LOCUS       NM_018144               2495 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens SEC61 translocon subunit alpha 2 (SEC61A2), transcript
            variant 1, mRNA.
ACCESSION   NM_018144
VERSION     NM_018144.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Fasci,D., van Ingen,H., Scheltema,R.A. and Heck,A.J.R.
  TITLE     Histone Interaction Landscapes Visualized by Crosslinking Mass
            Spectrometry in Intact Cell Nuclei
  JOURNAL   Mol Cell Proteomics 17 (10), 2018-2033 (2018)
   PUBMED   30021884
REFERENCE   3  (bases 1 to 2495)
  AUTHORS   Daniel,C.J., Conti,B., Johnson,A.E. and Skach,W.R.
  TITLE     Control of translocation through the Sec61 translocon by nascent
            polypeptide structure within the ribosome
  JOURNAL   J Biol Chem 283 (30), 20864-20873 (2008)
   PUBMED   18480044
REFERENCE   4  (bases 1 to 2495)
  AUTHORS   Grupe,A., Li,Y., Rowland,C., Nowotny,P., Hinrichs,A.L., Smemo,S.,
            Kauwe,J.S., Maxwell,T.J., Cherny,S., Doil,L., Tacey,K., van
            Luchene,R., Myers,A., Wavrant-De Vrieze,F., Kaleem,M.,
            Hollingworth,P., Jehu,L., Foy,C., Archer,N., Hamilton,G.,
            Holmans,P., Morris,C.M., Catanese,J., Sninsky,J., White,T.J.,
            Powell,J., Hardy,J., O'Donovan,M., Lovestone,S., Jones,L.,
            Morris,J.C., Thal,L., Owen,M., Williams,J. and Goate,A.
  TITLE     A scan of chromosome 10 identifies a novel locus showing strong
            association with late-onset Alzheimer disease
  JOURNAL   Am J Hum Genet 78 (1), 78-88 (2006)
   PUBMED   16385451
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   5  (bases 1 to 2495)
  AUTHORS   Greenfield,J.J. and High,S.
  TITLE     The Sec61 complex is located in both the ER and the ER-Golgi
            intermediate compartment
  JOURNAL   J Cell Sci 112 (Pt 10), 1477-1486 (1999)
   PUBMED   10212142
REFERENCE   6  (bases 1 to 2495)
  AUTHORS   Chen,Y., Le Caherec,F. and Chuck,S.L.
  TITLE     Calnexin and other factors that alter translocation affect the
            rapid binding of ubiquitin to apoB in the Sec61 complex
  JOURNAL   J Biol Chem 273 (19), 11887-11894 (1998)
   PUBMED   9565615
REFERENCE   7  (bases 1 to 2495)
  AUTHORS   Dodson,G. and Steiner,D.
  TITLE     The role of assembly in insulin's biosynthesis
  JOURNAL   Curr Opin Struct Biol 8 (2), 189-194 (1998)
   PUBMED   9631292
  REMARK    Review article
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BP212860.1, BC026179.1,
            AK292295.1, AC073160.8 and BX645143.1.
            
            On Nov 23, 2018 this sequence version replaced NM_018144.3.
            
            Summary: The protein encoded by this gene has similarity to a mouse
            protein which suggests a role in the insertion of secretory and
            membrane polypeptides into the endoplasmic reticulum. It may also
            be required for the assembly of membrane and secretory proteins.
            Alternative splicing results in multiple transcript variants.
            [provided by RefSeq, Dec 2008].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (a).
