LOCUS       NM_002977               9771 bp    mRNA    linear   PRI 08-NOV-2023
DEFINITION  Homo sapiens sodium voltage-gated channel alpha subunit 9 (SCN9A),
            transcript variant 1, mRNA.
ACCESSION   NM_002977
VERSION     NM_002977.3
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9771)
  AUTHORS   Wisedchaisri G, Gamal El-Din TM, Powell NM, Zheng N and Catterall
            WA.
  TITLE     Structural basis for severe pain caused by mutations in the voltage
            sensors of sodium channel NaV1.7
  JOURNAL   J Gen Physiol 155 (12) (2023)
   PUBMED   37903281
  REMARK    GeneRIF: Structural basis for severe pain caused by mutations in
            the voltage sensors of sodium channel NaV1.7.
REFERENCE   2  (bases 1 to 9771)
  AUTHORS   Yammine T, Aprahamian R, Souaid M, Salem N, Awwad J and Farra C.
  TITLE     Novel SCN9A variant associated with congenital insensitivity to
            pain
  JOURNAL   Mol Biol Rep 50 (7), 6293-6298 (2023)
   PUBMED   37231219
  REMARK    GeneRIF: Novel SCN9A variant associated with congenital
            insensitivity to pain.
REFERENCE   3  (bases 1 to 9771)
  AUTHORS   Jami S, Deuis JR, Klasfauseweh T, Cheng X, Kurdyukov S, Chung F,
            Okorokov AL, Li S, Zhang J, Cristofori-Armstrong B, Israel MR, Ju
            RJ, Robinson SD, Zhao P, Ragnarsson L, Andersson A, Tran P,
            Schendel V, McMahon KL, Tran HNT, Chin YK, Zhu Y, Liu J, Crawford
            T, Purushothamvasan S, Habib AM, Andersson DA, Rash LD, Wood JN,
            Zhao J, Stehbens SJ, Mobli M, Leffler A, Jiang D, Cox JJ, Waxman
            SG, Dib-Hajj SD, Neely GG, Durek T and Vetter I.
  TITLE     Pain-causing stinging nettle toxins target TMEM233 to modulate
            NaV1.7 function
  JOURNAL   Nat Commun 14 (1), 2442 (2023)
   PUBMED   37117223
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 9771)
  AUTHORS   Cox JJ, Reimann F, Nicholas AK, Thornton G, Roberts E, Springell K,
            Karbani G, Jafri H, Mannan J, Raashid Y, Al-Gazali L, Hamamy H,
            Valente EM, Gorman S, Williams R, McHale DP, Wood JN, Gribble FM
            and Woods CG.
  TITLE     An SCN9A channelopathy causes congenital inability to experience
            pain
  JOURNAL   Nature 444 (7121), 894-898 (2006)
   PUBMED   17167479
  REMARK    GeneRIF: data suggest that SCN9A is an essential and non-redundant
            requirement for nociception in humans
            GeneRIF: An SCN9A channelopathy causes congenital inability to
            experience pain
REFERENCE   5  (bases 1 to 9771)
  AUTHORS   Plummer NW and Meisler MH.
  TITLE     Evolution and diversity of mammalian sodium channel genes
  JOURNAL   Genomics 57 (2), 323-331 (1999)
   PUBMED   10198179
  REMARK    Review article
REFERENCE   6  (bases 1 to 9771)
  AUTHORS   Sangameswaran L, Fish LM, Koch BD, Rabert DK, Delgado SG, Ilnicka
            M, Jakeman LB, Novakovic S, Wong K, Sze P, Tzoumaka E, Stewart GR,
            Herman RC, Chan H, Eglen RM and Hunter JC.
  TITLE     A novel tetrodotoxin-sensitive, voltage-gated sodium channel
            expressed in rat and human dorsal root ganglia
  JOURNAL   J Biol Chem 272 (23), 14805-14809 (1997)
   PUBMED   9169448
REFERENCE   7  (bases 1 to 9771)
  AUTHORS   Klugbauer N, Lacinova L, Flockerzi V and Hofmann F.
  TITLE     Structure and functional expression of a new member of the
            tetrodotoxin-sensitive voltage-activated sodium channel family from
            human neuroendocrine cells
  JOURNAL   EMBO J 14 (6), 1084-1090 (1995)
   PUBMED   7720699
REFERENCE   8  (bases 1 to 9771)
  AUTHORS   Schon,K.R., Parker,A.P.J. and Woods,C.G.
