LOCUS       NM_145307               6886 bp    mRNA    linear   PRI 11-SEP-2024
DEFINITION  Homo sapiens rhotekin 2 (RTKN2), transcript variant 1, mRNA.
ACCESSION   NM_145307
VERSION     NM_145307.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6886)
  AUTHORS   Chen,J., Wang,H., Xu,J., Chen,E., Meng,Q., Wang,J., Xiang,H.,
            Zhou,W., Shan,G., Ju,Z. and Song,Z.
  TITLE     CircZFR promotes colorectal cancer progression via stabilizing
            BCLAF1 and regulating the miR-3127-5p/RTKN2 axis
  JOURNAL   Sci China Life Sci 67 (9), 1881-1898 (2024)
   PUBMED   38805063
  REMARK    GeneRIF: CircZFR promotes colorectal cancer progression via
            stabilizing BCLAF1 and regulating the miR-3127-5p/RTKN2 axis.
REFERENCE   2  (bases 1 to 6886)
  AUTHORS   Zhang,X., Wang,J. and Li,H.
  TITLE     RTKN2 knockdown alleviates the malignancy of breast cancer cells by
            regulating the Wnt/beta-catenin pathway
  JOURNAL   Sci Rep 13 (1), 23023 (2023)
   PUBMED   38155217
  REMARK    GeneRIF: RTKN2 knockdown alleviates the malignancy of breast cancer
            cells by regulating the Wnt/beta-catenin pathway.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6886)
  AUTHORS   Wang,N. and Wang,J.
  TITLE     RTKN2 Inhibits the Growth, Migration, Invasion and Glycolysis of
            Lung Adenocarcinoma Cells by Inactivating the NF-kappaB Signalling
            Pathway
  JOURNAL   Biochem Genet 61 (5), 2135-2148 (2023)
   PUBMED   36952123
  REMARK    GeneRIF: RTKN2 Inhibits the Growth, Migration, Invasion and
            Glycolysis of Lung Adenocarcinoma Cells by Inactivating the
            NF-kappaB Signalling Pathway.
REFERENCE   4  (bases 1 to 6886)
  AUTHORS   Huang,G., Liang,M., Liu,H., Huang,J., Li,P., Wang,C., Zhang,Y.,
            Lin,Y. and Jiang,X.
  TITLE     CircRNA hsa_circRNA_104348 promotes hepatocellular carcinoma
            progression through modulating miR-187-3p/RTKN2 axis and activating
            Wnt/beta-catenin pathway
  JOURNAL   Cell Death Dis 11 (12), 1065 (2020)
   PUBMED   33311442
  REMARK    GeneRIF: CircRNA hsa_circRNA_104348 promotes hepatocellular
            carcinoma progression through modulating miR-187-3p/RTKN2 axis and
            activating Wnt/beta-catenin pathway.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6886)
  AUTHORS   Wang,X., Zhang,L., Wang,W., Wang,Y., Chen,Y., Xie,R., Li,X. and
            Wang,Y.
  TITLE     Rhotekin 2 silencing inhibits proliferation and induces apoptosis
            in human osteosarcoma cells
  JOURNAL   Biosci Rep 38 (6) (2018)
   PUBMED   30389712
  REMARK    GeneRIF: hese results suggested that RTKN2 is involved in the
            progression of human osteosarcoma, and may be a potential
            therapeutic target.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6886)
  AUTHORS   Myouzen,K., Kochi,Y., Okada,Y., Terao,C., Suzuki,A., Ikari,K.,
            Tsunoda,T., Takahashi,A., Kubo,M., Taniguchi,A., Matsuda,F.,
            Ohmura,K., Momohara,S., Mimori,T., Yamanaka,H., Kamatani,N.,
            Yamada,R., Nakamura,Y. and Yamamoto,K.
