LOCUS       NM_014687               9255 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens rubicon autophagy regulator (RUBCN), transcript
            variant 2, mRNA.
ACCESSION   NM_014687 XM_032901 XM_936700
VERSION     NM_014687.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9255)
  AUTHORS   Tudorica,D.A., Basak,B., Puerta Cordova,A.S., Khuu,G., Rose,K.,
            Lazarou,M., Holzbaur,E.L.F. and Hurley,J.H.
  TITLE     A RAB7A phosphoswitch coordinates Rubicon Homology protein
            regulation of Parkin-dependent mitophagy
  JOURNAL   J Cell Biol 223 (7) (2024)
   PUBMED   38728007
  REMARK    GeneRIF: A RAB7A phosphoswitch coordinates Rubicon Homology protein
            regulation of Parkin-dependent mitophagy.
REFERENCE   2  (bases 1 to 9255)
  AUTHORS   Espinoza,S., Grunenwald,F., Gomez,W., Garcia,F.,
            Abarzua-Catalan,L., Oyarce-Pezoa,S., Hernandez,M.F., Cortes,B.I.,
            Uhrig,M., Ponce,D.P., Duran-Aniotz,C., Hetz,C., SanMartin,C.D.,
            Cornejo,V.H., Ezquer,F., Parra,V., Behrens,M.I., Manque,P.A.,
            Rojas-Rivera,D., Vidal,R.L., Woehlbier,U. and Nassif,M.
  TITLE     Neuronal Rubicon Represses Extracellular APP/Amyloid beta
            Deposition in Alzheimer's Disease
  JOURNAL   Cells 11 (12), 1860 (2022)
   PUBMED   35740989
  REMARK    GeneRIF: Neuronal Rubicon Represses Extracellular APP/Amyloid beta
            Deposition in Alzheimer's Disease.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 9255)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   4  (bases 1 to 9255)
  AUTHORS   Ji,X., Lin,L., Fan,J., Li,Y., Wei,Y., Shen,S., Su,L., Shafer,A.,
            Bjaanaes,M.M., Karlsson,A., Planck,M., Staaf,J., Helland,A.,
            Esteller,M., Zhang,R., Chen,F. and Christiani,D.C.
  TITLE     Epigenome-wide three-way interaction study identifies a complex
            pattern between TRIM27, KIAA0226, and smoking associated with
            overall survival of early-stage NSCLC
  JOURNAL   Mol Oncol 16 (3), 717-731 (2022)
   PUBMED   34932879
  REMARK    GeneRIF: Epigenome-wide three-way interaction study identifies a
            complex pattern between TRIM27, KIAA0226, and smoking associated
            with overall survival of early-stage NSCLC.
REFERENCE   5  (bases 1 to 9255)
  AUTHORS   Assoum,M., Salih,M.A., Drouot,N., Hnia,K., Martelli,A. and
            Koenig,M.
  TITLE     The Salih ataxia mutation impairs Rubicon endosomal localization
  JOURNAL   Cerebellum 12 (6), 835-840 (2013)
   PUBMED   23728897
  REMARK    GeneRIF: This study demonistrated that KIAA0226 mutation impairs
            Rubicon endosomal localization
REFERENCE   6  (bases 1 to 9255)
  AUTHORS   Behrends,C., Sowa,M.E., Gygi,S.P. and Harper,J.W.
  TITLE     Network organization of the human autophagy system
  JOURNAL   Nature 466 (7302), 68-76 (2010)
   PUBMED   20562859
REFERENCE   7  (bases 1 to 9255)
  AUTHORS   Matsunaga,K., Noda,T. and Yoshimori,T.
  TITLE     Binding Rubicon to cross the Rubicon
  JOURNAL   Autophagy 5 (6), 876-877 (2009)
   PUBMED   19550146
REFERENCE   8  (bases 1 to 9255)
  AUTHORS   Matsunaga,K., Saitoh,T., Tabata,K., Omori,H., Satoh,T.,
            Kurotori,N., Maejima,I., Shirahama-Noda,K., Ichimura,T., Isobe,T.,
            Akira,S., Noda,T. and Yoshimori,T.
  TITLE     Two Beclin 1-binding proteins, Atg14L and Rubicon, reciprocally
            regulate autophagy at different stages
  JOURNAL   Nat Cell Biol 11 (4), 385-396 (2009)
   PUBMED   19270696
  REMARK    GeneRIF: Two Beclin 1 associated proteins, Atg14L and Rubicon, were
            identified.
