LOCUS       NM_002976               7465 bp    mRNA    linear   PRI 18-NOV-2024
DEFINITION  Homo sapiens sodium voltage-gated channel alpha subunit 7 (SCN7A),
            transcript variant 1, mRNA.
ACCESSION   NM_002976 XM_006712681
VERSION     NM_002976.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7465)
  AUTHORS   Yuan,P., Rao,W., Lin,Z., Liu,S., Lin,X., Wu,C., Lin,X., Hu,Z. and
            Ye,W.
  TITLE     Genomic analyses reveal SCN7A is associated with the prognosis of
            esophageal squamous cell carcinoma
  JOURNAL   Esophagus 19 (2), 303-315 (2022)
   PUBMED   34993672
  REMARK    GeneRIF: Genomic analyses reveal SCN7A is associated with the
            prognosis of esophageal squamous cell carcinoma.
REFERENCE   2  (bases 1 to 7465)
  AUTHORS   Noland,C.L., Chua,H.C., Kschonsak,M., Heusser,S.A., Braun,N.,
            Chang,T., Tam,C., Tang,J., Arthur,C.P., Ciferri,C., Pless,S.A. and
            Payandeh,J.
  TITLE     Structure-guided unlocking of NaX reveals a non-selective
            tetrodotoxin-sensitive cation channel
  JOURNAL   Nat Commun 13 (1), 1416 (2022)
   PUBMED   35301303
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 7465)
  AUTHORS   Li,W., Zhou,K., Li,M., Hu,Q., Wei,W., Liu,L. and Zhao,Q.
  TITLE     Identification of SCN7A as the key gene associated with tumor
            mutation burden in gastric cancer
  JOURNAL   BMC Gastroenterol 22 (1), 45 (2022)
   PUBMED   35123417
  REMARK    GeneRIF: Identification of SCN7A as the key gene associated with
            tumor mutation burden in gastric cancer.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 7465)
  AUTHORS   Xu,W., Hong,S.J., Zhong,A., Xie,P., Jia,S., Xie,Z., Zeitchek,M.,
            Niknam-Bienia,S., Zhao,J., Porterfield,D.M., Surmeier,D.J.,
            Leung,K.P., Galiano,R.D. and Mustoe,T.A.
  TITLE     Sodium channel Nax is a regulator in epithelial sodium homeostasis
  JOURNAL   Sci Transl Med 7 (312), 312ra177 (2015)
   PUBMED   26537257
  REMARK    GeneRIF: The authors demonstrated that the sodium channel Nax
            functions as a sodium sensor.  With increased extracellular sodium,
            Nax up-regulates prostasin, which results in activation of the
            sodium channel ENaC.
REFERENCE   5  (bases 1 to 7465)
  AUTHORS   Goldin,A.L., Barchi,R.L., Caldwell,J.H., Hofmann,F., Howe,J.R.,
            Hunter,J.C., Kallen,R.G., Mandel,G., Meisler,M.H., Netter,Y.B.,
            Noda,M., Tamkun,M.M., Waxman,S.G., Wood,J.N. and Catterall,W.A.
  TITLE     Nomenclature of voltage-gated sodium channels
  JOURNAL   Neuron 28 (2), 365-368 (2000)
   PUBMED   11144347
REFERENCE   6  (bases 1 to 7465)
  AUTHORS   Plummer,N.W. and Meisler,M.H.
  TITLE     Evolution and diversity of mammalian sodium channel genes
  JOURNAL   Genomics 57 (2), 323-331 (1999)
   PUBMED   10198179
  REMARK    Review article
REFERENCE   7  (bases 1 to 7465)
  AUTHORS   George,A.L. Jr., Knops,J.F., Han,J., Finley,W.H., Knittle,T.J.,
            Tamkun,M.M. and Brown,G.B.
  TITLE     Assignment of a human voltage-dependent sodium channel
            alpha-subunit gene (SCN6A) to 2q21-q23
  JOURNAL   Genomics 19 (2), 395-397 (1994)
   PUBMED   8188276
REFERENCE   8  (bases 1 to 7465)
  AUTHORS   Boyle,M.B. and Heslip,L.A.
