LOCUS       NM_001318520            7647 bp    mRNA    linear   PRI 06-APR-2024
DEFINITION  Homo sapiens rotatin (RTTN), transcript variant 2, mRNA.
ACCESSION   NM_001318520 XM_005266679
VERSION     NM_001318520.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7647)
  AUTHORS   Chou,E.J. and Tang,T.K.
  TITLE     Human Microcephaly Protein RTTN Is Required for Proper Mitotic
            Progression and Correct Spindle Position
  JOURNAL   Cells 10 (6), 1441 (2021)
   PUBMED   34207628
  REMARK    GeneRIF: Human Microcephaly Protein RTTN Is Required for Proper
            Mitotic Progression and Correct Spindle Position.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7647)
  AUTHORS   Zakaria,M., Fatima,A., Klar,J., Wikstrom,J., Abdullah,U., Ali,Z.,
            Akram,T., Tariq,M., Ahmad,H., Schuster,J., Baig,S.M. and Dahl,N.
  TITLE     Primary microcephaly, primordial dwarfism, and brachydactyly in
            adult cases with biallelic skipping of RTTN exon 42
  JOURNAL   Hum Mutat 40 (7), 899-903 (2019)
   PUBMED   30927481
  REMARK    GeneRIF: Study reports on a consanguineous family with three adult
            members with primary microcephaly, developmental delay, primordial
            dwarfism, and brachydactyly segregating a homozygous splice site
            variant NM_173630.3:c.5648-5T>A in RTTN. The variant RTTN allele
            results in a nonhypomorphic skipping of exon 42 and a frameshift
            [(NP_775901.3:p.Ala1883Glyfs*6)].
REFERENCE   3  (bases 1 to 7647)
  AUTHORS   Cavallin,M., Bery,A., Maillard,C., Salomon,L.J., Bole,C.,
            Reilly,M.L., Nitschke,P., Boddaert,N. and Bahi-Buisson,N.
  TITLE     Recurrent RTTN mutation leading to severe microcephaly,
            polymicrogyria and growth restriction
  JOURNAL   Eur J Med Genet 61 (12), 755-758 (2018)
   PUBMED   30121372
  REMARK    GeneRIF: We identified, by trio based whole exome sequencing, a
            homozygous missense mutation in the RTTN gene (c.2953A>G;
            p.(Arg985Gly)) in one Moroccan patient from a consanguineous
            family.
REFERENCE   4  (bases 1 to 7647)
  AUTHORS   Stouffs,K., Moortgat,S., Vanderhasselt,T., Vandervore,L., Dica,A.,
            Mathot,M., Keymolen,K., Seneca,S., Gheldof,A., De Meirleir,L. and
            Jansen,A.C.
  TITLE     Biallelic mutations in RTTN are associated with microcephaly, short
            stature and a wide range of brain malformations
  JOURNAL   Eur J Med Genet 61 (12), 733-737 (2018)
   PUBMED   29883675
  REMARK    GeneRIF: We here report three individuals from two unrelated
            families with novel mutations in the RTTN gene. The phenotype
            consisted of microcephaly, short stature, pachygyria or
            polymicrogyria, colpocephaly, hypoplasia of the corpus callosum and
            superior vermis. These findings provide further confirmation of the
            phenotype related to pathogenic variants in RTTN.
REFERENCE   5  (bases 1 to 7647)
  AUTHORS   Sydor,A.M., Coyaud,E., Rovelli,C., Laurent,E., Liu,H., Raught,B.
            and Mennella,V.
  TITLE     PPP1R35 is a novel centrosomal protein that regulates centriole
            length in concert with the microcephaly protein RTTN
  JOURNAL   Elife 7, e37846 (2018)
   PUBMED   30168418
  REMARK    GeneRIF: Data show that PPP1R35 acts downstream of, and forms a
            complex with, RTTN, a microcephaly protein required for distal
            centriole elongation.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 7647)
  AUTHORS   Shamseldin,H., Alazami,A.M., Manning,M., Hashem,A., Caluseiu,O.,
            Tabarki,B., Esplin,E., Schelley,S., Innes,A.M., Parboosingh,J.S.,
            Lamont,R., Majewski,J., Bernier,F.P. and Alkuraya,F.S.
