LOCUS       NM_032547               5114 bp    mRNA    linear   PRI 12-FEB-2025
DEFINITION  Homo sapiens short coiled-coil protein (SCOC), transcript variant
            4, mRNA.
ACCESSION   NM_032547
VERSION     NM_032547.3
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Wirth,M., Mouilleron,S., Zhang,W., Sjottem,E., Princely Abudu,Y.,
            Jain,A., Lauritz Olsvik,H., Bruun,J.A., Razi,M., Jefferies,H.B.J.,
            Lee,R., Joshi,D., O'Reilly,N., Johansen,T. and Tooze,S.A.
  TITLE     Phosphorylation of the LIR Domain of SCOC Modulates ATG8 Binding
            Affinity and Specificity
  JOURNAL   J Mol Biol 433 (13), 166987 (2021)
   PUBMED   33845085
  REMARK    GeneRIF: Phosphorylation of the LIR Domain of SCOC Modulates ATG8
            Binding Affinity and Specificity.
            Erratum:[J Mol Biol. 2021 Jul 23;433(15):167049. doi:
            10.1016/j.jmb.2021.167049.. PMID: 34052024]
REFERENCE   2  (bases 1 to 5114)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 5114)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 5114)
  AUTHORS   Stringer,S., Minica,C.C., Verweij,K.J., Mbarek,H., Bernard,M.,
            Derringer,J., van Eijk,K.R., Isen,J.D., Loukola,A.,
            Maciejewski,D.F., Mihailov,E., van der Most,P.J., Sanchez-Mora,C.,
            Roos,L., Sherva,R., Walters,R., Ware,J.J., Abdellaoui,A.,
            Bigdeli,T.B., Branje,S.J., Brown,S.A., Bruinenberg,M., Casas,M.,
            Esko,T., Garcia-Martinez,I., Gordon,S.D., Harris,J.M.,
            Hartman,C.A., Henders,A.K., Heath,A.C., Hickie,I.B., Hickman,M.,
            Hopfer,C.J., Hottenga,J.J., Huizink,A.C., Irons,D.E., Kahn,R.S.,
            Korhonen,T., Kranzler,H.R., Krauter,K., van Lier,P.A., Lubke,G.H.,
            Madden,P.A., Magi,R., McGue,M.K., Medland,S.E., Meeus,W.H.,
            Miller,M.B., Montgomery,G.W., Nivard,M.G., Nolte,I.M.,
            Oldehinkel,A.J., Pausova,Z., Qaiser,B., Quaye,L.,
            Ramos-Quiroga,J.A., Richarte,V., Rose,R.J., Shin,J.,
            Stallings,M.C., Stiby,A.I., Wall,T.L., Wright,M.J., Koot,H.M.,
            Paus,T., Hewitt,J.K., Ribases,M., Kaprio,J., Boks,M.P., Snieder,H.,
            Spector,T., Munafo,M.R., Metspalu,A., Gelernter,J., Boomsma,D.I.,
            Iacono,W.G., Martin,N.G., Gillespie,N.A., Derks,E.M. and Vink,J.M.
  TITLE     Genome-wide association study of lifetime cannabis use based on a
            large meta-analytic sample of 32 330 subjects from the
            International Cannabis Consortium
  JOURNAL   Transl Psychiatry 6 (3), e769 (2016)
   PUBMED   27023175
  REMARK    GeneRIF: SCOC SNPs were associated with lifetime cannabis use, but
            the association did not reach genome-wide significance.
            [Meta-Analysis]
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5114)
  AUTHORS   Alborghetti,M.R., Furlan Ada,S., da Silva,J.C., Sforca,M.L.,
            Honorato,R.V., Granato,D.C., dos Santos Migueleti,D.L., Neves,J.L.,
            de Oliveira,P.S., Paes-Leme,A.F., Zeri,A.C., de Torriani,I.C. and
            Kobarg,J.
