LOCUS       NM_171999               6007 bp    mRNA    linear   PRI 11-JUN-2024
DEFINITION  Homo sapiens spalt like transcription factor 3 (SALL3), mRNA.
ACCESSION   NM_171999
VERSION     NM_171999.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6007)
  AUTHORS   Lei,Y., Al Delbany,D., Krivec,N., Regin,M., Couvreu de
            Deckersberg,E., Janssens,C., Ghosh,M., Sermon,K. and Spits,C.
  TITLE     SALL3 mediates the loss of neuroectodermal differentiation
            potential in human embryonic stem cells with chromosome 18q loss
  JOURNAL   Stem Cell Reports 19 (4), 562-578 (2024)
   PUBMED   38552632
  REMARK    GeneRIF: SALL3 mediates the loss of neuroectodermal differentiation
            potential in human embryonic stem cells with chromosome 18q loss.
REFERENCE   2  (bases 1 to 6007)
  AUTHORS   Zhao,J., Wei,X., Yang,T., Zhao,M.Y., Pei,M.L. and Yang,X.F.
  TITLE     [Correlation between Methylation of SALL3 and Cervical Cancer]
  JOURNAL   Zhongguo Yi Xue Ke Xue Yuan Xue Bao 41 (5), 609-614 (2019)
   PUBMED   31699190
  REMARK    GeneRIF: Hypermethylation of SALL3 in promoter regions inhibits the
            expression of SALL3 in cervical cancer. Infection with high-risk
            HPV serotypes might increase the methylation of SALL3 promoter
            region, silence its expression,and thus promote the development of
            cervical cancer.
REFERENCE   3  (bases 1 to 6007)
  AUTHORS   Kuroda,T., Yasuda,S., Tachi,S., Matsuyama,S., Kusakawa,S., Tano,K.,
            Miura,T., Matsuyama,A. and Sato,Y.
  TITLE     SALL3 expression balance underlies lineage biases in human induced
            pluripotent stem cell differentiation
  JOURNAL   Nat Commun 10 (1), 2175 (2019)
   PUBMED   31092818
  REMARK    GeneRIF: SALL3 expression in human induced pluripotent stem cells
            (hiPSCs) correlates positively with ectoderm differentiation
            capacity and negatively with mesoderm/endoderm differentiation
            capacity. Without affecting self-renewal of hiPSCs, SALL3 knockdown
            inhibits ectoderm differentiation and conversely enhances
            mesodermal/endodermal differentiation.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6007)
  AUTHORS   Misawa,K., Mochizuki,D., Imai,A., Misawa,Y., Endo,S., Mima,M.,
            Kawasaki,H., Carey,T.E. and Kanazawa,T.
  TITLE     Epigenetic silencing of SALL3 is an independent predictor of poor
            survival in head and neck cancer
  JOURNAL   Clin Epigenetics 9, 64 (2017)
   PUBMED   28616099
  REMARK    GeneRIF: Results found that SALL3 promoter methylation was
            associated with its transcriptional inhibition. Also,
            hypermethylation of CpG islands in the SALL3 promoter was
            independently associated with aggressive behavior of head and neck
            cancer (HNSCC) tumors, suggesting that SALL3 acts as a tumor
            suppressor gene and can serve as a prognostic biomarker in HNSCC.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6007)
  AUTHORS   Eeles,R.A., Olama,A.A., Benlloch,S., Saunders,E.J.,
            Leongamornlert,D.A., Tymrakiewicz,M., Ghoussaini,M., Luccarini,C.,
            Dennis,J., Jugurnauth-Little,S., Dadaev,T., Neal,D.E., Hamdy,F.C.,
