LOCUS       NM_002972               8019 bp    mRNA    linear   PRI 08-OCT-2024
DEFINITION  Homo sapiens SET binding factor 1 (SBF1), transcript variant 1,
            mRNA.
ACCESSION   NM_002972 NM_197971 XM_037447
VERSION     NM_002972.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8019)
  AUTHORS   Khamse,S., Alizadeh,S., Bernhart,S.H., Afshar,H., Delbari,A. and
            Ohadi,M.
  TITLE     A (GCC) repeat in SBF1 reveals a novel biological phenomenon in
            human and links to late onset neurocognitive disorder
  JOURNAL   Sci Rep 12 (1), 15480 (2022)
   PUBMED   36104480
  REMARK    GeneRIF: A (GCC) repeat in SBF1 reveals a novel biological
            phenomenon in human and links to late onset neurocognitive
            disorder.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8019)
  AUTHORS   Gang,Q., Bettencourt,C., Holton,J., Lovejoy,C., Chelban,V.,
            Oconnor,E., Yuan,Y., Reilly,M.M., Hanna,M. and Houlden,H.
  TITLE     A novel frameshift deletion in autosomal recessive SBF1-related
            syndromic neuropathy with necklace fibres
  JOURNAL   J Neurol 267 (9), 2705-2712 (2020)
   PUBMED   32444983
  REMARK    GeneRIF: A novel frameshift deletion in autosomal recessive
            SBF1-related syndromic neuropathy with necklace fibres.
REFERENCE   3  (bases 1 to 8019)
  AUTHORS   Flusser,H., Halperin,D., Kadir,R., Shorer,Z., Shelef,I. and
            Birk,O.S.
  TITLE     Novel SBF1 splice-site null mutation broadens the clinical spectrum
            of Charcot-Marie-Tooth type 4B3 disease
  JOURNAL   Clin Genet 94 (5), 473-479 (2018)
   PUBMED   30039846
  REMARK    GeneRIF: The novel SBF1 null mutation highlights distinct severe
            phenotypic manifestations, broadening the clinical spectrum of
            SBF1-related neuropathies: cerebellar and pyramidal signs evident
            in the first months of life with peripheral polyneuropathy emerging
            only toward the end of the first decade, together with unique MRI
            findings.
REFERENCE   4  (bases 1 to 8019)
  AUTHORS   Manole,A., Horga,A., Gamez,J., Raguer,N., Salvado,M., San
            Millan,B., Navarro,C., Pittmann,A., Reilly,M.M. and Houlden,H.
  TITLE     SBF1 mutations associated with autosomal recessive axonal
            neuropathy with cranial nerve involvement
  JOURNAL   Neurogenetics 18 (1), 63-67 (2017)
   PUBMED   28005197
  REMARK    GeneRIF: Novel sequence variants in SBF1 (c.1168C>G and
            c.2209_2210del) as the potential causative mutations in two
            siblings with severe axonal neuropathy, hearing loss, facial
            weakness and bulbar features.
REFERENCE   5  (bases 1 to 8019)
  AUTHORS   Zhu,M.H., Ji,S.L., Zhang,C.Y., Cui,L., Xiong,L. and Zheng,H.L.
  TITLE     DNA microarray reveals ZNF195 and SBF1 are potential biomarkers for
            gemcitabine sensitivity in head and neck squamous cell carcinoma
            cell lines
  JOURNAL   Int J Clin Exp Pathol 7 (4), 1514-1523 (2014)
   PUBMED   24817947
  REMARK    GeneRIF: ZNF195 and SBF1 are potential biomarkers for gemcitabine
            sensitivity in head and neck squamous cell carcinoma cell lines.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 8019)
  AUTHORS   Firestein,R. and Cleary,M.L.
  TITLE     Pseudo-phosphatase Sbf1 contains an N-terminal GEF homology domain
            that modulates its growth regulatory properties
  JOURNAL   J Cell Sci 114 (Pt 16), 2921-2927 (2001)
   PUBMED   11686296
REFERENCE   7  (bases 1 to 8019)
  AUTHORS   Firestein,R., Cui,X., Huie,P. and Cleary,M.L.
  TITLE     Set domain-dependent regulation of transcriptional silencing and
            growth control by SUV39H1, a mammalian ortholog of Drosophila
            Su(var)3-9
  JOURNAL   Mol Cell Biol 20 (13), 4900-4909 (2000)
   PUBMED   10848615
REFERENCE   8  (bases 1 to 8019)
  AUTHORS   Laporte,J., Blondeau,F., Buj-Bello,A., Tentler,D., Kretz,C.,
            Dahl,N. and Mandel,J.L.