  TITLE     Congenital Insensitivity to Pain Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE, Bean LJH,
            Gripp KW and Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   29419974
REFERENCE   9  (bases 1 to 9771)
  AUTHORS   Kurth,I.
  TITLE     Hereditary Sensory and Autonomic Neuropathy Type II
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE, Bean LJH,
            Gripp KW and Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   21089229
REFERENCE   10 (bases 1 to 9771)
  AUTHORS   Hisama,F.M., Dib-Hajj,S.D. and Waxman,S.G.
  TITLE     SCN9A Neuropathic Pain Syndromes
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE, Bean LJH,
            Gripp KW and Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301342
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL517958.3, AK307549.1,
            AC108146.3, DQ857292.1, X82835.1, AC107082.3 and BU742924.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            [WARNING] On Dec 15, 2023 this sequence was replaced by
            NM_002977.4.
            
            On Aug 19, 2009 this sequence version replaced NM_002977.2.
            
            Summary: This gene encodes a voltage-gated sodium channel which
            plays a significant role in nociception signaling. Mutations in
            this gene have been associated with primary erythermalgia,
            channelopathy-associated insensitivity to pain, and paroxysmal
            extreme pain disorder. [provided by RefSeq, Aug 2009].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            CDS exon combination :: X82835.1, DQ857292.1 [ECO:0000331]
            RNAseq introns       :: mixed sample support SAMEA1965299,
                                    SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-27                AL517958.3         6-32
            28-514              AK307549.1         1-487
            515-515             AC108146.3         67236-67236         c
            516-784             DQ857292.1         216-484
            785-785             AC108146.3         62186-62186         c
            786-6522            X82835.1           493-6229
            6523-6650           X82835.1           6234-6361
            6651-9186           AC107082.3         62358-64893         c
            9187-9771           BU742924.1         1-585               c
FEATURES             Location/Qualifiers
     source          1..9771
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q24.3"
     gene            1..9771
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; NE-NA;
                     NENA; PN1; SFNP"
                     /note="sodium voltage-gated channel alpha subunit 9"
                     /db_xref="GeneID:6335"
                     /db_xref="HGNC:HGNC:10597"
                     /db_xref="MIM:603415"
     exon            1..291
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; NE-NA;
                     NENA; PN1; SFNP"
                     /inference="alignment:Splign:2.1.0"
     exon            292..599
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; NE-NA;
                     NENA; PN1; SFNP"
                     /inference="alignment:Splign:2.1.0"
     CDS             342..6275
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; NE-NA;
                     NENA; PN1; SFNP"
                     /note="isoform 1 is encoded by transcript variant 1;
                     sodium channel, voltage-gated, type IX, alpha polypeptide;
                     sodium channel protein type 9 subunit alpha; sodium
