LOCUS       NM_012235               4254 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens SREBF chaperone (SCAP), transcript variant 1, mRNA.
ACCESSION   NM_012235
VERSION     NM_012235.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4254)
  AUTHORS   Liu,M.H., Lin,X.L. and Xiao,L.L.
  TITLE     SARS-CoV-2 nucleocapsid protein promotes TMAO-induced NLRP3
            inflammasome activation by SCAP-SREBP signaling pathway
  JOURNAL   Tissue Cell 86, 102276 (2024)
   PUBMED   37979395
  REMARK    GeneRIF: SARS-CoV-2 nucleocapsid protein promotes TMAO-induced
            NLRP3 inflammasome activation by SCAP-SREBP signaling pathway.
REFERENCE   2  (bases 1 to 4254)
  AUTHORS   Chandrasekaran,P. and Weiskirchen,R.
  TITLE     The Role of SCAP/SREBP as Central Regulators of Lipid Metabolism in
            Hepatic Steatosis
  JOURNAL   Int J Mol Sci 25 (2), 1109 (2024)
   PUBMED   38256181
  REMARK    GeneRIF: The Role of SCAP/SREBP as Central Regulators of Lipid
            Metabolism in Hepatic Steatosis.
            Review article
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4254)
  AUTHORS   Makokha,G.N., Chayama,K., Hayes,C.N., Abe-Chayama,H., Abuduwaili,M.
            and Hijikata,M.
  TITLE     Deficiency of SCAP inhibits HBV pathogenesis via activation of the
            interferon signaling pathway
  JOURNAL   Virology 585, 248-258 (2023)
   PUBMED   37437369
  REMARK    GeneRIF: Deficiency of SCAP inhibits HBV pathogenesis via
            activation of the interferon signaling pathway.
REFERENCE   4  (bases 1 to 4254)
  AUTHORS   Liu,F., Ma,M., Gao,A., Ma,F., Ma,G., Liu,P., Jia,C., Wang,Y.,
            Donahue,K., Zhang,S., Ong,I.M., Keles,S., Li,L. and Xu,W.
  TITLE     PKM2-TMEM33 axis regulates lipid homeostasis in cancer cells by
            controlling SCAP stability
  JOURNAL   EMBO J 40 (22), e108065 (2021)
   PUBMED   34487377
  REMARK    GeneRIF: PKM2-TMEM33 axis regulates lipid homeostasis in cancer
            cells by controlling SCAP stability.
REFERENCE   5  (bases 1 to 4254)
  AUTHORS   Yan,R., Cao,P., Song,W., Li,Y., Wang,T., Qian,H., Yan,C. and Yan,N.
  TITLE     Structural basis for sterol sensing by Scap and Insig
  JOURNAL   Cell Rep 35 (13), 109299 (2021)
   PUBMED   34192549
  REMARK    GeneRIF: Structural basis for sterol sensing by Scap and Insig.
REFERENCE   6  (bases 1 to 4254)
  AUTHORS   Iwaki,K., Nakajima,T., Ota,N. and Emi,M.
  TITLE     A common Ile796Val polymorphism of the human SREBP
            cleavage-activating protein (SCAP) gene
  JOURNAL   J Hum Genet 44 (6), 421-422 (1999)
   PUBMED   10570919
REFERENCE   7  (bases 1 to 4254)
  AUTHORS   Nakajima,T., Hamakubo,T., Kodama,T., Inazawa,J. and Emi,M.
  TITLE     Genomic structure and chromosomal mapping of the human sterol
            regulatory element binding protein (SREBP) cleavage-activating
            protein (SCAP) gene
  JOURNAL   J Hum Genet 44 (6), 402-407 (1999)
   PUBMED   10570913
REFERENCE   8  (bases 1 to 4254)
  AUTHORS   Nohturfft,A., Brown,M.S. and Goldstein,J.L.
