LOCUS       NM_006666               1545 bp    mRNA    linear   PRI 29-OCT-2024
DEFINITION  Homo sapiens RuvB like AAA ATPase 2 (RUVBL2), transcript variant 1,
            mRNA.
ACCESSION   NM_006666
VERSION     NM_006666.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Chen,D., Ji,F., Zhou,Q., Cheung,H., Pan,Y., Lau,H.C., Liang,C.,
            Yang,Z., Huang,P., Wei,Q., Cheung,A.H., Kang,W., Chen,H., Yu,J. and
            Wong,C.C.
  TITLE     RUVBL1/2 Blockade Targets YTHDF1 Activity to Suppress m6A-Dependent
            Oncogenic Translation and Colorectal Tumorigenesis
  JOURNAL   Cancer Res 84 (17), 2856-2872 (2024)
   PUBMED   38900944
  REMARK    GeneRIF: RUVBL1/2 Blockade Targets YTHDF1 Activity to Suppress
            m6A-Dependent Oncogenic Translation and Colorectal Tumorigenesis.
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Zemke,N.R., Hsu,E., Barshop,W.D., Sha,J., Wohlschlegel,J.A. and
            Berk,A.J.
  TITLE     Adenovirus E1A binding to DCAF10 targets proteasomal degradation of
            RUVBL1/2 AAA+ ATPases required for quaternary assembly of
            multiprotein machines, innate immunity, and responses to metabolic
            stress
  JOURNAL   J Virol 97 (12), e0099323 (2023)
   PUBMED   37962355
  REMARK    GeneRIF: Adenovirus E1A binding to DCAF10 targets proteasomal
            degradation of RUVBL1/2 AAA+ ATPases required for quaternary
            assembly of multiprotein machines, innate immunity, and responses
            to metabolic stress.
REFERENCE   3  (bases 1 to 1545)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   4  (bases 1 to 1545)
  AUTHORS   Goos,H., Kinnunen,M., Salokas,K., Tan,Z., Liu,X., Yadav,L.,
            Zhang,Q., Wei,G.H. and Varjosalo,M.
  TITLE     Human transcription factor protein interaction networks
  JOURNAL   Nat Commun 13 (1), 766 (2022)
   PUBMED   35140242
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1545)
  AUTHORS   Wang,Y., Zhou,J., Mackintosh,S.G. and Du,Y.
  TITLE     RuvB-Like Protein 2 Interacts with the NS1 Protein of Influenza A
            Virus and Affects Apoptosis That Is Counterbalanced by Type I
            Interferons
  JOURNAL   Viruses 13 (6), 1038 (2021)
   PUBMED   34072766
  REMARK    GeneRIF: RuvB-Like Protein 2 Interacts with the NS1 Protein of
            Influenza A Virus and Affects Apoptosis That Is Counterbalanced by
            Type I Interferons.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1545)
  AUTHORS   Ikura,T., Ogryzko,V.V., Grigoriev,M., Groisman,R., Wang,J.,
            Horikoshi,M., Scully,R., Qin,J. and Nakatani,Y.
  TITLE     Involvement of the TIP60 histone acetylase complex in DNA repair
            and apoptosis
  JOURNAL   Cell 102 (4), 463-473 (2000)
   PUBMED   10966108
REFERENCE   7  (bases 1 to 1545)
  AUTHORS   Parfait,B., Giovangrandi,Y., Asheuer,M., Laurendeau,I., Olivi,M.,
            Vodovar,N., Vidaud,D., Vidaud,M. and Bieche,I.
  TITLE     Human TIP49b/RUVBL2 gene: genomic structure, expression pattern,
            physical link to the human CGB/LHB gene cluster on chromosome
            19q13.3
  JOURNAL   Ann Genet 43 (2), 69-74 (2000)
   PUBMED   10998447
REFERENCE   8  (bases 1 to 1545)
  AUTHORS   Wood,M.A., McMahon,S.B. and Cole,M.D.
  TITLE     An ATPase/helicase complex is an essential cofactor for oncogenic
            transformation by c-Myc
  JOURNAL   Mol Cell 5 (2), 321-330 (2000)
   PUBMED   10882073
REFERENCE   9  (bases 1 to 1545)
  AUTHORS   Salzer,U., Kubicek,M. and Prohaska,R.