  TITLE     Functional variants in NFKBIE and RTKN2 involved in activation of
            the NF-kappaB pathway are associated with rheumatoid arthritis in
            Japanese
  JOURNAL   PLoS Genet 8 (9), e1002949 (2012)
   PUBMED   23028356
  REMARK    GeneRIF: we identified two gene loci associated with rheumatoid
            arthritis susceptibility- NFKBIE and RTKN2
REFERENCE   7  (bases 1 to 6886)
  AUTHORS   Newton-Cheh,C., Johnson,T., Gateva,V., Tobin,M.D., Bochud,M.,
            Coin,L., Najjar,S.S., Zhao,J.H., Heath,S.C., Eyheramendy,S.,
            Papadakis,K., Voight,B.F., Scott,L.J., Zhang,F., Farrall,M.,
            Tanaka,T., Wallace,C., Chambers,J.C., Khaw,K.T., Nilsson,P., van
            der Harst,P., Polidoro,S., Grobbee,D.E., Onland-Moret,N.C.,
            Bots,M.L., Wain,L.V., Elliott,K.S., Teumer,A., Luan,J., Lucas,G.,
            Kuusisto,J., Burton,P.R., Hadley,D., McArdle,W.L., Brown,M.,
            Dominiczak,A., Newhouse,S.J., Samani,N.J., Webster,J., Zeggini,E.,
            Beckmann,J.S., Bergmann,S., Lim,N., Song,K., Vollenweider,P.,
            Waeber,G., Waterworth,D.M., Yuan,X., Groop,L., Orho-Melander,M.,
            Allione,A., Di Gregorio,A., Guarrera,S., Panico,S., Ricceri,F.,
            Romanazzi,V., Sacerdote,C., Vineis,P., Barroso,I., Sandhu,M.S.,
            Luben,R.N., Crawford,G.J., Jousilahti,P., Perola,M., Boehnke,M.,
            Bonnycastle,L.L., Collins,F.S., Jackson,A.U., Mohlke,K.L.,
            Stringham,H.M., Valle,T.T., Willer,C.J., Bergman,R.N., Morken,M.A.,
            Doring,A., Gieger,C., Illig,T., Meitinger,T., Org,E., Pfeufer,A.,
            Wichmann,H.E., Kathiresan,S., Marrugat,J., O'Donnell,C.J.,
            Schwartz,S.M., Siscovick,D.S., Subirana,I., Freimer,N.B.,
            Hartikainen,A.L., McCarthy,M.I., O'Reilly,P.F., Peltonen,L.,
            Pouta,A., de Jong,P.E., Snieder,H., van Gilst,W.H., Clarke,R.,
            Goel,A., Hamsten,A., Peden,J.F., Seedorf,U., Syvanen,A.C.,
            Tognoni,G., Lakatta,E.G., Sanna,S., Scheet,P., Schlessinger,D.,
            Scuteri,A., Dorr,M., Ernst,F., Felix,S.B., Homuth,G., Lorbeer,R.,
            Reffelmann,T., Rettig,R., Volker,U., Galan,P., Gut,I.G.,
            Hercberg,S., Lathrop,G.M., Zelenika,D., Deloukas,P., Soranzo,N.,
            Williams,F.M., Zhai,G., Salomaa,V., Laakso,M., Elosua,R.,
            Forouhi,N.G., Volzke,H., Uiterwaal,C.S., van der Schouw,Y.T.,
            Numans,M.E., Matullo,G., Navis,G., Berglund,G., Bingham,S.A.,
            Kooner,J.S., Connell,J.M., Bandinelli,S., Ferrucci,L., Watkins,H.,
            Spector,T.D., Tuomilehto,J., Altshuler,D., Strachan,D.P., Laan,M.,
            Meneton,P., Wareham,N.J., Uda,M., Jarvelin,M.R., Mooser,V.,
            Melander,O., Loos,R.J., Elliott,P., Abecasis,G.R., Caulfield,M. and
            Munroe,P.B.
  CONSRTM   Wellcome Trust Case Control Consortium
  TITLE     Genome-wide association study identifies eight loci associated with
            blood pressure
  JOURNAL   Nat Genet 41 (6), 666-676 (2009)
   PUBMED   19430483
REFERENCE   8  (bases 1 to 6886)
  AUTHORS   Grupe,A., Li,Y., Rowland,C., Nowotny,P., Hinrichs,A.L., Smemo,S.,
            Kauwe,J.S., Maxwell,T.J., Cherny,S., Doil,L., Tacey,K., van
            Luchene,R., Myers,A., Wavrant-De Vrieze,F., Kaleem,M.,
            Hollingworth,P., Jehu,L., Foy,C., Archer,N., Hamilton,G.,
            Holmans,P., Morris,C.M., Catanese,J., Sninsky,J., White,T.J.,
            Powell,J., Hardy,J., O'Donovan,M., Lovestone,S., Jones,L.,
            Morris,J.C., Thal,L., Owen,M., Williams,J. and Goate,A.
