LOCUS       NM_014866               9018 bp    mRNA    linear   PRI 29-OCT-2024
DEFINITION  Homo sapiens SEC16 homolog A, endoplasmic reticulum export factor
            (SEC16A), transcript variant 1, mRNA.
ACCESSION   NM_014866 XM_005266135 XM_088459 XM_942490
VERSION     NM_014866.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9018)
  AUTHORS   Wang,M.J., Wang,Y.C., Masson,E., Wang,Y.H., Yu,D., Qian,Y.Y.,
            Tang,X.Y., Deng,S.J., Hu,L.H., Wang,L., Wang,L.J., Rebours,V.,
            Cooper,D.N., Ferec,C., Li,Z.S., Chen,J.M., Zou,W.B. and Liao,Z.
  TITLE     SEC16A Variants Predispose to Chronic Pancreatitis by Impairing
            ER-to-Golgi Transport and Inducing ER Stress
  JOURNAL   Adv Sci (Weinh) 11 (38), e2402550 (2024)
   PUBMED   39119875
  REMARK    GeneRIF: SEC16A Variants Predispose to Chronic Pancreatitis by
            Impairing ER-to-Golgi Transport and Inducing ER Stress.
REFERENCE   2  (bases 1 to 9018)
  AUTHORS   Lee,J.H., Han,J.H., Kim,H., Park,S.M., Joe,E.H. and Jou,I.
  TITLE     Parkinson's disease-associated LRRK2-G2019S mutant acts through
            regulation of SERCA activity to control ER stress in astrocytes
  JOURNAL   Acta Neuropathol Commun 7 (1), 68 (2019)
   PUBMED   31046837
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 9018)
  AUTHORS   Mayer,L., Jasztal,M., Pardo,M., Aguera de Haro,S., Collins,J.,
            Bariana,T.K., Smethurst,P.A., Grassi,L., Petersen,R., Nurden,P.,
            Favier,R., Yu,L., Meacham,S., Astle,W.J., Choudhary,J., Yue,W.W.,
            Ouwehand,W.H. and Guerrero,J.A.
  TITLE     Nbeal2 interacts with Dock7, Sec16a, and Vac14
  JOURNAL   Blood 131 (9), 1000-1011 (2018)
   PUBMED   29187380
  REMARK    GeneRIF: Nbeal2 interacts with Dock7, Sec16a, and Vac14.
REFERENCE   4  (bases 1 to 9018)
  AUTHORS   Wu,Y., Guo,X.P., Kanemoto,S., Maeoka,Y., Saito,A., Asada,R.,
            Matsuhisa,K., Ohtake,Y., Imaizumi,K. and Kaneko,M.
  TITLE     Sec16A, a key protein in COPII vesicle formation, regulates the
            stability and localization of the novel ubiquitin ligase RNF183
  JOURNAL   PLoS One 13 (1), e0190407 (2018)
   PUBMED   29300766
  REMARK    GeneRIF: Sec16A also stabilized the interacting ubiquitin ligase
            RNF152, which localizes to the lysosome and has structural
            similarity with RNF183
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 9018)
  AUTHORS   Maeda,M., Katada,T. and Saito,K.
  TITLE     TANGO1 recruits Sec16 to coordinately organize ER exit sites for
            efficient secretion
  JOURNAL   J Cell Biol 216 (6), 1731-1743 (2017)
   PUBMED   28442536
  REMARK    GeneRIF: Mammalian endoplasmic reticulum exit sites are organized
            by TANGO1 acting as a scaffold, in cooperation with Sec16 for
            efficient secretion.
REFERENCE   6  (bases 1 to 9018)
  AUTHORS   Hughes,H. and Stephens,D.J.
  TITLE     Sec16A defines the site for vesicle budding from the endoplasmic
            reticulum on exit from mitosis
  JOURNAL   J Cell Sci 123 (Pt 23), 4032-4038 (2010)
   PUBMED   21045114
  REMARK    GeneRIF: Sec16A remains associated with endoplasmic reticulum exit
            sites throughout mitosis.
REFERENCE   7  (bases 1 to 9018)
  AUTHORS   Hughes,H., Budnik,A., Schmidt,K., Palmer,K.J., Mantell,J.,
            Noakes,C., Johnson,A., Carter,D.A., Verkade,P., Watson,P. and
            Stephens,D.J.
  TITLE     Organisation of human ER-exit sites: requirements for the
            localisation of Sec16 to transitional ER
  JOURNAL   J Cell Sci 122 (Pt 16), 2924-2934 (2009)
   PUBMED   19638414
  REMARK    GeneRIF: These data are consistent with a model where Sec16 acts as
            a platform for COPII assembly at endoplasmic reticulum exit sites.
