LOCUS       NM_004350               4267 bp    mRNA    linear   PRI 12-FEB-2025
DEFINITION  Homo sapiens RUNX family transcription factor 3 (RUNX3), transcript
            variant 2, mRNA.
ACCESSION   NM_004350
VERSION     NM_004350.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4267)
  AUTHORS   Wang,J., Wang,J., Zhang,J., Gong,H., Li,J., Song,Y., Huang,Y.,
            Ma,B., Gu,W. and Yang,R.
  TITLE     Association between the methylations of RUNX3 in peripheral blood
            and lung cancer: a case-control study
  JOURNAL   Biomarkers 29 (6), 343-351 (2024)
   PUBMED   38923933
  REMARK    GeneRIF: Association between the methylations of RUNX3 in
            peripheral blood and lung cancer: a case-control study.
REFERENCE   2  (bases 1 to 4267)
  AUTHORS   Zhao,B., Xia,Z., Yang,B., Guo,Y., Zhou,R., Gu,M., Liu,M., Li,Q.,
            Bai,W., Huang,J., Zhang,X., Zhu,C., Leung,K.T., Chen,C. and Dong,J.
  TITLE     USP7 promotes IgA class switching through stabilizing RUNX3 for
            germline transcription activation
  JOURNAL   Cell Rep 43 (5), 114194 (2024)
   PUBMED   38735043
  REMARK    GeneRIF: USP7 promotes IgA class switching through stabilizing
            RUNX3 for germline transcription activation.
            Erratum:[Cell Rep. 2024 Jun 25;43(6):114315. doi:
            10.1016/j.celrep.2024.114315.. PMID: 38795349]
REFERENCE   3  (bases 1 to 4267)
  AUTHORS   Wang,N., Miao,X., Lu,W., Ji,Y., Zheng,Y., Meng,D., Liu,H. and
            Xiang,C.
  TITLE     RUNX3 exerts tumor-suppressive role through inhibiting EXOSC4
            expression
  JOURNAL   Funct Integr Genomics 24 (3), 103 (2024)
   PUBMED   38913281
  REMARK    GeneRIF: RUNX3 exerts tumor-suppressive role through inhibiting
            EXOSC4 expression.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4267)
  AUTHORS   Zheng,G., Ye,H., Bai,J. and Zhang,X.
  TITLE     Downregulation of lncRNA MIR17HG reduced tumorigenicity and
            Treg-mediated immune escape of non-small-cell lung cancer cells
            through targeting the miR-17-5p/RUNX3 axis
  JOURNAL   J Biochem Mol Toxicol 38 (5), e23715 (2024)
   PUBMED   38704830
  REMARK    GeneRIF: Downregulation of lncRNA MIR17HG reduced tumorigenicity
            and Treg-mediated immune escape of non-small-cell lung cancer cells
            through targeting the miR-17-5p/RUNX3 axis.
REFERENCE   5  (bases 1 to 4267)
  AUTHORS   He,Z., Zhang,H., Xiao,H., Zhang,X., Xu,H., Sun,R. and Li,S.
  TITLE     Ubiquitylation of RUNX3 by RNA-binding ubiquitin ligase MEX3C
            promotes tumorigenesis in lung adenocarcinoma
  JOURNAL   J Transl Med 22 (1), 216 (2024)
   PUBMED   38424632
  REMARK    GeneRIF: Ubiquitylation of RUNX3 by RNA-binding ubiquitin ligase
            MEX3C promotes tumorigenesis in lung adenocarcinoma.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4267)
  AUTHORS   Levanon,D., Goldstein,R.E., Bernstein,Y., Tang,H., Goldenberg,D.,
            Stifani,S., Paroush,Z. and Groner,Y.
  TITLE     Transcriptional repression by AML1 and LEF-1 is mediated by the
            TLE/Groucho corepressors
  JOURNAL   Proc Natl Acad Sci U S A 95 (20), 11590-11595 (1998)
   PUBMED   9751710
REFERENCE   7  (bases 1 to 4267)
  AUTHORS   Bae,S.C., Takahashi,E., Zhang,Y.W., Ogawa,E., Shigesada,K.,
            Namba,Y., Satake,M. and Ito,Y.
