LOCUS       NM_170746               1192 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens selenoprotein H (SELENOH), transcript variant 1, mRNA.
ACCESSION   NM_170746
VERSION     NM_170746.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Cao,L., Pechan,T., Lee,S. and Cheng,W.H.
  TITLE     Identification of Selenoprotein H Isoforms and Impact of
            Selenoprotein H Overexpression on Protein But Not mRNA Levels of 2
            Other Selenoproteins in 293T Cells
  JOURNAL   J Nutr 151 (11), 3329-3338 (2021)
   PUBMED   34510207
  REMARK    GeneRIF: Identification of Selenoprotein H Isoforms and Impact of
            Selenoprotein H Overexpression on Protein But Not mRNA Levels of 2
            Other Selenoproteins in 293T Cells.
            Erratum:[J Nutr. 2021 Nov 2;151(11):3597. doi: 10.1093/jn/nxab346..
            PMID: 34726735]
REFERENCE   2  (bases 1 to 1192)
  AUTHORS   Bertz,M., Kuhn,K., Koeberle,S.C., Muller,M.F., Hoelzer,D.,
            Thies,K., Deubel,S., Thierbach,R. and Kipp,A.P.
  TITLE     Selenoprotein H controls cell cycle progression and proliferation
            of human colorectal cancer cells
  JOURNAL   Free Radic Biol Med 127, 98-107 (2018)
   PUBMED   29330096
  REMARK    GeneRIF: The high levels of SELENOH in tumors as well as in
            undifferentiated, proliferative cells together with its inhibitory
            effects on proliferation and G1/S phase transition suggest SELENOH
            as a key regulator for cell cycle progression and for prevention of
            uncontrolled proliferation
REFERENCE   3  (bases 1 to 1192)
  AUTHORS   Barage,S.H., Deobagkar,D.D. and Baladhye,V.B.
  TITLE     Characterization of structural and functional role of
            selenocysteine in selenoprotein H and its impact on DNA binding
  JOURNAL   Amino Acids 50 (5), 593-607 (2018)
   PUBMED   29480333
  REMARK    GeneRIF: the obtained results clearly illustrate Sec residue plays
            an important role to restore functionally active conformation of
            SELENOH. The present study broadened our current understanding
            regarding the role of selenocysteine in protein structure and
            function.
REFERENCE   4  (bases 1 to 1192)
  AUTHORS   Cox,A.G., Tsomides,A., Kim,A.J., Saunders,D., Hwang,K.L.,
            Evason,K.J., Heidel,J., Brown,K.K., Yuan,M., Lien,E.C., Lee,B.C.,
            Nissim,S., Dickinson,B., Chhangawala,S., Chang,C.J., Asara,J.M.,
            Houvras,Y., Gladyshev,V.N. and Goessling,W.
  TITLE     Selenoprotein H is an essential regulator of redox homeostasis that
            cooperates with p53 in development and tumorigenesis
  JOURNAL   Proc Natl Acad Sci U S A 113 (38), E5562-E5571 (2016)
   PUBMED   27588899
  REMARK    GeneRIF: Transcriptome analysis revealed that SepH deficiency
            induces an inflammatory response and activates the p53 pathway.
            Consequently, loss of seph renders larvae susceptible to oxidative
            stress and DNA damage. Finally, we demonstrate that seph interacts
            with p53 deficiency in adulthood to accelerate gastrointestinal
            tumor development
REFERENCE   5  (bases 1 to 1192)
  AUTHORS   Mendelev,N., Mehta,S.L., Witherspoon,S., He,Q., Sexton,J.Z. and
            Li,P.A.
  TITLE     Upregulation of human selenoprotein H in murine hippocampal
            neuronal cells promotes mitochondrial biogenesis and functional
            performance
  JOURNAL   Mitochondrion 11 (1), 76-82 (2011)
   PUBMED   20656065
  REMARK    GeneRIF: Overexpression of SelH promotes mitochondrial biogenesis
            and improves mitochondrial functional performance.
REFERENCE   6  (bases 1 to 1192)
  AUTHORS   Mendelev,N., Witherspoon,S. and Li,P.A.
  TITLE     Overexpression of human selenoprotein H in neuronal cells
            ameliorates ultraviolet irradiation-induced damage by modulating
            cell signaling pathways
  JOURNAL   Exp Neurol 220 (2), 328-334 (2009)
   PUBMED   19766117
  REMARK    GeneRIF: t SelH protects neurons against UVB-induced damage by
            inhibiting apoptotic cell death pathways
REFERENCE   7  (bases 1 to 1192)
  AUTHORS   Panee,J., Stoytcheva,Z.R., Liu,W. and Berry,M.J.