REFERENCE   9  (bases 1 to 9255)
  AUTHORS   Zhong,Y., Wang,Q.J., Li,X., Yan,Y., Backer,J.M., Chait,B.T.,
            Heintz,N. and Yue,Z.
  TITLE     Distinct regulation of autophagic activity by Atg14L and Rubicon
            associated with Beclin 1-phosphatidylinositol-3-kinase complex
  JOURNAL   Nat Cell Biol 11 (4), 468-476 (2009)
   PUBMED   19270693
REFERENCE   10 (bases 1 to 9255)
  AUTHORS   Muzny,D.M., Scherer,S.E., Kaul,R., Wang,J., Yu,J., Sudbrak,R.,
            Buhay,C.J., Chen,R., Cree,A., Ding,Y., Dugan-Rocha,S., Gill,R.,
            Gunaratne,P., Harris,R.A., Hawes,A.C., Hernandez,J., Hodgson,A.V.,
            Hume,J., Jackson,A., Khan,Z.M., Kovar-Smith,C., Lewis,L.R.,
            Lozado,R.J., Metzker,M.L., Milosavljevic,A., Miner,G.R.,
            Morgan,M.B., Nazareth,L.V., Scott,G., Sodergren,E., Song,X.Z.,
            Steffen,D., Wei,S., Wheeler,D.A., Wright,M.W., Worley,K.C.,
            Yuan,Y., Zhang,Z., Adams,C.Q., Ansari-Lari,M.A., Ayele,M.,
            Brown,M.J., Chen,G., Chen,Z., Clendenning,J.,
            Clerc-Blankenburg,K.P., Chen,R., Chen,Z., Davis,C., Delgado,O.,
            Dinh,H.H., Dong,W., Draper,H., Ernst,S., Fu,G.,
            Gonzalez-Garay,M.L., Garcia,D.K., Gillett,W., Gu,J., Hao,B.,
            Haugen,E., Havlak,P., He,X., Hennig,S., Hu,S., Huang,W.,
            Jackson,L.R., Jacob,L.S., Kelly,S.H., Kube,M., Levy,R., Li,Z.,
            Liu,B., Liu,J., Liu,W., Lu,J., Maheshwari,M., Nguyen,B.V.,
            Okwuonu,G.O., Palmeiri,A., Pasternak,S., Perez,L.M., Phelps,K.A.,
            Plopper,F.J., Qiang,B., Raymond,C., Rodriguez,R.,
            Saenphimmachak,C., Santibanez,J., Shen,H., Shen,Y., Subramanian,S.,
            Tabor,P.E., Verduzco,D., Waldron,L., Wang,J., Wang,J., Wang,Q.,
            Williams,G.A., Wong,G.K., Yao,Z., Zhang,J., Zhang,X., Zhao,G.,
            Zhou,J., Zhou,Y., Nelson,D., Lehrach,H., Reinhardt,R., Naylor,S.L.,
            Yang,H., Olson,M., Weinstock,G. and Gibbs,R.A.
  TITLE     The DNA sequence, annotation and analysis of human chromosome 3
  JOURNAL   Nature 440 (7088), 1194-1198 (2006)
   PUBMED   16641997
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from HY003277.1, BC033615.1,
            D86979.3, AC024560.26 and AI796534.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_014687.3.
            
            Summary: The protein encoded by this gene is a negative regulator
            of autophagy and endocytic trafficking and controls endosome
            maturation. This protein contains two conserved domains, an
            N-terminal RUN domain and a C-terminal DUF4206 domain. The RUN
            domain is involved in Ras-like GTPase signaling, and the DUF4206
            domain contains a diacylglycerol (DAG) binding-like motif. Mutation
            in this gene results in deletion of the DAG binding-like motif and
            causes a recessive ataxia. Alternatively spliced transcript
            variants encoding distinct isoforms have been found for this gene.