  TITLE     Voltage-dependent Na+ channel mRNA expression in pregnant
            myometrium
  JOURNAL   Recept Channels 2 (3), 249-253 (1994)
   PUBMED   7874451
REFERENCE   9  (bases 1 to 7465)
  AUTHORS   George,A.L. Jr., Knittle,T.J. and Tamkun,M.M.
  TITLE     Molecular cloning of an atypical voltage-gated sodium channel
            expressed in human heart and uterus: evidence for a distinct gene
            family
  JOURNAL   Proc Natl Acad Sci U S A 89 (11), 4893-4897 (1992)
   PUBMED   1317577
REFERENCE   10 (bases 1 to 7465)
  AUTHORS   Han,J.A., Lu,C.M., Brown,G.B. and Rado,T.A.
  TITLE     Direct amplification of a single dissected chromosomal segment by
            polymerase chain reaction: a human brain sodium channel gene is on
            chromosome 2q22-q23
  JOURNAL   Proc Natl Acad Sci U S A 88 (2), 335-339 (1991)
   PUBMED   1846440
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC092583.2 and AC074101.5.
            This sequence is a reference standard in the RefSeqGene project.
            
            On or before Jan 8, 2020 this sequence version replaced
            XM_006712681.3, NM_002976.3.
            
            Summary: This gene encodes one of the many voltage-gated sodium
            channel proteins. For proper functioning of neurons and muscles
            during action potentials, voltage-gated sodium channels direct
            sodium ion diffusion for membrane depolarization. This sodium
            channel protein has some atypical characteristics; the similarity
            between the human and mouse proteins is lower compared to other
            orthologous sodium channel pairs. Also, the S4 segments, which
            sense voltage changes, have fewer positive charged residues that in
            other sodium channels; domain 4 has fewer arginine and lysine
            residues compared to other sodium channel proteins. Several
            alternatively spliced transcript variants exist, but the
            full-length natures of all of them remain unknown. [provided by
            RefSeq, Dec 2011].
            
            Transcript Variant: This variant (1) is the protein-coding
            transcript.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMEA1965299, SAMEA1966682
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            MANE Ensembl match        :: ENST00000643258.1/ ENSP00000496114.1
            RefSeq Select criteria    :: based on manual assertion,
                                         conservation, expression, longest
                                         protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-282               AC092583.2         8088-8369           c
            283-395             AC092583.2         976-1088            c
            396-643             AC074101.5         143306-143553       c
            644-762             AC074101.5         140069-140187       c
            763-852             AC074101.5         139642-139731       c
            853-981             AC074101.5         138160-138288       c
            982-1073            AC074101.5         136458-136549       c
            1074-1280           AC074101.5         131624-131830       c
            1281-1350           AC074101.5         131305-131374       c
