LOCUS       NM_016127               2020 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens store-operated calcium entry associated regulatory
            factor (SARAF), transcript variant 1, mRNA.
ACCESSION   NM_016127
VERSION     NM_016127.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Dai,Y., Xu,Y., Shen,J., Hu,C., Li,X., Chen,Y., Liu,Y. and Hu,D.
  TITLE     MiR-30a-5p isoform -1|1 promotes the progression of gastric cancer
            by inhibiting TMEM66 and reducing intratumoral cytotoxic T cells
  JOURNAL   Exp Cell Res 439 (2), 114099 (2024)
   PUBMED   38802035
  REMARK    GeneRIF: MiR-30a-5p isoform -1|1 promotes the progression of
            gastric cancer by inhibiting TMEM66 and reducing intratumoral
            cytotoxic T cells.
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Rosado,J.A.
  TITLE     Reduced Ca2+ mobilization in neonatal human platelets involves
            SARAF and pannexin-1
  JOURNAL   Br J Haematol 204 (3), 755-756 (2024)
   PUBMED   38073055
  REMARK    GeneRIF: Reduced Ca[2+] mobilization in neonatal human platelets
            involves SARAF and pannexin-1.
REFERENCE   3  (bases 1 to 2020)
  AUTHORS   Zomot,E., Achildiev Cohen,H., Dagan,I., Militsin,R. and Palty,R.
  TITLE     Bidirectional regulation of calcium release-activated calcium
            (CRAC) channel by SARAF
  JOURNAL   J Cell Biol 220 (12) (2021)
   PUBMED   34705029
  REMARK    GeneRIF: Bidirectional regulation of calcium release-activated
            calcium (CRAC) channel by SARAF.
REFERENCE   4  (bases 1 to 2020)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 2020)
  AUTHORS   Uslu,M., Albayrak,E. and Kocabas,F.
  TITLE     Temporal modulation of calcium sensing in hematopoietic stem cells
            is crucial for proper stem cell expansion and engraftment
  JOURNAL   J Cell Physiol 235 (12), 9644-9666 (2020)
   PUBMED   32394484
  REMARK    GeneRIF: Temporal modulation of calcium sensing in hematopoietic
            stem cells is crucial for proper stem cell expansion and
            engraftment.
REFERENCE   6  (bases 1 to 2020)
  AUTHORS   Palty,R., Raveh,A., Kaminsky,I., Meller,R. and Reuveny,E.
  TITLE     SARAF inactivates the store operated calcium entry machinery to
            prevent excess calcium refilling
  JOURNAL   Cell 149 (2), 425-438 (2012)
   PUBMED   22464749
  REMARK    GeneRIF: Study shows that SARAF is an endoplasmic reticulum
            resident protein, which responds to cytosolic Ca2+ elevation after
            ER Ca2+ refilling by promoting a slow inactivation process of
            STIM2-dependent basal SOCE activity, as well as STIM1-mediated SOCE
            activity.
REFERENCE   7  (bases 1 to 2020)
  AUTHORS   Lodola,F., Laforenza,U., Bonetti,E., Lim,D., Dragoni,S.,
            Bottino,C., Ong,H.L., Guerra,G., Ganini,C., Massa,M., Manzoni,M.,
            Ambudkar,I.S., Genazzani,A.A., Rosti,V., Pedrazzoli,P., Tanzi,F.,
            Moccia,F. and Porta,C.
  TITLE     Store-operated Ca2+ entry is remodelled and controls in vitro
            angiogenesis in endothelial progenitor cells isolated from tumoral
            patients
  JOURNAL   PLoS One 7 (9), e42541 (2012)
   PUBMED   23049731
  REMARK    GeneRIF: Store-operated Ca2+ entry is remodelled and controls in
            vitro angiogenesis in endothelial progenitor cells isolated from
            tumoral patients.
REFERENCE   8  (bases 1 to 2020)
  AUTHORS   Zhang,Z. and Henzel,W.J.
  TITLE     Signal peptide prediction based on analysis of experimentally
            verified cleavage sites
  JOURNAL   Protein Sci 13 (10), 2819-2824 (2004)
   PUBMED   15340161
REFERENCE   9  (bases 1 to 2020)
  AUTHORS   Clark,H.F., Gurney,A.L., Abaya,E., Baker,K., Baldwin,D., Brush,J.,
            Chen,J., Chow,B., Chui,C., Crowley,C., Currell,B., Deuel,B.,
            Dowd,P., Eaton,D., Foster,J., Grimaldi,C., Gu,Q., Hass,P.E.,
            Heldens,S., Huang,A., Kim,H.S., Klimowski,L., Jin,Y., Johnson,S.,
            Lee,J., Lewis,L., Liao,D., Mark,M., Robbie,E., Sanchez,C.,
            Schoenfeld,J., Seshagiri,S., Simmons,L., Singh,J., Smith,V.,
            Stinson,J., Vagts,A., Vandlen,R., Watanabe,C., Wieand,D., Woods,K.,
            Xie,M.H., Yansura,D., Yi,S., Yu,G., Yuan,J., Zhang,M., Zhang,Z.,
            Goddard,A., Wood,W.I., Godowski,P. and Gray,A.
