LOCUS       NM_173050               9795 bp    mRNA    linear   PRI 01-JUN-2024
DEFINITION  Homo sapiens signal peptide, CUB domain and EGF like domain
            containing 1 (SCUBE1), mRNA.
ACCESSION   NM_173050
VERSION     NM_173050.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9795)
  AUTHORS   Qu,X., Huang,D., Zhou,X. and Ruan,W.
  TITLE     SCUBE1 promotes pulmonary artery smooth muscle cell proliferation
            and migration in acute pulmonary embolism by modulating BMP7
  JOURNAL   PeerJ 12, e16719 (2024)
   PUBMED   38259670
  REMARK    GeneRIF: SCUBE1 promotes pulmonary artery smooth muscle cell
            proliferation and migration in acute pulmonary embolism by
            modulating BMP7.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9795)
  AUTHORS   Ayvaz Celik,H.H., Kuyumcu,M.S., Sirin,F.B., Cirit,M., Korkmaz,S.,
            Erturan,I., Celik,S. and Yildirim,M.
  TITLE     Could SCUBE-1 be a marker for subclinical atherosclerosis other
            than carotid artery intima-media thickness in patients with
            psoriasis?
  JOURNAL   An Bras Dermatol 98 (5), 595-601 (2023)
   PUBMED   37198010
  REMARK    GeneRIF: Could SCUBE-1 be a marker for subclinical atherosclerosis
            other than carotid artery intima-media thickness in patients with
            psoriasis?
REFERENCE   3  (bases 1 to 9795)
  AUTHORS   Xiao,L., Wang,M., Yang,S., Li,S., Huang,Q., Xu,L., Li,Y. and Fu,Y.
  TITLE     The diagnostic potential of plasma SCUBE-1 concentration for
            pulmonary embolism: A pilot study
  JOURNAL   Clin Respir J 17 (4), 263-269 (2023)
   PUBMED   36748401
  REMARK    GeneRIF: The diagnostic potential of plasma SCUBE-1 concentration
            for pulmonary embolism: A pilot study.
REFERENCE   4  (bases 1 to 9795)
  AUTHORS   Yang,M., Liu,Y., Ma,Y. and Wang,W.
  TITLE     Predictive Value of Combined Plasma D-Dimer, SCUBE1, and Right
            Ventricular Tei Index for the Prognosis of Elderly Patients with
            Acute Pulmonary Thromboembolism
  JOURNAL   Rejuvenation Res 26 (1), 32-38 (2023)
   PUBMED   36517978
  REMARK    GeneRIF: Predictive Value of Combined Plasma D-Dimer, SCUBE1, and
            Right Ventricular Tei Index for the Prognosis of Elderly Patients
            with Acute Pulmonary Thromboembolism.
REFERENCE   5  (bases 1 to 9795)
  AUTHORS   Toprak,K., Kaplangoray,M., Palice,A., Tascanov,M.B., Inanir,M.,
            Memioglu,T., Kok,Z., Bicer,A. and Demirbag,R.
  TITLE     SCUBE1 is associated with thrombotic complications, disease
            severity, and in-hospital mortality in COVID-19 patients
  JOURNAL   Thromb Res 220, 100-106 (2022)
   PUBMED   36334396
  REMARK    GeneRIF: SCUBE1 is associated with thrombotic complications,
            disease severity, and in-hospital mortality in COVID-19 patients.
REFERENCE   6  (bases 1 to 9795)
  AUTHORS   Xie,H., Diber,A., Pollock,S., Nemzer,S., Safer,H., Meloon,B.,
            Olson,A., Hwang,J.J., Endress,G.A., Savitsky,K. and Gill-More,R.
  TITLE     Bridging expressed sequence alignments through targeted cDNA
            sequencing
  JOURNAL   Genomics 83 (4), 572-576 (2004)
   PUBMED   15028280
REFERENCE   7  (bases 1 to 9795)
  AUTHORS   Yang,R.B., Ng,C.K., Wasserman,S.M., Colman,S.D., Shenoy,S.,
            Mehraban,F., Komuves,L.G., Tomlinson,J.E. and Topper,J.N.