            1351-1492           AC074101.5         128232-128373       c
            1493-1699           AC074101.5         122713-122919       c
            1700-1796           AC074101.5         113455-113551       c
            1797-2035           AC074101.5         110605-110843       c
            2036-2209           AC074101.5         109346-109519       c
            2210-2566           AC074101.5         107239-107595       c
            2567-3001           AC074101.5         98161-98595         c
            3002-3107           AC074101.5         95012-95117         c
            3108-3262           AC074101.5         93631-93785         c
            3263-3436           AC074101.5         89102-89275         c
            3437-3544           AC074101.5         87033-87140         c
            3545-3823           AC074101.5         82550-82828         c
            3824-3877           AC074101.5         78911-78964         c
            3878-4015           AC074101.5         78373-78510         c
            4016-4120           AC074101.5         76063-76167         c
            4121-4391           AC074101.5         75508-75778         c
            4392-7465           AC074101.5         69416-72489         c
FEATURES             Location/Qualifiers
     source          1..7465
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q24.3"
     gene            1..7465
                     /gene="SCN7A"
                     /gene_synonym="NaG; Nav2.1; Nav2.2; SCN6A"
                     /note="sodium voltage-gated channel alpha subunit 7"
                     /db_xref="GeneID:6332"
                     /db_xref="HGNC:HGNC:10594"
                     /db_xref="MIM:182392"
     exon            1..282
                     /gene="SCN7A"
                     /gene_synonym="NaG; Nav2.1; Nav2.2; SCN6A"
                     /inference="alignment:Splign:2.1.0"
     exon            283..395
                     /gene="SCN7A"
                     /gene_synonym="NaG; Nav2.1; Nav2.2; SCN6A"
                     /inference="alignment:Splign:2.1.0"
     exon            396..643
                     /gene="SCN7A"
                     /gene_synonym="NaG; Nav2.1; Nav2.2; SCN6A"
                     /inference="alignment:Splign:2.1.0"
     CDS             410..5458
                     /gene="SCN7A"
                     /gene_synonym="NaG; Nav2.1; Nav2.2; SCN6A"
                     /note="sodium channel, voltage-gated, type VI, alpha
                     polypeptide; voltage-dependent sodium channel alpha
                     subunit; sodium channel protein type VII subunit alpha;
                     nax channel; putative voltage-gated sodium channel subunit
                     alpha Nax; sodium channel protein cardiac and skeletal
                     muscle subunit alpha; sodium channel, voltage-gated, type
                     VII, alpha subunit; atypical sodium channel Nav2.1"
                     /codon_start=1
                     /product="sodium channel protein type 7 subunit alpha"
                     /protein_id="NP_002967.2"
                     /db_xref="CCDS:CCDS46442.1"
                     /db_xref="GeneID:6332"
                     /db_xref="HGNC:HGNC:10594"
                     /db_xref="MIM:182392"