REFERENCE   8  (bases 1 to 9018)
  AUTHORS   Iinuma,T., Shiga,A., Nakamoto,K., O'Brien,M.B., Aridor,M.,
            Arimitsu,N., Tagaya,M. and Tani,K.
  TITLE     Mammalian Sec16/p250 plays a role in membrane traffic from the
            endoplasmic reticulum
  JOURNAL   J Biol Chem 282 (24), 17632-17639 (2007)
   PUBMED   17428803
  REMARK    GeneRIF: Results suggest that KIAA0310p, a mammalian homologue of
            yeast Sec16, builds up endoplasmic reticulum (ER) exit sites in
            cooperation with p125 and plays a role in membrane traffic from the
            ER.
REFERENCE   9  (bases 1 to 9018)
  AUTHORS   Bhattacharyya,D. and Glick,B.S.
  TITLE     Two mammalian Sec16 homologues have nonredundant functions in
            endoplasmic reticulum (ER) export and transitional ER organization
  JOURNAL   Mol Biol Cell 18 (3), 839-849 (2007)
   PUBMED   17192411
  REMARK    GeneRIF: Mammalian cells contain two distinct Sec16 homologues: a
            large protein Sec16L of 2154 aa and a smaller protein Sec16S of
            1060 aa.
REFERENCE   10 (bases 1 to 9018)
  AUTHORS   Watson,P., Townley,A.K., Koka,P., Palmer,K.J. and Stephens,D.J.
  TITLE     Sec16 defines endoplasmic reticulum exit sites and is required for
            secretory cargo export in mammalian cells
  JOURNAL   Traffic 7 (12), 1678-1687 (2006)
   PUBMED   17005010
  REMARK    GeneRIF: Sar1-GTP-dependent assembly of Sec16 on the endoplasmic
            reticulum(ER) membrane forms an organized scaffold defining ER exit
            sites
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AB002308.4 and BM975558.1.
            
            On Jun 2, 2019 this sequence version replaced NM_014866.1.
            
            Summary: This gene encodes a protein that forms part of the Sec16
            complex. This protein has a role in protein transport from the
            endoplasmic reticulum (ER) to the Golgi and mediates COPII vesicle
            formation at the transitional ER. Alternative splicing results in
            multiple transcript variants that encode different protein
            isoforms. [provided by RefSeq, Feb 2013].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB002308.4 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000684901.1/ ENSP00000508822.1
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-9016              AB002308.4         16-9031
            9017-9018           BM975558.1         27-28               c
FEATURES             Location/Qualifiers
     source          1..9018
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9q34.3"
     gene            1..9018
                     /gene="SEC16A"
                     /gene_synonym="KIAA0310; p250; SEC16L"
                     /note="SEC16 homolog A, endoplasmic reticulum export
                     factor"
                     /db_xref="GeneID:9919"
                     /db_xref="HGNC:HGNC:29006"
                     /db_xref="MIM:612854"
     exon            1..103
                     /gene="SEC16A"
                     /gene_synonym="KIAA0310; p250; SEC16L"
                     /inference="alignment:Splign:2.1.0"
     exon            104..225
                     /gene="SEC16A"
                     /gene_synonym="KIAA0310; p250; SEC16L"
                     /inference="alignment:Splign:2.1.0"
     exon            226..3861
                     /gene="SEC16A"
                     /gene_synonym="KIAA0310; p250; SEC16L"
                     /inference="alignment:Splign:2.1.0"