  TITLE     Identification of a novel family of cell-surface proteins expressed
            in human vascular endothelium
  JOURNAL   J Biol Chem 277 (48), 46364-46373 (2002)
   PUBMED   12270931
  REMARK    GeneRIF: identification as secreted protein that is expressed in
            vascular endothelium and may play important roles in development,
            inflammation, and thrombosis
REFERENCE   8  (bases 1 to 9795)
  AUTHORS   Kumar,S., Connor,J.R., Dodds,R.A., Halsey,W., Van Horn,M., Mao,J.,
            Sathe,G., Mui,P., Agarwal,P., Badger,A.M., Lee,J.C., Gowen,M. and
            Lark,M.W.
  TITLE     Identification and initial characterization of 5000 expressed
            sequenced tags (ESTs) each from adult human normal and
            osteoarthritic cartilage cDNA libraries
  JOURNAL   Osteoarthritis Cartilage 9 (7), 641-653 (2001)
   PUBMED   11597177
REFERENCE   9  (bases 1 to 9795)
  AUTHORS   Grimmond,S., Larder,R., Van Hateren,N., Siggers,P., Hulsebos,T.J.,
            Arkell,R. and Greenfield,A.
  TITLE     Cloning, mapping, and expression analysis of a gene encoding a
            novel mammalian EGF-related protein (SCUBE1)
  JOURNAL   Genomics 70 (1), 74-81 (2000)
   PUBMED   11087664
REFERENCE   10 (bases 1 to 9795)
  AUTHORS   Dunham,I., Shimizu,N., Roe,B.A., Chissoe,S., Hunt,A.R.,
            Collins,J.E., Bruskiewich,R., Beare,D.M., Clamp,M., Smink,L.J.,
            Ainscough,R., Almeida,J.P., Babbage,A., Bagguley,C., Bailey,J.,
            Barlow,K., Bates,K.N., Beasley,O., Bird,C.P., Blakey,S.,
            Bridgeman,A.M., Buck,D., Burgess,J., Burrill,W.D., O'Brien,K.P. et
            al.
  TITLE     The DNA sequence of human chromosome 22
  JOURNAL   Nature 402 (6761), 489-495 (1999)
   PUBMED   10591208
  REMARK    Erratum:[Nature 2000 Apr 20;404(6780):904]
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from Z99756.7 and Z82214.23.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 21, 2019 this sequence version replaced NM_173050.4.
            
            Summary: This gene encodes a cell surface glycoprotein that is a
            member of the SCUBE (signal peptide, CUB domain, EGF (epidermal
            growth factor)-like protein) family. Family members have an
            amino-terminal signal peptide, nine copies of EGF-like repeats and
            a CUB domain at the carboxyl terminus. This protein is expressed in
            platelets and endothelial cells and may play an important role in
            vascular biology. [provided by RefSeq, Oct 2011].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF525689.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000360835.9/ ENSP00000354080.3
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-199               Z99756.7           68336-68534         c
            200-331             Z99756.7           64266-64397         c
            332-460             Z99756.7           45099-45227         c
            461-595             Z99756.7           16208-16342         c
            596-721             Z82214.23          117171-117296       c
            722-838             Z82214.23          112670-112786       c
            839-955             Z82214.23          93289-93405         c
