LOCUS       NM_006918               6854 bp    mRNA    linear   PRI 08-OCT-2024
DEFINITION  Homo sapiens sterol-C5-desaturase (SC5D), transcript variant 1,
            mRNA.
ACCESSION   NM_006918
VERSION     NM_006918.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6854)
  AUTHORS   Li,Y., Ran,Q., Duan,Q., Jin,J., Wang,Y., Yu,L., Wang,C., Zhu,Z.,
            Chen,X., Weng,L., Li,Z., Wang,J., Wu,Q., Wang,H., Tian,H., Song,S.,
            Shan,Z., Zhai,Q., Qin,H., Chen,S., Fang,L., Yin,H., Zhou,H.,
            Jiang,X. and Wang,P.
  TITLE     7-Dehydrocholesterol dictates ferroptosis sensitivity
  JOURNAL   Nature 626 (7998), 411-418 (2024)
   PUBMED   38297130
REFERENCE   2  (bases 1 to 6854)
  AUTHORS   Freitas,F.P., Alborzinia,H., Dos Santos,A.F., Nepachalovich,P.,
            Pedrera,L., Zilka,O., Inague,A., Klein,C., Aroua,N., Kaushal,K.,
            Kast,B., Lorenz,S.M., Kunz,V., Nehring,H., Xavier da Silva,T.N.,
            Chen,Z., Atici,S., Doll,S.G., Schaefer,E.L., Ekpo,I., Schmitz,W.,
            Horling,A., Imming,P., Miyamoto,S., Wehman,A.M.,
            Genaro-Mattos,T.C., Mirnics,K., Kumar,L., Klein-Seetharaman,J.,
            Meierjohann,S., Weigand,I., Kroiss,M., Bornkamm,G.W., Gomes,F.,
            Netto,L.E.S., Sathian,M.B., Konrad,D.B., Covey,D.F., Michalke,B.,
            Bommert,K., Bargou,R.C., Garcia-Saez,A., Pratt,D.A., Fedorova,M.,
            Trumpp,A., Conrad,M. and Friedmann Angeli,J.P.
  TITLE     7-Dehydrocholesterol is an endogenous suppressor of ferroptosis
  JOURNAL   Nature 626 (7998), 401-410 (2024)
   PUBMED   38297129
REFERENCE   3  (bases 1 to 6854)
  AUTHORS   Pickens,C.A., Matsuo,K.H. and Fenton,J.I.
  TITLE     Relationship between Body Mass Index, C-Peptide, and
            Delta-5-Desaturase Enzyme Activity Estimates in Adult Males
  JOURNAL   PLoS One 11 (3), e0149305 (2016)
   PUBMED   27023786
  REMARK    GeneRIF: The relationship between C-peptide concentrations and D5D
            enzyme activity estimates remained significant after adjusting for
            body mass index, waist circumference, and TNF-alpha.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6854)
  AUTHORS   Jugessur,A., Shi,M., Gjessing,H.K., Lie,R.T., Wilcox,A.J.,
            Weinberg,C.R., Christensen,K., Boyles,A.L., Daack-Hirsch,S.,
            Nguyen,T.T., Christiansen,L., Lidral,A.C. and Murray,J.C.
  TITLE     Maternal genes and facial clefts in offspring: a comprehensive
            search for genetic associations in two population-based cleft
            studies from Scandinavia
  JOURNAL   PLoS One 5 (7), e11493 (2010)
   PUBMED   20634891
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6854)
  AUTHORS   Liu,B.C., Zhang,J., Wang,L., Li,X.W., Wang,Y., Ji,J., Yang,F.P.,
            Wan,C.L., Gao,L.H., Xu,Y.F., Feng,G.Y., He,L., Zhao,X.Z. and He,G.
