LOCUS       NM_152753               7819 bp    mRNA    linear   PRI 15-OCT-2024
DEFINITION  Homo sapiens signal peptide, CUB domain and EGF like domain
            containing 3 (SCUBE3), transcript variant 1, mRNA.
ACCESSION   NM_152753
VERSION     NM_152753.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7819)
  AUTHORS   Ali Assar,M.F., Abd El Gayed,E.M., Abd El-Hamid Ewis,A.S.,
            Zaid,A.B. and Abd Allah Mahmoud Fouda,E.
  TITLE     Biochemical study of ZNF76 rs10947540 and SCUBE3 rs1888822 single
            nucleotide polymorphisms in the Egyptian patients with systemic
            lupus Erythematosus
  JOURNAL   J Immunoassay Immunochem 45 (5), 415-431 (2024)
   PUBMED   38982741
  REMARK    GeneRIF: Biochemical study of ZNF76 rs10947540 and SCUBE3 rs1888822
            single nucleotide polymorphisms in the Egyptian patients with
            systemic lupus Erythematosus.
REFERENCE   2  (bases 1 to 7819)
  AUTHORS   Fu,H. and Zhao,Q.
  TITLE     CircSCUBE3 promoted ferroptosis to inhibit lung adenocarcinoma
            progression
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 70 (2), 161-168 (2024)
   PUBMED   38430026
  REMARK    GeneRIF: CircSCUBE3 promoted ferroptosis to inhibit lung
            adenocarcinoma progression.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 7819)
  AUTHORS   Wang,Z., Chen,C., Zhang,J., He,J., Zhang,L., Wu,J. and Tian,Z.
  TITLE     Epithelium-derived SCUBE3 promotes polarized odontoblastic
            differentiation of dental mesenchymal stem cells and pulp
            regeneration
  JOURNAL   Stem Cell Res Ther 14 (1), 130 (2023)
   PUBMED   37189178
  REMARK    GeneRIF: Epithelium-derived SCUBE3 promotes polarized odontoblastic
            differentiation of dental mesenchymal stem cells and pulp
            regeneration.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 7819)
  AUTHORS   Hu,F., Fong,K.O., Cheung,M.P.L., Liu,J.A., Liang,R., Li,T.W.,
            Sharma,R., Ip,P.P., Yang,X. and Cheung,M.
  TITLE     DEPDC1B Promotes Melanoma Angiogenesis and Metastasis through
            Sequestration of Ubiquitin Ligase CDC16 to Stabilize Secreted
            SCUBE3
  JOURNAL   Adv Sci (Weinh) 9 (10), e2105226 (2022)
   PUBMED   35088579
  REMARK    GeneRIF: DEPDC1B Promotes Melanoma Angiogenesis and Metastasis
            through Sequestration of Ubiquitin Ligase CDC16 to Stabilize
            Secreted SCUBE3.
REFERENCE   5  (bases 1 to 7819)
  AUTHORS   Yang,M., Guo,M., Hu,Y. and Jiang,Y.
  TITLE     Scube regulates synovial angiogenesis-related signaling
  JOURNAL   Med Hypotheses 81 (5), 948-953 (2013)
   PUBMED   24084593
  REMARK    GeneRIF: Scube3 facilitates both TGFbeta and Hh signalling within
            an overall role of facillating inflammatory angiogenesis.
REFERENCE   6  (bases 1 to 7819)
  AUTHORS   Carvalho,A.Y., Bishop,K.S., Han,D.Y., Ellett,S., Jesuthasan,A.,
            Lam,W.J. and Ferguson,L.R.
  TITLE     The role of Vitamin D level and related single nucleotide
            polymorphisms in Crohn's disease
  JOURNAL   Nutrients 5 (10), 3898-3909 (2013)
   PUBMED   24084050
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 7819)
  AUTHORS   Chou,C.H., Cheng,Y.F., Siow,T.Y., Kumar,A., Peck,K. and Chang,C.
  TITLE     SCUBE3 regulation of early lung cancer angiogenesis and metastatic
            progression
  JOURNAL   Clin Exp Metastasis 30 (6), 741-752 (2013)
   PUBMED   23420440
  REMARK    GeneRIF: results show that SCUBE3 knockdown was associated with
            lower vascular permeability in the tumor and effectively inhibited
            the metastatic potential of non-small-cell lung carcinoma
REFERENCE   8  (bases 1 to 7819)
  AUTHORS   Wu,Y.Y., Peck,K., Chang,Y.L., Pan,S.H., Cheng,Y.F., Lin,J.C.,
            Yang,R.B., Hong,T.M. and Yang,P.C.
