LOCUS       NM_005619               2274 bp    mRNA    linear   PRI 08-OCT-2024
DEFINITION  Homo sapiens reticulon 2 (RTN2), transcript variant 1, mRNA.
ACCESSION   NM_005619
VERSION     NM_005619.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Wang,G., Xu,Z., Sun,J., Liu,B., Ruan,Y., Gu,J. and Song,S.
  TITLE     O-GlcNAcylation enhances Reticulon 2 protein stability and its
            promotive effects on gastric cancer progression
  JOURNAL   Cell Signal 108, 110718 (2023)
   PUBMED   37196774
  REMARK    GeneRIF: O-GlcNAcylation enhances Reticulon 2 protein stability and
            its promotive effects on gastric cancer progression.
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Zheng,X., Lv,X., Chai,J., Huang,Y., Zhu,L. and Zhang,X.
  TITLE     RTN2, a new member of circadian clock genes identified by database
            mining and bioinformatics prediction, is highly expressed in
            ovarian cancer
  JOURNAL   Mol Med Rep 26 (5) (2022)
   PUBMED   36177918
  REMARK    GeneRIF: RTN2, a new member of circadian clock genes identified by
            database mining and bioinformatics prediction, is highly expressed
            in ovarian cancer.
REFERENCE   3  (bases 1 to 2274)
  AUTHORS   Tian,W., Zheng,H., Zhu,Z., Zhang,C., Luan,X. and Cao,L.
  TITLE     New phenotype of RTN2-related spectrum: Complicated form of spastic
            paraplegia-12
  JOURNAL   Ann Clin Transl Neurol 9 (8), 1108-1115 (2022)
   PUBMED   35684947
  REMARK    GeneRIF: New phenotype of RTN2-related spectrum: Complicated form
            of spastic paraplegia-12.
REFERENCE   4  (bases 1 to 2274)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   5  (bases 1 to 2274)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   6  (bases 1 to 2274)
  AUTHORS   Reid,E., Dearlove,A.M., Osborn,O., Rogers,M.T. and Rubinsztein,D.C.
  TITLE     A locus for autosomal dominant 'pure' hereditary spastic paraplegia
            maps to chromosome 19q13
  JOURNAL   Am J Hum Genet 66 (2), 728-732 (2000)
   PUBMED   10677333
REFERENCE   7  (bases 1 to 2274)
  AUTHORS   Geisler,J.G., Palmer,R.J., Stubbs,L.J. and Mucenski,M.L.
  TITLE     Nspl1, a new Z-band-associated protein
  JOURNAL   J Muscle Res Cell Motil 20 (7), 661-668 (1999)
   PUBMED   10672514
REFERENCE   8  (bases 1 to 2274)
  AUTHORS   Roebroek,A.J., Contreras,B., Pauli,I.G. and Van de Ven,W.J.
  TITLE     cDNA cloning, genomic organization, and expression of the human
            RTN2 gene, a member of a gene family encoding reticulons
  JOURNAL   Genomics 51 (1), 98-106 (1998)
   PUBMED   9693037
REFERENCE   9  (bases 1 to 2274)
  AUTHORS   Geisler,J.G., Stubbs,L.J., Wasserman,W.W. and Mucenski,M.L.
  TITLE     Molecular cloning of a novel mouse gene with predominant muscle and
            neural expression
  JOURNAL   Mamm Genome 9 (4), 274-282 (1998)
   PUBMED   9530622
  REMARK    GeneRIF: Discusses cloning of mouse neuroendocrine-specific
            protein-like 1, in addition to partial cloning of the human
            ortholog. Also studies expression of both the mouse and human
            genes.
REFERENCE   10 (bases 1 to 2274)
  AUTHORS   Hedera,P.
  TITLE     Hereditary Spastic Paraplegia Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301682
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BK001687.1, BC073874.1 and
            AF004222.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_005619.4.
            
            Summary: This gene belongs to the family of reticulon encoding
            genes. Reticulons are associated with the endoplasmic reticulum,
            and are involved in neuroendocrine secretion or in membrane
            trafficking in neuroendocrine cells. Reticulon proteins also play
            an important role in the replication of positive-strand RNA (ssRNA)
            viruses. Mutations at this locus have been associated with
            autosomal dominant spastic paraplegia-12. [provided by RefSeq, Aug
            2020].
