LOCUS       NM_017988               5977 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens SCY1 like pseudokinase 2 (SCYL2), transcript variant
            1, mRNA.
ACCESSION   NM_017988
VERSION     NM_017988.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5977)
  AUTHORS   Seidahmed,M.Z., Al-Kindi,A., Alsaif,H.S., Miqdad,A., Alabbad,N.,
            Alfifi,A., Abdelbasit,O.B., Alhussein,K., Alsamadi,A., Ibrahim,N.,
            Al-Futaisi,A., Al-Maawali,A. and Alkuraya,F.S.
  TITLE     Recessive mutations in SCYL2 cause a novel syndromic form of
            arthrogryposis in humans
  JOURNAL   Hum Genet 139 (4), 513-519 (2020)
   PUBMED   31960134
  REMARK    GeneRIF: results suggest that severe neurogenic arthrogryposis with
            brain malformation is the human phenotypic consequence of SCYL2
            loss of function mutations.
REFERENCE   2  (bases 1 to 5977)
  AUTHORS   Miyakawa,K., Sawasaki,T., Matsunaga,S., Tokarev,A., Quinn,G.,
            Kimura,H., Nomaguchi,M., Adachi,A., Yamamoto,N., Guatelli,J. and
            Ryo,A.
  TITLE     Interferon-induced SCYL2 limits release of HIV-1 by triggering
            PP2A-mediated dephosphorylation of the viral protein Vpu
  JOURNAL   Sci Signal 5 (245), ra73 (2012)
   PUBMED   23047923
  REMARK    GeneRIF: results suggest that SCYL2 serves as a regulatory factor
            for Vpu, reducing the extent of Vpu phosphorylation and
            consequently enhancing BST2-mediated viral restriction.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5977)
  AUTHORS   Terabayashi,T., Funato,Y., Fukuda,M. and Miki,H.
  TITLE     A coated vesicle-associated kinase of 104 kDa (CVAK104) induces
            lysosomal degradation of frizzled 5 (Fzd5)
  JOURNAL   J Biol Chem 284 (39), 26716-26724 (2009)
   PUBMED   19643732
  REMARK    GeneRIF: Studies identify CVAK104 as a novel binding partner of
            Dishevelled (Dvl) and that CVAK104 also interacts with Fzd5.
REFERENCE   4  (bases 1 to 5977)
  AUTHORS   Borner,G.H., Rana,A.A., Forster,R., Harbour,M., Smith,J.C. and
            Robinson,M.S.
  TITLE     CVAK104 is a novel regulator of clathrin-mediated SNARE sorting
  JOURNAL   Traffic 8 (7), 893-903 (2007)
   PUBMED   17587408
  REMARK    GeneRIF: CVAK104 is an evolutionarily conserved protein involved in
            SNARE sorting that is essential for normal embryonic development
REFERENCE   5  (bases 1 to 5977)
  AUTHORS   Duwel,M. and Ungewickell,E.J.
  TITLE     Clathrin-dependent association of CVAK104 with endosomes and the
            trans-Golgi network
  JOURNAL   Mol Biol Cell 17 (10), 4513-4525 (2006)
   PUBMED   16914521
  REMARK    GeneRIF: Together, our data suggest a function for CVAK104 in
            clathrin-dependent pathways between the trans-Golgi network and the
            endosomal system.
REFERENCE   6  (bases 1 to 5977)
  AUTHORS   Schmid,E.M., Ford,M.G., Burtey,A., Praefcke,G.J., Peak-Chew,S.Y.,
            Mills,I.G., Benmerah,A. and McMahon,H.T.
  TITLE     Role of the AP2 beta-appendage hub in recruiting partners for
            clathrin-coated vesicle assembly
  JOURNAL   PLoS Biol 4 (9), e262 (2006)
   PUBMED   16903783
REFERENCE   7  (bases 1 to 5977)
  AUTHORS   Conner,S.D. and Schmid,S.L.