  TITLE     SCUBE3 is an endogenous TGF-beta receptor ligand and regulates the
            epithelial-mesenchymal transition in lung cancer
  JOURNAL   Oncogene 30 (34), 3682-3693 (2011)
   PUBMED   21441952
  REMARK    GeneRIF: in lung cancer cells, SCUBE3 could serve as an endogenous
            autocrine and paracrine ligand of TGF-beta type II receptor, which
            could regulate TGF-beta receptor signaling and modulate EMT and
            cancer progression
REFERENCE   9  (bases 1 to 7819)
  AUTHORS   Morris,M.R., Ricketts,C.J., Gentle,D., McRonald,F., Carli,N.,
            Khalili,H., Brown,M., Kishida,T., Yao,M., Banks,R.E., Clarke,N.,
            Latif,F. and Maher,E.R.
  TITLE     Genome-wide methylation analysis identifies epigenetically
            inactivated candidate tumour suppressor genes in renal cell
            carcinoma
  JOURNAL   Oncogene 30 (12), 1390-1401 (2011)
   PUBMED   21132003
REFERENCE   10 (bases 1 to 7819)
  AUTHORS   Wu,B.T., Su,Y.H., Tsai,M.T., Wasserman,S.M., Topper,J.N. and
            Yang,R.B.
  TITLE     A novel secreted, cell-surface glycoprotein containing multiple
            epidermal growth factor-like repeats and one CUB domain is highly
            expressed in primary osteoblasts and bones
  JOURNAL   J Biol Chem 279 (36), 37485-37490 (2004)
   PUBMED   15234972
  REMARK    GeneRIF: may act locally and/or distantly through a proteolytic
            mechanism, and may play an important role in bone cell biology
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from Z97832.11, BC052263.1,
            AF452494.1 and CR936873.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_152753.3.
            
            Summary: This gene encodes a member of the signal peptide,
            complement subcomponents C1r/C1s, Uegf, bone morphogenetic
            protein-1 and epidermal growth factor-like domain containing
            protein family. Overexpression of this gene in human embryonic
            kidney cells results in secretion of a glycosylated form of the
            protein that forms oligomers and tethers to the cell surface. This
            gene is upregulated in lung cancer tumor tissue compared to healthy
            tissue and is associated with loss of the epithelial marker
            E-cadherin and with increased expression of vimentin, a mesenchymal
            marker. In addition, the protein encoded by this gene is a
            transforming growth factor beta receptor ligand, and when secreted
            by cancer cells, it can be cleaved in vitro to release the
            N-terminal epidermal growth factor-like repeat domain and the
            C-terminal complement subcomponents C1r/C1s domain. Both the full
            length protein and C-terminal fragment can bind to the transforming
            growth factor beta type II receptor to promote the
            epithelial-mesenchymal transition and tumor angiogenesis.
            Alternative splicing results in multiple transcript variants.
