LOCUS       NM_173630               7830 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens rotatin (RTTN), transcript variant 1, mRNA.
ACCESSION   NM_173630
VERSION     NM_173630.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7830)
  AUTHORS   Chou,E.J. and Tang,T.K.
  TITLE     Human Microcephaly Protein RTTN Is Required for Proper Mitotic
            Progression and Correct Spindle Position
  JOURNAL   Cells 10 (6), 1441 (2021)
   PUBMED   34207628
  REMARK    GeneRIF: Human Microcephaly Protein RTTN Is Required for Proper
            Mitotic Progression and Correct Spindle Position.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7830)
  AUTHORS   Zakaria,M., Fatima,A., Klar,J., Wikstrom,J., Abdullah,U., Ali,Z.,
            Akram,T., Tariq,M., Ahmad,H., Schuster,J., Baig,S.M. and Dahl,N.
  TITLE     Primary microcephaly, primordial dwarfism, and brachydactyly in
            adult cases with biallelic skipping of RTTN exon 42
  JOURNAL   Hum Mutat 40 (7), 899-903 (2019)
   PUBMED   30927481
  REMARK    GeneRIF: Study reports on a consanguineous family with three adult
            members with primary microcephaly, developmental delay, primordial
            dwarfism, and brachydactyly segregating a homozygous splice site
            variant NM_173630.3:c.5648-5T>A in RTTN. The variant RTTN allele
            results in a nonhypomorphic skipping of exon 42 and a frameshift
            [(NP_775901.3:p.Ala1883Glyfs*6)].
REFERENCE   3  (bases 1 to 7830)
  AUTHORS   Cavallin,M., Bery,A., Maillard,C., Salomon,L.J., Bole,C.,
            Reilly,M.L., Nitschke,P., Boddaert,N. and Bahi-Buisson,N.
  TITLE     Recurrent RTTN mutation leading to severe microcephaly,
            polymicrogyria and growth restriction
  JOURNAL   Eur J Med Genet 61 (12), 755-758 (2018)
   PUBMED   30121372
  REMARK    GeneRIF: We identified, by trio based whole exome sequencing, a
            homozygous missense mutation in the RTTN gene (c.2953A>G;
            p.(Arg985Gly)) in one Moroccan patient from a consanguineous
            family.
REFERENCE   4  (bases 1 to 7830)
  AUTHORS   Stouffs,K., Moortgat,S., Vanderhasselt,T., Vandervore,L., Dica,A.,
            Mathot,M., Keymolen,K., Seneca,S., Gheldof,A., De Meirleir,L. and
            Jansen,A.C.
  TITLE     Biallelic mutations in RTTN are associated with microcephaly, short
            stature and a wide range of brain malformations
  JOURNAL   Eur J Med Genet 61 (12), 733-737 (2018)
   PUBMED   29883675
  REMARK    GeneRIF: We here report three individuals from two unrelated
            families with novel mutations in the RTTN gene. The phenotype
            consisted of microcephaly, short stature, pachygyria or
            polymicrogyria, colpocephaly, hypoplasia of the corpus callosum and
            superior vermis. These findings provide further confirmation of the
            phenotype related to pathogenic variants in RTTN.
REFERENCE   5  (bases 1 to 7830)
  AUTHORS   Sydor,A.M., Coyaud,E., Rovelli,C., Laurent,E., Liu,H., Raught,B.
            and Mennella,V.
  TITLE     PPP1R35 is a novel centrosomal protein that regulates centriole
            length in concert with the microcephaly protein RTTN
  JOURNAL   Elife 7, e37846 (2018)
   PUBMED   30168418
  REMARK    GeneRIF: Data show that PPP1R35 acts downstream of, and forms a
            complex with, RTTN, a microcephaly protein required for distal
            centriole elongation.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 7830)
  AUTHORS   Shamseldin,H., Alazami,A.M., Manning,M., Hashem,A., Caluseiu,O.,
            Tabarki,B., Esplin,E., Schelley,S., Innes,A.M., Parboosingh,J.S.,
            Lamont,R., Majewski,J., Bernier,F.P. and Alkuraya,F.S.
  CONSRTM   Care4Rare Canada Consortium
  TITLE     RTTN Mutations Cause Primary Microcephaly and Primordial Dwarfism
            in Humans
  JOURNAL   Am J Hum Genet 97 (6), 862-868 (2015)
   PUBMED   26608784
  REMARK    GeneRIF: RTTN mutations cause primary microcephaly and primordial
            dwarfism in humans.
