LOCUS       NM_015589               7157 bp    mRNA    linear   PRI 02-APR-2024
DEFINITION  Homo sapiens sterile alpha motif domain containing 4A (SAMD4A),
            transcript variant 1, mRNA.
ACCESSION   NM_015589
VERSION     NM_015589.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7157)
  AUTHORS   Li,D., Qi,T. and Chen,J.
  TITLE     SAMD4A serves as a negative prognostic marker for gastric cancer
            patients
  JOURNAL   Tissue Cell 84, 102167 (2023)
   PUBMED   37515967
  REMARK    GeneRIF: SAMD4A serves as a negative prognostic marker for gastric
            cancer patients.
REFERENCE   2  (bases 1 to 7157)
  AUTHORS   Kubikova,J., Ubartaite,G., Metz,J. and Jeske,M.
  TITLE     Structural basis for binding of Drosophila Smaug to the GPCR
            Smoothened and to the germline inducer Oskar
  JOURNAL   Proc Natl Acad Sci U S A 120 (32), e2304385120 (2023)
   PUBMED   37523566
  REMARK    GeneRIF: Structural basis for binding of Drosophila Smaug to the
            GPCR Smoothened and to the germline inducer Oskar.
REFERENCE   3  (bases 1 to 7157)
  AUTHORS   Segal,D., Maier,S., Mastromarco,G.J., Qian,W.W., Nabeel-Shah,S.,
            Lee,H., Moore,G., Lacoste,J., Larsen,B., Lin,Z.Y.,
            Selvabaskaran,A., Liu,K., Smibert,C., Zhang,Z., Greenblatt,J.,
            Peng,J., Lee,H.O., Gingras,A.C. and Taipale,M.
  TITLE     A central chaperone-like role for 14-3-3 proteins in human cells
  JOURNAL   Mol Cell 83 (6), 974-993 (2023)
   PUBMED   36931259
REFERENCE   4  (bases 1 to 7157)
  AUTHORS   Zhou,M., Wang,B., Li,H., Han,J., Li,A. and Lu,W.
  TITLE     RNA-binding protein SAMD4A inhibits breast tumor angiogenesis by
            modulating the balance of angiogenesis program
  JOURNAL   Cancer Sci 112 (9), 3835-3845 (2021)
   PUBMED   34219323
  REMARK    GeneRIF: RNA-binding protein SAMD4A inhibits breast tumor
            angiogenesis by modulating the balance of angiogenesis program.
REFERENCE   5  (bases 1 to 7157)
  AUTHORS   Wang,W., Lv,R., Zhang,J. and Liu,Y.
  TITLE     circSAMD4A participates in the apoptosis and autophagy of
            dopaminergic neurons via the miR-29c-3p-mediated AMPK/mTOR pathway
            in Parkinson's disease
  JOURNAL   Mol Med Rep 24 (1) (2021)
   PUBMED   34080649
  REMARK    GeneRIF: circSAMD4A participates in the apoptosis and autophagy of
            dopaminergic neurons via the miR29c3pmediated AMPK/mTOR pathway in
            Parkinson's disease.
REFERENCE   6  (bases 1 to 7157)
  AUTHORS   Castello,A., Fischer,B., Eichelbaum,K., Horos,R., Beckmann,B.M.,
            Strein,C., Davey,N.E., Humphreys,D.T., Preiss,T., Steinmetz,L.M.,
            Krijgsveld,J. and Hentze,M.W.
  TITLE     Insights into RNA biology from an atlas of mammalian mRNA-binding
            proteins
  JOURNAL   Cell 149 (6), 1393-1406 (2012)
   PUBMED   22658674
REFERENCE   7  (bases 1 to 7157)
  AUTHORS   Baez,M.V., Luchelli,L., Maschi,D., Habif,M., Pascual,M.,
            Thomas,M.G. and Boccaccio,G.L.
  TITLE     Smaug1 mRNA-silencing foci respond to NMDA and modulate synapse
            formation
  JOURNAL   J Cell Biol 195 (7), 1141-1157 (2011)
   PUBMED   22201125
  REMARK    GeneRIF: The Smaug1 is expressed during synaptogenesis, and Smaug1
            knockdown affected the number and size of synapses, and also
            provoked an impaired response to repetitive depolarizing stimuli,
            as indicated by a reduced induction of Arc/Arg3.1.
REFERENCE   8  (bases 1 to 7157)
  AUTHORS   Baez,M.V. and Boccaccio,G.L.