  TITLE     CVAK104 is a novel poly-L-lysine-stimulated kinase that targets the
            beta2-subunit of AP2
  JOURNAL   J Biol Chem 280 (22), 21539-21544 (2005)
   PUBMED   15809293
  REMARK    GeneRIF: CVAK104 binds ATP and functions in vitro as a
            poly-L-lysine-stimulated kinase that is capable of
            autophosphorylation and phosphorylating the beta2-adaptin subunit
            of AP2.
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC026110.42, DB127407.1,
            BC063798.1, AB037781.1 and CN337316.1.
            
            On May 17, 2019 this sequence version replaced NM_017988.5.
            
            Summary: The protein encoded by this gene associates with
            clathrin-coated complexes at the plasma membrane and with endocytic
            coated vesicles. The encoded protein phosphorylates the beta2
            subunit of the plasma membrane adapter complex AP2 and interacts
            with clathrin, showing involvement in clathrin-dependent pathways
            between the trans-Golgi network and the endosomal system. In
            addition, this protein has a role in the Wnt signaling pathway by
            targeting frizzled 5 (Fzd5) for lysosomal degradation. Two
            transcript variants encoding the same protein have been found for
            this gene. [provided by RefSeq, Dec 2015].
            
            Transcript Variant: This variant (1) encodes isoform 1. Both
            variants 1 and 2 encode the same protein.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660807.121856.1,
                                           SRR1660809.195646.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000360820.7/ ENSP00000354061.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-207               AC026110.42        102354-102560       c
            208-594             DB127407.1         1-387
            595-4401            BC063798.1         1-3807
            4402-5712           AB037781.1         3361-4671
            5713-5977           CN337316.1         1-265
FEATURES             Location/Qualifiers
     source          1..5977
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q23.1"
     gene            1..5977
                     /gene="SCYL2"
                     /gene_synonym="AMC4; AMCNACC; CVAK104"
                     /note="SCY1 like pseudokinase 2"
                     /db_xref="GeneID:55681"
                     /db_xref="HGNC:HGNC:19286"
                     /db_xref="MIM:616365"
     exon            1..616
                     /gene="SCYL2"
                     /gene_synonym="AMC4; AMCNACC; CVAK104"
                     /inference="alignment:Splign:2.1.0"
     exon            617..821
                     /gene="SCYL2"
                     /gene_synonym="AMC4; AMCNACC; CVAK104"
                     /inference="alignment:Splign:2.1.0"
     CDS             645..3434
                     /gene="SCYL2"
                     /gene_synonym="AMC4; AMCNACC; CVAK104"
                     /note="isoform 1 is encoded by transcript variant 1;
                     coated vesicle-associated kinase of 104 kDa; SCY1-like
                     protein 2; SCY1-like 2; SCY1-like, kinase-like 2"