            
            Transcript Variant: This variant (1) represents the longest
            transcript, and encodes the longest isoform (A).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BK001687.1, BC073874.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000245923.9/ ENSP00000245923.3
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-92                BK001687.1         9-100
            93-93               BC073874.1         43-43
            94-2274             AF004222.1         1-2181
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19q13.32"
     gene            1..2274
                     /gene="RTN2"
                     /gene_synonym="HMNR11; NSP2; NSPL1; NSPLI; SPG12"
                     /note="reticulon 2"
                     /db_xref="GeneID:6253"
                     /db_xref="HGNC:HGNC:10468"
                     /db_xref="MIM:603183"
     exon            1..256
                     /gene="RTN2"
                     /gene_synonym="HMNR11; NSP2; NSPL1; NSPLI; SPG12"
                     /inference="alignment:Splign:2.1.0"
     CDS             223..1860
                     /gene="RTN2"
                     /gene_synonym="HMNR11; NSP2; NSPL1; NSPLI; SPG12"
                     /note="isoform A is encoded by transcript variant 1;
                     NSP-like protein 1; NSP-like protein I;
                     neuroendocrine-specific protein-like I"
                     /codon_start=1
                     /product="reticulon-2 isoform A"
                     /protein_id="NP_005610.1"
                     /db_xref="CCDS:CCDS12665.1"
                     /db_xref="GeneID:6253"
                     /db_xref="HGNC:HGNC:10468"
                     /db_xref="MIM:603183"
                     /translation="MGQVLPVFAHCKEAPSTASSTPDSTEGGNDDSDFRELHTAREFS
                     EEDEEETTSQDWGTPRELTFSYIAFDGVVGSGGRRDSTARRPRPQGRSVSEPRDQHPQ
                     PSLGDSLESIPSLSQSPEPGRRGDPDTAPPSERPLEDLRLRLDHLGWVARGTGSGEDS
                     STSSSTPLEDEEPQEPNRLETGEAGEELDLRLRLAQPSSPEVLTPQLSPGSGTPQAGT
                     PSPSRSRDSNSGPEEPLLEEEEKQWGPLEREPVRGQCLDSTDQLEFTVEPRLLGTAME
                     WLKTSLLLAVYKTVPILELSPPLWTAIGWVQRGPTPPTPVLRVLLKWAKSPRSSGVPS
                     LSLGADMGSKVADLLYWKDTRTSGVVFTGLMVSLLCLLHFSIVSVAAHLALLLLCGTI
                     SLRVYRKVLQAVHRGDGANPFQAYLDVDLTLTREQTERLSHQITSRVVSAATQLRHFF
                     LVEDLVDSLKLALLFYILTFVGAIFNGLTLLILGVIGLFTIPLLYRQHQAQIDQYVGL
                     VTNQLSHIKAKIRAKIPGTGALASAAAAVSGSKAKAE"
     exon            257..301
                     /gene="RTN2"
                     /gene_synonym="HMNR11; NSP2; NSPL1; NSPLI; SPG12"
                     /inference="alignment:Splign:2.1.0"
     exon            302..781
                     /gene="RTN2"
                     /gene_synonym="HMNR11; NSP2; NSPL1; NSPLI; SPG12"
                     /inference="alignment:Splign:2.1.0"
     exon            782..1036
                     /gene="RTN2"
                     /gene_synonym="HMNR11; NSP2; NSPL1; NSPLI; SPG12"
                     /inference="alignment:Splign:2.1.0"
     exon            1037..1255
                     /gene="RTN2"
                     /gene_synonym="HMNR11; NSP2; NSPL1; NSPLI; SPG12"
                     /inference="alignment:Splign:2.1.0"
     exon            1256..1463
                     /gene="RTN2"
                     /gene_synonym="HMNR11; NSP2; NSPL1; NSPLI; SPG12"
                     /inference="alignment:Splign:2.1.0"
     exon            1464..1602
                     /gene="RTN2"
                     /gene_synonym="HMNR11; NSP2; NSPL1; NSPLI; SPG12"
                     /inference="alignment:Splign:2.1.0"
     exon            1603..1672
                     /gene="RTN2"
                     /gene_synonym="HMNR11; NSP2; NSPL1; NSPLI; SPG12"
                     /inference="alignment:Splign:2.1.0"