  TITLE     A scan of chromosome 10 identifies a novel locus showing strong
            association with late-onset Alzheimer disease
  JOURNAL   Am J Hum Genet 78 (1), 78-88 (2006)
   PUBMED   16385451
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   9  (bases 1 to 6886)
  AUTHORS   Collier,F.M., Gregorio-King,C.C., Gough,T.J., Talbot,C.D.,
            Walder,K. and Kirkland,M.A.
  TITLE     Identification and characterization of a lymphocytic Rho-GTPase
            effector: rhotekin-2
  JOURNAL   Biochem Biophys Res Commun 324 (4), 1360-1369 (2004)
   PUBMED   15504364
  REMARK    GeneRIF: Cloning and characterization of gene expression of
            rhotekin-2.
REFERENCE   10 (bases 1 to 6886)
  AUTHORS   Gregorio-King,C.C., Gough,T., Van Der Meer,G.J., Hosking,J.B.,
            Waugh,C.M., McLeod,J.L., Collier,F.M. and Kirkland,M.A.
  TITLE     Mechanisms of resistance to the cytotoxic effects of oxysterols in
            human leukemic cells
  JOURNAL   J Steroid Biochem Mol Biol 88 (3), 311-320 (2004)
   PUBMED   15120425
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC141821.1, BX113509.1, AY150309.1, AL607150.10 and BG029885.1.
            
            On Nov 23, 2018 this sequence version replaced NM_145307.3.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY150309.1, DRR138524.389098.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000373789.8/ ENSP00000362894.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-65                BC141821.1         20-84
            66-137              BX113509.1         37-108
            138-6778            AY150309.1         1-6641
            6779-6849           AL607150.10        52688-52758         c
            6850-6886           BG029885.1         608-644
FEATURES             Location/Qualifiers
     source          1..6886
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10q21.2"
     gene            1..6886
                     /gene="RTKN2"
                     /gene_synonym="bA531F24.1; PLEKHK1"
                     /note="rhotekin 2"
                     /db_xref="GeneID:219790"
                     /db_xref="HGNC:HGNC:19364"
                     /db_xref="MIM:618450"
     exon            1..294
                     /gene="RTKN2"
                     /gene_synonym="bA531F24.1; PLEKHK1"
                     /inference="alignment:Splign:2.1.0"
     CDS             235..2064
                     /gene="RTKN2"
                     /gene_synonym="bA531F24.1; PLEKHK1"
                     /note="isoform 1 is encoded by transcript variant 1; PH
                     domain-containing family K member 1; pleckstrin homology
                     domain-containing family K member 1"
                     /codon_start=1
                     /product="rhotekin-2 isoform 1"
                     /protein_id="NP_660350.2"
                     /db_xref="CCDS:CCDS7263.1"
                     /db_xref="GeneID:219790"
                     /db_xref="HGNC:HGNC:19364"
                     /db_xref="MIM:618450"
                     /translation="MEGPSLRGPALRLAGLPTQQDCNIQEKIDLEIRMREGIWKLLSL
                     STQKDQVLHAVKNLMVCNARLMAYTSELQKLEEQIANQTGRCDVKFESKERTACKGKI
                     AISDIRIPLMWKDSDHFSNKERSRRYAIFCLFKMGANVFDTDVVNVDKTITDICFENV
                     TIFNEAGPDFQIKVEVYSCCTEESSITNTPKKLAKKLKTSISKATGKKISSVLQEEDD
                     EMCLLLSSAVFGVKYNLLAHTTLTLESAEDSFKTHNLSINGNEESSFWLPLYGNMCCR
                     LVAQPACMAEDAFAGFLNQQQMVEGLISWRRLYCVLRGGKLYCFYSPEEIEAKVEPAL