            Donovan,J.L., Muir,K., Giles,G.G., Severi,G., Wiklund,F.,
            Gronberg,H., Haiman,C.A., Schumacher,F., Henderson,B.E., Le
            Marchand,L., Lindstrom,S., Kraft,P., Hunter,D.J., Gapstur,S.,
            Chanock,S.J., Berndt,S.I., Albanes,D., Andriole,G., Schleutker,J.,
            Weischer,M., Canzian,F., Riboli,E., Key,T.J., Travis,R.C.,
            Campa,D., Ingles,S.A., John,E.M., Hayes,R.B., Pharoah,P.D.,
            Pashayan,N., Khaw,K.T., Stanford,J.L., Ostrander,E.A.,
            Signorello,L.B., Thibodeau,S.N., Schaid,D., Maier,C., Vogel,W.,
            Kibel,A.S., Cybulski,C., Lubinski,J., Cannon-Albright,L.,
            Brenner,H., Park,J.Y., Kaneva,R., Batra,J., Spurdle,A.B.,
            Clements,J.A., Teixeira,M.R., Dicks,E., Lee,A., Dunning,A.M.,
            Baynes,C., Conroy,D., Maranian,M.J., Ahmed,S., Govindasami,K.,
            Guy,M., Wilkinson,R.A., Sawyer,E.J., Morgan,A., Dearnaley,D.P.,
            Horwich,A., Huddart,R.A., Khoo,V.S., Parker,C.C., Van As,N.J.,
            Woodhouse,C.J., Thompson,A., Dudderidge,T., Ogden,C., Cooper,C.S.,
            Lophatananon,A., Cox,A., Southey,M.C., Hopper,J.L., English,D.R.,
            Aly,M., Adolfsson,J., Xu,J., Zheng,S.L., Yeager,M., Kaaks,R.,
            Diver,W.R., Gaudet,M.M., Stern,M.C., Corral,R., Joshi,A.D.,
            Shahabi,A., Wahlfors,T., Tammela,T.L., Auvinen,A., Virtamo,J.,
            Klarskov,P., Nordestgaard,B.G., Roder,M.A., Nielsen,S.F.,
            Bojesen,S.E., Siddiq,A., Fitzgerald,L.M., Kolb,S., Kwon,E.M.,
            Karyadi,D.M., Blot,W.J., Zheng,W., Cai,Q., McDonnell,S.K.,
            Rinckleb,A.E., Drake,B., Colditz,G., Wokolorczyk,D.,
            Stephenson,R.A., Teerlink,C., Muller,H., Rothenbacher,D.,
            Sellers,T.A., Lin,H.Y., Slavov,C., Mitev,V., Lose,F.,
            Srinivasan,S., Maia,S., Paulo,P., Lange,E., Cooney,K.A.,
            Antoniou,A.C., Vincent,D., Bacot,F., Tessier,D.C., Kote-Jarai,Z.
            and Easton,D.F.
  CONSRTM   COGS-Cancer Research UK GWAS-ELLIPSE (part of GAME-ON) Initiative;
            Australian Prostate Cancer Bioresource; UK Genetic Prostate Cancer
            Study Collaborators/British Association of Urological Surgeons'
            Section of Oncology; UK ProtecT (Prostate testing for cancer and
            Treatment) Study Collaborators; PRACTICAL (Prostate Cancer
            Association Group to Investigate Cancer-Associated Alterations in
            the Genome) Consortium
  TITLE     Identification of 23 new prostate cancer susceptibility loci using
            the iCOGS custom genotyping array
  JOURNAL   Nat Genet 45 (4), 385-391 (2013)
   PUBMED   23535732
REFERENCE   6  (bases 1 to 6007)
  AUTHORS   Shikauchi,Y., Saiura,A., Kubo,T., Niwa,Y., Yamamoto,J., Murase,Y.
            and Yoshikawa,H.
  TITLE     SALL3 interacts with DNMT3A and shows the ability to inhibit CpG
            island methylation in hepatocellular carcinoma
  JOURNAL   Mol Cell Biol 29 (7), 1944-1958 (2009)
   PUBMED   19139273
  REMARK    GeneRIF: This functional characterization of SALL3 sheds light on
            regulatory mechanisms for DNMT3A and provides new strategies to
            inhibit aberrant methylation in cancer.
