LOCUS       NM_004261               1542 bp    mRNA    linear   PRI 12-AUG-2024
DEFINITION  Homo sapiens selenoprotein F (SELENOF), transcript variant 1, mRNA.
ACCESSION   NM_004261
VERSION     NM_004261.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Flowers,B., Bochnacka,O., Poles,A., Diamond,A.M. and Kastrati,I.
  TITLE     Distinct Roles of SELENOF in Different Human Cancers
  JOURNAL   Biomolecules 13 (3), 486 (2023)
   PUBMED   36979420
  REMARK    GeneRIF: Distinct Roles of SELENOF in Different Human Cancers.
            Review article
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Bera,S. and Diamond,A.M.
  TITLE     Role of SELENBP1 and SELENOF in prostate cancer bioenergetics
  JOURNAL   Arch Biochem Biophys 732, 109451 (2022)
   PUBMED   36334799
  REMARK    GeneRIF: Role of SELENBP1 and SELENOF in prostate cancer
            bioenergetics.
            Review article
REFERENCE   3  (bases 1 to 1542)
  AUTHORS   Zhao,Z., Mousa,R. and Metanis,N.
  TITLE     One-Pot Chemical Protein Synthesis Utilizing Fmoc-Masked
            Selenazolidine to Address the Redox Functionality of Human
            Selenoprotein F
  JOURNAL   Chemistry 28 (16), e202200279 (2022)
   PUBMED   35112407
  REMARK    GeneRIF: One-Pot Chemical Protein Synthesis Utilizing Fmoc-Masked
            Selenazolidine to Address the Redox Functionality of Human
            Selenoprotein F.
REFERENCE   4  (bases 1 to 1542)
  AUTHORS   Zigrossi,A., Hong,L.K., Ekyalongo,R.C., Cruz-Alvarez,C.,
            Gornick,E., Diamond,A.M. and Kastrati,I.
  TITLE     SELENOF is a new tumor suppressor in breast cancer
  JOURNAL   Oncogene 41 (9), 1263-1268 (2022)
   PUBMED   35082382
  REMARK    GeneRIF: SELENOF is a new tumor suppressor in breast cancer.
REFERENCE   5  (bases 1 to 1542)
  AUTHORS   Gevaert,K., Goethals,M., Martens,L., Van Damme,J., Staes,A.,
            Thomas,G.R. and Vandekerckhove,J.
  TITLE     Exploring proteomes and analyzing protein processing by mass
            spectrometric identification of sorted N-terminal peptides
  JOURNAL   Nat Biotechnol 21 (5), 566-569 (2003)
   PUBMED   12665801
REFERENCE   6  (bases 1 to 1542)
  AUTHORS   Wu,H.J., Lin,C., Zha,Y.Y., Yang,J.G., Zhang,M.C., Zhang,X.Y.,
            Liang,X., Fu,M. and Wu,M.
  TITLE     [Redox reactions of Sep15 and its relationship with tumor
            development]
  JOURNAL   Ai Zheng 22 (2), 119-122 (2003)
   PUBMED   12600282
  REMARK    GeneRIF: Studies in mouse show that Sep15 may have redox function.
REFERENCE   7  (bases 1 to 1542)
  AUTHORS   Kumaraswamy,E., Korotkov,K.V., Diamond,A.M., Gladyshev,V.N. and
            Hatfield,D.L.
  TITLE     Genetic and functional analysis of mammalian Sep15 selenoprotein
  JOURNAL   Methods Enzymol 347, 187-197 (2002)
   PUBMED   11898406
  REMARK    GeneRIF: Genetic and functional analysis of mammalian Sep15
            selenoprotein.
REFERENCE   8  (bases 1 to 1542)
  AUTHORS   Korotkov,K.V., Kumaraswamy,E., Zhou,Y., Hatfield,D.L. and
            Gladyshev,V.N.