  TITLE     Characterization of the myotubularin dual specificity phosphatase
            gene family from yeast to human
  JOURNAL   Hum Mol Genet 7 (11), 1703-1712 (1998)
   PUBMED   9736772
  REMARK    GeneRIF: SBF1 (MTMR5) belongs to the myotubularin family of
            phosphoinositides phosphatases-like
REFERENCE   9  (bases 1 to 8019)
  AUTHORS   Cui,X., De Vivo,I., Slany,R., Miyamoto,A., Firestein,R. and
            Cleary,M.L.
  TITLE     Association of SET domain and myotubularin-related proteins
            modulates growth control
  JOURNAL   Nat Genet 18 (4), 331-337 (1998)
   PUBMED   9537414
REFERENCE   10 (bases 1 to 8019)
  AUTHORS   Bird,T.D.
  TITLE     Charcot-Marie-Tooth Neuropathy Type 4 - RETIRED CHAPTER, FOR
            HISTORICAL REFERENCE ONLY
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301641
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from HY035781.1, BC087612.1,
            AL096767.14 and BM975530.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_002972.3.
            
            Summary: This gene encodes a member of the protein-tyrosine
            phosphatase family. However, the encoded protein does not appear to
            be a catalytically active phosphatase because it lacks several
            amino acids in the catalytic pocket. This protein contains a
            Guanine nucleotide exchange factor (GEF) domain which is necessary
            for its role in growth and differentiation. Mutations in this gene
            have been associated with Charcot-Marie-Tooth disease 4B3.
            Pseudogenes of this gene have been defined on chromosomes 1 and 8.
            [provided by RefSeq, Dec 2014].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC087612.1, SRR14038194.2905869.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000380817.8/ ENSP00000370196.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-25                HY035781.1         10-34
            26-6024             BC087612.1         1-5999
            6025-7720           AL096767.14        20728-22423         c
            7721-8019           BM975530.1         15-313              c
FEATURES             Location/Qualifiers
     source          1..8019
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="22"
                     /map="22q13.33"
     gene            1..8019
                     /gene="SBF1"
                     /gene_synonym="CMT4B3; DENND7A; MTMR5"
                     /note="SET binding factor 1"
                     /db_xref="GeneID:6305"
                     /db_xref="HGNC:HGNC:10542"
                     /db_xref="MIM:603560"
     exon            1..250
                     /gene="SBF1"
                     /gene_synonym="CMT4B3; DENND7A; MTMR5"
                     /inference="alignment:Splign:2.1.0"
     CDS             196..5877
                     /gene="SBF1"
                     /gene_synonym="CMT4B3; DENND7A; MTMR5"
                     /note="isoform 1 is encoded by transcript variant 1;
                     myotubularin-related protein 5; DENN/MADD domain
                     containing 7A; inactive phosphatidylinositol 3-phosphatase
                     5"
                     /codon_start=1
                     /product="myotubularin-related protein 5 isoform 1"
                     /protein_id="NP_002963.2"
                     /db_xref="CCDS:CCDS14091.2"
                     /db_xref="GeneID:6305"
                     /db_xref="HGNC:HGNC:10542"