                     /codon_start=1
                     /product="SCY1-like protein 2 isoform 1"
                     /protein_id="NP_060458.3"
                     /db_xref="CCDS:CCDS9076.1"
                     /db_xref="GeneID:55681"
                     /db_xref="HGNC:HGNC:19286"
                     /db_xref="MIM:616365"
                     /translation="MESMLNKLKSTVTKVTADVTSAVMGNPVTREFDVGRHIASGGNG
                     LAWKIFNGTKKSTKQEVAVFVFDKKLIDKYQKFEKDQIIDSLKRGVQQLTRLRHPRLL
                     TVQHPLEESRDCLAFCTEPVFASLANVLGNWENLPSPISPDIKDYKLYDVETKYGLLQ
                     VSEGLSFLHSSVKMVHGNITPENIILNKSGAWKIMGFDFCVSSTNPSEQEPKFPCKEW
                     DPNLPSLCLPNPEYLAPEYILSVSCETASDMYSLGTVMYAVFNKGKPIFEVNKQDIYK
                     SFSRQLDQLSRLGSSSLTNIPEEVREHVKLLLNVTPTVRPDADQMTKIPFFDDVGAVT
                     LQYFDTLFQRDNLQKSQFFKGLPKVLPKLPKRVIVQRILPCLTSEFVNPDMVPFVLPN
                     VLLIAEECTKEEYVKLILPELGPVFKQQEPIQILLIFLQKMDLLLTKTPPDEIKNSVL
                     PMVYRALEAPSIQIQELCLNIIPTFANLIDYPSMKNALIPRIKNACLQTSSLAVRVNS
                     LVCLGKILEYLDKWFVLDDILPFLQQIPSKEPAVLMGILGIYKCTFTHKKLGITKEQL
                     AGKVLPHLIPLSIENNLNLNQFNSFISVIKEMLNRLESEHKTKLEQLHIMQEQQKSLD
                     IGNQMNVSEEMKVTNIGNQQIDKVFNNIGADLLTGSESENKEDGLQNKHKRASLTLEE
                     KQKLAKEQEQAQKLKSQQPLKPQVHTPVATVKQTKDLTDTLMDNMSSLTSLSVSTPKS
                     SASSTFTSVPSMGIGMMFSTPTDNTKRNLTNGLNANMGFQTSGFNMPVNTNQNFYSSP
                     STVGVTKMTLGTPPTLPNFNALSVPPAGAKQTQQRPTDMSALNNLFGPQKPKVSMNQL
                     SQQKPNQWLNQFVPPQGSPTMGSSVMGTQMNVIGQSAFGMQGNPFFNPQNFAQPPTTM
                     TNSSSASNDLKDLFG"
     exon            822..979
                     /gene="SCYL2"
                     /gene_synonym="AMC4; AMCNACC; CVAK104"
                     /inference="alignment:Splign:2.1.0"
     exon            980..1124
                     /gene="SCYL2"
                     /gene_synonym="AMC4; AMCNACC; CVAK104"
                     /inference="alignment:Splign:2.1.0"
     exon            1125..1274
                     /gene="SCYL2"
                     /gene_synonym="AMC4; AMCNACC; CVAK104"
                     /inference="alignment:Splign:2.1.0"
     exon            1275..1496
                     /gene="SCYL2"
                     /gene_synonym="AMC4; AMCNACC; CVAK104"
                     /inference="alignment:Splign:2.1.0"
     exon            1497..1613
                     /gene="SCYL2"
                     /gene_synonym="AMC4; AMCNACC; CVAK104"
                     /inference="alignment:Splign:2.1.0"
     exon            1614..1739
                     /gene="SCYL2"
                     /gene_synonym="AMC4; AMCNACC; CVAK104"
                     /inference="alignment:Splign:2.1.0"
     exon            1740..1916
                     /gene="SCYL2"
                     /gene_synonym="AMC4; AMCNACC; CVAK104"
                     /inference="alignment:Splign:2.1.0"
     exon            1917..2039
                     /gene="SCYL2"
                     /gene_synonym="AMC4; AMCNACC; CVAK104"
                     /inference="alignment:Splign:2.1.0"
     exon            2040..2153
                     /gene="SCYL2"
                     /gene_synonym="AMC4; AMCNACC; CVAK104"
