LOCUS       NM_181885               1450 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens relaxin family peptide/INSL5 receptor 4 (RXFP4), mRNA.
ACCESSION   NM_181885
VERSION     NM_181885.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Chen,Y., Zhou,Q., Wang,J., Xu,Y., Wang,Y., Yan,J., Wang,Y., Zhu,Q.,
            Zhao,F., Li,C., Chen,C.W., Cai,X., Bathgate,R.A.D., Shen,C., Eric
            Xu,H., Yang,D., Liu,H. and Wang,M.W.
  TITLE     Ligand recognition mechanism of the human relaxin family peptide
            receptor 4 (RXFP4)
  JOURNAL   Nat Commun 14 (1), 492 (2023)
   PUBMED   36717591
  REMARK    GeneRIF: Ligand recognition mechanism of the human relaxin family
            peptide receptor 4 (RXFP4).
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1450)
  AUTHORS   Koo,A., Pustovit,R.V., Woodward,O.R.M., Lewis,J.E., Gribble,F.M.,
            Hossain,M.A., Reimann,F. and Furness,J.B.
  TITLE     Expression of the relaxin family peptide 4 receptor by
            enterochromaffin cells of the mouse large intestine
  JOURNAL   Cell Tissue Res 389 (1), 1-9 (2022)
   PUBMED   35596811
  REMARK    GeneRIF: Expression of the relaxin family peptide 4 receptor by
            enterochromaffin cells of the mouse large intestine.
REFERENCE   3  (bases 1 to 1450)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 1450)
  AUTHORS   Wang,J.H., Nie,W.H., Shao,X.X., Li,H.Z., Hu,M.J., Liu,Y.L., Xu,Z.G.
            and Guo,Z.Y.
  TITLE     Exploring electrostatic interactions of relaxin family peptide
            receptor 3 and 4 with ligands using a NanoBiT-based binding assay
  JOURNAL   Biochim Biophys Acta Biomembr 1861 (4), 776-786 (2019)
   PUBMED   30684458
  REMARK    GeneRIF: present work shed new light on the interaction mechanism
            of RXFP3 and RXFP4 with agonists and antagonists, and also provided
            a novel approach for interaction studies of some plasma membrane
            receptors with their ligands.
REFERENCE   5  (bases 1 to 1450)
  AUTHORS   Yeganeh,I.S., Taromchi,A.H., Fathabadi,F.F., Nejatbakhsh,R.,
            Novin,M.G. and Shokri,S.
  TITLE     Expression and localization of relaxin family peptide receptor 4 in
            human spermatozoa and impact of insulin-like peptide 5 on sperm
            functions
  JOURNAL   Reprod Biol 17 (4), 327-332 (2017)
   PUBMED   28986276
  REMARK    GeneRIF: The results confirmed the presence of RXFP4 in human
            spermatozoa, which localized in the neck and midpiece of sperm.
REFERENCE   6  (bases 1 to 1450)
  AUTHORS   Liu,C., Chen,J., Sutton,S., Roland,B., Kuei,C., Farmer,N.,
            Sillard,R. and Lovenberg,T.W.
  TITLE     Identification of relaxin-3/INSL7 as a ligand for GPCR142
  JOURNAL   J Biol Chem 278 (50), 50765-50770 (2003)
   PUBMED   14522967
  REMARK    GeneRIF: relaxin-3/INSL7 is a ligand for GPCR142 [GPCR142]
REFERENCE   7  (bases 1 to 1450)
  AUTHORS   Fredriksson,R., Hoglund,P.J., Gloriam,D.E., Lagerstrom,M.C. and
            Schioth,H.B.
  TITLE     Seven evolutionarily conserved human rhodopsin G protein-coupled
            receptors lacking close relatives
  JOURNAL   FEBS Lett 554 (3), 381-388 (2003)
   PUBMED   14623098
REFERENCE   8  (bases 1 to 1450)
  AUTHORS   Boels,K. and Schaller,H.C.
  TITLE     Identification and characterisation of GPR100 as a novel human
            G-protein-coupled bradykinin receptor
  JOURNAL   Br J Pharmacol 140 (5), 932-938 (2003)
   PUBMED   14530218
REFERENCE   9  (bases 1 to 1450)
  AUTHORS   Takeda,S., Kadowaki,S., Haga,T., Takaesu,H. and Mitaku,S.
  TITLE     Identification of G protein-coupled receptor genes from the human
            genome sequence
  JOURNAL   FEBS Lett 520 (1-3), 97-101 (2002)
   PUBMED   12044878
  REMARK    Erratum:[FEBS Lett 2002 Jul 17;523(1-3):257]
REFERENCE   10 (bases 1 to 1450)
  AUTHORS   Itoh,H., Toyama,R., Kozasa,T., Tsukamoto,T., Matsuoka,M. and
            Kaziro,Y.
  TITLE     Presence of three distinct molecular species of Gi protein alpha
            subunit. Structure of rat cDNAs and human genomic DNAs
  JOURNAL   J Biol Chem 263 (14), 6656-6664 (1988)
   PUBMED   2834384
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL355388.30.
            
            On Nov 27, 2019 this sequence version replaced NM_181885.2.