     CDS             295..7368
                     /gene="SEC16A"
                     /gene_synonym="KIAA0310; p250; SEC16L"
                     /EC_number="3.1.3.36"
                     /note="isoform 1 is encoded by transcript variant 1;
                     protein transport protein Sec16A; protein SEC16 homolog A"
                     /codon_start=1
                     /product="protein transport protein Sec16A isoform 1"
                     /protein_id="NP_055681.1"
                     /db_xref="CCDS:CCDS55351.1"
                     /db_xref="GeneID:9919"
                     /db_xref="HGNC:HGNC:29006"
                     /db_xref="MIM:612854"
                     /translation="MQPPPQTVPSGMAGPPPAGNPRSVFWASSPYRRRANNNAAVAPT
                     TCPLQPVTDPFAFSRQALQSTPLGSSSKSSPPVLQGPAPAGFSQHPGLLVPHTHARDS
                     SQGPCEPLPGPLTQPRAHASPFSGALTPSAPPGPEMNRSAEVGPSSEPEVQTLPYLPH
                     YIPGVDPETSHGGHPHGNMPGLDRPLSRQNPHDGVVTPAASPSLPQPGLQMPGQWGPV
                     QGGPQPSGQHRSPCPEGPVPSGVPCATSVPHFPTPSILHQGPGHEQHSPLVAPPAALP
                     SDGRDEVSHLQSGSHLANNSDPESTFRQNPRIVNHWASPELRQNPGVKNEHRPASALV
                     NPLARGDSPENRTHHPLGAGAGSGCAPLEADSGASGALAMFFQGGETENEENLSSEKA
                     GLSGQADFDDFCSSPGLGRPPAPTHVGAGSLCQALLPGPSNEAAGDVWGDTASTGVPD
                     ASGSQYENVENLEFVQNQEVLPSEPLNLDPSSPSDQFRYGPLPGPAVPRHGAVCHTGA
                     PDATLHTVHPDSVSSSYSSRSHGRLSGSARPQELVGTFIQQEVGKPEDEASGSFFKQI
                     DSSPVGGETDETTVSQNYRGSVSQPSTPSPPKPTGIFQTSANSSFEPVKSHLVGVKPF
                     EADRANVVGEVRETCVRQKQCRPAAALPDASPGNLEQPPDNMETLCAPQVCPLPLNST
                     TEAVHMLPHAGAPPLDTVYPAPEKRPSARTQGPVKCESPATTLWAQSELPDFGGNVLL
                     APAAPALYVCAKPQPPVVQPPEEAMSGQQSRNPSSAAPVQSRGGIGASENLENPPKMG
                     EEEALQSQASSGYASLLSSPPTESLQNPPVLIAQPDHSYNLAQPINFSVSLSNSHEKN
                     QSWREALVGDRPAVSSWALGGDSGENTSLSGIPTSSVLSLSLPSSVAQSNFPQGSGAS
                     EMVSNQPANLLVQPPSQPVPENLVPESQKDRKAGSALPGFANSPAGSTSVVLVPPAHG
                     TLVPDGNKANHSSHQEDTYGALDFTLSRTLENPVNVYNPSHSDSLASQQSVASHPRQS
                     GPGAPNLDRFYQQVTKDAQGQPGLERAQQELVPPQQQASPPQLPKAMFSELSNPESLP
                     AQGQAQNSAQSPASLVLVDAGQQLPPRPPQSSSVSLVSSGSGQAAVPSEQPWPQPVPA
                     LAPGPPPQDLAAYYYYRPLYDAYQPQYSLPYPPEPGAASLYYQDVYSLYEPRYRPYDG
                     AASAYAQNYRYPEPERPSSRASHSSERPPPRQGYPEGYYSSKSGWSSQSDYYASYYSS
                     QYDYGDPGHWDRYHYSARVRDPRTYDRRYWCDAEYDAYRREHSAFGDRPEKRDNNWRY
                     DPRFTGSFDDDPDPHRDPYGEEVDRRSVHSEHSARSLHSAHSLASRRSSLSSHSHQSQ
                     IYRSHNVAAGSYEAPLPPGSFHGDFAYGTYRSNFSSGPGFPEYGYPADTVWPAMEQVS
                     SRPTSPEKFSVPHVCARFGPGGQLIKVIPNLPSEGQPALVEVHSMEALLQHTSEQEEM
                     RAFPGPLAKDDTHKVDVINFAQNKAMKCLQNENLIDKESASLLWNFIVLLCRQNGTVV
                     GTDIAELLLRDHRTVWLPGKSPNEANLIDFTNEAVEQVEEEESGEAQLSFLTGGPAAA
                     ASSLERETERFRELLLYGRKKDALESAMKNGLWGHALLLASKMDSRTHARVMTRFANS
                     LPINDPLQTVYQLMSGRMPAASTCCGDEKWGDWRPHLAMVLSNLNNNMDVESRTMATM
                     GDTLASRGLLDAAHFCYLMAQAGFGVYTKKTTKLVLIGSNHSLPFLKFATNEAIQRTE
                     AYEYAQSLGAETCPLPSFQVFKFIYSCRLAEMGLATQAFHYCEAIAKSILTQPHLYSP
                     VLISQLVQMASQLRLFDPQLKEKPEEESLAAPTWLVHLQQVERQIKEGAGVWHQDGAL
                     PQQCPGTPSSEMEQLDRPGLSQPGALGIANPLLAVPAPSPEHSSPSVRLLPSAPQTLP
                     DGPLASPARVPMFPVPLPPGPLEPGPGCVTPGPALGFLEPSGPGLPPGVPPLQERRHL
                     LQEARSPDPGIVPQEAPVGNSLSELSEENFDGKFANLTPSRTVPDSEAPPGWDRADSG