  TITLE     Isolation, molecular characterization, and tissue-specific
            expression of ECP-51 and ECP-54 (TIP49), two homologous,
            interacting erythroid cytosolic proteins
  JOURNAL   Biochim Biophys Acta 1446 (3), 365-370 (1999)
   PUBMED   10524211
REFERENCE   10 (bases 1 to 1545)
  AUTHORS   Kanemaki,M., Kurokawa,Y., Matsu-ura,T., Makino,Y., Masani,A.,
            Okazaki,K., Morishita,T. and Tamura,T.A.
  TITLE     TIP49b, a new RuvB-like DNA helicase, is included in a complex
            together with another RuvB-like DNA helicase, TIP49a
  JOURNAL   J Biol Chem 274 (32), 22437-22444 (1999)
   PUBMED   10428817
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC008687.5 and BC004531.2.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_006666.2.
            
            Summary: This gene encodes the second human homologue of the
            bacterial RuvB gene. Bacterial RuvB protein is a DNA helicase
            essential for homologous recombination and DNA double-strand break
            repair. Functional analysis showed that this gene product has both
            ATPase and DNA helicase activities. This gene is physically linked
            to the CGB/LHB gene cluster on chromosome 19q13.3, and is very
            close (55 nt) to the LHB gene, in the opposite orientation.
            [provided by RefSeq, Jul 2008].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189667.184676.1,
                                           SRR3476690.1073467.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000595090.6/ ENSP00000473172.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-12                AC008687.5         5195-5206
            13-1501             BC004531.2         1-1489
            1502-1545           AC008687.5         27235-27278
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19q13.33"
     gene            1..1545
                     /gene="RUVBL2"
                     /gene_synonym="CGI-46; ECP-51; ECP51; INO80J; REPTIN;
                     RVB2; TAP54-beta; TIH2; TIP48; TIP49B"
                     /note="RuvB like AAA ATPase 2"
                     /db_xref="GeneID:10856"
                     /db_xref="HGNC:HGNC:10475"
                     /db_xref="MIM:604788"
     exon            1..37
                     /gene="RUVBL2"
                     /gene_synonym="CGI-46; ECP-51; ECP51; INO80J; REPTIN;
                     RVB2; TAP54-beta; TIH2; TIP48; TIP49B"
                     /inference="alignment:Splign:2.1.0"
     CDS             26..1417
                     /gene="RUVBL2"
                     /gene_synonym="CGI-46; ECP-51; ECP51; INO80J; REPTIN;
                     RVB2; TAP54-beta; TIH2; TIP48; TIP49B"
                     /EC_number="3.6.4.12"
                     /note="isoform 1 is encoded by transcript variant 1; RuvB
                     (E coli homolog)-like 2; INO80 complex subunit J;
                     erythrocyte cytosolic protein, 51-KD; repressing pontin
                     52; 48 kDa TBP-interacting protein; TIP60-associated
                     protein 54-beta; 51 kDa erythrocyte cytosolic protein; 48
                     kDa TATA box-binding protein-interacting protein; reptin52
                     protein"
                     /codon_start=1
                     /product="ruvB-like 2 isoform 1"
                     /protein_id="NP_006657.1"
                     /db_xref="CCDS:CCDS42588.1"
                     /db_xref="GeneID:10856"
                     /db_xref="HGNC:HGNC:10475"