            [provided by RefSeq, Apr 2014].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMEA1965299, SAMEA1966682
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000296343.10/ ENSP00000296343.5
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2                 HY003277.1         23-24
            3-1446              BC033615.1         1-1444
            1447-4160           D86979.3           1362-4075
            4161-6465           AC024560.26        97037-99341
            6466-6729           AI796534.1         12-275              c
            6730-9255           AC024560.26        99606-102131
FEATURES             Location/Qualifiers
     source          1..9255
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q29"
     gene            1..9255
                     /gene="RUBCN"
                     /gene_synonym="KIAA0226; RUBICON; SCAR15"
                     /note="rubicon autophagy regulator"
                     /db_xref="GeneID:9711"
                     /db_xref="HGNC:HGNC:28991"
                     /db_xref="MIM:613516"
     exon            1..250
                     /gene="RUBCN"
                     /gene_synonym="KIAA0226; RUBICON; SCAR15"
                     /inference="alignment:Splign:2.1.0"
     CDS             186..3104
                     /gene="RUBCN"
                     /gene_synonym="KIAA0226; RUBICON; SCAR15"
                     /note="isoform 2 is encoded by transcript variant 2;
                     rundataxin; baron; beclin-1 associated RUN domain
                     containing protein; RUN domain and cysteine-rich domain
                     containing, Beclin 1-interacting protein; RUN and cysteine
                     rich domain containing beclin 1 interacting protein"
                     /codon_start=1
                     /product="run domain Beclin-1-interacting and
                     cysteine-rich domain-containing protein isoform 2"
                     /protein_id="NP_055502.1"
                     /db_xref="CCDS:CCDS43195.1"
                     /db_xref="GeneID:9711"
                     /db_xref="HGNC:HGNC:28991"
                     /db_xref="MIM:613516"
                     /translation="MRPEGAGMELGGGEERLPEESRREHWQLLGNLKTTVEGLVSTNS
                     PNVWSKYGGLERLCRDMQSILYHGLIRDQACRRQTDYWQFVKDIRWLSPHSALHVEKF
                     ISVHENDQSSADGASERAVAELWLQHSLQYHCLSAQLRPLLGDRQYIRKFYTDAAFLL
                     SDAHVTAMLQCLEAVEQNNPRLLAQIDASMFARKHESPLLVTKSQSLTALPSSTYTPP
                     NSYAQHSYFGSFSSLHQSVPNNGSERRSTSFPLSGPPRKPQESRGHVSPAEDQTIQAP
                     PVSVSALARDSPLTPNEMSSSTLTSPIEASWVSSQNDSPGDASEGPEYLAIGNLDPRG
                     RTASCQSHSSNAESSSSNLFSSSSSQKPDSAASSLGDQEGGGESQLSSVLRRSSFSEG
                     QTLTVTSGAKKSHIRSHSDTSIASRGAPESCNDKAKLRGPLPYSGQSSEVSTPSSLYM
                     EYEGGRYLCSGEGMFRRPSEGQSLISYLSEQDFGSCADLEKENAHFSISESLIAAIEL
                     MKCNMMSQCLEEEEVEEEDSDREIQELKQKIRLRRQQIRTKNLLPMYQEAEHGSFRVT
                     SSSSQFSSRDSAQLSDSGSADEVDEFEIQDADIRRNTASSSKSFVSSQSFSHCFLHST
                     SAEAVAMGLLKQFEGMQLPAASELEWLVPEHDAPQKLLPIPDSLPISPDDGQHADIYK
                     LRIRVRGNLEWAPPRPQIIFNVHPAPTRKIAVAKQNYRCAGCGIRTDPDYIKRLRYCE
                     YLGKYFCQCCHENAQMAIPSRVLRKWDFSKYYVSNFSKDLLIKIWNDPLFNVQDINSA
                     LYRKVKLLNQVRLLRVQLCHMKNMFKTCRLAKELLDSFDTVPGHLTEDLHLYSLNDLT
                     ATRKGELGPRLAELTRAGATHVERCMLCQAKGFICEFCQNEDDIIFPFELHKCRTCEE