REFERENCE   7  (bases 1 to 7830)
  AUTHORS   Toro,C., Olive,M., Dalakas,M.C., Sivakumar,K., Bilbao,J.M.,
            Tyndel,F., Vidal,N., Farrero,E., Sambuughin,N. and Goldfarb,L.G.
  TITLE     Exome sequencing identifies titin mutations causing hereditary
            myopathy with early respiratory failure (HMERF) in families of
            diverse ethnic origins
  JOURNAL   BMC Neurol 13, 29 (2013)
   PUBMED   23514108
  REMARK    Publication Status: Online-Only
REFERENCE   8  (bases 1 to 7830)
  AUTHORS   Kheradmand Kia,S., Verbeek,E., Engelen,E., Schot,R., Poot,R.A., de
            Coo,I.F., Lequin,M.H., Poulton,C.J., Pourfarzad,F., Grosveld,F.G.,
            Brehm,A., de Wit,M.C., Oegema,R., Dobyns,W.B., Verheijen,F.W. and
            Mancini,G.M.
  TITLE     RTTN mutations link primary cilia function to organization of the
            human cerebral cortex
  JOURNAL   Am J Hum Genet 91 (3), 533-540 (2012)
   PUBMED   22939636
  REMARK    GeneRIF: RTTN mutations therefore link aberrant ciliary function to
            abnormal development and organization of the cortex in human
            individuals.
REFERENCE   9  (bases 1 to 7830)
  AUTHORS   Stevens,N.R., Dobbelaere,J., Wainman,A., Gergely,F. and Raff,J.W.
  TITLE     Ana3 is a conserved protein required for the structural integrity
            of centrioles and basal bodies
  JOURNAL   J Cell Biol 187 (3), 355-363 (2009)
   PUBMED   19948479
REFERENCE   10 (bases 1 to 7830)
  AUTHORS   Faisst,A.M., Alvarez-Bolado,G., Treichel,D. and Gruss,P.
  TITLE     Rotatin is a novel gene required for axial rotation and left-right
            specification in mouse embryos
  JOURNAL   Mech Dev 113 (1), 15-28 (2002)
   PUBMED   11900971
  REMARK    GeneRIF: Study characterizing mouse rotatin gene.
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK096404.1, BF507420.1,
            BC047602.1, BC046222.2 and AC011930.5.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 17, 2019 this sequence version replaced NM_173630.3.
            
            Summary: This gene encodes a large protein whose specific function
            is unknown. Absence of the orthologous protein in mouse results in
            embryonic lethality with deficient axial rotation, abnormal
            differentiation of the neural tube, and randomized looping of the
            heart tube during development. In human, mutations in this gene are
            associated with polymicrogyria with seizures. In human fibroblasts
            this protein localizes at the ciliary basal bodies. Given the
            intracellular localization of this protein and the phenotypic
            effects of mutations, this gene is suspected of playing a role in
            the maintenance of normal ciliary structure which in turn effects
            the developmental process of left-right organ specification, axial
            rotation, and perhaps notochord development. [provided by RefSeq,
            Jan 2013].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMEA1965299, SAMEA1966682
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000640769.2/ ENSP00000491507.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-172               AK096404.1         43-214
            173-405             BF507420.1         15-247
            406-756             AK096404.1         448-798
            757-1053            AK096404.1         802-1098
            1054-5310           BC047602.1         1-4257
            5311-5829           BC046222.2         548-1066
            5830-6379           BC047602.1         4777-5326
            6380-7057           BC046222.2         1617-2294