                     PTQPPLSLSPAPETKRPGQAAKKETKEPKKGESWFFRWLPGKKKTEAYLPDDKNKSIV
                     WDEKKNQWVNLNEPEEEKKAPPPPPTSMPKTVQAAPPALPGPPGAPVNMYSRRAAGTR
                     ARYVDVLNPSGTQRSEPALAPADFVAPLAPLPIPSNLFVPTPDAEEPQLPDGTGREGP
                     AAARGLANPEPAPEPKVLSSAASLPGSELPSSRPEGSQGGELSRCSSMSSLSREVSQH
                     FNQAPGDLPAAGGPPSGAMPFYNPAQLAQACATSGSSRLGRIGQRKHLVLN"
     exon            3862..3998
                     /gene="SEC16A"
                     /gene_synonym="KIAA0310; p250; SEC16L"
                     /inference="alignment:Splign:2.1.0"
     exon            3999..4096
                     /gene="SEC16A"
                     /gene_synonym="KIAA0310; p250; SEC16L"
                     /inference="alignment:Splign:2.1.0"
     exon            4097..4223
                     /gene="SEC16A"
                     /gene_synonym="KIAA0310; p250; SEC16L"
                     /inference="alignment:Splign:2.1.0"
     exon            4224..4422
                     /gene="SEC16A"
                     /gene_synonym="KIAA0310; p250; SEC16L"
                     /inference="alignment:Splign:2.1.0"
     exon            4423..4597
                     /gene="SEC16A"
                     /gene_synonym="KIAA0310; p250; SEC16L"
                     /inference="alignment:Splign:2.1.0"
     exon            4598..4740
                     /gene="SEC16A"
                     /gene_synonym="KIAA0310; p250; SEC16L"
                     /inference="alignment:Splign:2.1.0"
     exon            4741..4802
                     /gene="SEC16A"
                     /gene_synonym="KIAA0310; p250; SEC16L"
                     /inference="alignment:Splign:2.1.0"
     exon            4803..4941
                     /gene="SEC16A"
                     /gene_synonym="KIAA0310; p250; SEC16L"
                     /inference="alignment:Splign:2.1.0"
     exon            4942..5187
                     /gene="SEC16A"
                     /gene_synonym="KIAA0310; p250; SEC16L"
                     /inference="alignment:Splign:2.1.0"
     exon            5188..5285
                     /gene="SEC16A"
                     /gene_synonym="KIAA0310; p250; SEC16L"
                     /inference="alignment:Splign:2.1.0"
     exon            5286..5367
                     /gene="SEC16A"
                     /gene_synonym="KIAA0310; p250; SEC16L"
                     /inference="alignment:Splign:2.1.0"
     exon            5368..5485
                     /gene="SEC16A"
                     /gene_synonym="KIAA0310; p250; SEC16L"
                     /inference="alignment:Splign:2.1.0"
     exon            5486..5597
                     /gene="SEC16A"
                     /gene_synonym="KIAA0310; p250; SEC16L"
                     /inference="alignment:Splign:2.1.0"
     exon            5598..5703
                     /gene="SEC16A"
                     /gene_synonym="KIAA0310; p250; SEC16L"
                     /inference="alignment:Splign:2.1.0"
     exon            5704..5844
                     /gene="SEC16A"
                     /gene_synonym="KIAA0310; p250; SEC16L"
                     /inference="alignment:Splign:2.1.0"
     exon            5845..5958
                     /gene="SEC16A"
                     /gene_synonym="KIAA0310; p250; SEC16L"
                     /inference="alignment:Splign:2.1.0"
     exon            5959..6151
                     /gene="SEC16A"
                     /gene_synonym="KIAA0310; p250; SEC16L"