  TITLE     Structural analysis of intermolecular interactions in the kinesin
            adaptor complex fasciculation and elongation protein zeta 1/ short
            coiled-coil protein (FEZ1/SCOCO)
  JOURNAL   PLoS One 8 (10), e76602 (2013)
   PUBMED   24116125
  REMARK    GeneRIF: Studies indicate that FEZ1 (fasciculation and elongation
            protein zeta 1), SCOCO (short coiled-coil protein) and kinesins
            (kinesin heavy chain) are involved in biological transport process.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5114)
  AUTHORS   Behrens,C., Binotti,B., Schmidt,C., Robinson,C.V., Chua,J.J. and
            Kuhnel,K.
  TITLE     Crystal structure of the human short coiled coil protein and
            insights into SCOC-FEZ1 complex formation
  JOURNAL   PLoS One 8 (10), e76355 (2013)
   PUBMED   24098481
  REMARK    GeneRIF: SCOC forms a stable homogeneous complex with the coiled
            coil domain of FEZ1. SCOC dimerization and the SCOC surface residue
            R117 are important for this interaction.
            Erratum:[PLoS One. 2013;8(10).
            doi:10.1371/annotation/cb60d973-58aa-45e0-bbb3-1c7f5a42a8d2]
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 5114)
  AUTHORS   McKnight,N.C., Jefferies,H.B., Alemu,E.A., Saunders,R.E.,
            Howell,M., Johansen,T. and Tooze,S.A.
  TITLE     Genome-wide siRNA screen reveals amino acid starvation-induced
            autophagy requires SCOC and WAC
  JOURNAL   EMBO J 31 (8), 1931-1946 (2012)
   PUBMED   22354037
  REMARK    GeneRIF: The identification of SCOC and WAC as novel regulatory
            proteins with diverse functions in autophagy contributes towards a
            fuller understanding of autophagosome formation.
REFERENCE   8  (bases 1 to 5114)
  AUTHORS   Miyamoto-Sato,E., Fujimori,S., Ishizaka,M., Hirai,N., Masuoka,K.,
            Saito,R., Ozawa,Y., Hino,K., Washio,T., Tomita,M., Yamashita,T.,
            Oshikubo,T., Akasaka,H., Sugiyama,J., Matsumoto,Y. and Yanagawa,H.
  TITLE     A comprehensive resource of interacting protein regions for
            refining human transcription factor networks
  JOURNAL   PLoS One 5 (2), e9289 (2010)
   PUBMED   20195357
  REMARK    Publication Status: Online-Only
REFERENCE   9  (bases 1 to 5114)
  AUTHORS   Van Valkenburgh,H., Shern,J.F., Sharer,J.D., Zhu,X. and Kahn,R.A.
  TITLE     ADP-ribosylation factors (ARFs) and ARF-like 1 (ARL1) have both
            specific and shared effectors: characterizing ARL1-binding proteins
  JOURNAL   J Biol Chem 276 (25), 22826-22837 (2001)
   PUBMED   11303027
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK027797.1, AC114771.3,
            AC093671.3 and AA213765.1.
            
            On Jun 2, 2019 this sequence version replaced NM_032547.2.
            
            Summary: This gene encodes a short coiled-coiled domain-containing
            protein that localizes to the Golgi apparatus. The encoded protein
            interacts with ADP-ribosylation factor-like proteins. Pseudogenes
            of this gene are found on chromosomes 1 and 14. Alternative
            splicing results in multiple transcript variants. [provided by
            RefSeq, Apr 2009].
            
            Transcript Variant: This variant (4) differs in the 5' UTR and 5'
            coding region, compared to variant 1, resulting in an isoform (4)
            with a distinct and shorter N-terminus, compared to isoform 1.