                     /inference="alignment:Splign:2.1.0"
     exon            2154..2286
                     /gene="SCYL2"
                     /gene_synonym="AMC4; AMCNACC; CVAK104"
                     /inference="alignment:Splign:2.1.0"
     exon            2287..2405
                     /gene="SCYL2"
                     /gene_synonym="AMC4; AMCNACC; CVAK104"
                     /inference="alignment:Splign:2.1.0"
     exon            2406..2506
                     /gene="SCYL2"
                     /gene_synonym="AMC4; AMCNACC; CVAK104"
                     /inference="alignment:Splign:2.1.0"
     exon            2507..2573
                     /gene="SCYL2"
                     /gene_synonym="AMC4; AMCNACC; CVAK104"
                     /inference="alignment:Splign:2.1.0"
     exon            2574..2669
                     /gene="SCYL2"
                     /gene_synonym="AMC4; AMCNACC; CVAK104"
                     /inference="alignment:Splign:2.1.0"
     exon            2670..2789
                     /gene="SCYL2"
                     /gene_synonym="AMC4; AMCNACC; CVAK104"
                     /inference="alignment:Splign:2.1.0"
     exon            2790..5977
                     /gene="SCYL2"
                     /gene_synonym="AMC4; AMCNACC; CVAK104"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4379..4384
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SCYL2"
                     /gene_synonym="AMC4; AMCNACC; CVAK104"
                     /note="hexamer: AATAAA"
     polyA_site      4401
                     /gene="SCYL2"
                     /gene_synonym="AMC4; AMCNACC; CVAK104"
                     /note="major polyA site"
     regulatory      5955..5960
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SCYL2"
                     /gene_synonym="AMC4; AMCNACC; CVAK104"
                     /note="hexamer: AATAAA"
     polyA_site      5977
                     /gene="SCYL2"
                     /gene_synonym="AMC4; AMCNACC; CVAK104"
                     /note="major polyA site"
ORIGIN      
        1 agtctttttc cccctccctt actcttcgtc cccggtccct cccctcccca cccctttcct
       61 tctagctccg acgtttgcgg ccgcgggggc ggcggaggat atggagtaaa gccagagtca
      121 gtggccaggc acgaaggcag agcaggaaca gccaggaggc gtttattagg ggggcggggg
      181 gaaagagccc cagcaccgcc cctcctggaa gaaggaagag gtaagtgacc ggccgccggc
      241 accgaccgac ctccctcacc ggcggctctc tcgcctgggc tcccggagcc ggcgaggagg
      301 gaatggagga ctcgcgcccg ggttaggcct cccagggccg ctcaggctgg tgggtgttgc
      361 ctggtgacgg gcctgccggc ggccggccgg gcgatcggcg gtcggcgccc gcgcaaagcg
      421 gggctggacg agcagcgagc tccggggagc ggatccgaga gggccgagtc ctcgaaagag
      481 gccttgaggc gacgggagac ccgggatcga agtcagctgc cggagggaga gccccccatg
      541 ccggctcgag agctcgggtt tcggtggtgg agaacgtagt acctttcggg gacattggac
      601 actactctag gaccgggtaa ctataactac ccaatattgc agccatggag tccatgctta
      661 ataaattgaa gagtactgtt acaaaagtaa cagctgatgt cactagtgct gtaatgggaa
      721 atcctgtcac tagagaattt gatgttggtc gacacattgc cagtggtggc aatgggctag
      781 cttggaagat ttttaatggc acaaaaaagt caacaaagca ggaagtggca gtttttgtct
      841 ttgataaaaa actgattgac aagtatcaaa aatttgaaaa ggatcaaatc attgattctc