                     CKACYHKACFKSGSCPRCERLQARREALARQSLESYLSDYEEEPAEALALEAAVLEAT
                     "
     exon            251..404
                     /gene="RUBCN"
                     /gene_synonym="KIAA0226; RUBICON; SCAR15"
                     /inference="alignment:Splign:2.1.0"
     exon            405..488
                     /gene="RUBCN"
                     /gene_synonym="KIAA0226; RUBICON; SCAR15"
                     /inference="alignment:Splign:2.1.0"
     exon            489..648
                     /gene="RUBCN"
                     /gene_synonym="KIAA0226; RUBICON; SCAR15"
                     /inference="alignment:Splign:2.1.0"
     exon            649..755
                     /gene="RUBCN"
                     /gene_synonym="KIAA0226; RUBICON; SCAR15"
                     /inference="alignment:Splign:2.1.0"
     exon            756..912
                     /gene="RUBCN"
                     /gene_synonym="KIAA0226; RUBICON; SCAR15"
                     /inference="alignment:Splign:2.1.0"
     exon            913..1446
                     /gene="RUBCN"
                     /gene_synonym="KIAA0226; RUBICON; SCAR15"
                     /inference="alignment:Splign:2.1.0"
     exon            1447..1542
                     /gene="RUBCN"
                     /gene_synonym="KIAA0226; RUBICON; SCAR15"
                     /inference="alignment:Splign:2.1.0"
     exon            1543..1658
                     /gene="RUBCN"
                     /gene_synonym="KIAA0226; RUBICON; SCAR15"
                     /inference="alignment:Splign:2.1.0"
     exon            1659..1869
                     /gene="RUBCN"
                     /gene_synonym="KIAA0226; RUBICON; SCAR15"
                     /inference="alignment:Splign:2.1.0"
     exon            1870..1971
                     /gene="RUBCN"
                     /gene_synonym="KIAA0226; RUBICON; SCAR15"
                     /inference="alignment:Splign:2.1.0"
     exon            1972..2032
                     /gene="RUBCN"
                     /gene_synonym="KIAA0226; RUBICON; SCAR15"
                     /inference="alignment:Splign:2.1.0"
     exon            2033..2165
                     /gene="RUBCN"
                     /gene_synonym="KIAA0226; RUBICON; SCAR15"
                     /inference="alignment:Splign:2.1.0"
     exon            2166..2311
                     /gene="RUBCN"
                     /gene_synonym="KIAA0226; RUBICON; SCAR15"
                     /inference="alignment:Splign:2.1.0"
     exon            2312..2376
                     /gene="RUBCN"
                     /gene_synonym="KIAA0226; RUBICON; SCAR15"
                     /inference="alignment:Splign:2.1.0"
     exon            2377..2615
                     /gene="RUBCN"
                     /gene_synonym="KIAA0226; RUBICON; SCAR15"
                     /inference="alignment:Splign:2.1.0"
     exon            2616..2677
                     /gene="RUBCN"
                     /gene_synonym="KIAA0226; RUBICON; SCAR15"
                     /inference="alignment:Splign:2.1.0"
     exon            2678..2831
                     /gene="RUBCN"
                     /gene_synonym="KIAA0226; RUBICON; SCAR15"
                     /inference="alignment:Splign:2.1.0"
     exon            2832..2925
                     /gene="RUBCN"
                     /gene_synonym="KIAA0226; RUBICON; SCAR15"
