LOCUS       NM_000335               8516 bp    mRNA    linear   PRI 12-FEB-2025
DEFINITION  Homo sapiens sodium voltage-gated channel alpha subunit 5 (SCN5A),
            transcript variant 2, mRNA.
ACCESSION   NM_000335 XM_001131636
VERSION     NM_000335.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8516)
  AUTHORS   Hu,Y., Zhang,C., Wang,S., Xiong,H., Xie,W., Zeng,Z., Cai,H.,
            Wang,Q.K. and Lu,Z.
  TITLE     14-3-3epsilon/YWHAE regulates the transcriptional expression of
            cardiac sodium channel NaV1.5
  JOURNAL   Heart Rhythm 21 (11), 2320-2329 (2024)
   PUBMED   38750908
  REMARK    GeneRIF: 14-3-3epsilon/YWHAE regulates the transcriptional
            expression of cardiac sodium channel NaV1.5.
REFERENCE   2  (bases 1 to 8516)
  AUTHORS   Cadena-Ullauri,S., Guevara-Ramirez,P., Ruiz-Pozo,V.A.,
            Tamayo-Trujillo,R., Paz-Cruz,E., Simancas-Racines,D.,
            Ibarra-Castillo,R., Laso-Bayas,J.L. and Zambrano,A.K.
  TITLE     Genomic analysis of an Ecuadorian individual carrying an SCN5A rare
            variant
  JOURNAL   BMC Cardiovasc Disord 24 (1), 388 (2024)
   PUBMED   39068398
  REMARK    GeneRIF: Genomic analysis of an Ecuadorian individual carrying an
            SCN5A rare variant.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 8516)
  AUTHORS   Tariq,U., Sarkar,S., Malladi,N., Kumar,R., Bugga,P., Chakraborty,P.
            and Banerjee,S.K.
  TITLE     Knockdown of SCN5A alters metabolic-associated genes and aggravates
            hypertrophy in the cardiomyoblast
  JOURNAL   Mol Biol Rep 51 (1), 661 (2024)
   PUBMED   38758505
  REMARK    GeneRIF: Knockdown of SCN5A alters metabolic-associated genes and
            aggravates hypertrophy in the cardiomyoblast.
            Erratum:[Mol Biol Rep. 2024 Jul 26;51(1):852. doi:
            10.1007/s11033-024-09742-9.. PMID: 39060789]
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 8516)
  AUTHORS   Wright,K.M., Nathan,S., Jiang,H., Xia,W., Kim,H., Chakouri,N.,
            Nwafor,J.N., Fossier,L., Srinivasan,L., Chen,Z., Boronina,T.,
            Post,J., Paul,S., Cole,R.N., Ben-Johny,M., Cole,P.A. and
            Gabelli,S.B.
  TITLE     NEDD4L intramolecular interactions regulate its auto and substrate
            NaV1.5 ubiquitination
  JOURNAL   J Biol Chem 300 (3), 105715 (2024)
   PUBMED   38309503
  REMARK    GeneRIF: NEDD4L intramolecular interactions regulate its auto and
            substrate NaV1.5 ubiquitination.
REFERENCE   5  (bases 1 to 8516)
  AUTHORS   George,A.L. Jr., Varkony,T.A., Drabkin,H.A., Han,J., Knops,J.F.,
            Finley,W.H., Brown,G.B., Ward,D.C. and Haas,M.
  TITLE     Assignment of the human heart tetrodotoxin-resistant voltage-gated
            Na+ channel alpha-subunit gene (SCN5A) to band 3p21
  JOURNAL   Cytogenet Cell Genet 68 (1-2), 67-70 (1995)
   PUBMED   7956363
REFERENCE   6  (bases 1 to 8516)
  AUTHORS   Jiang,C., Atkinson,D., Towbin,J.A., Splawski,I., Lehmann,M.H.,
            Li,H., Timothy,K., Taggart,R.T., Schwartz,P.J., Vincent,G.M. et al.
  TITLE     Two long QT syndrome loci map to chromosomes 3 and 7 with evidence
            for further heterogeneity
  JOURNAL   Nat Genet 8 (2), 141-147 (1994)
   PUBMED   7842012
REFERENCE   7  (bases 1 to 8516)
  AUTHORS   Brugada,R., Campuzano,O., Sarquella-Brugada,G., Brugada,P.,
            Brugada,J. and Hong,K.
  TITLE     Brugada Syndrome
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301690
REFERENCE   8  (bases 1 to 8516)
  AUTHORS   Hershberger,R.E. and Jordan,E.
  TITLE     Dilated Cardiomyopathy Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301486
REFERENCE   9  (bases 1 to 8516)
  AUTHORS   Groffen,A.J., Bikker,H. and Christiaans,I.