      901 taaaacgagg agtccaacag ttaactcggc ttcgacaccc tcgacttctt actgtccagc
      961 atcctttaga agaatccagg gattgcttgg cattttgtac agaaccagtt tttgccagtt
     1021 tagccaatgt tcttggtaac tgggaaaatc taccttcccc tatatctcca gacattaagg
     1081 attataaact ttatgatgta gaaaccaaat atggtttgct tcaggtttct gaaggattgt
     1141 cattcttgca tagcagtgtg aaaatggtgc atggaaatat cactcctgaa aatataattt
     1201 tgaataaaag tggagcctgg aaaataatgg gttttgattt ttgtgtatca tcaaccaatc
     1261 cttctgaaca agagcctaaa tttccttgta aagaatggga cccaaattta ccttcattgt
     1321 gtcttccaaa tcctgaatat ttggctcctg aatacatact ttctgtgagc tgtgaaacag
     1381 ccagtgatat gtattcttta ggaactgtta tgtatgctgt atttaataaa gggaaaccta
     1441 tatttgaagt caacaagcaa gatatttaca agagtttcag taggcagttg gatcagttga
     1501 gtcgtttagg atctagttca cttacaaata tacctgagga agttcgtgaa catgtaaagc
     1561 tactgttaaa tgtaactccg actgtaagac cagatgcaga tcaaatgaca aagattccct
     1621 tctttgatga tgttggtgca gtaacactgc aatattttga taccttattc caaagagata
     1681 atcttcagaa atcacagttt ttcaaaggac tgccaaaggt tctaccaaaa ctgcccaagc
     1741 gtgtcattgt gcagagaatt ttgccttgtt tgacttcaga atttgtaaac cctgacatgg
     1801 taccttttgt tttgcccaat gttctactta ttgctgagga atgcaccaaa gaagaatatg
     1861 tcaaattaat tcttcctgaa cttggccctg tgtttaagca gcaggagcca atccagattt
     1921 tgttaatttt cctacaaaaa atggatttgc tactaaccaa aacccctcct gatgagataa
     1981 agaacagtgt tctacccatg gtttacagag cactagaagc tccttccatt cagatccagg
     2041 agctctgtct aaacatcatt ccaacctttg caaatcttat agactaccca tccatgaaaa
     2101 acgctttgat accaagaatt aaaaatgctt gtctacaaac atcttccctt gcggttcgtg
     2161 taaattcatt agtgtgctta ggaaagattt tggaatactt ggataagtgg tttgtacttg
     2221 atgatatcct acccttctta caacaaattc catccaagga acctgcggtc ctcatgggaa
     2281 ttttaggtat ttacaaatgt acttttactc ataagaagtt gggaatcacc aaagagcagc
     2341 tggccggaaa agtgttgcct catcttattc ccctgagtat tgaaaacaat cttaatctta
     2401 atcagttcaa ttctttcatt tccgtcataa aagaaatgct taatagattg gagtctgaac
     2461 ataagactaa actggagcaa cttcatataa tgcaagaaca gcagaaatct ttggatatag
     2521 gaaatcaaat gaatgtttct gaggagatga aagttacaaa tattgggaat cagcaaattg
     2581 acaaagtttt taacaacatt ggagcagacc ttctgactgg cagtgagtcc gaaaataaag
     2641 aggacgggtt acagaataaa cataaaagag catcacttac acttgaagaa aaacaaaaat
     2701 tagcaaaaga acaagagcag gcacagaagc tgaaaagcca gcagcctctt aaaccccaag
     2761 tgcacacacc tgttgctact gttaaacaga ctaaggactt gacagacaca ctgatggata
     2821 atatgtcatc cttgaccagc ctttctgtta gtacccctaa atcttctgct tcaagtactt
     2881 tcacttctgt tccttccatg ggcattggta tgatgttttc tacaccaact gataatacaa
     2941 agagaaattt gacaaatggc ctaaatgcca atatgggctt tcagacttca ggattcaaca
     3001 tgcccgttaa tacaaaccag aacttctaca gtagtccaag cacagttgga gtgaccaaga
     3061 tgactctggg aacacctccc actttgccaa acttcaatgc tttgagtgtt cctcctgctg
     3121 gtgcaaagca gacccaacaa agacccacag atatgtctgc ccttaataat ctctttggcc
     3181 ctcagaaacc caaagttagc atgaaccagt tatcacaaca gaaaccaaat cagtggctta
     3241 atcagtttgt acctcctcaa ggttctccaa ctatgggcag ttcagtaatg gggacacaga
     3301 tgaacgtgat aggacaatct gcttttggta tgcagggtaa tcctttcttt aacccacaga
     3361 actttgcaca gccaccaact actatgacca atagcagttc agctagcaat gatttaaaag