  TITLE     Topology of SREBP cleavage-activating protein, a polytopic membrane
            protein with a sterol-sensing domain
  JOURNAL   J Biol Chem 273 (27), 17243-17250 (1998)
   PUBMED   9642295
REFERENCE   9  (bases 1 to 4254)
  AUTHORS   Sakai,J., Nohturfft,A., Cheng,D., Ho,Y.K., Brown,M.S. and
            Goldstein,J.L.
  TITLE     Identification of complexes between the COOH-terminal domains of
            sterol regulatory element-binding proteins (SREBPs) and SREBP
            cleavage-activating protein
  JOURNAL   J Biol Chem 272 (32), 20213-20221 (1997)
   PUBMED   9242699
REFERENCE   10 (bases 1 to 4254)
  AUTHORS   Hua,X., Nohturfft,A., Goldstein,J.L. and Brown,M.S.
  TITLE     Sterol resistance in CHO cells traced to point mutation in SREBP
            cleavage-activating protein
  JOURNAL   Cell 87 (3), 415-426 (1996)
   PUBMED   8898195
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from D83782.2, BG179218.1 and
            AC099778.2.
            
            On Nov 23, 2018 this sequence version replaced NM_012235.3.
            
            Summary: This gene encodes a protein with a sterol sensing domain
            (SSD) and seven WD domains. In the presence of cholesterol, this
            protein binds to sterol regulatory element binding proteins
            (SREBPs) and mediates their transport from the ER to the Golgi. The
            SREBPs are then proteolytically cleaved and regulate sterol
            biosynthesis. Alternative splicing results in multiple transcript
            variants. [provided by RefSeq, Feb 2016].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: D83782.2 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000265565.10/ ENSP00000265565.5
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-4222              D83782.2           5-4226
            4223-4250           BG179218.1         486-513
            4251-4254           AC099778.2         58198-58201         c
FEATURES             Location/Qualifiers
     source          1..4254
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p21.31"
     gene            1..4254
                     /gene="SCAP"
                     /note="SREBF chaperone"
                     /db_xref="GeneID:22937"
                     /db_xref="HGNC:HGNC:30634"
                     /db_xref="MIM:601510"
     exon            1..143
                     /gene="SCAP"
                     /inference="alignment:Splign:2.1.0"
     exon            144..363
                     /gene="SCAP"
                     /inference="alignment:Splign:2.1.0"
     CDS             242..4081
                     /gene="SCAP"
                     /note="isoform 1 is encoded by transcript variant 1; SREBP
                     cleavage-activating protein; sterol regulatory
                     element-binding protein cleavage-activating protein"
                     /codon_start=1
                     /product="sterol regulatory element-binding protein
                     cleavage-activating protein isoform 1"
                     /protein_id="NP_036367.2"
                     /db_xref="CCDS:CCDS2755.2"
                     /db_xref="GeneID:22937"
                     /db_xref="HGNC:HGNC:30634"
                     /db_xref="MIM:601510"