                     /inference="alignment:Splign:2.1.0"
     exon            2926..9255
                     /gene="RUBCN"
                     /gene_synonym="KIAA0226; RUBICON; SCAR15"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6104..6109
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RUBCN"
                     /gene_synonym="KIAA0226; RUBICON; SCAR15"
                     /note="hexamer: AATAAA"
     polyA_site      6123
                     /gene="RUBCN"
                     /gene_synonym="KIAA0226; RUBICON; SCAR15"
                     /note="major polyA site"
     regulatory      6691..6696
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RUBCN"
                     /gene_synonym="KIAA0226; RUBICON; SCAR15"
                     /note="hexamer: AATAAA"
     polyA_site      6729
                     /gene="RUBCN"
                     /gene_synonym="KIAA0226; RUBICON; SCAR15"
     regulatory      7882..7887
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RUBCN"
                     /gene_synonym="KIAA0226; RUBICON; SCAR15"
                     /note="hexamer: AATAAA"
     polyA_site      7909
                     /gene="RUBCN"
                     /gene_synonym="KIAA0226; RUBICON; SCAR15"
     regulatory      9119..9124
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RUBCN"
                     /gene_synonym="KIAA0226; RUBICON; SCAR15"
                     /note="hexamer: AATACA"
     polyA_site      9145
                     /gene="RUBCN"
                     /gene_synonym="KIAA0226; RUBICON; SCAR15"
     regulatory      9238..9243
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RUBCN"
                     /gene_synonym="KIAA0226; RUBICON; SCAR15"
                     /note="hexamer: ATTAAA"
     polyA_site      9255
                     /gene="RUBCN"
                     /gene_synonym="KIAA0226; RUBICON; SCAR15"
ORIGIN      
        1 gtggccggga tagaagccgc agcccaggcg gcggtccctc ccgctgtcgc cgcccgggtg
       61 tagcgcatca cccggagccc accggccgca ggtgcctcct ccggccccag ggggccccgg
      121 gagccctgaa gggcgaagcg gcagggacgc ctctcttggg cgaagaggcg gcctcaccgc
      181 cccggatgcg gccggagggc gcgggaatgg agctcggagg cggcgaggag cgcctgcctg
      241 aggagagcag gagggagcac tggcagttgc tgggtaattt gaagacgacg gtggagggtt
      301 tggtatcaac caacagcccc aacgtctggt ctaagtatgg tggcttggag cggctttgca
      361 gggacatgca gagcatcctc tatcacgggc ttatccgtga ccaggcgtgc cgccgccaga
      421 cggattactg gcagttcgtg aaagacatcc ggtggctcag tccccactca gcccttcacg
      481 tggagaagtt catcagcgtg cacgagaacg accagagcag tgctgatggt gccagtgaac
      541 gtgctgttgc cgagctgtgg ctgcagcaca gcctgcagta ccactgcctc tcagcccagc
      601 tccggcccct gctcggggat agacagtata tcagaaaatt ctacacagat gctgccttcc
      661 tgctaagtga cgctcatgtc acggccatgc tgcagtgcct ggaagcagtg gaacagaaca
      721 acccccgcct cctggctcag atcgatgcgt ccatgtttgc cagaaagcac gagagcccgc
      781 tcctggtgac aaagagccag agcctgacag ccctgcccag ttccacatac acccctccaa
      841 acagctatgc tcagcattcc tactttgggt ccttctctag cctccaccaa tccgtgccca
      901 acaatggctc agagagaaga tctacttcct ttccactctc tggccctccc cggaaacctc
      961 aagaaagcag agggcacgtc tcaccagcag aggatcaaac catccaagcc cccccagttt
     1021 cagtctctgc actagccagg gattcccctt tgaccccaaa tgaaatgagc tccagtactc
     1081 tgaccagccc catagaggca tcctgggtca gcagccagaa tgattcccca ggtgatgcca
     1141 gtgaggggcc tgagtacctg gccattggca acttggaccc ccgaggccgg actgccagct