                     /inference="alignment:Splign:2.1.0"
     exon            6152..6370
                     /gene="SEC16A"
                     /gene_synonym="KIAA0310; p250; SEC16L"
                     /inference="alignment:Splign:2.1.0"
     exon            6371..6453
                     /gene="SEC16A"
                     /gene_synonym="KIAA0310; p250; SEC16L"
                     /inference="alignment:Splign:2.1.0"
     exon            6454..6606
                     /gene="SEC16A"
                     /gene_synonym="KIAA0310; p250; SEC16L"
                     /inference="alignment:Splign:2.1.0"
     exon            6607..6684
                     /gene="SEC16A"
                     /gene_synonym="KIAA0310; p250; SEC16L"
                     /inference="alignment:Splign:2.1.0"
     exon            6685..6741
                     /gene="SEC16A"
                     /gene_synonym="KIAA0310; p250; SEC16L"
                     /inference="alignment:Splign:2.1.0"
     exon            6742..6853
                     /gene="SEC16A"
                     /gene_synonym="KIAA0310; p250; SEC16L"
                     /inference="alignment:Splign:2.1.0"
     exon            6854..6991
                     /gene="SEC16A"
                     /gene_synonym="KIAA0310; p250; SEC16L"
                     /inference="alignment:Splign:2.1.0"
     exon            6992..7086
                     /gene="SEC16A"
                     /gene_synonym="KIAA0310; p250; SEC16L"
                     /inference="alignment:Splign:2.1.0"
     exon            7087..7161
                     /gene="SEC16A"
                     /gene_synonym="KIAA0310; p250; SEC16L"
                     /inference="alignment:Splign:2.1.0"
     exon            7162..7221
                     /gene="SEC16A"
                     /gene_synonym="KIAA0310; p250; SEC16L"
                     /inference="alignment:Splign:2.1.0"
     exon            7222..7299
                     /gene="SEC16A"
                     /gene_synonym="KIAA0310; p250; SEC16L"
                     /inference="alignment:Splign:2.1.0"
     exon            7300..9018
                     /gene="SEC16A"
                     /gene_synonym="KIAA0310; p250; SEC16L"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8994..8999
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SEC16A"
                     /gene_synonym="KIAA0310; p250; SEC16L"
                     /note="hexamer: AATAAA"
     polyA_site      9018
                     /gene="SEC16A"
                     /gene_synonym="KIAA0310; p250; SEC16L"
                     /note="major polyA site"
ORIGIN      
        1 ggatgtgcca agatggctgc ggcggctgag gtgtctgtgc tcgtcgccag cgtcgggtgg
       61 gctttcgccc gcggctcctg agggatcggt ctcagccgcg cggagaccgt cttgctctgt
      121 tgcccaggct ggaccgcggt ggtataatca tagctcactg cagcctcgaa ctcatggaca
      181 caagtgatct tccttcctta accccctgag tccctgggac tacagctcca attaaggaac
      241 agctatatcc tgcttccttc tgtaagcagc atcgacttgt gcaagggttc agtcatgcag
      301 ccaccgcccc agacggtccc gtctggcatg gctgggccac ctccagccgg gaatcctcgg
      361 agcgtgttct gggctagcag cccttacagg agacgggcta ataataatgc agcagtggct
      421 ccgacaactt gcccgttgca gccggtcacg gatccatttg cttttagtag acaggcgctc
      481 caaagtacac cactgggcag ttcgtccaaa agcagtccac ctgtcttgca aggcccagcc
      541 cccgcagggt tttctcagca ccccggtttg cttgttcctc acacacatgc cagagatagc
      601 tctcagggac cctgtgagcc cctgcctgga cctctgacac agcccagagc acatgccagt
      661 ccgttttctg gtgcattgac accttcagca cctcctgggc ctgagatgaa caggagtgca
