LOCUS       NM_001330260           11559 bp    mRNA    linear   PRI 12-NOV-2024
DEFINITION  Homo sapiens sodium voltage-gated channel alpha subunit 8 (SCN8A),
            transcript variant 3, mRNA.
ACCESSION   NM_001330260 XM_011538650
VERSION     NM_001330260.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 11559)
  AUTHORS   Miralles,R.M., Boscia,A.R., Kittur,S., Hanflink,J.C., Panchal,P.S.,
            Yorek,M.S., Deutsch,T.C.J., Reever,C.M., Vundela,S.R., Wengert,E.R.
            and Patel,M.K.
  TITLE     Parvalbumin interneuron impairment causes synaptic transmission
            deficits and seizures in SCN8A developmental and epileptic
            encephalopathy
  JOURNAL   JCI Insight 9 (20), e181005 (2024)
   PUBMED   39435659
  REMARK    GeneRIF: Parvalbumin interneuron impairment causes synaptic
            transmission deficits and seizures in SCN8A developmental and
            epileptic encephalopathy.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11559)
  AUTHORS   Zhang,X., Qiu,S., Yang,L., Li,Y., Xu,N. and Yu,X.
  TITLE     [Clinical features and genetic analysis of five children with
            epilepsies due to variants of SCN8A gene]
  JOURNAL   Zhonghua Yi Xue Yi Chuan Xue Za Zhi 41 (2), 174-180 (2024)
   PUBMED   38311555
  REMARK    GeneRIF: [Clinical features and genetic analysis of five children
            with epilepsies due to variants of SCN8A gene].
REFERENCE   3  (bases 1 to 11559)
  AUTHORS   Yuan,T., Wang,Y., Jin,Y., Yang,H., Xu,S., Zhang,H., Chen,Q., Li,N.,
            Ma,X., Song,H., Peng,C., Geng,Z., Dong,J., Duan,G., Sun,Q.,
            Yang,Y., Yang,F. and Huang,Z.
  TITLE     Coupling of Slack and NaV1.6 sensitizes Slack to quinidine blockade
            and guides anti-seizure strategy development
  JOURNAL   Elife 12, 87559 (2024)
   PUBMED   38289338
  REMARK    GeneRIF: Coupling of Slack and NaV1.6 sensitizes Slack to quinidine
            blockade and guides anti-seizure strategy development.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 11559)
  AUTHORS   Hebbar,M., Al-Taweel,N., Gill,I., Boelman,C., Dean,R.A.,
            Goodchild,S.J., Mezeyova,J., Shuart,N.G., Johnson,J.P. Jr., Lee,J.,
            Michoulas,A., Huh,L.L., Armstrong,L., Connolly,M.B. and Demos,M.K.
  TITLE     Expanding the genotype-phenotype spectrum in SCN8A-related
            disorders
  JOURNAL   BMC Neurol 24 (1), 31 (2024)
   PUBMED   38233770
  REMARK    GeneRIF: Expanding the genotype-phenotype spectrum in SCN8A-related
            disorders.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 11559)
  AUTHORS   Chung,K.M., Hack,J., Andrews,J., Galindo-Kelly,M., Schreiber,J.,
            Watkins,J. and Hammer,M.F.
  TITLE     Clinical severity is correlated with age at seizure onset and
            biophysical properties of recurrent gain of function variants
            associated with SCN8A-related epilepsy
  JOURNAL   Epilepsia 64 (12), 3365-3376 (2023)
   PUBMED   37585367
  REMARK    GeneRIF: Clinical severity is correlated with age at seizure onset
            and biophysical properties of recurrent gain of function variants
            associated with SCN8A-related epilepsy.
REFERENCE   6  (bases 1 to 11559)
  AUTHORS   Plummer,N.W., Galt,J., Jones,J.M., Burgess,D.L., Sprunger,L.K.,
            Kohrman,D.C. and Meisler,M.H.
  TITLE     Exon organization, coding sequence, physical mapping, and
            polymorphic intragenic markers for the human neuronal sodium
            channel gene SCN8A
  JOURNAL   Genomics 54 (2), 287-296 (1998)
   PUBMED   9828131
REFERENCE   7  (bases 1 to 11559)
  AUTHORS   Plummer,N.W., McBurney,M.W. and Meisler,M.H.
  TITLE     Alternative splicing of the sodium channel SCN8A predicts a
            truncated two-domain protein in fetal brain and non-neuronal cells
  JOURNAL   J Biol Chem 272 (38), 24008-24015 (1997)
   PUBMED   9295353
REFERENCE   8  (bases 1 to 11559)
  AUTHORS   Genini,M., Schwalbe,P., Scholl,F.A. and Schafer,B.W.
  TITLE     Isolation of genes differentially expressed in human primary
            myoblasts and embryonal rhabdomyosarcoma
  JOURNAL   Int J Cancer 66 (4), 571-577 (1996)
   PUBMED   8635876
REFERENCE   9  (bases 1 to 11559)
  AUTHORS   Burgess,D.L., Kohrman,D.C., Galt,J., Plummer,N.W., Jones,J.M.,
            Spear,B. and Meisler,M.H.
  TITLE     Mutation of a new sodium channel gene, Scn8a, in the mouse mutant
            'motor endplate disease'
  JOURNAL   Nat Genet 10 (4), 461-465 (1995)
   PUBMED   7670495
REFERENCE   10 (bases 1 to 11559)
  AUTHORS   Hammer,M.F., Xia,M. and Schreiber,J.M.