  CONSRTM   Care4Rare Canada Consortium
  TITLE     RTTN Mutations Cause Primary Microcephaly and Primordial Dwarfism
            in Humans
  JOURNAL   Am J Hum Genet 97 (6), 862-868 (2015)
   PUBMED   26608784
  REMARK    GeneRIF: RTTN mutations cause primary microcephaly and primordial
            dwarfism in humans.
REFERENCE   7  (bases 1 to 7647)
  AUTHORS   Toro,C., Olive,M., Dalakas,M.C., Sivakumar,K., Bilbao,J.M.,
            Tyndel,F., Vidal,N., Farrero,E., Sambuughin,N. and Goldfarb,L.G.
  TITLE     Exome sequencing identifies titin mutations causing hereditary
            myopathy with early respiratory failure (HMERF) in families of
            diverse ethnic origins
  JOURNAL   BMC Neurol 13, 29 (2013)
   PUBMED   23514108
  REMARK    Publication Status: Online-Only
REFERENCE   8  (bases 1 to 7647)
  AUTHORS   Kheradmand Kia,S., Verbeek,E., Engelen,E., Schot,R., Poot,R.A., de
            Coo,I.F., Lequin,M.H., Poulton,C.J., Pourfarzad,F., Grosveld,F.G.,
            Brehm,A., de Wit,M.C., Oegema,R., Dobyns,W.B., Verheijen,F.W. and
            Mancini,G.M.
  TITLE     RTTN mutations link primary cilia function to organization of the
            human cerebral cortex
  JOURNAL   Am J Hum Genet 91 (3), 533-540 (2012)
   PUBMED   22939636
  REMARK    GeneRIF: RTTN mutations therefore link aberrant ciliary function to
            abnormal development and organization of the cortex in human
            individuals.
REFERENCE   9  (bases 1 to 7647)
  AUTHORS   Stevens,N.R., Dobbelaere,J., Wainman,A., Gergely,F. and Raff,J.W.
  TITLE     Ana3 is a conserved protein required for the structural integrity
            of centrioles and basal bodies
  JOURNAL   J Cell Biol 187 (3), 355-363 (2009)
   PUBMED   19948479
REFERENCE   10 (bases 1 to 7647)
  AUTHORS   Faisst,A.M., Alvarez-Bolado,G., Treichel,D. and Gruss,P.
  TITLE     Rotatin is a novel gene required for axial rotation and left-right
            specification in mouse embryos
  JOURNAL   Mech Dev 113 (1), 15-28 (2002)
   PUBMED   11900971
  REMARK    GeneRIF: Study characterizing mouse rotatin gene.
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK096404.1, AC021701.8,
            BC143723.1, BC046222.2, AL117635.1 and AC011930.5.
            
            On May 31, 2019 this sequence version replaced NM_001318520.1.
            
            Summary: This gene encodes a large protein whose specific function
            is unknown. Absence of the orthologous protein in mouse results in
            embryonic lethality with deficient axial rotation, abnormal
            differentiation of the neural tube, and randomized looping of the
            heart tube during development. In human, mutations in this gene are
            associated with polymicrogyria with seizures. In human fibroblasts
            this protein localizes at the ciliary basal bodies. Given the
            intracellular localization of this protein and the phenotypic
            effects of mutations, this gene is suspected of playing a role in
            the maintenance of normal ciliary structure which in turn effects
            the developmental process of left-right organ specification, axial
            rotation, and perhaps notochord development. [provided by RefSeq,
            Jan 2013].
