LOCUS       NM_001365536            9752 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens sodium voltage-gated channel alpha subunit 9 (SCN9A),
            transcript variant 2, mRNA.
ACCESSION   NM_001365536 XM_005246757
VERSION     NM_001365536.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9752)
  AUTHORS   Kim,J.S., Meeker,S., Ru,F., Tran,M., Zabka,T.S., Hackos,D. and
            Undem,B.J.
  TITLE     Role of NaV1.7 in postganglionic sympathetic nerve function in
            human and guinea-pig arteries
  JOURNAL   J Physiol 602 (14), 3505-3518 (2024)
   PUBMED   38743485
  REMARK    GeneRIF: Role of NaV1.7 in postganglionic sympathetic nerve
            function in human and guinea-pig arteries.
REFERENCE   2  (bases 1 to 9752)
  AUTHORS   Noguchi,A., Tezuka,T., Okuda,H., Kobayashi,H., Harada,K.H.,
            Yoshida,T., Akioka,S., Wada,K., Takeya,A., Kabata-Murasawa,R.,
            Kondo,D., Ishikawa,K., Asano,T., Fujiwara,M., Hishikawa,N.,
            Mizukami,T., Hitomi,T., Youssefian,S., Nagai,Y., Tanaka,M., Eto,K.,
            Shiraishi,H., Amaya,F., Koizumi,A. and Takahashi,T.
  TITLE     Genetic Analysis of SCN11A, SCN10A, and SCN9A in Familial Episodic
            Pain Syndrome (FEPS) in Japan and Proposal of Clinical Diagnostic
            Criteria
  JOURNAL   Int J Mol Sci 25 (13), 6832 (2024)
   PUBMED   38999942
  REMARK    GeneRIF: Genetic Analysis of SCN11A, SCN10A, and SCN9A in Familial
            Episodic Pain Syndrome (FEPS) in Japan and Proposal of Clinical
            Diagnostic Criteria.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 9752)
  AUTHORS   Waheed,S., Ramzan,K., Ahmad,S., Khan,M.S., Wajid,M., Ullah,H.,
            Umar,A., Iqbal,R., Ullah,R. and Bari,A.
  TITLE     Identification and In-Silico study of non-synonymous functional
            SNPs in the human SCN9A gene
  JOURNAL   PLoS One 19 (2), e0297367 (2024)
   PUBMED   38394191
  REMARK    GeneRIF: Identification and In-Silico study of non-synonymous
            functional SNPs in the human SCN9A gene.
            Erratum:[PLoS One. 2024 Mar 26;19(3):e0301489. doi:
            10.1371/journal.pone.0301489.. PMID: 38530842]
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 9752)
  AUTHORS   Cox,J.J., Reimann,F., Nicholas,A.K., Thornton,G., Roberts,E.,
            Springell,K., Karbani,G., Jafri,H., Mannan,J., Raashid,Y.,
            Al-Gazali,L., Hamamy,H., Valente,E.M., Gorman,S., Williams,R.,
            McHale,D.P., Wood,J.N., Gribble,F.M. and Woods,C.G.
  TITLE     An SCN9A channelopathy causes congenital inability to experience
            pain
  JOURNAL   Nature 444 (7121), 894-898 (2006)
   PUBMED   17167479
  REMARK    GeneRIF: data suggest that SCN9A is an essential and non-redundant
            requirement for nociception in humans
            GeneRIF: An SCN9A channelopathy causes congenital inability to
            experience pain
REFERENCE   5  (bases 1 to 9752)
  AUTHORS   Plummer,N.W. and Meisler,M.H.
  TITLE     Evolution and diversity of mammalian sodium channel genes
  JOURNAL   Genomics 57 (2), 323-331 (1999)
   PUBMED   10198179
  REMARK    Review article
REFERENCE   6  (bases 1 to 9752)
  AUTHORS   Sangameswaran,L., Fish,L.M., Koch,B.D., Rabert,D.K., Delgado,S.G.,
            Ilnicka,M., Jakeman,L.B., Novakovic,S., Wong,K., Sze,P.,
            Tzoumaka,E., Stewart,G.R., Herman,R.C., Chan,H., Eglen,R.M. and
            Hunter,J.C.
  TITLE     A novel tetrodotoxin-sensitive, voltage-gated sodium channel
            expressed in rat and human dorsal root ganglia
  JOURNAL   J Biol Chem 272 (23), 14805-14809 (1997)
   PUBMED   9169448
REFERENCE   7  (bases 1 to 9752)
  AUTHORS   Klugbauer,N., Lacinova,L., Flockerzi,V. and Hofmann,F.