REFERENCE   7  (bases 1 to 6007)
  AUTHORS   de Celis,J.F. and Barrio,R.
  TITLE     Regulation and function of Spalt proteins during animal development
  JOURNAL   Int J Dev Biol 53 (8-10), 1385-1398 (2009)
   PUBMED   19247946
  REMARK    GeneRIF: Studies indicate that vertebrate sal orthologues
            (spalt-like/sall) have important developmental roles during neural
            development and organogenesis and gentic diseases.
            Review article
REFERENCE   8  (bases 1 to 6007)
  AUTHORS   Julia,A., Ballina,J., Canete,J.D., Balsa,A., Tornero-Molina,J.,
            Naranjo,A., Alperi-Lopez,M., Erra,A., Pascual-Salcedo,D.,
            Barcelo,P., Camps,J. and Marsal,S.
  TITLE     Genome-wide association study of rheumatoid arthritis in the
            Spanish population: KLF12 as a risk locus for rheumatoid arthritis
            susceptibility
  JOURNAL   Arthritis Rheum 58 (8), 2275-2286 (2008)
   PUBMED   18668548
  REMARK    Erratum:[Arthritis Rheum. 2008 Oct;58(10):3276]
REFERENCE   9  (bases 1 to 6007)
  AUTHORS   Sweetman,D. and Munsterberg,A.
  TITLE     The vertebrate spalt genes in development and disease
  JOURNAL   Dev Biol 293 (2), 285-293 (2006)
   PUBMED   16545361
  REMARK    Review article
REFERENCE   10 (bases 1 to 6007)
  AUTHORS   Kohlhase,J., Hausmann,S., Stojmenovic,G., Dixkens,C., Bink,K.,
            Schulz-Schaeffer,W., Altmann,M. and Engel,W.
  TITLE     SALL3, a new member of the human spalt-like gene family, maps to
            18q23
  JOURNAL   Genomics 62 (2), 216-222 (1999)
   PUBMED   10610715
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC099689.4.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Sep 18, 2020 this sequence version replaced NM_171999.3.
            
            Summary: This gene encodes a sal-like C2H2-type zinc-finger
            protein, and belongs to a family of evolutionarily conserved genes
            found in species as diverse as Drosophila, C. elegans, and
            vertebrates. Mutations in some of these genes are associated with
            congenital disorders in human, suggesting their importance in
            embryonic development. This protein binds to DNA methyltransferase
            3 alpha (DNMT3A), and reduces DNMT3A-mediated CpG island
            methylation. It is suggested that silencing of this gene, resulting
            in acceleration of DNA methylation, may have a role in oncogenesis.
            [provided by RefSeq, Oct 2011].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: GQ900962.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2151119, SAMEA2153427
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000537592.7/ ENSP00000441823.2
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-539               AC099689.4         92340-92878
            540-3928            AC099689.4         104596-107984