  TITLE     No association between EGR gene family polymorphisms and
            schizophrenia in the Chinese population
  JOURNAL   Prog Neuropsychopharmacol Biol Psychiatry 34 (3), 506-509 (2010)
   PUBMED   20144677
  REMARK    GeneRIF: The EGR3 may not play a major role in schizophrenia
            susceptibility in the Chinese Han population.
REFERENCE   6  (bases 1 to 6854)
  AUTHORS   Shefer,S., Salen,G., Batta,A.K., Honda,A., Tint,G.S., Irons,M.,
            Elias,E.R., Chen,T.C. and Holick,M.F.
  TITLE     Markedly inhibited 7-dehydrocholesterol-delta 7-reductase activity
            in liver microsomes from Smith-Lemli-Opitz homozygotes
  JOURNAL   J Clin Invest 96 (4), 1779-1785 (1995)
   PUBMED   7560069
REFERENCE   7  (bases 1 to 6854)
  AUTHORS   Ves-Losada,A. and Brenner,R.R.
  TITLE     Fatty acid delta 5 desaturation in rat liver cell nuclei
  JOURNAL   Mol Cell Biochem 142 (2), 163-170 (1995)
   PUBMED   7770068
REFERENCE   8  (bases 1 to 6854)
  AUTHORS   Takakuwa,Y., Nishino,H., Ishibe,Y. and Ishibashi,T.
  TITLE     Properties and kinetics of membrane-bound enzymes when both the
            enzyme and substrate are components of the same microsomal
            membrane. Studies on lathosterol 5-desaturase
  JOURNAL   J Biol Chem 269 (45), 27889-27893 (1994)
   PUBMED   7961720
  REMARK    Erratum:[J Biol Chem 1995 Feb 24;270(8):4180]
REFERENCE   9  (bases 1 to 6854)
  AUTHORS   Poisson,J.P., Dupuy,R.P., Sarda,P., Descomps,B., Narce,M., Rieu,D.
            and Crastes de Paulet,A.
  TITLE     Evidence that liver microsomes of human neonates desaturate
            essential fatty acids
  JOURNAL   Biochim Biophys Acta 1167 (2), 109-113 (1993)
   PUBMED   8466936
REFERENCE   10 (bases 1 to 6854)
  AUTHORS   Prasun,P. and Ganesh,J.
  TITLE     Lathosterolosis
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   38060690
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AP002959.2 and AK223141.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_006918.4.
            
            Summary: This gene encodes an enzyme of cholesterol biosynthesis.
            The encoded protein catalyzes the conversion of lathosterol into
            7-dehydrocholesterol. Mutations in this gene have been associated
            with lathosterolosis. Alternatively spliced transcript variants
            encoding the same protein have been described. [provided by RefSeq,
            Jul 2008].
            
            Transcript Variant: This variant (1) represents the shorter
            transcript. Variants 1 and 2 encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803615.14790.1,
                                           SRR1660803.152143.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000264027.9/ ENSP00000264027.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-7                 AP002959.2         34941-34947
            8-1036              AK223141.1         1-1029
            1037-6854           AP002959.2         49763-55580
FEATURES             Location/Qualifiers
     source          1..6854