                     /translation="MLASPEPKGLVPFTKESFELIKQHIAKTHNEDHEEEDLKPTPDL
                     EVGKKLPFIYGNLSQGMVSEPLEDVDPYYYKKKNTFIVLNKNRTIFRFNAASILCTLS
                     PFNCIRRTTIKVLVHPFFQLFILISVLIDCVFMSLTNLPKWRPVLENTLLGIYTFEIL
                     VKLFARGVWAGSFSFLGDPWNWLDFSVTVFEVIIRYSPLDFIPTLQTARTLRILKIIP
                     LNQGLKSLVGVLIHCLKQLIGVIILTLFFLSIFSLIGMGLFMGNLKHKCFRWPQENEN
                     ETLHNRTGNPYYIRETENFYYLEGERYALLCGNRTDAGQCPEGYVCVKAGINPDQGFT
                     NFDSFGWALFALFRLMAQDYPEVLYHQILYASGKVYMIFFVVVSFLFSFYMASLFLGI
                     LAMAYEEEKQRVGEISKKIEPKFQQTGKELQEGNETDEAKTIQIEMKKRSPISTDTSL
                     DVLEDATLRHKEELEKSKKICPLYWYKFAKTFLIWNCSPCWLKLKEFVHRIIMAPFTD
                     LFLIICIILNVCFLTLEHYPMSKQTNTLLNIGNLVFIGIFTAEMIFKIIAMHPYGYFQ
                     VGWNIFDSMIVFHGLIELCLANVAGMALLRLFRMLRIFKLGKYWPTFQILMWSLSNSW
                     VALKDLVLLLFTFIFFSAAFGMKLFGKNYEEFVCHIDKDCQLPRWHMHDFFHSFLNVF
                     RILCGEWVETLWDCMEVAGQSWCIPFYLMVILIGNLLVLYLFLALVSSFSSCKDVTAE
                     ENNEAKNLQLAVARIKKGINYVLLKILCKTQNVPKDTMDHVNEVYVKEDISDHTLSEL
                     SNTQDFLKDKEKSSGTEKNATENESQSLIPSPSVSETVPIASGESDIENLDNKEIQSK
                     SGDGGSKEKIKQSSSSECSTVDIAISEEEEMFYGGERSKHLKNGCRRGSSLGQISGAS
                     KKGKIWQNIRKTCCKIVENNWFKCFIGLVTLLSTGTLAFEDIYMDQRKTIKILLEYAD
                     MIFTYIFILEMLLKWMAYGFKAYFSNGWYRLDFVVVIVFCLSLIGKTREELKPLISMK
                     FLRPLRVLSQFERMKVVVRALIKTTLPTLNVFLVCLMIWLIFSIMGVDLFAGRFYECI
                     DPTSGERFPSSEVMNKSRCESLLFNESMLWENAKMNFDNVGNGFLSLLQVATFNGWIT
                     IMNSAIDSVAVNIQPHFEVNIYMYCYFINFIIFGVFLPLSMLITVIIDNFNKHKIKLG
                     GSNIFITVKQRKQYRRLKKLMYEDSQRPVPRPLNKLQGFIFDVVTSQAFNVIVMVLIC
                     FQAIAMMIDTDVQSLQMSIALYWINSIFVMLYTMECILKLIAFRCFYFTIAWNIFDFM
                     VVIFSITGLCLPMTVGSYLVPPSLVQLILLSRIIHMLRLGKGPKVFHNLMLPLMLSLP
                     ALLNIILLIFLVMFIYAVFGMYNFAYVKKEAGINDVSNFETFGNSMLCLFQVAIFAGW
                     DGMLDAIFNSKWSDCDPDKINPGTQVRGDCGNPSVGIFYFVSYILISWLIIVNMYIVV
                     VMEFLNIASKKKNKTLSEDDFRKFFQVWKRFDPDRTQYIDSSKLSDFAAALDPPLFMA
                     KPNKGQLIALDLPMAVGDRIHCLDILLAFTKRVMGQDVRMEKVVSEIESGFLLANPFK
                     ITCEPITTTLKRKQEAVSATIIQRAYKNYRLRRNDKNTSDIHMIDGDRDVHATKEGAY
                     FDKAKEKSPIQSQI"
     exon            644..762
                     /gene="SCN7A"
                     /gene_synonym="NaG; Nav2.1; Nav2.2; SCN6A"
                     /inference="alignment:Splign:2.1.0"
     exon            763..852
                     /gene="SCN7A"
                     /gene_synonym="NaG; Nav2.1; Nav2.2; SCN6A"
                     /inference="alignment:Splign:2.1.0"
     exon            853..981
                     /gene="SCN7A"
                     /gene_synonym="NaG; Nav2.1; Nav2.2; SCN6A"
                     /inference="alignment:Splign:2.1.0"
     exon            982..1073
                     /gene="SCN7A"
                     /gene_synonym="NaG; Nav2.1; Nav2.2; SCN6A"
                     /inference="alignment:Splign:2.1.0"
     exon            1074..1280
                     /gene="SCN7A"
                     /gene_synonym="NaG; Nav2.1; Nav2.2; SCN6A"
                     /inference="alignment:Splign:2.1.0"
     exon            1281..1350