  TITLE     SCN8A-Related Epilepsy and/or Neurodevelopmental Disorders
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   27559564
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC025097.41, AC140060.3,
            AC013421.31 and AC068987.38.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Apr 18, 2019 this sequence version replaced NM_001330260.1.
            
            Summary: This gene encodes a member of the sodium channel alpha
            subunit gene family. The encoded protein forms the ion pore region
            of the voltage-gated sodium channel. This protein is essential for
            the rapid membrane depolarization that occurs during the formation
            of the action potential in excitable neurons. Mutations in this
            gene are associated with cognitive disability, pancerebellar
            atrophy and ataxia. Alternate splicing results in multiple
            transcript variants.[provided by RefSeq, May 2010].
            
            Transcript Variant: This variant (3) contains an alternate in-frame
            exon in the 5' coding region, compared to variant 1. Isoforms 1 and
            3 are the same length but differ in their sequence.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMEA2154665,
                              SAMN03465403 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000627620.5/ ENSP00000487583.2
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-127               AC025097.41        104101-104227       c
            128-457             AC025097.41        32367-32696         c
            458-576             AC025097.41        11168-11286         c
            577-666             AC025097.41        9003-9092           c
            667-795             AC025097.41        8241-8369           c
            796-887             AC025097.41        6364-6455           c
            888-1109            AC140060.3         6115-6336
            1110-1173           AC140060.3         7689-7752
            1174-1315           AC140060.3         9318-9459
            1316-1522           AC140060.3         11962-12168
            1523-1816           AC140060.3         12967-13260
            1817-2179           AC140060.3         28091-28453
            2180-2312           AC013421.31        54187-54319         c
            2313-2551           AC013421.31        48629-48867         c
            2552-2725           AC013421.31        37546-37719         c
            2726-3082           AC013421.31        34195-34551         c
            3083-3553           AC013421.31        30887-31357         c
            3554-3671           AC013421.31        30237-30354         c
            3672-3826           AC013421.31        29539-29693         c
            3827-4000           AC013421.31        25860-26033         c
            4001-4123           AC013421.31        19451-19573         c
            4124-4408           AC013421.31        13396-13680         c
            4409-4462           AC013421.31        11474-11527         c
            4463-4600           AC013421.31        10804-10941         c
            4601-4705           AC013421.31        9720-9824           c
            4706-4976           AC013421.31        5581-5851           c
            4977-11559          AC068987.38        61017-67599         c
FEATURES             Location/Qualifiers
     source          1..11559
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q13.13"
     gene            1..11559
                     /gene="SCN8A"
                     /gene_synonym="BFIS5; CERIII; CIAT; DEE13; EIEE13; MED;
                     MYOCL2; NaCh6; Nav1.6; PN4"
                     /note="sodium voltage-gated channel alpha subunit 8"
                     /db_xref="GeneID:6334"
                     /db_xref="HGNC:HGNC:10596"
                     /db_xref="MIM:600702"
     exon            1..127
                     /gene="SCN8A"
                     /gene_synonym="BFIS5; CERIII; CIAT; DEE13; EIEE13; MED;
                     MYOCL2; NaCh6; Nav1.6; PN4"
                     /inference="alignment:Splign:2.1.0"
     exon            128..457
                     /gene="SCN8A"