                     VVPINKETRIRAMDKDAKKRIHNFSVINPVPGQAITQIFAVDNREDLQKWMEAFWQHF
                     FDLSQWKHCCEELMKIEIMSPRKPPLFLTKEATSVYHDMSIDSPMKLESLTDIIQKKI
                     EETNGQFLIGQHEESLPPPWATLFDGNHQMVIQKKVLYPASEPLHDEKGKKRQAPLPP
                     SDKLPFSLKSQSNTDQLVKDNWGKTSVSQTSSLDTKLSTLMHHLQKPMAAPRKLLPAR
                     RNRLSDGEHTDTKTNFEAKPVPAPRQKSIKDILDPRSWLQAQV"
     exon            295..491
                     /gene="RTKN2"
                     /gene_synonym="bA531F24.1; PLEKHK1"
                     /inference="alignment:Splign:2.1.0"
     exon            492..550
                     /gene="RTKN2"
                     /gene_synonym="bA531F24.1; PLEKHK1"
                     /inference="alignment:Splign:2.1.0"
     exon            551..604
                     /gene="RTKN2"
                     /gene_synonym="bA531F24.1; PLEKHK1"
                     /inference="alignment:Splign:2.1.0"
     exon            605..722
                     /gene="RTKN2"
                     /gene_synonym="bA531F24.1; PLEKHK1"
                     /inference="alignment:Splign:2.1.0"
     exon            723..920
                     /gene="RTKN2"
                     /gene_synonym="bA531F24.1; PLEKHK1"
                     /inference="alignment:Splign:2.1.0"
     exon            921..1015
                     /gene="RTKN2"
                     /gene_synonym="bA531F24.1; PLEKHK1"
                     /inference="alignment:Splign:2.1.0"
     exon            1016..1122
                     /gene="RTKN2"
                     /gene_synonym="bA531F24.1; PLEKHK1"
                     /inference="alignment:Splign:2.1.0"
     exon            1123..1254
                     /gene="RTKN2"
                     /gene_synonym="bA531F24.1; PLEKHK1"
                     /inference="alignment:Splign:2.1.0"
     exon            1255..1420
                     /gene="RTKN2"
                     /gene_synonym="bA531F24.1; PLEKHK1"
                     /inference="alignment:Splign:2.1.0"
     exon            1421..1528
                     /gene="RTKN2"
                     /gene_synonym="bA531F24.1; PLEKHK1"
                     /inference="alignment:Splign:2.1.0"
     exon            1529..6886
                     /gene="RTKN2"
                     /gene_synonym="bA531F24.1; PLEKHK1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5952..5957
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RTKN2"
                     /gene_synonym="bA531F24.1; PLEKHK1"
                     /note="hexamer: ATTAAA"
     polyA_site      5973
                     /gene="RTKN2"
                     /gene_synonym="bA531F24.1; PLEKHK1"
                     /note="major polyA site"
     regulatory      6754..6759
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RTKN2"
                     /gene_synonym="bA531F24.1; PLEKHK1"
                     /note="hexamer: AATAAA"
     polyA_site      6778
                     /gene="RTKN2"
                     /gene_synonym="bA531F24.1; PLEKHK1"
                     /note="major polyA site"
     regulatory      6869..6874
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RTKN2"
                     /gene_synonym="bA531F24.1; PLEKHK1"
                     /note="hexamer: AATAAA"
     polyA_site      6886
                     /gene="RTKN2"
                     /gene_synonym="bA531F24.1; PLEKHK1"
ORIGIN      
        1 gccccgccgc gctagagcct ggggtctcgg caacttccgg cggcgggagc tgcagagcgc
       61 aaggcgcgcc cactgcgcgt gcgcttcggc ccggctcctc ctgcgccccc ggcccctgcg