                     /gene="SCN7A"
                     /gene_synonym="NaG; Nav2.1; Nav2.2; SCN6A"
                     /inference="alignment:Splign:2.1.0"
     exon            1351..1492
                     /gene="SCN7A"
                     /gene_synonym="NaG; Nav2.1; Nav2.2; SCN6A"
                     /inference="alignment:Splign:2.1.0"
     exon            1493..1699
                     /gene="SCN7A"
                     /gene_synonym="NaG; Nav2.1; Nav2.2; SCN6A"
                     /inference="alignment:Splign:2.1.0"
     exon            1700..1796
                     /gene="SCN7A"
                     /gene_synonym="NaG; Nav2.1; Nav2.2; SCN6A"
                     /inference="alignment:Splign:2.1.0"
     exon            1797..2035
                     /gene="SCN7A"
                     /gene_synonym="NaG; Nav2.1; Nav2.2; SCN6A"
                     /inference="alignment:Splign:2.1.0"
     exon            2036..2209
                     /gene="SCN7A"
                     /gene_synonym="NaG; Nav2.1; Nav2.2; SCN6A"
                     /inference="alignment:Splign:2.1.0"
     exon            2210..2566
                     /gene="SCN7A"
                     /gene_synonym="NaG; Nav2.1; Nav2.2; SCN6A"
                     /inference="alignment:Splign:2.1.0"
     exon            2567..3001
                     /gene="SCN7A"
                     /gene_synonym="NaG; Nav2.1; Nav2.2; SCN6A"
                     /inference="alignment:Splign:2.1.0"
     exon            3002..3107
                     /gene="SCN7A"
                     /gene_synonym="NaG; Nav2.1; Nav2.2; SCN6A"
                     /inference="alignment:Splign:2.1.0"
     exon            3108..3262
                     /gene="SCN7A"
                     /gene_synonym="NaG; Nav2.1; Nav2.2; SCN6A"
                     /inference="alignment:Splign:2.1.0"
     exon            3263..3436
                     /gene="SCN7A"
                     /gene_synonym="NaG; Nav2.1; Nav2.2; SCN6A"
                     /inference="alignment:Splign:2.1.0"
     exon            3437..3544
                     /gene="SCN7A"
                     /gene_synonym="NaG; Nav2.1; Nav2.2; SCN6A"
                     /inference="alignment:Splign:2.1.0"
     exon            3545..3823
                     /gene="SCN7A"
                     /gene_synonym="NaG; Nav2.1; Nav2.2; SCN6A"
                     /inference="alignment:Splign:2.1.0"
     exon            3824..3877
                     /gene="SCN7A"
                     /gene_synonym="NaG; Nav2.1; Nav2.2; SCN6A"
                     /inference="alignment:Splign:2.1.0"
     exon            3878..4015
                     /gene="SCN7A"
                     /gene_synonym="NaG; Nav2.1; Nav2.2; SCN6A"
                     /inference="alignment:Splign:2.1.0"
     exon            4016..4120
                     /gene="SCN7A"
                     /gene_synonym="NaG; Nav2.1; Nav2.2; SCN6A"
                     /inference="alignment:Splign:2.1.0"
     exon            4121..4391
                     /gene="SCN7A"
                     /gene_synonym="NaG; Nav2.1; Nav2.2; SCN6A"
                     /inference="alignment:Splign:2.1.0"
     exon            4392..7465
                     /gene="SCN7A"