                     /gene_synonym="BFIS5; CERIII; CIAT; DEE13; EIEE13; MED;
                     MYOCL2; NaCh6; Nav1.6; PN4"
                     /inference="alignment:Splign:2.1.0"
     CDS             182..6124
                     /gene="SCN8A"
                     /gene_synonym="BFIS5; CERIII; CIAT; DEE13; EIEE13; MED;
                     MYOCL2; NaCh6; Nav1.6; PN4"
                     /note="isoform 3 is encoded by transcript variant 3;
                     sodium channel protein type 8 subunit alpha; hNa6/Scn8a
                     voltage-gated sodium channel; voltage-gated sodium channel
                     subunit alpha Nav1.6; sodium channel, voltage gated, type
                     VIII, alpha subunit; voltage-gated sodium channel type
                     VIII alpha protein"
                     /codon_start=1
                     /product="sodium channel protein type 8 subunit alpha
                     isoform 3"
                     /protein_id="NP_001317189.1"
                     /db_xref="CCDS:CCDS81692.1"
                     /db_xref="GeneID:6334"
                     /db_xref="HGNC:HGNC:10596"
                     /db_xref="MIM:600702"
                     /translation="MAARLLAPPGPDSFKPFTPESLANIERRIAESKLKKPPKADGSH
                     REDDEDSKPKPNSDLEAGKSLPFIYGDIPQGLVAVPLEDFDPYYLTQKTFVVLNRGKT
                     LFRFSATPALYILSPFNLIRRIAIKILIHSVFSMIIMCTILTNCVFMTFSNPPDWSKN
                     VEYTFTGIYTFESLVKIIARGFCIDGFTFLRDPWNWLDFSVIMMAYVTEFVDLGNVSA
                     LRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGN
                     LRNKCVVWPINFNESYLENGTKGFDWEEYINNKTNFYTVPGMLEPLLCGNSSDAGQCP
                     EGYQCMKAGRNPNYGYTSFDTFSWAFLALFRLMTQDYWENLYQLTLRAAGKTYMIFFV
                     LVIFVGSFYLVNLILAVVAMAYEEQNQATLEEAEQKEAEFKAMLEQLKKQQEEAQAAA
                     MATSAGTVSEDAIEEEGEEGGGSPRSSSEISKLSSKSAKERRNRRKKRKQKELSEGEE
                     KGDPEKVFKSESEDGMRRKAFRLPDNRIGRKFSIMNQSLLSIPGSPFLSRHNSKSSIF
                     SFRGPGRFRDPGSENEFADDEHSTVEESEGRRDSLFIPIRARERRSSYSGYSGYSQGS
                     RSSRIFPSLRRSVKRNSTVDCNGVVSLIGGPGSHIGGRLLPEATTEVEIKKKGPGSLL
                     VSMDQLASYGRKDRINSIMSVVTNTLVEELEESQRKCPPCWYKFANTFLIWECHPYWI
                     KLKEIVNLIVMDPFVDLAITICIVLNTLFMAMEHHPMTPQFEHVLAVGNLVFTGIFTA
                     EMFLKLIAMDPYYYFQEGWNIFDGFIVSLSLMELSLADVEGLSVLRSFRLLRVFKLAK
                     SWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKINQDCEL
                     PRWHMHDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLIVFMMVMVIGNLVVLNLF
                     LALLLSSFSADNLAATDDDGEMNNLQISVIRIKKGVAWTKLKVHAFMQAHFKQREADE
                     VKPLDELYEKKANCIANHTGADIHRNGDFQKNGNGTTSGIGSSVEKYIIDEDHMSFIN
                     NPNLTVRVPIAVGESDFENLNTEDVSSESDPEGSKDKLDDTSSSEGSTIDIKPEVEEV
                     PVEQPEEYLDPDACFTEGCVQRFKCCQVNIEEGLGKSWWILRKTCFLIVEHNWFETFI
                     IFMILLSSGALAFEDIYIEQRKTIRTILEYADKVFTYIFILEMLLKWTAYGFVKFFTN
                     AWCWLDFLIVAVSLVSLIANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNAL
                     VGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKYHYCFNETSEIRFEIEDVNNKTECEK
                     LMEGNNTEIRWKNVKINFDNVGAGYLALLQVATFKGWMDIMYAAVDSRKPDEQPKYED
                     NIYMYIYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKK
                     LGSKKPQKPIPRPLNKIQGIVFDFVTQQAFDIVIMMLICLNMVTMMVETDTQSKQMEN
                     ILYWINLVFVIFFTCECVLKMFALRHYYFTIGWNIFDFVVVILSIVGMFLADIIEKYF
                     VSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIFSIF
                     GMSNFAYVKHEAGIDDMFNFETFGNSMICLFQITTSAGWDGLLLPILNRPPDCSLDKE
                     HPGSGFKGDCGNPSVGIFFFVSYIIISFLIVVNMYIAIILENFSVATEESADPLSEDD
                     FETFYEIWEKFDPDATQFIEYCKLADFADALEHPLRVPKPNTIELIAMDLPMVSGDRI
                     HCLDILFAFTKRVLGDSGELDILRQQMEERFVASNPSKVSYEPITTTLRRKQEEVSAV
                     VLQRAYRGHLARRGFICKKTTSNKLENGGTHREKKESTPSTASLPSYDSVTKPEKEKQ
                     QRAEEGRRERAKRQKEVRESKC"
     exon            458..576
                     /gene="SCN8A"
                     /gene_synonym="BFIS5; CERIII; CIAT; DEE13; EIEE13; MED;
                     MYOCL2; NaCh6; Nav1.6; PN4"
                     /inference="alignment:Splign:2.1.0"