            
            Transcript Variant: This variant (2) uses an alternate splice site
            and lacks an exon in its 5' UTR, and uses a downstream in-frame
            start codon, compared to variant 1. The encoded isoform (2) has a
            shorter N-terminus than isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC143723.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-404               AK096404.1         43-446
            405-405             AC021701.8         129482-129482       c
            406-756             AK096404.1         448-798
            757-5127            BC143723.1         773-5143
            5128-5646           BC046222.2         548-1066
            5647-6709           BC143723.1         5663-6725
            6710-6874           AL117635.1         768-932
            6875-7647           AC011930.5         102343-103115
FEATURES             Location/Qualifiers
     source          1..7647
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="18"
                     /map="18q22.2"
     gene            1..7647
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /note="rotatin"
                     /db_xref="GeneID:25914"
                     /db_xref="HGNC:HGNC:18654"
                     /db_xref="MIM:610436"
     exon            1..60
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            61..248
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            249..426
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            427..516
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            517..607
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            608..722
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            723..870
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            871..1036
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            1037..1218
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            1219..1334
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            1335..1505
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            1506..1624
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            1625..1737
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            1738..1864
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            1865..1990
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            1991..2107
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            2108..2244
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            2245..2416
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            2417..2516
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            2517..2634
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     CDS             2583..6527
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /note="isoform 2 is encoded by transcript variant 2"
                     /codon_start=1
                     /product="rotatin isoform 2"
                     /protein_id="NP_001305449.1"
                     /db_xref="GeneID:25914"
                     /db_xref="HGNC:HGNC:18654"
                     /db_xref="MIM:610436"
                     /translation="MRVSLSQQSSLLTVLFRVSLIFHEDCSVVTEVGALFCLLLFDEV
                     SRMDMWSVNPSNKPSLPSVFSLPVSVFRRYHLPVHVIGHHAVSPYSIVLPLSADCLAL
                     KPVSDMLRIAWNLSWYHGSDNLLKQMNSETKTQEILDALKLSTEDILTLKITHMASGL