  TITLE     Structure and functional expression of a new member of the
            tetrodotoxin-sensitive voltage-activated sodium channel family from
            human neuroendocrine cells
  JOURNAL   EMBO J 14 (6), 1084-1090 (1995)
   PUBMED   7720699
REFERENCE   8  (bases 1 to 9752)
  AUTHORS   Schon,K.R., Parker,A.P.J. and Woods,C.G.
  TITLE     Congenital Insensitivity to Pain Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   29419974
REFERENCE   9  (bases 1 to 9752)
  AUTHORS   Kurth,I.
  TITLE     Hereditary Sensory and Autonomic Neuropathy Type II
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   21089229
REFERENCE   10 (bases 1 to 9752)
  AUTHORS   Hisama,F.M., Dib-Hajj,S.D. and Waxman,S.G.
  TITLE     SCN9A Neuropathic Pain Syndromes
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301342
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC074101.5, AC108146.3 and
            AC107082.3.
            
            On Aug 31, 2018 this sequence version replaced XM_005246757.2.
            
            Summary: This gene encodes a voltage-gated sodium channel which
            plays a significant role in nociception signaling. Mutations in
            this gene have been associated with primary erythermalgia,
            channelopathy-associated insensitivity to pain, and paroxysmal
            extreme pain disorder. [provided by RefSeq, Aug 2009].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803613.201250.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000642356.2/ ENSP00000495601.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-248               AC074101.5         41540-41787         c
            249-556             AC108146.3         67152-67459         c
            557-675             AC108146.3         62609-62727         c
            676-765             AC108146.3         62163-62252         c
            766-894             AC108146.3         61445-61573         c
            895-986             AC108146.3         59891-59982         c
            987-1199            AC108146.3         58743-58955         c
            1200-1263           AC108146.3         50252-50315         c
            1264-1405           AC108146.3         48884-49025         c
            1406-1612           AC108146.3         44090-44296         c
            1613-1900           AC108146.3         41989-42276         c
            1901-2272           AC108146.3         40106-40477         c
            2273-2402           AC108146.3         37332-37461         c
            2403-2641           AC108146.3         36010-36248         c
            2642-2815           AC108146.3         33793-33966         c
            2816-3172           AC108146.3         32636-32992         c
            3173-3649           AC108146.3         28052-28528         c
            3650-3770           AC108146.3         7418-7538           c
            3771-3925           AC107082.3         109099-109253       c
            3926-4099           AC107082.3         104691-104864       c
            4100-4222           AC107082.3         99937-100059        c
            4223-4504           AC107082.3         95288-95569         c
            4505-4558           AC107082.3         94267-94320         c
            4559-4696           AC107082.3         93164-93301         c
            4697-4801           AC107082.3         70957-71061         c
            4802-5072           AC107082.3         70552-70822         c
            5073-9752           AC107082.3         61782-66461         c
FEATURES             Location/Qualifiers
     source          1..9752
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q24.3"
     gene            1..9752