            7058-7830           AC011930.5         102343-103115
FEATURES             Location/Qualifiers
     source          1..7830
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="18"
                     /map="18q22.2"
     gene            1..7830
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /note="rotatin"
                     /db_xref="GeneID:25914"
                     /db_xref="HGNC:HGNC:18654"
                     /db_xref="MIM:610436"
     exon            1..60
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     CDS             30..6710
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /note="isoform 1 is encoded by transcript variant 1"
                     /codon_start=1
                     /product="rotatin isoform 1"
                     /protein_id="NP_775901.3"
                     /db_xref="CCDS:CCDS42443.1"
                     /db_xref="GeneID:25914"
                     /db_xref="HGNC:HGNC:18654"
                     /db_xref="MIM:610436"
                     /translation="MVLAGLIRKLGHQLAEIRERALKSILCKIEHNLICYADLIQERQ
                     LFLHLLEWFNFPSVPMKEEVLNLLSRLVKYPPAVQHLVDVGAVEFLSKLRSNVEPNLQ
                     AEIDGILDGLFLLPSEVPALSSASYQTNQTELSKNPEILTGYFPQDKSNFQQMEVPPR
                     PVVNQTVKCLKFSTFPWLPLTTTDRHVLSSNESSLRSSNHTLIWNTCELLKDVIMQDF
                     PAEIFLQRPKIVQSLLSLLKLAFGDGKHRLALQSVSCLQQLCMYLRNRLNFHRDPGFF
                     SNKHDTVSQNSSLSYCHEARGTHHSQNPSPGSSSPRPSVVGRTGQRPRGDGQDWDAAS
                     SSGSSSHAHVNSRISVHSPLDMGHIDLPELETEDTLELQFQQLSLPQFCVSILESAVP
                     LLRTGSRQVIIRVLELLTEDMTLIGEAISTDIWDDSSLFGIDMKEKLLLVLGALGETM
                     CYHKSSISLEQPEVMLVHHRMAFISISLFAVRLLQTLLPVEKASEFLSEPMSTALFLL
                     SLDMPISLEYPNIHEAVVAYLEQLNSENYSIYKRTAEAVYSIECTCNFLSDIGKEGEK
                     NLLELVELADQALRSFSYHQHFPLIKEIISICSKIWKSAQASPLLQGESQKVLLHMLS
                     HPLPRVKAETYHCCLEITKECLGVHNVTKPVSSLCNGIHFLLHPKVLYEISVFGIQEP
                     ESEVNTAAKAILLYLLQGRLMMTALTWNKFIESLCPVIPILQGYADTEDPLGNCILLL
                     SKASSDTEEMLPCTTRLKSMLRLLLVKKPSVRSLALKLLAFHLTSEEGADTKRPLIDA
                     RVLSRVTDLFIGKKPIELRLDDRRELVIKLETVEKVYEIFTSDDVDLVLRKSAAEQLA
                     VIMQDIKMHAVVKKLCLIDKIIEYLNECVSQDGKVVECLVQPCLTLLRKVLCGDPVMR
                     VSLSQQSSLLTVLFRVSLIFHEDCSVVTEVGALFCLLLFDEVSRMDMWSVNPSNKPSL
                     PSVFSLPVSVFRRYHLPVHVIGHHAVSPYSIVLPLSADCLALKPVSDMLRIAWNLSWY
                     HGSDNLLKQMNSETKTQEILDALKLSTEDILTLKITHMASGLQDCLHSIVQAATHREV
                     RAAVTRMSFYLLNDRLSLKGCPGPCGVTLKSLAWHTALNRFLQVLPACTEDEKLLIDI
                     IHFLNKLIKEQRKNSSLELLNWILELLLRHSANPLLDLLVLTESQAREETDDIRTAVR
                     QQLQKELIALFDTLLLNFMEVTDRKCSELLYVFQTQLALKLLQCLKVTDAPHFYGLPS
                     LERTLRGMANLTAFPGWSSHSPLTKPLDICVKYLSGLLEVITSFYVERGGNAMSFMGK
                     GVTKSTILCLLHLSHEMMAQAGSLEWMSLWFLPLGSHSEEHIPTQQGLAWLIPLWVDR
                     DPEVRFTSLGLGSALTTLETGCVALANSCQNISGGLWGTVVNILLDQSECSMVRREAA
                     FILQNLLVIPMPTEIIKDYTWQGPCVHDEDSGLSLIGKPALQALLYHCHFYEHLNQMV
                     KHCYLGRCMFDLNFSAFDRNSESNDLNGLDDSFKFWRAPSRTSQDRDPSSLSTSETTV
                     APSLGSTEFQPLVQSTTLLPEASHDQFVAQGHQESTSPRPPHDSSLSAPLPKLCVFVT
                     PSLLSAMCSLLDNLLTIAPRDTAKAFRQAHLIELLCSIADATLIQTCVQELRALLPSS
                     PPAEHTQAQVSFLLEYLSSLSRLLQSCLLVEPDLVIQDELVKPLITNIIGILTICTKD
                     VLDKELISAFYHTWTHLFNLLAMLLRKAGAITLPFVTVALAKHWTAAIDMFCTCAGLS
                     ATCPALYTASLQFLSVLLTEEAKGHLQAKSKTHLCCSPTVASLLDDSQENQKSLEQLS