                     QDCLHSIVQAATHREVRAAVTRMSFYLLNDRLSLKGCPGPCGVTLKSLAWHTALNRFL
                     QVLPACTEDEKLLIDIIHFLNKLIKEQRKNSSLELLNWILELLLRHSANPLLDLLVLT
                     ESQAREETDDIRTAVRQQLQKELIALFDTLLLNFMEVTDRKCSELLYVFQTQLALKLL
                     QCLKVTDAPHFYGLPSLERTLRGMANLTAFPGWSSHSPLTKPLDICVKYLSGLLEVIT
                     SFYVERGGNAMSFMGKGVTKSTILCLLHLSHEMMAQAGSLEWMSLWFLPLGSHSEEHI
                     PTQQGLAWLIPLWVDRDPEVRFTSLGLGSALTTLETGCVALANSCQNISGGLWGTVVN
                     ILLDQSECSMVRREAAFILQNLLVIPMPTEIIKDYTWQGPCVHDEDSGLSLIGKPALQ
                     ALLYHCHFYEHLNQMVKHCYLGRCMFDLNFSAFDRNSESNDLNGLDDSFKFWRAPSRT
                     SQDRDPSSLSTSETTVAPSLGSTEFQPLVQSTTLLPEASHDQFVAQGHQESTSPRPPH
                     DSSLSAPLPKLCVFVTPSLLSAMCSLLDNLLTIAPRDTAKAFRQAHLIELLCSIADAT
                     LIQTCVQELRALLPSSPPAEHTQAQVSFLLEYLSSLSRLLQSCLLVEPDLVIQDELVK
                     PLITNIIGILTICTKDVLDKELISAFYHTWTHLFNLLAMLLRKAGAITLPFVTVALAK
                     HWTAAIDMFCTCAGLSATCPALYTASLQFLSVLLTEEAKGHLQAKSKTHLCCSPTVAS
                     LLDDSQENQKSLEQLSDVILQCYEGKSSKDILKRVAANALMSLLAVSRRAQKHALKAN
                     LIDNCMEQMKHINAQLNLDSLRPGKAALKKKEDGVIKELSIAMQLLRNCLYQNEECKE
                     AALEAHLVPVLHSLWPWILMDDSLMQISLQLLCVYTANFPNGCSSLCWSSCGQHPVQA
                     THRGAVSNSLMLCILKLASQMPLENTTVQQMVFMLLSNLALSHDCKGVIQKSNFLQNF
                     LSLALPKGGNKHLSNLTILWLKLLLNISSGEDGQQMILRLDGCLDLLTEMSKYKHKSS
                     PLLPLLIFHNVCFSPANKPKILANEKVITVLAACLESENQNAQRIGAAALWALIYNYQ
                     KAKTALKSPSVKRRVDEAYSLAKKTFPNSEANPLNAYYLKCLENLVQLLNSS"
     exon            2635..2731
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            2732..2800
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            2801..2989
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            2990..3229
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            3230..3374
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            3375..3529
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            3530..3749
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            3750..3878
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            3879..3989
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            3990..4148
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            4149..4220
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            4221..4410
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            4411..4499
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            4500..4593
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            4594..4786
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            4787..4877
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            4878..5031
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            5032..5169
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            5170..5387
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            5388..5493
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            5494..5591
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            5592..5669
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            5670..5796
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            5797..5999
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            6000..6267
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            6268..6371