     exon            577..666
                     /gene="SCN8A"
                     /gene_synonym="BFIS5; CERIII; CIAT; DEE13; EIEE13; MED;
                     MYOCL2; NaCh6; Nav1.6; PN4"
                     /inference="alignment:Splign:2.1.0"
     exon            667..795
                     /gene="SCN8A"
                     /gene_synonym="BFIS5; CERIII; CIAT; DEE13; EIEE13; MED;
                     MYOCL2; NaCh6; Nav1.6; PN4"
                     /inference="alignment:Splign:2.1.0"
     exon            796..887
                     /gene="SCN8A"
                     /gene_synonym="BFIS5; CERIII; CIAT; DEE13; EIEE13; MED;
                     MYOCL2; NaCh6; Nav1.6; PN4"
                     /inference="alignment:Splign:2.1.0"
     exon            888..1109
                     /gene="SCN8A"
                     /gene_synonym="BFIS5; CERIII; CIAT; DEE13; EIEE13; MED;
                     MYOCL2; NaCh6; Nav1.6; PN4"
                     /inference="alignment:Splign:2.1.0"
     exon            1110..1173
                     /gene="SCN8A"
                     /gene_synonym="BFIS5; CERIII; CIAT; DEE13; EIEE13; MED;
                     MYOCL2; NaCh6; Nav1.6; PN4"
                     /inference="alignment:Splign:2.1.0"
     exon            1174..1315
                     /gene="SCN8A"
                     /gene_synonym="BFIS5; CERIII; CIAT; DEE13; EIEE13; MED;
                     MYOCL2; NaCh6; Nav1.6; PN4"
                     /inference="alignment:Splign:2.1.0"
     exon            1316..1522
                     /gene="SCN8A"
                     /gene_synonym="BFIS5; CERIII; CIAT; DEE13; EIEE13; MED;
                     MYOCL2; NaCh6; Nav1.6; PN4"
                     /inference="alignment:Splign:2.1.0"
     exon            1523..1816
                     /gene="SCN8A"
                     /gene_synonym="BFIS5; CERIII; CIAT; DEE13; EIEE13; MED;
                     MYOCL2; NaCh6; Nav1.6; PN4"
                     /inference="alignment:Splign:2.1.0"
     exon            1817..2179
                     /gene="SCN8A"
                     /gene_synonym="BFIS5; CERIII; CIAT; DEE13; EIEE13; MED;
                     MYOCL2; NaCh6; Nav1.6; PN4"
                     /inference="alignment:Splign:2.1.0"
     exon            2180..2312
                     /gene="SCN8A"
                     /gene_synonym="BFIS5; CERIII; CIAT; DEE13; EIEE13; MED;
                     MYOCL2; NaCh6; Nav1.6; PN4"
                     /inference="alignment:Splign:2.1.0"
     exon            2313..2551
                     /gene="SCN8A"
                     /gene_synonym="BFIS5; CERIII; CIAT; DEE13; EIEE13; MED;
                     MYOCL2; NaCh6; Nav1.6; PN4"
                     /inference="alignment:Splign:2.1.0"
     exon            2552..2725
                     /gene="SCN8A"
                     /gene_synonym="BFIS5; CERIII; CIAT; DEE13; EIEE13; MED;
                     MYOCL2; NaCh6; Nav1.6; PN4"
                     /inference="alignment:Splign:2.1.0"
     exon            2726..3082
                     /gene="SCN8A"
                     /gene_synonym="BFIS5; CERIII; CIAT; DEE13; EIEE13; MED;
                     MYOCL2; NaCh6; Nav1.6; PN4"
                     /inference="alignment:Splign:2.1.0"
     exon            3083..3553
                     /gene="SCN8A"
                     /gene_synonym="BFIS5; CERIII; CIAT; DEE13; EIEE13; MED;
                     MYOCL2; NaCh6; Nav1.6; PN4"
                     /inference="alignment:Splign:2.1.0"
     exon            3554..3671
                     /gene="SCN8A"
                     /gene_synonym="BFIS5; CERIII; CIAT; DEE13; EIEE13; MED;
                     MYOCL2; NaCh6; Nav1.6; PN4"
                     /inference="alignment:Splign:2.1.0"
     exon            3672..3826
                     /gene="SCN8A"
                     /gene_synonym="BFIS5; CERIII; CIAT; DEE13; EIEE13; MED;
                     MYOCL2; NaCh6; Nav1.6; PN4"
                     /inference="alignment:Splign:2.1.0"
     exon            3827..4000
                     /gene="SCN8A"
                     /gene_synonym="BFIS5; CERIII; CIAT; DEE13; EIEE13; MED;
                     MYOCL2; NaCh6; Nav1.6; PN4"
                     /inference="alignment:Splign:2.1.0"