  TITLE     Mammalian Smaug is a translational repressor that forms cytoplasmic
            foci similar to stress granules
  JOURNAL   J Biol Chem 280 (52), 43131-43140 (2005)
   PUBMED   16221671
  REMARK    GeneRIF: Smaug 1 has a role in RNA granule formation and
            translation regulation in neurons
REFERENCE   9  (bases 1 to 7157)
  AUTHORS   Benzinger,A., Muster,N., Koch,H.B., Yates,J.R. 3rd and Hermeking,H.
  TITLE     Targeted proteomic analysis of 14-3-3 sigma, a p53 effector
            commonly silenced in cancer
  JOURNAL   Mol Cell Proteomics 4 (6), 785-795 (2005)
   PUBMED   15778465
REFERENCE   10 (bases 1 to 7157)
  AUTHORS   Jin,J., Smith,F.D., Stark,C., Wells,C.D., Fawcett,J.P.,
            Kulkarni,S., Metalnikov,P., O'Donnell,P., Taylor,P., Taylor,L.,
            Zougman,A., Woodgett,J.R., Langeberg,L.K., Scott,J.D. and Pawson,T.
  TITLE     Proteomic, functional, and domain-based analysis of in vivo 14-3-3
            binding proteins involved in cytoskeletal regulation and cellular
            organization
  JOURNAL   Curr Biol 14 (16), 1436-1450 (2004)
   PUBMED   15324660
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL359792.3, DA393986.1, BC121174.1, AK024652.1, AB028976.1,
            AK074716.1 and AL133444.4.
            
            On Jul 25, 2019 this sequence version replaced NM_015589.5.
            
            Summary: Sterile alpha motifs (SAMs) in proteins such as SAMD4A are
            part of an RNA-binding domain that functions as a
            posttranscriptional regulator by binding to an RNA sequence motif
            known as the Smaug recognition element, which was named after the
            Drosophila Smaug protein (Baez and Boccaccio, 2005 [PubMed
            16221671]).[supplied by OMIM, Mar 2008].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR18074969.968774.1,
                                           SRR14038194.2856541.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMEA2142680
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000554335.6/ ENSP00000452535.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-211               AL359792.3         33096-33306         c
            212-327             AL359792.3         32823-32938         c
            328-375             DA393986.1         3-50
            376-634             DA393986.1         52-310
            635-1044            BC121174.1         5-414
            1045-2799           AK024652.1         411-2165
            2800-5430           AB028976.1         1540-4170
            5431-6329           AK074716.1         940-1838
            6330-7156           AB028976.1         5070-5896
            7157-7157           AL133444.4         142136-142136
FEATURES             Location/Qualifiers
     source          1..7157
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q22.2"
     gene            1..7157
                     /gene="SAMD4A"
                     /gene_synonym="SAMD4; SMAUG; SMAUG1; SMG; SMGA"
                     /note="sterile alpha motif domain containing 4A"
                     /db_xref="GeneID:23034"
                     /db_xref="HGNC:HGNC:23023"
                     /db_xref="MIM:610747"
     exon            1..211
                     /gene="SAMD4A"
                     /gene_synonym="SAMD4; SMAUG; SMAUG1; SMG; SMGA"
                     /inference="alignment:Splign:2.1.0"