            3929-6007           AC099689.4         109413-111491
FEATURES             Location/Qualifiers
     source          1..6007
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="18"
                     /map="18q23"
     gene            1..6007
                     /gene="SALL3"
                     /gene_synonym="ZNF796"
                     /note="spalt like transcription factor 3"
                     /db_xref="GeneID:27164"
                     /db_xref="HGNC:HGNC:10527"
                     /db_xref="MIM:605079"
     exon            1..539
                     /gene="SALL3"
                     /gene_synonym="ZNF796"
                     /inference="alignment:Splign:2.1.0"
     CDS             458..4360
                     /gene="SALL3"
                     /gene_synonym="ZNF796"
                     /note="C2H2 zinc finger protein SALL3; spalt-like zinc
                     finger protein; hSALL3; zinc finger protein 796;
                     epididymis secretory sperm binding protein"
                     /codon_start=1
                     /product="sal-like protein 3"
                     /protein_id="NP_741996.2"
                     /db_xref="CCDS:CCDS12013.1"
                     /db_xref="GeneID:27164"
                     /db_xref="HGNC:HGNC:10527"
                     /db_xref="MIM:605079"
                     /translation="MSRRKQAKPQHLKSDEELLPPDGAPEHAAPGEGAEDADSGPESR
                     SGGEETSVCEKCCAEFFKWADFLEHQRSCTKLPPVLIVHEDAPAPPPEDFPEPSPASS
                     PSERAESEAAEEAGAEGAEGEARPVEKEAEPMDAEPAGDTRAPRPPPAAPAPPTPAYG
                     APSTNVTLEALLSTKVAVAQFSQGARAAGGSGAGGGVAAAAVPLILEQLMALQQQQIH
                     QLQLIEQIRSQVALMQRPPPRPSLSPAAAPSAPGPAPSQLPGLAALPLSAGAPAAAIA
                     GSGPAAPAAFEGAQPLSRPESGASTPGGPAEPSAPAAPSAAPAPAAPAPAPAPQSAAS
                     SQPQSASTPPALAPGSLLGAAPGLPSPLLPQTSASGVIFPNPLVSIAATANALDPLSA
                     LMKHRKGKPPNVSVFEPKASAEDPFFKHKCRFCAKVFGSDSALQIHLRSHTGERPFKC
                     NICGNRFSTKGNLKVHFQRHKEKYPHIQMNPYPVPEYLDNVPTCSGIPYGMSLPPEKP
                     VTTWLDSKPVLPTVPTSVGLQLPPTVPGAHGYADSPSATPASRSPQRPSPASSECASL
                     SPGLNHVESGVSATAESPQSLLGGPPLTKAEPVSLPCTNARAGDAPVGAQASAAPTSV
                     DGAPTSLGSPGLPAVSEQFKAQFPFGGLLDSMQTSETSKLQQLVENIDKKMTDPNQCV
                     ICHRVLSCQSALKMHYRTHTGERPFKCKICGRAFTTKGNLKTHFGVHRAKPPLRVQHS
                     CPICQKKFTNAVVLQQHIRMHMGGQIPNTPLPEGFQDAMDSELAYDDKNAETLSSYDD
                     DMDENSMEDDAELKDAATDPAKPLLSYAGSCPPSPPSVISSIAALENQMKMIDSVMSC
                     QQLTGLKSVENGSGESDRLSNDSSSAVGDLESRSAGSPALSESSSSQALSPAPSNGES
                     FRSKSPGLGAPEEPQEIPLKTERPDSPAAAPGSGGAPGRAGIKEEAPFSLLFLSRERG
                     KCPSTVCGVCGKPFACKSALEIHYRSHTKERPFVCALCRRGCSTMGNLKQHLLTHRLK
                     ELPSQLFDPNFALGPSQSTPSLISSAAPTMIKMEVNGHGKAMALGEGPPLPAGVQVPA