     exon            4001..4123
                     /gene="SCN8A"
                     /gene_synonym="BFIS5; CERIII; CIAT; DEE13; EIEE13; MED;
                     MYOCL2; NaCh6; Nav1.6; PN4"
                     /inference="alignment:Splign:2.1.0"
     exon            4124..4408
                     /gene="SCN8A"
                     /gene_synonym="BFIS5; CERIII; CIAT; DEE13; EIEE13; MED;
                     MYOCL2; NaCh6; Nav1.6; PN4"
                     /inference="alignment:Splign:2.1.0"
     exon            4409..4462
                     /gene="SCN8A"
                     /gene_synonym="BFIS5; CERIII; CIAT; DEE13; EIEE13; MED;
                     MYOCL2; NaCh6; Nav1.6; PN4"
                     /inference="alignment:Splign:2.1.0"
     exon            4463..4600
                     /gene="SCN8A"
                     /gene_synonym="BFIS5; CERIII; CIAT; DEE13; EIEE13; MED;
                     MYOCL2; NaCh6; Nav1.6; PN4"
                     /inference="alignment:Splign:2.1.0"
     exon            4601..4705
                     /gene="SCN8A"
                     /gene_synonym="BFIS5; CERIII; CIAT; DEE13; EIEE13; MED;
                     MYOCL2; NaCh6; Nav1.6; PN4"
                     /inference="alignment:Splign:2.1.0"
     exon            4706..4976
                     /gene="SCN8A"
                     /gene_synonym="BFIS5; CERIII; CIAT; DEE13; EIEE13; MED;
                     MYOCL2; NaCh6; Nav1.6; PN4"
                     /inference="alignment:Splign:2.1.0"
     exon            4977..11559
                     /gene="SCN8A"
                     /gene_synonym="BFIS5; CERIII; CIAT; DEE13; EIEE13; MED;
                     MYOCL2; NaCh6; Nav1.6; PN4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      11525..11530
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SCN8A"
                     /gene_synonym="BFIS5; CERIII; CIAT; DEE13; EIEE13; MED;
                     MYOCL2; NaCh6; Nav1.6; PN4"
                     /note="hexamer: AATAAA"
     polyA_site      11559
                     /gene="SCN8A"
                     /gene_synonym="BFIS5; CERIII; CIAT; DEE13; EIEE13; MED;
                     MYOCL2; NaCh6; Nav1.6; PN4"
                     /note="major polyA site"
ORIGIN      
        1 ggggagcgct ccaagatggc gcccaccgca gtcccgcccg ccgcatcctc ggcgcctttg
       61 cagtccggcc gcgcctcccg ggccccgcgt tagggccgcc gctgcctccc tcgccgccgc
      121 cgctgccagc tgacctgtcc tggacgcagc ataactaacg aagctgctgc aggatgagaa
      181 gatggcagcg cggctgcttg caccaccagg ccctgatagt ttcaagcctt tcacccctga
      241 gtcactggca aacattgaga ggcgcattgc tgagagcaag ctcaagaaac caccaaaggc
      301 cgatggcagt catcgggagg acgatgagga cagcaagccc aagccaaaca gcgacctgga
      361 agcagggaag agtttgcctt tcatctacgg ggacatcccc caaggcctgg ttgcagttcc
      421 cctggaggac tttgacccat actatttgac gcagaaaacc tttgtagtat taaacagagg
      481 gaaaactctc ttcagattta gtgccacgcc tgccttgtac attttaagtc cttttaacct
      541 gataagaaga atagctatta aaattttgat acattcagta tttagcatga tcattatgtg
      601 cactattttg accaactgtg tattcatgac ttttagtaac cctcctgact ggtcgaagaa
      661 tgtggagtac acgttcacag ggatttatac atttgaatca ctagtgaaaa tcattgcaag
      721 aggtttctgc atagatggct ttaccttttt acgggaccca tggaactggt tagatttcag
      781 tgtcatcatg atggcatatg tgacagagtt tgtggacctg ggcaatgtct cagcgctgag
      841 aacattcagg gttctccgag ctttgaaaac tatctctgta attccaggcc tgaagacaat
      901 tgtgggtgcc ctgattcagt ctgtgaagaa actgtcagat gtgatgatcc tgacagtgtt
      961 ctgcctgagt gtttttgcct tgatcggact gcagctgttc atggggaacc ttcgaaacaa
     1021 gtgtgttgtg tggcccataa acttcaacga gagctatctt gaaaatggca ccaaaggctt
     1081 tgattgggaa gagtatatca acaataaaac aaatttctac acagttcctg gcatgctgga
     1141 acctttactc tgtgggaaca gttctgatgc tgggcaatgc ccagagggat accagtgtat
     1201 gaaagcagga aggaacccca actatggtta cacaagtttt gacactttta gctgggcctt
     1261 cttggcatta tttcgcctta tgacccagga ctattgggaa aacttgtatc aattgacttt
     1321 acgagcagcc gggaaaacat acatgatctt cttcgtcttg gtcatctttg tgggttcttt
     1381 ctatctggtg aacttgatct tggctgtggt ggccatggct tatgaagaac agaatcaggc
     1441 aacactggag gaggcagaac aaaaagaggc tgaatttaaa gcaatgttgg agcaacttaa
     1501 gaagcaacag gaagaggcac aggctgctgc gatggccact tcagcaggaa ctgtctcaga