  TITLE     Long QT Syndrome Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301308
REFERENCE   10 (bases 1 to 8516)
  AUTHORS   Gellens,M.E., George,A.L. Jr., Chen,L.Q., Chahine,M., Horn,R.,
            Barchi,R.L. and Kallen,R.G.
  TITLE     Primary structure and functional expression of the human cardiac
            tetrodotoxin-insensitive voltage-dependent sodium channel
  JOURNAL   Proc Natl Acad Sci U S A 89 (2), 554-558 (1992)
   PUBMED   1309946
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AP006241.1, BU845010.1,
            AF482988.1, AY148488.1, BC051374.1 and AB158469.2.
            
            On Oct 23, 2019 this sequence version replaced NM_000335.4.
            
            Summary: The protein encoded by this gene is an integral membrane
            protein and tetrodotoxin-resistant voltage-gated sodium channel
            subunit. This protein is found primarily in cardiac muscle and is
            responsible for the initial upstroke of the action potential in an
            electrocardiogram. Defects in this gene have been associated with
            long QT syndrome type 3 (LQT3), atrial fibrillation,
            cardiomyopathy, and Brugada syndrome 1, all autosomal dominant
            cardiac diseases. Alternative splicing results in several
            transcript variants encoding different isoforms. [provided by
            RefSeq, May 2022].
            
            Transcript Variant: This variant (2) uses a different acceptor
            splice site at one of the coding exons, 3 nt downstream of that
            used by transcript variant 1. This results in an isoform (b)
            shorter by just a single aa, compared to isoform a.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            CDS exon combination :: AY148488.1, AF482988.1 [ECO:0000331]
            RNAseq introns       :: mixed sample support SAMEA1968189,
                                    SAMEA1968540 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000423572.7/ ENSP00000398266.2
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-15                AP006241.1         157853-157867       c
            16-200              BU845010.1         1-185
            201-209             AF482988.1         1-9
            210-230             AY148488.1         1-21
            231-295             BC051374.1         217-281
            296-780             AB158469.2         129-613
            781-4096            AY148488.1         572-3887
            4097-4641           AB158469.2         3933-4477
            4642-6331           AY148488.1         4433-6122
            6332-8516           AP006241.1         56242-58426         c
FEATURES             Location/Qualifiers
     source          1..8516
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p22.2"
     gene            1..8516
                     /gene="SCN5A"
                     /gene_synonym="CDCD2; CMD1E; CMPD2; HB1; HB2; HBBD; HH1;
                     ICCD; IVF; LQT3; Nav1.5; PFHB1; SSS1; VF1"
                     /note="sodium voltage-gated channel alpha subunit 5"
                     /db_xref="GeneID:6331"
                     /db_xref="HGNC:HGNC:10593"
                     /db_xref="MIM:600163"
     exon            1..157
                     /gene="SCN5A"
                     /gene_synonym="CDCD2; CMD1E; CMPD2; HB1; HB2; HBBD; HH1;
                     ICCD; IVF; LQT3; Nav1.5; PFHB1; SSS1; VF1"
                     /inference="alignment:Splign:2.1.0"
     exon            158..482
                     /gene="SCN5A"
                     /gene_synonym="CDCD2; CMD1E; CMPD2; HB1; HB2; HBBD; HH1;
                     ICCD; IVF; LQT3; Nav1.5; PFHB1; SSS1; VF1"