     exon            212..828
                     /gene="SAMD4A"
                     /gene_synonym="SAMD4; SMAUG; SMAUG1; SMG; SMGA"
                     /inference="alignment:Splign:2.1.0"
     CDS             633..2789
                     /gene="SAMD4A"
                     /gene_synonym="SAMD4; SMAUG; SMAUG1; SMG; SMGA"
                     /note="isoform 1 is encoded by transcript variant 1;
                     protein Smaug homolog 1; SAM domain-containing protein 4A"
                     /codon_start=1
                     /product="protein Smaug homolog 1 isoform 1"
                     /protein_id="NP_056404.4"
                     /db_xref="CCDS:CCDS32084.2"
                     /db_xref="GeneID:23034"
                     /db_xref="HGNC:HGNC:23023"
                     /db_xref="MIM:610747"
                     /translation="MMFRDQVGVLAGWFKGWNECEQTVALLSLLKRVSQTQARFLQLC
                     LEHSLADCAELHVLEREANSPGIINQWQQESKDKVISLLLTHLPLLKPGNLDAKVEYM
                     KLLPKILAHSIEHNQHIEESRQLLSYALIHPATSLEDRSALAMWLNHLEDRTSTSFGG
                     QNRGRSDSVDYGQTHYYHQRQNSDDKLNGWQNSRDSGICINASNWQDKSMGCENGHVP
                     LYSSSSVPTTINTIGTSTSTILSGQAHHSPLKRSVSLTPPMNVPNQPLGHGWMSHEDL
                     RARGPQCLPSDHAPLSPQSSVASSGSGGSEHLEDQTTARNTFQEEGSGMKDVPAWLKS
                     LRLHKYAALFSQMTYEEMMALTECQLEAQNVTKGARHKIVISIQKLKERQNLLKSLER
                     DIIEGGSLRIPLQELHQMILTPIKAYSSPSTTPEARRREPQAPRQPSLMGPESQSPDC
                     KDGAAATGATATPSAGASGGLQPHQLSSCDGELAVAPLPEGDLPGQFTRVMGKVCTQL
                     LVSRPDEENISSYLQLIDKCLIHEAFTETQKKRLLSWKQQVQKLFRSFPRKTLLDISG
                     YRQQRNRGFGQSNSLPTAGSVGGGMGRRNPRQYQIPSRNVPSARLGLLGTSGFVSSNQ
                     RNTTATPTIMKQGRQNLWFANPGGSNSMPSRTHSSVQRTRSLPVHTSPQNMLMFQQPE
                     FQLPVTEPDINNRLESLCLSMTEHALGDGVDRTSTI"
     exon            829..1347
                     /gene="SAMD4A"
                     /gene_synonym="SAMD4; SMAUG; SMAUG1; SMG; SMGA"
                     /inference="alignment:Splign:2.1.0"
     exon            1348..1611
                     /gene="SAMD4A"
                     /gene_synonym="SAMD4; SMAUG; SMAUG1; SMG; SMGA"
                     /inference="alignment:Splign:2.1.0"
     exon            1612..1721
                     /gene="SAMD4A"
                     /gene_synonym="SAMD4; SMAUG; SMAUG1; SMG; SMGA"
                     /inference="alignment:Splign:2.1.0"
     exon            1722..1808
                     /gene="SAMD4A"
                     /gene_synonym="SAMD4; SMAUG; SMAUG1; SMG; SMGA"
                     /inference="alignment:Splign:2.1.0"
     exon            1809..2142
                     /gene="SAMD4A"
                     /gene_synonym="SAMD4; SMAUG; SMAUG1; SMG; SMGA"
                     /inference="alignment:Splign:2.1.0"
     exon            2143..2228
                     /gene="SAMD4A"
                     /gene_synonym="SAMD4; SMAUG; SMAUG1; SMG; SMGA"
                     /inference="alignment:Splign:2.1.0"
     exon            2229..2347
                     /gene="SAMD4A"
                     /gene_synonym="SAMD4; SMAUG; SMAUG1; SMG; SMGA"
                     /inference="alignment:Splign:2.1.0"
     exon            2348..2549
                     /gene="SAMD4A"
                     /gene_synonym="SAMD4; SMAUG; SMAUG1; SMG; SMGA"
                     /inference="alignment:Splign:2.1.0"
     exon            2550..2676