  TITLE     Selenoprotein H is a redox-sensing high mobility group family
            DNA-binding protein that up-regulates genes involved in glutathione
            synthesis and phase II detoxification
  JOURNAL   J Biol Chem 282 (33), 23759-23765 (2007)
   PUBMED   17526492
  REMARK    GeneRIF: selenoprotein H is a redox-responsive DNA-binding protein
            of the AT-hook family and functions in regulating expression levels
            of genes involved in de novo glutathione synthesis and phase II
            detoxification in response to redox status.
REFERENCE   8  (bases 1 to 1192)
  AUTHORS   Novoselov,S.V., Kryukov,G.V., Xu,X.M., Carlson,B.A., Hatfield,D.L.
            and Gladyshev,V.N.
  TITLE     Selenoprotein H is a nucleolar thioredoxin-like protein with a
            unique expression pattern
  JOURNAL   J Biol Chem 282 (16), 11960-11968 (2007)
   PUBMED   17337453
  REMARK    GeneRIF: SelH is a novel nucleolar oxidoreductase that may have
            functions which are regulated by redox and dependent on the trace
            element selenium
REFERENCE   9  (bases 1 to 1192)
  AUTHORS   Ben Jilani,K.E., Panee,J., He,Q., Berry,M.J. and Li,P.A.
  TITLE     Overexpression of selenoprotein H reduces Ht22 neuronal cell death
            after UVB irradiation by preventing superoxide formation
  JOURNAL   Int J Biol Sci 3 (4), 198-204 (2007)
   PUBMED   17389926
  REMARK    GeneRIF: hSelH overexpression protects cells from UVB
            irradiation-induced murine hippocampal neuronal cell death by
            reducing the superoxide formation.
            Publication Status: Online-Only
REFERENCE   10 (bases 1 to 1192)
  AUTHORS   Kryukov,G.V., Castellano,S., Novoselov,S.V., Lobanov,A.V.,
            Zehtab,O., Guigo,R. and Gladyshev,V.N.
  TITLE     Characterization of mammalian selenoproteomes
  JOURNAL   Science 300 (5624), 1439-1443 (2003)
   PUBMED   12775843
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AF536829.1 and AF085883.1.
            
            On Nov 22, 2018 this sequence version replaced NM_170746.3.
            
            Summary: This gene encodes a nucleolar protein, which belongs to
            the SelWTH family. It functions as an oxidoreductase, and has been
            shown to protect neurons against UVB-induced damage by inhibiting
            apoptotic cell death pathways, promote mitochondrial biogenesis and
            mitochondrial function, and suppress cellular senescence through
            genome maintenance and redox regulation. This protein is a
            selenoprotein, containing the rare amino acid selenocysteine (Sec)
            at its active site. Sec is encoded by the UGA codon, which normally
            signals translation termination. The 3' UTRs of selenoprotein mRNAs
            contain a conserved stem-loop structure, designated the Sec
            insertion sequence (SECIS) element, that is necessary for the
            recognition of UGA as a Sec codon, rather than as a stop signal.
            Alternatively spliced transcript variants have been found for this
            gene. [provided by RefSeq, May 2016].