                     /gene_synonym="NaG; Nav2.1; Nav2.2; SCN6A"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7442..7447
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SCN7A"
                     /gene_synonym="NaG; Nav2.1; Nav2.2; SCN6A"
                     /note="hexamer: AATAAA"
     polyA_site      7465
                     /gene="SCN7A"
                     /gene_synonym="NaG; Nav2.1; Nav2.2; SCN6A"
                     /note="major polyA site"
ORIGIN      
        1 ggctccactg gacatctggg taggaggggt ctctgggtca gtggctggag agtcgcccca
       61 gtgagtcggt gtctaactgg ccatttagtg gggcccattt actgcagagc cacagctggt
      121 gaggacagac gccagcctaa cagcagcttc tgtacaacta gagctcagaa aacccgagag
      181 atccgtggag aaagagaagc ccaggaggag gaaagggctc caaggagccc tgctcctgaa
      241 gggtccctca ggcactggtg gagacctgtc gcttcgtagc aagtcctcca ccatgtgaat
      301 gccaacatgg ccaggtcatt agagctgagg gaaaactagt gcccaaagat atgaaaagag
      361 tgtggatctt ctggagaagt gctgttgttc aacaggtaca aaattggaaa tgttggcttc
      421 accagaacct aagggccttg ttcccttcac taaagagtct tttgaactta taaaacagca
      481 tattgctaaa acacataatg aagaccatga agaagaagac ttaaagccaa ctcctgattt
      541 ggaagttggc aaaaagcttc catttattta tggaaacctt tctcaaggaa tggtgtcaga
      601 gcccttggaa gatgtggacc catattacta caagaaaaaa aatactttca tagtattaaa
      661 taaaaataga acaatcttca gattcaatgc ggcttccatc ttgtgtacat tgtctccttt
      721 caattgtatt agaagaacaa ctatcaaggt tttggtacat ccctttttcc aactgtttat
      781 tctaattagt gtcctgattg attgcgtatt catgtccctg actaatttgc caaaatggag
      841 accagtatta gagaatactt tgcttggaat ttacacattt gaaatacttg taaaactctt
      901 tgcaagaggt gtctgggcag gatcattttc cttcctcggt gatccatgga actggctcga
      961 tttcagcgta actgtgtttg aggttattat aagatactca cctctggact tcattccaac
     1021 gcttcaaact gcaagaactt tgagaatttt aaaaattatt cctttaaatc aaggtctgaa
     1081 atcccttgta ggggtcctga tccactgctt gaagcagctt attggtgtca ttatcctaac
     1141 tctgtttttt ctgagcatat tttctctaat tgggatgggg ctcttcatgg gcaacttgaa
     1201 acataaatgt tttcgatggc cccaagagaa tgaaaatgaa accctgcaca acagaactgg
     1261 aaacccatat tatattcgag aaacagaaaa cttttattat ttggaaggag aaagatatgc
     1321 tctcctttgt ggcaacagga cagatgctgg tcagtgtcct gaaggatatg tgtgtgtaaa
     1381 agctggcata aatcctgatc aaggcttcac aaattttgac agttttggct gggccttatt
     1441 tgccctattt cggttaatgg ctcaggatta ccctgaagta ctttatcacc agatacttta
     1501 tgcttctggg aaggtctaca tgatattttt tgtggtggta agttttttgt tttcctttta
     1561 tatggcaagt ttgttcttag gcatacttgc catggcctat gaagaagaaa agcagagagt
     1621 tggtgaaata tctaagaaga ttgaaccaaa atttcaacag actggaaaag aacttcaaga
     1681 aggaaatgaa acagatgagg ccaagaccat acaaatagaa atgaagaaaa ggtcaccaat
     1741 ttccacagac acatcattgg atgtgttgga agatgctact ctcagacata aggaagaact
     1801 tgaaaaatcc aagaagatat gcccattata ctggtataag tttgctaaaa ctttcttgat
     1861 ctggaattgt tctccctgtt ggttaaaatt gaaagagttt gtccatagga ttataatggc
     1921 accatttact gatcttttcc ttatcatatg cataatttta aacgtatgtt ttctgacctt
     1981 ggagcattat ccaatgagta aacaaactaa cactcttctc aacattggaa acctggtttt
     2041 cattggaatt ttcacagcag aaatgatttt taaaataatt gcaatgcatc catatgggta
     2101 tttccaagta ggttggaaca tttttgatag catgatagtg ttccatggtt taatagaact
     2161 ttgtctagca aatgttgcag gaatggctct tcttcgatta ttcaggatgt taagaatttt
     2221 caagttggga aagtattggc caacattcca gattttgatg tggtctctta gtaactcatg