     3421 atctttttgg gtgaggtgtc ttacttctat tttgaaggat tatttcagtt tcaatcatgg
     3481 gtgagctgat ttacatcttt atatagttgg cttggaggaa gtacttctat gggaaagtga
     3541 acagttctgt gacaggaaac atctctgtcc atgccagcat agtagttgta tggacttcta
     3601 accagttgag ttttttaaag cattgaggat tttttcctct taccaactcc tcttcaggtt
     3661 tttaaagacc cagcccttcc caatctcaaa gagaaaaagg aaactgagtt atcttgaata
     3721 acataacttt ttaatcaaat gtttattttg gcttgtggat cttggtgtta tttaaaaaat
     3781 tgaggtgatg gtcattgcaa gctcatctat taagtactat atggtacaca gtctatgagt
     3841 cattagtctt cattttaata tgtaaaaaat cttgatgctg tattgatttg tttgcattta
     3901 agatgacagt gagaaaatga taagcataaa gagaagtatc aggttatttg ctttttccaa
     3961 acttttcaga tgaactattg tttagtacag agactgagca aatactacaa aattcaactt
     4021 aaccttcatt tcattggttt aaatgcgtta ttaaccatct taagtgcaaa ctaatcattg
     4081 taaattatat tttagcatgg tctgcctcaa atagtaatgt atttttctgc attcacttgg
     4141 atatatttag aatcactttt ttcctcctgt atcaaggaag aggtatgtgc tgatttgttt
     4201 ggatatttga caaggcactc tgatgtgact tccctgacta ctaccttcat atttcatttc
     4261 aaattcaaac ttctgaggtt gcagcatata tgaattgcat tttcaaaaga agatttgtaa
     4321 gaattaaact atatttatga gtaaactttt gaggtttctg ctgtattgtt tcaaatgtaa
     4381 taaactttac ttctgtaaaa attgagcagt tgtatcttct gaccaccaac agattttcag
     4441 cttgccatga tagtctgacc tcattaatta ctgctactga agttcaattt ttttctagga
     4501 attttaggaa ccttttgttt aaatatttta atttctatta gccattttta ggaaggaaag
     4561 aatcaattct cttaacagga aacatgcttt atttttcaaa acctttctct gatatttttc
     4621 tttaatttgc tgattattca accacagagc cttatgctat aaatgtcatt tgtattttaa
     4681 aaaataatat tccactcata aaactttaaa accatctttc aacgaactat atatgtatta
     4741 tagttgctgc catagagttg atggttttta attatctgga accagcaatc atttaaaata
     4801 aaccatatta agtttagtat gctggtattg tttattcatt ttatatgaat attcattgaa
     4861 aatatataca caatatatgt aatacacagc acttgattac aaaatgtaat ttaattatat
     4921 tattgctggc agcattcagt taagagggta ctttaaaaaa tagaagtcag ctttcacatc
     4981 tgatttctgt atgggctgta cttggttaac ttgattttag aaaaaggact aacagaattg
     5041 ctaaagaaat gcatccaata aatgaaaaac agtaggaaga tcaaatgttt ttgtcaaata
     5101 tattcacaac ttgaccagat tagctgtcct gtttgtaatg caatattaat atgtcttttg
     5161 ggaaaaagcc tacatatgga ataaaataag tattgaagaa tttttctttg taacaattta
     5221 gtagtcactg tttattgaga aattgttttt tattttgtaa aataacatga tgttagtgtt
     5281 gaactcttaa acagaaagaa agcttaatat aacagcttat agaacttgaa ctactaaata
     5341 tgaaaataag tcatttgaaa aaaatacagt atgtaaaatt tgttcattcg ttgaggtaat
     5401 ggtgctatgt ttttacaaaa ttgttcctac accttttttc tacttcaggt attttatttc
     5461 aaccatttcc atcaattgaa ctgttaccat tgcctttttc tgttgagaaa ttgcctctga
     5521 aaaatagtgc tatttttcag cttaagtgtt cttaagtgaa tgaaattttc aaagtactag
     5581 atcaccttaa aattatttca cgtactgaag acaattaagt ccgttatgtt tagagtagaa
     5641 aatgtttagg ttaaagagca tctgtcaaca gaatctacaa aaaagattcc cttgcatttg
     5701 aattagttct ctattctcct attgctaaat gtgtgatata tagagaggat gtataaaagg
     5761 aaatggaaat agactatgta cttgtctggt ttttgtttgt ttttattttg gaatgcttat
     5821 aagcctcctt tacactgaat aaggaagtag tttttgtttt cttttgacct gtaaaatacc
     5881 tcacatggtt gtttttacac atgaaagaaa aaggtatatg cgaacatacc tgatatcaag
     5941 aggagtatgc accaaataaa ttttagcttt gataaaa
//