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q23.3-q24.1"
     gene            1..6854
                     /gene="SC5D"
                     /gene_synonym="ERG3; S5DES; SC5DL"
                     /note="sterol-C5-desaturase"
                     /db_xref="GeneID:6309"
                     /db_xref="HGNC:HGNC:10547"
                     /db_xref="MIM:602286"
     exon            1..46
                     /gene="SC5D"
                     /gene_synonym="ERG3; S5DES; SC5DL"
                     /inference="alignment:Splign:2.1.0"
     exon            47..266
                     /gene="SC5D"
                     /gene_synonym="ERG3; S5DES; SC5DL"
                     /inference="alignment:Splign:2.1.0"
     CDS             57..956
                     /gene="SC5D"
                     /gene_synonym="ERG3; S5DES; SC5DL"
                     /EC_number="1.14.19.20"
                     /note="lathosterol dehydrogenase; fungal ERG3,
                     delta-5-desaturase-like;
                     3beta-hydroxysteroid-delta5-desaturase; lathosterol
                     5-desaturase; C-5 sterol desaturase; delta(7)-sterol
                     5-desaturase; sterol-C5-desaturase (ERG3
                     delta-5-desaturase homolog, S. cerevisiae)-like;
                     delta(7)-sterol C5(6)-desaturase; delta(7)-sterol
                     5(6)-desaturase"
                     /codon_start=1
                     /product="lathosterol oxidase"
                     /protein_id="NP_008849.2"
                     /db_xref="CCDS:CCDS8435.1"
                     /db_xref="GeneID:6309"
                     /db_xref="HGNC:HGNC:10547"
                     /db_xref="MIM:602286"
                     /translation="MDLVLRVADYYFFTPYVYPATWPEDDIFRQAISLLIVTNVGAYI
                     LYFFCATLSYYFVFDHALMKHPQFLKNQVRREIKFTVQALPWISILTVALFLLEIRGY
                     SKLHDDLGEFPYGLFELVVSIISFLFFTDMFIYWIHRGLHHRLVYKRLHKPHHIWKIP
                     TPFASHAFHPIDGFLQSLPYHIYPFIFPLHKVVYLSLYILVNIWTISIHDGDFRVPQI
                     LQPFINGSAHHTDHHMFFDYNYGQYFTLWDRIGGSFKNPSSFEGKGPLSYVKEMTEGK
                     RSSHSGNGCKNEKLFNGEFTKTE"
     exon            267..399
                     /gene="SC5D"
                     /gene_synonym="ERG3; S5DES; SC5DL"
                     /inference="alignment:Splign:2.1.0"
     exon            400..500
                     /gene="SC5D"
                     /gene_synonym="ERG3; S5DES; SC5DL"
                     /inference="alignment:Splign:2.1.0"
     exon            501..6854
                     /gene="SC5D"
                     /gene_synonym="ERG3; S5DES; SC5DL"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1451..1456
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SC5D"
                     /gene_synonym="ERG3; S5DES; SC5DL"
                     /note="hexamer: AATAAA"
     polyA_site      1480
                     /gene="SC5D"
                     /gene_synonym="ERG3; S5DES; SC5DL"
                     /note="major polyA site"
     regulatory      2055..2060
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SC5D"