                     /db_xref="MIM:603560"
                     /translation="MARLADYFVLVAFGPHPRGSGEGQGQILQRFPEKDWEDNPFPQG
                     IELFCQPSGWQLCPERNPPTFFVAVLTDINSERHYCACLTFWEPAEPSQETTRVEDAT
                     EREEEGDEGGQTHLSPTAPAPSAQLFAPKTLVLVSRLDHTEVFRNSLGLIYAIHVEGL
                     NVCLENVIGNLLTCTVPLAGGSQRTISLGAGDRQVIQTPLADSLPVSRCSVALLFRQL
                     GITNVLSLFCAALTEHKVLFLSRSYQRLADACRGLLALLFPLRYSFTYVPILPAQLLE
                     VLSTPTPFIIGVNAAFQAETQELLDVIVADLDGGTVTIPECVHIPPLPEPLQSQTHSV
                     LSMVLDPELELADLAFPPPTTSTSSLKMQDKELRAVFLRLFAQLLQGYRWCLHVVRIH
                     PEPVIRFHKAAFLGQRGLVEDDFLMKVLEGMAFAGFVSERGVPYRPTDLFDELVAHEV
                     ARMRADENHPQRVLRHVQELAEQLYKNENPYPAVAMHKVQRPGESSHLRRVPRPFPRL
                     DEGTVQWIVDQAAAKMQGAPPAVKAERRTTVPSGPPMTAILERCSGLHVNSARRLEVV
                     RNCISYVFEGKMLEAKKLLPAVLRALKGRAARRCLAQELHLHVQQNRAVLDHQQFDFV
                     VRMMNCCLQDCTSLDEHGIAAALLPLVTAFCRKLSPGVTQFAYSCVQEHVVWSTPQFW
                     EAMFYGDVQTHIRALYLEPTEDLAPAQEVGEAPSQEDERSALDVASEQRRLWPTLSRE
                     KQQELVQKEESTVFSQAIHYANRMSYLLLPLDSSKSRLLRERAGLGDLESASNSLVTN
                     SMAGSVAESYDTESGFEDAETCDVAGAVVRFINRFVDKVCTESGVTSDHLKGLHVMVP
                     DIVQMHIETLEAVQRESRRLPPIQKPKLLRPRLLPGEECVLDGLRVYLLPDGREEGAG
                     GSAGGPALLPAEGAVFLTTYRVIFTGMPTDPLVGEQVVVRSFPVAALTKEKRISVQTP
                     VDQLLQDGLQLRSCTFQLLKMAFDEEVGSDSAELFRKQLHKLRYPPDIRATFAFTLGS
                     AHTPGRPPRVTKDKGPSLRTLSRNLVKNAKKTIGRQHVTRKKYNPPSWEHRGQPPPED
                     QEDEISVSEELEPSTLTPSSALKPSDRMTMSSLVERACCRDYQRLGLGTLSSSLSRAK
                     SEPFRISPVNRMYAICRSYPGLLIVPQSVQDNALQRVSRCYRQNRFPVVCWRSGRSKA
                     VLLRSGGLHGKGVVGLFKAQNAPSPGQSQADSSSLEQEKYLQAVVSSMPRYADASGRN
                     TLSGFSSAHMGSHVPSPRARVTTLSNPMAASASRRTAPRGKWGSVRTSGRSSGLGTDV
                     GSRLAGRDALAPPQANGGPPDPGFLRPQRAALYILGDKAQLKGVRSDPLQQWELVPIE
                     VFEARQVKASFKKLLKACVPGCPAAEPSPASFLRSLEDSEWLIQIHKLLQVSVLVVEL
                     LDSGSSVLVGLEDGWDITTQVVSLVQLLSDPFYRTLEGFRLLVEKEWLSFGHRFSHRG
                     AHTLAGQSSGFTPVFLQFLDCVHQVHLQFPMEFEFSQFYLKFLGYHHVSRRFRTFLLD
                     SDYERIELGLLYEEKGERRGQVPCRSVWEYVDRLSKRTPVFHNYMYAPEDAEVLRPYS
                     NVSNLKVWDFYTEETLAEGPPYDWELAQGPPEPPEEERSDGGAPQSRRRVVWPCYDSC
                     PRAQPDAISRLLEELQRLETELGQPAERWKDTWDRVKAAQRLEGRPDGRGTPSSLLVS
                     TAPHHRRSLGVYLQEGPVGSTLSLSLDSDQSSGSTTSGSRQAARRSTSTLYSQFQTAE
                     SENRSYEGTLYKKGAFMKPWKARWFVLDKTKHQLRYYDHRVDTECKGVIDLAEVEAVA
                     PGTPTMGAPKTVDEKAFFDVKTTRRVYNFCAQDVPSAQQWVDRIQSCLSDA"
     exon            251..336
                     /gene="SBF1"
                     /gene_synonym="CMT4B3; DENND7A; MTMR5"
                     /inference="alignment:Splign:2.1.0"
     exon            337..474
                     /gene="SBF1"
                     /gene_synonym="CMT4B3; DENND7A; MTMR5"
                     /inference="alignment:Splign:2.1.0"
     exon            475..633
                     /gene="SBF1"
                     /gene_synonym="CMT4B3; DENND7A; MTMR5"
                     /inference="alignment:Splign:2.1.0"
     exon            634..744
                     /gene="SBF1"
                     /gene_synonym="CMT4B3; DENND7A; MTMR5"
                     /inference="alignment:Splign:2.1.0"
     exon            745..850
                     /gene="SBF1"