            [provided by RefSeq, Dec 2014].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF452494.1, AY639608.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMEA2144120
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000274938.8/ ENSP00000274938.7
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-106               Z97832.11          5064-5169
            107-233             BC052263.1         1-127
            234-234             Z97832.11          5297-5297
            235-457             BC052263.1         129-351
            458-3045            AF452494.1         1-2588
            3046-3046           Z97832.11          36517-36517
            3047-5572           AF452494.1         2590-5115
            5573-5907           CR936873.1         5169-5503
            5908-5912           Z97832.11          42276-42280
            5913-7819           CR936873.1         5506-7412
FEATURES             Location/Qualifiers
     source          1..7819
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6p21.31"
     gene            1..7819
                     /gene="SCUBE3"
                     /gene_synonym="CEGF3; SSFSC2"
                     /note="signal peptide, CUB domain and EGF like domain
                     containing 3"
                     /db_xref="GeneID:222663"
                     /db_xref="HGNC:HGNC:13655"
                     /db_xref="MIM:614708"
     exon            1..548
                     /gene="SCUBE3"
                     /gene_synonym="CEGF3; SSFSC2"
                     /inference="alignment:Splign:2.1.0"
     CDS             464..3445
                     /gene="SCUBE3"
                     /gene_synonym="CEGF3; SSFSC2"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; CUB domain and EGF-like repeat containing 3;
                     signal peptide, CUB and EGF-like domain-containing protein
                     3"
                     /codon_start=1
                     /product="signal peptide, CUB and EGF-like
                     domain-containing protein 3 isoform 1 precursor"
                     /protein_id="NP_689966.2"
                     /db_xref="CCDS:CCDS4800.1"
                     /db_xref="GeneID:222663"
                     /db_xref="HGNC:HGNC:13655"
                     /db_xref="MIM:614708"
                     /translation="MGSGRVPGLCLLVLLVHARAAQYSKAAQDVDECVEGTDNCHIDA
                     ICQNTPRSYKCICKSGYTGDGKHCKDVDECEREDNAGCVHDCVNIPGNYRCTCYDGFH
                     LAHDGHNCLDVDECAEGNGGCQQSCVNMMGSYECHCREGFFLSDNQHTCIQRPEEGMN
                     CMNKNHGCAHICRETPKGGIACECRPGFELTKNQRDCKLTCNYGNGGCQHTCDDTEQG
                     PRCGCHIKFVLHTDGKTCIETCAVNNGGCDSKCHDAATGVHCTCPVGFMLQPDRKTCK
                     DIDECRLNNGGCDHICRNTVGSFECSCKKGYKLLINERNCQDIDECSFDRTCDHICVN
                     TPGSFQCLCHRGYLLYGITHCGDVDECSINRGGCRFGCINTPGSYQCTCPAGQGRLHW
                     NGKDCTEPLKCQGSPGASKAMLSCNRSGKKDTCALTCPSRARFLPESENGFTVSCGTP
                     SPRAAPARAGHNGNSTNSNHCHEAAVLSIKQRASFKIKDAKCRLHLRNKGKTEEAGRI
                     TGPGGAPCSECQVTFIHLKCDSSRKGKGRRARTPPGKEVTRLTLELEAEVRAEETTAS
                     CGLPCLRQRMERRLKGSLKMLRKSINQDRFLLRLAGLDYELAHKPGLVAGERAEPMES
                     CRPGQHRAGTKCVSCPQGTYYHGQTEQCVPCPAGTFQEREGQLSCDLCPGSDAHGPLG
                     ATNVTTCAGQCPPGQHSVDGFKPCQPCPRGTYQPEAGRTLCFPCGGGLTTKHEGAISF
                     QDCDTKVQCSPGHYYNTSIHRCIRCAMGSYQPDFRQNFCSRCPGNTSTDFDGSTSVAQ
                     CKNRQCGGELGEFTGYIESPNYPGNYPAGVECIWNINPPPKRKILIVVPEIFLPSEDE