                     /translation="MTLTERLREKISRAFYNHGLLCASYPIPIILFTGFCILACCYPL
                     LKLPLPGTGPVEFTTPVKDYSPPPVDSDRKQGEPTEQPEWYVGAPVAYVQQIFVKSSV
                     FPWHKNLLAVDVFRSPLSRAFQLVEEIRNHVLRDSSGIRSLEELCLQVTDLLPGLRKL
                     RNLLPEHGCLLLSPGNFWQNDWERFHADPDIIGTIHQHEPKTLQTSATLKDLLFGVPG
                     KYSGVSLYTRKRMVSYTITLVFQHYHAKFLGSLRARLMLLHPSPNCSLRAESLVHVHF
                     KEEIGVAELIPLVTTYIILFAYIYFSTRKIDMVKSKWGLALAAVVTVLSSLLMSVGLC
                     TLFGLTPTLNGGEIFPYLVVVIGLENVLVLTKSVVSTPVDLEVKLRIAQGLSSESWSI
                     MKNMATELGIILIGYFTLVPAIQEFCLFAVVGLVSDFFLQMLFFTTVLSIDIRRMELA
                     DLNKRLPPEACLPSAKPVGQPTRYERQLAVRPSTPHTITLQPSSFRNLRLPKRLRVVY
                     FLARTRLAQRLIMAGTVVWIGILVYTDPAGLRNYLAAQVTEQSPLGEGALAPMPVPSG
                     MLPPSHPDPAFSIFPPDAPKLPENQTSPGESPERGGPAEVVHDSPVPEVTWGPEDEEL
                     WRKLSFRHWPTLFSYYNITLAKRYISLLPVIPVTLRLNPREALEGRHPQDGRSAWPPP
                     GPIPAGHWEAGPKGPGGVQAHGDVTLYKVAALGLATGIVLVLLLLCLYRVLCPRNYGQ
                     LGGGPGRRRRGELPCDDYGYAPPETEIVPLVLRGHLMDIECLASDGMLLVSCCLAGHV
                     CVWDAQTGDCLTRIPRPGRQRRDSGVGSGLEAQESWERLSDGGKAGPEEPGDSPPLRH
                     RPRGPPPPSLFGDQPDLTCLIDTNFSAQPRSSQPTQPEPRHRAVCGRSRDSPGYDFSC
                     LVQRVYQEEGLAAVCTPALRPPSPGPVLSQAPEDEGGSPEKGSPSLAWAPSAEGSIWS
                     LELQGNLIVVGRSSGRLEVWDAIEGVLCCSSEEVSSGITALVFLDKRIVAARLNGSLD
                     FFSLETHTALSPLQFRGTPGRGSSPASPVYSSSDTVACHLTHTVPCAHQKPITALKAA
                     AGRLVTGSQDHTLRVFRLEDSCCLFTLQGHSGAITTVYIDQTMVLASGGQDGAICLWD
                     VLTGSRVSHVFAHRGDVTSLTCTTSCVISSGLDDLISIWDRSTGIKFYSIQQDLGCGA
                     SLGVISDNLLVTGGQGCVSFWDLNYGDLLQTVYLGKNSEAQPARQILVLDNAAIVCNF
                     GSELSLVYVPSVLEKLD"
     exon            364..493
                     /gene="SCAP"
                     /inference="alignment:Splign:2.1.0"
     exon            494..651
                     /gene="SCAP"
                     /inference="alignment:Splign:2.1.0"
     exon            652..872
                     /gene="SCAP"
                     /inference="alignment:Splign:2.1.0"
     exon            873..978
                     /gene="SCAP"
                     /inference="alignment:Splign:2.1.0"
     exon            979..1151
                     /gene="SCAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1152..1278
                     /gene="SCAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1279..1391
                     /gene="SCAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1392..1486
                     /gene="SCAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1487..1585
                     /gene="SCAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1586..1804
                     /gene="SCAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1805..2181
                     /gene="SCAP"
                     /inference="alignment:Splign:2.1.0"
     exon            2182..2370
                     /gene="SCAP"
                     /inference="alignment:Splign:2.1.0"
     exon            2371..2572
                     /gene="SCAP"
                     /inference="alignment:Splign:2.1.0"
     exon            2573..2688
                     /gene="SCAP"