                     /gene_synonym="CMT4B3; DENND7A; MTMR5"
                     /inference="alignment:Splign:2.1.0"
     exon            851..983
                     /gene="SBF1"
                     /gene_synonym="CMT4B3; DENND7A; MTMR5"
                     /inference="alignment:Splign:2.1.0"
     exon            984..1092
                     /gene="SBF1"
                     /gene_synonym="CMT4B3; DENND7A; MTMR5"
                     /inference="alignment:Splign:2.1.0"
     exon            1093..1206
                     /gene="SBF1"
                     /gene_synonym="CMT4B3; DENND7A; MTMR5"
                     /inference="alignment:Splign:2.1.0"
     exon            1207..1284
                     /gene="SBF1"
                     /gene_synonym="CMT4B3; DENND7A; MTMR5"
                     /inference="alignment:Splign:2.1.0"
     exon            1285..1398
                     /gene="SBF1"
                     /gene_synonym="CMT4B3; DENND7A; MTMR5"
                     /inference="alignment:Splign:2.1.0"
     exon            1399..1527
                     /gene="SBF1"
                     /gene_synonym="CMT4B3; DENND7A; MTMR5"
                     /inference="alignment:Splign:2.1.0"
     exon            1528..1626
                     /gene="SBF1"
                     /gene_synonym="CMT4B3; DENND7A; MTMR5"
                     /inference="alignment:Splign:2.1.0"
     exon            1627..1831
                     /gene="SBF1"
                     /gene_synonym="CMT4B3; DENND7A; MTMR5"
                     /inference="alignment:Splign:2.1.0"
     exon            1832..1944
                     /gene="SBF1"
                     /gene_synonym="CMT4B3; DENND7A; MTMR5"
                     /inference="alignment:Splign:2.1.0"
     exon            1945..2094
                     /gene="SBF1"
                     /gene_synonym="CMT4B3; DENND7A; MTMR5"
                     /inference="alignment:Splign:2.1.0"
     exon            2095..2163
                     /gene="SBF1"
                     /gene_synonym="CMT4B3; DENND7A; MTMR5"
                     /inference="alignment:Splign:2.1.0"
     exon            2164..2322
                     /gene="SBF1"
                     /gene_synonym="CMT4B3; DENND7A; MTMR5"
                     /inference="alignment:Splign:2.1.0"
     exon            2323..2591
                     /gene="SBF1"
                     /gene_synonym="CMT4B3; DENND7A; MTMR5"
                     /inference="alignment:Splign:2.1.0"
     exon            2592..2764
                     /gene="SBF1"
                     /gene_synonym="CMT4B3; DENND7A; MTMR5"
                     /inference="alignment:Splign:2.1.0"
     exon            2765..2838
                     /gene="SBF1"
                     /gene_synonym="CMT4B3; DENND7A; MTMR5"
                     /inference="alignment:Splign:2.1.0"
     exon            2839..3034
                     /gene="SBF1"
                     /gene_synonym="CMT4B3; DENND7A; MTMR5"
                     /inference="alignment:Splign:2.1.0"
     exon            3035..3162
                     /gene="SBF1"
                     /gene_synonym="CMT4B3; DENND7A; MTMR5"
                     /inference="alignment:Splign:2.1.0"
     exon            3163..3341
                     /gene="SBF1"
                     /gene_synonym="CMT4B3; DENND7A; MTMR5"
                     /inference="alignment:Splign:2.1.0"
     exon            3342..3478
                     /gene="SBF1"