  TITLE     Association between the 15-kDa selenoprotein and
            UDP-glucose:glycoprotein glucosyltransferase in the endoplasmic
            reticulum of mammalian cells
  JOURNAL   J Biol Chem 276 (18), 15330-15336 (2001)
   PUBMED   11278576
REFERENCE   9  (bases 1 to 1542)
  AUTHORS   Kumaraswamy,E., Malykh,A., Korotkov,K.V., Kozyavkin,S., Hu,Y.,
            Kwon,S.Y., Moustafa,M.E., Carlson,B.A., Berry,M.J., Lee,B.J.,
            Hatfield,D.L., Diamond,A.M. and Gladyshev,V.N.
  TITLE     Structure-expression relationships of the 15-kDa selenoprotein
            gene. Possible role of the protein in cancer etiology
  JOURNAL   J Biol Chem 275 (45), 35540-35547 (2000)
   PUBMED   10945981
REFERENCE   10 (bases 1 to 1542)
  AUTHORS   Gladyshev,V.N., Jeang,K.T., Wootton,J.C. and Hatfield,D.L.
  TITLE     A new human selenium-containing protein. Purification,
            characterization, and cDNA sequence
  JOURNAL   J Biol Chem 273 (15), 8910-8915 (1998)
   PUBMED   9535873
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BU941543.1, BC016359.1 and
            AK225640.1.
            
            On Nov 22, 2018 this sequence version replaced NM_004261.4.
            
            Summary: The protein encoded by this gene belongs to the
            SEP15/selenoprotein M family. The exact function of this protein is
            not known; however, it has been found to associate with
            UDP-glucose:glycoprotein glucosyltransferase (UGTR), an endoplasmic
            reticulum(ER)-resident protein, which is involved in the quality
            control of protein folding. The association with UGTR retains this
            protein in the ER, where it may play a role in protein folding. It
            has also been suggested to have a role in cancer etiology. This
            protein is a selenoprotein, containing the rare amino acid
            selenocysteine (Sec). Sec is encoded by the UGA codon, which
            normally signals translation termination. The 3' UTRs of
            selenoprotein mRNAs contain a conserved stem-loop structure,
            designated the Sec insertion sequence (SECIS) element, that is
            necessary for the recognition of UGA as a Sec codon, rather than as
            a stop signal. Alternatively spliced transcript variants have been
            found for this gene. [provided by RefSeq, Nov 2016].
            
            Transcript Variant: This variant (1) represents the predominant
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660809.84236.1,
                                           SRR3476690.1166565.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2467144 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match              :: ENST00000331835.10/
                                               ENSP00000328729.6
            protein contains selenocysteine :: PMID: 9535873, 10945981
            RefSeq Select criteria          :: based on conservation,
                                               expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-10                BU941543.1         243-252
            11-1538             BC016359.1         4-1531
            1539-1542           AK225640.1         1527-1530
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p22.3"
     gene            1..1542
                     /gene="SELENOF"
                     /gene_synonym="SEP15"
                     /note="selenoprotein F"
                     /db_xref="GeneID:9403"
                     /db_xref="HGNC:HGNC:17705"
                     /db_xref="MIM:606254"
     exon            1..99
                     /gene="SELENOF"
                     /gene_synonym="SEP15"
                     /inference="alignment:Splign:2.1.0"
     CDS             16..513
                     /gene="SELENOF"
                     /gene_synonym="SEP15"
                     /note="UGA stop codon recoded as selenocysteine; isoform 1
                     precursor is encoded by transcript variant 1; 15 kDa
                     selenoprotein"
                     /codon_start=1
                     /transl_except=(pos:301..303,aa:Sec)