                     GPQTVMGPGLAPMLAPPPRRTPKQHNCQSCGKTFSSASALQIHERTHTGEKPFGCTIC
                     GRAFTTKGNLKVHMGTHMWNNAPARRGRRLSVENPMALLGGDALKFSEMFQKDLAARA
                     MNVDPSFWNQYAAAITNGLAMKNNEISVIQNGGIPQLPVSLGGSALPPLGSMASGMDK
                     ARTGSSPPIVSLDKASSETAASRPFTRFIEDNKEIGIN"
     exon            540..3928
                     /gene="SALL3"
                     /gene_synonym="ZNF796"
                     /inference="alignment:Splign:2.1.0"
     exon            3929..6007
                     /gene="SALL3"
                     /gene_synonym="ZNF796"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5227..5232
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SALL3"
                     /gene_synonym="ZNF796"
                     /note="hexamer: AATAAA"
     polyA_site      5250
                     /gene="SALL3"
                     /gene_synonym="ZNF796"
                     /note="major polyA site"
ORIGIN      
        1 ggagacggcg ccgcgcggac gccgccaaag tttgctgcct gcgccctgcg gagggacggc
       61 caccgcggcc cgcgccgcac ccgggccccg ccacagccgc acccggggcg gccgaggagc
      121 gcggcgccgg agcccgcgat gtgaggcggc gccgggcagc gcgcgccccg gtcccgaggc
      181 gccgcggccc cctcctcgtc ggcgcggccg ctaattgcga gcgcggcctc atttgcatag
      241 gccgccggag tccgctggag cccggccaat cggcgcggcc ctccgctaat ggccatgcat
      301 tattcaccag cctaattgct cagccccatg cgcggcccgc gcagccgccg ccgccccgcg
      361 ccccgcgccg cgcgcccgcc aggccgcccc gcgccgtccc cgccggccgc cccgctgatg
      421 ccgctgcccc gcgcggggcc cgagcgccgc tagcagcatg tctcggcgca agcaggccaa
      481 gccccagcac ctcaagtcgg acgaggagct gctgccgcct gacggggctc ccgagcacgc
      541 cgccccgggg gaaggtgcgg aggacgcaga cagcgggccc gagagccgca gcgggggcga
      601 ggagaccagc gtgtgcgaga aatgctgcgc cgagttcttc aagtgggcgg acttcctgga
      661 gcaccagcgg agctgcacca agctcccgcc cgtgctgatc gtgcacgagg acgcgcccgc
      721 gccgcccccc gaggacttcc ccgagccttc gcccgccagc tcccccagcg agcgcgccga
      781 aagcgaggcg gccgaggagg cgggtgcgga gggcgcggag ggcgaggcca ggccggtgga
      841 gaaggaggcc gagcccatgg acgcggaacc cgcgggggac acgcgcgcgc cccggccccc
      901 gcctgcggcc cctgcacccc caacgcccgc ctacggcgcg cccagcacca acgtgaccct
      961 ggaggcgctg ctgagcacca aggtggcggt ggcgcagttc tcgcagggcg cgcgcgcggc
     1021 aggcggctcg ggagcaggtg gaggcgtggc agctgcagcc gtgcccctga tcctggaaca
     1081 gctcatggcc ctgcagcagc agcagatcca ccagctgcag ctcatcgagc agatccgcag
     1141 ccaggtggcc ctcatgcagc gcccgccgcc gcggccctca ctcagccccg cggccgcccc
     1201 gagcgcaccg ggcccggccc ccagccagct gcccgggctg gccgcgctcc cgctgtcggc
     1261 cggggcccct gccgccgcca tcgcgggctc gggccccgcc gccccggccg ccttcgaggg
     1321 cgcgcagccg ctgtcccggc ccgagtctgg cgccagcacc cccggcggcc ctgcggagcc
     1381 cagcgcgccc gccgccccca gcgccgcccc tgcccccgct gcccccgccc cggcgccagc
     1441 gccgcagagc gcagcctcgt cgcagccgca gagcgcatcc acgccgcctg ccctggcccc