                     channel, voltage-gated, type IX, alpha subunit;
                     voltage-gated sodium channel alpha subunit Nav1.7; hNE-Na;
                     peripheral sodium channel 1; neuroendocrine sodium
                     channel; sodium channel protein type IX subunit alpha;
                     voltage-gated sodium channel subunit alpha Nav1.7"
                     /codon_start=1
                     /product="sodium channel protein type 9 subunit alpha
                     isoform 1"
                     /protein_id="NP_002968.1"
                     /db_xref="CCDS:CCDS46441.1"
                     /db_xref="GeneID:6335"
                     /db_xref="HGNC:HGNC:10597"
                     /db_xref="MIM:603415"
                     /translation="MAMLPPPGPQSFVHFTKQSLALIEQRIAERKSKEPKEEKKDDDE
                     EAPKPSSDLEAGKQLPFIYGDIPPGMVSEPLEDLDPYYADKKTFIVLNKGKTIFRFNA
                     TPALYMLSPFSPLRRISIKILVHSLFSMLIMCTILTNCIFMTMNNPPDWTKNVEYTFT
                     GIYTFESLVKILARGFCVGEFTFLRDPWNWLDFVVIVFAYLTEFVNLGNVSALRTFRV
                     LRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLKHKCF
                     RNSLENNETLESIMNTLESEEDFRKYFYYLEGSKDALLCGFSTDSGQCPEGYTCVKIG
                     RNPDYGYTSFDTFSWAFLALFRLMTQDYWENLYQQTLRAAGKTYMIFFVVVIFLGSFY
                     LINLILAVVAMAYEEQNQANIEEAKQKELEFQQMLDRLKKEQEEAEAIAAAAAEYTSI
                     RRSRIMGLSESSSETSKLSSKSAKERRNRRKKKNQKKLSSGEEKGDAEKLSKSESEDS
                     IRRKSFHLGVEGHRRAHEKRLSTPNQSPLSIRGSLFSARRSSRTSLFSFKGRGRDIGS
                     ETEFADDEHSIFGDNESRRGSLFVPHRPQERRSSNISQASRSPPMLPVNGKMHSAVDC
                     NGVVSLVDGRSALMLPNGQLLPEGTTNQIHKKRRCSSYLLSEDMLNDPNLRQRAMSRA
                     SILTNTVEELEESRQKCPPWWYRFAHKFLIWNCSPYWIKFKKCIYFIVMDPFVDLAIT
                     ICIVLNTLFMAMEHHPMTEEFKNVLAIGNLVFTGIFAAEMVLKLIAMDPYEYFQVGWN
                     IFDSLIVTLSLVELFLADVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALG
                     NLTLVLAIIVFIFAVVGMQLFGKSYKECVCKINDDCTLPRWHMNDFFHSFLIVFRVLC
                     GEWIETMWDCMEVAGQAMCLIVYMMVMVIGNLVVLNLFLALLLSSFSSDNLTAIEEDP
                     DANNLQIAVTRIKKGINYVKQTLREFILKAFSKKPKISREIRQAEDLNTKKENYISNH
                     TLAEMSKGHNFLKEKDKISGFGSSVDKHLMEDSDGQSFIHNPSLTVTVPIAPGESDLE
                     NMNAEELSSDSDSEYSKVRLNRSSSSECSTVDNPLPGEGEEAEAEPMNSDEPEACFTD
                     GCVRRFSCCQVNIESGKGKIWWNIRKTCYKIVEHSWFESFIVLMILLSSGALAFEDIY
                     IERKKTIKIILEYADKIFTYIFILEMLLKWIAYGYKTYFTNAWCWLDFLIVDVSLVTL
                     VANTLGYSDLGPIKSLRTLRALRPLRALSRFEGMRVVVNALIGAIPSIMNVLLVCLIF
                     WLIFSIMGVNLFAGKFYECINTTDGSRFPASQVPNRSECFALMNVSQNVRWKNLKVNF
                     DNVGLGYLSLLQVATFKGWTIIMYAAVDSVNVDKQPKYEYSLYMYIYFVVFIIFGSFF
                     TLNLFIGVIIDNFNQQKKKLGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKIQ
                     GCIFDLVTNQAFDISIMVLICLNMVTMMVEKEGQSQHMTEVLYWINVVFIILFTGECV
                     LKLISLRHYYFTVGWNIFDFVVVIISIVGMFLADLIETYFVSPTLFRVIRLARIGRIL
                     RLVKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKKEDGINDMF
                     NFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPKKVHPGSSVEGDCGNPSVGI
                     FYFVSYIIISFLVVVNMYIAVILENFSVATEESTEPLSEDDFEMFYEVWEKFDPDATQ
                     FIEFSKLSDFAAALDPPLLIAKPNKVQLIAMDLPMVSGDRIHCLDILFAFTKRVLGES
                     GEMDSLRSQMEERFMSANPSKVSYEPITTTLKRKQEDVSATVIQRAYRRYRLRQNVKN
                     ISSIYIKDGDRDDDLLNKKDMAFDNVNENSSPEKTDATSSTTSPPSYDSVTKPDKEKY
                     EQDRTEKEDKGKDSKESKK"