            956-1078            Z82214.23          86204-86326         c
            1079-1195           Z82214.23          83523-83639         c
            1196-1318           Z82214.23          81825-81947         c
            1319-1438           Z82214.23          77548-77667         c
            1439-1543           Z82214.23          77089-77193         c
            1544-1660           Z82214.23          75624-75740         c
            1661-1798           Z82214.23          74901-75038         c
            1799-2002           Z82214.23          72706-72909         c
            2003-2164           Z82214.23          68541-68702         c
            2165-2332           Z82214.23          66876-67043         c
            2333-2494           Z82214.23          65373-65534         c
            2495-2692           Z82214.23          64494-64691         c
            2693-2845           Z82214.23          62523-62675         c
            2846-2925           Z82214.23          61985-62064         c
            2926-9795           Z82214.23          51731-58600         c
FEATURES             Location/Qualifiers
     source          1..9795
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="22"
                     /map="22q13.2"
     gene            1..9795
                     /gene="SCUBE1"
                     /note="signal peptide, CUB domain and EGF like domain
                     containing 1"
                     /db_xref="GeneID:80274"
                     /db_xref="HGNC:HGNC:13441"
                     /db_xref="MIM:611746"
     exon            1..199
                     /gene="SCUBE1"
                     /inference="alignment:Splign:2.1.0"
     CDS             112..3078
                     /gene="SCUBE1"
                     /note="signal peptide-CUB domain-EGF-related 1; signal
                     peptide, CUB and EGF-like domain-containing protein 1;
                     signal peptide, CUB domain, EGF-like 1"
                     /codon_start=1
                     /product="signal peptide, CUB and EGF-like
                     domain-containing protein 1 precursor"
                     /protein_id="NP_766638.2"
                     /db_xref="CCDS:CCDS14048.1"
                     /db_xref="GeneID:80274"
                     /db_xref="HGNC:HGNC:13441"
                     /db_xref="MIM:611746"
                     /translation="MGAAAVRWHLCVLLALGTRGRLAGGSGLPGSVDVDECSEGTDDC
                     HIDAICQNTPKSYKCLCKPGYKGEGKQCEDIDECENDYYNGGCVHECINIPGNYRCTC
                     FDGFMLAHDGHNCLDVDECQDNNGGCQQICVNAMGSYECQCHSGFFLSDNQHTCIHRS
                     NEGMNCMNKDHGCAHICRETPKGGVACDCRPGFDLAQNQKDCTLTCNYGNGGCQHSCE
                     DTDTGPTCGCHQKYALHSDGRTCIETCAVNNGGCDRTCKDTATGVRCSCPVGFTLQPD
                     GKTCKDINECLVNNGGCDHFCRNTVGSFECGCRKGYKLLTDERTCQDIDECSFERTCD
                     HICINSPGSFQCLCHRGYILYGTTHCGDVDECSMSNGSCDQGCVNTKGSYECVCPPGR
                     RLHWNGKDCVETGKCLSRAKTSPRAQLSCSKAGGVESCFLSCPAHTLFVPDSENSYVL
                     SCGVPGPQGKALQKRNGTSSGLGPSCSDAPTTPIKQKARFKIRDAKCHLRPHSQARAK
                     ETARQPLLDHCHVTFVTLKCDSSKKRRRGRKSPSKEVSHITAEFEIETKMEEASDTCE
