772             AC010896.15        145379-145487
            773-821             AC010896.15        153638-153686
            822-1002            AC010896.15        155298-155478
            1003-1185           AC010896.15        156693-156875
            1186-8066           AC010896.15        159344-166224
FEATURES             Location/Qualifiers
     source          1..8066
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2p23.3"
     gene            1..8066
                     /gene="SELENOI"
                     /gene_synonym="EPT1; SELI; SEPI; SPG81"
                     /note="selenoprotein I"
                     /db_xref="GeneID:85465"
                     /db_xref="HGNC:HGNC:29361"
                     /db_xref="MIM:607915"
     exon            1..147
                     /gene="SELENOI"
                     /gene_synonym="EPT1; SELI; SEPI; SPG81"
                     /inference="alignment:Splign:2.1.0"
     CDS             91..1284
                     /gene="SELENOI"
                     /gene_synonym="EPT1; SELI; SEPI; SPG81"
                     /EC_number="2.7.8.1"
                     /note="UGA stop codon recoded as selenocysteine; hEPT1;
                     ethanolaminephosphotransferase 1
                     (CDP-ethanolamine-specific)"
                     /codon_start=1
                     /transl_except=(pos:1249..1251,aa:Sec)
                     /product="ethanolaminephosphotransferase 1"
                     /protein_id="NP_277040.1"
                     /db_xref="CCDS:CCDS46240.1"
                     /db_xref="GeneID:85465"
                     /db_xref="HGNC:HGNC:29361"
                     /db_xref="MIM:607915"
                     /translation="MAGYEYVSPEQLAGFDKYKYSAVDTNPLSLYVMHPFWNTIVKVF
                     PTWLAPNLITFSGFLLVVFNFLLMAYFDPDFYASAPGHKHVPDWVWIVVGILNFVAYT
                     LDGVDGKQARRTNSSTPLGELFDHGLDSWSCVYFVVTVYSIFGRGSTGVSVFVLYLLL
                     WVVLFSFILSHWEKYNTGILFLPWGYDISQVTISFVYIVTAVVGVEAWYEPFLFNFLY
                     RDLFTAMIIGCALCVTLPMSLLNFFRSYKNNTLKLNSVYEAMVPLFSPCLLFILSTAW
                     ILWSPSDILELHPRVFYFMVGTAFANSTCQLIVCQMSSTRCPTLNWLLVPLFLVVLVV
                     NLGVASYVESILLYTLTTAFTLAHIHYGVRVVKQLSSHFQIYPFSLRKPNSDULGMEE
                     KNIGL"
     exon            148..216
                     /gene="SELENOI"
                     /gene_synonym="EPT1; SELI; SEPI; SPG81"
                     /inference="alignment:Splign:2.1.0"
     exon            217..325
                     /gene="SELENOI"
                     /gene_synonym="EPT1; SELI; SEPI; SPG81"
                     /inference="alignment:Splign:2.1.0"
     exon            326..400
                     /gene="SELENOI"
                     /gene_synonym="EPT1; SELI; SEPI; SPG81"
                     /inference="alignment:Splign:2.1.0"
     exon            401..663
                     /gene="SELENOI"
                     /gene_synonym="EPT1; SELI; SEPI; SPG81"