                     /inference="alignment:Splign:2.1.0"
     CDS             210..6257
                     /gene="SCN5A"
                     /gene_synonym="CDCD2; CMD1E; CMPD2; HB1; HB2; HBBD; HH1;
                     ICCD; IVF; LQT3; Nav1.5; PFHB1; SSS1; VF1"
                     /note="isoform b is encoded by transcript variant 2;
                     cardiac tetrodotoxin-insensitive voltage-dependent sodium
                     channel alpha subunit; sodium channel protein type 5
                     subunit alpha; voltage-gated sodium channel subunit alpha
                     Nav1.5; sodium channel protein cardiac muscle subunit
                     alpha; sodium channel, voltage-gated, type V, alpha
                     subunit"
                     /codon_start=1
                     /product="sodium channel protein type 5 subunit alpha
                     isoform b"
                     /protein_id="NP_000326.2"
                     /db_xref="CCDS:CCDS46797.1"
                     /db_xref="GeneID:6331"
                     /db_xref="HGNC:HGNC:10593"
                     /db_xref="MIM:600163"
                     /translation="MANFLLPRGTSSFRRFTRESLAAIEKRMAEKQARGSTTLQESRE
                     GLPEEEAPRPQLDLQASKKLPDLYGNPPQELIGEPLEDLDPFYSTQKTFIVLNKGKTI
                     FRFSATNALYVLSPFHPIRRAAVKILVHSLFNMLIMCTILTNCVFMAQHDPPPWTKYV
                     EYTFTAIYTFESLVKILARGFCLHAFTFLRDPWNWLDFSVIIMAYTTEFVDLGNVSAL
                     RTFRVLRALKTISVISGLKTIVGALIQSVKKLADVMVLTVFCLSVFALIGLQLFMGNL
                     RHKCVRNFTALNGTNGSVEADGLVWESLDLYLSDPENYLLKNGTSDVLLCGNSSDAGT
                     CPEGYRCLKAGENPDHGYTSFDSFAWAFLALFRLMTQDCWERLYQQTLRSAGKIYMIF
                     FMLVIFLGSFYLVNLILAVVAMAYEEQNQATIAETEEKEKRFQEAMEMLKKEHEALTI
                     RGVDTVSRSSLEMSPLAPVNSHERRSKRRKRMSSGTEECGEDRLPKSDSEDGPRAMNH
                     LSLTRGLSRTSMKPRSSRGSIFTFRRRDLGSEADFADDENSTAGESESHHTSLLVPWP
                     LRRTSAQGQPSPGTSAPGHALHGKKNSTVDCNGVVSLLGAGDPEATSPGSHLLRPVML
                     EHPPDTTTPSEEPGGPQMLTSQAPCVDGFEEPGARQRALSAVSVLTSALEELEESRHK
                     CPPCWNRLAQRYLIWECCPLWMSIKQGVKLVVMDPFTDLTITMCIVLNTLFMALEHYN
                     MTSEFEEMLQVGNLVFTGIFTAEMTFKIIALDPYYYFQQGWNIFDSIIVILSLMELGL
                     SRMSNLSVLRSFRLLRVFKLAKSWPTLNTLIKIIGNSVGALGNLTLVLAIIVFIFAVV
                     GMQLFGKNYSELRDSDSGLLPRWHMMDFFHAFLIIFRILCGEWIETMWDCMEVSGQSL
                     CLLVFLLVMVIGNLVVLNLFLALLLSSFSADNLTAPDEDREMNNLQLALARIQRGLRF
                     VKRTTWDFCCGLLRQRPQKPAALAAQGQLPSCIATPYSPPPPETEKVPPTRKETRFEE
                     GEQPGQGTPGDPEPVCVPIAVAESDTDDQEEDEENSLGTEEESSKQESQPVSGGPEAP
                     PDSRTWSQVSATASSEAEASASQADWRQQWKAEPQAPGCGETPEDSCSEGSTADMTNT
                     AELLEQIPDLGQDVKDPEDCFTEGCVRRCPCCAVDTTQAPGKVWWRLRKTCYHIVEHS
                     WFETFIIFMILLSSGALAFEDIYLEERKTIKVLLEYADKMFTYVFVLEMLLKWVAYGF
                     KKYFTNAWCWLDFLIVDVSLVSLVANTLGFAEMGPIKSLRTLRALRPLRALSRFEGMR
                     VVVNALVGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFGRCINQTEGDLPLNYTIVN
                     NKSQCESLNLTGELYWTKVKVNFDNVGAGYLALLQVATFKGWMDIMYAAVDSRGYEEQ
                     PQWEYNLYMYIYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKLGGQDIFMTEEQKKYY
                     NAMKKLGSKKPQKPIPRPLNKYQGFIFDIVTKQAFDVTIMFLICLNMVTMMVETDDQS
                     PEKINILAKINLLFVAIFTGECIVKLAALRHYYFTNSWNIFDFVVVILSIVGTVLSDI
                     IQKYFFSPTLFRVIRLARIGRILRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLVMF
                     IYSIFGMANFAYVKWEAGIDDMFNFQTFANSMLCLFQITTSAGWDGLLSPILNTGPPY
                     CDPTLPNSNGSRGDCGSPAVGILFFTTYIIISFLIVVNMYIAIILENFSVATEESTEP