     1561 agatgccata gaggaagaag gtgaagaagg agggggctcc cctcggagct cttctgaaat
     1621 ctctaaactc agctcaaaga gtgcaaagga aagacgtaac aggagaaaga agaggaagca
     1681 aaaggaactc tctgaaggag aggagaaagg ggatcccgag aaggtgttta agtcagagtc
     1741 agaagatggc atgagaagga aggcctttcg gctgccagac aacagaatag ggaggaaatt
     1801 ttccatcatg aatcagtcac tgctcagcat cccaggctcg cccttcctct cccgccacaa
     1861 cagcaagagc agcatcttca gtttcagggg acctgggcgg ttccgagacc cgggctccga
     1921 gaatgagttc gcggatgacg agcacagcac ggtggaggag agcgagggcc gccgggactc
     1981 cctcttcatc cccatccggg cccgcgagcg ccggagcagc tacagcggct acagcggcta
     2041 cagccagggc agccgctcct cgcgcatctt ccccagcctg cggcgcagcg tgaagcgcaa
     2101 cagcacggtg gactgcaacg gcgtggtgtc cctcatcggc ggccccggct cccacatcgg
     2161 cgggcgtctc ctgccagagg ctacaactga ggtggaaatt aagaagaaag gccctggatc
     2221 tcttttagtt tccatggacc aattagcctc ctacgggcgg aaggacagaa tcaacagtat
     2281 aatgagtgtt gttacaaata cactagtaga agaactggaa gagtctcaga gaaagtgccc
     2341 gccatgctgg tataaatttg ccaacacttt cctcatctgg gagtgccacc cctactggat
     2401 aaaactgaaa gagattgtga acttgatagt tatggaccct tttgtggatt tagccatcac
     2461 catctgcatc gtcctgaata cactgtttat ggcaatggag caccatccta tgacaccaca
     2521 atttgaacat gtcttggctg taggaaatct ggttttcact ggaattttca cagcggaaat
     2581 gttcctgaag ctcatagcca tggatcccta ctattatttc caagaaggtt ggaacatttt
     2641 tgacggattt attgtctccc tcagtttaat ggaactgagt ctagcagacg tggaggggct
     2701 ttcagtgctg cgatctttcc gattgctccg agtcttcaaa ttggccaaat cctggcccac
     2761 cctgaacatg ctaatcaaga ttattggaaa ttcagtgggt gccctgggca acctgacact
     2821 ggtgctggcc attattgtct tcatctttgc cgtggtgggg atgcaactct ttggaaaaag
     2881 ctacaaagag tgtgtctgca agatcaacca ggactgtgaa ctccctcgct ggcatatgca
     2941 tgactttttc cattccttcc tcattgtctt tcgagtgttg tgcggggagt ggattgagac
     3001 catgtgggac tgcatggaag tggcaggcca ggccatgtgc ctcattgtct ttatgatggt
     3061 catggtgatt ggcaacttgg tggtgctgaa cctgtttctg gccttgctcc tgagctcctt
     3121 cagtgcagac aacctggctg ccacagatga cgatggggaa atgaacaacc tccagatctc
     3181 agtgatccgt atcaagaagg gtgtggcctg gaccaaacta aaggtgcacg ccttcatgca
     3241 ggcccacttt aagcagcgtg aggctgatga ggtgaagcct ctggatgagt tgtatgaaaa
     3301 gaaggccaac tgtatcgcca atcacaccgg tgcagacatc caccggaatg gtgacttcca
     3361 gaagaatggc aatggcacaa ccagcggcat tggcagcagc gtggagaagt acatcattga
     3421 tgaggaccac atgtccttca tcaacaaccc caacttgact gtacgggtac ccattgctgt
     3481 gggcgagtct gactttgaga acctcaacac agaggatgtt agcagcgagt cggatcctga
     3541 aggcagcaaa gataaactag atgacaccag ctcctctgaa ggaagcacca ttgatatcaa
     3601 accagaagta gaagaggtcc ctgtggaaca gcctgaggaa tacttggatc cagatgcctg
     3661 cttcacagaa ggttgtgtcc agcggttcaa gtgctgccag gtcaacatcg aggaagggct
     3721 aggcaagtct tggtggatcc tgcggaaaac ctgcttcctc atcgtggagc acaactggtt
     3781 tgagaccttc atcatcttca tgattctgct gagcagtggc gccctggcct tcgaggacat
     3841 ctacattgag cagagaaaga ccatccgcac catcctggaa tatgctgaca aagtcttcac
     3901 ctatatcttc atcctggaga tgttgctcaa gtggacagcc tatggcttcg tcaagttctt
     3961 caccaatgcc tggtgttggc tggacttcct cattgtggct gtctctttag tcagccttat
     4021 agctaatgcc ctgggctact cggaactagg tgccataaag tcccttagga ccctaagagc
     4081 tttgagaccc ttaagagcct tatcacgatt tgaagggatg agggtggtgg tgaatgcctt
     4141 ggtgggcgcc atcccctcca tcatgaatgt gctgctggtg tgtctcatct tctggctgat
     4201 tttcagcatc atgggagtta acttgtttgc gggaaagtac cactactgct ttaatgagac
     4261 ttctgaaatc cgatttgaaa ttgaagatgt caacaataaa actgaatgtg aaaagcttat
     4321 ggaggggaac aatacagaga tcagatggaa gaacgtgaag atcaactttg acaatgttgg
     4381 ggcaggatac ctggcccttc ttcaagtagc aaccttcaaa ggctggatgg acatcatgta
     4441 tgcagctgta gattcccgga agcctgatga gcagcctaag tatgaggaca atatctacat
     4501 gtacatctat tttgtcatct tcatcatctt cggctccttc ttcaccctga acctgttcat
     4561 tggtgtcatc attgataact tcaatcaaca aaagaaaaag ttcggaggtc aggacatctt
     4621 catgaccgaa gaacagaaga agtactacaa tgccatgaaa aagctgggct caaagaagcc
     4681 acagaaacct attccccgcc ccttgaacaa aatccaagga atcgtctttg attttgtcac
     4741 tcagcaagcc tttgacattg ttatcatgat gctcatctgc cttaacatgg tgacaatgat
     4801 ggtggagaca gacactcaaa gcaagcagat ggagaacatc ctctactgga ttaacctggt