                     /inference="alignment:Splign:2.1.0"
     exon            664..772
                     /gene="SELENOI"
                     /gene_synonym="EPT1; SELI; SEPI; SPG81"
                     /inference="alignment:Splign:2.1.0"
     exon            773..821
                     /gene="SELENOI"
                     /gene_synonym="EPT1; SELI; SEPI; SPG81"
                     /inference="alignment:Splign:2.1.0"
     exon            822..1002
                     /gene="SELENOI"
                     /gene_synonym="EPT1; SELI; SEPI; SPG81"
                     /inference="alignment:Splign:2.1.0"
     exon            1003..1185
                     /gene="SELENOI"
                     /gene_synonym="EPT1; SELI; SEPI; SPG81"
                     /inference="alignment:Splign:2.1.0"
     exon            1186..8066
                     /gene="SELENOI"
                     /gene_synonym="EPT1; SELI; SEPI; SPG81"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2549..2627
                     /regulatory_class="recoding_stimulatory_region"
                     /gene="SELENOI"
                     /gene_synonym="EPT1; SELI; SEPI; SPG81"
                     /note="SECIS_element"
                     /function="essential for recoding UGA to specify
                     selenocysteine"
     regulatory      8037..8042
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SELENOI"
                     /gene_synonym="EPT1; SELI; SEPI; SPG81"
                     /note="hexamer: AGTAAA"
     polyA_site      8066
                     /gene="SELENOI"
                     /gene_synonym="EPT1; SELI; SEPI; SPG81"
                     /note="major polyA site"
ORIGIN      
        1 gccatccttg cccagccggt gtggtgcttg tgtgtcacag ccttgtagcc gggagtcgct
       61 gccgagtggg cgctcagttt tcgggtcgtc atggctggct acgaatacgt gagcccggag
      121 cagctggctg gctttgataa gtacaagtac agtgctgtgg ataccaatcc actttctctg
      181 tatgtcatgc atccattctg gaacactata gtaaaggtat ttcctacttg gctggcgccc
      241 aatctgataa ctttttctgg ctttctgctg gtcgtattca attttctgct aatggcatac
      301 tttgatcctg acttttatgc ctcagcacca ggtcacaagc acgtgcctga ctgggtttgg
      361 attgtagtgg gcatcctcaa cttcgtagcc tacactctag atggtgtgga cggaaagcaa
      421 gctcgcagaa ccaattctag cactccctta ggggagcttt ttgatcatgg cctggatagt
      481 tggtcatgtg tttactttgt tgtgactgtt tattccatct ttggaagagg atcaactggt
      541 gtcagtgttt ttgttcttta tctcctgcta tgggtagttt tgttttcttt catcctgtcc
      601 cactgggaaa agtataacac agggattctt ttcctgccat ggggatatga cattagccag
      661 gtgactattt cttttgtcta catagtgact gcagttgtgg gagttgaggc ctggtatgaa
      721 cctttcctgt ttaatttctt atatagagac ctattcactg caatgattat tggttgtgca
      781 ttatgtgtga ctcttccaat gagtttatta aactttttca gaagctataa aaataacacc
      841 ttgaaactca attcagtcta tgaagctatg gttcccttat tttctccatg cttgctgttc
      901 attttgtcta cagcgtggat cctttggtca ccttcagata ttttagagct acatcctaga
      961 gtattctact ttatggttgg aacagctttt gccaacagta catgtcagct gattgtttgc