                     DVILQCYEGKSSKDILKRVAANALMSLLAVSRRAQKHALKANLIDNCMEQMKHINAQL
                     NLDSLRPGKAALKKKEDGVIKELSIAMQLLRNCLYQNEECKEAALEAHLVPVLHSLWP
                     WILMDDSLMQISLQLLCVYTANFPNGCSSLCWSSCGQHPVQATHRGAVSNSLMLCILK
                     LASQMPLENTTVQQMVFMLLSNLALSHDCKGVIQKSNFLQNFLSLALPKGGNKHLSNL
                     TILWLKLLLNISSGEDGQQMILRLDGCLDLLTEMSKYKHKSSPLLPLLIFHNVCFSPA
                     NKPKILANEKVITVLAACLESENQNAQRIGAAALWALIYNYQKAKTALKSPSVKRRVD
                     EAYSLAKKTFPNSEANPLNAYYLKCLENLVQLLNSS"
     exon            61..248
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            249..426
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            427..516
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            517..607
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            608..722
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            723..870
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            871..1036
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            1037..1218
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            1219..1334
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            1335..1505
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            1506..1718
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            1719..1831
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            1832..1958
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            1959..2084
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            2085..2201
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            2202..2338
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            2339..2510
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            2511..2610
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            2611..2699
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            2700..2817
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            2818..2914
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            2915..2983
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            2984..3172
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            3173..3412
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            3413..3557
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            3558..3712
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            3713..3932
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            3933..4061
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            4062..4172
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            4173..4331
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            4332..4403
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            4404..4593
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            4594..4682
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            4683..4776
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            4777..4969
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            4970..5060
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            5061..5214
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            5215..5352
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            5353..5570
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            5571..5676
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            5677..5774
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            5775..5852