     exon            600..718
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; NE-NA;
                     NENA; PN1; SFNP"
                     /inference="alignment:Splign:2.1.0"
     exon            719..808
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; NE-NA;
                     NENA; PN1; SFNP"
                     /inference="alignment:Splign:2.1.0"
     exon            809..937
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; NE-NA;
                     NENA; PN1; SFNP"
                     /inference="alignment:Splign:2.1.0"
     exon            938..1029
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; NE-NA;
                     NENA; PN1; SFNP"
                     /inference="alignment:Splign:2.1.0"
     exon            1030..1242
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; NE-NA;
                     NENA; PN1; SFNP"
                     /inference="alignment:Splign:2.1.0"
     exon            1243..1306
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; NE-NA;
                     NENA; PN1; SFNP"
                     /inference="alignment:Splign:2.1.0"
     exon            1307..1448
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; NE-NA;
                     NENA; PN1; SFNP"
                     /inference="alignment:Splign:2.1.0"
     exon            1449..1655
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; NE-NA;
                     NENA; PN1; SFNP"
                     /inference="alignment:Splign:2.1.0"
     exon            1656..1943
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; NE-NA;
                     NENA; PN1; SFNP"
                     /inference="alignment:Splign:2.1.0"
     exon            1944..2282
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; NE-NA;
                     NENA; PN1; SFNP"
                     /inference="alignment:Splign:2.1.0"
     exon            2283..2412
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; NE-NA;
                     NENA; PN1; SFNP"
                     /inference="alignment:Splign:2.1.0"
     exon            2413..2651
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; NE-NA;
                     NENA; PN1; SFNP"
                     /inference="alignment:Splign:2.1.0"
     exon            2652..2825
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; NE-NA;
                     NENA; PN1; SFNP"
                     /inference="alignment:Splign:2.1.0"
     exon            2826..3182
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; NE-NA;
                     NENA; PN1; SFNP"
                     /inference="alignment:Splign:2.1.0"
     exon            3183..3659
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; NE-NA;
                     NENA; PN1; SFNP"
                     /inference="alignment:Splign:2.1.0"
     exon            3660..3780
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; NE-NA;
                     NENA; PN1; SFNP"
                     /inference="alignment:Splign:2.1.0"
     exon            3781..3935
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; NE-NA;
                     NENA; PN1; SFNP"
                     /inference="alignment:Splign:2.1.0"
     exon            3936..4109
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; NE-NA;
                     NENA; PN1; SFNP"
                     /inference="alignment:Splign:2.1.0"
     exon            4110..4232
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; NE-NA;
                     NENA; PN1; SFNP"