                     /inference="alignment:Splign:2.1.0"
     exon            2689..3211
                     /gene="SCAP"
                     /inference="alignment:Splign:2.1.0"
     exon            3212..3297
                     /gene="SCAP"
                     /inference="alignment:Splign:2.1.0"
     exon            3298..3380
                     /gene="SCAP"
                     /inference="alignment:Splign:2.1.0"
     exon            3381..3547
                     /gene="SCAP"
                     /inference="alignment:Splign:2.1.0"
     exon            3548..3628
                     /gene="SCAP"
                     /inference="alignment:Splign:2.1.0"
     exon            3629..3835
                     /gene="SCAP"
                     /inference="alignment:Splign:2.1.0"
     exon            3836..4254
                     /gene="SCAP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4202..4207
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SCAP"
                     /note="hexamer: ATTAAA"
     polyA_site      4223
                     /gene="SCAP"
                     /note="major polyA site"
     polyA_site      4254
                     /gene="SCAP"
ORIGIN      
        1 gggcacccgg cggccaggag agagagggag ggcgccacgc accggactgc gggccgagag
       61 cgcgcacgcc gcgctccgcc cctgctgccg cccccgtcgc cgccgccgcc gccgccgcag
      121 cttgggaggt gctgccacca caggtacctg cacatgttgt tctttgtcag tgctgtcaag
      181 tgtgtgccag ggtgatccat ggtcactttc cgggatggca gcaaggtgac ttcggctgag
      241 gatgaccctg actgaaaggc tgcgtgagaa gatatctcgg gccttctaca accatgggct
      301 cctctgtgca tcctatccca tccccatcat cctcttcaca gggttctgca tcttagcctg
      361 ctgctaccca ctgctgaaac tccccttgcc aggaacagga cctgtggaat tcaccacccc
      421 tgtgaaggat tactcgcccc cacctgtgga ctctgaccgc aaacaaggag agcctactga
      481 gcagcctgag tggtatgtgg gtgccccggt ggcttatgtc cagcagatat ttgtgaagtc
      541 ctcagtgttt ccctggcaca agaacctcct ggcagtagat gtatttcgtt cacctttgtc
      601 ccgggcattc caactggtgg aggagatccg gaaccacgtg ctgagagaca gctctgggat
      661 caggagcttg gaggagttgt gtctgcaagt gaccgacctg ctgccaggcc ttaggaagct
      721 caggaaccta ctccctgagc atggatgcct gctgctgtcc cctgggaact tctggcagaa
      781 tgactgggaa cgcttccatg ctgatcctga catcattggg accatccacc agcacgagcc
      841 taaaaccctg cagacttcag ccacactcaa agacttgtta tttggtgttc ctgggaagta
      901 cagcggggtg agcctctaca ccaggaagag gatggtctcc tacaccatca ccctggtctt
      961 ccagcactac catgccaagt tcctgggcag cctgcgtgcc cgcctgatgc ttctgcaccc
     1021 cagccccaac tgcagccttc gggcggagag cctggtccac gtgcacttca aggaggagat
     1081 tggtgtcgct gagctcatcc cccttgtgac cacctacatc atcttgtttg cctacatcta
     1141 cttctccacg cggaagatcg acatggtcaa gtccaagtgg gggctggccc tggctgccgt
     1201 ggtcacagtg ctcagctcgc tgctcatgtc tgtgggactc tgcacactct tcggcctgac
     1261 gcccaccctc aatggcggcg agattttccc ctaccttgtg gtggttattg ggttagagaa
     1321 tgtgttggtg ctcaccaagt ctgtggtctc aaccccggta gacctggagg tgaagctgcg
     1381 gatcgcccaa ggcctaagca gcgagagctg gtccatcatg aagaacatgg ccacggagct
     1441 gggcatcatc ctcatcggct acttcaccct agtgcccgcc atccaggagt tctgtctctt
     1501 tgctgtcgtg gggctggtgt ctgacttctt ccttcagatg ctgtttttca ccactgtcct
     1561 gtccattgac attcgccgga tggagctagc agacctgaac aagcgactgc cccctgaggc
     1621 ctgcctgccc tcagccaagc cagtgggaca gccaacgcgc tacgagcggc agctggctgt
