LOCUS       NM_152682               2601 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens RWD domain containing 4 (RWDD4), transcript variant 1,
            mRNA.
ACCESSION   NM_152682
VERSION     NM_152682.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   3  (bases 1 to 2601)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 2601)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DA250301.1, DA766837.1, AL540937.3, AK315515.1, BC017472.1,
            AC108477.5 and BQ185399.1.
            
            On Nov 22, 2018 this sequence version replaced NM_152682.3.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: DA735086.1, SRR1660809.30080.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000326397.10/ ENSP00000388920.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-23                DA250301.1         35-57
            24-29               DA766837.1         57-62
            30-245              AL540937.3         1-216
            246-246             AK315515.1         214-214
            247-602             AL540937.3         218-573
            603-603             AK315515.1         571-571
            604-697             AL540937.3         575-668
            698-700             AK315515.1         666-668
            701-817             AL540937.3         672-788
            818-2539            BC017472.1         787-2508
            2540-2540           AC108477.5         13187-13187         c
            2541-2597           BC017472.1         2509-2565
            2598-2601           BQ185399.1         18-21               c
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4q35.1"
     gene            1..2601
                     /gene="RWDD4"
                     /gene_synonym="FAM28A; RWDD4A"
                     /note="RWD domain containing 4"
                     /db_xref="GeneID:201965"
                     /db_xref="HGNC:HGNC:23750"
     exon            1..257
                     /gene="RWDD4"
                     /gene_synonym="FAM28A; RWDD4A"
                     /inference="alignment:Splign:2.1.0"
     CDS             234..800
                     /gene="RWDD4"
                     /gene_synonym="FAM28A; RWDD4A"
                     /note="isoform 1 is encoded by transcript variant 1; RWD
                     domain containing 4A; family with sequence similarity 28,
                     member A; RWD domain-containing protein 4A"
                     /codon_start=1
                     /product="RWD domain-containing protein 4 isoform 1"
                     /protein_id="NP_689895.2"
                     /db_xref="CCDS:CCDS34111.1"
                     /db_xref="GeneID:201965"
                     /db_xref="HGNC:HGNC:23750"
                     /translation="MSANEDQEMELEALRSIYEGDESFRELSPVSFQYRIGENGDPKA
                     FLIEISWTETYPQTPPILSMNAFFNNTISSAVKQSILAKLQEAVEANLGTAMTYTLFE
                     YAKDNKEQFMENHNPINSATSISNIISIETPNTAPSSKKKDKKEQLSKAQKRKLADKT
                     DHKGELPRGWNWVDVVKHLSKTGSKDDE"
     exon            258..338
                     /gene="RWDD4"
                     /gene_synonym="FAM28A; RWDD4A"
                     /inference="alignment:Splign:2.1.0"
     exon            339..448
                     /gene="RWDD4"
                     /gene_synonym="FAM28A; RWDD4A"
                     /inference="alignment:Splign:2.1.0"
     exon            449..596
                     /gene="RWDD4"
                     /gene_synonym="FAM28A; RWDD4A"
                     /inference="alignment:Splign:2.1.0"
     exon            597..714
                     /gene="RWDD4"
                     /gene_synonym="FAM28A; RWDD4A"
                     /inference="alignment:Splign:2.1.0"
     exon            715..764
                     /gene="RWDD4"
                     /gene_synonym="FAM28A; RWDD4A"
                     /inference="alignment:Splign:2.1.0"
     exon            765..767
                     /gene="RWDD4"
                     /gene_synonym="FAM28A; RWDD4A"
                     /inference="alignment:Splign:2.1.0"
     exon            768..2601
                     /gene="RWDD4"