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; NE-NA;
                     NENA; PN1; SFNP"
                     /note="sodium voltage-gated channel alpha subunit 9"
                     /db_xref="GeneID:6335"
                     /db_xref="HGNC:HGNC:10597"
                     /db_xref="MIM:603415"
     exon            1..248
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; NE-NA;
                     NENA; PN1; SFNP"
                     /inference="alignment:Splign:2.1.0"
     exon            249..556
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; NE-NA;
                     NENA; PN1; SFNP"
                     /inference="alignment:Splign:2.1.0"
     CDS             299..6265
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; NE-NA;
                     NENA; PN1; SFNP"
                     /note="isoform 2 is encoded by transcript variant 2;
                     sodium channel, voltage-gated, type IX, alpha polypeptide;
                     sodium channel protein type 9 subunit alpha; sodium
                     channel, voltage-gated, type IX, alpha subunit;
                     voltage-gated sodium channel alpha subunit Nav1.7; hNE-Na;
                     peripheral sodium channel 1; neuroendocrine sodium
                     channel; sodium channel protein type IX subunit alpha;
                     voltage-gated sodium channel subunit alpha Nav1.7"
                     /codon_start=1
                     /product="sodium channel protein type 9 subunit alpha
                     isoform 2"
                     /protein_id="NP_001352465.1"
                     /db_xref="CCDS:CCDS92892.1"
                     /db_xref="GeneID:6335"
                     /db_xref="HGNC:HGNC:10597"
                     /db_xref="MIM:603415"
                     /translation="MAMLPPPGPQSFVHFTKQSLALIEQRIAERKSKEPKEEKKDDDE
                     EAPKPSSDLEAGKQLPFIYGDIPPGMVSEPLEDLDPYYADKKTFIVLNKGKTIFRFNA
                     TPALYMLSPFSPLRRISIKILVHSLFSMLIMCTILTNCIFMTMNNPPDWTKNVEYTFT
                     GIYTFESLVKILARGFCVGEFTFLRDPWNWLDFVVIVFAYLTEFVNLGNVSALRTFRV
                     LRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLKHKCF
                     RNSLENNETLESIMNTLESEEDFRKYFYYLEGSKDALLCGFSTDSGQCPEGYTCVKIG
                     RNPDYGYTSFDTFSWAFLALFRLMTQDYWENLYQQTLRAAGKTYMIFFVVVIFLGSFY
                     LINLILAVVAMAYEEQNQANIEEAKQKELEFQQMLDRLKKEQEEAEAIAAAAAEYTSI
                     RRSRIMGLSESSSETSKLSSKSAKERRNRRKKKNQKKLSSGEEKGDAEKLSKSESEDS
                     IRRKSFHLGVEGHRRAHEKRLSTPNQSPLSIRGSLFSARRSSRTSLFSFKGRGRDIGS
                     ETEFADDEHSIFGDNESRRGSLFVPHRPQERRSSNISQASRSPPMLPVNGKMHSAVDC
                     NGVVSLVDGRSALMLPNGQLLPEVIIDKATSDDSGTTNQIHKKRRCSSYLLSEDMLND
                     PNLRQRAMSRASILTNTVEELEESRQKCPPWWYRFAHKFLIWNCSPYWIKFKKCIYFI
                     VMDPFVDLAITICIVLNTLFMAMEHHPMTEEFKNVLAIGNLVFTGIFAAEMVLKLIAM
                     DPYEYFQVGWNIFDSLIVTLSLVELFLADVEGLSVLRSFRLLRVFKLAKSWPTLNMLI
                     KIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKINDDCTLPRWHMNDFF
                     HSFLIVFRVLCGEWIETMWDCMEVAGQAMCLIVYMMVMVIGNLVVLNLFLALLLSSFS
                     SDNLTAIEEDPDANNLQIAVTRIKKGINYVKQTLREFILKAFSKKPKISREIRQAEDL
                     NTKKENYISNHTLAEMSKGHNFLKEKDKISGFGSSVDKHLMEDSDGQSFIHNPSLTVT
                     VPIAPGESDLENMNAEELSSDSDSEYSKVRLNRSSSSECSTVDNPLPGEGEEAEAEPM
                     NSDEPEACFTDGCVWRFSCCQVNIESGKGKIWWNIRKTCYKIVEHSWFESFIVLMILL
                     SSGALAFEDIYIERKKTIKIILEYADKIFTYIFILEMLLKWIAYGYKTYFTNAWCWLD
                     FLIVDVSLVTLVANTLGYSDLGPIKSLRTLRALRPLRALSRFEGMRVVVNALIGAIPS
                     IMNVLLVCLIFWLIFSIMGVNLFAGKFYECINTTDGSRFPASQVPNRSECFALMNVSQ