            Variants 4, 5 and 6 encode the same isoform.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK027797.1, DA830016.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-160               AK027797.1         23-182
            161-161             AC114771.3         92736-92736
            162-528             AK027797.1         184-550
            529-590             AC093671.3         10255-10316
            591-1936            AK027797.1         613-1958
            1937-1942           AA213765.1         11-16               c
            1943-5114           AC093671.3         11669-14840
FEATURES             Location/Qualifiers
     source          1..5114
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4q31.1"
     gene            1..5114
                     /gene="SCOC"
                     /gene_synonym="HRIHFB2072; SCOCO; UNC-69"
                     /note="short coiled-coil protein"
                     /db_xref="GeneID:60592"
                     /db_xref="HGNC:HGNC:20335"
     exon            1..103
                     /gene="SCOC"
                     /gene_synonym="HRIHFB2072; SCOCO; UNC-69"
                     /inference="alignment:Splign:2.1.0"
     exon            104..191
                     /gene="SCOC"
                     /gene_synonym="HRIHFB2072; SCOCO; UNC-69"
                     /inference="alignment:Splign:2.1.0"
     CDS             122..490
                     /gene="SCOC"
                     /gene_synonym="HRIHFB2072; SCOCO; UNC-69"
                     /note="isoform 4 is encoded by transcript variant 4"
                     /codon_start=1
                     /product="short coiled-coil protein isoform 4"
                     /protein_id="NP_115936.2"
                     /db_xref="CCDS:CCDS3750.1"
                     /db_xref="GeneID:60592"
                     /db_xref="HGNC:HGNC:20335"
                     /translation="MDGSRKEEEEDSTFTNISLADDIDHSSRILYPRPKSLLPKMMNA
                     DMDAVDAENQVELEEKTRLINQVLELQHTLEDLSARVDAVKEENLKLKSENQVLGQYI
                     ENLMSASSVFQTTDTKSKRK"
     exon            192..263
                     /gene="SCOC"
                     /gene_synonym="HRIHFB2072; SCOCO; UNC-69"
                     /inference="alignment:Splign:2.1.0"
     exon            264..347
                     /gene="SCOC"
                     /gene_synonym="HRIHFB2072; SCOCO; UNC-69"
                     /inference="alignment:Splign:2.1.0"
     exon            348..5114
                     /gene="SCOC"
                     /gene_synonym="HRIHFB2072; SCOCO; UNC-69"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1919..1924
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SCOC"
                     /gene_synonym="HRIHFB2072; SCOCO; UNC-69"
                     /note="hexamer: AATAAA"
     polyA_site      1940
                     /gene="SCOC"
                     /gene_synonym="HRIHFB2072; SCOCO; UNC-69"
                     /note="major polyA site"
     regulatory      5090..5095
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SCOC"
                     /gene_synonym="HRIHFB2072; SCOCO; UNC-69"
                     /note="hexamer: AATAAA"
     polyA_site      5114
                     /gene="SCOC"
                     /gene_synonym="HRIHFB2072; SCOCO; UNC-69"