                     /inference="alignment:Splign:2.1.0"
     exon            4233..4514
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; NE-NA;
                     NENA; PN1; SFNP"
                     /inference="alignment:Splign:2.1.0"
     exon            4515..4568
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; NE-NA;
                     NENA; PN1; SFNP"
                     /inference="alignment:Splign:2.1.0"
     exon            4569..4706
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; NE-NA;
                     NENA; PN1; SFNP"
                     /inference="alignment:Splign:2.1.0"
     exon            4707..4811
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; NE-NA;
                     NENA; PN1; SFNP"
                     /inference="alignment:Splign:2.1.0"
     exon            4812..5082
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; NE-NA;
                     NENA; PN1; SFNP"
                     /inference="alignment:Splign:2.1.0"
     exon            5083..9762
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; NE-NA;
                     NENA; PN1; SFNP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6539..6544
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; NE-NA;
                     NENA; PN1; SFNP"
                     /note="hexamer: AATAAA"
     polyA_site      6564
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; NE-NA;
                     NENA; PN1; SFNP"
                     /note="major polyA site"
     regulatory      9740..9745
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; NE-NA;
                     NENA; PN1; SFNP"
                     /note="hexamer: AATAAA"
     polyA_site      9762
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; NE-NA;
                     NENA; PN1; SFNP"
                     /note="major polyA site"
ORIGIN      
        1 cggggctgct acctccacgg gcgcgccctg gcaggagggg cgcagtctgc ttgcaggcgg
       61 tcgccagcgc tccagcggcg gctgtcggct ttccaattcc gccagctcgg ctgaggctgg
      121 gctagcctgg gtgccagtgg ctgctagcgg caggcgtccc ctgagcaaca ggagcccaga
      181 gaaaaagaag cagccctgag agagcgccgg ggaaggagag gcccgcgccc tctcctggag
      241 ccagattctg caggtgcact gggtggggat gatcggcggg ctaggttgca agcctcttat
      301 gtgaggagct gaagaggaat taaaatatac aggatgaaaa gatggcaatg ttgcctcccc
      361 caggacctca gagctttgtc catttcacaa aacagtctct tgccctcatt gaacaacgca
      421 ttgctgaaag aaaatcaaag gaacccaaag aagaaaagaa agatgatgat gaagaagccc
      481 caaagccaag cagtgacttg gaagctggca aacagctgcc cttcatctat ggggacattc
      541 ctcccggcat ggtgtcagag cccctggagg acttggaccc ctactatgca gacaaaaaga
      601 ctttcatagt attgaacaaa gggaaaacaa tcttccgttt caatgccaca cctgctttat
      661 atatgctttc tcctttcagt cctctaagaa gaatatctat taagatttta gtacactcct
      721 tattcagcat gctcatcatg tgcactattc tgacaaactg catatttatg accatgaata
      781 acccaccgga ctggaccaaa aatgtcgagt acacttttac tggaatatat acttttgaat
      841 cacttgtaaa aatccttgca agaggcttct gtgtaggaga attcactttt cttcgtgacc
      901 cgtggaactg gctggatttt gtcgtcattg tttttgcgta tttaacagaa tttgtaaacc
      961 taggcaatgt ttcagctctt cgaactttca gagtattgag agctttgaaa actatttctg
     1021 taatcccagg cctgaagaca attgtagggg ctttgatcca gtcagtgaag aagctttctg
     1081 atgtcatgat cctgactgtg ttctgtctga gtgtgtttgc actaattgga ctacagctgt
     1141 tcatgggaaa cctgaagcat aaatgttttc gaaattcact tgaaaataat gaaacattag
     1201 aaagcataat gaatacccta gagagtgaag aagactttag aaaatatttt tattacttgg
     1261 aaggatccaa agatgctctc ctttgtggtt tcagcacaga ttcaggtcag tgtccagagg