                     /gene_synonym="ERG3; S5DES; SC5DL"
                     /note="hexamer: AATAAA"
     polyA_site      2082
                     /gene="SC5D"
                     /gene_synonym="ERG3; S5DES; SC5DL"
                     /note="major polyA site"
     regulatory      6825..6830
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SC5D"
                     /gene_synonym="ERG3; S5DES; SC5DL"
                     /note="hexamer: AATAAA"
     polyA_site      6854
                     /gene="SC5D"
                     /gene_synonym="ERG3; S5DES; SC5DL"
ORIGIN      
        1 agtttgcaga gcagtggcgt gcggagcggc ggcggaccac ctccaggggc taagtgatgg
       61 atcttgtact ccgtgttgca gattactatt tttttacacc atacgtgtat ccagccacat
      121 ggccagaaga tgacatcttc cgacaagcta ttagtcttct gattgtaaca aatgttggtg
      181 cttacatcct ttatttcttc tgtgcaacac tgagctatta ttttgtcttc gatcatgcat
      241 taatgaaaca tccacaattt ttaaagaatc aagtccgtcg agagattaag tttactgtcc
      301 aggcattgcc atggataagt attcttactg ttgcactgtt cttgctggag ataagaggtt
      361 acagcaaatt acatgatgac ctaggagagt ttccatatgg attgtttgaa cttgtcgtta
      421 gtataatatc tttcctcttt ttcactgaca tgttcatcta ctggattcac agaggccttc
      481 atcatagact ggtatataag cgcctacata aacctcacca tatttggaag attcctactc
      541 catttgcaag tcatgctttt caccctattg atggctttct tcagagtcta ccttaccata
      601 tatacccttt tatctttcca ttacacaagg tggtttattt aagtctgtac atcttggtta
      661 atatctggac aatttccatt catgacggtg attttcgtgt cccccaaatc ttacagccat
      721 ttattaatgg ctcagctcat catacagacc accatatgtt ctttgactat aattatggac
      781 aatatttcac tttgtgggat aggattggcg gctcattcaa aaatccttca tcctttgagg
      841 ggaagggacc gctcagttat gtgaaggaga tgacagaggg aaagcgcagc agccattcag
      901 gaaatggctg taagaatgaa aaattattca atggagagtt tacaaagact gaatagatta
      961 ttgcccagtt attcttaagt aaggacaaag aaggaaatat catcgtattt ctttttttta
     1021 ataaggaaaa aataatatcc atacagtcaa gatacatagt aaatggtatc atttggaaat
     1081 cagcatcgtg ggcactgctg aggaatgatc ctagtggtag gtcagaagaa gatgctgtga
     1141 acaccaggac tttaatctta tgcttaaaat gccagatgtt gttcggggga caacttgtat
     1201 ctttctagca gcagatctgt agtttgtata gcctcaacaa caattttaaa taagatggag
     1261 aataaattat tgaggggact aggctatatg catttgcctt catccaccca tgtttattaa
     1321 gaatcattgt gcttaataat accaagacta agcaccataa ccaagaaata ctaatgtaaa
     1381 gattgtttct tgtttcagga atggttaatt cttcaacgtt ggtatgataa tgataacttg
     1441 ttttgacttg aataaagtac tacatcagtg tggaaaaaaa ttctgataca ttagcagcta
     1501 tgtaaatgac ctaattgata gcaggtgtaa taagactatc gtcttcctac acataggagg
     1561 ctcattctct ggacacacta tcacctatta cattttactg attaacaaat aaattggaat
     1621 ttaaaaatat cgatatcacc atgatttaat ccagatctgg gattatgtag ctaaacattg
     1681 tgatgattat tatttaaaac cattatttaa taagagtaaa aatatgtgaa tctggatata
     1741 tttaaaaaaa gaaatttgat gcccagataa tatattaggc actactgatt ttttagttaa
     1801 attgatgcac tacacttttg atgtttgaag ttacaaacct gtaatttttt tgtaaaggaa
     1861 ataattgcca aatacctagg cccattgctg acgattagtt ctaaaatctt attcctcctc
     1921 ttctcccctc acttttccct acttcctctg caaaaagatt taacaaatac attcataagg