                     CGDVLVMRKNSSPSSITTYETCQTYERPIAFTARSRKLWINFKTSEANSARGFQIPYV
                     TYDEDYEQLVEDIVRDGRLYASENHQEILKDKKLIKAFFEVLAHPQNYFKYTEKHKEM
                     LPKSFIKLLRSKVSSFLRPYK"
     exon            549..671
                     /gene="SCUBE3"
                     /gene_synonym="CEGF3; SSFSC2"
                     /inference="alignment:Splign:2.1.0"
     exon            672..797
                     /gene="SCUBE3"
                     /gene_synonym="CEGF3; SSFSC2"
                     /inference="alignment:Splign:2.1.0"
     exon            798..932
                     /gene="SCUBE3"
                     /gene_synonym="CEGF3; SSFSC2"
                     /inference="alignment:Splign:2.1.0"
     exon            933..1058
                     /gene="SCUBE3"
                     /gene_synonym="CEGF3; SSFSC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1059..1175
                     /gene="SCUBE3"
                     /gene_synonym="CEGF3; SSFSC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1176..1292
                     /gene="SCUBE3"
                     /gene_synonym="CEGF3; SSFSC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1293..1415
                     /gene="SCUBE3"
                     /gene_synonym="CEGF3; SSFSC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1416..1532
                     /gene="SCUBE3"
                     /gene_synonym="CEGF3; SSFSC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1533..1658
                     /gene="SCUBE3"
                     /gene_synonym="CEGF3; SSFSC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1659..1775
                     /gene="SCUBE3"
                     /gene_synonym="CEGF3; SSFSC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1776..1880
                     /gene="SCUBE3"
                     /gene_synonym="CEGF3; SSFSC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1881..1997
                     /gene="SCUBE3"
                     /gene_synonym="CEGF3; SSFSC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1998..2156
                     /gene="SCUBE3"
                     /gene_synonym="CEGF3; SSFSC2"
                     /inference="alignment:Splign:2.1.0"
     exon            2157..2372
                     /gene="SCUBE3"
                     /gene_synonym="CEGF3; SSFSC2"
                     /inference="alignment:Splign:2.1.0"
     exon            2373..2534
                     /gene="SCUBE3"
                     /gene_synonym="CEGF3; SSFSC2"
                     /inference="alignment:Splign:2.1.0"
     exon            2535..2702
                     /gene="SCUBE3"
                     /gene_synonym="CEGF3; SSFSC2"
                     /inference="alignment:Splign:2.1.0"
     exon            2703..2864
                     /gene="SCUBE3"
                     /gene_synonym="CEGF3; SSFSC2"
                     /inference="alignment:Splign:2.1.0"
     exon            2865..3062
                     /gene="SCUBE3"
                     /gene_synonym="CEGF3; SSFSC2"
                     /inference="alignment:Splign:2.1.0"
     exon            3063..3215
                     /gene="SCUBE3"
                     /gene_synonym="CEGF3; SSFSC2"
                     /inference="alignment:Splign:2.1.0"
     exon            3216..3295
                     /gene="SCUBE3"
                     /gene_synonym="CEGF3; SSFSC2"
                     /inference="alignment:Splign:2.1.0"