                     /gene_synonym="CMT4B3; DENND7A; MTMR5"
                     /inference="alignment:Splign:2.1.0"
     exon            3479..3686
                     /gene="SBF1"
                     /gene_synonym="CMT4B3; DENND7A; MTMR5"
                     /inference="alignment:Splign:2.1.0"
     exon            3687..3883
                     /gene="SBF1"
                     /gene_synonym="CMT4B3; DENND7A; MTMR5"
                     /inference="alignment:Splign:2.1.0"
     exon            3884..4021
                     /gene="SBF1"
                     /gene_synonym="CMT4B3; DENND7A; MTMR5"
                     /inference="alignment:Splign:2.1.0"
     exon            4022..4099
                     /gene="SBF1"
                     /gene_synonym="CMT4B3; DENND7A; MTMR5"
                     /inference="alignment:Splign:2.1.0"
     exon            4100..4281
                     /gene="SBF1"
                     /gene_synonym="CMT4B3; DENND7A; MTMR5"
                     /inference="alignment:Splign:2.1.0"
     exon            4282..4461
                     /gene="SBF1"
                     /gene_synonym="CMT4B3; DENND7A; MTMR5"
                     /inference="alignment:Splign:2.1.0"
     exon            4462..4563
                     /gene="SBF1"
                     /gene_synonym="CMT4B3; DENND7A; MTMR5"
                     /inference="alignment:Splign:2.1.0"
     exon            4564..4749
                     /gene="SBF1"
                     /gene_synonym="CMT4B3; DENND7A; MTMR5"
                     /inference="alignment:Splign:2.1.0"
     exon            4750..4876
                     /gene="SBF1"
                     /gene_synonym="CMT4B3; DENND7A; MTMR5"
                     /inference="alignment:Splign:2.1.0"
     exon            4877..5007
                     /gene="SBF1"
                     /gene_synonym="CMT4B3; DENND7A; MTMR5"
                     /inference="alignment:Splign:2.1.0"
     exon            5008..5238
                     /gene="SBF1"
                     /gene_synonym="CMT4B3; DENND7A; MTMR5"
                     /inference="alignment:Splign:2.1.0"
     exon            5239..5346
                     /gene="SBF1"
                     /gene_synonym="CMT4B3; DENND7A; MTMR5"
                     /inference="alignment:Splign:2.1.0"
     exon            5347..5558
                     /gene="SBF1"
                     /gene_synonym="CMT4B3; DENND7A; MTMR5"
                     /inference="alignment:Splign:2.1.0"
     exon            5559..5646
                     /gene="SBF1"
                     /gene_synonym="CMT4B3; DENND7A; MTMR5"
                     /inference="alignment:Splign:2.1.0"
     exon            5647..5778
                     /gene="SBF1"
                     /gene_synonym="CMT4B3; DENND7A; MTMR5"
                     /inference="alignment:Splign:2.1.0"
     exon            5779..8019
                     /gene="SBF1"
                     /gene_synonym="CMT4B3; DENND7A; MTMR5"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6192..6197
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SBF1"
                     /gene_synonym="CMT4B3; DENND7A; MTMR5"
                     /note="hexamer: TATAAA"
     polyA_site      6213
                     /gene="SBF1"
                     /gene_synonym="CMT4B3; DENND7A; MTMR5"
                     /note="major polyA site"
     regulatory      7996..8001
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SBF1"