     1501 ggggtccctg ctgggtgcgg cgcccggcct gccaagtccg cttctacctc agacttccgc
     1561 cagcggcgtc atcttcccca acccgctggt cagcatcgcg gccacggcca acgctctgga
     1621 cccgctgtcc gcgctcatga agcaccgcaa gggcaagccg cccaatgtgt cggtgttcga
     1681 gcccaaagcc agcgccgagg acccgttctt caagcacaaa tgccgcttct gcgccaaggt
     1741 cttcggcagc gacagcgcgc tccagatcca cctgcgctcg cacacaggcg agcggccctt
     1801 caagtgcaac atctgcggga accgcttctc caccaaaggc aacctgaagg tgcacttcca
     1861 gaggcacaag gagaagtacc cccacatcca gatgaaccct tacccggtcc ccgagtacct
     1921 ggacaacgtg cccacctgct cgggcatccc ctacggcatg tcgctgcccc ccgagaagcc
     1981 cgtgaccacc tggctggaca gcaagcccgt gctgcccacc gtgcccacgt ccgtggggct
     2041 gcaactgccg cccactgtcc ctggcgcgca cggctacgcc gactctccca gcgccacccc
     2101 agccagccgc tccccgcaga ggccctcgcc cgcctccagc gagtgcgcct ccttgtcccc
     2161 aggcctcaac cacgtggagt ccggcgtgtc ggccaccgcc gagtccccac agtcgctcct
     2221 cggcgggccg cccctcacta aagccgagcc cgtcagcctg ccctgcacca acgccagggc
     2281 cggggacgct cccgtgggcg cgcaggctag cgctgcaccc acatcggtgg acggcgcacc
     2341 cacgagcctc ggcagccccg ggctgcccgc cgtctccgag cagttcaagg cccagtttcc
     2401 gttcgggggg ctgctagact cgatgcaaac gtcggaaacc tcgaagctgc agcagctggt
     2461 ggagaacatc gacaagaaga tgacggaccc gaaccagtgc gtcatctgcc accgggtgct
     2521 gagctgccag agcgcgctga agatgcacta ccggacgcac acgggggagc ggccgttcaa
     2581 gtgcaagatc tgcggccgcg ccttcaccac caagggcaac ctcaagacgc acttcggcgt
     2641 gcaccgtgca aagccgcccc tgcgcgtgca gcactcctgc cccatctgcc agaagaagtt
     2701 caccaacgcc gtggtcctgc agcagcacat ccgcatgcac atgggcggcc agatccccaa
     2761 cacgccgctg ccggagggct tccaggatgc catggactcc gagctggcct acgacgacaa
     2821 gaacgcggag accctgagca gctacgatga cgacatggac gagaactcca tggaggacga
     2881 cgctgagctg aaggacgcgg ccaccgaccc ggccaagcca ctcctgtcct acgcggggtc
     2941 ctgcccgccc tccccgccct cggtcatctc cagcattgcc gccctggaga accagatgaa
     3001 gatgatcgac tcggtcatga gctgccagca gctgaccggc ctcaagtccg tggagaacgg
     3061 gtccggggag agtgaccgcc tgagcaacga ctcctcgtcg gccgtgggcg acctggagag
     3121 ccgcagcgcg ggcagccccg ccctgtccga gtcctcgtcc tcgcaggccc tgtcgccggc
     3181 ccccagcaat ggtgagagct tccgctccaa gtccccgggc ctgggcgccc cggaggagcc
     3241 ccaggaaatc ccgctcaaga ccgagaggcc ggacagccca gccgccgccc cgggcagcgg
     3301 aggcgcccct ggccgcgcgg gcatcaagga ggaggcgccc ttcagcctgc tgttcctgag
     3361 cagggagcgg ggtaagtgtc ccagcactgt gtgtggtgtc tgtggcaagc cttttgcttg
     3421 caagagcgcg ttggaaatcc actaccgcag ccatactaag gagcggccat tcgtctgcgc
     3481 gctctgcagg cgagggtgct ccactatggg taatttaaaa cagcacttac tgacacacag
     3541 attgaaagag ctgccttctc agttatttga ccccaacttt gctctaggtc ccagccaaag
     3601 cactcctagc ctgatctcca gcgccgcacc caccatgatc aaaatggaag tgaacggtca
     3661 cggcaaggcc atggcgctgg gcgagggtcc cccgctgccc gcgggcgtcc aggtccccgc
     3721 cgggcctcag acagtgatgg gcccgggcct ggcgcccatg ctggcccccc caccgcgccg