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803613.136380.1,
                                           SRR1660805.246684.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968189
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000298428.14/ ENSP00000298428.9
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-17                BP212860.1         37-53
            18-65               BC026179.1         4-51
            66-1829             AK292295.1         52-1815
            1830-2434           AC073160.8         161624-162228
            2435-2492           BX645143.1         21-78               c
            2493-2495           AC073160.8         162287-162289
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10p14"
     gene            1..2495
                     /gene="SEC61A2"
                     /note="SEC61 translocon subunit alpha 2"
                     /db_xref="GeneID:55176"
                     /db_xref="HGNC:HGNC:17702"
                     /db_xref="MIM:618271"
     exon            1..118
                     /gene="SEC61A2"
                     /inference="alignment:Splign:2.1.0"
     CDS             112..1542
                     /gene="SEC61A2"
                     /note="isoform a is encoded by transcript variant 1; Sec61
                     alpha form 2; protein transport protein Sec61 subunit
                     alpha; sec61 alpha-2; Sec61 alpha 2 subunit; SEC61
                     translocon alpha 2 subunit"
                     /codon_start=1
                     /product="protein transport protein Sec61 subunit alpha
                     isoform 2 isoform a"
                     /protein_id="NP_060614.2"
                     /db_xref="CCDS:CCDS7088.1"
                     /db_xref="GeneID:55176"
                     /db_xref="HGNC:HGNC:17702"
                     /db_xref="MIM:618271"
                     /translation="MGIKFLEVIKPFCAVLPEIQKPERKIQFREKVLWTAITLFIFLV
                     CCQIPLFGIMSSDSADPFYWMRVILASNRGTLMELGISPIVTSGLIMQLLAGAKIIEV
                     GDTPKDRALFNGAQKLFGMIITIGQAIVYVMTGMYGDPAEMGAGICLLIIIQLFVAGL
                     IVLLLDELLQKGYGLGSGISLFIATNICETIVWKAFSPTTINTGRGTEFEGAVIALFH
                     LLATRTDKVRALREAFYRQNLPNLMNLIATVFVFAVVIYFQGFRVDLPIKSARYRGQY
                     SSYPIKLFYTSNIPIILQSALVSNLYVISQMLSVRFSGNFLVNLLGQWADVSGGGPAR
                     SYPVGGLCYYLSPPESMGAIFEDPVHVVVYIIFMLGSCAFFSKTWIEVSGSSAKDVAK
                     QLKEQQMVMRGHRDTSMVHELNRYIPTAAAFGGLCIGALSVLADFLGAIGSGTGILLA
                     VTIIYQYFEIFVKEQAEVGGMGALFF"
     exon            119..186
                     /gene="SEC61A2"
                     /inference="alignment:Splign:2.1.0"
     exon            187..252
                     /gene="SEC61A2"
                     /inference="alignment:Splign:2.1.0"
     exon            253..331
                     /gene="SEC61A2"
                     /inference="alignment:Splign:2.1.0"
     exon            332..463
                     /gene="SEC61A2"
                     /inference="alignment:Splign:2.1.0"
     exon            464..573
                     /gene="SEC61A2"
                     /inference="alignment:Splign:2.1.0"
     exon            574..727
                     /gene="SEC61A2"
                     /inference="alignment:Splign:2.1.0"
     exon            728..888
                     /gene="SEC61A2"
                     /inference="alignment:Splign:2.1.0"
     exon            889..1086
                     /gene="SEC61A2"
                     /inference="alignment:Splign:2.1.0"
     exon            1087..1278
                     /gene="SEC61A2"
                     /inference="alignment:Splign:2.1.0"
     exon            1279..1355
                     /gene="SEC61A2"
                     /inference="alignment:Splign:2.1.0"
     exon            1356..2495
                     /gene="SEC61A2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1894..1899