     exon            3296..7819
                     /gene="SCUBE3"
                     /gene_synonym="CEGF3; SSFSC2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7794..7799
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SCUBE3"
                     /gene_synonym="CEGF3; SSFSC2"
                     /note="hexamer: ATTAAA"
     polyA_site      7819
                     /gene="SCUBE3"
                     /gene_synonym="CEGF3; SSFSC2"
                     /note="major polyA site"
ORIGIN      
        1 gcgagagagg aagaaagaga ggcagaaagg gcgtgtttct ggcgctgcgt ttcccctccc
       61 ctttctcagg tccttcgctc gggctctgcg cgctctccgg ctgcagctct ctcccggcga
      121 agctgggaat tgggtgggat tacacggagc agccccgccg ccgccgctgg cagaggccgg
      181 cttggagagg gcgggggttc ccctccgtca gtcgcccctg gcgcccctcg cctcgtcgca
      241 ctctccgcct cgctctcccc gacgtccggc caggaggagc cggtagcatc gggagcctcg
      301 cgccgagggc gccgcggtcc gcgccccgcg actgcagccc ccggcctggc cccggcgggg
      361 cgccccctcc cctccccctc ctgcgagctg ggatccggcc ggcttccgcc ctcccctggc
      421 cgcgagaccg gccccggcgg ctgggccgcc agtagctcca gccatgggct cggggcgcgt
      481 acccgggctc tgcctgcttg tcctgctggt ccacgcccgc gccgcccagt acagcaaagc
      541 cgcgcaagat gtggatgagt gtgtggaggg gactgacaac tgccacatcg atgctatctg
      601 ccagaacacc ccgaggtcat acaagtgcat ctgcaagtct ggctacacag gggacggcaa
      661 acactgcaaa gacgtggatg agtgcgagcg agaggataat gcaggttgtg tgcatgactg
      721 tgtcaacatc cctggcaatt accggtgtac ctgctatgat ggattccacc tggcacatga
      781 cggacacaac tgtctggatg tggacgagtg tgccgagggc aacggcggct gtcagcagag
      841 ctgtgtcaac atgatgggca gctatgagtg ccactgccgg gaaggcttct tcctcagcga
      901 caaccagcat acctgtatcc agcggccaga agaaggaatg aattgcatga acaagaacca
      961 cggctgtgcc cacatttgcc gggagacacc caaggggggt attgcctgtg aatgccgtcc
     1021 tggctttgag cttaccaaga accaacggga ctgtaaattg acatgcaact atggtaacgg
     1081 cggctgccag cacacgtgtg atgacacaga gcagggtccc cggtgcggct gccatatcaa
     1141 gtttgtgctc cataccgacg ggaagacatg catcgagacc tgtgctgtca acaacggggg
     1201 ctgtgacagt aagtgccatg atgcagcgac tggtgtccac tgcacctgcc ctgtgggctt
     1261 catgctgcag ccagacagga agacgtgcaa agatatagat gagtgccgct taaacaacgg
     1321 gggctgtgac catatttgcc gcaacacagt gggcagcttc gaatgcagtt gcaagaaagg
     1381 ctataagctt ctcatcaatg agaggaactg ccaggatata gacgagtgtt cctttgatcg
     1441 aacctgtgac cacatatgtg tcaacacacc aggaagcttc cagtgtctct gccatcgtgg
     1501 ctacctgttg tatggtatca cccactgtgg ggatgtggat gaatgcagca tcaaccgggg
     1561 aggttgccgc tttggctgca tcaacactcc tggcagctac cagtgtacct gcccagcagg
     1621 ccagggtcgg ctgcactgga atggcaaaga ttgcacagag ccactgaagt gtcagggcag
     1681 tcctggggcc tcgaaagcca tgctcagctg caaccggtct ggcaagaagg acacctgtgc
     1741 cctgacctgt ccctccaggg cccgattttt gccagagtct gagaatggct tcacggtgag
     1801 ctgtgggacc cccagcccca gggctgctcc agcccgagct ggccacaatg ggaacagcac
     1861 caactccaac cactgccatg aggctgcagt gctgtccatt aaacaacggg cctccttcaa
     1921 gatcaaggat gccaaatgcc gtttgcacct gcgaaacaaa ggcaaaacag aggaggctgg
     1981 cagaatcaca gggccaggtg gtgccccctg ctctgaatgc caggtcacct tcatccacct
     2041 taagtgtgac tcctctcgga agggcaaggg ccgacgggcc cggacccctc caggcaaaga
     2101 ggtcacaagg ctcaccctgg aactggaggc agaggtcaga gccgaagaaa ccacagccag
     2161 ctgtgggctg ccctgcctcc gacagcgaat ggaacggcgg ctgaaaggat ccctgaagat
     2221 gctcagaaag tccatcaacc aggaccgctt cctgctgcgc ctggcaggcc ttgattatga
     2281 gctggcccac aagccgggcc tggtagccgg ggagcgagca gagccgatgg agtcctgtag