     exon            1673..1719
                     /gene="RTN2"
                     /gene_synonym="HMNR11; NSP2; NSPL1; NSPLI; SPG12"
                     /inference="alignment:Splign:2.1.0"
     exon            1720..1778
                     /gene="RTN2"
                     /gene_synonym="HMNR11; NSP2; NSPL1; NSPLI; SPG12"
                     /inference="alignment:Splign:2.1.0"
     exon            1779..2274
                     /gene="RTN2"
                     /gene_synonym="HMNR11; NSP2; NSPL1; NSPLI; SPG12"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2254..2259
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RTN2"
                     /gene_synonym="HMNR11; NSP2; NSPL1; NSPLI; SPG12"
                     /note="hexamer: AATAAA"
     polyA_site      2274
                     /gene="RTN2"
                     /gene_synonym="HMNR11; NSP2; NSPL1; NSPLI; SPG12"
                     /note="major polyA site"
ORIGIN      
        1 gcagtgcctt ccccacctcg gccccgcccg cccccgccga gccgagcacc agggcggcgg
       61 cggcggcggc ggcggcggcg gcggctggag cagcccggga ggaggaggcg gcgagaatgg
      121 cagcggcgtc gtgggcgcgg cggagatgag cgcccgcgac cccgggccca gggcggcaca
      181 gccggagtgg gcgggggtcc cgatgcaggc ccgagggggg ccatggggca ggtcctgccg
      241 gtcttcgccc actgcaaaga agctccgtct acagcctcct caactcctga ttccacagaa
      301 ggagggaacg acgactctga ttttcgagag ctgcacacag cccgggaatt ctcagaggag
      361 gacgaggagg agaccacgtc gcaggactgg ggcacccccc gggagctgac cttctcctac
      421 atcgcctttg atggtgtagt gggctccggg ggccgcaggg attcaactgc ccgccgcccc
      481 cgcccccagg gccgctcagt ctcggaacca cgagaccagc accctcagcc cagcctgggc
      541 gacagcttgg agagcatccc cagcctgagc caatccccgg agcctggacg acggggtgat
      601 cctgacaccg cgcctccatc cgagcgccct ctggaagacc tgaggcttcg gttggaccat
      661 ctgggctggg tggcccgggg aacgggatcc ggggaggact cttccaccag cagctccacc
      721 ccgctggaag acgaagaacc ccaagaaccc aacagattgg agacaggaga agctggggaa
      781 gaactggacc tacgactccg acttgctcag ccctcatcgc ccgaggtctt gactccccag
      841 ctcagtccgg gctctgggac accccaggcc ggtactccgt ccccatcccg atcgcgagat
      901 tcgaactctg ggcccgaaga gccattgctg gaagaggaag aaaagcagtg ggggccactg
      961 gagcgagagc cagtaagggg acagtgcctc gatagcacgg accaattaga attcacggtg
     1021 gagccacgcc ttctaggaac agctatggaa tggttaaaga catcattgct tttggctgtt
     1081 tacaagacgg ttccaatttt ggaattgtcc ccacctctgt ggacagccat tggctgggtc
     1141 caaaggggcc ccaccccccc tactcctgtc ctccgggttc tactgaagtg ggcaaaatcc
     1201 ccgagaagca gcggtgtccc cagcctctca ctcggagccg atatggggag taaagtggcg
     1261 gacctgctgt actggaagga cacgaggacg tcaggagtgg tcttcacagg cctgatggtc
     1321 tccctcctct gcctcctgca ctttagcatc gtgtccgtgg ccgcgcactt ggctctgttg
     1381 ctgctctgcg gcaccatctc tctcagggtt taccgcaaag tgctgcaggc cgtgcaccgg
     1441 ggggatggag ccaacccttt ccaggcctac ctggatgtgg acctcaccct gactcgggag
     1501 cagacggaac gtttgtccca ccagatcacc tcccgcgtgg tctcggcggc cacgcagctg
     1561 cggcacttct tcctggtaga agacctcgtg gattccctca agctggccct cctcttctac
     1621 atcttgacct tcgtgggtgc catcttcaat ggtttgactc ttctcattct gggagtgatt
     1681 ggtctattca ccatccccct gctgtaccgg cagcaccagg ctcagatcga ccaatatgtg
     1741 gggttggtga ccaatcagtt gagccacatc aaagctaaga tccgagctaa aatcccaggg
     1801 accggagccc tggcctctgc agcagccgca gtctccggat ccaaagccaa agccgaatga
     1861 gaacggtgtc tctgcccgca ggacgcctgc ccccagcccc cgcagccctc tggccccctc
     1921 catctcttgt ccgttcccac ccacccccct cctcggcccg agccttttcc cggtgggtgt
     1981 caggatcact cccactaggg actctgcgct aattacctga gcgaccagga ctacatttcc
     2041 caagaggctc tgctccagga gtccaggaaa gacgaggcac cttggccgcg gggcctgctg
     2101 ggacttgtag ttgcctagac agggcaccac cctgcacttc cggacccgcc gctggaggcg
     2161 ccgtgaggcg ttggtgtctc ctggatgcta ctagccccaa cgccggggct ttgcatgggg
     2221 cccaggggag gcctgagctt ggatttacac tgtaataaag actcctgtgg aaaa
//