                     LSEDDFDMFYEIWEKFDPEATQFIEYSVLSDFADALSEPLRIAKPNQISLINMDLPMV
                     SGDRIHCMDILFAFTKRVLGESGEMDALKIQMEEKFMAANPSKISYEPITTTLRRKHE
                     EVSAMVIQRAFRRHLLQRSLKHASFLFRQQAGSGLSEEDAPEREGLIAYVMSENFSRP
                     LGPPSSSSISSTSFPPSYDSVTRATSDNLQVRGSDYSHSEDLADFPPSPDRDRESIV"
     exon            483..601
                     /gene="SCN5A"
                     /gene_synonym="CDCD2; CMD1E; CMPD2; HB1; HB2; HBBD; HH1;
                     ICCD; IVF; LQT3; Nav1.5; PFHB1; SSS1; VF1"
                     /inference="alignment:Splign:2.1.0"
     exon            602..691
                     /gene="SCN5A"
                     /gene_synonym="CDCD2; CMD1E; CMPD2; HB1; HB2; HBBD; HH1;
                     ICCD; IVF; LQT3; Nav1.5; PFHB1; SSS1; VF1"
                     /inference="alignment:Splign:2.1.0"
     exon            692..820
                     /gene="SCN5A"
                     /gene_synonym="CDCD2; CMD1E; CMPD2; HB1; HB2; HBBD; HH1;
                     ICCD; IVF; LQT3; Nav1.5; PFHB1; SSS1; VF1"
                     /inference="alignment:Splign:2.1.0"
     exon            821..912
                     /gene="SCN5A"
                     /gene_synonym="CDCD2; CMD1E; CMPD2; HB1; HB2; HBBD; HH1;
                     ICCD; IVF; LQT3; Nav1.5; PFHB1; SSS1; VF1"
                     /inference="alignment:Splign:2.1.0"
     exon            913..1143
                     /gene="SCN5A"
                     /gene_synonym="CDCD2; CMD1E; CMPD2; HB1; HB2; HBBD; HH1;
                     ICCD; IVF; LQT3; Nav1.5; PFHB1; SSS1; VF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1144..1207
                     /gene="SCN5A"
                     /gene_synonym="CDCD2; CMD1E; CMPD2; HB1; HB2; HBBD; HH1;
                     ICCD; IVF; LQT3; Nav1.5; PFHB1; SSS1; VF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1208..1349
                     /gene="SCN5A"
                     /gene_synonym="CDCD2; CMD1E; CMPD2; HB1; HB2; HBBD; HH1;
                     ICCD; IVF; LQT3; Nav1.5; PFHB1; SSS1; VF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1350..1547
                     /gene="SCN5A"
                     /gene_synonym="CDCD2; CMD1E; CMPD2; HB1; HB2; HBBD; HH1;
                     ICCD; IVF; LQT3; Nav1.5; PFHB1; SSS1; VF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1548..1727
                     /gene="SCN5A"
                     /gene_synonym="CDCD2; CMD1E; CMPD2; HB1; HB2; HBBD; HH1;
                     ICCD; IVF; LQT3; Nav1.5; PFHB1; SSS1; VF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1728..2099
                     /gene="SCN5A"
                     /gene_synonym="CDCD2; CMD1E; CMPD2; HB1; HB2; HBBD; HH1;
                     ICCD; IVF; LQT3; Nav1.5; PFHB1; SSS1; VF1"
                     /inference="alignment:Splign:2.1.0"
     exon            2100..2232
                     /gene="SCN5A"
                     /gene_synonym="CDCD2; CMD1E; CMPD2; HB1; HB2; HBBD; HH1;
                     ICCD; IVF; LQT3; Nav1.5; PFHB1; SSS1; VF1"
                     /inference="alignment:Splign:2.1.0"
     exon            2233..2471
                     /gene="SCN5A"
                     /gene_synonym="CDCD2; CMD1E; CMPD2; HB1; HB2; HBBD; HH1;
                     ICCD; IVF; LQT3; Nav1.5; PFHB1; SSS1; VF1"
                     /inference="alignment:Splign:2.1.0"
     exon            2472..2645
                     /gene="SCN5A"
                     /gene_synonym="CDCD2; CMD1E; CMPD2; HB1; HB2; HBBD; HH1;
                     ICCD; IVF; LQT3; Nav1.5; PFHB1; SSS1; VF1"
                     /inference="alignment:Splign:2.1.0"
     exon            2646..2996