     2341 gcccgggcag caccgtgctg ggaccaagtg tgtcagctgc ccgcagggaa cgtattacca
     2401 cggccagacg gagcagtgtg tgccatgccc agcgggcacc ttccaggaga gagaagggca
     2461 gctctcctgc gacctttgcc ctgggagtga tgcccacggg cctcttggag ccaccaacgt
     2521 caccacgtgt gcaggtcagt gcccacctgg ccaacactct gtagatgggt tcaagccctg
     2581 tcagccatgc ccacgtggca cctaccaacc tgaagcagga cggaccctat gcttcccttg
     2641 tggtgggggc ctcaccacca agcatgaagg ggccatttcc ttccaagact gtgacaccaa
     2701 agtccagtgc tccccagggc actactacaa caccagcatc caccgctgta ttcgctgtgc
     2761 catgggctcc tatcagcccg acttccgtca gaacttctgc agccgctgtc caggaaacac
     2821 aagcacagac tttgatggct ctaccagtgt ggcccaatgc aagaatcgtc agtgtggtgg
     2881 ggagctgggt gagttcactg gctatattga gtcccccaac tacccgggca actacccagc
     2941 tggtgtggag tgcatctgga acatcaaccc cccacccaag cgcaagatcc ttatcgtggt
     3001 accagagatc ttcctgccat ctgaggatga gtgtggggac gtcctcgtca tgagaaagaa
     3061 ctcatcccca tcctccatta ccacttatga gacctgccag acctacgagc gtcccattgc
     3121 cttcactgcc cgttccagga agctctggat caacttcaag acaagcgagg ccaacagcgc
     3181 ccgtggcttc cagattccct atgttaccta tgatgaggac tatgagcagc tggtagaaga
     3241 cattgtgcga gatggccggc tctatgcctc tgaaaaccac caggagattt taaaggacaa
     3301 gaagctcatc aaggccttct ttgaggtgct agcccacccc cagaactact tcaagtacac
     3361 agagaaacac aaggagatgc tgccaaaatc cttcatcaag ctgctccgct ccaaagtttc
     3421 cagcttcctg aggccctaca aatagtaacc ctaggctcag agacccaatt ttttaagccc
     3481 ccagactcct tagccctcag agccggcagc cccctaccct cagacaagga actctctcct
     3541 ctctttttgg agggaaaaaa aaaatatcac tacacaaacc aggcactctc cctttctgtc
     3601 tttctagttt cctttccttg tctctctctg cctgcctctc tactgttccc ccttttctaa
     3661 cacactacct agaaaagcca ttcagtactg gctctagtcc ccgtgagatg taaagaaaca
     3721 gtacagcccc ttccactgcc cattttacca gctcacattc ccgaccccat cagcttggaa
     3781 gggtgctaga ggcccatcaa ggaagtgggt ctggtgggaa acggggaggg gaaagaaggg
     3841 cttctgccat tatagggttg tgccttgcta gtcaggggcc aaaatgtccc ctggctctgc
     3901 tccctagggt gattctaaca gcccagggtc ctgccaaaga agcctttgat ttacaggctt
     3961 aatgccagca ccagtcctct ggggcacatg gtttgagctc tggacttccc acatggccag
     4021 ctttcttgtc tatacagatc ctctctttct ttccctacgt ctgcctgggg tctactccat
     4081 aagggtttac aaatggccca caacactgag ttagtggaca ccggctaaat gaggaagagc
     4141 agcaggcatt gtcatggtga atgccccgct gtagctccct gagagaaaga ctgtaactct
     4201 gcaggacaga aacaaggttt taaagcattg ccaaaaaaaa gaaaacagaa agaaaaaatg
     4261 tatcatctaa aggactagac acagaacaat tggaagtcaa cttcaaacac taatcccttt
     4321 tcttgtcttc cctggcccag ccacctcctc agccccatgt gatgctccct gggggagccc
     4381 tactcccctt gctacatgtt gtccttaaac atggttattg acctgaagcc agcctaggcc
     4441 ttgccctaca gttgtttttc ccttgtagcc ccagctggct tgtgggcttc accaaagagg
     4501 accccactct gaagccagcc tggagccacc tacctctggc ctcaggctgt gggcagcaaa
     4561 aggaatgtgt gtgcacttgg cgagcctcct gcccaccctg tccacaccta ataagtgcaa
     4621 tcattttgag tctttctatg ttgtctagac ggaggggttt ttgttttctg ggtttgtttt
     4681 ttgtttttgt ttcttcttcc tctattagca aaaccctatt tatagctgcc caagagaaaa
     4741 gagtgtatgt ttggagtgga agaaaatcgg ttttgaatct catgaacctt gagtgctgga
     4801 gcatctgatc tgtctctatg ccaccactgg ccacctagag cccttggctg tggtaatcca
     4861 gggtaattgc gcagaggcat ctgatgtgta ggaaagtaat tctggggatt tgatggagca
     4921 gaaaggagag agacctatgt ttgctaaacc aatcttgcta tccctatgcc tctccatgga
     4981 gtcagtgtgg acctcatgat tatagaggcc aatggaactg gtcagtgatt ctctacccca
     5041 agtagggaaa acctccatct tttccctgtc ttcatcctgt tatcccctgg tgtacacatg