                     /product="selenoprotein F isoform 1 precursor"
                     /protein_id="NP_004252.2"
                     /db_xref="CCDS:CCDS76178.1"
                     /db_xref="GeneID:9403"
                     /db_xref="HGNC:HGNC:17705"
                     /db_xref="MIM:606254"
                     /translation="MVAMAAGPSGCLVPAFGLRLLLATVLQAVSAFGAEFSSEACREL
                     GFSSNLLCSSCDLLGQFNLLQLDPDCRGCCQEEAQFETKKLYAGAILEVCGUKLGRFP
                     QVQAFVRSDKPKLFRGLQIKYVRGSDPVLKLLDDNGNIAEELSILKWNTDSVEEFLSE
                     KLERI"
     exon            100..267
                     /gene="SELENOF"
                     /gene_synonym="SEP15"
                     /inference="alignment:Splign:2.1.0"
     exon            268..331
                     /gene="SELENOF"
                     /gene_synonym="SEP15"
                     /inference="alignment:Splign:2.1.0"
     exon            332..381
                     /gene="SELENOF"
                     /gene_synonym="SEP15"
                     /inference="alignment:Splign:2.1.0"
     exon            382..1542
                     /gene="SELENOF"
                     /gene_synonym="SEP15"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1103..1184
                     /regulatory_class="recoding_stimulatory_region"
                     /gene="SELENOF"
                     /gene_synonym="SEP15"
                     /note="SECIS_element"
                     /function="essential for recoding UGA to specify
                     selenocysteine"
     regulatory      1245..1250
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SELENOF"
                     /gene_synonym="SEP15"
                     /note="hexamer: AATAAA"
     polyA_site      1266
                     /gene="SELENOF"
                     /gene_synonym="SEP15"
                     /note="major polyA site"
     regulatory      1517..1522
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SELENOF"
                     /gene_synonym="SEP15"
                     /note="hexamer: ATTAAA"
     polyA_site      1542
                     /gene="SELENOF"
                     /gene_synonym="SEP15"
                     /note="major polyA site"
ORIGIN      
        1 agaaacctgc ggaaaatggt agcgatggcg gctgggccga gtgggtgtct ggtgccggcg
       61 tttgggctac ggttgttgtt ggcgactgtg cttcaagcgg tgtctgcttt tggggcagag
      121 ttttcatcgg aggcatgcag agagttaggc ttttctagca acttgctttg cagctcttgt
      181 gatcttctcg gacagttcaa cctgcttcag ctggatcctg attgcagagg atgctgtcag
      241 gaggaagcac aatttgaaac caaaaagctg tatgcaggag ctattcttga agtttgtgga
      301 tgaaaattgg gaaggttccc tcaagtccaa gcttttgtta ggagtgataa acccaaactg
      361 ttcagaggac tgcaaatcaa gtatgtccgt ggttcagacc ctgtattaaa gcttttggac
      421 gacaatggga acattgctga agaactgagc attctcaaat ggaacacaga cagtgtagaa
      481 gaattcctga gtgaaaagtt ggaacgcata taaatcttgc ttaaattttg tcctatcctt
      541 ttgttacctt atcaaatgaa atattacagc acctagaaaa taatttagtt ttgcttgctt
      601 ccattgatca gtcttttact tgaggcatta aatatctaat taaatcgtga aatggcagta
      661 tagtccatga tatctaagga gttggcaagc ttaacaaaac ccatttttta taaatgtcca
      721 tcctcctgca tttgttgata ccactaacaa aatgctttgt aacagacttg cggttaatta
      781 tgcaaatgat agtttgtgat aattggtcca gttttacgaa caacagattt ctaaattaga
      841 gaggttaaca agacagatga ttactatgcc tcatgtgctg tgtgctcttt gaaaggaatg
      901 acagcagact acaaagcaaa taagatatac tgagcctcaa cagattgcct gctcctcaga
      961 gtctctccta tttttgtatt acccagcttt ctttttaata caaatgttat ttatagttta
     1021 caatgaatgc actgcataaa aactttgtag cttcattatt gtaaaacata ttcaagatcc
     1081 tacagtaaga gtgaaacatt cacaaagatt tgcgttaatg aagactacac agaaaacctt
     1141 tctagggatt tgtgtggatc agatacatac ttggcaaatt tttgagtttt acattcttac
     1201 agaaaagtcc atttaaaagt gatcatttgt aagaccaaaa tataaataaa aagtttcaaa
     1261 aatctatctg aatttggaat tcttctggtt tgttctttca tgtttaaaaa tgatgttttt
     1321 caatgcattt ttttcatgta agcccttttt ttagccaaaa tgtaaaaatg gctgtaatat
     1381 ttaaaactta taacatctta ttgttggtaa tagtgcttta tatttgtctg attttatttt
     1441 tcaaagtttt ttcatttatg aacacatttt cattggtata ttatttaagg aatatctctt
     1501 gatatagaat ttttatatta aaaatgattt ttctttgctt aa
//