  TITLE     Cloning, mapping and expression of PEBP2 alpha C, a third gene
            encoding the mammalian Runt domain
  JOURNAL   Gene 159 (2), 245-248 (1995)
   PUBMED   7622058
REFERENCE   8  (bases 1 to 4267)
  AUTHORS   Wijmenga,C., Speck,N.A., Dracopoli,N.C., Hofker,M.H., Liu,P. and
            Collins,F.S.
  TITLE     Identification of a new murine runt domain-containing gene, Cbfa3,
            and localization of the human homolog, CBFA3, to chromosome
            1p35-pter
  JOURNAL   Genomics 26 (3), 611-614 (1995)
   PUBMED   7607690
REFERENCE   9  (bases 1 to 4267)
  AUTHORS   Levanon,D., Negreanu,V., Bernstein,Y., Bar-Am,I., Avivi,L. and
            Groner,Y.
  TITLE     AML1, AML2, and AML3, the human members of the runt domain
            gene-family: cDNA structure, expression, and chromosomal
            localization
  JOURNAL   Genomics 23 (2), 425-432 (1994)
   PUBMED   7835892
REFERENCE   10 (bases 1 to 4267)
  AUTHORS   Bae,S.C., Yamaguchi-Iwai,Y., Ogawa,E., Maruyama,M., Inuzuka,M.,
            Kagoshima,H., Shigesada,K., Satake,M. and Ito,Y.
  TITLE     Isolation of PEBP2 alpha B cDNA representing the mouse homolog of
            human acute myeloid leukemia gene, AML1
  JOURNAL   Oncogene 8 (3), 809-814 (1993)
   PUBMED   8437866
REFERENCE   11 (bases 400 to 400)
  AUTHORS   Bae,S.C., Yamaguchi-Iwai,Y., Ogawa,E., Maruyama,M., Inuzuka,M.,
            Kagoshima,H., Shigesada,K., Satake,M. and Ito,Y.
  TITLE     Isolation of PEBP2 alpha B cDNA representing the mouse homolog of
            human acute myeloid leukemia gene, AML1
  JOURNAL   Oncogene 8 (3), 809-814 (1993)
   PUBMED   8437866
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AJ001432.1, AL023096.1,
            Z35278.1 and AI375031.1.
            
            On Jun 2, 2019 this sequence version replaced NM_004350.2.
            
            Summary: This gene encodes a member of the runt domain-containing
            family of transcription factors. A heterodimer of this protein and
            a beta subunit forms a complex that binds to the core DNA sequence
            5'-PYGPYGGT-3' found in a number of enhancers and promoters, and
            can either activate or suppress transcription. It also interacts
            with other transcription factors. It functions as a tumor
            suppressor, and the gene is frequently deleted or transcriptionally
            silenced in cancer. Alternative splicing results in multiple
            transcript variants. [provided by RefSeq, Mar 2016].