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SEC61A2"
                     /note="hexamer: AATAAA"
     polyA_site      1916
                     /gene="SEC61A2"
                     /note="major polyA site"
     regulatory      2430..2435
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SEC61A2"
                     /note="hexamer: AATAAA"
     polyA_site      2451
                     /gene="SEC61A2"
                     /note="major polyA site"
     regulatory      2490..2495
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SEC61A2"
                     /note="hexamer: ATTAAA"
     polyA_site      2495
                     /gene="SEC61A2"
ORIGIN      
        1 gcggggccgg taggatcgcg tcgggagccg gtaccgaggc ccgagccgcg ggagtcgagc
       61 gaaggcagcg ccgaggccgc ggtttccccc tgggcctccc cagcagcagc catgggcatc
      121 aaatttttag aagttatcaa accattctgt gcagttctac cagaaattca gaaaccggaa
      181 aggaaaatcc agtttagaga gaaggttctg tggactgcta taacgctctt cattttctta
      241 gtgtgttgtc agatcccact gtttggaatc atgtcatcag attctgcaga tcctttctac
      301 tggatgagag ttattctggc ttccaataga ggaactttaa tggaattggg tatctcccca
      361 attgtaacat ctggtttgat tatgcagttg ttagctggag ccaaaatcat tgaagttgga
      421 gatacaccga aagatagagc tttattcaat ggagcccaga aactgtttgg tatgatcatt
      481 accattgggc aagccattgt gtatgtcatg acggggatgt atggggaccc tgcagaaatg
      541 ggtgccggaa tctgtctcct gatcatcatt cagttgtttg ttgctggttt gattgtgctg
      601 ctgttagatg agctgctaca gaagggttac ggcttggggt ctgggatttc cctctttatt
      661 gccaccaaca tctgtgagac cattgtctgg aaggccttta gtcccactac cattaacact
      721 ggcagaggta ctgagtttga gggtgcagtc atagctctgt tccatttgtt ggccaccagg
      781 acggacaaag tccgagcttt acgggaggct ttttatcggc agaacttacc caatctcatg
      841 aacctcattg ctacagtttt tgtgtttgct gttgttatat atttccaagg atttcgcgtt
      901 gatctgccca ttaagtcggc ccgttaccga ggacagtaca gcagctaccc catcaaactc
      961 ttctacacct ccaacatccc catcatcctc cagtcggccc tggtgtccaa cctgtatgtt
     1021 atttcccaga tgctgtctgt tcgatttagt ggcaactttt tagtaaattt actaggacag
     1081 tgggccgatg tcagtggggg aggacccgca cgttcttacc cagttggagg cctttgttac
     1141 tatctttctc ctcctgagtc catgggcgcc atctttgagg atcctgtcca tgtcgttgtt
     1201 tatatcatct tcatgttggg gtcatgtgca ttcttctcta agacatggat tgaagtgtct
     1261 ggttcctcag ccaaagatgt agctaaacag ctgaaagaac agcagatggt aatgaggggc
     1321 caccgagata cctctatggt tcatgagctt aataggtaca tccccaccgc agctgcgttt
     1381 ggcggtttgt gcattggcgc cctgtcagtg ctggctgact tcctgggggc cattggatct
     1441 ggcactggaa ttctgctagc agtcactatt atttaccagt attttgaaat atttgttaaa
     1501 gaacaggccg aagttggtgg gatgggtgct ttgtttttct aaatgttcaa atatttcatt
     1561 ttgtgcgtgt gaaagggaaa acactttgac ggatcgtttt tgtcagatga cactggtggc
     1621 tccccttttc tcccctcaca gtttcttgtt tcgagtgctg actgacccgt ttctgaaatg
     1681 ggcaccgagc taagtctgtg tgcagcatta gtacccgctg ccttaaaact caagtttaca
     1741 ttattcatta aaaaaagtac atctagtgtt gcctgtaatg ctggaaacca gtgtatctac
     1801 cttgctgtgt taaatcatga cagtgagacg gtgagatgga ttcgttttgc acacaacatt
     1861 caaaacactt catattgccc ccacttgttg aaaaataaat gtagttcaaa ttgccacttt
     1921 ccagtatttt tgagcttatt taatgagttc tggaacattt atatctaatc tatattttag
     1981 ataattactt tttatacttt tttaactcat ggtatcccca ctccccaccc ccacctcatt
     2041 tttatttgtc ccttctcaaa gcagccactt agcccacatg ggcgaaatca agtctccagt
     2101 tatttctgcc acaactgctt ctaaggcctc ctccttctcc tcctcctctt cctcttcctc
     2161 cttttccttc tcctcctcct ctcttcccag tgacagcatc atcgtgctgt ttgcctgtat
     2221 tggctatgcc ttctaactcc aaccagtcac ttgagaatat tctttcaaga ttctgggccc
     2281 cgattctttt ctgtttaaat ccctaaagca aagatctaat tctcaagcaa tgtctgtagt
     2341 tcagtggggg tgaacaatga atatattcat gctaggaatt tgtgtctgtt gttgtactca
     2401 cagcagcaac atgagtgtaa acagtagaca ataaactttt atttaagaaa actgattcag
     2461 ttgtgttgga aaaaataaag aaatctgata ttaaa
//