     1021 caaatgagta gtacccggtg tccaactttg aattggttgc tggttcctct cttcttggtt
     1081 gtcttagtgg taaacctagg agtagcctct tacgttgaga gcattctcct gtatacatta
     1141 acaactgctt ttactctggc ccacatccat tatggagtac gagtggtaaa gcagctgagc
     1201 agccattttc agatttaccc cttctcattg aggaaaccaa actcagattg actaggaatg
     1261 gaagaaaaga atattggcct gtaataatct ttctttgggc acaaagaagt actgtaaata
     1321 aatgcttgta aatatttcct ccatcaccat tgaactagac tgatctgctt gacagacgtg
     1381 ggatctcagt atggtacttg gacagcagga atgatacata taatctgaac ttgggaaatt
     1441 ttggacctac taactctaag cctattttat tttttataaa actatgtgac attttggttg
     1501 agcagaatgt acgttagacc agcaaaatgt tcctaatggt tctaacttcg tgagtttaca
     1561 atgttgtgat tcatgcaggg ttaaagatgc tttgttttta ttttttaagt accaaaattg
     1621 gtttcagaac actgataaca ctcagaaaac cacagtgtgt tttcatattt ggaactttgt
     1681 aatagcggga gtagcagtag tccaaaccta gtatagggaa aggataaaaa taagtcacct
     1741 tcaccaagag atgccaatga ttaccaaaca acagacagtt gccaaatact ggtttctctt
     1801 tcccctgaaa atggcttttg ttctcaaatg ataagagagc taatacattt agctaatatt
     1861 ctagctctct ttattatgga acagatcttg atagatggtt taattttctc ctaaagagaa
     1921 ataatcagtt gagaatttga gaatgggttg taattatcgc tcacccattg ggatggttca
     1981 ttgtttaaat atggcatttt ccccccttca gctgcaggtt cctgagattt ggtgcctgtg
     2041 agctctgatt gtaggaatgc atgtgacagt cccagtccta tggtaatgac ttaggaggaa
     2101 tgcagataaa agtaccttgt aagataaata taaattggag ttaggaattt catgaacctc
     2161 actatgacca aaattaattt tttgattcag tttgtctgtc tgtctgtcct tcccctctct
     2221 tcttttttca gggtgaggtg ctgtgtttct tatttcatac gagataaaac agagagaagt
     2281 tctctcttct ccagcttgtc catttcccca cttgaagaaa acttttgata tatatgcctt
     2341 actgagtaca tgcccccttt aatgttaaca tgacttggag taatttctga ggtttactga
     2401 caaacataaa aatcccttta attgtagtgt agttgttcta taaaccatat tttttcatga
     2461 tgtggatatt ttcttctatt tctttgtctt catttaattt ggtggtggtg aactttactt
     2521 gctgattttc ttttattttt cactgaatga agtttgtgct tgaatgaaga gtgtatctta
     2581 aacccccttt ttttggacag gctgcacttg gataaaatag gcaccactgt gttgatatgt
     2641 aattaaattc ataactattt atgaatgtga ccattgttaa atttaattat agtgtatttc
     2701 tttcttgaat ttcagtctgc tgctttgtaa aaaataactg taattgatat ttcattttta
     2761 aaatttataa attccaatca ttgtcattca agttattaga aatgagtcac ggcactaact
     2821 catggttcta tactcatttt aagaaattgt aaaggttgag gtgtatttgg tgactttctc
     2881 tgagacacag atgaacgttg gaaatagatg agaaatggat gcagaataat gcagatagtg
     2941 atcccatgtt tgttaattgc catcatggaa gtcagagaat taaccaatta agtgacggta
     3001 actatttttt gtataccttg ttctttgtga tctataaaat taaactggat aaaatgttaa
     3061 ttgggggtag ggaaaaagct ttgttttaag atttctaagc taattatttt accgttttgt
     3121 tgaccttgta cacaagaatg agattaatct gtattatata agaaaagaac tgataaatat
     3181 ttataaaatg acaaacttgg accacaggct ttcttgacct tgtacagtat agtaaaaata
     3241 ccctttactt ctgacatttt aaggtcacgt cctatagttt gggggccctt ttacttcaaa
     3301 ggtgtgtttc tgccctcttt tggttgtatc aaatttattt gaaaattgtt ttgtgctgta