                     ADCLRKRAEQSLQAAIKTLRKSIGRQQFYVQVSGTEYEVAQRPAKALEGQGACGAGQV
                     LQDSKCVACGPGTHFGGELGQCVSCMPGTYQDMEGQLSCTPCPSSDGLGLPGARNVSE
                     CGGQCSPGFFSADGFKPCQACPVGTYQPEPGRTGCFPCGGGLLTKHEGTTSFQDCEAK
                     VHCSPGHHYNTTTHRCIRCPVGTYQPEFGQNHCITCPGNTSTDFDGSTNVTHCKNQHC
                     GGELGDYTGYIESPNYPGDYPANAECVWHIAPPPKRRILIVVPEIFLPIEDECGDVLV
                     MRKSASPTSITTYETCQTYERPIAFTSRSRKLWIQFKSNEGNSGKGFQVPYVTYDEDY
                     QQLIEDIVRDGRLYASENHQEILKDKKLIKALFDVLAHPQNYFKYTAQESKEMFPRSF
                     IKLLRSKVSRFLRPYK"
     exon            200..331
                     /gene="SCUBE1"
                     /inference="alignment:Splign:2.1.0"
     exon            332..460
                     /gene="SCUBE1"
                     /inference="alignment:Splign:2.1.0"
     exon            461..595
                     /gene="SCUBE1"
                     /inference="alignment:Splign:2.1.0"
     exon            596..721
                     /gene="SCUBE1"
                     /inference="alignment:Splign:2.1.0"
     exon            722..838
                     /gene="SCUBE1"
                     /inference="alignment:Splign:2.1.0"
     exon            839..955
                     /gene="SCUBE1"
                     /inference="alignment:Splign:2.1.0"
     exon            956..1078
                     /gene="SCUBE1"
                     /inference="alignment:Splign:2.1.0"
     exon            1079..1195
                     /gene="SCUBE1"
                     /inference="alignment:Splign:2.1.0"
     exon            1196..1318
                     /gene="SCUBE1"
                     /inference="alignment:Splign:2.1.0"
     exon            1319..1438
                     /gene="SCUBE1"
                     /inference="alignment:Splign:2.1.0"
     exon            1439..1543
                     /gene="SCUBE1"
                     /inference="alignment:Splign:2.1.0"
     exon            1544..1660
                     /gene="SCUBE1"
                     /inference="alignment:Splign:2.1.0"
     exon            1661..1798
                     /gene="SCUBE1"
                     /inference="alignment:Splign:2.1.0"
     exon            1799..2002
                     /gene="SCUBE1"
                     /inference="alignment:Splign:2.1.0"
     exon            2003..2164
                     /gene="SCUBE1"
                     /inference="alignment:Splign:2.1.0"
     exon            2165..2332
                     /gene="SCUBE1"
                     /inference="alignment:Splign:2.1.0"
     exon            2333..2494
                     /gene="SCUBE1"
                     /inference="alignment:Splign:2.1.0"
     exon            2495..2692
                     /gene="SCUBE1"
                     /inference="alignment:Splign:2.1.0"
     exon            2693..2845
                     /gene="SCUBE1"
                     /inference="alignment:Splign:2.1.0"
     exon            2846..2925
                     /gene="SCUBE1"
                     /inference="alignment:Splign:2.1.0"
     exon            2926..9795
                     /gene="SCUBE1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3832..3837