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            5853..5979
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            5980..6182
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            6183..6450
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            6451..6554
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            6555..6624
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            6625..7830
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7029..7034
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /note="hexamer: AATAAA"
     polyA_site      7057
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /note="major polyA site"
     regulatory      7417..7422
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /note="hexamer: AATAAA"
     polyA_site      7437
                     /gene="RTTN"
                     /gene_synonym="MSSP"
ORIGIN      
        1 ctccggcggc tgcagattga cgggacgaga tggtcctggc agggctcatc aggaaactcg
       61 gtcatcagct ggccgagatc agggagcgcg ctctcaagag tattctctgc aagattgagc
      121 acaacttaat ctgctacgct gatctcattc aggagaggca actttttctt catttgctgg
      181 aatggttcaa tttcccgtcc gttccgatga aggaagaggt tctgaacctg ttaagcagat
      241 tggttaagta tcccccagca gtccaacatt tggttgacgt tggtgcagta gagttcttat
      301 ctaagcttcg gtctaatgtg gagccaaatc tgcaggctga aattgatggc attctggatg
      361 gactttttct tcttccttcg gaagttcctg cactatcttc tgcctcatac caaaccaatc
      421 aaactgaatt gtcaaaaaac cctgaaatct taacaggata ttttccccaa gacaaaagta
      481 atttccagca gatggaagtg ccgccacgac cagtggtaaa tcagactgtg aagtgcttga
      541 agttttctac atttccttgg ctacccctga ccaccacaga cagacatgtc ctctcctcta
      601 atgaaagctc tttaagaagt agtaaccaca ctttaatctg gaacacctgt gaactattga
      661 aggatgttat catgcaagat tttcctgctg agattttcct tcaaaggcca aaaattgttc
      721 agagcctttt atctctgttg aaactggcct ttggagatgg aaagcatcgc ctggcattac
      781 agtcggtgtc ctgcctgcag cagctgtgca tgtatttaag aaacagactt aactttcacc
      841 gagatccagg ttttttctcc aataaacacg acacagtttc ccaaaattct tctttgtctt
      901 attgtcatga agcaagaggt actcatcatt cccagaatcc ttccccagga agcagcagcc
      961 ctcggccttc tgtggttggg cgcacaggcc agcgacccag aggagatggc caggactggg
     1021 atgcagcgtc ctctagtgga agtagtagtc atgctcatgt aaactccagg atatccgttc
     1081 attcaccttt ggatatggga cacatagatc tgccagagct ggaaactgaa gacacattgg
     1141 aactacaatt ccagcagctc agtcttcccc agttttgtgt ctccattctg gaatcagctg
     1201 ttcctctctt aagaacaggt agcagacaag tgataataag agttctggaa ttgcttacag
     1261 aggatatgac acttattggt gaagcaattt caacagatat ctgggatgac agcagccttt
     1321 ttggtataga tatgaaggag aaactactcc tggtgttggg agcccttgga gaaaccatgt
     1381 gctaccataa aagtagtatc agtttggagc agccagaggt gatgcttgtg caccacagaa
     1441 tggcctttat cagcatttcc ctgtttgcag ttcgactgct acaaacgctt ctccctgttg
     1501 aaaaggcaag cgagttttta tcagagccta tgtcaacagc attatttctc ctttctttgg
     1561 acatgcctat ttctttggaa tatccaaata ttcatgaagc tgttgtggcc tatttggaac
     1621 agctgaattc tgaaaactac agtatttata aacgaactgc agaggccgtt tattcaattg
     1681 aatgcacctg taacttcctg tctgatattg ggaaagaggg agagaagaat ctcttagaat
     1741 tagtggagct ggcagaccaa gccttgcgta gcttttccta tcatcagcat ttcccgctaa
     1801 taaaggaaat catcagcatt tgttcaaaga tctggaaatc tgctcaggcc agtccattac
     1861 tacaaggaga aagtcagaag gtgcttctcc atatgttgtc tcacccattg ccacgagtga
     1921 aagctgaaac ttaccactgc tgtctggaaa tcacgaagga atgtttaggt gtccataatg
     1981 tcactaaacc cgtgtcttca ctttgcaatg gaattcattt tctacttcat ccaaaagttc
     2041 tgtatgaaat ctctgtattt ggcattcaag agcccgaaag tgaggtgaac actgctgcca