                     /gene="SAMD4A"
                     /gene_synonym="SAMD4; SMAUG; SMAUG1; SMG; SMGA"
                     /inference="alignment:Splign:2.1.0"
     exon            2677..2760
                     /gene="SAMD4A"
                     /gene_synonym="SAMD4; SMAUG; SMAUG1; SMG; SMGA"
                     /inference="alignment:Splign:2.1.0"
     exon            2761..7157
                     /gene="SAMD4A"
                     /gene_synonym="SAMD4; SMAUG; SMAUG1; SMG; SMGA"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      2972
                     /gene="SAMD4A"
                     /gene_synonym="SAMD4; SMAUG; SMAUG1; SMG; SMGA"
                     /note="major polyA site"
     regulatory      7139..7144
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SAMD4A"
                     /gene_synonym="SAMD4; SMAUG; SMAUG1; SMG; SMGA"
                     /note="hexamer: AATACA"
     polyA_site      7157
                     /gene="SAMD4A"
                     /gene_synonym="SAMD4; SMAUG; SMAUG1; SMG; SMGA"
                     /note="major polyA site"
ORIGIN      
        1 gcagccgcat tgcaagtttt ctgcgcgggg aagatctgtt gctggtgctg gcgttcttaa
       61 gcacggcgcc cgaggccacc ccaggcgggc cgtgagggga gcaccagccg ttggcagcgg
      121 cagcaacagg aagccgcggg gtctcctccc gcacctggga agcaggcttc tcgctgttac
      181 ccggtgggga atagcccatc tcccacatcg gtggtgatcg ccgctcggga atgcgggcgt
      241 gaagggtccg agttgccgcc cagcggggag gagaccccag gacgccggaa atcaccatcc
      301 caaagctgca agaagggggg agaaagcatt cttcattcag ttgtgtgcct tgtgggggat
      361 ttttaaaaag tcgttttttt ttttaaagaa acatttccgt gctactgtct gtcatcgtct
      421 ctggggaaat cagccagaac caccggaacg taactgaaac cagacaagag aggcaggagc
      481 ccaggcagta cctgcagcgt ccgggcacca gagccacctt ggaacaggaa cgcgtctccg
      541 gccgcggggc tgcggctccg ccaaactttg gggcgggcgg ggcgggctgg ggcgcccagg
      601 gggctctgta gaccgagggc ggccccctaa ccatgatgtt tcgcgaccag gtcggggtgc
      661 tggcgggctg gtttaagggc tggaacgagt gcgagcagac tgttgcgctg ctgtcgctgc
      721 tcaagcgcgt gagccagacc caggcccgct tcctccagct ctgcctggag cactcgctgg
      781 ccgactgcgc cgagctgcac gtcctcgaac gcgaggccaa cagccccgga atcattaacc
      841 aatggcaaca ggaatccaag gataaagtga tttccctcct gttaactcat ctgcctttgc
      901 tgaagccagg aaacctcgac gcgaaagtag aatatatgaa actgctgccc aaaatcctgg
      961 ctcactctat tgaacacaac cagcacattg aggagagcag gcagctgctg tcctatgctt
     1021 tgatacatcc agccacttcg ttagaagacc gtagtgcttt agccatgtgg ctgaatcact
     1081 tggaggaccg cacgtcgacc agctttggtg gccagaaccg aggccgctca gactctgtgg
     1141 attatggaca gacacactac tatcaccaaa gacagaactc tgatgacaag ctcaatgggt
     1201 ggcagaactc tcgggattct gggatttgca tcaatgcctc caactggcag gacaaaagca
     1261 tggggtgtga gaatggccat gtgcccctct actcctcctc atctgtcccc accacaatca
     1321 atacgattgg aaccagcaca agtacaattc tctcaggcca ggcacaccac agccctttga
     1381 aacgatctgt gtcccttacc ccacccatga atgtgccaaa ccagcctcta ggacatggat
     1441 ggatgtctca tgaggactta cgagctagag gaccccagtg cctcccatcc gatcatgccc
     1501 ccctgtctcc acaaagcagt gtagcctctt caggaagtgg tgggagtgaa cacttagaag
     1561 atcagaccac tgctcgtaac acattccaag aagaaggtag tgggatgaaa gatgttccag
     1621 cctggctgaa aagcctccgc ctgcacaaat atgccgcgct tttctcccag atgacctatg
     1681 aggagatgat ggccctcacc gagtgccagc tggaggcgca gaatgttacc aaaggtgcaa
     1741 gacacaaaat tgtcatcagt attcagaagc tcaaagaaag acaaaatctc ctgaagtctt