     5101 gaagaggacc aggacatacc acctggggat gggctgactc agggtcccga ggccagagac
     5161 aaagctgtgg gagccaagaa taagacagaa ggcatcagat atttgcctgg ctctggtcac
     5221 ttccctttga agttgacttc aagcttagct tcctcaacta ctgtttttca gaaggaagaa
     5281 agaatcaaat ggaagaagaa tcaagtccat gcccaagccc aaagcctgca cacattctgc
     5341 ccttaatcca agtgttgcct ataaaattat tttctgtttt ctcactctag ttcccccaaa
     5401 gctgtagtcc caatcaatca aaggctacca actgagtcct caggttcatt tctttttttt
     5461 ttttttttct ttttaactct cctggtaact cacagaacag ctcaggttca tttctaggtg
     5521 actgtcctag gctactgctc ccccaggaga ctcaacacta aataaaggag tctgactccc
     5581 tgccctccca tcagtagcat tcatcagcca aaacggccat agcagtaggt agccaatgac
     5641 acagctagat tccctgacaa ccattagcca acatcatggt ccttgtgggt agagtgtctg
     5701 gctacctttg tctttctggg tcaaggaggg ccactgtaaa gatgcaaaat ggctgcttcc
     5761 aaacaaggga aatggtcatt tagtatgagt gtctctgtac ttcccaggaa gcaccatgaa
     5821 aactgcttta atcaactctg actatcctaa ttctccacca gaagctagga taactttctt
     5881 tctttctttc tttttttttt tttttttttt tgagatggag tctcgctctg tcacccaggc
     5941 tggagtgtaa tggtgctatc tcggcctact gcaacctccg cctcccaggc tcaagcaatt
     6001 ctgcctcggc ctcccaagta gctgggatta caggcatgcg ccaccgcgcc cagctaattt
     6061 ttgtattttt agtagagatg gggtttcacc atgttgcccg ggctggtctt gaactcttga
     6121 cctcaggtga tccacccgcc tcagtctccc aaagtgctgg gattacaggc atgagccacc
     6181 gcgcccagcc taggataact ttctgattcc tctctgccag ccgttttgcg tctcttggaa
     6241 agccaaacgg tgaccatgct tcttaattta tgccttcagg gtctggcttc tcctttctcc
     6301 cttcctttcc tgtcacacca tgcatacata catacaaata cacatccttc aaccatttat
     6361 tccatggctt atgagacctg caaatgagtt ccacagtacg gaaggcatag accactaggc
     6421 ttcttaattg atgtcaaggc agatcttggt gagcaggtaa aaacctgcta ttgtccacca
     6481 agtttaattt aggtcctcca agttggaggg ttaagaaccc aggcaaagct gctgctgaat
     6541 ctatggagga ggctggccct gggacatcaa actaggggtt aactggattg aatgaggagt
     6601 caacgctcag ggacattcta ggtctttaca ggtcagacag aagagaggtt tttaccattg
     6661 gagggaaatg gaaagatggt ataaatagga tcccttcatg aagcaaccca gaggcctctc
     6721 tgcagcgtgt agggtgtggg gcgacagtat tgtggggctt ccattcatct tagtacaaaa
     6781 cctcacctgc tctgagcctg gaaatgggag ggcttcacgc accaagtaat gcacaccaga
     6841 aaggcactta tatcctcagc aacatggcag ttcctcttta cttctctgcc tccctctttc
     6901 tatattatca ggccatgcct attcctacaa cctgagacaa ctcttggagt cagaagaaaa
     6961 ctgaccagat cctggatctg agctgcctgc tccaggccta gaaatcccca aaggctggca
     7021 ctgagctgtg actgctttaa cagcccccaa gatttggtca gtttgaggtg gtggagactc
     7081 agatttgttg ctgaaagttc agtaacacag tcctggtctt tggccctaga gaaacttttt
     7141 atatgagaag tgttctctat atacatgttt gaggtgactc tggaatggat tatgaggtca
     7201 tatctcaaaa tgtcagaaaa cgttatagag cactcgaact tttgtatttg ctgcttaacc
     7261 tcaatattac agccacaaac aaggggtacc aagacaaagt ataactgagc ataagcagaa
     7321 aatgttaacc ctccaggttt ctttcttaag cacaataaaa gtgggagcga acaacacaag
     7381 gatattttta catttgaccc gtctcaaaag tagcacaccc tatccttgtg ccattatttg
     7441 tacaaggaaa tatatgatta gaaggaatag aacccccagt tgtcatcagc ttttttagac
     7501 accacaggtt gtagcagttt gaacaaactg aaaactttat acttctgtgt gagctgaact
     7561 caagtttcag aataatcatc gccatgtggg aggctttttg ttaaatgcag aagaaatttc
     7621 aaaatattgt atttatatct gccttccact gctgccaatt tagtaagcat ctcctataca
     7681 atcgacaata aacagcaaat gatgcagttc atagagtatt ttgcacttgg ggaaaaatat
     7741 gtatctgaat tgtaaaaaga aatgtttgga ttttgtatgt cttttttatt attattaaaa
     7801 tactaaatga aactcctca
//