                     NVRWKNLKVNFDNVGLGYLSLLQVATFKGWTIIMYAAVDSVNVDKQPKYEYSLYMYIY
                     FVVFIIFGSFFTLNLFIGVIIDNFNQQKKKLGGQDIFMTEEQKKYYNAMKKLGSKKPQ
                     KPIPRPGNKIQGCIFDLVTNQAFDISIMVLICLNMVTMMVEKEGQSQHMTEVLYWINV
                     VFIILFTGECVLKLISLRHYYFTVGWNIFDFVVVIISIVGMFLADLIETYFVSPTLFR
                     VIRLARIGRILRLVKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAY
                     VKKEDGINDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPKKVHPGSSV
                     EGDCGNPSVGIFYFVSYIIISFLVVVNMYIAVILENFSVATEESTEPLSEDDFEMFYE
                     VWEKFDPDATQFIEFSKLSDFAAALDPPLLIAKPNKVQLIAMDLPMVSGDRIHCLDIL
                     FAFTKRVLGESGEMDSLRSQMEERFMSANPSKVSYEPITTTLKRKQEDVSATVIQRAY
                     RRYRLRQNVKNISSIYIKDGDRDDDLLNKKDMAFDNVNENSSPEKTDATSSTTSPPSY
                     DSVTKPDKEKYEQDRTEKEDKGKDSKESKK"
     exon            557..675
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; NE-NA;
                     NENA; PN1; SFNP"
                     /inference="alignment:Splign:2.1.0"
     exon            676..765
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; NE-NA;
                     NENA; PN1; SFNP"
                     /inference="alignment:Splign:2.1.0"
     exon            766..894
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; NE-NA;
                     NENA; PN1; SFNP"
                     /inference="alignment:Splign:2.1.0"
     exon            895..986
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; NE-NA;
                     NENA; PN1; SFNP"
                     /inference="alignment:Splign:2.1.0"
     exon            987..1199
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; NE-NA;
                     NENA; PN1; SFNP"
                     /inference="alignment:Splign:2.1.0"
     exon            1200..1263
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; NE-NA;
                     NENA; PN1; SFNP"
                     /inference="alignment:Splign:2.1.0"
     exon            1264..1405
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; NE-NA;
                     NENA; PN1; SFNP"
                     /inference="alignment:Splign:2.1.0"
     exon            1406..1612
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; NE-NA;
                     NENA; PN1; SFNP"
                     /inference="alignment:Splign:2.1.0"
     exon            1613..1900
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; NE-NA;
                     NENA; PN1; SFNP"
                     /inference="alignment:Splign:2.1.0"
     exon            1901..2272
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; NE-NA;
                     NENA; PN1; SFNP"
                     /inference="alignment:Splign:2.1.0"
     exon            2273..2402
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; NE-NA;
                     NENA; PN1; SFNP"
                     /inference="alignment:Splign:2.1.0"
     exon            2403..2641
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; NE-NA;
                     NENA; PN1; SFNP"
                     /inference="alignment:Splign:2.1.0"
     exon            2642..2815
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; NE-NA;
                     NENA; PN1; SFNP"
                     /inference="alignment:Splign:2.1.0"
     exon            2816..3172
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; NE-NA;
                     NENA; PN1; SFNP"
                     /inference="alignment:Splign:2.1.0"
     exon            3173..3649
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; NE-NA;
                     NENA; PN1; SFNP"
                     /inference="alignment:Splign:2.1.0"
     exon            3650..3770