ORIGIN      
        1 atcactacgg agatgctctt gcctatgata ggaacgatgg ctacatctga agctgagcaa
       61 atttatcttt tctcttcgtc tgcatcaggg tgacctctgg aaggtctgaa aattgaacaa
      121 gatggacggg tccaggaaag aggaggagga agacagcaca ttcaccaaca tttctcttgc
      181 agatgacata gaccattcct caagaatttt gtatccaagg cccaaaagtt tgttacccaa
      241 gatgatgaat gctgacatgg atgcagttga tgctgaaaat caagtggaac tggaggaaaa
      301 aacaagactt attaatcaag tgttggaact ccaacacaca cttgaagatc tctctgcaag
      361 agtagatgca gttaaggaag aaaatctgaa gctaaaatca gaaaaccaag ttcttggaca
      421 atatatagaa aatctcatgt cagcttctag tgtttttcaa acaactgaca caaaaagcaa
      481 aagaaagtaa gggattgaca cccttctgtt ttatggaatt gctgctgatc attttttctt
      541 taaaacttgg atagattcca aaagttacag tacctttgtg gcttcattga atatttatga
      601 agataatgtc agatgtagac aaaaataaca caataacagg agacttccat aagtttgtgt
      661 attatgttag tctatgaaaa cgtgcaaatg tattgtagag actttatgat tagaattgca
      721 tatatttatg aaacttaaag atgaatgttt tattgaattt gtaggtttag cactgtcttt
      781 tattatagga ttagtaagat atacaagaaa ataaccaccg tgttgtgaaa aagtgaccaa
      841 aatcatgtac taaatgcaca gctttatgta ccctgtccac catcttgtgc ctcttctcca
      901 tttgcctctt ccttcctatt tcccttccgc taaggaaaaa aattggtgtc acatttgtaa
      961 aagtaatttt aatagttaat catctctgag agtaacctgt attttaattg ttgaaactta
     1021 accaaaataa gatactgtct cagctagggc ttgtcatttg tgtatttagt gttaagatag
     1081 gaatgctagt gtctctttaa ttaattggaa atagatggag gctaaaaatg aaggtttttc
     1141 tttgaaactg aattaacttg ggaatatttg ttgttaaaaa cttctttttg cccaaaataa
     1201 ctcattttgt attatctgaa aatatataat ttctggtcat gtgtatgtta aaatagaaaa
     1261 ttttgaggaa aaatggaaat agggtggaaa agtactcggt aaacagtagt aaccaaatat
     1321 tttcactcca gatttgtgtt ttctctggca cagagtagat cttttgggaa atatatatga
     1381 aagtggatta agtttgacta cccttatgtt agccacatct ggatgagaac agttacaaag
     1441 agtttggtct ctaagttgat ttgtacccag tgggtcaact tctgcaaaat tccgtaatgg
     1501 tgtattagta ttagaatagt gaataaaatg ggaaagttat acatgtatac ttattatctt
     1561 gctcagtatt ttatctcact tgttctagaa ttttctgtaa accctgctac tgggtttgaa
     1621 gagttttagt catcctttaa caatttttaa aaatttagct tctagattcc atttggtaag
     1681 gaaatcaata ttggaagtat tgctaaaatc ttataatatg aaaagagatc cactaatgta
     1741 gcttaaggtt attagatttg ggcttttaat catggaataa tcttatgtat tggtgtaaga
     1801 gttgatgaat gactttagct gtgtgaatat ataatagtca aactgcaaac attttgcatc
     1861 ccttttgtga cctaatttac agacatttaa attgtgttgc agttctgctt tgccgtttaa
     1921 taaaaagcta tttcagaggt tgtgtgtgtt ttgatatagt tgcctaagta aatggggtat
     1981 ttaggtatag tggtgtatcc agggttattc aatgccacag aatagtgtat cttatttaag
     2041 tacccagtta ctgattattt aggggaataa tatttttatt aatacaacta taactggtta
     2101 tcaatataaa tatcataggc tattatacat taatttggaa aacaaaagca aagcactcaa
     2161 aggatccagt atctccaggg aaaaaggaag ttgtattttt tagggtaggc taagctactt
     2221 tagcaagaga cccagtgata aagaatataa atatagaagt ttctctccca accagtggtt
     2281 ccaaggtcag cattgcaggc tagcagccct tgttcaaaca gttattcagg gactttggct
     2341 gaggatggtg ctgccatttt aaatcatggc tttcaagtca ctctagttat tcttctaatt
     2401 tgcagaaaaa gaacatggaa gtgtacatat gcaaggtgtt atgggctgtg cctggaaatg
     2461 gctcatatat cttctcatat tccagtggtg tggagactta gttacatcta gatacaagag