     1801 tggaaaggga catcatcgag gggggcagcc tgcgcatccc gctccaggaa ctgcaccaga
     1861 tgatcctgac tccgatcaag gcctacagct ccccgagcac cacccccgag gctcgccgcc
     1921 gggagcccca ggccccgcgt cagccctcac tgatgggccc cgagagccag agccccgact
     1981 gcaaagatgg ggccgcagcc actggcgcca cggccacccc ctcggccggg gccagcgggg
     2041 ggctccagcc gcaccagctg agcagctgcg atggggagct ggccgtcgcc cccctgccag
     2101 agggggacct ccccgggcag ttcacacgcg tcatggggaa agtgtgcaca cagctcttgg
     2161 tctccagacc tgatgaggaa aatataagtt cctatttaca gctcatagac aagtgtctaa
     2221 ttcatgaggc atttacagag acacagaaaa aaagattgtt gtcatggaaa cagcaggtgc
     2281 agaagctctt tcggtctttc cctcggaaaa cccttctaga catatcagga tatcgacagc
     2341 aaagaaatcg aggctttggg caatccaact ccctcccgac ggctggctct gtgggcggtg
     2401 gcatgggcag acggaacccg cgccagtacc agatcccctc tcggaacgtc ccttccgccc
     2461 gcctgggcct cttgggcacc agtggattcg tcagctccaa ccagcgcaac accacagcta
     2521 cccccaccat catgaaacaa ggaagacaga acctgtggtt tgccaacccc gggggcagca
     2581 atagcatgcc aagccgcacc cacagctcag tccagaggac ccgctcgctg cccgtgcaca
     2641 cttccccaca gaacatgctg atgttccagc agccagaatt ccagcttccc gtgaccgaac
     2701 ctgacatcaa caacaggctg gagtcgttgt gcctcagtat gaccgaacac gccctgggag
     2761 acggggttga ccggacctcc accatctaga agctgaagac gagagtgacc gcgctggccg
     2821 tgaaatcgac tgctgcgggt ccagtgtccg ccatcttcag ggttgcacag aatcctccaa
     2881 gatactttgc agcctttttt ccccctggtc cctctcccgt tttgattttg tgagagcgta
     2941 ggtcatcctc gtaaacatat cagtagacct ggggttggtt attttgtcat ttgtttctgt
     3001 catgggatgg tttggtgtgt ggggtgggga ggggtctcta gggaattatg agactgggag
     3061 gggggtggag ggaatgcagg tagctctctg gatggaacgg ggacagggga aagagtactg
     3121 ccatgaaaga gataggagac acataagagg acagcagaag ccctggccct ggggaggctt
     3181 ctcggaaggc ctggcttcac aggcaggcca cagaaggata tcgcgggcac gtgcacccaa
     3241 agcaagatag tggcttccct tttatatcca atctaatcct gattggatgt ccctgaggcc
     3301 cctgctggaa acagccatag gagagggccc atggcagtag gggaaagaag gaagaaattc
     3361 cctgcaacaa aacttcagct aaactttgat ttgtgtattg tttacataat aattttaaag
     3421 ggtacataat gtgtaaagag tttggataga acctctcttc atactatggt tttcgtaaag
     3481 gatctgttgt tgttacggat tcattttttc cctctatttt tataagagca gcagagttgt
     3541 cttctcaaaa cggctgccaa gctctgcttc ttgggaagat ggatgcagtc atgtaggcct
     3601 gagctgtccg tctttcaccg ttaggtggga ggagcgtatg ggtggacttg aaggacatgg
     3661 acgtggctgg aatgagcaca gcattgggtg agcgcacaag gatgaggaca tcatgtgatc
     3721 agttatgggt ttgctcgcag ggcacccaga gattctcaaa gaatcctgca gcctctttct
     3781 gtgctggatt tgcttgtcac ggagaggcct ccctcccttt ccaccccacc catggggcat
     3841 tatcctgtca ctcccagcct tgctccacac acacacaggt gggtacaagt tccactggag
     3901 gagaaaaggc aaggatggac tttttcccct tgtgagaggt ttgataccca gaaaatgagc
     3961 tcaaaacctt tacattaggg ttgcttgtag gaactggagc ctggaggctg catctacttc
     4021 acctgtcact gctgagggag aggaggggag aaaggcccag caacagcgta cagaggggtc
     4081 agtcagcaag tccagagagc acatgcaggg agatgtttgg cccacaccgc acagccccgc
     4141 catctccagt gggcgatgaa agatgtagga aaggttgatt tcaagatgga aatgacagcg
     4201 ctatccgcac agtatgaatt agggatttgc tgtgttagtt gatttatcca tccacacaga
     4261 ggggaggaaa aagacactcg ttacttggtg ggagattgag aaactgtggt acctaccaca
     4321 aagtaatagc tctgtttatg aagggcaaga aaggctacat ttcagaattt gacacagtgg
     4381 agggtattag aggaaatcaa agaggagttg tgtggaaaat caggttgtgt aaatgaaggt
     4441 atgaatgctc agccagaggc aagatcaggg agatggtaca aggcctgttt gttacatgga