     1981 aaatgtgtgt tgtaacaaat atattgcaaa aacatagttt gtaaaggcat tctataagct
     2041 atttatgtaa aatcaataaa agttgatcat aattaaactg tatcagttga gtattatagc
     2101 agcacaaagt attctttgta cagattttgt gccaatttga agccacagaa atgatgtgga
     2161 ttgttaattg tgttttagaa catccccgga cactcagtgt cacaggggga aagaagtggg
     2221 taccacattc tgtttatatt tcacatttta actagatttg agtgttttta gcaagaaatc
     2281 agtcttaaaa tctaatgtct gggatccaga agaaaatgtc tttaatctgt gagttattgt
     2341 cacaatgtca tcttatttaa atgtaccaat tagcattttg taataggcaa atgtcattta
     2401 gtgcttttca ccaatcccac tcacccccgg tgctccgcct tgcctaagaa aaagaaatta
     2461 aggagaagta aactttattt cctaatataa tgtcagctga tatttattga gcttttcctc
     2521 tttgcccaga gactaggacc caaagaagtt aagtaactat tcccaggttt atttctctct
     2581 catatgatgt cccatgtgga tgtttgtggt cagtggacag ctttccacct agtctttctg
     2641 cgacccaggc tccttcctct tggggctctg cctttctctc agtccataga gccctctttg
     2701 ttgaaagagc acataggaaa agaaggaaaa gtctgtgtgg aaaatgtttc tgggtcaggc
     2761 ctggaagtgg tgcatatctc ttccgcccat gttcctttgg acagaactcc gtcacatggc
     2821 ccacctagag agattttggg aaatgtgtcc agctgtgtgc ctgggaggaa gggggcacca
     2881 ttttcttgag cagctagaca gtttgccgta tttgtggtgt tctcctcttg ttgatgttga
     2941 aatggtgaat gagccataaa gtatttcagg ttatccacac actaatcatc tcagtgtctt
     3001 taattcttaa ctccaatatg aatgtttaaa gcttcctcta gattcttatt cctatataac
     3061 taatagagaa gaaaggacag cttcctatgg ggaagacaga ggcttcctca tagatgttag
     3121 gaataatcaa acttgcccct gccctttcac ccgtctcaaa ttctggtctt ttaaagcagc
     3181 gttatgttaa gtagtcctaa cattgtaata tacagtactg ccacattctc ctactttcta
     3241 ttagaggaag tcagagaata tttatggaag tgaggaccca aattaccttc tacagatgac
     3301 ttttatagtt acaggacaga aagtgaaaat caaggttacg ttttctactt ttgtggtaga
     3361 aattgagaag tgggtggata tggttcgaga agacctttca gaaacacaga gactgagtct
     3421 ttgtcttcca tgctgtctct gcagtactga gtgaatttcc ttataccctt gtatcatgtt
     3481 ttcctcccat cttctagaag ctggggacag atttggaaga gaattacaca agttcagttt
     3541 tttgatacat ggattttaca gtgcatgcag gttatttatg tagagaggag gtctgggaga
     3601 aagatggaaa ctagggagat gactgagaac aaagatattt gggattaaca cagatagaag
     3661 aaaagtttga aaccatgaga ttgtcacagc atgaaaaaag atattcaaag acactaacca
     3721 acttgagggg tgcagtggtc tgaatgtgtc ctccaaaatt caaatgtgga aatgtatttg
     3781 ccagtgagat agtatgtaga ggtggggcct tgaggaagtg attaagtcat gagggctctg
     3841 ggattaatga cttaaaaaga ggtgtgaggc agctgttcag cccttctgtc tcctgtccct
     3901 tttttttttt ttttttttga gatggagtct tgctctgttg ccccaggctg gagtgcagtg
     3961 gcgtgatctt ggttcactgc aagctccacc tcccgggttc acgccattct cctgcctcag
     4021 cctcccgagt agctgggact acaggtgccc gccaccacca cgcccggcta attttttata
     4081 tttttagtag agatggggtt tcaccgtgtt agccaggatg gtcttgatct cctgacctca
     4141 tgatctgcct gcctcggcct cccaaagtgc tgggattata ggtgtgagcc accatgccca
     4201 gccccttctg tcccttctaa cgtgaggacc cagcaacaag gtaccatctt gaaagcagca
     4261 actggggact cagcagacac caaaccaact ggtgcttaac cgtgacttcc cagcctccag
     4321 agctgtaaga aaataaattt ctattattta tagattaccc agttgaagat actttgttaa
     4381 ggcagtacaa atggactaag acaaggggga agaaatttgc attcctgatc ttcccaactt