     2521 gagctgagaa atgtagtccc tggctgggaa gccatcttcc agctataaat acaagatttt
     2581 ggtggctgct agcagcctca gccacagctc ttcttgtgct accaagtatc ttcccttaca
     2641 cagaaagtaa tccctcttca tccagctagt gattcagctc acaatctagg atctctatta
     2701 aaggtccatc cctttacatg tggcttctaa tagtctgaca atctctaaac tgataggcaa
     2761 attatttgct ctcacccttg ctgctgccca tttataatgt agttgggaca ggatggccaa
     2821 ataaaaagtc tcaatttgga aaatggaata aagggaaacg taaaaattgt ggaatgctcc
     2881 tgggagatgt atgaagattt tgtccctagc agtggagaag ttccttgatt caactatgat
     2941 tctgatctct ggcaggggac tcccttgttc attgaactcc atggtttccc ctctggaaag
     3001 ttactcctta tccattttct tccatggcta cctcagaagt agagattggg tgttcactcc
     3061 atgggaattg tgaagacagt ccacttcctg ctggtgtgaa tttggtgccc atcattttaa
     3121 ggccaagctc atgtttctgt ggaatacgat tctctcaaaa actcaagcct ttttatcagt
     3181 gtgcttctga ttgcatatgc cgaggaacca caaccaagct tttgaccaaa ttctcaaagt
     3241 atgtattatt tttgcctttc tttaatggag gctatcttga gatcaactaa aaataagatt
     3301 acactgggaa tgttacatgc agatcatcac tgcaaagctg ggttcctttg gcttaacgat
     3361 tggtagagaa gtcttcatgg gcttcccccc aatctccccc acctcaaaaa taattttgaa
     3421 cctttgctgc tattcagact tcagaaacaa ttggcttaat ttcatgaggt tccttgtatc
     3481 aaaactttgt ctattccctt ctatctttgc ttgaaaatgt caattttttt gataccttgc
     3541 tgaaagcaag gaatagccaa ttcacttaac attctggctt tttccagcta gagctatcag
     3601 attagtaggt gtcaaaatat atccccactg cttgtcatgt taatggtaag ctaatgctat
     3661 atacttagtt tttgctatgc aatacttcag gataatttct tcattagaaa tataggtggt
     3721 acacagtgtg cacagggact gtgtccagct tctggaaaag actcgaccaa attttagaca
     3781 ggcacctctg agccctgttt ttgactagtc cagttttagc aaaaatcctg ctaaatgagt
     3841 ttagagataa tcccatcctc aataactgat gatcgtcaac ctggcctgtc tttggcaaga
     3901 atcaccctac tcttgatgtc tccagttagt agttttccat ccactgacct catcactgct
     3961 cattgactat aaatcctcag ttgtatttgc tgtgttagtt gagcccagtt tctttccctt
     4021 attgggtgat ggtcttaaca tatacagtcc tgagtaaagt cttctttact gctttaacaa
     4081 gtgtcagaat ggtttttcct ttaatacttc ctagtttaca tgctggtaga ataatgtttg
     4141 gtagggaaaa atcagcatac tccttgttat ggactgaatg tgtcctccca aaattcttag
     4201 gttgaaatcc taaccccaat gtgatgcttt taggaggtgg ggcttttggg agataattag
     4261 gtaatgaggg tgaaaccctc atgaatggga ttgtgtcatt aaaagaaacc ccagagagct
     4321 ctctttctgc cacatagaag acagcagtct gcatcttgga agagggccct caacactgat
     4381 ctctcagact tccagcttcc aaaactgaga aataaatttg tttataagcc acccagtcta
     4441 tattttgtta tagcatccca aatagtttaa ggcacccatt ttccccactg tttcccaata
     4501 caaatttccc cagtattgac actgccattc tatgcaaaat gtgtcctctt tatagtccct
     4561 ttggactcac agcaggttac tacacatcac agatcctcgt ctgaaacttg tagggccaga
     4621 tgtcttagaa tttggagttt tccagatttt ataaaatatt atgactcaga caccaatatg
     4681 gtttggggca acacctataa tcaagtatat taacatttct gtagcaaaat gagtattaaa
     4741 gtaggataaa gaaagactac atatgtacat aaaaccttgc cggggaagtc aggctcagct
     4801 accaggtgag ttttgttgcc aaaatgctag gaaaatcttt aacttttcag agatttttgg
     4861 tcatggaatt gtagcaacaa aaccttttgt agaagagtaa ccaaaatcat gtatgcattt
     4921 agttccaact agttaatgtc acatctctat tcctgtcacc cttccctgtg atctagtccc
     4981 ttttgtaaga gaaatatggt agttgaatta taaaatctaa aattaatttt gtggtccaaa
     5041 gaaaagcaaa atagatcttt agaatgtaaa gccattcttt actttgccca ataaaaattt
     5101 tgttattccc tgaa
//