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SCUBE1"
                     /note="hexamer: ATTAAA"
     polyA_site      3852
                     /gene="SCUBE1"
                     /note="major polyA site"
     regulatory      9773..9778
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SCUBE1"
                     /note="hexamer: AATAAA"
     polyA_site      9795
                     /gene="SCUBE1"
                     /note="major polyA site"
ORIGIN      
        1 agcccgagcg gtggcggagc ggcgagcagc gagcagcgcc tgcgggagcg gccggtcggt
       61 cgggtccccg cgccccgcac gcccgcacgc ccagcggggc ccgcattgag catgggcgcg
      121 gcggccgtgc gctggcactt gtgcgtgctg ctggccctgg gcacacgcgg gcggctggcc
      181 gggggcagcg ggctcccagg gtcagtcgac gtggatgagt gctcagaggg cacagatgac
      241 tgccacatcg atgccatctg tcagaacacg cccaagtcct acaaatgcct ctgcaagcca
      301 ggctacaagg gggaaggcaa gcagtgtgaa gacattgacg agtgtgagaa tgactactac
      361 aatgggggct gtgtccacga gtgcatcaac atcccgggga actacaggtg tacctgcttt
      421 gatggcttca tgctggcaca cgatggacac aactgcctgg atgtggacga gtgtcaggac
      481 aataatggtg gctgccagca gatctgcgtc aatgccatgg gcagctacga gtgtcagtgc
      541 cacagtggct tcttccttag tgacaaccag catacctgca tccaccgctc caatgagggt
      601 atgaactgca tgaacaaaga ccatggctgt gcccacatct gccgggagac gcccaaaggt
      661 ggggtggcct gcgactgcag gcccggcttt gaccttgccc aaaaccagaa ggactgcaca
      721 ctaacctgta attatggaaa cggaggctgc cagcacagct gtgaggacac agacacaggc
      781 cccacgtgtg gttgccacca gaagtacgcc ctccactcag acggtcgcac gtgcatcgag
      841 acgtgcgcag tcaataacgg aggctgcgac cggacatgca aggacacagc cactggcgtg
      901 cgatgcagct gccccgttgg attcacactg cagccggacg ggaagacatg caaagacatc
      961 aacgagtgcc tggtcaacaa cggaggctgc gaccacttct gccgcaacac cgtgggcagc
     1021 ttcgagtgcg gctgccggaa gggctacaag ctgctcaccg acgagcgcac ctgccaggac
     1081 atcgacgagt gctccttcga gcggacctgt gaccacatct gcatcaactc cccgggcagc
     1141 ttccagtgcc tgtgtcaccg cggctacatc ctctacggga caacccactg cggagatgtg
     1201 gacgagtgca gcatgagcaa cgggagctgt gaccagggct gcgtcaacac caagggcagc
     1261 tacgagtgcg tctgtccccc ggggaggcgg ctccactgga acgggaagga ttgcgtggag
     1321 acaggcaagt gtctttctcg cgccaagacc tccccccggg cccagctgtc ctgcagcaag
     1381 gcaggcggtg tggagagctg cttcctttcc tgcccggctc acacactctt cgtgccagac
     1441 tcggaaaata gctacgtcct gagctgcgga gttccagggc cgcagggcaa ggcgctgcag
     1501 aaacgcaacg gcaccagctc tggcctcggg cccagctgct cagatgcccc caccaccccc
     1561 atcaaacaga aggcccgctt caagatccga gatgccaagt gccacctccg gccccacagc
     1621 caggcacgag caaaggagac cgccaggcag ccgctgctgg accactgcca tgtgactttc
     1681 gtgaccctca agtgtgactc ctccaagaag aggcgccgtg gccgcaagtc cccatccaag
     1741 gaggtgtccc acatcacagc agagtttgag atcgagacaa agatggaaga ggcctcagac
     1801 acatgcgaag cggactgctt gcggaagcga gcagaacaga gcctgcaggc cgccatcaag
     1861 accctgcgca agtccatcgg ccggcagcag ttctatgtcc aggtctcagg cactgagtac
     1921 gaggtagccc agaggccagc caaggcgctg gaggggcagg gggcatgtgg cgcaggccag
     1981 gtgctacagg acagcaaatg cgttgcctgt gggcctggca cccacttcgg tggtgagctc
     2041 ggccagtgtg tgtcatgtat gccaggaaca taccaggaca tggaaggcca gctcagttgc
     2101 acaccgtgcc ccagcagcga cgggcttggt ctgcctggtg cccgcaacgt gtcggaatgt