      721 gaggtcggtc ccagttcaga gcctgaagtt cagactctgc catatcttcc tcactacatt
      781 ccaggagtgg atcctgaaac gtctcatggg ggccaccctc atgggaacat gcctgggctc
      841 gaccgacccc tgagcaggca aaacccacat gacggtgtgg tcaccccagc agcatcccct
      901 tccctccctc agcctggtct gcagatgcca ggacagtggg ggccagtgca gggaggccca
      961 cagccctcgg ggcaacatcg ttcaccctgc cctgaaggac ctgttcccag cggggtgccc
     1021 tgtgccacca gcgttcctca tttccccacc ccgtccatcc tacatcaggg ccctggtcat
     1081 gagcaacaca gccctctggt ggctccccca gcagccttgc ccagtgacgg aagagacgag
     1141 gtgagccact tgcaaagtgg aagccacctg gccaataact ctgatcctga aagtacattc
     1201 aggcaaaatc ccagaattgt gaatcactgg gcaagcccag agctcaggca gaatccagga
     1261 gtgaagaatg agcaccggcc cgcctctgct cttgtgaacc ccctcgcccg gggagatagc
     1321 ccagaaaacc gtacgcacca cccactgggg gctggggccg ggtctggctg tgccccgcta
     1381 gaagcagact caggagcttc aggagctctg gcgatgtttt tccaaggggg agagacagaa
     1441 aatgaggaga atctctcatc tgaaaaagca ggcttatctg gtcaagcgga ctttgacgat
     1501 ttctgctcca gccctgggct aggccgtccg cccgcaccta cacacgtggg ggcaggcagc
     1561 ctctgccagg cccttctccc aggccccagc aatgaggctg ctggtgatgt gtggggtgac
     1621 acagcgagca caggggtgcc ggatgccagc ggctcgcagt atgagaatgt tgagaactta
     1681 gaatttgttc agaatcaaga agttctgcca agtgagcccc tcaatttgga cccttcctcc
     1741 ccgagtgacc agttcagata tgggcccctt cctgggccag ctgtgcccag gcatggtgct
     1801 gtgtgccaca ccggagcccc tgatgccaca ctgcatacag tgcaccctga cagcgtgtca
     1861 tccagctata gcagcagaag ccacggaagg ctctcaggct cagccaggcc ccaggagctg
     1921 gttggcacat tcattcagca agaagttgga aaacccgagg atgaagcttc aggtagtttt
     1981 tttaagcaaa tcgattcttc tcccgtagga ggtgaaacag acgagaccac tgtgagccag
     2041 aattaccgtg gcagcgtgtc ccagccctca accccgagcc ccccgaaacc tacaggaata
     2101 tttcagacaa gtgcaaatag ttctttcgaa ccggtaaaat ctcacttagt tggggtaaaa
     2161 ccatttgagg cagatcgcgc caacgtggtt ggtgaagtaa gggagacctg tgtccgccag
     2221 aagcagtgca gaccagctgc cgccctgccc gatgcttccc ctggcaacct ggagcagcca
     2281 ccagacaaca tggagaccct ctgtgcaccc caggtctgtc ccctgcctct taactccacc
     2341 acggaagctg tgcacatgct tccgcacgca ggggcaccgc ccttggatac tgtgtatcca
     2401 gcacccgaga agaggccttc agccaggacc caggggcccg tgaagtgtga gagcccagca
     2461 acgactctgt gggcgcaaag tgagctgcca gattttggag gcaacgtcct tctggcccct
     2521 gcagccccgg cgctttatgt gtgtgcaaaa cctcagccac ctgttgttca gcctccagaa
     2581 gaggcgatgt ccgggcagca gtcacggaac ccaagctcgg cggccccggt gcagagccga
     2641 ggtggcattg gtgcttctga gaaccttgag aatcctccca aaatgggaga ggaggaggcc
     2701 cttcagtccc aggcgagttc tggttatgca agtttattat cctcaccgcc cactgagtct
     2761 ctgcagaatc ctccagtctt gattgctcag cctgatcaca gctataatct ggctcagccc
     2821 attaactttt ctgtgtcctt atcgaactct catgagaaga atcagtcctg gagagaggct
     2881 ttggtggggg atagacctgc agtcagcagt tgggctctcg gtggtgattc tggggagaac
     2941 acttctttgt ctgggattcc aaccagctct gtccttagct tgtctctgcc tagcagtgtt
     3001 gcccaaagta attttccaca aggttctggt gcttccgaaa tggtttctaa tcagcctgct
     3061 aatttgctgg ttcaaccacc atcccagcca gttccagaga acttggttcc agaaagtcaa
     3121 aaggatcgta aggcaggaag tgctcttccc ggatttgcta atagccctgc tggaagcaca
     3181 agtgtggtgt tagttccacc tgcacacggc accctggtgc ctgatggtaa taaggcaaac
     3241 cattccagtc atcaggaaga cacttacgga gccctagact ttaccttaag caggactttg
     3301 gaaaatcctg taaacgtgta caacccgtcc cattctgaca gcctcgcttc tcagcaaagt
     3361 gttgccagtc atcccagaca atctgggcct ggggcgccta accttgaccg tttttatcag
     3421 caggtcacga aagatgccca gggccagcct ggcctcgaaa gagcccagca ggagctggtg