            
            Transcript Variant: This variant (2), also known as p44, contains a
            distinct 5' UTR and uses an alternate translation initiation site,
            compared to variant 1. The resulting isoform (2) has a shorter and
            distinct N-terminus when compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: Z35278.1, SRR1163658.227748.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000308873.11/ ENSP00000308051.6
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-251               AJ001432.1         3-253
            252-281             AL023096.1         65046-65075         c
            282-399             AJ001432.1         284-401
            400-2326            Z35278.1           1-1927
            2327-3857           AL023096.1         34971-36501         c
            3858-4267           AI375031.1         4-413               c
FEATURES             Location/Qualifiers
     source          1..4267
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p36.11"
     gene            1..4267
                     /gene="RUNX3"
                     /gene_synonym="AML2; CBFA3; PEBP2aC"
                     /note="RUNX family transcription factor 3"
                     /db_xref="GeneID:864"
                     /db_xref="HGNC:HGNC:10473"
                     /db_xref="MIM:600210"
     exon            1..690
                     /gene="RUNX3"
                     /gene_synonym="AML2; CBFA3; PEBP2aC"
                     /inference="alignment:Splign:2.1.0"
     CDS             409..1656
                     /gene="RUNX3"
                     /gene_synonym="AML2; CBFA3; PEBP2aC"
                     /note="isoform 2 is encoded by transcript variant 2;
                     core-binding factor, runt domain, alpha subunit 3;
                     transcription factor AML2; acute myeloid leukemia gene 2;
                     polyomavirus enhancer-binding protein 2 alpha C subunit;
                     PEA2 alpha C; PEBP2 alpha C; CBF-alpha-3; oncogene AML-2;
                     acute myeloid leukemia 2 protein; core-binding factor
                     subunit alpha-3; SL3-3 enhancer factor 1 alpha C subunit;
                     SL3/AKV core-binding factor alpha C subunit; runt related
                     transcription factor 3"
                     /codon_start=1
                     /product="runt-related transcription factor 3 isoform 2"
                     /protein_id="NP_004341.1"
                     /db_xref="CCDS:CCDS257.1"
                     /db_xref="GeneID:864"
                     /db_xref="HGNC:HGNC:10473"
                     /db_xref="MIM:600210"
                     /translation="MRIPVDPSTSRRFTPPSPAFPCGGGGGKMGENSGALSAQAAVGP
                     GGRARPEVRSMVDVLADHAGELVRTDSPNFLCSVLPSHWRCNKTLPVAFKVVALGDVP
                     DGTVVTVMAGNDENYSAELRNASAVMKNQVARFNDLRFVGRSGRGKSFTLTITVFTNP
                     TQVATYHRAIKVTVDGPREPRRHRQKLEDQTKPFPDRFGDLERLRMRVTPSTPSPRGS
                     LSTTSHFSSQPQTPIQGTSELNPFSDPRQFDRSFPTLPTLTESRFPDPRMHYPGAMSA
                     AFPYSATPSGTSISSLSVAGMPATSRFHHTYLPPPYPGAPQNQSGPFQANPSPYHLYY
                     GTSSGSYQFSMVAGSSSGGDRSPTRMLASCTSSAASVAAGNLMNPSLGGQSDGVEADG
                     SHSNSPTALSTPGRMDEAVWRPY"
     exon            691..847
                     /gene="RUNX3"
                     /gene_synonym="AML2; CBFA3; PEBP2aC"