     2161 ggaggccagt gttctccagg cttcttctcg gccgatggct tcaagccctg ccaggcctgc
     2221 cccgtgggca cgtaccagcc tgagcccggg cgcaccggct gcttcccctg tggagggggt
     2281 ttgctcacca aacacgaagg caccacctcc ttccaggact gcgaggctaa agtgcactgc
     2341 tcccccggcc accactacaa caccaccacc caccgctgca tccgctgccc cgtcggcacc
     2401 taccagcccg agtttggcca gaaccactgc atcacctgtc cgggcaacac cagcacagac
     2461 ttcgatggct ccaccaacgt cacacactgc aaaaaccagc actgcggcgg cgagcttggt
     2521 gactacaccg gctacatcga gtcccccaac taccctggcg actacccagc caacgctgaa
     2581 tgcgtctggc acatcgcgcc tcccccaaag cgcaggatcc tcatcgtggt ccctgagatc
     2641 ttcctgccca tcgaggatga gtgcggcgat gttctggtca tgaggaagag tgcctctccc
     2701 acgtccatca ccacctatga gacctgccag acctacgaga ggcccatcgc cttcacctcc
     2761 cgctcccgca agctctggat ccagttcaaa tccaatgaag gcaacagcgg caaaggcttc
     2821 caagtgccct atgtcaccta cgatgaggac taccagcaac tcatagagga catcgtgcgc
     2881 gatgggcgcc tgtacgcctc ggagaaccac caggaaattt tgaaagacaa gaagctgatc
     2941 aaggccctct tcgacgtgct ggcgcatccc cagaactact tcaagtacac agcccaggaa
     3001 tccaaggaga tgttcccacg gtccttcatc aaactgctgc gctccaaagt gtctcggttc
     3061 ctgcggccct acaaataacc ggggggagcg gccctgcctg ggggtggcct ggtccgcgga
     3121 gggtgcacct gccctccaca gtgggagctg catgggcctc cacaccacct tgggaacccc
     3181 atggcactgc ccttcaggga agccgaccag cccatggaga ccgagcccag gcacccttcg
     3241 gacccgctgc ccctgtggga gcaccctgct tcaggaagcc tccctccctc cctctgcctc
     3301 ccttccccag gacaccaaga gcgccctctc ctgagccctg gcagaccgac tgcaggtagc
     3361 aggattgcag gaccctctgc ctggcctggc gtttcaggag agaggggaag tggggcctgt
     3421 gctctgggag gcgtggtcat ccgagacagg agtccagggg agagaggagg ggacaaaggc
     3481 gccgtctggg ggaggtcgat gagcctgtgc tggcatccgc gggccccacg ctttgccaac
     3541 tcctccagcc acaggcaagg ccacggctcc gggctgttgc gctctaaggg ttctgtgatt
     3601 ggatggaaca gagctgctgg ggaggagact ggaagtttct gcattccttc aacagaacat
     3661 ttaatgaagt actctatata tatatataaa tatatatata aatatatata tatacttcta
     3721 tttgtgggta ctttaggaaa atgccctttg gtcactgtaa atatgaattg tgaccccatc
     3781 ccttcccgca tgagcccagt gagtcccagc agctatcagc ctccctgaac gattaaacag
     3841 ctcctcccag catttgcatt tgcccttctg tttctgtgag gccggcagcc cttggggctg
     3901 gggagagcac agcttgcctt ggacttgctt ctgaacacga attcttcgag aactaggctc
     3961 agatgctctg ctggagtgtt ggcctcaccc cagtcccctt gccagcaaca tcactgccct
     4021 tggacgagac tcagtggaag acacttcccc tcggacccgt tcccaggagg tgcaggggcc
     4081 cagcaataaa cggcgaaagg ggccttggct cttgccctgt ggcagtgacc ttggggtccg
     4141 tactatgccc aggtgtcagc aggaaacaag gccttgctca caggagcaca ggctgtacca
     4201 ccacaaggag cctcagagcg gccagtgagg aaaggggaga gagcagagct tgctgtcaga
     4261 ggccaggctc ccagtgcagc ctcctcttgc tacgccatgc cacccccatt cacagggaaa
     4321 cctctttcct tcctataaag gaaagaggtt gaattgactc acatttccac atggcttggg
     4381 aggcctcagg aaacttacaa tcatggcgga gggtgaaggg gaagcaagcg ccttcttcac
     4441 aaggcagcag gagagagaag caagcaggga aatgtcggga gaaagagagt ctgggctgtg
     4501 accgagtgca tgtgtcccac ccagccggaa ggccagcgcc tgggatggag ccagcagaac
     4561 cccatgccga ggtccaggag gccttgcctg ctcttcatct gcctgcgagc tgtaggacct
     4621 gcatgggaaa tggtctgtgt gtttcaggtt aacaaatact gaaaatccca aggaacctgg
     4681 gagaatctga aaatgaactc cagtcttcag ctcgaagttg cctctagggt tcctcctggt