     2101 aggccattct gttatacctg cttcagggac gattgatgat gacagcattg acctggaaca
     2161 agtttattga atctctctgt cctgtcatcc caatactaca gggctatgcc gacacagaag
     2221 atcctctggg taactgcatt ctccttctaa gcaaagcaag ttctgacaca gaagagatgc
     2281 ttccgtgtac tacccgatta aagtccatgc tgcgactttt gctagtgaaa aagccatcgg
     2341 tccgttcgct agcattaaag cttttagcat tccatcttac cagtgaagaa ggggctgata
     2401 caaagcgtcc tctaatagac gccagagttc tctccagagt tactgattta ttcattggga
     2461 agaaacctat tgaactcaga ctggatgaca gaagagagct ggttattaag ttggagactg
     2521 ttgaaaaggt atatgaaatc ttcacctcag atgatgttga tctcgttttg agaaagtcag
     2581 ctgctgaaca gttagctgtg attatgcaag atattaaaat gcatgctgtg gtgaaaaagt
     2641 tatgcttaat tgacaaaata attgagtatt taaatgaatg tgtgagtcaa gatggcaagg
     2701 ttgtagaatg tttggtacaa ccatgcctca cactcttgag gaaggtttta tgtggtgatc
     2761 cagtcatgcg tgtttcgctc tcgcaacagt cttctctttt gaccgtgtta ttcagagttt
     2821 ccttaatatt tcatgaagat tgtagtgtcg tgactgaagt tggagcacta ttttgtcttc
     2881 tattatttga tgaagtatcg agaatggata tgtggtctgt taatccttcc aataaacctt
     2941 ctttgccatc ggtcttcagt ttgcctgttt ccgtttttag aaggtaccat ctacctgttc
     3001 atgtaattgg acaccatgct gtgagtcctt actccatagt tttgccctta tctgctgatt
     3061 gtttggcctt gaagccggtg tcagatatgc tgagaatagc ttggaacctg tcatggtatc
     3121 atgggagtga taatctgttg aagcaaatga actctgaaac taaaacgcaa gaaattttag
     3181 atgcattgaa gttatctaca gaggacatcc tcactctgaa gataacacac atggctagtg
     3241 ggctgcagga ctgcctccat tccattgttc aggctgcaac ccacagggaa gttagggctg
     3301 ctgtcaccag gatgagtttt tatcttctga atgacagatt gtctttaaag ggttgccctg
     3361 gtccatgtgg ggttaccttg aagtccttgg cttggcacac cgcactaaac aggtttttac
     3421 aggtgcttcc tgcttgcact gaagatgaga aactgctaat agatatcata cattttttaa
     3481 ataaattaat aaaggaacaa agaaaaaatt cttcactaga acttctaaac tggattctcg
     3541 aattacttct tagacatagt gcaaacccac tgttagacct cttggttctg acagagtcac
     3601 aggcacgaga agaaacagat gatatccgga ctgctgtcag gcaacaactt cagaaagaac
     3661 tgattgctct ttttgatacc ttgctgctca atttcatgga agttactgac aggaaatgct
     3721 cggaacttct ttacgttttt caaacgcagc tggctctgaa actgctccag tgtctgaaag
     3781 tgacggatgc gcctcatttc tatggcctgc cgtcccttga gcggacctta cgagggatgg
     3841 ctaacctcac tgcgtttccg ggatggagct cacactctcc tctcacaaag cctctagata
     3901 tctgtgtgaa gtacttgtca ggtctccttg aggtcattac ttctttttat gtggagcgtg
     3961 gaggaaatgc tatgtccttc atgggaaaag gtgttacaaa gagcacaatt ctttgcttgc
     4021 ttcacttatc ccatgagatg atggcccagg ctgggagctt ggagtggatg tcactttggt
     4081 tcttgccttt gggtagtcat agtgaagaac atattcctac tcaacaagga ttggcttggt
     4141 tgattccatt atgggttgat cgggacccag aggtgagatt cacttcactg ggattaggat
     4201 cagcactgac cacccttgaa acgggctgtg tggccttagc aaacagttgt cagaacattt
     4261 ccggtgggct ctggggaact gtggtgaaca ttcttctgga ccagtcagaa tgtagtatgg
     4321 tgcgccggga ggcggcattt attcttcaga atctccttgt aattccaatg cctacagaaa
     4381 ttataaagga ttatacttgg cagggtccct gtgttcatga tgaggactct ggcctatcgc
     4441 tcattggaaa acctgccctt caggctcttt tatatcactg ccatttttat gaacatttga
     4501 atcagatggt aaagcattgt tacctaggac ggtgtatgtt tgatttgaat ttttctgctt
     4561 ttgatagaaa ttcagaaagc aatgatttaa atggtttaga tgactcattc aagttttgga
     4621 gggctccatc taggacaagt caggatcgag atccaagttc tctctccacc tcagaaacaa
     4681 cggtggcacc ttcattgggg agtactgaat ttcagccact tgtgcagtca acaacacttc
     4741 tacctgaagc ctcccatgac cagtttgtgg ctcaaggtca ccaggaaagt acatcaccac
     4801 ggccacctca tgattcatct ctttctgctc ccctgcccaa actgtgtgtt tttgttactc
     4861 catctcttct ttcagcaatg tgcagcctct tggacaacct cttgacgatt gctcccagag
     4921 acactgcaaa ggcttttcga caagctcatc tcatagaact tctctgtagc attgcagatg