     3361 ctttatgact tgtttcccta tggtggtaaa gaatgatgcc atagtcagtt ttattgacat
     3421 tgttagatga aagaaaagta gctgtcaaca aggggaaagt gaaattgagc aattcactgt
     3481 ttcaggatac ttgaggatct ttagtgtact gctgactctt tttcttgttt tgtctgccat
     3541 tcttgaaagt gcatcttgag agcccccatt tgttgtgtag agatatagca gctgaagcag
     3601 gacttcattc cagatagctg tgtgcattta tgtcatgcca attactataa catttccaaa
     3661 gtatgaaagg tctttcaggc ttatgcatga tattgtgggt gacttcatgc cccagccttt
     3721 ctccccagca tacagtgagc tgccttttgg ggagcaatgc catgataagg agcatacgtg
     3781 catgttttcc gtactttcag aatagagtgc cttagctagg ccagaggctc gtattgtgtt
     3841 tctgctgacc aaagttgcat acacctgtaa gaagtgtctt tttaggttat ggtattaaaa
     3901 aagtcgtttc aggcttatca gataataatt ctcctatatt tgaggggggc atgggataaa
     3961 aagatttaat aatacctgga tagagaatgc taatgctgcc ttttgtgatc ctttaatatt
     4021 atccatgctt ttataaagtg cagtacacat tttgacattg ccacagttta cagtgtcatt
     4081 cttccacgaa taggtaattg atagagttta tgggtcttag tcttaagggt aacagagtga
     4141 ataccttcca aataaaaagc taaatattcc tccttgtcat tttaatgtca ttattgaaat
     4201 acttttaatt ctaattttca aaagaaagct aaatgctgat tgtggtttag gatgttacag
     4261 tactaatacc atcatgttat ttattctaat atagaggcat tgagaggagg ggagtgtatg
     4321 tctagagtta ccccaattct aaataggtac caaaagagta ttgattatga cctggagcct
     4381 gagctttggg agaatataca aaggaaaaag caggcatagt ttccacttta aagggaagaa
     4441 gggactttaa catcctatga atttgaaaca tcctatgaat ttgaaagttt cctaagtgtt
     4501 gttttcaaag aactggttta ttttatagtc agtcaaaatg catttggaat gtaccttgct
     4561 tccataacag actttgactc tatgacctat gaagaataaa aagataatct gaaggtgttt
     4621 gagtcagagg ctacttttcc cctttatatt ttatatagaa gcctatttgt aattcttcct
     4681 cttcgttctt cacctgggtt tcctcttatc tacccaccac ctaggacaga ggctgcccac
     4741 tcaggtcttg ccttgttgcc cctagatgtt gccacattca catctccttg atggccttag
     4801 atctcttgac tagtcaagga ctgggaacgt gccaggggga acaaagtaag cttgatgggc
     4861 tggaactttt gactttgggt ccccaaacag ccaaaaacag atacagaaat ctttggttgt
     4921 gtggatgcct gtgttcagag tggccttatc ttgacctcag ttctggattt aagtactcta
     4981 tagaaaattt gtgaagtcag accagaattt tctttgtccc atgaggtgtc tttgtaagtc
     5041 agcatgctct tgcttgaaca ccctgtgggt gaataacttt agtaagttag ctgccagcat
     5101 tagaaccttc caggaagaat tttaactagt atgctacatt tgaagcactc tgacatgtac
     5161 atttaaagtg acagttttaa aagtaaaatg gtgttaaatc tctgaaactt gaacgtatga
     5221 tgctctggac attttaggaa gcttaagtaa atttctgatt ttcctttatt gattatgtaa
     5281 cccacgtgat ttgcatattt tagtttttat gtttgacagt ggtacaaatg actgacattg
     5341 gcaggcagtt attgtagggt atatggttaa tcacgattgt ctaacttttc cagagaaagt
     5401 ggtgatatgc ttcttccttg catggagctg tgggaattgg atttgttggc tcttcatttc
     5461 cagtgtttct acagtgcaga ataaatgtaa ctttttaagc ctgcatgcta gtaatatgct
     5521 ttgctcttta gtaaacaaat atgcaataga tagtaatcta tctacttttt ttaaagcagg
     5581 gatctgctcc ctatctagac agatctttta atgcattaat atttagagta gcaaaagaag
     5641 ttttgccatt tgtagctctc tcacaggtag cttaagcgag ggggttgtgt tgcatgtgct