     3481 ccaccccagc aacaggcttc tcccccacaa ctacccaaag ccatgttttc ggagctgtca
     3541 aatccagaaa gtctgcccgc acagggacag gcccagaact cagcacagtc accagcaagt
     3601 ctggttctgg tcgacgcggg tcagcagctg ccccctcggc ctcctcagtc ctctagcgtg
     3661 tctctggtgt ccagtggctc cggccaggca gctgtgccgt cagagcagcc gtggccacag
     3721 ccagtgcctg cacttgcccc cggcccaccg cctcaggacc tggccgccta ctactactac
     3781 cggcctttgt acgatgccta ccagcctcag tactctttgc cgtacccacc ggagcctggc
     3841 gcagcctccc tctattacca ggatgtctac agcctctatg agcctcgata caggccctat
     3901 gatggtgctg cgtctgctta cgcccagaac taccgctatc ccgagcccga gcggcccagc
     3961 tcccgagcca gccactcctc ggaacggcca cctcccaggc aaggatatcc tgaaggatac
     4021 tatagttcca aaagtggatg gagcagtcag agcgattact atgcaagcta ttactccagc
     4081 cagtacgatt atggagatcc aggtcactgg gatcgttacc actacagtgc tagagtcagg
     4141 gacccccgca cctatgaccg gaggtattgg tgtgatgcag agtatgacgc atacaggaga
     4201 gagcactctg ccttcgggga caggcccgag aaacgtgaca acaactggag gtacgatcct
     4261 cgcttcacgg ggagttttga cgatgacccc gatccgcaca gagaccctta tggggaagag
     4321 gtggaccggc gcagcgtcca cagcgagcac tcggcacgga gcctgcacag cgcacacagc
     4381 ctggccagcc gccgcagcag cctcagctcc cactcgcacc agagtcagat ttacagaagc
     4441 cacaatgtgg ctgccggttc ctacgaggcc ccgcttcctc caggctcctt tcacggcgat
     4501 tttgcctacg gcacctaccg cagcaatttc agcagtggcc ccggcttccc agagtatggc
     4561 taccctgccg acaccgtctg gcctgccatg gagcaagttt catcaagacc aacttctcct
     4621 gaaaaatttt cagtgcctca tgtctgtgcc aggtttggcc ctggcggtca gcttatcaaa
     4681 gtgattccca atctgccttc agaaggacag ccggccttgg tggaggtcca cagcatggag
     4741 gccttgctgc agcacacgtc tgagcaggag gagatgcggg cgttcccggg acccctggcc
     4801 aaagacgaca cccataaggt ggatgtcatt aattttgcac agaacaaagc tatgaaatgt
     4861 ttgcagaatg aaaacttaat tgacaaagag tctgcaagtc ttctttggaa ttttattgtt
     4921 ctcttatgca gacaaaatgg gaccgtggta gggaccgaca ttgcggagct tctgttacga
     4981 gaccacagaa cagtgtggct tcctgggaag tcgcccaatg aagcaaacct gattgatttc
     5041 acgaatgagg cagtggagca ggtggaagag gaggagtctg gtgaggccca gctctctttc
     5101 ctcactggtg gtccggcggc tgccgccagc tcgctcgaga gagagaccga gaggttcagg
     5161 gagctgttgc tgtatggccg taagaaggat gctttggagt ctgcaatgaa gaatggcctg
     5221 tggggtcacg ctctgctact tgcaagtaag atggacagcc ggacacacgc ccgagtcatg
     5281 accaggtttg ctaacagcct cccaatcaac gaccctctgc agacagtcta ccagctcatg
     5341 tccggacgga tgcctgccgc gtccacgtgc tgtggagacg agaaatgggg agattggagg
     5401 ccgcacctcg ccatggtctt gtccaacttg aacaacaaca tggacgtcga gtccaggacg
     5461 atggctacca tgggcgacac tctggcttca aggggcctct tggatgcggc ccacttctgc
     5521 tacctcatgg cccaggcggg atttggtgtt tacacgaaga aaactacaaa gcttgtctta
     5581 atcggatcca atcacagttt gccattctta aagttcgcaa ccaacgaagc aatccagagg
     5641 acggaagcct atgagtacgc ccagtccctg ggtgccgaga cctgccccct gcctagtttc
     5701 caggtgttta agttcatcta ctcctgccgc ctggcggaaa tggggctggc cacgcaagcc
     5761 ttccactact gtgaggccat cgcgaagagc atcctgacgc agccgcacct gtattccccg
     5821 gtgttgatca gccagcttgt gcagatggct tcccagttac gactcttcga tccccagctg
     5881 aaagagaagc cagaagagga gtccttggcc gcacccacgt ggctggttca cctgcagcag
     5941 gtggagcggc agattaagga gggggctgga gtatggcatc aggatggagc cctcccgcag
     6001 cagtgtcctg gcactccgag ttccgagatg gagcagttgg acaggccagg actcagtcag
     6061 ccaggagccc tggggatcgc caaccctctg ctggcggtgc ctgcaccgag ccctgagcac
     6121 tcgagcccga gcgtgcggct gctgccctca gctccgcaga cgctccctga cggcccattg
     6181 gccagtcctg ccagagtgcc gatgttccca gtgccactgc ccccggggcc cctggagccg
