LOCUS       NM_016510               2450 bp    mRNA    linear   PRI 01-JUN-2024
DEFINITION  Homo sapiens selenocysteine lyase (SCLY), mRNA.
ACCESSION   NM_016510
VERSION     NM_016510.7
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Fragoza,R., Das,J., Wierbowski,S.D., Liang,J., Tran,T.N., Liang,S.,
            Beltran,J.F., Rivera-Erick,C.A., Ye,K., Wang,T.Y., Yao,L., Mort,M.,
            Stenson,P.D., Cooper,D.N., Wei,X., Keinan,A., Schimenti,J.C.,
            Clark,A.G. and Yu,H.
  TITLE     Extensive disruption of protein interactions by genetic variants
            across the allele frequency spectrum in human populations
  JOURNAL   Nat Commun 10 (1), 4141 (2019)
   PUBMED   31515488
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Chen,S., Fragoza,R., Klei,L., Liu,Y., Wang,J., Roeder,K., Devlin,B.
            and Yu,H.
  TITLE     An interactome perturbation framework prioritizes damaging missense
            mutations for developmental disorders
  JOURNAL   Nat Genet 50 (7), 1032-1040 (2018)
   PUBMED   29892012
REFERENCE   3  (bases 1 to 2450)
  AUTHORS   Collins,R., Johansson,A.L., Karlberg,T., Markova,N., van den
            Berg,S., Olesen,K., Hammarstrom,M., Flores,A., Schuler,H.,
            Schiavone,L.H., Brzezinski,P., Arner,E.S. and Hogbom,M.
  TITLE     Biochemical discrimination between selenium and sulfur 1: a single
            residue provides selenium specificity to human selenocysteine lyase
  JOURNAL   PLoS One 7 (1), e30581 (2012)
   PUBMED   22295093
  REMARK    GeneRIF: the X-ray crystal structure of human selenocysteine lyase
            and the key residue that provides the selenocysteine specificity
REFERENCE   4  (bases 1 to 2450)
  AUTHORS   Johansson,A.L., Collins,R., Arner,E.S., Brzezinski,P. and Hogbom,M.
  TITLE     Biochemical discrimination between selenium and sulfur 2:
            mechanistic investigation of the selenium specificity of human
            selenocysteine lyase
  JOURNAL   PLoS One 7 (1), e30528 (2012)
   PUBMED   22291978
  REMARK    GeneRIF: A reaction mechanism whereby the Selenocysteine over
            Cysteine specificity is achieved using a combination of chemical
            and physico-mechanical control mechanisms.
REFERENCE   5  (bases 1 to 2450)
  AUTHORS   Yu,H., Tardivo,L., Tam,S., Weiner,E., Gebreab,F., Fan,C.,
            Svrzikapa,N., Hirozane-Kishikawa,T., Rietman,E., Yang,X.,
            Sahalie,J., Salehi-Ashtiani,K., Hao,T., Cusick,M.E., Hill,D.E.,
            Roth,F.P., Braun,P. and Vidal,M.
  TITLE     Next-generation sequencing to generate interactome datasets
  JOURNAL   Nat Methods 8 (6), 478-480 (2011)
   PUBMED   21516116
REFERENCE   6  (bases 1 to 2450)
  AUTHORS   Lee,O.H., Kim,H., He,Q., Baek,H.J., Yang,D., Chen,L.Y., Liang,J.,
            Chae,H.K., Safari,A., Liu,D. and Songyang,Z.
  TITLE     Genome-wide YFP fluorescence complementation screen identifies new
            regulators for telomere signaling in human cells
  JOURNAL   Mol Cell Proteomics 10 (2), M110.001628 (2011)
   PUBMED   21044950
REFERENCE   7  (bases 1 to 2450)
  AUTHORS   Yepes,J.O., Luz Gunturiz,M., Henao,L.F., Navas,M.C., Balcazar,N.
            and Gomez,L.A.
  TITLE     [Differential display of messenger RNA and identification of
            selenocysteine lyase gene in hepatocellular carcinoma cells
            transiently expressing hepatitis C virus core protein]
  JOURNAL   Biomedica 26 (2), 194-205 (2006)
   PUBMED   16925092
  REMARK    GeneRIF: Results confirm that HCV core protein is associated with
            specific changes in mRNA expression, including the gene for
            selenocysteine lyase, which may be involved in the pathophysiology
            of hepatocellular carcinoma.
REFERENCE   8  (bases 1 to 2450)
  AUTHORS   Mihara,H., Kurihara,T., Watanabe,T., Yoshimura,T. and Esaki,N.
  TITLE     cDNA cloning, purification, and characterization of mouse liver
            selenocysteine lyase. Candidate for selenium delivery protein in
            selenoprotein synthesis
  JOURNAL   J Biol Chem 275 (9), 6195-6200 (2000)
   PUBMED   10692412
  REMARK    GeneRIF: PMID:10692412 concerns mostly mouse selenocysteine lyase,
            however, refers to the sequence of the N-terminal region of
            putative human protein.
REFERENCE   9  (bases 1 to 2450)
  AUTHORS   Daher,R. and Van Lente,F.