     4861 gtttgttatc ttcttcacct gtgagtgtgt gctcaaaatg tttgcgttga ggcactacta
     4921 cttcaccatt ggctggaaca tcttcgactt cgtggtagtc atcctctcca ttgtgggaat
     4981 gttcctggca gatataattg agaaatactt tgtttcccca accctattcc gagtcatccg
     5041 attggcccgt attgggcgca tcttgcgtct gatcaaaggc gccaaaggga ttcgtaccct
     5101 gctctttgcc ttaatgatgt ccttgcctgc cctgttcaac atcggccttc tgctcttcct
     5161 ggtcatgttc atcttctcca tttttgggat gtccaatttt gcatatgtga agcacgaggc
     5221 tggtatcgat gacatgttca actttgagac atttggcaac agcatgatct gcctgtttca
     5281 aatcacaacc tcagctggtt gggatggcct gctgctgccc atcctaaacc gcccccctga
     5341 ctgcagccta gataaggaac acccagggag tggctttaag ggagattgtg ggaacccctc
     5401 agtgggcatc ttcttctttg taagctacat catcatctct ttcctaattg tcgtgaacat
     5461 gtacattgcc atcatcctgg agaacttcag tgtagccaca gaggaaagtg cagaccctct
     5521 gagtgaggat gactttgaga ccttctatga gatctgggag aagttcgacc ccgatgccac
     5581 ccagttcatt gagtactgta agctggcaga ctttgcagat gccttggagc atcctctccg
     5641 agtgcccaag cccaatacca ttgagctcat cgctatggat ctgccaatgg tgagcgggga
     5701 tcgcatccac tgcttggaca tcctttttgc cttcaccaag cgggtcctgg gagatagcgg
     5761 ggagttggac atcctgcggc agcagatgga agagcggttc gtggcatcca atccttccaa
     5821 agtgtcttac gagccaatca caaccacact gcgtcgcaag caggaggagg tatctgcagt
     5881 ggtcctgcag cgtgcctacc ggggacattt ggcaaggcgg ggcttcatct gcaaaaagac
     5941 aacttctaat aagctggaga atggaggcac acaccgggag aaaaaagaga gcaccccatc
     6001 tacagcctcc ctcccgtcct atgacagtgt aactaaacct gaaaaggaga aacagcagcg
     6061 ggcagaggaa ggaagaaggg aaagagccaa aagacaaaaa gaggtcagag aatccaagtg
     6121 ttagaggaga acaaaaattc agtattatac agatctaaaa ctcgcaagtg aaagattgtt
     6181 tacaaacttc ctgaatatta tcaatgcaga acagctgtgg agactctaac ctgaagatct
     6241 ataccaaacg tcgtctgctt accacgtaac acagctgcat cttgagcagt gacctgccaa
     6301 gggcaaagga ccccgctccc tagacttaca gattttctaa tgcttgggca ggtggttact
     6361 gcatgttcca catcagtcaa tgcaacttag gacaaaacta accagataca gaaacagaag
     6421 agaggctgcc gggaccagca tatttccgtt gcagccaaat ggattttatt ttttcatttt
     6481 attgattctc agaagcagaa agcatcactt taaaagttcg tttgttcatg caaactatat
     6541 ttgcattctt acattagtta agctaagcag caaaaagaaa acacacacac acactcacat
     6601 ttagcccatg tcatttaatt gtcagtttct ttgacataaa gcgcatcttc tccacatggg
     6661 cttcacgtgg tttggagatg ggtgggggaa aacaatcagg tttcttcagg ctgaggagga
     6721 cttgctcagg ccgattccaa acattgtgct cgttcaatgc gtagaaatga tttgcatgat
     6781 ggcatgccgt gatcagaagt catgcatgag atccatacac cacaggacac tactaatcta
     6841 gtcccttgca ctgggtcagc ctttggacag gacccagccc tgcaccgttc actgtatttg
     6901 gagaaaatgg taagagttcc ataccggcta caattctttg agttcttaaa agtccttcat
     6961 acaccttctg ggtagggaaa caaccaacta attgactaac accaccaaca acaaaaaaca
     7021 aacccaatcc aacaagcaga tggatccgtt gcgtgtatat gtttaacaga catctctaac
     7081 atacagccat tgttgcacat tttgcaagat gaactattta atgctgctct gtgtccagta
     7141 catgggggag actttgatcc caaatggctt gtactattta tgtcactgta aaaccaaatc
     7201 ctagggctaa aaaaaaaatt catttgtatc ttgcaatatt tatgacgatc gtttagcctt
     7261 catactggag aattgacact tatttggggt agagataaaa gtgcttttca aagtagcacc
     7321 agttatctct aggggtaatt tggggaactt aaaatgacct ttcattggga gttatggggt
     7381 gctcatttca tttcgtatca tgtcctctgc attgtggctt tctccaggca tgggtcgata
     7441 ccgcgagggg ttcagatatt ctgaactgac ctctctcttc tgcagagggg tcatcgcata
     7501 ctcacacact gcatggctcc ccctgcctcc atcacatttc tccactgagc agggcttccc
     7561 ttgcccacag aggtgggtga gggtagcagc attggggtta cggctgtggt ttctttcact
     7621 tattattaga ataatagtca ctggtgagac ctgaggtaga gatggagccg cctctgaacc
     7681 aagcccactt ggtttcttta ctgcactggt tttcatgaga aagcaattta atattaattc
     7741 tttaggatgt tccagcttcc ctcagtgcat tcccttgcag ccagttttgc tatctgctta
     7801 gcgacctgcc cattgtcatt gaagtgtggc ttctggggga gcgtcatatc ttccattcca
     7861 tctcctgctt taggaaagac agtttttagt ccaggggttt ctcacctaat gactggattt
     7921 caaagccaag atgctgcagt tgaatgtgtc aggaagtctc tatacaaaga gtgaaaagag
     7981 actttagcat gtgaaccaaa tccaaataaa ttcttcctga accatacact agagggggaa
     8041 gaagaaccaa cccataatca gacacaccga tctgccttgc agtagaagca ctttgaaggt
     8101 aatttcacag tccaccagac tagttttgca tagaacaaac cacagtaagt cggcaagcag
     8161 agctttctat cttgttggac cactagaaac tgtccagctc tcttaagcct gcttctgcag