     1321 ggtacacctg tgtgaaaatt ggcagaaacc ctgattatgg ctacacgagc tttgacactt
     1381 tcagctgggc cttcttagcc ttgtttaggc taatgaccca agattactgg gaaaaccttt
     1441 accaacagac gctgcgtgct gctggcaaaa cctacatgat cttctttgtc gtagtgattt
     1501 tcctgggctc cttttatcta ataaacttga tcctggctgt ggttgccatg gcatatgaag
     1561 aacagaacca ggcaaacatt gaagaagcta aacagaaaga attagaattt caacagatgt
     1621 tagaccgtct taaaaaagag caagaagaag ctgaggcaat tgcagcggca gcggctgaat
     1681 atacaagtat taggagaagc agaattatgg gcctctcaga gagttcttct gaaacatcca
     1741 aactgagctc taaaagtgct aaagaaagaa gaaacagaag aaagaaaaag aatcaaaaga
     1801 agctctccag tggagaggaa aagggagatg ctgagaaatt gtcgaaatca gaatcagagg
     1861 acagcatcag aagaaaaagt ttccaccttg gtgtcgaagg gcataggcga gcacatgaaa
     1921 agaggttgtc tacccccaat cagtcaccac tcagcattcg tggctccttg ttttctgcaa
     1981 ggcgaagcag cagaacaagt ctttttagtt tcaaaggcag aggaagagat ataggatctg
     2041 agactgaatt tgccgatgat gagcacagca tttttggaga caatgagagc agaaggggct
     2101 cactgtttgt gccccacaga ccccaggagc gacgcagcag taacatcagc caagccagta
     2161 ggtccccacc aatgctgccg gtgaacggga aaatgcacag tgctgtggac tgcaacggtg
     2221 tggtctccct ggttgatgga cgctcagccc tcatgctccc caatggacag cttctgccag
     2281 agggcacgac caatcaaata cacaagaaaa ggcgttgtag ttcctatctc ctttcagagg
     2341 atatgctgaa tgatcccaac ctcagacaga gagcaatgag tagagcaagc atattaacaa
     2401 acactgtgga agaacttgaa gagtccagac aaaaatgtcc accttggtgg tacagatttg
     2461 cacacaaatt cttgatctgg aattgctctc catattggat aaaattcaaa aagtgtatct
     2521 attttattgt aatggatcct tttgtagatc ttgcaattac catttgcata gttttaaaca
     2581 cattatttat ggctatggaa caccacccaa tgactgagga attcaaaaat gtacttgcta
     2641 taggaaattt ggtctttact ggaatctttg cagctgaaat ggtattaaaa ctgattgcca
     2701 tggatccata tgagtatttc caagtaggct ggaatatttt tgacagcctt attgtgactt
     2761 taagtttagt ggagctcttt ctagcagatg tggaaggatt gtcagttctg cgatcattca
     2821 gactgctccg agtcttcaag ttggcaaaat cctggccaac attgaacatg ctgattaaga
     2881 tcattggtaa ctcagtaggg gctctaggta acctcacctt agtgttggcc atcatcgtct
     2941 tcatttttgc tgtggtcggc atgcagctct ttggtaagag ctacaaagaa tgtgtctgca
     3001 agatcaatga tgactgtacg ctcccacggt ggcacatgaa cgacttcttc cactccttcc
     3061 tgattgtgtt ccgcgtgctg tgtggagagt ggatagagac catgtgggac tgtatggagg
     3121 tcgctggtca agctatgtgc cttattgttt acatgatggt catggtcatt ggaaacctgg
     3181 tggtcctaaa cctatttctg gccttattat tgagctcatt tagttcagac aatcttacag
     3241 caattgaaga agaccctgat gcaaacaacc tccagattgc agtgactaga attaaaaagg
     3301 gaataaatta tgtgaaacaa accttacgtg aatttattct aaaagcattt tccaaaaagc
     3361 caaagatttc cagggagata agacaagcag aagatctgaa tactaagaag gaaaactata
     3421 tttctaacca tacacttgct gaaatgagca aaggtcacaa tttcctcaag gaaaaagata
     3481 aaatcagtgg ttttggaagc agcgtggaca aacacttgat ggaagacagt gatggtcaat
     3541 catttattca caatcccagc ctcacagtga cagtgccaat tgcacctggg gaatccgatt
     3601 tggaaaatat gaatgctgag gaacttagca gtgattcgga tagtgaatac agcaaagtga
     3661 gattaaaccg gtcaagctcc tcagagtgca gcacagttga taaccctttg cctggagaag
     3721 gagaagaagc agaggctgaa cctatgaatt ccgatgagcc agaggcctgt ttcacagatg
     3781 gttgtgtacg gaggttctca tgctgccaag ttaacataga gtcagggaaa ggaaaaatct
     3841 ggtggaacat caggaaaacc tgctacaaga ttgttgaaca cagttggttt gaaagcttca
     3901 ttgtcctcat gatcctgctc agcagtggtg ccctggcttt tgaagatatt tatattgaaa
     3961 ggaaaaagac cattaagatt atcctggagt atgcagacaa gatcttcact tacatcttca
     4021 ttctggaaat gcttctaaaa tggatagcat atggttataa aacatatttc accaatgcct
     4081 ggtgttggct ggatttccta attgttgatg tttctttggt tactttagtg gcaaacactc