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; NE-NA;
                     NENA; PN1; SFNP"
                     /inference="alignment:Splign:2.1.0"
     exon            3771..3925
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; NE-NA;
                     NENA; PN1; SFNP"
                     /inference="alignment:Splign:2.1.0"
     exon            3926..4099
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; NE-NA;
                     NENA; PN1; SFNP"
                     /inference="alignment:Splign:2.1.0"
     exon            4100..4222
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; NE-NA;
                     NENA; PN1; SFNP"
                     /inference="alignment:Splign:2.1.0"
     exon            4223..4504
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; NE-NA;
                     NENA; PN1; SFNP"
                     /inference="alignment:Splign:2.1.0"
     exon            4505..4558
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; NE-NA;
                     NENA; PN1; SFNP"
                     /inference="alignment:Splign:2.1.0"
     exon            4559..4696
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; NE-NA;
                     NENA; PN1; SFNP"
                     /inference="alignment:Splign:2.1.0"
     exon            4697..4801
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; NE-NA;
                     NENA; PN1; SFNP"
                     /inference="alignment:Splign:2.1.0"
     exon            4802..5072
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; NE-NA;
                     NENA; PN1; SFNP"
                     /inference="alignment:Splign:2.1.0"
     exon            5073..9752
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; NE-NA;
                     NENA; PN1; SFNP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6529..6534
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; NE-NA;
                     NENA; PN1; SFNP"
                     /note="hexamer: AATAAA"
     polyA_site      6554
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; NE-NA;
                     NENA; PN1; SFNP"
                     /note="major polyA site"
     regulatory      9730..9735
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; NE-NA;
                     NENA; PN1; SFNP"
                     /note="hexamer: AATAAA"
     polyA_site      9752
                     /gene="SCN9A"
                     /gene_synonym="ETHA; FEB3B; GEFSP7; HSAN2D; Nav1.7; NE-NA;
                     NENA; PN1; SFNP"
                     /note="major polyA site"
ORIGIN      
        1 agtctgcttg caggcggtcg ccagcgctcc agcggcggct gtcggctttc caattccgcc
       61 agctcggctg aggctgggct agcctgggtg ccagtggctg ctagcggcag gcgtcccctg
      121 agcaacagga gcccagagaa aaagaagcag ccctgagaga gcgccgggga aggagaggcc
      181 cgcgccctct cctggagcca gattctgcag gtgcactggg tggggatgat cggcgggcta
      241 ggttgcaagc ctcttatgtg aggagctgaa gaggaattaa aatatacagg atgaaaagat
      301 ggcaatgttg cctcccccag gacctcagag ctttgtccat ttcacaaaac agtctcttgc
      361 cctcattgaa caacgcattg ctgaaagaaa atcaaaggaa cccaaagaag aaaagaaaga
      421 tgatgatgaa gaagccccaa agccaagcag tgacttggaa gctggcaaac agctgccctt
      481 catctatggg gacattcctc ccggcatggt gtcagagccc ctggaggact tggaccccta
      541 ctatgcagac aaaaagactt tcatagtatt gaacaaaggg aaaacaatct tccgtttcaa
      601 tgccacacct gctttatata tgctttctcc tttcagtcct ctaagaagaa tatctattaa
      661 gattttagta cactccttat tcagcatgct catcatgtgc actattctga caaactgcat
      721 atttatgacc atgaataacc caccggactg gaccaaaaat gtcgagtaca cttttactgg
      781 aatatatact tttgaatcac ttgtaaaaat ccttgcaaga ggcttctgtg taggagaatt
      841 cacttttctt cgtgacccgt ggaactggct ggattttgtc gtcattgttt ttgcgtattt