            
            Transcript Variant: This variant (1) represents the longer
            transcript. Variants 1 and 2 encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1163655.617179.1,
                                           SRR1163655.287763.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match              :: ENST00000534355.6/
                                               ENSP00000434511.1
            protein contains selenocysteine :: PMID: 12775843
            RefSeq Select criteria          :: based on conservation,
                                               expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-680               AF536829.1         138-817
            681-1192            AF085883.1         8-519
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q12.1"
     gene            1..1192
                     /gene="SELENOH"
                     /gene_synonym="C11orf31; C17orf10; SELH"
                     /note="selenoprotein H"
                     /db_xref="GeneID:280636"
                     /db_xref="HGNC:HGNC:18251"
                     /db_xref="MIM:607914"
     exon            1..227
                     /gene="SELENOH"
                     /gene_synonym="C11orf31; C17orf10; SELH"
                     /inference="alignment:Splign:2.1.0"
     CDS             106..474
                     /gene="SELENOH"
                     /gene_synonym="C11orf31; C17orf10; SELH"
                     /note="UGA stop codon recoded as selenocysteine"
                     /codon_start=1
                     /transl_except=(pos:235..237,aa:Sec)
                     /product="selenoprotein H"
                     /protein_id="NP_734467.1"
                     /db_xref="CCDS:CCDS44602.1"
                     /db_xref="GeneID:280636"
                     /db_xref="HGNC:HGNC:18251"
                     /db_xref="MIM:607914"
                     /translation="MAPRGRKRKAEAAVVAVAEKREKLANGGEGMEEATVVIEHCTSU
                     RVYGRNAAALSQALRLEAPELPVKVNPTKPRRGSFEVTLLRPDGSSAELWTGIKKGPP
                     RKLKFPEPQEVVEELKKYLS"
     exon            228..373
                     /gene="SELENOH"
                     /gene_synonym="C11orf31; C17orf10; SELH"
                     /inference="alignment:Splign:2.1.0"
     exon            374..504
                     /gene="SELENOH"
                     /gene_synonym="C11orf31; C17orf10; SELH"
                     /inference="alignment:Splign:2.1.0"
     exon            505..1192
                     /gene="SELENOH"
                     /gene_synonym="C11orf31; C17orf10; SELH"
                     /inference="alignment:Splign:2.1.0"
     regulatory      523..590
                     /regulatory_class="recoding_stimulatory_region"
                     /gene="SELENOH"
                     /gene_synonym="C11orf31; C17orf10; SELH"
                     /note="SECIS_element"
                     /function="essential for recoding UGA to specify
                     selenocysteine"
     regulatory      660..665
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SELENOH"
                     /gene_synonym="C11orf31; C17orf10; SELH"
                     /note="hexamer: AATAAA"
     regulatory      672..677
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SELENOH"
                     /gene_synonym="C11orf31; C17orf10; SELH"
                     /note="hexamer: AATAAA"
     polyA_site      673
                     /gene="SELENOH"
                     /gene_synonym="C11orf31; C17orf10; SELH"
     polyA_site      680
                     /gene="SELENOH"
                     /gene_synonym="C11orf31; C17orf10; SELH"
                     /note="major polyA site"
     polyA_site      699
                     /gene="SELENOH"
                     /gene_synonym="C11orf31; C17orf10; SELH"
     regulatory      1172..1177
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SELENOH"
                     /gene_synonym="C11orf31; C17orf10; SELH"
                     /note="hexamer: AATAAT"
     polyA_site      1192
                     /gene="SELENOH"
                     /gene_synonym="C11orf31; C17orf10; SELH"
ORIGIN      
        1 agagcttccg ggctgcgctc ttcgttgccc agtttccgct cagtggtcgc gtctccgccc
       61 cccacccacc agtcccgctg cattctcggc cgggctctag gcgccatggc tccccgcggg
      121 aggaagcgta aggctgaggc cgcggtggtc gccgtagccg agaagcgaga gaagctggcg
      181 aacggcgggg agggaatgga ggaggcgacc gttgttatcg agcattgcac tagctgacgc
      241 gtctatgggc gcaacgccgc ggccctgagc caggcgctgc gcctggaggc cccagagctt
      301 ccagtaaagg tgaacccgac gaagccccgg aggggcagct tcgaggtgac gctgctgcgc
      361 ccggacggca gcagtgcgga gctctggact gggattaaga aggggccccc acgcaaactc
      421 aaattccctg agcctcaaga ggtggtggaa gagttgaaga agtacctgtc gtagggagat
      481 ttgggtagaa gccctcatgc tgagctttgt gtccctggtg atgttggaac attaatgatg
      541 gaacatggcc aaacttcagt catgatcctg aagccatggt ttcttccctg ccagaaatga
      601 aggttcagtt atgaggcaac cctctagtaa ggcattgtaa aagttactgg atttggttta
      661 ataaaagttg aaataaagta tttgagtaat agagtaaaac ttaatcttaa gggatggaca
      721 aagccagcct tgtggagttg gtagtccttg tgtttttcat agcccaagat agccatgtga
      781 cctggaactt ttaaaaaaaa attttttttt aagagccaga gtctcacttt gtcacccatg
      841 ctggagtata gtggtgctat ctcggctcac tgcaaccatt gtctcccagg ttcaagcgat
      901 actcctgcct cagtctcctg agtagctggg atcacaggtg cacaccacca cgcctggcta
      961 atttttttat ttttattttt agtaaaggcg gggtttcacc atgttggcca ggctggtctc
     1021 aaactcctga cctcaagtga tatgcctgcc ttgacctccc aaagtgctgg ggttacaggc
     1081 atgatgagcc accgcacctg gcctgacctg caacttttat gatctctggt aaaacatgag
     1141 aaatacttgt aaaaattatg ggagcaaagt aaataattta ttttttaaaa aa
//