     3781 gacgcccaag cagcacaact gccagtcgtg cgggaagacc ttctcctcgg ccagcgccct
     3841 gcagatccat gagcgcacgc acaccggcga gaagccgttc ggctgcacca tctgcggccg
     3901 ggccttcacc actaagggca acctcaaggt gcacatgggg acacacatgt ggaataacgc
     3961 ccccgcgaga cgcggccgcc gcctgtctgt ggagaacccc atggctctcc tagggggtga
     4021 tgccctgaag ttctctgaaa tgttccagaa ggacctggca gctcgggcaa tgaacgtcga
     4081 ccccagtttt tggaaccagt atgctgcagc catcactaac gggctcgcca tgaagaacaa
     4141 cgagatctcc gtcatccaga acggcggcat cccccagctc cccgtgagtc ttgggggcag
     4201 cgccctcccc cctctgggca gcatggccag tgggatggac aaagcacgca ctggcagtag
     4261 cccacccatc gtcagcttgg acaaagcgag ctcagaaaca gcagccagcc gcccattcac
     4321 gcggtttatc gaggataaca aggagattgg tatcaactag ccagtgactc gctcatctgc
     4381 cctgcccagg cccacgtttt gaagttggag catcaggcct ccgacctttc ttgcctcggt
     4441 tctcattaca ctttcaccca tagcagaaaa cactttgtgc ggctgccgag aggtggtctt
     4501 gtaagcgctg catggcgctc ccttcaacag caagcctgac tgttctcgag aactctgcaa
     4561 tcttttaaat aagcttcctt caaaaaaaaa agtgcttgga aaaccgcctt aggaacagaa
     4621 agagctcaga ccatgtccac ttcctttctc ctgaaaccta ataatctctc cgagggagaa
     4681 aggggttctc tgcggtattc cagtgaaact catttgatgg tttcttttga attagttaga
     4741 cacttgaacg gtgtttttta gaactcttca tgttaaagac gtggtttagt actcccaatg
     4801 ctgtgtatca tgacactatc ttcgtctgta gtatttatga tgttaagata atgcgggtaa
     4861 cagacaatat aatagccccg accttaaacg aagcttttgt actgcagaat acatctggct
     4921 gtgtgatttt tttttttaag caagatttgt tttactataa ataagtggat tatttcaatg
     4981 caggcaaaat tgtgaagttc tgttgggaaa gatagcatgc ttttcgtgtg caagtacctg
     5041 tcagtaataa gccttttttt tttttttttt taatttaaat gtttgtagct gctatgtgga
     5101 cagttgtttt ctagtgtggt ctgtagccca ataactgggg aacgagttac agacaaacat
     5161 caccgtaaat gactcacaac attataaaca gttgtgagaa aatatttcac attatcaaag
     5221 ctgtacaata aaaaggtaat gtttgtataa tgtggttgca aactcttaat ttatactatt
     5281 gcacttttag tattttgtat tagggaggtt tgtctataat ttgaaaattt aaaaaaatgt
     5341 aaagtatttt ataaatagta cttcagttta aggaaaatgg gaaaactgtt acagttcctt
     5401 gttttgtctt atggtcaaac aatattagaa atctctgcca cttgaaaagc caccaagact
     5461 tgtcagcttt tcagtaaagt agggcctgcc gtttcttaaa ttaaaatgat ttatttaact
     5521 tttccacaaa gcccacttta aaactgtgac cgccccctaa cgaaaccttg gtgaattcat
     5581 cctaggacta tgacgtcagt acaagccagg aaggaactag gataaccttc tgcaggtctg
     5641 attttcatgt aagcgggtct gtctctctct ctcacccaca gacaagattg tggctgcctg
     5701 gtggcactgg tcacatgtac agtaaaccca agagaaatgg caagtatcag gcagtacaat
     5761 cagtcatgac tttgaaattt tccgagttcc tgatttattt attatcttct cagatgaaag
     5821 caaagcacag tgtcctctga tttttcagaa cacagcctga cgctgatggc tgagctgcat
     5881 ccagccagga gggccccgga ggctggggcg gccgaggcag cacaaacacg gcgtcgtgaa
     5941 ctacctcatt ttcgtgcgtt gcaagtgtgc tggcgtctga cactatgaac ttccaacaac
     6001 aaaattg
//