                     /inference="alignment:Splign:2.1.0"
     exon            848..952
                     /gene="RUNX3"
                     /gene_synonym="AML2; CBFA3; PEBP2aC"
                     /inference="alignment:Splign:2.1.0"
     exon            953..1111
                     /gene="RUNX3"
                     /gene_synonym="AML2; CBFA3; PEBP2aC"
                     /inference="alignment:Splign:2.1.0"
     exon            1112..4267
                     /gene="RUNX3"
                     /gene_synonym="AML2; CBFA3; PEBP2aC"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4181..4186
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RUNX3"
                     /gene_synonym="AML2; CBFA3; PEBP2aC"
                     /note="hexamer: AGTAAA"
     polyA_site      4214
                     /gene="RUNX3"
                     /gene_synonym="AML2; CBFA3; PEBP2aC"
                     /note="major polyA site"
     regulatory      4241..4246
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RUNX3"
                     /gene_synonym="AML2; CBFA3; PEBP2aC"
                     /note="hexamer: AATATA"
     polyA_site      4267
                     /gene="RUNX3"
                     /gene_synonym="AML2; CBFA3; PEBP2aC"
ORIGIN      
        1 gccacttgat tctggaggat ttgttctggg gctgcggccg cggagtcggg gcggccgcgg
       61 gcgagcttcg gggcgggagg cggcggcagc ggcacagccc cgcgcgggcc ccgccgcggc
      121 ccaggcagcc gggacagcca cgaggggcgg ccgcacgcgg ggccgcgcgc cgaggatgcg
      181 ggactagccg ggcaggctgc gggcggccgt cgggccagcg aggcctcgca gcgggcgggc
      241 cctggcgagt agtggccggg cgccgccccc tgcgccctga ggcccgggcc ccgccgcttc
      301 tgctttcccg cttctcgcgg cagcggcggc cgaggaggcg cccgcgccgg ccgcccccgg
      361 gggaagccgc gccgtctccg cctgcccggc gccctgacgg ccgctgttat gcgtattccc
      421 gtagacccaa gcaccagccg ccgcttcaca cctccctccc cggccttccc ctgcggcggc
      481 ggcggcggca agatgggcga gaacagcggc gcgctgagcg cgcaggcggc cgtggggccc
      541 ggagggcgcg cccggcccga ggtgcgctcg atggtggacg tgctggcgga ccacgcaggc
      601 gagctcgtgc gcaccgacag ccccaacttc ctctgctccg tgctgccctc gcactggcgc
      661 tgcaacaaga cgctgcccgt cgccttcaag gtggtggcat tgggggacgt gccggatggt
      721 acggtggtga ctgtgatggc aggcaatgac gagaactact ccgctgagct gcgcaatgcc
      781 tcggccgtca tgaagaacca ggtggccagg ttcaacgacc ttcgcttcgt gggccgcagt
      841 gggcgaggga agagtttcac cctgaccatc actgtgttca ccaaccccac ccaagtggcg
      901 acctaccacc gagccatcaa ggtgaccgtg gacggacccc gggagcccag acggcaccgg
      961 cagaagctgg aggaccagac caagccgttc cctgaccgct ttggggacct ggaacggctg
     1021 cgcatgcggg tgacaccgag cacacccagc ccccgaggct cactcagcac cacaagccac
     1081 ttcagcagcc agccccagac cccaatccaa ggcacctcgg aactgaaccc attctccgac
     1141 ccccgccagt ttgaccgctc cttccccacg ctgccaaccc tcacggagag ccgcttccca
     1201 gaccccagga tgcattatcc cggggccatg tcagctgcct tcccctacag cgccacgccc
     1261 tcgggcacga gcatcagcag cctcagcgtg gcgggcatgc cggccaccag ccgcttccac
     1321 catacctacc tcccgccacc ctacccgggg gccccgcaga accagagcgg gcccttccag
     1381 gccaacccgt ccccctacca cctctactac gggacatcct ctggctccta ccagttctcc
     1441 atggtggccg gcagcagcag tgggggcgac cgctcaccta cccgcatgct ggcctcttgc
     1501 accagcagcg ctgcctctgt cgccgccggc aacctcatga accccagcct gggcggccag
     1561 agtgatggcg tggaggccga cggcagccac agcaactcac ccacggccct gagcacgcca