     1201 gtcagagtca cagcagcaat gccgagagca gcagttccaa tttgttctcc tccagcagct
     1261 cccagaagcc agattctgct gcctcttcct taggggacca ggaaggaggt ggggagagcc
     1321 agctgtccag tgtcctccgc aggtccagct tctcagaggg gcagacactc actgtcacca
     1381 gtggggcaaa gaaaagccac attcgctccc attcggatac cagcattgcc tccaggggag
     1441 ctccagaatc ctgcaatgat aaggcgaagt tgagaggccc tttgccctac tctggtcaaa
     1501 gcagtgaagt cagcacaccc agctctctgt acatggaata tgaaggtggt cggtacctgt
     1561 gctcagggga aggcatgttc cgaagaccat cagaaggaca gtccctcatc agctacctct
     1621 ctgagcaaga cttcggcagc tgtgccgacc tggaaaagga gaatgcccac ttcagcatct
     1681 cagagtcctt aattgctgcc atcgagctaa tgaagtgcaa catgatgagc cagtgcctag
     1741 aggaggagga agtggaagag gaagacagtg atagagagat ccaggagctg aagcagaaga
     1801 tccgccttcg gcgccagcaa atccgcacca agaacctgct ccccatgtac caggaggctg
     1861 agcacggaag ctttcgggtc acctccagca gctcccagtt cagctcacgt gattcggcac
     1921 agctctctga ctctggctct gctgatgagg ttgatgaatt tgaaatccaa gatgctgaca
     1981 tcagaaggaa cacagcctca agcagcaaat ccttcgtttc ctcccagtcc ttctcccact
     2041 gcttcctgca ctccacgtct gctgaggcgg tggccatggg gctcctgaag cagtttgagg
     2101 ggatgcagct tccagccgcc tcggagctgg agtggcttgt cccggagcat gatgcccctc
     2161 agaagctcct gcccattcct gactcactgc ccatctcacc ggatgacggg cagcacgctg
     2221 acatctacaa gctgcggatt cgtgttcgtg gcaacttgga gtgggccccg ccccggcctc
     2281 agataatttt taatgttcat ccagccccaa cgaggaaaat tgccgtggcc aagcagaatt
     2341 accgctgtgc aggatgtggc atccggactg accctgatta catcaagcga ctgcggtact
     2401 gtgagtacct gggcaagtac ttctgccagt gctgccacga gaatgcccag atggccatcc
     2461 ccagccgggt tctgcgcaag tgggacttca gcaagtacta cgtcagcaac ttctccaagg
     2521 acctgctcat taagatctgg aatgatcctc tcttcaacgt gcaggacata aacagtgccc
     2581 tctataggaa ggtcaagctg ctcaatcaag tccggctgct gcgggtccag ctgtgtcaca
     2641 tgaagaacat gttcaagact tgccgactgg ccaaggagct tctggattcc tttgacacag
     2701 tcccaggcca cctgacagag gacctccacc tgtactcact gaatgacctg actgcgacca
     2761 ggaaggggga gctggggccc cggcttgctg agctcaccag ggcaggggct acccatgtgg
     2821 agagatgcat gctctgccaa gccaaaggct tcatctgtga gttctgtcag aatgaggatg
     2881 acatcatctt tccctttgag ctccataagt gccggacctg tgaagagtgt aaagcgtgtt
     2941 accataaagc ctgcttcaag tctggaagct gtccgcgctg cgagcggctg caggcccggc
     3001 gggaggcact ggccaggcag agcctggagt cttacctgtc agactacgag gaggagcccg
     3061 cggaagcgct ggccctggaa gccgccgtcc tggaggccac ctgaagaaag cacgtgcagc
     3121 cctccctccg ggccgggtca cacctgttgc agaactgagc cactctttga aggactcgcc
     3181 ccacctgggg cttctttttt tttttttttt tttaattatc atcatctttt tttttttttt
     3241 tactgacttg tctgacgtct gtgtgcagtc agccgtcggc aggttgatgg gtccagagtc
     3301 tgtggtgaca gataatttgt aaacaccagg tgtttccatc agaactgaca tgcgggtcct
     3361 tcagtgaagc ttctagtgcc tctgtcagtg gaagagacag caagaccaag ttcttccagc
     3421 gtctgtggcc ttctcctcta ggtttcacct gcatgtcagg tatcatttcc aattttcctt
     3481 tgtttcagtt ctggagcttc tgagccaggc ctttctcaac cacctctcct gctgctgaaa
     3541 cggggatggc gttttccctc tccctgtcct ggactggggt cagactgtgc cccgaggaga
     3601 agcagcagag aataggacta cgtcatgggc atttcgtcca cttatttggg tattttgggg
     3661 gccacagaac aatcctgact atcctagact cctcagagac ctcagaggca gctgtgaatg
     3721 tccctatgtt gccgggagtt cctgtttgaa atatttgaag catagaggat gccacaagct
     3781 gactttcttc atctaccttg gtgatcttga agcaaagaac agaactgatg ctcaggccag
     3841 gctcacctgt agccttacgc cgcaagcata cgtgaggcgc cagctctgtc gctgaaggag