      901 aacagaattt gtaaacctag gcaatgtttc agctcttcga actttcagag tattgagagc
      961 tttgaaaact atttctgtaa tcccaggcct gaagacaatt gtaggggctt tgatccagtc
     1021 agtgaagaag ctttctgatg tcatgatcct gactgtgttc tgtctgagtg tgtttgcact
     1081 aattggacta cagctgttca tgggaaacct gaagcataaa tgttttcgaa attcacttga
     1141 aaataatgaa acattagaaa gcataatgaa taccctagag agtgaagaag actttagaaa
     1201 atatttttat tacttggaag gatccaaaga tgctctcctt tgtggtttca gcacagattc
     1261 aggtcagtgt ccagaggggt acacctgtgt gaaaattggc agaaaccctg attatggcta
     1321 cacgagcttt gacactttca gctgggcctt cttagccttg tttaggctaa tgacccaaga
     1381 ttactgggaa aacctttacc aacagacgct gcgtgctgct ggcaaaacct acatgatctt
     1441 ctttgtcgta gtgattttcc tgggctcctt ttatctaata aacttgatcc tggctgtggt
     1501 tgccatggca tatgaagaac agaaccaggc aaacattgaa gaagctaaac agaaagaatt
     1561 agaatttcaa cagatgttag accgtcttaa aaaagagcaa gaagaagctg aggcaattgc
     1621 agcggcagcg gctgaatata caagtattag gagaagcaga attatgggcc tctcagagag
     1681 ttcttctgaa acatccaaac tgagctctaa aagtgctaaa gaaagaagaa acagaagaaa
     1741 gaaaaagaat caaaagaagc tctccagtgg agaggaaaag ggagatgctg agaaattgtc
     1801 gaaatcagaa tcagaggaca gcatcagaag aaaaagtttc caccttggtg tcgaagggca
     1861 taggcgagca catgaaaaga ggttgtctac ccccaatcag tcaccactca gcattcgtgg
     1921 ctccttgttt tctgcaaggc gaagcagcag aacaagtctt tttagtttca aaggcagagg
     1981 aagagatata ggatctgaga ctgaatttgc cgatgatgag cacagcattt ttggagacaa
     2041 tgagagcaga aggggctcac tgtttgtgcc ccacagaccc caggagcgac gcagcagtaa
     2101 catcagccaa gccagtaggt ccccaccaat gctgccggtg aacgggaaaa tgcacagtgc
     2161 tgtggactgc aacggtgtgg tctccctggt tgatggacgc tcagccctca tgctccccaa
     2221 tggacagctt ctgccagagg tgataataga taaggcaact tctgatgaca gcggcacgac
     2281 caatcaaata cacaagaaaa ggcgttgtag ttcctatctc ctttcagagg atatgctgaa
     2341 tgatcccaac ctcagacaga gagcaatgag tagagcaagc atattaacaa acactgtgga
     2401 agaacttgaa gagtccagac aaaaatgtcc accttggtgg tacagatttg cacacaaatt
     2461 cttgatctgg aattgctctc catattggat aaaattcaaa aagtgtatct attttattgt
     2521 aatggatcct tttgtagatc ttgcaattac catttgcata gttttaaaca cattatttat
     2581 ggctatggaa caccacccaa tgactgagga attcaaaaat gtacttgcta taggaaattt
     2641 ggtctttact ggaatctttg cagctgaaat ggtattaaaa ctgattgcca tggatccata
     2701 tgagtatttc caagtaggct ggaatatttt tgacagcctt attgtgactt taagtttagt
     2761 ggagctcttt ctagcagatg tggaaggatt gtcagttctg cgatcattca gactgctccg
     2821 agtcttcaag ttggcaaaat cctggccaac attgaacatg ctgattaaga tcattggtaa
     2881 ctcagtaggg gctctaggta acctcacctt agtgttggcc atcatcgtct tcatttttgc
     2941 tgtggtcggc atgcagctct ttggtaagag ctacaaagaa tgtgtctgca agatcaatga
     3001 tgactgtacg ctcccacggt ggcacatgaa cgacttcttc cactccttcc tgattgtgtt
     3061 ccgcgtgctg tgtggagagt ggatagagac catgtgggac tgtatggagg tcgctggtca
     3121 agctatgtgc cttattgttt acatgatggt catggtcatt ggaaacctgg tggtcctaaa
     3181 cctatttctg gccttattat tgagctcatt tagttcagac aatcttacag caattgaaga
     3241 agaccctgat gcaaacaacc tccagattgc agtgactaga attaaaaagg gaataaatta
     3301 tgtgaaacaa accttacgtg aatttattct aaaagcattt tccaaaaagc caaagatttc
     3361 cagggagata agacaagcag aagatctgaa tactaagaag gaaaactata tttctaacca
     3421 tacacttgct gaaatgagca aaggtcacaa tttcctcaag gaaaaagata aaatcagtgg
     3481 ttttggaagc agcgtggaca aacacttgat ggaagacagt gatggtcaat catttattca
     3541 caatcccagc ctcacagtga cagtgccaat tgcacctggg gaatccgatt tggaaaatat
     3601 gaatgctgag gaacttagca gtgattcgga tagtgaatac agcaaagtga gattaaaccg
     3661 gtcaagctcc tcagagtgca gcacagttga taaccctttg cctggagaag gagaagaagc
     3721 agaggctgaa cctatgaatt ccgatgagcc agaggcctgt ttcacagatg gttgtgtatg
     3781 gaggttctca tgctgccaag ttaacataga gtcagggaaa ggaaaaatct ggtggaacat