                     /db_xref="MIM:604788"
                     /translation="MATVTATTKVPEIRDVTRIERIGAHSHIRGLGLDDALEPRQASQ
                     GMVGQLAARRAAGVVLEMIREGKIAGRAVLIAGQPGTGKTAIAMGMAQALGPDTPFTA
                     IAGSEIFSLEMSKTEALTQAFRRSIGVRIKEETEIIEGEVVEIQIDRPATGTGSKVGK
                     LTLKTTEMETIYDLGTKMIESLTKDKVQAGDVITIDKATGKISKLGRSFTRARDYDAM
                     GSQTKFVQCPDGELQKRKEVVHTVSLHEIDVINSRTQGFLALFSGDTGEIKSEVREQI
                     NAKVAEWREEGKAEIIPGVLFIDEVHMLDIESFSFLNRALESDMAPVLIMATNRGITR
                     IRGTSYQSPHGIPIDLLDRLLIVSTTPYSEKDTKQILRIRCEEEDVEMSEDAYTVLTR
                     IGLETSLRYAIQLITAASLVCRKRKGTEVQVDDIKRVYSLFLDESRSTQYMKEYQDAF
                     LFNELKGETMDTS"
     exon            38..92
                     /gene="RUVBL2"
                     /gene_synonym="CGI-46; ECP-51; ECP51; INO80J; REPTIN;
                     RVB2; TAP54-beta; TIH2; TIP48; TIP49B"
                     /inference="alignment:Splign:2.1.0"
     exon            93..148
                     /gene="RUVBL2"
                     /gene_synonym="CGI-46; ECP-51; ECP51; INO80J; REPTIN;
                     RVB2; TAP54-beta; TIH2; TIP48; TIP49B"
                     /inference="alignment:Splign:2.1.0"
     exon            149..290
                     /gene="RUVBL2"
                     /gene_synonym="CGI-46; ECP-51; ECP51; INO80J; REPTIN;
                     RVB2; TAP54-beta; TIH2; TIP48; TIP49B"
                     /inference="alignment:Splign:2.1.0"
     exon            291..420
                     /gene="RUVBL2"
                     /gene_synonym="CGI-46; ECP-51; ECP51; INO80J; REPTIN;
                     RVB2; TAP54-beta; TIH2; TIP48; TIP49B"
                     /inference="alignment:Splign:2.1.0"
     exon            421..487
                     /gene="RUVBL2"
                     /gene_synonym="CGI-46; ECP-51; ECP51; INO80J; REPTIN;
                     RVB2; TAP54-beta; TIH2; TIP48; TIP49B"
                     /inference="alignment:Splign:2.1.0"
     exon            488..594
                     /gene="RUVBL2"
                     /gene_synonym="CGI-46; ECP-51; ECP51; INO80J; REPTIN;
                     RVB2; TAP54-beta; TIH2; TIP48; TIP49B"
                     /inference="alignment:Splign:2.1.0"
     exon            595..688
                     /gene="RUVBL2"
                     /gene_synonym="CGI-46; ECP-51; ECP51; INO80J; REPTIN;
                     RVB2; TAP54-beta; TIH2; TIP48; TIP49B"
                     /inference="alignment:Splign:2.1.0"
     exon            689..812
                     /gene="RUVBL2"
                     /gene_synonym="CGI-46; ECP-51; ECP51; INO80J; REPTIN;
                     RVB2; TAP54-beta; TIH2; TIP48; TIP49B"
                     /inference="alignment:Splign:2.1.0"
     exon            813..907
                     /gene="RUVBL2"
                     /gene_synonym="CGI-46; ECP-51; ECP51; INO80J; REPTIN;
                     RVB2; TAP54-beta; TIH2; TIP48; TIP49B"
                     /inference="alignment:Splign:2.1.0"
     exon            908..1026
                     /gene="RUVBL2"
                     /gene_synonym="CGI-46; ECP-51; ECP51; INO80J; REPTIN;
                     RVB2; TAP54-beta; TIH2; TIP48; TIP49B"
                     /inference="alignment:Splign:2.1.0"
     exon            1027..1146
                     /gene="RUVBL2"
                     /gene_synonym="CGI-46; ECP-51; ECP51; INO80J; REPTIN;
                     RVB2; TAP54-beta; TIH2; TIP48; TIP49B"