  TITLE     The secreted protein discovery initiative (SPDI), a large-scale
            effort to identify novel human secreted and transmembrane proteins:
            a bioinformatics assessment
  JOURNAL   Genome Res 13 (10), 2265-2270 (2003)
   PUBMED   12975309
  REMARK    Erratum:[Genome Res. 2003 Dec;13(12):2759]
REFERENCE   10 (bases 1 to 2020)
  AUTHORS   Chen,J., Sun,M., Lee,S., Zhou,G., Rowley,J.D. and Wang,S.M.
  TITLE     Identifying novel transcripts and novel genes in the human genome
            by using novel SAGE tags
  JOURNAL   Proc Natl Acad Sci U S A 99 (19), 12257-12262 (2002)
   PUBMED   12213963
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC044849.12.
            
            On May 17, 2019 this sequence version replaced NM_016127.5.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803612.17909.1,
                                           SRR1660809.27298.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA2142348
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000256255.11/ ENSP00000256255.6
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-218               AC044849.12        116689-116906       c
            219-397             AC044849.12        107719-107897       c
            398-815             AC044849.12        103484-103901       c
            816-957             AC044849.12        100619-100760       c
            958-1109            AC044849.12        99830-99981         c
            1110-2020           AC044849.12        96845-97755         c
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8p12"
     gene            1..2020
                     /gene="SARAF"
                     /gene_synonym="FOAP-7; HSPC035; TMEM66; XTP3"
                     /note="store-operated calcium entry associated regulatory
                     factor"
                     /db_xref="GeneID:51669"
                     /db_xref="HGNC:HGNC:28789"
                     /db_xref="MIM:614768"
     exon            1..218
                     /gene="SARAF"
                     /gene_synonym="FOAP-7; HSPC035; TMEM66; XTP3"
                     /inference="alignment:Splign:2.1.0"
     CDS             116..1135
                     /gene="SARAF"
                     /gene_synonym="FOAP-7; HSPC035; TMEM66; XTP3"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; HBV XAg-transactivated protein 3; HBV
                     X-transactivated gene 3 protein; SOCE-associated
                     regulatory factor; transmembrane protein 66; testicular
                     secretory protein Li 59"
                     /codon_start=1
                     /product="store-operated calcium entry-associated
                     regulatory factor isoform 1 precursor"
                     /protein_id="NP_057211.4"
                     /db_xref="CCDS:CCDS6074.1"
                     /db_xref="GeneID:51669"
                     /db_xref="HGNC:HGNC:28789"
                     /db_xref="MIM:614768"
                     /translation="MAAACGPGAAGYCLLLGLHLFLLTAGPALGWNDPDRMLLRDVKA
                     LTLHYDRYTTSRRLDPIPQLKCVGGTAGCDSYTPKVIQCQNKGWDGYDVQWECKTDLD
                     IAYKFGKTVVSCEGYESSEDQYVLRGSCGLEYNLDYTELGLQKLKESGKQHGFASFSD
                     YYYKWSSADSCNMSGLITIVVLLGIAFVVYKLFLSDGQYSPPPYSEYPPFSHRYQRFT
                     NSAGPPPPGFKSEFTGPQNTGHGATSGFGSAFTGQQGYENSGPGFWTGLGTGGILGYL
                     FGSNRAATPFSDSWYYPSYPPSYPGTWNRAYSPLHGGSGSYSVCSNSDTKTRTASGYG
                     GTRRR"
     exon            219..397
                     /gene="SARAF"
                     /gene_synonym="FOAP-7; HSPC035; TMEM66; XTP3"
                     /inference="alignment:Splign:2.1.0"
     exon            398..815
                     /gene="SARAF"
                     /gene_synonym="FOAP-7; HSPC035; TMEM66; XTP3"
                     /inference="alignment:Splign:2.1.0"
     exon            816..957
                     /gene="SARAF"
                     /gene_synonym="FOAP-7; HSPC035; TMEM66; XTP3"