  TITLE     Characterization of selenocysteine lyase in human tissues and its
            relationship to tissue selenium concentrations
  JOURNAL   J Trace Elem Electrolytes Health Dis 6 (3), 189-194 (1992)
   PUBMED   1483038
REFERENCE   10 (bases 1 to 2450)
  AUTHORS   Esaki,N., Nakamura,T., Tanaka,H. and Soda,K.
  TITLE     Selenocysteine lyase, a novel enzyme that specifically acts on
            selenocysteine. Mammalian distribution and purification and
            properties of pig liver enzyme
  JOURNAL   J Biol Chem 257 (8), 4386-4391 (1982)
   PUBMED   6461656
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AB209458.2, BC007891.2, AK293283.1, AC016757.10 and BC018966.2.
            
            On May 17, 2019 this sequence version replaced NM_016510.6.
            
            Summary: Selenocysteine lyase (SCLY; EC 4.4.1.16) catalyzes the
            pyridoxal 5-prime phosphate-dependent conversion of
            L-selenocysteine to L-alanine and elemental selenium (Mihara et
            al., 2000 [PubMed 10692412]).[supplied by OMIM, Mar 2008].
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.1050589.1,
                                           SRR1163655.520968.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: lack of evidence for use of
                                                upstream AUG
            MANE Ensembl match               :: ENST00000254663.12/
                                                ENSP00000254663.7
            RefSeq Select criteria           :: based on single protein-coding
                                                transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-38                AB209458.2         79-116
            39-576              BC007891.2         1-538
            577-1525            AK293283.1         450-1398
            1526-2421           AC016757.10        12708-13603
            2422-2450           BC018966.2         1873-1901
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q37.3"
     gene            1..2450
                     /gene="SCLY"
                     /gene_synonym="hSCL; SCL"
                     /note="selenocysteine lyase"
                     /db_xref="GeneID:51540"
                     /db_xref="HGNC:HGNC:18161"
                     /db_xref="MIM:611056"
     exon            1..143
                     /gene="SCLY"
                     /gene_synonym="hSCL; SCL"
                     /inference="alignment:Splign:2.1.0"
     CDS             55..1392
                     /gene="SCLY"
                     /gene_synonym="hSCL; SCL"
                     /EC_number="4.4.1.16"
                     /codon_start=1
                     /product="selenocysteine lyase"
                     /protein_id="NP_057594.5"
                     /db_xref="CCDS:CCDS2524.3"
                     /db_xref="GeneID:51540"
                     /db_xref="HGNC:HGNC:18161"
                     /db_xref="MIM:611056"
                     /translation="MEAAVAPGRDAPAPAASQPSGCGKHNSPERKVYMDYNATTPLEP
                     EVIQAMTKAMWEAWGNPSSPYSAGRKAKDIINAARESLAKMIGGKPQDIIFTSGGTES
                     NNLVIHSVVKHFHANQTSKGHTGGHHSPVKGAKPHFITSSVEHDSIRLPLEHLVEEQV
                     AAVTFVPVSKVSGQAEVDDILAAVRPTTRLVTIMLANNETGIVMPVPEISQRIKALNQ
                     ERVAAGLPPILVHTDAAQALGKQRVDVEDLGVDFLTIVGHKFYGPRIGALYIRGLGEF
                     TPLYPMLFGGGQERNFRPGTENTPMIAGLGKAAELVTQNCEAYEAHMRDVRDYLEERL
                     EAEFGQKRIHLNSQFPGTQRLPNTCNFSIRGPRLQGHVVLAQCRVLMASVGAACHSDH
                     GDQPSPVLLSYGVPFDVARNALRLSVGRSTTRAEVDLVVQDLKQAVAQLEDQA"
     exon            144..256
                     /gene="SCLY"
                     /gene_synonym="hSCL; SCL"
                     /inference="alignment:Splign:2.1.0"
     exon            257..357
                     /gene="SCLY"
                     /gene_synonym="hSCL; SCL"
                     /inference="alignment:Splign:2.1.0"
     exon            358..538
                     /gene="SCLY"
                     /gene_synonym="hSCL; SCL"
                     /inference="alignment:Splign:2.1.0"
     exon            539..666
                     /gene="SCLY"
                     /gene_synonym="hSCL; SCL"
                     /inference="alignment:Splign:2.1.0"
     exon            667..831
                     /gene="SCLY"
                     /gene_synonym="hSCL; SCL"
                     /inference="alignment:Splign:2.1.0"