     2281 ggtggccctg aaagacttgg tcctgttgtt gttcacattc atcttctttt ctgctgcatt
     2341 cggcatgaag ctgtttggta agaattatga agaatttgtc tgccacatag acaaagactg
     2401 tcaactccca cgctggcaca tgcatgactt tttccactcc ttcctgaatg tgttccgaat
     2461 tctctgtgga gagtgggtag agaccttgtg ggactgtatg gaggttgcag gccaatcctg
     2521 gtgtattcct ttttacctga tggtcatttt aattggaaat ttactggtac tttacctgtt
     2581 tctggcattg gtgagctcat ttagttcatg caaggatgta acagctgaag agaataatga
     2641 agcaaaaaat ctccagcttg cagtggcaag aattaaaaaa ggaataaact atgtgcttct
     2701 taaaatacta tgcaaaacac aaaatgtccc aaaggacaca atggaccatg taaatgaggt
     2761 atatgttaaa gaagatattt ctgaccatac cctttctgaa ttgagcaaca cccaagattt
     2821 tctcaaagat aaggaaaaaa gcagtggcac agagaaaaac gctactgaaa atgagagcca
     2881 atcacttatc cccagtccta gtgtctcaga aactgtacca attgcttcag gagaatctga
     2941 tatagaaaat ctggataata aggagattca gagtaagtct ggtgatggag gcagcaaaga
     3001 gaaaataaag caatctagct catctgaatg cagtactgtt gatattgcta tctctgaaga
     3061 agaagaaatg ttctatggag gtgaaagatc aaagcatctg aaaaatggtt gcagacgcgg
     3121 atcttcactt ggtcaaatca gtggagcatc caagaaagga aaaatctggc agaacatcag
     3181 gaaaacctgc tgcaagattg tagagaacaa ttggtttaag tgttttattg ggcttgttac
     3241 tctgctcagc actggcactc tggcttttga agatatatat atggatcaga gaaagacaat
     3301 taaaatttta ttagaatatg ctgacatgat ctttacttat atcttcattc tggaaatgct
     3361 tctaaaatgg atggcatatg gttttaaggc ctatttctct aatggctggt acaggctgga
     3421 cttcgtggtt gttattgtgt tttgtcttag cttaataggc aaaactcggg aagaactaaa
     3481 acctcttatt tccatgaaat tccttcggcc cctcagagtt ctatctcaat ttgaaagaat
     3541 gaaggtggtt gtgagagctt tgatcaaaac aaccttaccc actttgaatg tgtttcttgt
     3601 ctgcctgatg atctggctga tttttagtat catgggagta gacttatttg ctggcagatt
     3661 ctatgaatgc attgacccaa caagtggaga aaggtttcct tcatctgaag tcatgaataa
     3721 gagtcggtgt gaaagccttc tgtttaacga atccatgcta tgggaaaatg caaaaatgaa
     3781 ctttgataat gttggaaatg gtttcctttc tctgcttcaa gtagcaacat ttaatggatg
     3841 gatcactatt atgaattcag caattgattc tgttgctgtt aatatacagc ctcattttga
     3901 agtcaacatc tacatgtatt gttactttat caactttatt atatttggag tatttctccc
     3961 tctgagtatg ctgattactg ttattattga taatttcaac aagcataaaa taaagctggg
     4021 aggctcaaat atctttataa cggttaaaca gagaaaacag taccgcaggc tgaagaagct
     4081 aatgtatgag gattctcaaa gaccagtacc tcgcccatta aacaagctcc aaggattcat
     4141 ctttgatgtg gtaacaagcc aagcttttaa tgtcattgtt atggttctta tatgtttcca
     4201 agcaatagcc atgatgatag acactgatgt tcagagtcta caaatgtcca ttgctctcta
     4261 ctggattaac tcaatttttg ttatgctata tactatggaa tgtatactga agctcatcgc
     4321 tttccgttgt ttttatttca ccattgcgtg gaacattttt gattttatgg tggttatttt
     4381 ctccatcaca ggactatgtc tgcctatgac agtaggatcc taccttgtgc ctccttcact
     4441 tgtgcaactg atacttctct cacggatcat tcacatgctg cgtcttggaa aaggaccaaa
     4501 ggtgtttcat aatctgatgc ttcctttgat gctgtccctc ccagcattat tgaacatcat
     4561 tcttctcatc ttcctggtca tgttcatcta tgccgtattt ggaatgtata attttgccta
     4621 tgttaaaaaa gaagctggaa ttaatgatgt gtctaatttt gaaacctttg gcaacagtat
     4681 gctctgtctt tttcaagttg caatatttgc tggttgggat gggatgcttg atgcaatttt
     4741 caacagtaaa tggtctgact gtgatcctga taaaattaac cctgggactc aagttagagg
     4801 agattgtggg aacccctctg ttgggatttt ttattttgtc agttatatcc tcatatcatg