     3841 caggaaaacc tgctacaaga ttgttgaaca cagttggttt gaaagcttca ttgtcctcat
     3901 gatcctgctc agcagtggtg ccctggcttt tgaagatatt tatattgaaa ggaaaaagac
     3961 cattaagatt atcctggagt atgcagacaa gatcttcact tacatcttca ttctggaaat
     4021 gcttctaaaa tggatagcat atggttataa aacatatttc accaatgcct ggtgttggct
     4081 ggatttccta attgttgatg tttctttggt tactttagtg gcaaacactc ttggctactc
     4141 agatcttggc cccattaaat cccttcggac actgagagct ttaagacctc taagagcctt
     4201 atctagattt gaaggaatga gggtcgttgt gaatgcactc ataggagcaa ttccttccat
     4261 catgaatgtg ctacttgtgt gtcttatatt ctggctgata ttcagcatca tgggagtaaa
     4321 tttgtttgct ggcaagttct atgagtgtat taacaccaca gatgggtcac ggtttcctgc
     4381 aagtcaagtt ccaaatcgtt ccgaatgttt tgcccttatg aatgttagtc aaaatgtgcg
     4441 atggaaaaac ctgaaagtga actttgataa tgtcggactt ggttacctat ctctgcttca
     4501 agttgcaact tttaagggat ggacgattat tatgtatgca gcagtggatt ctgttaatgt
     4561 agacaagcag cccaaatatg aatatagcct ctacatgtat atttattttg tcgtctttat
     4621 catctttggg tcattcttca ctttgaactt gttcattggt gtcatcatag ataatttcaa
     4681 ccaacagaaa aagaagcttg gaggtcaaga catctttatg acagaagaac agaagaaata
     4741 ctataatgca atgaaaaagc tggggtccaa gaagccacaa aagccaattc ctcgaccagg
     4801 gaacaaaatc caaggatgta tatttgacct agtgacaaat caagcctttg atattagtat
     4861 catggttctt atctgtctca acatggtaac catgatggta gaaaaggagg gtcaaagtca
     4921 acatatgact gaagttttat attggataaa tgtggttttt ataatccttt tcactggaga
     4981 atgtgtgcta aaactgatct ccctcagaca ctactacttc actgtaggat ggaatatttt
     5041 tgattttgtg gttgtgatta tctccattgt aggtatgttt ctagctgatt tgattgaaac
     5101 gtattttgtg tcccctaccc tgttccgagt gatccgtctt gccaggattg gccgaatcct
     5161 acgtctagtc aaaggagcaa aggggatccg cacgctgctc tttgctttga tgatgtccct
     5221 tcctgcgttg tttaacatcg gcctcctgct cttcctggtc atgttcatct acgccatctt
     5281 tggaatgtcc aactttgcct atgttaaaaa ggaagatgga attaatgaca tgttcaattt
     5341 tgagaccttt ggcaacagta tgatttgcct gttccaaatt acaacctctg ctggctggga
     5401 tggattgcta gcacctattc ttaacagtaa gccacccgac tgtgacccaa aaaaagttca
     5461 tcctggaagt tcagttgaag gagactgtgg taacccatct gttggaatat tctactttgt
     5521 tagttatatc atcatatcct tcctggttgt ggtgaacatg tacattgcag tcatactgga
     5581 gaattttagt gttgccactg aagaaagtac tgaacctctg agtgaggatg actttgagat
     5641 gttctatgag gtttgggaga agtttgatcc cgatgcgacc cagtttatag agttctctaa
     5701 actctctgat tttgcagctg ccctggatcc tcctcttctc atagcaaaac ccaacaaagt
     5761 ccagctcatt gccatggatc tgcccatggt tagtggtgac cggatccatt gtcttgacat
     5821 cttatttgct tttacaaagc gtgttttggg tgagagtggg gagatggatt ctcttcgttc
     5881 acagatggaa gaaaggttca tgtctgcaaa tccttccaaa gtgtcctatg aacccatcac
     5941 aaccacacta aaacggaaac aagaggatgt gtctgctact gtcattcagc gtgcttatag
     6001 acgttaccgc ttaaggcaaa atgtcaaaaa tatatcaagt atatacataa aagatggaga
     6061 cagagatgat gatttactca ataaaaaaga tatggctttt gataatgtta atgagaactc
     6121 aagtccagaa aaaacagatg ccacttcatc caccacctct ccaccttcat atgatagtgt
     6181 aacaaagcca gacaaagaga aatatgaaca agacagaaca gaaaaggaag acaaagggaa
     6241 agacagcaag gaaagcaaaa aatagagctt catttttgat atattgttta cagcctgtga
     6301 aagtgattta tttgtgttaa taaaactctt ttgaggaagt ctatgccaaa atccttttta
     6361 tcaaaatatt ctcgaaggca gtgcagtcac taactctgat ttcctaagaa aggtgggcag
     6421 cattagcaga tggttatttt tgcactgatg attctttaag aatcgtaaga gaactctgta
     6481 ggaattattg attatagcat acaaaagtga ttcagttttt tggtttttaa taaatcagaa
     6541 gaccatgtag aaaactttta catctgcctt gtcatctttt cacaggattg taattagtct
     6601 tgtttcccat gtaaataaac aacacacgca tacagaaaaa tctattattt atctattatt
     6661 tggaaatcaa caaaagtatt tgccttggct ttgcaatgaa atgcttgata gaagtaatgg
     6721 acattagtta tgaatgttta gttaaaatgc attattaggg agcttgactt tttatcaatg
     6781 tacagaggtt attctatatt ttgaggtgct taaatttatt ctacattgca tcagaaccaa