                     /gene_synonym="CMT4B3; DENND7A; MTMR5"
                     /note="hexamer: ATTAAA"
     polyA_site      8019
                     /gene="SBF1"
                     /gene_synonym="CMT4B3; DENND7A; MTMR5"
ORIGIN      
        1 gggcgggccg gctggctggg aagatggcgg cgggaacctg ggccgccgcc gccgccgccg
       61 ccgccgccgc ggagcgaacc aggggtgtcc ggggtgcgcg gtccagggcc ggggccgggc
      121 catgagcgcg ccgtcctcga gtccccgagc cgcggagccc gcccgcgccc ctcgggccgc
      181 cccgcgtccc tcgccatggc gcggctcgcg gactacttcg tgctggtggc gttcgggccg
      241 cacccgcgcg ggagtgggga aggccagggc cagattctgc agcgcttccc agagaaggac
      301 tgggaggaca acccattccc ccagggcatc gagctgtttt gccagcccag cgggtggcag
      361 ctgtgtcccg agaggaatcc accgaccttc tttgttgctg tcctcaccga catcaactcc
      421 gagcgccact actgcgcctg cttgaccttc tgggagccag cggagccttc acaggaaacg
      481 acgcgcgtgg aggatgccac agagagggag gaagaggggg atgagggagg ccagacccac
      541 ctgtctccca cagcacctgc cccatctgcc cagctgtttg caccgaagac gctggtactg
      601 gtgtcgcgac tcgaccacac ggaggtgttc aggaacagcc ttggcctcat ctatgccatc
      661 cacgtggagg gcctgaatgt gtgcctggag aacgtgattg ggaacctgct gacgtgcact
      721 gtgcccctgg ctgggggctc gcagaggacg atctctttgg gggctggtga ccggcaggtc
      781 atccagactc cactggccga ctcgctgccc gtcagccgct gcagcgtggc cctgctcttc
      841 cgccagctag gcatcaccaa cgtgctgtct ttgttctgtg ccgccctcac ggagcacaag
      901 gttctcttcc tgtcccggag ctaccagcgg ctcgccgatg cctgtagggg cctcctggca
      961 ctgctgtttc ctctcagata cagcttcacc tatgtgccca tcctgccggc tcagctgctg
     1021 gaggtcctca gcacacccac gcccttcatc attggggtca acgcggcctt ccaggcagag
     1081 acccaggagc tgctcgatgt gattgttgct gatctggatg gagggacggt caccattcct
     1141 gagtgtgtgc acattccacc cttgccagag ccactgcaga gtcagacgca cagtgtgctg
     1201 agcatggtcc tggacccgga gctggagttg gctgacctcg ccttccctcc gcccacgaca
     1261 tccacctcct ccctgaagat gcaggacaag gagctgcgcg cggtcttcct gcggctgttc
     1321 gctcagctgc tgcagggcta tcgctggtgc ctgcacgtcg tgcgcatcca cccggagcct
     1381 gtcatccgct tccataaggc agccttcctg ggccagcgtg ggctggtaga ggacgatttc
     1441 ctgatgaagg tgctggaggg catggccttt gctggctttg tgtcagagcg tggggtccca
     1501 taccgcccta cggacctgtt cgatgagctg gtggcccacg aggtggcaag gatgcgggcg
     1561 gatgagaacc acccccagcg tgtcctgcgt cacgtccagg aactggcaga gcagctctac
     1621 aagaacgaga acccgtaccc agccgtggcg atgcacaagg tacagaggcc cggtgagagc
     1681 agccacctgc gacgggtgcc ccgacccttc ccccggctgg atgagggcac cgtgcagtgg
     1741 atcgtggacc aggctgcagc caagatgcag ggtgcacccc cagctgtgaa ggccgagagg
     1801 aggaccaccg tgccctcagg gccccccatg actgccatac tggagcggtg cagtgggctg
     1861 catgtcaaca gcgcccggcg gctggaggtt gtgcgcaact gcatctccta cgtgtttgag
     1921 gggaaaatgc ttgaggccaa gaagctgctc ccagccgtgt tgagggccct gaaggggcga
     1981 gctgcccgcc gctgcctcgc ccaggagctg cacctgcatg tgcagcagaa ccgtgcggtc
     2041 ctggaccacc agcagtttga ctttgtcgtc cgtatgatga actgctgcct gcaggactgc
     2101 acttctctgg acgagcatgg cattgcggcg gctctgctgc ctctggtcac agccttctgc
     2161 cggaagctga gcccgggggt gacgcagttt gcatacagct gtgtgcagga gcacgtggtg
     2221 tggagcacgc cacagttctg ggaggccatg ttctatgggg atgtgcagac tcacatccgg
     2281 gccctctacc tggagcccac ggaggacctg gcccccgccc aggaggttgg ggaggcacct
     2341 tcccaggagg acgagcgctc tgccctagac gtggcttctg agcagcggcg cttgtggcca
     2401 actctgagtc gtgagaagca gcaggagctg gtgcagaagg aggagagcac ggtgttcagc
     2461 caggccatcc actatgccaa ccgcatgagc tacctcctcc tgcccctgga cagcagcaag