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            6372..6441
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     exon            6442..7647
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6846..6851
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /note="hexamer: AATAAA"
     polyA_site      6874
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /note="major polyA site"
     regulatory      7234..7239
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RTTN"
                     /gene_synonym="MSSP"
                     /note="hexamer: AATAAA"
     polyA_site      7254
                     /gene="RTTN"
                     /gene_synonym="MSSP"
ORIGIN      
        1 ctccggcggc tgcagattga cgggacgaga tggtcctggc agggctcatc aggaaactcg
       61 gtcatcagct ggccgagatc agggagcgcg ctctcaagag tattctctgc aagattgagc
      121 acaacttaat ctgctacgct gatctcattc aggagaggca actttttctt catttgctgg
      181 aatggttcaa tttcccgtcc gttccgatga aggaagaggt tctgaacctg ttaagcagat
      241 tggttaagta tcccccagca gtccaacatt tggttgacgt tggtgcagta gagttcttat
      301 ctaagcttcg gtctaatgtg gagccaaatc tgcaggctga aattgatggc attctggatg
      361 gactttttct tcttccttcg gaagttcctg cactatcttc tgcctcatac caaaccaatc
      421 aaactgaatt gtcaaaaaac cctgaaatct taacaggata ttttccccaa gacaaaagta
      481 atttccagca gatggaagtg ccgccacgac cagtggtaaa tcagactgtg aagtgcttga
      541 agttttctac atttccttgg ctacccctga ccaccacaga cagacatgtc ctctcctcta
      601 atgaaagctc tttaagaagt agtaaccaca ctttaatctg gaacacctgt gaactattga
      661 aggatgttat catgcaagat tttcctgctg agattttcct tcaaaggcca aaaattgttc
      721 agagcctttt atctctgttg aaactggcct ttggagatgg aaagcatcgc ctggcattac
      781 agtcggtgtc ctgcctgcag cagctgtgca tgtatttaag aaacagactt aactttcacc
      841 gagatccagg ttttttctcc aataaacacg acacagtttc ccaaaattct tctttgtctt
      901 attgtcatga agcaagaggt actcatcatt cccagaatcc ttccccagga agcagcagcc
      961 ctcggccttc tgtggttggg cgcacaggcc agcgacccag aggagatggc caggactggg
     1021 atgcagcgtc ctctagtgga agtagtagtc atgctcatgt aaactccagg atatccgttc
     1081 attcaccttt ggatatggga cacatagatc tgccagagct ggaaactgaa gacacattgg
     1141 aactacaatt ccagcagctc agtcttcccc agttttgtgt ctccattctg gaatcagctg
     1201 ttcctctctt aagaacaggt agcagacaag tgataataag agttctggaa ttgcttacag
     1261 aggatatgac acttattggt gaagcaattt caacagatat ctgggatgac agcagccttt
     1321 ttggtataga tatgaaggag aaactactcc tggtgttggg agcccttgga gaaaccatgt
     1381 gctaccataa aagtagtatc agtttggagc agccagaggt gatgcttgtg caccacagaa
     1441 tggcctttat cagcatttcc ctgtttgcag ttcgactgct acaaacgctt ctccctgttg
     1501 aaaagctgtt gtggcctatt tggaacagct gaattctgaa aactacagta tttataaacg
     1561 aactgcagag gccgtttatt caattgaatg cacctgtaac ttcctgtctg atattgggaa
     1621 agagggagag aagaatctct tagaattagt ggagctggca gaccaagcct tgcgtagctt
     1681 ttcctatcat cagcatttcc cgctaataaa ggaaatcatc agcatttgtt caaagatctg
     1741 gaaatctgct caggccagtc cattactaca aggagaaagt cagaaggtgc ttctccatat
     1801 gttgtctcac ccattgccac gagtgaaagc tgaaacttac cactgctgtc tggaaatcac
     1861 gaaggaatgt ttaggtgtcc ataatgtcac taaacccgtg tcttcacttt gcaatggaat
     1921 tcattttcta cttcatccaa aagttctgta tgaaatctct gtatttggca ttcaagagcc
     1981 cgaaagtgag gtgaacactg ctgccaaggc cattctgtta tacctgcttc agggacgatt
     2041 gatgatgaca gcattgacct ggaacaagtt tattgaatct ctctgtcctg tcatcccaat
     2101 actacagggc tatgccgaca cagaagatcc tctgggtaac tgcattctcc ttctaagcaa