                     /gene_synonym="FAM28A; RWDD4A"
                     /inference="alignment:Splign:2.1.0"
     regulatory      927..932
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RWDD4"
                     /gene_synonym="FAM28A; RWDD4A"
                     /note="hexamer: ATTAAA"
     polyA_site      949
                     /gene="RWDD4"
                     /gene_synonym="FAM28A; RWDD4A"
                     /note="major polyA site"
     regulatory      2576..2581
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RWDD4"
                     /gene_synonym="FAM28A; RWDD4A"
                     /note="hexamer: AATAAA"
     polyA_site      2601
                     /gene="RWDD4"
                     /gene_synonym="FAM28A; RWDD4A"
                     /note="major polyA site"
ORIGIN      
        1 acttcccagg ccccaccagc cgcggctccg gctcgtagcc cacagcccac tgccggcggc
       61 tgggcgctgc cgaggctcgg ggcgcgcgca gttggcgtct gccagtgcca agactgtgcc
      121 gcccccacag ccgaggcgcg aaagggggac gcccggcctc tgggccgctg ccttcgcttt
      181 ctcttcgttg ttgcgaacgc cgtccgctca ggaggcgccc cgcgaccggc gcgatgagtg
      241 ccaacgagga ccaggagatg gaactagaag cattacgctc tatttatgaa ggagatgaaa
      301 gtttccggga attaagtcca gtttcttttc aatataggat aggtgaaaat ggtgatccca
      361 aagccttctt aatagagatt tcctggacag aaacatatcc ccaaacacct ccaattctat
      421 ctatgaacgc tttttttaac aacaccatat catcagctgt aaagcagagt atattagcca
      481 agctacagga agcagtagaa gctaatcttg gaaccgctat gacctataca ttgtttgaat
      541 atgccaaaga caataaagag cagttcatgg agaatcacaa tcccatcaat tccgcaacat
      601 cgataagcaa tatcatctca attgaaactc ctaatacagc cccatcaagt aagaaaaaag
      661 acaaaaaaga acaactttca aaagcccaga agcgtaagct ggcagacaaa acagatcaca
      721 aaggagaact tcctcgaggc tggaactggg ttgatgttgt gaagcattta agcaaaactg
      781 gctctaagga tgatgagtag cacttggaat ttgagacaag gaaagagcat tctttaaaga
      841 gtaaaactgg gttcaaaatc tttcattact attttctggt attgaggcga ctttttataa
      901 aacacaattt tttgtatgtt tcttacatta aaaaggttgt aagttgaaag ttcatgaaga
      961 gatcttgttg tattaaatta ttttcacaaa cttgccttaa taaaaggtga aaatgttact
     1021 gtttagtata ctttatgaag ccccttgagc tttataaatg gacaggcatg gggaataaga
     1081 atcagtgtta atttaaatga tcttatcctg gtggatgtgc tattttctta aaggagtatg
     1141 aagccctttt caaactatca tcccagtgga gcggagtact cagtgaacag ttactccata
     1201 gtgcaatcca tattaatagg cttcttctct taagtcttca tctcttcttt tgcttaatta
     1261 ctgaaccgta aattacttca gagaaattta aatgctggta tttgaacttt atacatgata
     1321 ctttttgtag tttcttttaa tttttgaaag atgaactgct tccttttaat aaattaatat
     1381 ctatttatac ttttctcttg atttgggtca agatgtttga tcatgagtgc tttgagtggt
     1441 atgtggaata ggagaatata aaaacaaatc tgccaaatac actagaaagc attttagtaa
     1501 gaaatgctgg ccctttctta aaacatttct cttgcatata ccaggatggg agtaaaagat
     1561 gccttaatat ttagtttttg tattgttgga gacattgatt ttaataaaat cctatttatc
     1621 tgctgttgtg tgcttttagt tgttggataa ctgaggtctc ctaaatggtt caacataaaa
     1681 ccacatttca agtcttgttt ctttttggag tgtcttttca agtattcaaa tgtatttctc
     1741 aacctgagca tctttttaat catatacatg ggagtctttt aaatgctgaa ctgttacaca
     1801 tgcttgattt aaaaataata ataatagagg aaactattgg tctagttgtg ccaagaaaag
     1861 tttctgatgt ttatgtgtga tgtacagtga ttttgtatat gcgcccagct ttaagaacac
     1921 ataaaactat tacgtctggt aggaagattg ttagtgcctc aagttacacc tgtgcagctt
     1981 gggtctgagt tttgatagaa cagtaaacat ttaaagaagt taagagcagt ttgagctgta
     2041 tccgcggttt ttactcgtta actgacttca gctaaatagt ttgaattata gagtaagtat
     2101 aattacagca aaggagttaa tctcattttc aaagctgttt ctcattttat ttcttgaatt
     2161 aatgtagagc aaaacatgtt aaaattcagg accactggaa tatggcaact tatgtttcag
     2221 ggttgtgtgt gggtagtatt tgtggttgta ttggtttgtt ttttgttttt ggagaaacat
     2281 ctgctagtgg aataaaatac tttgttttgc tctgaagaga ctgaaattgt tcaggcttat
     2341 tatggctcat agattacaga gaatgatgct agttacatgc caatgaacta tttttactct
     2401 ttttatatga aatgtaaaaa tttgtagggg ttctggtgat ggtggtacct cttattacct
     2461 tatgtaaaac acttgaacag cctcatcaat attgccgtca tctgtttaac actcccagta
     2521 tattttctca atgtctgttt acttaaaatt ttgtggagtg acataattaa taagcaataa
     2581 agtctgaatt atacacagcg a
//