     2521 agccgcctac ttcgggagcg tgccgggctg ggcgacctgg agagcgccag caacagcctg
     2581 gtcaccaaca gcatggctgg cagtgtggcc gagagctatg acacggagag cggcttcgag
     2641 gatgcagaga cctgcgacgt agctggggct gtggtccgct tcatcaaccg ctttgtggac
     2701 aaggtctgca cggagagtgg ggtcaccagc gaccacctca aggggctgca tgtcatggtg
     2761 ccagacattg tccagatgca catcgagacc ctggaggccg tgcagcggga gagccggagg
     2821 ctgccgccca tccagaagcc caagctgctg cggccgcgcc tgctgccggg tgaggagtgt
     2881 gtgctggacg gcctgcgcgt ctacctgctg ccggatgggc gtgaggaggg cgcggggggc
     2941 agtgctgggg gaccagcatt gctcccagct gagggcgccg tcttcctcac cacgtaccgg
     3001 gtcatcttca cggggatgcc cacggacccc ctggttgggg agcaggtggt ggtccgctcc
     3061 ttcccggtgg ctgcgctgac caaggagaag cgcatcagcg tccagacccc tgtggaccag
     3121 ctcctgcagg acgggctcca gctgcgctcc tgcacattcc agctgctgaa aatggccttt
     3181 gacgaggagg tggggtctga cagcgccgag ctcttccgta agcagctgca taagctgcgg
     3241 tacccgccgg acatcagggc cacctttgcg ttcaccttgg gctctgccca cacacctggc
     3301 cggccaccgc gagtcaccaa ggacaagggt ccttccctca gaaccctgtc ccggaacctg
     3361 gtcaagaacg ccaagaagac catcgggcgg cagcatgtca ctcgcaagaa gtacaacccc
     3421 cccagctggg agcaccgggg ccagccgccc cctgaggacc aggaggacga gatctcagtg
     3481 tcggaggagc tggagcccag cacgctgacc ccgtcctcag ccctgaagcc ctccgaccgc
     3541 atgaccatga gcagcctggt ggaaagggct tgctgtcgcg actaccagcg cctcggtctg
     3601 ggcaccctga gcagcagcct gagccgggcc aagtctgagc ccttccgcat ttctccggtc
     3661 aaccgcatgt atgccatctg ccgcagctac ccagggctgc tgatcgtgcc ccagagtgtc
     3721 caggacaacg ccctgcagcg cgtgtcccgc tgctaccgcc agaaccgctt ccccgtggtc
     3781 tgctggcgca gcgggcggtc caaggcggtg ctgctgcgct ctggaggcct gcatggcaaa
     3841 ggtgtcgtcg gcctcttcaa ggcccagaac gcaccttctc caggccagtc ccaggcggac
     3901 tcgagtagcc tggagcagga gaagtacctg caggctgtgg tcagctccat gccccgctac
     3961 gccgacgcgt cgggacgcaa cacgcttagc ggcttctcct cagcccacat gggcagtcac
     4021 gttcccagcc ccagagccag ggtcaccacg ctgtccaacc ccatggcggc ctcggcctcc
     4081 agacggaccg caccccgagg taagtggggc agtgtccgga ccagtggacg cagcagtggc
     4141 cttggcaccg atgtgggctc ccggctagct ggcagagacg cgctggcccc accccaggcc
     4201 aacgggggcc ctcccgaccc gggcttcctg cgtccgcagc gagcagccct ctatatcctt
     4261 ggggacaaag cccagctcaa gggtgtgcgg tcagaccccc tgcagcagtg ggagctggtg
     4321 cccattgagg tattcgaggc acggcaggtg aaggctagct tcaagaagct gctgaaagca
     4381 tgtgtcccag gctgccccgc tgctgagccc agcccagcct ccttcctgcg ctcactggag
     4441 gactcagagt ggctgatcca gatccacaag ctgctgcagg tgtctgtgct ggtggtggag
     4501 ctcctggatt caggctcctc cgtgctggtg ggcctggagg atggctggga catcaccacc
     4561 caggtggtat ccttggtgca gctgctctca gaccccttct accgcacgct ggagggcttt
     4621 cgcctgctgg tggagaagga gtggctgtcc ttcggccatc gcttcagcca ccgtggagct
     4681 cacaccctgg ccgggcagag cagcggcttc acacccgtct tcctgcagtt cctggactgc
     4741 gtacaccagg tccacctgca gttccccatg gagtttgagt tcagccagtt ctacctcaag
     4801 ttcctcggct accaccatgt gtcccgccgt ttccggacct tcctgctcga ctctgactat
     4861 gagcgcattg agctggggct gctgtatgag gagaaggggg aacgcagggg ccaggtgccg
     4921 tgcaggtctg tgtgggagta tgtggaccgg ctgagcaaga ggacgcctgt gttccacaat
     4981 tacatgtatg cgcccgagga cgcagaggtc ctgcggccct acagcaacgt gtccaacctg
     5041 aaggtgtggg acttctacac tgaggagacg ctggccgagg gccctcccta tgactgggaa
     5101 ctggcccagg ggccccctga acccccagag gaagaacggt ctgatggagg cgctccccag
     5161 agcaggcgcc gcgtggtgtg gccctgttac gacagctgcc cgcgggccca gcctgacgcc
     5221 atctcacgcc tgctggagga gctgcagagg ctggagacag agttgggcca acccgctgag