                     /gene="SCN5A"
                     /gene_synonym="CDCD2; CMD1E; CMPD2; HB1; HB2; HBBD; HH1;
                     ICCD; IVF; LQT3; Nav1.5; PFHB1; SSS1; VF1"
                     /inference="alignment:Splign:2.1.0"
     exon            2997..3437
                     /gene="SCN5A"
                     /gene_synonym="CDCD2; CMD1E; CMPD2; HB1; HB2; HBBD; HH1;
                     ICCD; IVF; LQT3; Nav1.5; PFHB1; SSS1; VF1"
                     /inference="alignment:Splign:2.1.0"
     exon            3438..3596
                     /gene="SCN5A"
                     /gene_synonym="CDCD2; CMD1E; CMPD2; HB1; HB2; HBBD; HH1;
                     ICCD; IVF; LQT3; Nav1.5; PFHB1; SSS1; VF1"
                     /inference="alignment:Splign:2.1.0"
     exon            3597..3717
                     /gene="SCN5A"
                     /gene_synonym="CDCD2; CMD1E; CMPD2; HB1; HB2; HBBD; HH1;
                     ICCD; IVF; LQT3; Nav1.5; PFHB1; SSS1; VF1"
                     /inference="alignment:Splign:2.1.0"
     exon            3718..3872
                     /gene="SCN5A"
                     /gene_synonym="CDCD2; CMD1E; CMPD2; HB1; HB2; HBBD; HH1;
                     ICCD; IVF; LQT3; Nav1.5; PFHB1; SSS1; VF1"
                     /inference="alignment:Splign:2.1.0"
     exon            3873..4046
                     /gene="SCN5A"
                     /gene_synonym="CDCD2; CMD1E; CMPD2; HB1; HB2; HBBD; HH1;
                     ICCD; IVF; LQT3; Nav1.5; PFHB1; SSS1; VF1"
                     /inference="alignment:Splign:2.1.0"
     exon            4047..4169
                     /gene="SCN5A"
                     /gene_synonym="CDCD2; CMD1E; CMPD2; HB1; HB2; HBBD; HH1;
                     ICCD; IVF; LQT3; Nav1.5; PFHB1; SSS1; VF1"
                     /inference="alignment:Splign:2.1.0"
     exon            4170..4451
                     /gene="SCN5A"
                     /gene_synonym="CDCD2; CMD1E; CMPD2; HB1; HB2; HBBD; HH1;
                     ICCD; IVF; LQT3; Nav1.5; PFHB1; SSS1; VF1"
                     /inference="alignment:Splign:2.1.0"
     exon            4452..4505
                     /gene="SCN5A"
                     /gene_synonym="CDCD2; CMD1E; CMPD2; HB1; HB2; HBBD; HH1;
                     ICCD; IVF; LQT3; Nav1.5; PFHB1; SSS1; VF1"
                     /inference="alignment:Splign:2.1.0"
     exon            4506..4643
                     /gene="SCN5A"
                     /gene_synonym="CDCD2; CMD1E; CMPD2; HB1; HB2; HBBD; HH1;
                     ICCD; IVF; LQT3; Nav1.5; PFHB1; SSS1; VF1"
                     /inference="alignment:Splign:2.1.0"
     exon            4644..4748
                     /gene="SCN5A"
                     /gene_synonym="CDCD2; CMD1E; CMPD2; HB1; HB2; HBBD; HH1;
                     ICCD; IVF; LQT3; Nav1.5; PFHB1; SSS1; VF1"
                     /inference="alignment:Splign:2.1.0"
     exon            4749..5019
                     /gene="SCN5A"
                     /gene_synonym="CDCD2; CMD1E; CMPD2; HB1; HB2; HBBD; HH1;
                     ICCD; IVF; LQT3; Nav1.5; PFHB1; SSS1; VF1"
                     /inference="alignment:Splign:2.1.0"
     exon            5020..8516
                     /gene="SCN5A"
                     /gene_synonym="CDCD2; CMD1E; CMPD2; HB1; HB2; HBBD; HH1;
                     ICCD; IVF; LQT3; Nav1.5; PFHB1; SSS1; VF1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8499..8504
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SCN5A"
                     /gene_synonym="CDCD2; CMD1E; CMPD2; HB1; HB2; HBBD; HH1;
                     ICCD; IVF; LQT3; Nav1.5; PFHB1; SSS1; VF1"
                     /note="hexamer: AATAAA"