     4141 ttggctactc agatcttggc cccattaaat cccttcggac actgagagct ttaagacctc
     4201 taagagcctt atctagattt gaaggaatga gggtcgttgt gaatgcactc ataggagcaa
     4261 ttccttccat catgaatgtg ctacttgtgt gtcttatatt ctggctgata ttcagcatca
     4321 tgggagtaaa tttgtttgct ggcaagttct atgagtgtat taacaccaca gatgggtcac
     4381 ggtttcctgc aagtcaagtt ccaaatcgtt ccgaatgttt tgcccttatg aatgttagtc
     4441 aaaatgtgcg atggaaaaac ctgaaagtga actttgataa tgtcggactt ggttacctat
     4501 ctctgcttca agttgcaact tttaagggat ggacgattat tatgtatgca gcagtggatt
     4561 ctgttaatgt agacaagcag cccaaatatg aatatagcct ctacatgtat atttattttg
     4621 tcgtctttat catctttggg tcattcttca ctttgaactt gttcattggt gtcatcatag
     4681 ataatttcaa ccaacagaaa aagaagcttg gaggtcaaga catctttatg acagaagaac
     4741 agaagaaata ctataatgca atgaaaaagc tggggtccaa gaagccacaa aagccaattc
     4801 ctcgaccagg gaacaaaatc caaggatgta tatttgacct agtgacaaat caagcctttg
     4861 atattagtat catggttctt atctgtctca acatggtaac catgatggta gaaaaggagg
     4921 gtcaaagtca acatatgact gaagttttat attggataaa tgtggttttt ataatccttt
     4981 tcactggaga atgtgtgcta aaactgatct ccctcagaca ctactacttc actgtaggat
     5041 ggaatatttt tgattttgtg gttgtgatta tctccattgt aggtatgttt ctagctgatt
     5101 tgattgaaac gtattttgtg tcccctaccc tgttccgagt gatccgtctt gccaggattg
     5161 gccgaatcct acgtctagtc aaaggagcaa aggggatccg cacgctgctc tttgctttga
     5221 tgatgtccct tcctgcgttg tttaacatcg gcctcctgct cttcctggtc atgttcatct
     5281 acgccatctt tggaatgtcc aactttgcct atgttaaaaa ggaagatgga attaatgaca
     5341 tgttcaattt tgagaccttt ggcaacagta tgatttgcct gttccaaatt acaacctctg
     5401 ctggctggga tggattgcta gcacctattc ttaacagtaa gccacccgac tgtgacccaa
     5461 aaaaagttca tcctggaagt tcagttgaag gagactgtgg taacccatct gttggaatat
     5521 tctactttgt tagttatatc atcatatcct tcctggttgt ggtgaacatg tacattgcag
     5581 tcatactgga gaattttagt gttgccactg aagaaagtac tgaacctctg agtgaggatg
     5641 actttgagat gttctatgag gtttgggaga agtttgatcc cgatgcgacc cagtttatag
     5701 agttctctaa actctctgat tttgcagctg ccctggatcc tcctcttctc atagcaaaac
     5761 ccaacaaagt ccagctcatt gccatggatc tgcccatggt tagtggtgac cggatccatt
     5821 gtcttgacat cttatttgct tttacaaagc gtgttttggg tgagagtggg gagatggatt
     5881 ctcttcgttc acagatggaa gaaaggttca tgtctgcaaa tccttccaaa gtgtcctatg
     5941 aacccatcac aaccacacta aaacggaaac aagaggatgt gtctgctact gtcattcagc
     6001 gtgcttatag acgttaccgc ttaaggcaaa atgtcaaaaa tatatcaagt atatacataa
     6061 aagatggaga cagagatgat gatttactca ataaaaaaga tatggctttt gataatgtta
     6121 atgagaactc aagtccagaa aaaacagatg ccacttcatc caccacctct ccaccttcat
     6181 atgatagtgt aacaaagcca gacaaagaga aatatgaaca agacagaaca gaaaaggaag
     6241 acaaagggaa agacagcaag gaaagcaaaa aatagagctt catttttgat atattgttta
     6301 cagcctgtga aagtgattta tttgtgttaa taaaactctt ttgaggaagt ctatgccaaa
     6361 atccttttta tcaaaatatt ctcgaaggca gtgcagtcac taactctgat ttcctaagaa
     6421 aggtgggcag cattagcaga tggttatttt tgcactgatg attctttaag aatcgtaaga
     6481 gaactctgta ggaattattg attatagcat acaaaagtga ttcagttttt tggtttttaa
     6541 taaatcagaa gaccatgtag aaaactttta catctgcctt gtcatctttt cacaggattg
     6601 taattagtct tgtttcccat gtaaataaac aacacacgca tacagaaaaa tctattattt
     6661 atctattatt tggaaatcaa caaaagtatt tgccttggct ttgcaatgaa atgcttgata
     6721 gaagtaatgg acattagtta tgaatgttta gttaaaatgc attattaggg agcttgactt
     6781 tttatcaatg tacagaggtt attctatatt ttgaggtgct taaatttatt ctacattgca
     6841 tcagaaccaa tttatatgtg cctataaaat gccatgggat taaaaatata tgtaggctat
     6901 tcatttctac aaatgttttt cattcatctt gactcacatg ccaacaagga taagacttac