                     /inference="alignment:Splign:2.1.0"
     exon            958..1109
                     /gene="SARAF"
                     /gene_synonym="FOAP-7; HSPC035; TMEM66; XTP3"
                     /inference="alignment:Splign:2.1.0"
     exon            1110..2020
                     /gene="SARAF"
                     /gene_synonym="FOAP-7; HSPC035; TMEM66; XTP3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1908..1913
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SARAF"
                     /gene_synonym="FOAP-7; HSPC035; TMEM66; XTP3"
                     /note="hexamer: AATAAA"
     regulatory      1918..1923
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SARAF"
                     /gene_synonym="FOAP-7; HSPC035; TMEM66; XTP3"
                     /note="hexamer: AACAAG"
     polyA_site      1926
                     /gene="SARAF"
                     /gene_synonym="FOAP-7; HSPC035; TMEM66; XTP3"
                     /note="major polyA site"
     regulatory      1960..1965
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SARAF"
                     /gene_synonym="FOAP-7; HSPC035; TMEM66; XTP3"
                     /note="hexamer: AATAAA"
     polyA_site      1961
                     /gene="SARAF"
                     /gene_synonym="FOAP-7; HSPC035; TMEM66; XTP3"
     polyA_site      1987
                     /gene="SARAF"
                     /gene_synonym="FOAP-7; HSPC035; TMEM66; XTP3"
ORIGIN      
        1 cgttccttcg ccgccgccag gggtagcggt gtagctgcgc agcgtcgcgc gcgctaccgc
       61 acccaggttc ggcccgtagg cgtctggcag cccggcgcca tcttcatcga gcgccatggc
      121 cgcagcctgc gggccgggag cggccgggta ctgcttgctc ctcggcttgc atttgtttct
      181 gctgaccgcg ggccctgccc tgggctggaa cgaccctgac agaatgttgc tgcgggatgt
      241 aaaagctctt accctccact atgaccgcta taccacctcc cgcaggctgg atcccatccc
      301 acagttgaaa tgtgttggag gcacagctgg ttgtgattct tataccccaa aagtcataca
      361 gtgtcagaac aaaggctggg atgggtatga tgtacagtgg gaatgtaaga cggacttaga
      421 tattgcatac aaatttggaa aaactgtggt gagctgtgaa ggctatgagt cctctgaaga
      481 ccagtatgta ctaagaggtt cttgtggctt ggagtataat ttagattata cagaacttgg
      541 cctgcagaaa ctgaaggagt ctggaaagca gcacggcttt gcctctttct ctgattatta
      601 ttataagtgg tcctcggcgg attcctgtaa catgagtgga ttgattacca ttgtggtact
      661 ccttgggatc gcgtttgtag tctataagct gttcctgagt gacgggcagt attctcctcc
      721 accgtactct gagtatcctc cattttccca ccgttaccag agattcacca actcagcagg
      781 acctcctccc ccaggcttta agtctgagtt cacaggacca cagaatactg gccatggtgc
      841 aacttctggt tttggcagtg cttttacagg acaacaagga tatgaaaatt caggaccagg
      901 gttctggaca ggcttgggaa ctggtggaat actaggatat ttgtttggca gcaatagagc
      961 ggcaacaccc ttctcagact cgtggtacta cccgtcctat cctccctcct accctggcac
     1021 gtggaatagg gcttactcac cccttcatgg aggctcgggc agctattcgg tatgttcaaa
     1081 ctcagacacg aaaaccagaa ctgcatcagg atatggtggt accaggagac gataaagtag
     1141 aaagttggag tcaaacactg gatgcagaaa ttttggattt ttcatcactt tctctttaga
     1201 aaaaaagtac tacctgttaa caattgggaa aaggggatat tcaaaagttc tgtggtgtta
     1261 tgtccagtgt agctttttgt attctattat ttgaggctaa aagttgatgt gtgacaaaat
     1321 acttatgtgt tgtatgtcag tgtaacatgc agatgtatat tgcagttttt gaaagtgatc
     1381 attactgtgg aatgctaaaa atacattaat ttctaaaacc tgtgatgccc taagaagcat
     1441 taagaatgaa ggtgttgtac taatagaaac taagtacaga aaatttcagt tttaggtggt
     1501 tgtagctgat gagttattac ctcatagaga ctataatatt ctatttggta ttatattatt
     1561 tgatgtttgc tgttcttcaa acatttaaat caagctttgg actaattatg ctaatttgtg
     1621 agttctgatc acttttgagc tctgaagctt tgaatcattc agtggtggag atggccttct
     1681 ggtaactgaa tattaccttc tgtaggaaaa ggtagaaaat aagcatctag aaggttgttg
     1741 tgaatgactc tgtgctggca aaaatgcttg aaacctctat atttctttcg ttcataagag
     1801 gtaaaggtca aatttttcaa caaaagtctt ttaataacaa aagcatgcag ttctctgtga
     1861 aatctcaaat attgttgtaa tagtctgttt caatcttaaa aagaatcaat aaaaacaaac
     1921 aaggggttta gtctcattct ttctattagc ctaggcgcaa ataaatgtta ctttttgttt
     1981 ttttaaaccc tttttcccaa aggggaaagt tcatgcagaa
//