      121 actgggactt ggtacggccg ggcggttggc gtcctctgcg gctcctgcca ggggcgggct
      181 tttcaaatct tccctttgaa ggagtggcga cggcccggac agttcgcgtt ggagatggag
      241 gggccgagcc tgaggggtcc tgcgctccgc ctggcggggc ttcccaccca gcaggactgc
      301 aacattcaag aaaaaataga cttagaaatt cgaatgcgag aaggaatatg gaaactcctt
      361 tctctgagca ctcagaaaga tcaagtttta catgcagtta agaatctcat ggtgtgcaat
      421 gctcgactaa tggcctatac atcggagcta cagaaattag aagaacagat tgcaaatcag
      481 actggaagat gtgatgtgaa atttgaaagt aaagaacgaa cagcatgtaa aggaaagatt
      541 gccatatcag atattcgaat accactaatg tggaaagact ctgatcactt cagcaataaa
      601 gaacgatcac gacgctatgc cattttttgt ttattcaaaa tgggagctaa tgtgtttgat
      661 actgatgtgg tgaatgtgga taaaacaatc acagatatat gttttgaaaa tgtaaccata
      721 tttaatgaag cagggccaga ctttcagata aaggtggaag tgtatagttg ctgtacagaa
      781 gaatcttcta taaccaacac accaaaaaag ctagctaaga aactcaagac atctataagt
      841 aaagctacag gaaagaaaat aagttcagtg cttcaagaag aggatgatga aatgtgcttg
      901 ctcctcagct ctgctgtttt tggtgtaaag tataatttgc tagctcacac taccctaacc
      961 ttggaaagtg ctgaggatag tttcaagacc cataatctgt ctattaatgg aaatgaggag
     1021 tcttcatttt ggcttcccct gtatggcaac atgtgctgcc gactagttgc ccagccagct
     1081 tgtatggctg aggatgcatt tgcaggattt cttaatcagc agcaaatggt agaaggtctg
     1141 attagttgga gaaggttgta ttgtgttttg cgaggaggta aactctattg tttttacagt
     1201 ccagaggaaa ttgaagctaa agtggaacca gctttggtag tacccattaa caaggaaacc
     1261 agaatccggg caatggataa ggatgccaag aaaagaatcc ataatttctc tgtcatcaat
     1321 cctgttcctg gacaagctat aactcagatt tttgcagttg acaatagaga agatcttcag
     1381 aagtggatgg aagccttctg gcagcatttc tttgatctta gccaatggaa gcactgttgt
     1441 gaagaactta tgaaaattga gattatgtca ccacggaaac cacctttgtt cttgacaaaa
     1501 gaggcaacct cagtctacca tgatatgagc attgattcac ctatgaaact tgaaagttta
     1561 acggatataa tacaaaaaaa aattgaagag acaaatgggc agttccttat tggtcagcat
     1621 gaagaatcct taccacctcc ttgggccaca ctctttgatg gtaaccatca aatggtcatc
     1681 cagaaaaagg tactgtatcc tgcaagtgag ccattacatg atgaaaaagg gaaaaagaga
     1741 caagctcccc ttcctccttc tgataaactt ccattcagct taaaatcaca gagtaacaca
     1801 gatcaattgg ttaaggacaa ctggggaaaa acaagtgtat ctcagacatc gtctttggat
     1861 accaaactat caactctaat gcatcactta cagaaaccaa tggctgctcc tcgaaaactt
     1921 ctgcctgcca ggagaaatag attaagtgat ggtgagcaca cagacactaa aaccaatttt
     1981 gaagccaagc cagtgccagc tccaaggcag aaatccatca aagacattct ggaccctaga
     2041 tcatggctgc aggcacaagt atagaaaact cttaatgata aaacatttaa caaaatctat
     2101 aactgtgaag cttaattcag aggcattata gaataatgtg taattatata ggtaaatata
     2161 gtgatttata ccaataatta ataagctatt aggtatagaa aaacatttta taatgaagaa
     2221 gtggatttct cttttactcc tgatttgcag aatgtaagtg atttctccag cagtgtgcaa
     2281 gaggcagttt ctagacaata tcctaattgt tggaagggtt aatttattcc tgaattagta
     2341 ccaggcagtg aataagccct aggcagaata aagcattaaa atttcttttg gggagagact
     2401 gtctagtaat ttaaaatgtg ttaaatatga accatattct cattgtgaga attttaatac
     2461 taatggaatg tttcaaaggt tttattcaaa ccacaaatta tagaatccac ccaagaaaat
     2521 tttcttagca ttctggaatt ctcatctaat ggttcactgg ctctcatcta ttgtttaact
     2581 cactagttta taggcctgac cttttaaatt cctgggataa attttagaat attaaattct
     2641 tcatctttaa agggaatttg taatgtgcta ttcaaacttg tttccttgat gagccctgaa
     2701 atactgttga cattaacttt cataaatgaa gcataacact tactagttta gagaaatggt
     2761 agatgaaatg ctctttacat tcaattttct gtgtgtggtg ctaatttcca ttgtgacttt
     2821 ggaatttagg gaagtgagta gctgatcaac accttaaaat tcatttaata cttatttaaa
     2881 aagaagatga gagattcaaa aatatcagaa aagttggcca tggtaaataa ttttcagaat