     2161 agcaagttct gacacagaag agatgcttcc gtgtactacc cgattaaagt ccatgctgcg
     2221 acttttgcta gtgaaaaagc catcggtccg ttcgctagca ttaaagcttt tagcattcca
     2281 tcttaccagt gaagaagggg ctgatacaaa gcgtcctcta atagacgcca gagttctctc
     2341 cagagttact gatttattca ttgggaagaa acctattgaa ctcagactgg atgacagaag
     2401 agagctggtt attaagttgg agactgttga aaaggtatat gaaatcttca cctcagatga
     2461 tgttgatctc gttttgagaa agtcagctgc tgaacagtta gctgtgatta tgcaaggttg
     2521 tagaatgttt ggtacaacca tgcctcacac tcttgaggaa ggttttatgt ggtgatccag
     2581 tcatgcgtgt ttcgctctcg caacagtctt ctcttttgac cgtgttattc agagtttcct
     2641 taatatttca tgaagattgt agtgtcgtga ctgaagttgg agcactattt tgtcttctat
     2701 tatttgatga agtatcgaga atggatatgt ggtctgttaa tccttccaat aaaccttctt
     2761 tgccatcggt cttcagtttg cctgtttccg tttttagaag gtaccatcta cctgttcatg
     2821 taattggaca ccatgctgtg agtccttact ccatagtttt gcccttatct gctgattgtt
     2881 tggccttgaa gccggtgtca gatatgctga gaatagcttg gaacctgtca tggtatcatg
     2941 ggagtgataa tctgttgaag caaatgaact ctgaaactaa aacgcaagaa attttagatg
     3001 cattgaagtt atctacagag gacatcctca ctctgaagat aacacacatg gctagtgggc
     3061 tgcaggactg cctccattcc attgttcagg ctgcaaccca cagggaagtt agggctgctg
     3121 tcaccaggat gagtttttat cttctgaatg acagattgtc tttaaagggt tgccctggtc
     3181 catgtggggt taccttgaag tccttggctt ggcacaccgc actaaacagg tttttacagg
     3241 tgcttcctgc ttgcactgaa gatgagaaac tgctaataga tatcatacat tttttaaata
     3301 aattaataaa ggaacaaaga aaaaattctt cactagaact tctaaactgg attctcgaat
     3361 tacttcttag acatagtgca aacccactgt tagacctctt ggttctgaca gagtcacagg
     3421 cacgagaaga aacagatgat atccggactg ctgtcaggca acaacttcag aaagaactga
     3481 ttgctctttt tgataccttg ctgctcaatt tcatggaagt tactgacagg aaatgctcgg
     3541 aacttcttta cgtttttcaa acgcagctgg ctctgaaact gctccagtgt ctgaaagtga
     3601 cggatgcgcc tcatttctat ggcctgccgt cccttgagcg gaccttacga gggatggcta
     3661 acctcactgc gtttccggga tggagctcac actctcctct cacaaagcct ctagatatct
     3721 gtgtgaagta cttgtcaggt ctccttgagg tcattacttc tttttatgtg gagcgtggag
     3781 gaaatgctat gtccttcatg ggaaaaggtg ttacaaagag cacaattctt tgcttgcttc
     3841 acttatccca tgagatgatg gcccaggctg ggagcttgga gtggatgtca ctttggttct
     3901 tgcctttggg tagtcatagt gaagaacata ttcctactca acaaggattg gcttggttga
     3961 ttccattatg ggttgatcgg gacccagagg tgagattcac ttcactggga ttaggatcag
     4021 cactgaccac ccttgaaacg ggctgtgtgg ccttagcaaa cagttgtcag aacatttccg
     4081 gtgggctctg gggaactgtg gtgaacattc ttctggacca gtcagaatgt agtatggtgc
     4141 gccgggaggc ggcatttatt cttcagaatc tccttgtaat tccaatgcct acagaaatta
     4201 taaaggatta tacttggcag ggtccctgtg ttcatgatga ggactctggc ctatcgctca
     4261 ttggaaaacc tgcccttcag gctcttttat atcactgcca tttttatgaa catttgaatc
     4321 agatggtaaa gcattgttac ctaggacggt gtatgtttga tttgaatttt tctgcttttg
     4381 atagaaattc agaaagcaat gatttaaatg gtttagatga ctcattcaag ttttggaggg
     4441 ctccatctag gacaagtcag gatcgagatc caagttctct ctccacctca gaaacaacgg
     4501 tggcaccttc attggggagt actgaatttc agccacttgt gcagtcaaca acacttctac
     4561 ctgaagcctc ccatgaccag tttgtggctc aaggtcacca ggaaagtaca tcaccacggc
     4621 cacctcatga ttcatctctt tctgctcccc tgcccaaact gtgtgttttt gttactccat
     4681 ctcttctttc agcaatgtgc agcctcttgg acaacctctt gacgattgct cccagagaca
     4741 ctgcaaaggc ttttcgacaa gctcatctca tagaacttct ctgtagcatt gcagatgcta
     4801 ccctcataca gacatgtgtc caggaactca gagccctgct gccttcatca cctccagctg
     4861 aacacactca ggctcaggtt tcctttctcc tggaatacct atcctctttg tccaggcttc