     4741 cacccccggg cccagcctgg ctggcgagaa acaaacacgg tttgagaggc tgtggcacac
     4801 tgaggaccac acagatggag gcagccaagt gaggtccctg gaggttcagg gcttggtgac
     4861 gaggtaggcc gtgatcacat attctacagt ccaaaagagg gcccggagct cagcaattac
     4921 tcacgccgtc acatgtgccc acagtagcgg gctccgcggc gtggctcctg ctgccatcag
     4981 ctgccttctc tgctgaagtc caactcaagt cggagagaga gtggagcctc tgcacttggg
     5041 gagtaggtca gtgtcaacta acaagccctg ccaggggcca ctgccgctcc tctggtcaca
     5101 gtccacgcag gccagccctg gtttctggaa agaggcctcc ctccctccct ttgtggagcc
     5161 gtcagtcatc cccaagatga gaggcgagca tcctgccgag tgagcctgca gcaacagctc
     5221 ctgaggaagg tgccgggagg ggtgtcgcct cttcacgagt gttctccaga gaatcagaac
     5281 ccagaggata cagacgtaga tgtgtgaggg gctgcattat gggaactggc tcacagattc
     5341 tggaggccaa caagtcccac gatcggctgt ctgcgattgg gagaccagga gaagccacac
     5401 cgtaactcag tctgagtcca aagcccgaga agccaggggc caccctgtga gtcccagcat
     5461 ccaaagcccc gagaacgagg aagatggatg tcccagctcc caaagggagg attctccctt
     5521 cctcctcctt cttttctatt ggggccctca acagattgga tggagtggat gtcttcactc
     5581 agtctgccaa ttcccatcct aacctcttct ggaaacaccc tagcatgttt tcccagctag
     5641 ctgggtatcc gttcacccaa ccaagacgac catgcaatca accatcacac ttaaatgttt
     5701 ttgttttgtt ttttttgttt tgttttttgt tttctttttt tgttttcttt tttttttttt
     5761 tttttttttt tgagatggag tctcgctctg tcacccaggc tggagtgcag tggcgcgatc
     5821 ttggttcact gcaagctccg cctcccgggt tcacgccatt ctcctgcctc agcctcccga
     5881 gtagctggga ctacaggcac ccaccacctt gcccggctaa tttttttgta tttttagtag
     5941 agacagggtt tcaccatatt agccaggatg gtctcgatct cctgacctcg tgatccgccc
     6001 acctcggcct cccaaagtgc tgggattaca ggcgtgagcc accacgccca gccagggttt
     6061 tcttttttaa tcctttccag aaacagaaag agctcttaac ctctctgagc tctttctgca
     6121 attcctttgg gcgatttctg aaagttgcca cccacgtact cacagatcca ctgcccgcct
     6181 ctcctcccct catgttccag gactcaccac cgtggtcaga ccatgcccag tgtgcccagg
     6241 gcaaagttgg atgggcaggt gcagacgagg cccagccttc atcctgcttt ccctcgcaag
     6301 cccttctata gctcccagcc cacgggagag gccatgagga gcgcgaggtc gccagcagcc
     6361 ccactgggcc tcccacacca tctgagtgac ttgaattcca gggttgggct ctgtcttgag
     6421 tctccacatc agtctcacac cagggaactt gggggtcccc cgttaaacta cagctgaccc
     6481 tgggcctcag ggcgggatgg tcccaaggtg agctccgtgg acagcaaagc gcatttcttc
     6541 cagttggcat gttgtcgaag ccaatggcag cttgactgat gtgaggtggc tattccacct
     6601 ggttcaagat ggaggtgtag agaaaaggga agtggggaac aaggactggg ccaggtagga
     6661 cggatccacg taacgaggcc caagaaaaaa tggaagctgg gggcagagag tccaggaggc
     6721 ccccatgctg tctgagggga ggccggcata gcccctcctc gactgtcgcc tgagcagctg
     6781 atgaggtccc cagcgccgca cgctgtgctc cagggagagg agccaagaag caagcgggct
     6841 gctgggagtc cctgcactgc catctggccg ggacggaggc gcctgcccgg tctccaggct
     6901 cctctgagct tgggtcagct cctaatggag catggcaccc cagggaacca gaggaactct
     6961 gtgaagaaag gcatggaaaa ccggggtgtc tcccgcacag cttcccagga gtcaggtggg
     7021 acccaggcgt ggggggacct ttctgcccca cattcctggc attcccccca agtttatttc
     7081 tactttccag aaacaagcca cgggaaggga aggaacctgc agcagggttt cgaagacagc
     7141 caggccctcc gtgattgagg gctcatatag ccgctgaggg cagcctgcag ccaggtggcc
     7201 tcacctgctg cccagaatta ggctgtgcct tttcccagtc cttccccatg ccagagcctc