                     /inference="alignment:Splign:2.1.0"
     exon            1147..1276
                     /gene="RUVBL2"
                     /gene_synonym="CGI-46; ECP-51; ECP51; INO80J; REPTIN;
                     RVB2; TAP54-beta; TIH2; TIP48; TIP49B"
                     /inference="alignment:Splign:2.1.0"
     exon            1277..1391
                     /gene="RUVBL2"
                     /gene_synonym="CGI-46; ECP-51; ECP51; INO80J; REPTIN;
                     RVB2; TAP54-beta; TIH2; TIP48; TIP49B"
                     /inference="alignment:Splign:2.1.0"
     exon            1392..1545
                     /gene="RUVBL2"
                     /gene_synonym="CGI-46; ECP-51; ECP51; INO80J; REPTIN;
                     RVB2; TAP54-beta; TIH2; TIP48; TIP49B"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1479..1484
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RUVBL2"
                     /gene_synonym="CGI-46; ECP-51; ECP51; INO80J; REPTIN;
                     RVB2; TAP54-beta; TIH2; TIP48; TIP49B"
                     /note="hexamer: TATAAA"
     polyA_site      1501
                     /gene="RUVBL2"
                     /gene_synonym="CGI-46; ECP-51; ECP51; INO80J; REPTIN;
                     RVB2; TAP54-beta; TIH2; TIP48; TIP49B"
                     /note="major polyA site"
     polyA_site      1545
                     /gene="RUVBL2"
                     /gene_synonym="CGI-46; ECP-51; ECP51; INO80J; REPTIN;
                     RVB2; TAP54-beta; TIH2; TIP48; TIP49B"
ORIGIN      
        1 aggactctgg cagttggtga gcatcatggc aaccgttaca gccacaacca aagtcccgga
       61 gatccgtgat gtaacaagga ttgagcgaat cggtgcccac tcccacatcc ggggactggg
      121 gctggacgat gccttggagc ctcggcaggc ttcgcaaggc atggtgggtc agctggcggc
      181 acggcgggcg gctggcgtgg tgctggagat gatccgggaa gggaagattg ccggtcgggc
      241 agtccttatt gctggccagc cgggcacggg gaagacggcc atcgccatgg gcatggcgca
      301 ggccctgggc cctgacacgc cattcacagc catcgccggc agtgaaatct tctccctgga
      361 gatgagcaag accgaggcgc tgacgcaggc cttccggcgg tccatcggcg ttcgcatcaa
      421 ggaggagacg gagatcatcg aaggggaggt ggtggagatc cagattgatc gaccagcaac
      481 agggacgggc tccaaggtgg gcaaactgac cctcaagacc acagagatgg agaccatcta
      541 cgacctgggc accaagatga ttgagtccct gaccaaggac aaggtccagg ccggggacgt
      601 gatcaccatc gacaaggcga cgggcaagat ctccaagctg ggccgctcct tcacacgcgc
      661 ccgcgactac gacgctatgg gctcccagac caagttcgtg cagtgcccag atggggagct
      721 ccagaaacgc aaggaggtgg tgcacaccgt gtccctgcac gagatcgacg tcatcaactc
      781 tcgcacccag ggcttcctgg cgctcttctc aggtgacaca ggggagatca agtcagaagt
      841 ccgtgagcag atcaatgcca aggtggctga gtggcgcgag gagggcaagg cggagatcat
      901 ccctggagtg ctgttcatcg acgaggtcca catgctggac atcgagagct tctccttcct
      961 caaccgggcc ctggagagtg acatggcgcc tgtcctgatc atggccacca accgtggcat
     1021 cacgcgaatc cggggcacca gctaccagag ccctcacggc atccccatag acctgctgga
     1081 ccggctgctt atcgtctcca ccacccccta cagcgagaaa gacacgaagc agatcctccg
     1141 catccggtgc gaggaagaag atgtggagat gagtgaggac gcctacacgg tgctgacccg
     1201 catcgggctg gagacgtcac tgcgctacgc catccagctc atcacagctg ccagcttggt
     1261 gtgccggaaa cgcaagggta cagaagtgca ggtggatgac atcaagcggg tctactcact
     1321 cttcctggac gagtcccgct ccacgcagta catgaaggag taccaggacg ccttcctctt
     1381 caacgaactc aaaggcgaga ccatggacac ctcctgagtt ggatgtcatc ccccgacccc
     1441 accctgtttt ccaccagagt tctgacactg tgactctgta taaaatggtt gggaagctgc
     1501 acccaccctg tgtatgtgtg gttgccctga gcccacagaa agaca
//