     5701 tagaaaggcc aggatcccag aattggaagg tgccacatat acttttagaa tattttaaca
     5761 aaggcttgtc tctaggaaaa tccacttgga ctgcagcagg attgttctgg tcttccacag
     5821 accattcaca tggtctttct ctccagagaa tcattcttag aaagccaggc taaatcagag
     5881 tagtgctgtt tttaaagcaa gtatattgga tgatggctat agggtacctt aatgctgtgt
     5941 tgaaattgga agcagactgc tcttcagcag cacatatact gcaactgaag ttagagacag
     6001 tactgaagac gagggagtag ctaaatgctg tggatatttc atgtctgttt ggctaatgtc
     6061 ttctcttgag gagtttctat gggtagcaat taaaattaga atttatttta aggtttttat
     6121 taaaatatat acaatttgcc ataaattcta gattctgata ttcttgacaa gcactttttg
     6181 aatatctcaa gagtaagttc ttgacctgga acatatatag ttagtacttg atgtcactaa
     6241 ccaagttgta gaaggagcct gttataattc tgcaagatct gtgaatagca ttataaatct
     6301 ggggcaggtg cctgtaatcc cagcactttg ggaagccaag gcaggtggat tgcttgagcc
     6361 caggagttcg acaccaacct gagcaacatg gcgaaacccc gtctgtaata aaaatacaaa
     6421 aaaattagct gggcatggtg gcatgtgcct gtaatcccag ttactgggga ggctgaggca
     6481 ggagaatcac ttgaacctgg gagatggaga ttgcagtgag cagagattgt gccactgcac
     6541 tccagccaga gtgacagagc aagactccat ctcaaaaaat aataataaaa ataaacaaat
     6601 ctgtttaaat tactgttgcc tttcagcatt agactttgag tttaataact acaaattgag
     6661 actgctcaca atattaactt tttttgtagg ttattttgtt gtttagataa cttgcctccc
     6721 tagaggagca ttcaggagat aaagacctag ctacatgtaa tgatatgatc atttcaaaaa
     6781 tgtgccaaga aagcaaaatt cattattgaa ctctaaattt ctgggtgttt ttttttttaa
     6841 agtagcattt tctctgggta aagggaaagg caatgaatga ttgccaacat gtaaactccc
     6901 tgctgcccgc cttcccccag tcccctccat ctaacataat acagtaaatt tgtagccagt
     6961 tgtagaaaaa gaaattgata tctttctgag taaggtttca tgccctgtga ctaagaatag
     7021 gtggaggaat catggccaaa tcaaatatga attgtttagc ttggtcctgt tgcagttggc
     7081 tttctgtagt gttctgaaac aagaggacga ttccattcct tctcaggaac ctagaaacaa
     7141 caccgcttga gcaactggaa tatgtttgct gcaagcagaa tattttgggg agaggaagag
     7201 tagtttaatt caagtagttt aattgaacat attagtcatt ggtctgtctg ggacgtgcag
     7261 tgttcatagt agcaatgtat gtaccattta ttttatctgg ttgtgtgtga tgtgtgtgta
     7321 tgcctattta atatgtacac atatattcac tacatatgta tgtatgatat attcatatat
     7381 acatgcagtc tgcttgatta tcagcaaaat ggtcagcctt tatcagatag tttcttcatg
     7441 tggagttcat ctgcatgtgg cccttactct gaagcctctt cctgatctgg agccacagtc
     7501 tgtctgtctt ccagttcatc tcagtcctcg agaaaggccc tttaaatatg tcactttccc
     7561 attttccttt aaccatgggt tgtgtgagcc agaaagagct ttgagaaaga tggctgcttc
     7621 caccagggtg gaggcttcta ggtctgcatg atgatggggc ccgtttctgg ccagagggtg
     7681 gctctgggag cagttgtgct gcgggcttgc tgggggagaa ctctaactgt tgcagaaaca
     7741 gagcttcatg gcttgcttaa attacttagc tggaatattt taaagtgtca gataatgtga
     7801 tgtacaaaga gagtatgccg atgcatttca ttgtttttgc attatctgta tcagtgtttc
     7861 ccattgctag cccgaataat caagaccaga ctgtttcttg tcactggatt ctggaagcct
     7921 tgccctttca actggattta tgtttgtttc tgctgctgtg aaaccccaaa gtaatgcagt
     7981 aggttttaat tgaaaattgt ttaattctat tattgataat tatttattgt aataaaagta
     8041 aaagagtaat ttttaaatcc ttttaa
//