     polyA_site      8516
                     /gene="SCN5A"
                     /gene_synonym="CDCD2; CMD1E; CMPD2; HB1; HB2; HBBD; HH1;
                     ICCD; IVF; LQT3; Nav1.5; PFHB1; SSS1; VF1"
                     /note="major polyA site"
ORIGIN      
        1 agagccgcgg agccgagacg gcggcggcgc ccgtaggatg cagggatcgc tcccccgggg
       61 ccgctgagcc tgcgcccagt gccccgagcc ccgcgccgag ccgagtccgc gccaagcagc
      121 agccgcccac cccggggccc ggccggggga ccagcagctt ccccacaggc aacgtgagga
      181 gagcctgtgc ccagaagcag gatgagaaga tggcaaactt cctattacct cggggcacca
      241 gcagcttccg caggttcaca cgggagtccc tggcagccat cgagaagcgc atggcagaga
      301 agcaagcccg cggctcaacc accttgcagg agagccgaga ggggctgccc gaggaggagg
      361 ctccccggcc ccagctggac ctgcaggcct ccaaaaagct gccagatctc tatggcaatc
      421 caccccaaga gctcatcgga gagcccctgg aggacctgga ccccttctat agcacccaaa
      481 agactttcat cgtactgaat aaaggcaaga ccatcttccg gttcagtgcc accaacgcct
      541 tgtatgtcct cagtcccttc caccccatcc ggagagcggc tgtgaagatt ctggttcact
      601 cgctcttcaa catgctcatc atgtgcacca tcctcaccaa ctgcgtgttc atggcccagc
      661 acgaccctcc accctggacc aagtatgtcg agtacacctt caccgccatt tacacctttg
      721 agtctctggt caagattctg gctcgaggct tctgcctgca cgcgttcact ttccttcggg
      781 acccatggaa ctggctggac tttagtgtga ttatcatggc atacacaact gaatttgtgg
      841 acctgggcaa tgtctcagcc ttacgcacct tccgagtcct ccgggccctg aaaactatat
      901 cagtcatttc agggctgaag accatcgtgg gggccctgat ccagtctgtg aagaagctgg
      961 ctgatgtgat ggtcctcaca gtcttctgcc tcagcgtctt tgccctcatc ggcctgcagc
     1021 tcttcatggg caacctaagg cacaagtgcg tgcgcaactt cacagcgctc aacggcacca
     1081 acggctccgt ggaggccgac ggcttggtct gggaatccct ggacctttac ctcagtgatc
     1141 cagaaaatta cctgctcaag aacggcacct ctgatgtgtt actgtgtggg aacagctctg
     1201 acgctgggac atgtccggag ggctaccggt gcctaaaggc aggcgagaac cccgaccacg
     1261 gctacaccag cttcgattcc tttgcctggg cctttcttgc actcttccgc ctgatgacgc
     1321 aggactgctg ggagcgcctc tatcagcaga ccctcaggtc cgcagggaag atctacatga
     1381 tcttcttcat gcttgtcatc ttcctggggt ccttctacct ggtgaacctg atcctggccg
     1441 tggtcgcaat ggcctatgag gagcaaaacc aagccaccat cgctgagacc gaggagaagg
     1501 aaaagcgctt ccaggaggcc atggaaatgc tcaagaaaga acacgaggcc ctcaccatca
     1561 ggggtgtgga taccgtgtcc cgtagctcct tggagatgtc ccctttggcc ccagtaaaca
     1621 gccatgagag aagaagcaag aggagaaaac ggatgtcttc aggaactgag gagtgtgggg
     1681 aggacaggct ccccaagtct gactcagaag atggtcccag agcaatgaat catctcagcc
     1741 tcacccgtgg cctcagcagg acttctatga agccacgttc cagccgcggg agcattttca
     1801 cctttcgcag gcgagacctg ggttctgaag cagattttgc agatgatgaa aacagcacag
     1861 cgggggagag cgagagccac cacacatcac tgctggtgcc ctggcccctg cgccggacca
     1921 gtgcccaggg acagcccagt cccggaacct cggctcctgg ccacgccctc catggcaaaa
     1981 agaacagcac tgtggactgc aatggggtgg tctcattact gggggcaggc gacccagagg
     2041 ccacatcccc aggaagccac ctcctccgcc ctgtgatgct agagcacccg ccagacacga
     2101 ccacgccatc ggaggagcca ggcgggcccc agatgctgac ctcccaggct ccgtgtgtag
     2161 atggcttcga ggagccagga gcacggcagc gggccctcag cgcagtcagc gtcctcacca
     2221 gcgcactgga agagttagag gagtctcgcc acaagtgtcc accatgctgg aaccgtctcg
     2281 cccagcgcta cctgatctgg gagtgctgcc cgctgtggat gtccatcaag cagggagtga
     2341 agttggtggt catggacccg tttactgacc tcaccatcac tatgtgcatc gtactcaaca
     2401 cactcttcat ggcgctggag cactacaaca tgacaagtga attcgaggag atgctgcagg
     2461 tcggaaacct ggtcttcaca gggattttca cagcagagat gaccttcaag atcattgccc
     2521 tcgaccccta ctactacttc caacagggct ggaacatctt cgacagcatc atcgtcatcc
     2581 ttagcctcat ggagctgggc ctgtcccgca tgagcaactt gtcggtgctg cgctccttcc
     2641 gcctgctgcg ggtcttcaag ctggccaaat catggcccac cctgaacaca ctcatcaaga