            
            Summary: GPR100 is a member of the rhodopsin family of G
            protein-coupled receptors (GPRs) (Fredriksson et al., 2003 [PubMed
            14623098]).[supplied by OMIM, Mar 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript is intronless :: BC101509.1, AY170824.1 [ECO:0000345]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000368318.5/ ENSP00000357301.4
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1450              AL355388.30        35318-36767
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q22"
     gene            1..1450
                     /gene="RXFP4"
                     /gene_synonym="GPCR142; GPR100; RLN3R2; RXFPR4"
                     /note="relaxin family peptide/INSL5 receptor 4"
                     /db_xref="GeneID:339403"
                     /db_xref="HGNC:HGNC:14666"
                     /db_xref="MIM:609043"
     exon            1..1450
                     /gene="RXFP4"
                     /gene_synonym="GPCR142; GPR100; RLN3R2; RXFPR4"
                     /inference="alignment:Splign:2.1.0"
     CDS             73..1197
                     /gene="RXFP4"
                     /gene_synonym="GPCR142; GPR100; RLN3R2; RXFPR4"
                     /note="G protein-coupled receptor 100; relaxin family
                     peptide receptor 4; RLN3 receptor 2; G-protein coupled
                     receptor GPCR142; insulin-like peptide INSL5 receptor;
                     relaxin/insulin like family peptide receptor 4"
                     /codon_start=1
                     /product="relaxin-3 receptor 2"
                     /protein_id="NP_871001.1"
                     /db_xref="CCDS:CCDS1124.1"
                     /db_xref="GeneID:339403"
                     /db_xref="HGNC:HGNC:14666"
                     /db_xref="MIM:609043"
                     /translation="MPTLNTSASPPTFFWANASGGSVLSADDAPMPVKFLALRLMVAL
                     AYGLVGAIGLLGNLAVLWVLSNCARRAPGPPSDTFVFNLALADLGLALTLPFWAAESA
                     LDFHWPFGGALCKMVLTATVLNVYASIFLITALSVARYWVVAMAAGPGTHLSLFWARI
                     ATLAVWAAAALVTVPTAVFGVEGEVCGVRLCLLRFPSRYWLGAYQLQRVVLAFMVPLG
                     VITTSYLLLLAFLQRRQRRRQDSRVVARSVRILVASFFLCWFPNHVVTLWGVLVKFDL
                     VPWNSTFYTIQTYVFPVTTCLAHSNSCLNPVLYCLLRREPRQALAGTFRDLRLRLWPQ
                     GGGWVQQVALKQVGRRWVASNPRESRPSTLLTNLDRGTPG"
     regulatory      1426..1431
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RXFP4"
                     /gene_synonym="GPCR142; GPR100; RLN3R2; RXFPR4"
                     /note="hexamer: AATAAA"
     polyA_site      1450
                     /gene="RXFP4"
                     /gene_synonym="GPCR142; GPR100; RLN3R2; RXFPR4"
                     /note="major polyA site"
ORIGIN      
        1 agtggcctct gccagtctgg cagacacttg cagacctctc ttctcagcac caccaatctc
       61 tgatgccctg cgatgcccac actcaatact tctgcctctc cacccacatt cttctgggcc
      121 aatgcctccg gaggcagtgt gctgagtgct gatgatgctc cgatgcctgt caaattccta
      181 gccctgaggc tcatggttgc cctggcctat gggcttgtgg gggccattgg cttgctggga
      241 aatttggcgg tgctgtgggt actgagtaac tgtgcccgga gagcccctgg cccaccttca
      301 gacaccttcg tcttcaacct ggctctggcg gacctgggac tggcactcac tctccccttt
      361 tgggcagccg agtcggcact ggactttcac tggcccttcg gaggtgccct ctgcaagatg
      421 gttctgacgg ccactgtcct caacgtctat gccagcatct tcctcatcac agcgctgagc
      481 gttgctcgct actgggtggt ggccatggct gcggggccag gcacccacct ctcactcttc
      541 tgggcccgaa tagccaccct ggcagtgtgg gcggcggctg ccctggtgac ggtgcccaca
      601 gctgtcttcg gggtggaggg tgaggtgtgt ggtgtgcgcc tttgcctgct gcgtttcccc
      661 agcaggtact ggctgggggc ctaccagctg cagagggtgg tgctggcttt catggtgccc
      721 ttgggcgtca tcaccaccag ctacctgctg ctgctggcct tcctgcagcg gcggcaacgg
      781 cggcggcagg acagcagggt cgtggcccgc tctgtccgca tcctggtggc ttccttcttc
      841 ctctgctggt ttcccaacca tgtggtcact ctctggggtg tcctggtgaa gtttgacctg
      901 gtgccctgga acagtacttt ctatactatc cagacgtatg tcttccctgt cactacttgc
      961 ttggcacaca gcaatagctg cctcaaccct gtgctgtact gtctcctgag gcgggagccc
     1021 cggcaggctc tggcaggcac cttcagggat ctgcggttga ggctgtggcc ccagggcgga
     1081 ggctgggtgc aacaggtggc cctaaagcag gtaggcaggc ggtgggtcgc aagcaacccc
     1141 cgggagagcc gcccttctac cctgctcacc aacctggaca gagggacacc cgggtgaagg
     1201 gcgcaagctg aacacactcc tctttctgag atccaccaag tgtaggatcc ttgagtcctg
     1261 gggagaagct gccctctctg ccaggctgca gtgccctcag ggaaaagtct gatctttgat
     1321 ccccaactct gggtgtggtg aatgggggag gcgggggctc agatcagagc tggatgtgac
     1381 aaagcttaag tctttatttg gagatgggaa agaagaggat ctgagaataa acctctggat
     1441 tatccacaaa
//