     4501 tgagtcgggt gcctggttgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgaagtcag
     4561 agttgaaatt cagaagatca gactatggaa atcactgccc tttttttttt aaaagctaat
     4621 gaactacata cagacctaaa aggccttaat gaaatccgaa caattttctt ttactttcaa
     4681 gatcaaaaac atgcacccaa cccagccttg gatgccacca ccaggttttt gaagcccttt
     4741 tgagtctaag aaggtgagaa caatgtaacc atagaaagcc tttcgtgagc agagaaggct
     4801 tgaatccaca atttcattcg ctgaattaaa aaaaaaaggt tcagacctct ccttgggtga
     4861 ctaagttcta aagatgcaaa tccatgtgca gaaagagatg gcatttttta gttgattatt
     4921 tttaaccaag tgccattaac attcatcccc cacatccctt ttctaaacaa gcttagtgtt
     4981 acttggggaa tgtgttggga tggacgatca catgtaaggc aggaagaata ggtcaaaggt
     5041 tgaaggccaa aggtaagacc agagggtttg cgaatgtggg tttgtaggat actgagaaag
     5101 tgaataaaga ggagaaaaaa ccatggtatt acacatcttg ctgagaaagg aaagcattcg
     5161 gatctgctgc aaaaacacat atatccataa agactcatgt tattcagaaa acagattgtg
     5221 aacacaatca cattcgcatg aatcctttaa aaggaagaag accttaaagt atctgcaaat
     5281 ctgaatttct atttattcct tcactgaata tagaaacaat ggttatctga ttattagaga
     5341 tattattttg gatatgttac ttattaactt gctatggctg gtaaccatga taaagtctgt
     5401 tattaataac aacataattc tttttttaaa gaagaaaagc ttatttttca ttgacagtgt
     5461 atagatttat ctacttagtt gtgttttgct attagtgttt taattttttt tttaagttga
     5521 gtgtttgata aattttaaga ccctgtcccc accttgtttt gagtcctgtg ttgactacag
     5581 gtatatagct caatttaaaa atcctaaagc aaaagaattt tatttataaa agaatcaaac
     5641 agttgcatgc atgaggctgt gaagtcagat atttagtaat aaaagcagca gtgccttttt
     5701 ttgtatttac ccattgaccc ccaccaaatg caactgtttt atattaagaa aatagtaaca
     5761 attttaaaat ctcagagtaa aatctatttc actacatgct tttcccccct tgttctgatt
     5821 taagcagtgt gtacttggca tctctacatt gtcctaggga cagtggtgtt ctacaatatt
     5881 atcatgtatg atgttttatt ggtgcttttt attcatagtg gcttcttacc agaaacagta
     5941 ggaagaaaca catgaactgt gtacaagaca tgaaacattg ctgctgatat gttgtttttt
     6001 cacatgcttt tgagttttca ctttttaaac gagagccagc aagcaaaata gatgtggctg
     6061 ggtctgcctg tccgggcggc tctttgcacc gagctctcaa atcctgtgta ttgagggttc
     6121 ctttttggta ctcaggattg gagctacagc tgggcccccc tctctcccat tcgtttgaag
     6181 agacactgag ggaaacaagg gtttcttttg aggtgtcctt ggctgccttt tacgggatgg
     6241 gagccttctc cggatctttt gttcttctgc acctcttgta gctactgccg gtgcaaggtt
     6301 gtagatgtta ttccccagga gcctgggctt gggggctgag ctgggctgaa tgcaaaagca
     6361 tgcaaccaga aggcgggcaa ggggaggaaa agcaggcctg gcctcattgg tcccctggag
     6421 atgtctgtag cagtcagctc cagcttgggc ctggggaagc agcctgacca aggcgctcag
     6481 gtgtgcctgt tacaagaaga acctgcagaa ggataatttg cacatggagc tgtgataaca
     6541 ctaatgttga tttttttttt ttttacaagt catcagagat gtttgcaaag tgagttttat
     6601 ttttttgtaa ttcctttatc tttacttaaa ggtgaatgtg tattcctctg ggaggaatag
     6661 gaagaaaaca ggaatgttaa taatgtcgaa cagaaaactt cctcccttat taatatataa
     6721 tcctcatgta tttatgccta atgtaagctg acttttaaaa agctttcttt tgttgcatgc
     6781 cctgtgcagg catctgtatt gtacatgcat gcctttcgtc ctgttttcct gtataaagtt
     6841 agtgaacaaa gaaatatttt tgcctagttc atgttgccaa gcaatgcata ttttttaaat
     6901 ttgtcatata tggaaagagc atgtttgtta catgtaaaag ctttactgat atacagatat
     6961 actaatgttt gaagatgctg ttctttgcaa gtgtacagtt ttcaaatgtt gttaccagtg
     7021 aaacaccctt gtggtttaaa cttgctacaa tgtatttatt attcatttcc tcccatgtaa
     7081 ctaagaatca tggctatatt tcatatcaac gttatattga aagtgaaggg aaatgattaa
     7141 tacaaggttt tgtaaca
//