     exon            832..938
                     /gene="SCLY"
                     /gene_synonym="hSCL; SCL"
                     /inference="alignment:Splign:2.1.0"
     exon            939..975
                     /gene="SCLY"
                     /gene_synonym="hSCL; SCL"
                     /inference="alignment:Splign:2.1.0"
     exon            976..1059
                     /gene="SCLY"
                     /gene_synonym="hSCL; SCL"
                     /inference="alignment:Splign:2.1.0"
     exon            1060..1162
                     /gene="SCLY"
                     /gene_synonym="hSCL; SCL"
                     /inference="alignment:Splign:2.1.0"
     exon            1163..1238
                     /gene="SCLY"
                     /gene_synonym="hSCL; SCL"
                     /inference="alignment:Splign:2.1.0"
     exon            1239..2450
                     /gene="SCLY"
                     /gene_synonym="hSCL; SCL"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2429..2434
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SCLY"
                     /gene_synonym="hSCL; SCL"
                     /note="hexamer: AATAAA"
     polyA_site      2450
                     /gene="SCLY"
                     /gene_synonym="hSCL; SCL"
                     /note="major polyA site"
ORIGIN      
        1 gcccgtagcg ctccgcggga aggaggctgg atgcccggca gcagtggggc ggggatggag
       61 gcggccgtgg cgccggggag ggatgcgccg gcacccgcgg cgagtcagcc cagcggctgc
      121 gggaaacaca actcgccgga gaggaaagtt tatatggact ataatgcaac gactcccctg
      181 gagccagaag ttatccaggc catgaccaag gccatgtggg aagcctgggg aaatcccagc
      241 agcccgtatt cagcaggaag aaaggccaag gatattataa atgcagctcg ggaaagcctc
      301 gcgaagatga taggggggaa acctcaagat ataatcttca cttccggggg cactgagtca
      361 aataatttag taatccattc tgtggtgaaa catttccacg caaaccagac ctcaaaggga
      421 cacacaggtg ggcaccacag cccagtgaag ggggccaagc cccatttcat tacttcctcg
      481 gtggaacacg actccatccg gctgcccctg gagcacctgg tggaagaaca agtggcagcg
      541 gtcacctttg tcccggtgtc caaggtgagc gggcaggcag aggtggacga catcctcgcg
      601 gcagtccgcc cgaccacacg cctcgtgacc atcatgctgg ccaacaatga gactggcatt
      661 gtcatgcctg tccctgaaat cagtcagcgc attaaagccc tgaaccagga acgggtggca
      721 gctgggctac ctcccatcct cgtgcacacg gatgctgcac aggccttggg gaagcagcgc
      781 gtggatgtgg aggacctggg cgtggacttc cttacaatcg tggggcacaa gttttatggt
      841 cccaggattg gcgcacttta tatacgagga cttggtgaat ttacccctct ctaccctatg
      901 ctatttggag gtggacaaga acggaatttc aggccaggga cagagaacac cccaatgatt
      961 gctggccttg ggaaggccgc ggagctggtg acccagaact gcgaggctta tgaggcccac
     1021 atgagggacg tccgcgacta cctggaagag aggctggaag ctgaattcgg tcagaagaga
     1081 atccatctga atagccagtt tccaggcacc cagcggcttc ccaatacctg taacttttcc
     1141 atccggggac cccggcttca aggccacgtg gtgcttgcgc agtgccgagt gctgatggcc
     1201 agtgtggggg ccgcgtgcca ctcggaccac ggggaccagc cgtccccagt gctgctgagc
     1261 tacggtgtcc ccttcgacgt ggccaggaac gcgctccggc tcagcgtggg ccgcagcacc
     1321 accagggccg aggtggacct cgtcgtgcag gacctgaagc aggccgtggc gcagctggag
     1381 gaccaggcct agcactgggg ccgccttccc caccccgctt ctgggaagcc cgtggcaggg
     1441 cacagggttg tccctccagt tccctcctga gggctgtgcc aggatgactg tctcatgccc
     1501 cctctgcatt ttgtcctgga gtgccagcga gtgtgcaccc ccagtttcct tccctggacc
     1561 cctgcagagc tcacagggcc caggacacca acgccgcata ggactgccca catgggaccg
     1621 cccacatagg accgcccaca taggaccgcc cacatgggac cgcccacatg ggaccgccca
     1681 catgggaccg cccacataga accgtcctcc agtggtgaag cggaaacact tagctttatc
     1741 caccctcccc actgggaact gggcacgcct gttgtgagtg ccctttcctg gaaggtgttt
     1801 ttatctggaa gatagaatcc aagtatttat aactcattgt cagccaaatg ctatcatgaa
     1861 cgtaggaaac ttgatttttt tgttttgatc atggcctcta catgcacctt tccagagtgg
     1921 tctcttctca ggcccttttt agtccctttc caaagctgcc cccaccaccc tgctcctctg
     1981 gcctcagtgc acagtggccc ccagcctcgg ccaggcctgc tctgctcagc gcccaccgcc
     2041 caccacccct cctgcttccc cgagcctgac cctgttccgc ccactggcaa tcagggctgc
     2101 gcacttccct gtccacggtc cccaggcctt cctgtcttgt cccttttgat cattattaac
     2161 tcagggtttc agctccaacc tcgctgagtt ggtgcagctc caggtcattc ctggggtggg
     2221 aatcggatca tccctgactc agcttttacc ttaattttat ttgcagagga ttcttttctc
     2281 aaaatgctct ggcatttgga cacacatcac atgtcgatat ttgcatagga gtcattttca
     2341 gtggaataac atttttaatg tgtggtttta cggttcaagg aactacttga tgattttgag
     2401 gaaacacttg ccagaaacta aattaacgaa taaaagattt cagtgcccga
//