     5281 cgctggaagg acacctggga ccgggtgaag gctgcacagc gcctcgaggg ccggccagac
     5341 ggccgtggca cccctagctc cctccttgtg tccaccgcac cccaccaccg tcgctcgctg
     5401 ggtgtgtacc tgcaggaggg gcccgtgggc tccaccctga gcctcagcct ggacagcgac
     5461 cagagtagtg gctcaaccac atccggctcc cgtcaggctg cccgccgcag caccagcacc
     5521 ctgtacagcc agttccagac agcagagagt gagaacaggt cctacgaggg cactctgtac
     5581 aagaaggggg ccttcatgaa gccttggaag gcccgctggt tcgtgctgga caagaccaag
     5641 caccagctgc gctactacga ccaccgtgtg gacacagagt gcaagggtgt catcgacttg
     5701 gcggaggtgg aggctgtggc acctggcacg cccactatgg gtgcccctaa gactgtggac
     5761 gagaaggcct tctttgacgt gaagacaacg cgtcgcgttt acaacttctg tgcccaggac
     5821 gtgccctcgg cccagcagtg ggtggaccgg atccagagct gcctgtcgga cgcctgagcc
     5881 tcccagccct gcccggctgc tctgcttccg gtcgttaccg accactaggg gtgggcaggg
     5941 ccgccccggc catgtttaca gccccggccc tcgacagtat tgaggccccg agcccccagc
     6001 acttgtgtgt acagcccccg tccccgcccc gccccgcccg gccggcccta acttattttg
     6061 gcgtcacagc tgagcaccgt gccgggaggt ggccaaggta cagcccgcaa tgggcctgta
     6121 aatagtccgg ccccgtcagc gtgtgctggt ccagccagcg gctgcaggcg agtttctaga
     6181 accagagtct atataaagag agaactaacg ccacgctcct gtgcctgcct tccccactcc
     6241 ccggctgcct gctctcggcc tacccagagg gtcccatctg cccctatcca ggcccacctg
     6301 gcgggaggtt ggcatctttc tcgtgagcct ctcctggtgc ctgggtccac ccagctcggc
     6361 ctgcatgtcc ctgggagtga ctttgctctg ggggcggatc gagcaggagg cttcactggg
     6421 gacttgcttg attccctcca cgcctcaggg ctggtctagg ggccggcacg gctggagagg
     6481 aagcccccat ccctacccag gggatgcaga agctgacctc acagaggctt gggggtgaaa
     6541 gggtgggtgg tcatttgacc ccagaaggct gttgcaggtc cagaggacac ttgaggtgga
     6601 cgtcagtttc tggctagacc cgagctgaag ggatggaggc cggaggcggg gggggggggg
     6661 ggacagtggg ctcccagggg aatgcaggtt gaccacatct ggctcctgcc aggcaacgag
     6721 cagcatctgg cagagtaagg ggccaacgcc catgggggat ggaccctctc agttcttggg
     6781 aattctgccc caaaagtcct ttccctgggg tctcagaggg cccccgtcct tcccttcttg
     6841 gtgtcactgt ggcccctcac tgctcttttc ctattcaaac ctgagtccca ccaggcccag
     6901 ggcttcacct gctgagctgt tgtgtccttg cctgtgacga ggcctggcca ggggtgcagg
     6961 agcagaaggt ggggagggtt atagacgctg caaaggccaa gagaacatct gagagtggca
     7021 gctggtgacc tggccagagg ggctggtgag gggcagagaa cctggctaga ggctgggtcc
     7081 ctcaggtggt cctctcaggt gggaggcgag cagcaggtgt gggtgagggg aaggttctga
     7141 tgacagctgc agaggcaggg cccagtgctg gcaggtgggg ggccaagacc ctcccctggt
     7201 gggacgttga agccaaggat ggccttggac cctgtcaggc ccagcatggt cccgccacct
     7261 cccccacccc acaggtggtg ttgggacacc tgggcgagat gtgagggtgg gctcacttga
     7321 gccactgaaa ccagccaggt cttccctcag gccggacaga tggcgcctga ccgaagttcc
     7381 tggcacctgg aaaacccaca ggtcagagta aggggagaaa ggaccctgcc ctccctgttc
     7441 cacgtctgtg gggggagagg acaaatgcca ggcacagggt aggcggcgag aacaaggcac
     7501 tcaatgtgta gctggggcag agactcggcc tctggggagc tgagcgggtt ccctccaccc
     7561 ccaaccgtgg tggaaagaca agctcgctgg ggcggggtgg gggtctggtc tccacctgcc
     7621 cctcccactc agccactgag gacaaggtgg ggcccaggct tctgggaggg ggagctggca
     7681 caaaaggaag tcctggggtt gatgtgtttg agcgttaggc gaagtggttc cccccatccc
     7741 ccaaacggaa aaatgtcagt atttgctaag ctgtagagac ctgatgccgt gatgtggcct
     7801 gttccgcctc cacccattac acggggataa cgctgggggg tggcgggccc acaaaagagg
     7861 tgctggagga gactctccca cccctggccg ggccggggct ttggggccgg aaggttcaca
     7921 gtacgcggtt tgtccgaacg tcacggcttt tattgggagt tgggggtttg gggtgccctg
     7981 tcaggtgatc agaacattaa aaatggactc aacgtaaaa
//