     6841 tttatatgtg cctataaaat gccatgggat taaaaatata tgtaggctat tcatttctac
     6901 aaatgttttt cattcatctt gactcacatg ccaacaagga taagacttac ctttagagta
     6961 ttgtgtttca tagcctttct tctttcatat ccctttttgt tcatagaata accacagaac
     7021 ttgaaaaatt attctaagta catattacac tcctcaaaaa aaacaaagat aactgagaaa
     7081 aaagttattg acagaagttc tatttgctat tatttacata gcctaacatt tgactgtgct
     7141 gcccaaaata ctgataatag tctcttaaac tcttttgtca aattttcctg ctttcttatg
     7201 cagtattgtt tagtcatcct ttcgctgtaa gcaaagttga tgaaatcctt cctgatatgc
     7261 agttagttgt ttgaccacgg tacatacttg agcagataat aacttgggca cagtatttat
     7321 tgcatcactt gtatacaatc ccgtgtttgg caagctttca aatcatgtaa tatgacagac
     7381 tttacacaga tatgtgttta gtatgaataa aaaagcattg aaatagggat tcttgccaac
     7441 ttgctctctt gccaccaact tactttccta aattatggaa gtaatctttt ttggatatac
     7501 ttcaatgtat acaatgagga agatgtcacc ttctccttaa aattctatga tgtgaaatat
     7561 attttgcctc aatcaacaca gtaccatggg cttctaattt atcaagcaca tattcatttt
     7621 gcattagctg tagacatcta gttttttgaa aacacctatt aatagtaatt tgaaaagaaa
     7681 taaccataat gctttttttc gtgagtttat ttcaggaata tgagatcttt cttctataaa
     7741 gttattcatg cacaggcaaa aattgagcta cacaggtaga atgtagtttt acttagaaga
     7801 tttttgtggg aggttttgaa gcaaatatat aaaacaactt tcactaattt gctttccata
     7861 tttaaaaaat aataaattac atttatataa taaatgttta aagcacatat tttttgttgt
     7921 tctggcaatt taaaaagaaa gaggatttaa acgtacctat agaaacaaag atttatggtt
     7981 aaagaatgag atcagaagtc tagaatgttt ttaaattgtg atatatttta caacatccgt
     8041 tattactttg agacatttgt cctaatctac gtataaaact caatctaggg ctaaagattc
     8101 tttataccat cttaggttca ttcatcttag gctatttgaa ccacttttta atttaatatg
     8161 aaagacacca tgcagtgttt tccgagacta catagatcat tttatcacat acctaccaag
     8221 cctgttggaa ataggttttg ataatttaag tagggaccta tacaaaatat attacattta
     8281 tcagattttt aaatacattc aattaagaat ttaacatcac cttaaatttg aattcaatct
     8341 accgttattt caaactcaca aatataactg cattatgaat acttacataa tgtagtaaga
     8401 caagatgttt gacaggttcg tgtgtaattt tctattaatg tttttacatt gccttgtttt
     8461 tatgtaaaat aaaaaatatg ggcaactggt ttgttaacaa cacaatttct tcttagcatt
     8521 tcaaaaatat atataaagtt gttctttttc ctatttcatg aactatgttt ttttttaaaa
     8581 taacatggtt aagttttata tatatttacg tttgtttcag gaatgtctac ttgtgacttt
     8641 ttatcaatta aaaataatat ttggaagaaa gagcttatta agtataagct tgaagtaaaa
     8701 ttagacctct ctttccatgt agattactgt ttgtactgat ggtttcaccc ttcagaaggc
     8761 actgtcatat taatatttaa attttataat cgctgaactt attacaccca acaatacaga
     8821 aaggcagtta cactgaagaa cttaacttag aataaaatgg aagcaaacag gttttctaaa
     8881 aactttttta agtgaccagg tctcgctctg tcacccaggc tagagtgcaa tggcatgatc
     8941 atagctctct gcagcctcaa ctctgggctc aagcaaccct cctgcctcag cctcccaagt
     9001 agctaagact acaggtacat gccaccatgc ctggctaata tttaaatttt tgtagataag
     9061 gggtcttgct atgttgccca ggctagtctc aaactcctgg cttcaagtgt tcctactgtc
     9121 atgacctgcc aacatgctgg ggttacaggc atgagccacc atgccccaaa caggtttgaa
     9181 cacaaatctt tcggatgaaa attagagaac ctaattttag ctttttgata gttacctagt
     9241 ttgcaaaaga tttgggtgac ttgtgagctg tttttaaatg ctgattgttg aacatcacaa
     9301 cccaaaatac ttagcatgat tttatagagt tttgatagct ttattaaaaa gagtgaaaat
     9361 aaaatgcata tgtaaataaa gcagttctaa atagctattt cagagaaatg ttaatagaag
     9421 tgctgaaaga agggccaact aaattaggat ggccagggaa ttggcctggg tttaggacct
     9481 atgtatgaag gccaccaatt ttttaaaaat atctgtggtt tattatgtta ttatcttctt
     9541 gaggaaaaca atcaagaatt gcttcatgaa aataaataaa tagccatgaa tatcataaag
     9601 ctgtttacat aggattcttt acaaatttca tagatctatg aatgctcaaa atgtttgagt
     9661 ttgccataaa ttatattgta gttatattgt agttatactt gagactgaca cattgtaata
     9721 taatctaaga ataaaagtta tacaaaataa aa
//