     1681 gaggccgtcc acaccccaca ccatcacgtt gcagccgtct tccttccgaa acctgcggct
     1741 ccccaagagg ctgcgtgttg tctacttcct ggcccgcacc cgcctggcac agcgcctcat
     1801 catggctggc accgttgtct ggattggcat cctggtatac acagacccag cagggctgcg
     1861 caactacctc gctgcccagg tgacggaaca gagcccattg ggtgagggag ccctggctcc
     1921 catgcccgtg cctagtggca tgctgccccc cagccacccg gaccctgcct tctccatctt
     1981 cccacctgat gcccctaagc tacctgagaa ccagacgtcg ccaggcgagt cacctgagcg
     2041 tggaggtcca gcagaggttg tccatgacag cccagtccca gaggtaacct gggggcctga
     2101 ggatgaggaa ctttggagga aattgtcctt ccgccactgg ccgacgctct tcagctatta
     2161 caacatcaca ctggccaaga ggtacatcag cctgctgccc gtcatcccag tcacgctccg
     2221 cctgaacccg agggaggctc tggagggccg gcaccctcag gacggccgca gtgcctggcc
     2281 cccaccgggg cccatacctg ctgggcactg ggaagcagga cccaagggcc caggtggggt
     2341 gcaggcccat ggagacgtca cgctgtacaa ggtggcggcg ctgggcctgg ccaccggcat
     2401 cgtcttggtg ctgctgctgc tctgcctcta ccgcgtgcta tgcccgcgca actacgggca
     2461 gctgggtggt gggcccgggc ggcggaggcg cggggagctg ccctgcgacg actacggcta
     2521 tgcgccaccc gagacggaga tcgtgccgct tgtgctgcgc ggccacctca tggacatcga
     2581 gtgcctggcc agcgacggca tgctgctggt gagctgctgc ctggcaggcc acgtctgcgt
     2641 gtgggacgcg cagaccgggg attgcctaac gcgcattccg cgcccaggca ggcagcgccg
     2701 ggacagtggc gtgggcagcg ggcttgaggc tcaggagagc tgggaacgac tttcagatgg
     2761 tgggaaggct ggtccagagg agcctgggga cagccctccc ctgagacacc gcccccgggg
     2821 ccctccgccg ccttccctct tcggggacca gcctgacctc acctgcttaa ttgacaccaa
     2881 cttttcagcg cagcctcggt cctcacagcc cactcagccc gagccccggc accgggcggt
     2941 ctgtggccgc tctcgggact ccccaggcta tgacttcagc tgcctggtgc agcgggtgta
     3001 ccaggaggag gggctggcgg ccgtctgcac accagccctg cgcccaccct cgcctgggcc
     3061 ggtgctgtcc caggcccctg aggacgaggg tggctccccc gagaaaggct ccccttccct
     3121 cgcctgggcc cccagtgccg agggttccat ctggagcttg gagctgcagg gcaacctcat
     3181 cgtggtgggg cggagcagcg gccggctgga ggtgtgggac gccattgaag gggtgctgtg
     3241 ctgcagcagc gaggaggtct cctcaggcat taccgctctg gtgttcttgg acaaaaggat
     3301 tgtggctgca cggctcaacg gttcccttga tttcttctcc ttggagaccc acactgccct
     3361 cagccccctg cagtttagag ggaccccagg gcggggcagt tcccctgcct ctccagtgta
     3421 cagcagcagc gacacagtgg cctgtcacct gacccacaca gtgccctgtg cacaccaaaa
     3481 acccatcaca gccctgaaag ccgctgctgg gcgcttggtg actgggagcc aagaccacac
     3541 actgagagtg ttccgtctgg aggactcgtg ctgcctcttc acccttcagg gccactcagg
     3601 ggccatcacg accgtgtaca ttgaccagac catggtgctg gccagtggag gacaagatgg
     3661 ggccatctgc ctgtgggatg tactgactgg cagccgggtc agccatgtgt ttgctcaccg
     3721 tggggatgtc acctccctta cctgtaccac ctcctgtgtc atcagcagtg gcctggatga
     3781 cctcatcagc atctgggacc gcagcacagg catcaagttc tactccattc agcaggacct
     3841 gggctgtggt gcaagcttgg gtgtcatctc agacaacctg ctggtgactg gcggccaggg
     3901 ctgtgtctcc ttttgggacc taaactacgg ggacctgtta cagacagtct acctggggaa
     3961 gaacagtgag gcccagcctg cccgccagat cctggtgctg gacaacgctg ccattgtctg
     4021 caactttggc agtgagctca gcctggtgta tgtgccctct gtgctggaga agctggactg
     4081 agcgcagggc ctccttgccc aggcaggagg ctggggtgct gtgtgggggc caatgcactg
     4141 aacctggact tgggggaaag agccgagtat cttccagccg ctgcctcctg actgtaataa
     4201 tattaaactt ttttaaaaaa ccatatcatc atctgtcagg cactttggga gcta
//