     7261 tccccatctt tccccgcgcc agagcctcac agccacagcc acaccaacct ccctcctctc
     7321 aagcagcaga atcagcctac gctccccagc ctcagtgccc ctgccccaca caggtcacgc
     7381 tggcaaggcg atgaccagtt tccgaaacgc tggatcccca agggtacagt gtggcccagt
     7441 cctcccccac ctccatggtg gcccaggctg cagggctcac ggtgggccca cggtggaggc
     7501 ctcaggcagc tttctctcca gctacccatt ggcttaggac acacagataa agtagagctg
     7561 aaacacgggc caaaacatcc ccagacaagg atggcacaag aggccaaatt tgaaggcctt
     7621 gggagagaag ccatttcccc tacctctaac gagcgtagtt ttctgtctga atggcccccc
     7681 ttttcatgca cgctcgttct taagagacgc accatccgca ggcagcgtct cagtgaagcc
     7741 tgtcccctgg gcttgtccac ggcaggaggc ccctggcctg tttaagccat accgatgctt
     7801 aatttacatt tttggagttg aatattattc cggtgcctcc accaccccca gcagaaaaaa
     7861 taagatactg aaataagttg agtcatttcg caaacattta ctgagcacct cctgtgtgcc
     7921 aggctcctag aaagacaggg agttggtgcc ctgcacagat gacaaactgc cccagacagc
     7981 agacagacaa acaaactgcc ccggacagca gacagacaaa cagacacatt gccctggaca
     8041 gcagagagac aaacagacac actgccccgg acagcagaca gacagacacg ttgccccgga
     8101 cagcagacag aaaaactgcc ccggacagca gacagacaga caaactgccc cagacagcag
     8161 acagacaaac tgccccggaa agcagacagc aaactgcccc agacagcaga cagacagaca
     8221 cacttcccca gacagcagat agacagacac cctgccccag acagcagaca agctgccctg
     8281 gacagcctgc tcaccttgcc tgccacagtg catgccccac agggagaagc cttccttctc
     8341 acccagggag tccagccatt ttcacctccc ctggtccatg ctcaggaagt ctggtcaccc
     8401 tggcaaactg cacctgacat tgaggtccct tttcaccaaa gatgcacaag gtgtccatgc
     8461 cgagccgaca tcattcaggc caagggcaga ggatgtaatg aggggtggag tgaggatgtg
     8521 gagagagagc agagagcgcc tcctattcta ccgcggctgc ctctgccccc acagcaccca
     8581 cagatccctg gcccttccag gagtaagtcc tgggcaggca gagctggggt tggcctgatc
     8641 ccacctgcct tgctcttgga aaggacatca ggtgtgggca aagcatcctt gaagcccaga
     8701 caacacaggc atggcctgcg tgccctccca gcatctgctg cgatggcctc aaggcctctc
     8761 cagtcccctg ctgcaccatg ccactcagag cccctgagtg agccccctgg aattgtctgc
     8821 accccccaag ccccaacagg aagaagggaa aggtggggaa gctgacgctc actcagtgcc
     8881 cactgggtgc caggctctgg gctcaaccct ctaatatcca cacattccat ccccccagcc
     8941 ccatgtagat ggtggggtta agaccattct tcagatgtgg aaatgaaggc tctgggagac
     9001 cacaggacat gcccaaggca aagagttggt agtccctgga tttgggaatc aaactcaagc
     9061 ccatctaatc caagggtagg aaatgtctct aaaacgttga tcaatctgaa gcttacagat
     9121 tggaaactgg gccaggtcag ccctggcggt ggagtccaga gacagagaga aggctgcagg
     9181 gaggggagag aacaggagga tcccgggcca gggtgacttc agtgacattc cttccctcct
     9241 tcacaagcac gaaaaactcg ttcggagaaa agacagcttc tgccttccct accaggcgga
     9301 cagggtactc actgtgccga cgtgagcaga cacagccaga ggctcaagtg cccttgccga
     9361 gtgtccccac tgctcttggg gaggccccgg cacagccctg cagagatgca agtaagagag
     9421 tgcccagtgc cctcggcaga tgggtggcag aaagcaggag gaggaagagg gagctgaccg
     9481 atgccgggtg tcttactccg ctgagatcag aacctccaac atgccgagca gacgcaggcc
     9541 acattgctcg gggaacagga caccagggcg ctgtgctagc ggcccgcaga cttcacttct
     9601 ggctcctgtg agggcactgt attgtccaga cgcttggcaa cgactctgcc tgcaagtggc
     9661 aaataccaac ataaccacat cttcctttta gttgccacaa atattcagtt ttgttttatc
     9721 catcatgtcc cccatatgca tgtaatgaaa catttgtgaa aatcacttta ataataaaca
     9781 ggaaatgaac ctaaa
//