     4861 gctgatcatt gtaaatatgt acattgttgt tgtcatggag tttttaaata ttgcttctaa
     4921 gaagaaaaac aagaccttga gtgaagatga ttttaggaaa ttctttcagg tatggaaaag
     4981 gtttgatcct gataggaccc agtacataga ctctagcaag ctttcagatt ttgcagctgc
     5041 tcttgatcct cctcttttca tggcaaaacc aaacaagggc cagctcattg ctttggacct
     5101 ccccatggct gttggggaca gaattcattg cctcgatatc ttacttgctt ttacaaagag
     5161 agttatgggt caagatgtga ggatggagaa agttgtttca gaaatagaat cagggttttt
     5221 gttagccaac ccttttaaga tcacatgtga gccaattacg actactttga aacgaaaaca
     5281 agaggcagtt tcagcaacca tcattcaacg tgcttataaa aattaccgct tgaggcgaaa
     5341 tgacaaaaat acatcagata ttcatatgat agatggtgac agagatgttc atgctactaa
     5401 agaaggtgcc tattttgaca aagctaagga aaagtcacct attcaaagcc agatctaata
     5461 ccacttacca cctcttttca tatttcttca catatctgaa aaatgttgaa agcctaagcc
     5521 aggaataaaa gaaaagtaga gataataatc agttctttac aaccgatggt aattaagctt
     5581 gtattcacaa gacttcatgc caaattcact ttttagcatt atatctaaca aatcaagaga
     5641 atccttaata ttgctgcagt gagtttaaag tgggttaaag tggccatttg acaatctcat
     5701 atttgttttc tctacatggc ttatatgatg tgtgccttct agggaatgaa gggaagtggt
     5761 gatagagatc agcagcagca ggggctttct tttatatttt atgtataatt taatgggctt
     5821 taagtcacca ctattaagac ttacaaataa gcaaatactt tcctgatgtg ggatggtgaa
     5881 atgctaatgg ccattaaatc ataaacttgc ctagacaaaa gccaattgga agaagggaga
     5941 gagcagttct ttagaaagtg cctttgagat caacctcaga gattcttggg ctgattaaaa
     6001 ctgcatttga aaaagattgg ttgaagctct gtgtttattt ttgtatgttc ttgttttcat
     6061 ttggaactgg gaatgaatag gatttcattg tgctcaagct cctggtttct catctctgga
     6121 tagtttcacc taagctctgg ctcttaagca ggacagattc gtaaaacaag aagcataaag
     6181 gagaggtata gccttttttt tttttttttt tttcattttc ttctcattta cacctatttt
     6241 tttaaaaagt atacatttac taaaatgatg taataaataa catgttaata gactcaagct
     6301 ttaccttatg aaattgatgt atttttacca gttatttcta atgtaacatt gaatatataa
     6361 gatctgacaa atgtatgttt aaacatgaat tagaagagtt gagaactacc attatgtata
     6421 gggattctca tagtgtcttg gcccttaatt ggaaagttgt ggcaacttta aagtactttt
     6481 tactgtatgt tataattctt tataacttag agagagacaa tggtcactca aactatgaga
     6541 actatgaatt aggagataaa agtttaaatt tgttgttgtt ttataacagt atgtacaagt
     6601 tagttttccc ttatatattt acgttttcaa gttttttaat ctcatcatat acatccatac
     6661 tctataaaat gttttatatt caaagaactg taaaatccta aacattagtt ttcactattg
     6721 aaattgtttt ttaaagatag gcataaatag ttgtccttag acttattcat acaaatatag
     6781 tcatttactt ctatgtagtt tgagattctg agagttattc caactttatg aagattgatt
     6841 tcaatgtgcc tgctaagtcc taaaagattc agaaagaaaa tttatatatt attgatttaa
     6901 atatcatcct ttaaatatgt tgtataacat tcaatatagt ttatgtatca gtgattgtat
     6961 tttattctga atgcatgatc tcaagcctta actactataa tctttttctg cccctcagaa
     7021 attgaataac ctaaccaaga tgcctttagg ggatgcccta agtaaatgta atttcagatt
     7081 tcagggtttt ttttttttcc tctctaagtg ttccttccct ttcttctcct gctctccatc
     7141 atgttatgga gaccagtgag gaaccagtgt taacttggtg acaatgtgac agctggtgct
     7201 ttatctaagc tccgttttct atttcttggg aatgctttat tgtggaaact gcttcagata
     7261 cttaaattga atcataactt gcttctgtaa attgcgtaaa gacaacaaac tgattttagt
     7321 ttgaaaagtt tatcttttac ttgtaaacct tgtttgccag ttaccttccg aaagctgtgt
     7381 aaagagttat ttttaacaaa gtcttaacaa tatatgttac tttttagata ctatagaaaa
     7441 taataaatat aacctgtaaa ccaca
//