     1621 ggccgcatgg atgaggccgt gtggcggccc tactgaccgc cctggtggac tcctcccgct
     1681 ggaggcgggg accctaacaa ccttcaagac cagtgatggg ccggctccga ggctccgggc
     1741 gggaatggga cctgcgctcc agggtggtct cggtcccagg gtggtcccag ctggtgggag
     1801 cctctggctg catctgtgca gccacatcct tgtacagagg cataggttac cacccccacc
     1861 ccggcccggg atactgcccc cggcccagat cctggccgtc tcatcccata cttctgtggg
     1921 gaatcagcct cctgccaccc ccccggaagg acctcactgt ctccagctat gcccagtgct
     1981 gcatgggacc catgtctcct gggacagagg ccatctctct tccagagaga ggcagcattg
     2041 gcccacagga taagcctcag gccctgggaa acctcccgac ccctgcacct tcgttggagc
     2101 ccctgcatcc cctgggtcca gccccctctg catttacaca gatttgagtc agaactggaa
     2161 agtgtccccc acccccacca ccctcgagcg gggttcccct cattgtacag atggggcagg
     2221 acccagcacg ctgctggcag agatggtttg agaacacatc caagccagtc cccccagccc
     2281 agcttcccct ccgttcctaa ctgttggctt tcccccagcc gcacgggtcc caggccccag
     2341 agaagatgag tctatggcat caggttctta aacccaggaa agcacctaca gaccggctcc
     2401 tccatgcact ttaccagctc aacgcatcca ctctctgttc tcttggcagg gcgggggagg
     2461 ggggatagga ggtccccttt cccctaggtg gtctcataat tccatttgtg gagagaacag
     2521 gagggccaga tagataggtc ctagcagaag gcattgaggt gagggatcat tttgggtcag
     2581 acatcaatgt ccctgtcccc cctgggtcca gccaagctgt gccccatccc ccaagcctcc
     2641 tgggaggatc cagccaaatc ttgcgactcc tggcacacac ctgtctgtaa cctgttttgt
     2701 gctctgaaag caaatagtcc tgagcaaaaa aaaaaaaaaa acaaaaaaac aaaaaaaaaa
     2761 caaaacagtt tttaaaactg attttagaaa aagaagctta atctaacgtt ttcaaacaca
     2821 aggtctctta caggtatagt tccgtgatta tgatagctct gtgattataa gcaacatccc
     2881 cgccccctct cccccccgcg gacccccagc tgcctcctga gggtgtgggg ttattagggt
     2941 ctcaatactt tctcaagggg ctacactccc catcaggcag catcccacca gcctgcacca
     3001 caggctcccc tgggaggacg agggaaacgc tgatgagacg ctgggcatct ctcctctgtg
     3061 gctctaggac atctgtccag gaggctgggc ggaggtgggc aggatgtgag aggtggggag
     3121 tactggctgt gcgtggcagg acagaagcac tgtaaagggc tctccagccg cagctcagct
     3181 gcactgcgtt ccgaggtgaa gtcttgcccc tgaattttgc aaaatgggaa agtgggcgct
     3241 tgcccaaggg ccaggctgca tggattctca catcagagtt ctctggccct agaaaggctt
     3301 agaaaaggcg taagggaact cataaaggct agcagcatgc ggtattttaa ctttctgcct
     3361 cggcctctgt ggatgcagaa atctgcccta caaaatgctc ttcattggtt gtctctgtga
     3421 gagcactgtc cccacccaac ctgtcacaac ggccagaacc atacaccaga gacacactgg
     3481 caggttaggc agtccttctg gtgatcctat tccattccct cctgctgcgg tttctcttgg
     3541 cctgtcctca ctggaaaaac agtctccatc tcctcaaaat agttgctgac tccctgcacc
     3601 caaggggcct ctccatgcct tcttaggaag cagctatgaa tccattgtcc ttgtagtttc
     3661 ttccctcctg ttctctggtt atagctggtc ccaggtcagc gtgggaggca cctttgggtt
     3721 cccagtgccc agcactttgt agtctcatcc cagattacta acccttcctg atcctggaga
     3781 ggcagggata gtaaataaat tgctcttcct accccatccc ccatcccctg acaaaaagtg
     3841 acggcagccg tactgagtct gtaaggccca aagtgggtac agacagcctg ggctggtaaa
     3901 agtaggtcct tatttacaag gctgcgttaa agttgtacta ggcaaacaca ctgatgtagg
     3961 aagcacgagg aaaggaagac gttttgatat agtgttactg tgagcctgtc agtagtgggt
     4021 accaatcttt tgtgacatat tgtcatgctg aggtgtgaca cctgctgcac tcatctgatg
     4081 taaaaccatc ccagagctgg cgagaggatg gagctgggtg gaaactgctt tgcactatcg
     4141 tttgcttggt gtttgttttt aacgcacaac ttgcttgtac agtaaactgt cttctgtact
     4201 atttaactgt aaaatggaat tttgactgat ttgttacaat aatataactc tgagatgtgt
     4261 ggaagga
//