     3901 cgcttactca gaggagcggt cggccccctc ttggtgttaa ggtctcttag ttaacctggc
     3961 tttttggtgc aggtgtgatc tttgaagctc aggcaggtcc ctgatgccat cctaaggtga
     4021 ggacaggaac ctcacccacc atcttcttag cgtgtccctg atgactctgt cctctgttag
     4081 atggtcgttg tgcttctgag taaaagtaca acccgactcc gttctctccc cttcctgcag
     4141 cagagctggg tccttccctg gtggccgagt ctctcttgcc ttagcttctt tggtcaaagt
     4201 tggagaaaag cttcctgcta ttagtgctgt tacagaactt gacggtttgt ggatgtgagt
     4261 gtgaatgtcc ctgtgttctt gggataacaa gagcctttat gccaattatg cacttaactc
     4321 tgtgtagcct ggtaatgttt atctgttcat ttgataatgc tgattttagt gtgctgcccc
     4381 cctccccccg ttaatgtgtg ttgatggtga agtccttttg ataatgctga ttttggtgtg
     4441 ctgcctcccc cttccccccc gttaatgtgt gtgttgacag tgaagtcctt gggtggggcc
     4501 atgtgtgtgt ttgtgatgtt ccttaagttg atgcagcttc taacctctgt gaaaacactg
     4561 gtcagagtgg cttctccaag agctggcagc tctgtgaact aaagcctgca tcatttttgt
     4621 tctgggattg aattctgccc atgggcatgt cttctcatag ttgcttgctg gtaggaaaga
     4681 aatgggcgtg ggtgctgccc tggaagctga gcggaaagtt gcctgtggtt ggtggaagct
     4741 gatgagagct tgagctggcg gtaagaagga gtctcccagg gaagtgggag aggcattaag
     4801 gtgatggcca gggctgaggc tccaccagcg tgagagggaa catgtgggaa ctggcccctg
     4861 cccttgattc ctctgcctca aagttgggat ctgaaagcca tgtagggcta gaagaccctg
     4921 aggctgttct cccttctgtt catagtgaga ctcaaaaagc caagtcccag aagttctgaa
     4981 gggctgtgac tagaagtgcc caggtccttc agggagcttt aagaatgacc ccacagaact
     5041 caagtttaac taggggttag gtcccagatt cagacccagg agtttataaa aatgagctct
     5101 acttccagtt ttggtttaaa ttacacatcc aggccaggca cagtggctca cacctgtaat
     5161 cccagcactt tgggaggcca gtgcgggcgg atcatgaggt caggagtttg agaccagcct
     5221 ggccaatgtg gtgaaaccct gtctcttcca aaaatacaaa aattagctgg gcgtggtggc
     5281 acacgcctgt aatcccagct acttgggagg ctgaggcagg agaatcgctt gaacctggga
     5341 ggcagaggtt gcagtgagcc gagattgcgc caccgcactc cagcctgggt gacagagtga
     5401 gactccgtct caaaaaacaa aaaggtgaca catccagctc tttctccagg tcactgcgct
     5461 ggaggacaga tgtgccgtct tgtcctgcct gtttcacatc agcataggat caaaggatga
     5521 caatgctgac agcttctgaa gccgaactca acagtctcat aggctcctca cttgtcactt
     5581 atttttccct agctccctca accgcacccc atccctttag atcgtgcgtc tgttttagtg
     5641 actctgacac gatgccgtcc tcaccttcca aatacccagt tatttattca agagggggga
     5701 agtgggtaga ggatgggatg ttttggaagc actttgcaag ttaccactat ctgaaaatcc
     5761 cctgctgttg cggggagaag ctttgaatgc actgaagaga attccttcta aatgaaggca
     5821 ggtgatagtg ttctttctgt aagtaaaggg aaagaaaaaa aacatagttt gcttaccagg
     5881 tggagacaag attcaagaca tagcagaaga gtggaagaca aatattttcc acttaaatga
     5941 ggctgttttt gacgttctct gccaaggatt tagagctttc gttgaactaa cataaaagga
     6001 gtgcgagtct tagtagagat gttccgtgtg tgccgcccgt gctctgaact gcgtttccac
     6061 ctgctgtggt gcttgtgcag cctggcagtt cattgtcatc tttaataaac taaggaaata
     6121 aaattctgtc tttcgtcagc ctgccttgac tgtcaagaaa tggcatgtgt ccactgtaaa
     6181 gcaggtatat ctgaacattg agaaggtttc tgtgttataa tgtaaagcaa actggaccac
     6241 acacatcaag gaaagtgagg tggagtggct gtgtctctaa cttgccatga gacctcgagc
     6301 gaaccacttc acttctctgg gtttctggtg catggtgtaa ctgtcactta aaatgaggtg
     6361 tcttcctctc cgcaacatct gacttgcctt agatgcttca gttcccactt ctgtgagcag
     6421 ctagcatctt tctcttctgc tcctggccca ctggagctcc agctctacgc tgcaactctc
     6481 tcaacatttc cccttaaatg tccccttgtc atctccaacc agtgtgtcta aaaccagcat