     6961 ctttagagta ttgtgtttca tagcctttct tctttcatat ccctttttgt tcatagaata
     7021 accacagaac ttgaaaaatt attctaagta catattacac tcctcaaaaa aaacaaagat
     7081 aactgagaaa aaagttattg acagaagttc tatttgctat tatttacata gcctaacatt
     7141 tgactgtgct gcccaaaata ctgataatag tctcttaaac tcttttgtca aattttcctg
     7201 ctttcttatg cagtattgtt tagtcatcct ttcgctgtaa gcaaagttga tgaaatcctt
     7261 cctgatatgc agttagttgt ttgaccacgg tacatacttg agcagataat aacttgggca
     7321 cagtatttat tgcatcactt gtatacaatc ccgtgtttgg caagctttca aatcatgtaa
     7381 tatgacagac tttacacaga tatgtgttta gtatgaataa aaaagcattg aaatagggat
     7441 tcttgccaac ttgctctctt gccaccaact tactttccta aattatggaa gtaatctttt
     7501 ttggatatac ttcaatgtat acaatgagga agatgtcacc ttctccttaa aattctatga
     7561 tgtgaaatat attttgcctc aatcaacaca gtaccatggg cttctaattt atcaagcaca
     7621 tattcatttt gcattagctg tagacatcta gttttttgaa aacacctatt aatagtaatt
     7681 tgaaaagaaa taaccataat gctttttttc gtgagtttat ttcaggaata tgagatcttt
     7741 cttctataaa gttattcatg cacaggcaaa aattgagcta cacaggtaga atgtagtttt
     7801 acttagaaga tttttgtggg aggttttgaa gcaaatatat aaaacaactt tcactaattt
     7861 gctttccata tttaaaaaat aataaattac atttatataa taaatgttta aagcacatat
     7921 tttttgttgt tctggcaatt taaaaagaaa gaggatttaa acgtacctat agaaacaaag
     7981 atttatggtt aaagaatgag atcagaagtc tagaatgttt ttaaattgtg atatatttta
     8041 caacatccgt tattactttg agacatttgt cctaatctac gtataaaact caatctaggg
     8101 ctaaagattc tttataccat cttaggttca ttcatcttag gctatttgaa ccacttttta
     8161 atttaatatg aaagacacca tgcagtgttt tccgagacta catagatcat tttatcacat
     8221 acctaccaag cctgttggaa ataggttttg ataatttaag tagggaccta tacaaaatat
     8281 attacattta tcagattttt aaatacattc aattaagaat ttaacatcac cttaaatttg
     8341 aattcaatct accgttattt caaactcaca aatataactg cattatgaat acttacataa
     8401 tgtagtaaga caagatgttt gacaggttcg tgtgtaattt tctattaatg tttttacatt
     8461 gccttgtttt tatgtaaaat aaaaaatatg ggcaactggt ttgttaacaa cacaatttct
     8521 tcttagcatt tcaaaaatat atataaagtt gttctttttc ctatttcatg aactatgttt
     8581 ttttttaaaa taacatggtt aagttttata tatatttacg tttgtttcag gaatgtctac
     8641 ttgtgacttt ttatcaatta aaaataatat ttggaagaaa gagcttatta agtataagct
     8701 tgaagtaaaa ttagacctct ctttccatgt agattactgt ttgtactgat ggtttcaccc
     8761 ttcagaaggc actgtcatat taatatttaa attttataat cgctgaactt attacaccca
     8821 acaatacaga aaggcagtta cactgaagaa cttaacttag aataaaatgg aagcaaacag
     8881 gttttctaaa aactttttta agtgaccagg tctcgctctg tcacccaggc tagagtgcaa
     8941 tggcatgatc atagctctct gcagcctcaa ctctgggctc aagcaaccct cctgcctcag
     9001 cctcccaagt agctaagact acaggtacat gccaccatgc ctggctaata tttaaatttt
     9061 tgtagataag gggtcttgct atgttgccca ggctagtctc aaactcctgg cttcaagtgt
     9121 tcctactgtc atgacctgcc aacatgctgg ggttacaggc atgagccacc atgccccaaa
     9181 caggtttgaa cacaaatctt tcggatgaaa attagagaac ctaattttag ctttttgata
     9241 gttacctagt ttgcaaaaga tttgggtgac ttgtgagctg tttttaaatg ctgattgttg
     9301 aacatcacaa cccaaaatac ttagcatgat tttatagagt tttgatagct ttattaaaaa
     9361 gagtgaaaat aaaatgcata tgtaaataaa gcagttctaa atagctattt cagagaaatg
     9421 ttaatagaag tgctgaaaga agggccaact aaattaggat ggccagggaa ttggcctggg
     9481 tttaggacct atgtatgaag gccaccaatt ttttaaaaat atctgtggtt tattatgtta
     9541 ttatcttctt gaggaaaaca atcaagaatt gcttcatgaa aataaataaa tagccatgaa
     9601 tatcataaag ctgtttacat aggattcttt acaaatttca tagatctatg aatgctcaaa
     9661 atgtttgagt ttgccataaa ttatattgta gttatattgt agttatactt gagactgaca
     9721 cattgtaata taatctaaga ataaaagtta tacaaaataa aaaaaaaaaa a
//