     4921 tgcagtcatg tttattggtg gagcctgacc ttgtgattca ggatgagctt gtgaaacctc
     4981 ttatcaccaa tatcattgga attctcacca tatgtaccaa agatgtatta gataaagagt
     5041 taatatcagc tttttatcac acatggacac atttatttaa tcttctggcc atgctcctga
     5101 ggaaagctgg tgccatcaca ctcccgtttg ttaccgtggc cctggccaag cactggacag
     5161 cggcgattga tatgttctgc acatgtgcag gcttgtctgc cacgtgtcct gccctgtata
     5221 ctgccagctt gcaattcctt tctgttctct tgaccgaaga agcaaaaggg catctccagg
     5281 ctaagagcaa aacacattta tgctgtagtc caacagtggc ttcacttctt gatgactctc
     5341 aggaaaatca gaaatctcta gaacaactta gtgatgtaat ccttcagtgc tatgaaggga
     5401 aatcctccaa agatatcctg aaaagagtag ctgcaaatgc attgatgtca ctgctggctg
     5461 tcagtagaag agcacagaaa catgctttga aagccaatct tatagacaat tgcatggagc
     5521 agatgaaaca cataaatgca caactgaacc tagattctct gaggcctggg aaagcagcat
     5581 tgaaaaaaaa ggaggatggt gttattaaag agttaagcat tgccatgcag ctcctaagaa
     5641 actgtcttta tcaaaatgag gaatgtaaag aagcagctct tgaagctcac cttgtccctg
     5701 tcttgcactc tctctggcct tggattttga tggatgattc attgatgcaa atttctctgc
     5761 agctcctttg tgtctatact gcaaattttc caaatggttg cagttctctt tgttggtcaa
     5821 gttgtggaca acaccctgtt caagctacac atagaggagc cgtgagcaac tctctgatgc
     5881 tgtgtatcct aaagttggct tcccagatgc cactggagaa caccacggtt cagcagatgg
     5941 tttttatgct tctttcaaac ctggccttgt cgcatgactg taaaggagta attcagaaga
     6001 gtaacttctt acagaacttc ctctctctag cattgccaaa aggaggaaat aaacatctaa
     6061 gtaatctgac tattctttgg ttgaagttac tcctgaatat atcatctgga gaagatgggc
     6121 aacaaatgat tctgaggctt gatggctgtc tagacttact aacagagatg agcaaataca
     6181 agcacaagag cagcccttta ttgcctcttc ttatctttca taatgtttgc ttcagtcctg
     6241 caaataaacc caagatcctg gctaatgaaa aagtcattac tgtgcttgct gcctgtctgg
     6301 aaagtgagaa tcaaaatgct cagaggattg gagcagctgc cctttgggct ctgatttaca
     6361 attatcagaa ggcaaaaaca gctttgaaaa gcccatcagt aaaaagaaga gtggatgaag
     6421 catactcctt agcaaagaaa actttcccaa actcagaagc aaaccctcta aatgcctatt
     6481 atttgaaatg tcttgaaaac ctcgtgcagc tccttaattc ttcctgagtg ccatgggatg
     6541 ctacaccttg aagctgacag tcatcaacag gggagctaaa gttgaagcca gctgtgtgta
     6601 gcagctgtta cctgaagacg tgctacctct ctacaaagtg ttgatcccct tctttcccat
     6661 gagagagaga actggtgata ctccaacacc gtccagttgt ggcagctctc cagaagtaat
     6721 agcagctgac aactttctgt gccttttcct ttctgttgaa aaggcataga aagttctggg
     6781 aacataaaca tttttaccct tttctatgcc atttattttg taaaaatcct atttaacagt
     6841 tatttaataa aacaatattt ttagaaacta aaaattgact aataattgct gcttacagac
     6901 ttttatcagt ctcatgaata ttaattttag gagttgaaat aagacactgt gtcctggttg
     6961 tttctctctt tttttaaatt ttaactgatg taaaatttgg ctctgtcagt gcaaatcctt
     7021 attttcaaaa ctttctgtta tccagagcat ccacactgga aaagggctat gtagaagtaa
     7081 gtgtgcatta tttgtaaagg gctgtgtgga agtaaatgta catgatttgt aagtagcttt
     7141 cttaggcaag ttttagtgat gttatcttct tcctagatga ctcttaaatc attacatgta
     7201 taatcttcac cgttgtcttc tgcaaggctg tgtaataaag aagaaagaaa tagagttggg
     7261 gctgtataag cctgggttca aatttagctc agcccaaaac atcatgaaga atagagtaag
     7321 actgaaaaat gcagaagatg gccgggcttg gtggctcatg cttatacttt cagctctttg
     7381 ggagaccgag gtaggtggat cacttgaggc caggagtttg agaccagctt gggcaacttg
     7441 gtgaaatccc atctctccta aaaatacaaa aattagccat tcctggtctt gagcgtctat
     7501 aatctcagct actccagtgg ctgaggcacg agaatctctt caacctggga ggcagggagg
     7561 ttgcagtgag ctgagatcat gccactgcat tccagcgtgg gtgacaaagc aagactctgt
     7621 ctcaaaaaaa aaaaaaaaaa aaaaaaa
//