     6241 ggtcctggct gtgtgacccc agggcctgca cttggcttcc tggagccctc cgggcctggc
     6301 ctcccacctg gtgtgccacc tctgcaggaa aggagacact tgctccagga agccaggagc
     6361 ccagacccag ggatagtgcc gcaggaggcg cctgttggaa actcactttc cgagctaagc
     6421 gaagaaaatt ttgatggaaa atttgctaat ctgaccccct cgaggacggt gccagactcg
     6481 gaggcccccc cagggtggga tcgtgccgac tcgggtccca cgcagccacc tctgtctctc
     6541 tcacccgctc ccgaaacaaa gagacccgga caggcagcca agaaagaaac gaaggaacct
     6601 aagaagggtg aatcctggtt ctttcgttgg ctacctggaa agaaaaagac agaagcttat
     6661 ttgccagatg acaagaacaa atcgattgtt tgggatgaaa agaaaaacca gtgggtgaat
     6721 ttaaatgagc cagaagagga gaagaaagcc ccgcccccac ctccaacctc gatgcccaag
     6781 actgtgcaag ctgccccgcc tgccctccca gggcctcctg gagcccccgt gaacatgtac
     6841 tctagaagag cagcaggaac cagagctcgc tacgttgacg tcctgaaccc aagcgggacc
     6901 cagcggagcg agccggctct cgctcctgcg gactttgtcg ctccactcgc gccactccca
     6961 attccttcta acttgttcgt gccaacccca gatgcagaag aaccacagct tccagacggg
     7021 actggcaggg aagggcctgc agcagctagg ggcctggcca atccagagcc tgccccagag
     7081 cccaaggttt taagctctgc agcgtcactc cctggctctg aactcccctc ctccaggcct
     7141 gagggttccc agggaggaga gctttcgcgc tgtagttcaa tgagttcatt atcacgtgaa
     7201 gtgagccagc attttaatca ggctcctggc gacctccctg ctgcaggggg ccctcccagc
     7261 ggggccatgc ccttctacaa ccctgctcag ctggcacagg cctgcgccac ctccgggagc
     7321 tcaaggctag ggaggattgg ccagaggaag cacctggtgc tgaactaggc ttgccctgct
     7381 gtgaacttgc acttggagcc ctgacgctgc tgttctcccc gaagaacccg accgacctcc
     7441 gcgatctccg tcccgccccc agggagacac agcagtgact cagagctggt cgcacactgt
     7501 gcctccctcc tcaccgccca tcgtaatgaa ttattttgaa aattaattcc accatccttt
     7561 cagattctgg atggaaagac tgaatctttg actcagaatt gtttgccgaa aagaatgatg
     7621 tgactttctt agtcatttag gatgatttaa ggatatagta ttcctggtca tttaagaatg
     7681 ttcattcatt gaagccggag ctgtctctgc cacaggagag ccacatggtc ggtagtaacc
     7741 agggcctctc caagcccagc tgtgagtcac tgcccagtga gtcccgcgct tcctttaagg
     7801 tgctgggagc aaagagaggg tgactgaggc agaccccaac ccctgctctg caccatctgg
     7861 gccctcgccg tgtttgaacc tggctgaatg agtggagggc gctgtgttct caatcagcgc
     7921 ctccgaggag ccgtggggtt ccttcggcat tagttcacgg tttttgagag aggccttagt
     7981 tactgcagtg aatttctttc ctgttgcaaa gacgcttcca gcctcacttt actttctgtg
     8041 gcctgatgag gaccatgggt gattttgtgt acccaaagcg ctggggactg cccaccgtgt
     8101 ggcccagtca ctgggaagga gccccagaga gccggctgtc tgacatgatg gctcagggtg
     8161 gtcatccagg ttgaaaactg accgtgtgat gtttgatttg ggcttcattt cgtgtgtagg
     8221 agcacggtta gactcactgt taaggaagct ggatgcactt ctctaaaagg ctgcactttc
     8281 cgtgagcact tttcgtggta caatccacat gacccacttt ctcccctggg ggacgttggt
     8341 tcagaggttg gtagcacttg gggagagtat cttaacacag tttcttgaca gcagctctgg
     8401 aacttagtat ttctgccccg agttttgcca cactgagact ttgagtagct cctggtggac
     8461 tcaaccctgt tcaactcaga gacgggcctc ctctcactga tgcaaagctt taaggcttct
     8521 ctgactgttc tgaaactctt cgtattcttg tcaagtctaa agagactgaa gaaaagattt
     8581 aaatactaat aaaaatcagt agataatttc tgtaggttct gctggaggaa tacaaactgt
     8641 ttggtgtttt aaatttaagt gtagaaattg tagaatgtgg aattagcaca gatccttcct
     8701 ggctttctgt ttcacttgat catttagccc agaccaccca ggatgttttc caaaatgttc
     8761 cacaggcgtg tcccgctgga tccatttgtc cttgtcactt ggagaaaggc cagtccctgt
     8821 gacggggcag ccctctctgt ccctcggtca gctcgtgtga atcctgggac ctcttccggt
     8881 cggctctgcc cgctgttctg gggtcgactg ccacgacttt tgattcaaga agcttcctcc
     8941 aggcgggagc ggctattttt cctaaatgag aattgttaca ttgcaaattg ttgaataaaa
     9001 tattttgcgc tccttcaa
//