     2701 tcatcgggaa ctcagtgggg gcactgggga acctgacact ggtgctagcc atcatcgtgt
     2761 tcatctttgc tgtggtgggc atgcagctct ttggcaagaa ctactcggag ctgagggaca
     2821 gcgactcagg cctgctgcct cgctggcaca tgatggactt ctttcatgcc ttcctcatca
     2881 tcttccgcat cctctgtgga gagtggatcg agaccatgtg ggactgcatg gaggtgtcgg
     2941 ggcagtcatt atgcctgctg gtcttcttgc ttgttatggt cattggcaac cttgtggtcc
     3001 tgaatctctt cctggccttg ctgctcagct ccttcagtgc agacaacctc acagcccctg
     3061 atgaggacag agagatgaac aacctccagc tggccctggc ccgcatccag aggggcctgc
     3121 gctttgtcaa gcggaccacc tgggatttct gctgtggtct cctgcggcag cggcctcaga
     3181 agcccgcagc ccttgccgcc cagggccagc tgcccagctg cattgccacc ccctactccc
     3241 cgccaccccc agagacggag aaggtgcctc ccacccgcaa ggaaacacgg tttgaggaag
     3301 gcgagcaacc aggccagggc acccccgggg atccagagcc cgtgtgtgtg cccatcgctg
     3361 tggccgagtc agacacagat gaccaagaag aagatgagga gaacagcctg ggcacggagg
     3421 aggagtccag caagcaggaa tcccagcctg tgtccggtgg cccagaggcc cctccggatt
     3481 ccaggacctg gagccaggtg tcagcgactg cctcctctga ggccgaggcc agtgcatctc
     3541 aggccgactg gcggcagcag tggaaagcgg aaccccaggc cccagggtgc ggtgagaccc
     3601 cagaggacag ttgctccgag ggcagcacag cagacatgac caacaccgct gagctcctgg
     3661 agcagatccc tgacctcggc caggatgtca aggacccaga ggactgcttc actgaaggct
     3721 gtgtccggcg ctgtccctgc tgtgcggtgg acaccacaca ggccccaggg aaggtctggt
     3781 ggcggttgcg caagacctgc taccacatcg tggagcacag ctggttcgag acattcatca
     3841 tcttcatgat cctactcagc agtggagcgc tggccttcga ggacatctac ctagaggagc
     3901 ggaagaccat caaggttctg cttgagtatg ccgacaagat gttcacatat gtcttcgtgc
     3961 tggagatgct gctcaagtgg gtggcctacg gcttcaagaa gtacttcacc aatgcctggt
     4021 gctggctcga cttcctcatc gtagacgtct ctctggtcag cctggtggcc aacaccctgg
     4081 gctttgccga gatgggcccc atcaagtcac tgcggacgct gcgtgcactc cgtcctctga
     4141 gagctctgtc acgatttgag ggcatgaggg tggtggtcaa tgccctggtg ggcgccatcc
     4201 cgtccatcat gaacgtcctc ctcgtctgcc tcatcttctg gctcatcttc agcatcatgg
     4261 gcgtgaacct ctttgcgggg aagtttggga ggtgcatcaa ccagacagag ggagacttgc
     4321 ctttgaacta caccatcgtg aacaacaaga gccagtgtga gtccttgaac ttgaccggag
     4381 aattgtactg gaccaaggtg aaagtcaact ttgacaacgt gggggccggg tacctggccc
     4441 ttctgcaggt ggcaacattt aaaggctgga tggacattat gtatgcagct gtggactcca
     4501 gggggtatga agagcagcct cagtgggaat acaacctcta catgtacatc tattttgtca
     4561 ttttcatcat ctttgggtct ttcttcaccc tgaacctctt tattggtgtc atcattgaca
     4621 acttcaacca acagaagaaa aagttagggg gccaggacat cttcatgaca gaggagcaga
     4681 agaagtacta caatgccatg aagaagctgg gctccaagaa gccccagaag cccatcccac
     4741 ggcccctgaa caagtaccag ggcttcatat tcgacattgt gaccaagcag gcctttgacg
     4801 tcaccatcat gtttctgatc tgcttgaata tggtgaccat gatggtggag acagatgacc
     4861 aaagtcctga gaaaatcaac atcttggcca agatcaacct gctctttgtg gccatcttca
     4921 caggcgagtg tattgtcaag ctggctgccc tgcgccacta ctacttcacc aacagctgga
     4981 atatcttcga cttcgtggtt gtcatcctct ccatcgtggg cactgtgctc tcggacatca
     5041 tccagaagta cttcttctcc ccgacgctct tccgagtcat ccgcctggcc cgaataggcc
     5101 gcatcctcag actgatccga ggggccaagg ggatccgcac gctgctcttt gccctcatga
     5161 tgtccctgcc tgccctcttc aacatcgggc tgctgctctt cctcgtcatg ttcatctact
     5221 ccatctttgg catggccaac ttcgcttatg tcaagtggga ggctggcatc gacgacatgt
     5281 tcaacttcca gaccttcgcc aacagcatgc tgtgcctctt ccagatcacc acgtcggccg
     5341 gctgggatgg cctcctcagc cccatcctca acactgggcc gccctactgc gaccccactc
     5401 tgcccaacag caatggctct cggggggact gcgggagccc agccgtgggc atcctcttct
     5461 tcaccaccta catcatcatc tccttcctca tcgtggtcaa catgtacatt gccatcatcc
     5521 tggagaactt cagcgtggcc acggaggaga gcaccgagcc cctgagtgag gacgacttcg
     5581 atatgttcta tgagatctgg gagaaatttg acccagaggc cactcagttt attgagtatt