     8221 cagcatccgt agaatttttg tactacaccc aataccagac tgggaaggcc tatctttcag
     8281 tgaccctgct aactgctagg atgaatgtat agtaacatgt acaggctaca ttgtaaacag
     8341 cacaaaacag aagctgacat gtgtggttat tctttttaag agaattataa atactgtaat
     8401 atataatttt attttattgg catgcctttt tgtaaataca aattattaac ttattaaata
     8461 ggaaatggct tctggcttat gccattgtca tgtttatttt tattttttat acttttcttt
     8521 cttcttagaa cttagatgct gtcagccaat gtacatcccc aaaccagaca gacagacaaa
     8581 aaatttttta ttgctaatgg tcttttccaa aacaacaaaa aatatatatt tttgttccaa
     8641 tgtgcaataa attctaacca cgtctatgca agatttggct aaacttaata attgttctta
     8701 ggtcttgaga tgggcaacag agctgtaaga tcccgccaag tacaccatgt gctcatgcta
     8761 gtgatgtcat taagctacta gggcatagtg atgagatttt tctgagatct aaccgtttcc
     8821 ctccgctagc tcctgacccc agcccagcag gctgtctcct cagtaacaca tgggccttgg
     8881 tatctggcac ccatcagcca gcctcatagg aaagaagcca cctccgctgt atttgaagtt
     8941 tcacttttta aaaaaaaaat gtttcccacc tttttatcct tcacccactg tccttccacc
     9001 tgctcactca ctcactctct cacccatcct gctccacact tctttggtag taaatgacac
     9061 catcaccagc agtttacacc cgcagttaac aggcatcgaa ctgaaagaat cagtgatgac
     9121 gtttttgtgc atcttcgctg agtgggtaag tggcaatgct ttgccaaata ttaacgaagc
     9181 caatcaaaga gcagcgcagc cacagtatca ctgcacatat atatatagat atataaatat
     9241 aatataaata tttatttttt gtagccaggt ccttggtgca gtatttatac tccacaatcc
     9301 accttttaaa aaaaggacaa aaagcaaaaa gatgtgtgat gctgttaata atttaaaatg
     9361 cagagccaaa gccactgagc agcagctgac atggttgctg gtttgtgtgt gaaaaacatt
     9421 ttccccctct tctttcctta acctccttgt tgcttaggat gaggagactc tgtaatttaa
     9481 agcagaggac gagaggagga agaaacttcc atgttaacat ggcctactct cactcctttc
     9541 taaacgagcc acttttggcg tcagaagttg actggagaga gataaacaaa ctcccagtca
     9601 aagcccctaa agcgacagtg cccagagttc ttttattttt tgctgcaacc aacgtaaacc
     9661 tgtaaaagac caacagtgaa gattagtgta ttagtgaatg catggaggcc aatgctgccc
     9721 taatgagacg tgataaaaag tccatcactc ctctaagcca aaaggaaaca aaataaaaat
     9781 gacccttttt actgtacaag ggaagcctgt cttggtgtgt ttgttgcttg actatcccaa
     9841 ttcttgagtc ctgggagggg tctgggaatt gggaccttag cacatttggg gaaaggaggg
     9901 gaatagaggg aagagaaatc aaaggggctc tcgcaaagtt ctggctaaga aggagagatt
     9961 ggaagaaaag caaggtcctg acccccccca gatccccact cacccattgc atgtggcatt
    10021 ccccactact ggccggtgct tagaggccca taaaacctct cttcacctaa tccacctggc
    10081 agagccctca gagatgacgt cagccaaccc agctggactg gccttacttc tgcctttttc
    10141 acctgtatgt taaaaaacaa aaacaaaaaa accctatccc tgtgccccaa agctagggca
    10201 tgtgcgatgc ccaacagcct ccactctgca agctcatact gggccctgcc ctccatcgcc
    10261 ccagacaggc ccacagacga gggctggtct ccttcacatt tgtgtggggg tgggggcacg
    10321 cttcctcttc cttctctccc tgtccccctt tggggacatc cctctctagg cctaggccat
    10381 gtctcctggt tcaggaacac tctctcagga ggatctcgaa gtgcccactc tagtccgctc
    10441 cagtagctcc actctggagc gagatggctg gtaggtcagt ccccatcctc ctcccctcgg
    10501 cccccagcct ccaggcctgc tgtggaggtg agatcgccat gggtcatgac tttgtgactt
    10561 gagtcctggg catcttctgt aatgatgcct ctttcttttc tcagatgttg cccacagttt
    10621 agcaaaaagc ttcgttcatt ttcaggtact ccccaccccc taaagaattg gctgcaactt
    10681 gccagccagg gggacatcag gagagaagag gaagtctggg ttgggagggg cccaagtttt
    10741 gcaacagagt ttccattgtt cagctctgtg ctttcttgga ttccctttcc aagattacca
    10801 agatttcctc cattaaaact cctgaggcac cccctgcccc tgccccccac tgctgtttgc
    10861 tgtacataga ggctaagtgg ggtggggtgg gtgggtgtgc acatgtggtg tgtgtgtgtg
    10921 tgtgtgaaga atgtatatag gtaaagggga gtgtggtcca gtggttccag aaaagggaca
    10981 gaactgtgtt ctgtccctag cttgaccgct ggctcgctgt gtggccttgg gcaagtcact
    11041 taacctctct gtgcctcagt ttccccatct gtaaaatggg gataataata ctgacctacc
    11101 tcacaggggt gttgtgaggc tttaactaaa tgttttgtaa agcgttttga gatacagagg
    11161 caaaggcgct agggaagggc aaagtattat tggacttaag gaagctgaaa tggtaccata
    11221 aatgctgcta ttctgagagc cattttaaac tgcactgctc caaccacccc cgcttctcat
    11281 caccaggaca gcaggtcgag aaaatgtctt tcctttcact tgcttctggg gtttgtgatt
    11341 attcaagaca attttttccc cttgctgtat ctccttccct cacctcctcg acttgttccc
    11401 tgttctgttt atgcggaaat ggcagaaatg cttgagaaat gagaatgtgt aagtggattg
    11461 gaagtttata gtatgaaatt tcaattttac tttgtactgt acatcttttt actttagaat
    11521 ttgcaataaa gggttacgtc aatcttgttt tcaactctc
//