     4441 cttcaaattc acaacacttg aatgacagtc ttatttcagc acttactgct atcacctatt
     4501 acttttatgt gtgtcttacc tatttgagat gccagattcc ttggaagtag agaccgtgtc
     4561 tgaatcatca ttgtattaaa ccactcatcc ttaacaaatg cccaagccat ggttaaagtt
     4621 caataaatac tttgttgaat ttattaataa aatggcagaa atgtcattct cttccatata
     4681 tgtttaataa atccctgata ggtgctaagc actgcactag gtaaaaattc tcttctgatg
     4741 ctgtcttttt ggccaaccat ttttttatca tttattcatt agctgacatt tgctaagtgc
     4801 tttggagggg tcaaaagggg aagtaatgag aaatcaaaga tggtccctac atcaaggata
     4861 aactatcttt ttttagtcac tcaaagtcat aaccctttgg aaacaaaacc caccagtacc
     4921 ccagattttg accacagatg aatcagtact acaaggactg gttagagggt tgaatgaatc
     4981 tgtatactca gcacttaaca cagcactctg ggtaaaagaa aaaagatcct caaagatatt
     5041 agttggttac atcaagaaag gacaaactta ggttaatcta taacttcatc tcagaggaac
     5101 aggaactttg gagataaaca gggctctgcc acttgcaagt tgcaccatcc ctggtttctc
     5161 catctgtaaa ttgattaaaa cactgcctat ctaataagat taaataagtt agaagcattc
     5221 agttaaatgt caactgaaac tattgttcat gtaaattgtg cttgatgctt tttctttcta
     5281 gattcaatga ttattgtcat tttacctcca taggccctca atagaaatca gttgcagagg
     5341 gcagaagcct agatattttc accttaaaat tggagggtga aagacattga ggtgaagtag
     5401 agatagaggg tacacagaaa aatcgtataa gtaaaactaa catcgttaac attatttact
     5461 gtaagttatc tttgtaagag tggtaaaata cattgtgttg ttaaataatt tcatttaaaa
     5521 aatgcatcac tttgtgtgtt tttatattgc taaaaccata aggccagtct acaaggtttg
     5581 tagataaaat agaaacatac cttccttgaa aagcagaata aattttttaa aggcaggaag
     5641 gaagtgtttg aaccatgtgt caacaagctt tactgtcaaa gcaggctttt ggtatgggaa
     5701 gaaaaatact tataaatact tgttttaata tttgctttat taaaatacat ttaaaataca
     5761 gcatttttaa atctctaagc tcaacttgaa gatataagaa cagtaaattt gataaaaatg
     5821 agaaattaca ttcccatttc tttaacaatt tgtaaattcc aattatcctg aacatttaat
     5881 accatttaca tattttatta atcacatttt cttaaacatt tgataagaga tttaatattt
     5941 tgatccaact accaaaaaag cagacttgtg tacttgacag atttttctaa acacttcaca
     6001 actcacgatt caaacaaaga caaaatagca tatcaaaagt taatcactca gttggaaagc
     6061 actcatacca taggctttta ttcatttctt gaataatttt gttatatctt cctcttttag
     6121 gctgcaatga gctataattg cactactgca ctccacgctg ggtgacagag caagacccta
     6181 tctctaaaaa taaaaaagta tatatatata aaaatatctt cctctattat aatttaactc
     6241 attaagccat ttatttagat gtaaacttgc ccccctgaca tgtggtatga aacaaataga
     6301 aacctagaaa tttagtgcat attcaaatat taagacagac actggtgtgg tgacttttgt
     6361 ctgtcgcttc attgggacgt tttttctttc tgatcaactt aatgaaatta taatttacta
     6421 taattaagtg tagccatttt tactgtagag ttcaatgatc tttgatgaac gtgtacaccc
     6481 atgtaaccac cacccccaat caaagtaaag aacattttct taccagaata aatttcctct
     6541 ccgtttgcag tcattctccc cagccctagg tcaccactga tccaccttct gttactggaa
     6601 ggttagtttt cttccctgat ttagaatttc atataaatta aatcagatag tatatactct
     6661 tgtgtttagt ttctttagct taacatgttt agagatattt gctgttgcct gtgtctgtag
     6721 ctttttgttt tcattgctga atagtatttc attgtaatat accacagttg gtttatgtat
     6781 ttgctgatga atatttgtgt tatttccagc gtgggattat tatgaataaa gttgctacaa
     6841 acatttgtat acaa
//