     6541 aaattccgta tttccaaaaa cctaaccatt agtcacccag gtttgaaaat taattgtctt
     6601 caacttcctc tgtaagtggc acactgagct catcacgcat cgtctcattc ctactccctg
     6661 tcatcaagtt ctgtggatgt tttcctctaa aataaatctc tcattacttt tctgccacta
     6721 gcacacaaat tcaggtgttc ttgatcatac attattttcc tccctgcgcc tcagtggaac
     6781 aatgattaga agcaccttgt gggatgagga gccgggcgcg gtggctcacg agtgtcatcc
     6841 agtactttga gagaccaagg cagggggatc acctgaggtc aggaattcaa gaccagcctg
     6901 gctaacatca caaaacctca tctctacaaa aatataaaaa ttagccaggc atggtggcat
     6961 gtgcttgtaa tcccagctac tcgggaggct gaatcatgag aatcacttga acctggaagg
     7021 cgagggctgc agtgagccaa gcttgctcca ttgcactcca tcctggatga agagcgagac
     7081 cctgtctcaa aaaaacaaaa caaaacaaaa aaagcaccag cacactgtgg catgaggggc
     7141 gggattgtga ccacctctac ctcccctctt agtgctcacc aagtctctgc agtagtgagc
     7201 gtgtcctcat ctcctgtctt ctccctcagc tactttgtct gcaactgctc ctgttgtgtt
     7261 tgtccagcag tcatcatcag aggacacaca tcccaaatgt ccttcagtca ctttattatt
     7321 aaactgccag ctctttcctt gccagggact aaaccaggag ataagatgat atttatatgc
     7381 tgtaaacaca ccctacatat ttaaaaatct tcatatttac aaattaaact gagaggagaa
     7441 tgaaaaagcc atgtgaaaga ctgctgaagt tgataaaatg ttcaaatgtt tttcaactag
     7501 agataggaaa atagcttaca gtacaaaacc ttcactttgt tctaaggccc ttttgttcat
     7561 tgttacaaac tggtgtagtt cacaggaagc caaagcttga tttccagtaa gttcccaaac
     7621 ttctgttctc aacatttaac ttagatttta gatgatcata ttccaaaatt ggaaagactg
     7681 ttgaagagga aaacaaggac cattgattca caatggcagc ccagcattta tttattcatt
     7741 caatcatccg tcaatcccaa acatctatag atgcctccgt gttccagaca ccatgccaga
     7801 gctgagagta caaagaatca cagaatattg tttaaagatg tttactgcag cataatttat
     7861 gaaagtaaaa agttaatgga aaataaaaaa attgatgcaa ccatcaaaat gacagttata
     7921 aaaattatga tagtgagccg cacacagtgg ctcacgcctg taatcccagc actttgggag
     7981 gccgaggcgg gcggatcacg aggtcaagag attgggacca tcctggccaa catggtgaaa
     8041 ccttgtctct actaaaaata caaaagctag ttgggcatga tggcgggcgc ctgtaatccc
     8101 agctactcgc gaggttgaga caggagaatt gcttgaaccc gggtggcgga ggttgcagtg
     8161 agccgagatc acgccactgc actccaccct gggcaacaga gtgggactct gtctcaaaca
     8221 aaaaaataaa ttatgatagt aacatgggga aaaagtcttg taatacaatg ttatgtgaga
     8281 agaagttaga aaaagtgtat gtcaaatata tatttattag tccatacata cataaataaa
     8341 tgaccaaata aataaattct cacagaagaa ttctaaacaa aatacgtaaa ttctcccagg
     8401 aggtggagtt taattccttc tcccattgag ggtggactgg aattagtgat tcactttttt
     8461 aaataaaagg atatagaaag aggaaaatag caactttaga gtagagagag ctaacacgac
     8521 cttaaccaag taatcaacgt taacatctcc aaagatgtca ttggatgtca tgtacctact
     8581 aatacaatga aaggggtaca tcttccctga cattcattca gaaacccata acctcagtct
     8641 aatcatgaga aaaacaacaa acaacataaa cacaggagca aaattcgagg aactttctac
     8701 aaaacacttg accctactca aaattgccaa ggtctcgaaa acaaggaaag agatgccgtc
     8761 acggacgaga ggagactaag cagatacgat gactgaatgc catgtgggat cccgggtggg
     8821 atcccgaaat ggagaaagga tattagtgaa aaaactggca aaatctgaat acaatttgga
     8881 gtttagttaa aagtaacata ccaatgttgg ttccttagtt ttgggaaatg tccagtacta
     8941 cagtaatgta agatggtaac attagggaga gctgaaatcg ggtgagggct atgtgacgaa
     9001 tttgtactat ctttgcaact ttactgtgga tctaaaatta ttccagatta agaagtttac
     9061 ctatataaaa agcatatgta catcataatt acagttatgc aaaaatatgg ttagaaaaaa
     9121 tacataagga aaacatacgt aaaaattagt gtggttctgt tagaatggtg gcattgtgga
     9181 gtattttttc ctcttatctg cttactgaat tagctgtaaa tgagtcattt tccataaatt
     9241 aaagagttaa tttta
//