     5641 cggtcctgtc tgactttgcc gatgccctgt ctgagccact ccgtatcgcc aagcccaacc
     5701 agataagcct catcaacatg gacctgccca tggtgagtgg ggaccgcatc cattgcatgg
     5761 acattctctt tgccttcacc aaaagggtcc tgggggagtc tggggagatg gacgccctga
     5821 agatccagat ggaggagaag ttcatggcag ccaacccatc caagatctcc tacgagccca
     5881 tcaccaccac actccggcgc aagcacgaag aggtgtcggc catggttatc cagagagcct
     5941 tccgcaggca cctgctgcaa cgctctttga agcatgcctc cttcctcttc cgtcagcagg
     6001 cgggcagcgg cctctccgaa gaggatgccc ctgagcgaga gggcctcatc gcctacgtga
     6061 tgagtgagaa cttctcccga ccccttggcc caccctccag ctcctccatc tcctccactt
     6121 ccttcccacc ctcctatgac agtgtcacta gagccaccag cgataacctc caggtgcggg
     6181 ggtctgacta cagccacagt gaagatctcg ccgacttccc cccttctccg gacagggacc
     6241 gtgagtccat cgtgtgagcc tcggcctggc tggccaggac acactgaaaa gcagcctttt
     6301 tcaccatggc aaacctaaat gcagtcagtc acaaaccagc ctggggcctt cctggctttg
     6361 ggagtaagaa atgggcctca gccccgcgga tcaaccaggc agagttctgt ggcgccgcgt
     6421 ggacagccgg agcagttggc ctgtgcttgg aggcctcaga tagacctgtg acctggtctg
     6481 gtcaggcaat gccctgcggc tctggaaagc aacttcatcc cagctgctga ggcgaaatat
     6541 aaaactgaga ctgtatatgt tgtgaatggg ctttcataaa tttattatat ttgatatttt
     6601 tttacttgag caaagaacta aggatttttc catggacatg ggcagcaatt cacgctgtct
     6661 cttcttaacc ctgaacaaga gtgtctatgg agcagccgga agtctgttct caaagcagaa
     6721 gtggaatcca gtgtggctcc cacaggtctt cactgcccag gggtcgaatg gggtccccct
     6781 cccacttgac ctgagatgct gggagggctg aacccccact cacacaagca cacacacaca
     6841 gtcctcacac acggaggcca gacacaggcc gtgggaccca ggctcccagc ctaagggaga
     6901 caggcctttc cctgccggcc ccccaaggat ggggttcttg tccacggggc tcactctggc
     6961 cccctattgt ctccaaggtc ccattttccc cctgtgtttt cacgcaggtc atattgtcag
     7021 tcctacaaaa ataaaaggct tccagaggag agtggcctgg gtcccagggc tggccctagg
     7081 cactgatagt tgccttttct tcccctcctg taagagtatt aacaaaacca aaggacacaa
     7141 gggtgcaagc cccattcacg gcctggcatg cagcttgtcc ttgctcctgg aacctggcag
     7201 gccctgccca gccagccatc ggaagagagg gctgagccat gggggtttgg ggctaagaag
     7261 ttcaccagcc ctgagccatg gcggcccctc agcctgcctg aagagaggaa actggcgatc
     7321 tcccagggct ctctggacca tacgcggagg agttttctgt gtggtctcca gctcctctcc
     7381 agacacagag acatgggagt ggggagcgga gcttggccct gcgccctgtg cagggaaagg
     7441 gatggtcagg cccagttctc gtgcccttag aggggaatga accatggcac ctttgagaga
     7501 gggggcactg tggtcaggcc cagcctctct ggctcagccc gggatcctga tggcacccac
     7561 acagaggacc tctttggggc aagatccagg tggtcccata ggtcttgtga aaaggctttt
     7621 tcagggaaaa atattttact agtccaatca cccccaggac ctcttcagct gctgacaatc
     7681 ctatttagca tatgcaaatc ttttaacata gagaactgtc accctgaggt aacagggtca
     7741 actggcgaag cctgagcagg caggggcttg gctgccccat tccagctctc ccatggagcc
     7801 cctccaccgg gcgcatgcct cccaggccac ctcagtctca cctgccggct ctgggctggc
     7861 tgctcctaac ctacctcgcc gagctgtcgg agggctggac atttgtggca gtgctgaagg
     7921 gggcattgcc ggcgagtaaa gtattatgtt tcttcttgtc accccagttc ccttggtggc
     7981 aaccccagac ccaacccatg cccctgacag atctagttct cttctcctgt gttccctttg
     8041 agtccagtgt gggacacggt ttaactgtcc cagcgacatt tctccaagtg gaaatcctat
     8101 ttttgtagat ctccatgctt tgctctcaag gcttggagag gtatgtgccc ctcctgggtg
     8161 ctcaccgcct gctacacagg caggaatgcg gttgggaggc aggtcgggct gccagcccag
     8221 ctggccggaa ggagactgtg gtttttgtgt gtgtggacag cccgggagct ttgagacagg
     8281 tgcctggggc tggctgcaga cggtgtggtt gggggtggga ggtgagctag acccaaccct
     8341 tagcttttag cctggctgtc acctttttaa tttccagaac tgcacaatga ccagcaggag
     8401 ggaaggacag acatcaagtg ccagatgttg tctgaactaa tcgagcactt ctcaccaaac
     8461 ttcatgtata aataaaatac atatttttaa aacaaaccaa taaatggctt acatga
//