     4981 ctaccctcat acagacatgt gtccaggaac tcagagccct gctgccttca tcacctccag
     5041 ctgaacacac tcaggctcag gtttcctttc tcctggaata cctatcctct ttgtccaggc
     5101 ttctgcagtc atgtttattg gtggagcctg accttgtgat tcaggatgag cttgtgaaac
     5161 ctcttatcac caatatcatt ggaattctca ccatatgtac caaagatgta ttagataaag
     5221 agttaatatc agctttttat cacacatgga cacatttatt taatcttctg gccatgctcc
     5281 tgaggaaagc tggtgccatc acactcccgt ttgttaccgt ggccctggcc aagcactgga
     5341 cagcggcgat tgatatgttc tgcacatgtg caggcttgtc tgccacgtgt cctgccctgt
     5401 atactgccag cttgcaattc ctttctgttc tcttgaccga agaagcaaaa gggcatctcc
     5461 aggctaagag caaaacacat ttatgctgta gtccaacagt ggcttcactt cttgatgact
     5521 ctcaggaaaa tcagaaatct ctagaacaac ttagtgatgt aatccttcag tgctatgaag
     5581 ggaaatcctc caaagatatc ctgaaaagag tagctgcaaa tgcattgatg tcactgctgg
     5641 ctgtcagtag aagagcacag aaacatgctt tgaaagccaa tcttatagac aattgcatgg
     5701 agcagatgaa acacataaat gcacaactga acctagattc tctgaggcct gggaaagcag
     5761 cattgaaaaa aaaggaggat ggtgttatta aagagttaag cattgccatg cagctcctaa
     5821 gaaactgtct ttatcaaaat gaggaatgta aagaagcagc tcttgaagct caccttgtcc
     5881 ctgtcttgca ctctctctgg ccttggattt tgatggatga ttcattgatg caaatttctc
     5941 tgcagctcct ttgtgtctat actgcaaatt ttccaaatgg ttgcagttct ctttgttggt
     6001 caagttgtgg acaacaccct gttcaagcta cacatagagg agccgtgagc aactctctga
     6061 tgctgtgtat cctaaagttg gcttcccaga tgccactgga gaacaccacg gttcagcaga
     6121 tggtttttat gcttctttca aacctggcct tgtcgcatga ctgtaaagga gtaattcaga
     6181 agagtaactt cttacagaac ttcctctctc tagcattgcc aaaaggagga aataaacatc
     6241 taagtaatct gactattctt tggttgaagt tactcctgaa tatatcatct ggagaagatg
     6301 ggcaacaaat gattctgagg cttgatggct gtctagactt actaacagag atgagcaaat
     6361 acaagcacaa gagcagccct ttattgcctc ttcttatctt tcataatgtt tgcttcagtc
     6421 ctgcaaataa acccaagatc ctggctaatg aaaaagtcat tactgtgctt gctgcctgtc
     6481 tggaaagtga gaatcaaaat gctcagagga ttggagcagc tgccctttgg gctctgattt
     6541 acaattatca gaaggcaaaa acagctttga aaagcccatc agtaaaaaga agagtggatg
     6601 aagcatactc cttagcaaag aaaactttcc caaactcaga agcaaaccct ctaaatgcct
     6661 attatttgaa atgtcttgaa aacctcgtgc agctccttaa ttcttcctga gtgccatggg
     6721 atgctacacc ttgaagctga cagtcatcaa caggggagct aaagttgaag ccagctgtgt
     6781 gtagcagctg ttacctgaag acgtgctacc tctctacaaa gtgttgatcc ccttctttcc
     6841 catgagagag agaactggtg atactccaac accgtccagt tgtggcagct ctccagaagt
     6901 aatagcagct gacaactttc tgtgcctttt cctttctgtt gaaaaggcat agaaagttct
     6961 gggaacataa acatttttac ccttttctat gccatttatt ttgtaaaaat cctatttaac
     7021 agttatttaa taaaacaata tttttagaaa ctaaaaattg actaataatt gctgcttaca
     7081 gacttttatc agtctcatga atattaattt taggagttga aataagacac tgtgtcctgg
     7141 ttgtttctct ctttttttaa attttaactg atgtaaaatt tggctctgtc agtgcaaatc
     7201 cttattttca aaactttctg ttatccagag catccacact ggaaaagggc tatgtagaag
     7261 taagtgtgca ttatttgtaa agggctgtgt ggaagtaaat gtacatgatt tgtaagtagc
     7321 tttcttaggc aagttttagt gatgttatct tcttcctaga tgactcttaa atcattacat
     7381 gtataatctt caccgttgtc ttctgcaagg ctgtgtaata aagaagaaag aaatagagtt
     7441 ggggctgtat aagcctgggt tcaaatttag ctcagcccaa aacatcatga agaatagagt
     7501 aagactgaaa aatgcagaag atggccgggc ttggtggctc atgcttatac tttcagctct
     7561 ttgggagacc gaggtaggtg gatcacttga ggccaggagt ttgagaccag cttgggcaac
     7621 ttggtgaaat cccatctctc ctaaaaatac aaaaattagc cattcctggt cttgagcgtc
     7681 tataatctca gctactccag tggctgaggc acgagaatct cttcaacctg ggaggcaggg
     7741 aggttgcagt gagctgagat catgccactg cattccagcg tgggtgacaa agcaagactc
     7801 tgtctcaaaa aaaaaaaaaa aaaaaaaaaa
//