     2941 tcttttttaa aattttaatt agatttcctt ttcctagtaa gagttgatgt ctgctagtgg
     3001 tatttttcat taaatgtcat attcttacaa cagtgacatt ggaatcattt ccataggagg
     3061 taatttccat agcaactaat catagtgacc caaacaggat tataaagtca agtgaggaat
     3121 aaatagcaga aaaatacctt aaaggaacaa aaaatccatt ttttataata atgctttttg
     3181 taaacaagag cagaacagtt ttaaaaatct aattagaatt tccaaataaa acagtttcca
     3241 taaggtgtca gcaacagcac taaaaatctc aaatcacact gaaccctaaa tgtcattgca
     3301 agaaataaaa atttaagaat taaattgctg ccctgtgtat aatctcttga gatcttttta
     3361 aagctagcaa gaactctatt tggatcagaa ctacattatt acaaaacaaa atgaacactt
     3421 gaccaaaaaa ccacattaat ttaaatggag cctttcatta ttttcagata gcggggtctt
     3481 ttccatttat agagaatgat gtaactgcca ggacactttt gtgtttgccc aggactccta
     3541 gtaagaattg aatcttgagg gagtaatgat aacaaaaggt ttcttgtgac tggtaacatc
     3601 aggacagaca gagtttttgg tgagaagaaa gtactatatg cctgcttgat agcatgtaga
     3661 gatcatcaca tgtatggaaa ccttaaaaag taaacttatt ttttctaact agaaggctct
     3721 cataagtttc tgttatgatt gccatttcaa taaatgacat taaaaatcta aaggatatta
     3781 ttgtttgaat ttttgggtta ttttttaaaa cttcattgct gtttttgaca gcatttgtga
     3841 ctgtcattgc cactaaatta ttgagctaga agatgctgca gggaaaattg ttttgaagct
     3901 ggcatctaag cattcttttt tttttcctag ttgatgcctt cctttattac aaatcagtat
     3961 taaaacttcc agattatatt cttgtcattt ttcctcatgc agataacagc aggaaggact
     4021 taaggtgtat aagaaaaaga aagtattcat acagaaatta cctagcagtt gtgccctcag
     4081 agtagaactt tgaaacagga ctgaagccct ttttacctag aaccatttaa aagcctgagc
     4141 aaggaccacc accccctccc caccaaatta ttcggtctct ttctgggggg cccagcttac
     4201 tgtatgttac aagtctttga aatgggtctg gtgtcttcag ctcctgaaat gctaatttac
     4261 cccttaccta aggagtgtag ttataataca gagttggttt ccttccttcc ttccttcctt
     4321 ccttccttcc ttccttcctt ccttccttcc ttccttcctt ccttcattcc ctctgtccgt
     4381 ctctccttcc ctccttccct ccttccttcc tttttgtctt tgtttctttc tctggccccc
     4441 tgtcctctct ctctgtctca tccccttttc ccactttcct tctgtctctc cttcctccct
     4501 tcctttacag tttaaaaaaa aaatctaaaa atagttaagc actgtgtcaa agagtgtttc
     4561 aaaagaattg tttgttttct catttcttaa accagaagtt tatgacaaat aaatatgcca
     4621 tgtaatatga aagctgcttc agctttttat gagctttcac attccttgtt tgtcctttta
     4681 atttgtttct tgatatatga agactataaa cttgaaacaa agagttagct gtcagctctt
     4741 gataatataa ttggggaaac agaatagata ttttatgata tgataattct gatgatctaa
     4801 agataatgtc ttagacaatt acctttacat ttcaagctct gggatataaa ttacgtagtc
     4861 agcattaaga tagtattatt tttaattgct actaagtggt aacttttgta ttttaatatc
     4921 ttgcaagggc aaagaaggca tttttaaaat tctaaatgca cacctcctaa ggtaaatagt
     4981 ctttcaaaaa tatcaggcgt gccaactctt gctgagtttt gttaaatcct ccttaactgt
     5041 ctgcagtgcc ccctgacttc ttttaaatgg atgtgaatat atgtggaaaa ctaaatccat
     5101 ttattataaa tgccttatct ttaccctttg ggatcttact aaatgcattt tacctgctac
     5161 tatttattaa aaatggaagc tgtcttgaat tagagatacc tcacgtaagg taggttaagc
     5221 agtcaagtat ttctttaggc ttatgttcag cacctcagcc ccctactgag aagttaatta
     5281 attcagtcca aaatgaaatt atagggcagt ttggtgcaat ttatgttaaa tgccatttga
     5341 atgtaatcac ctgtgttacc acgaatgagg cacaactata ctacatggac agagtactgc
     5401 attgcaccat gagcaagaac tgctctgcaa gaaaaatatc tgtccctgtg gtctgaattt
     5461 atcaaaactc attttcttgt taaagagtga attattgaat agtaattaaa ctttaactga
     5521 tgaccattta tattctagta gccatcttca ctgttagtct taaattataa tgtaaagttg
     5581 acattttccc cttacatttc aaaggcatca ctatttttaa atgtttacat atgtttacat
     5641 gtttgtgtgc aactgcttca ttgctattaa tagttaaatg aaatctgcat attctggatc
     5701 atagatgttc ttagttaact ctgggaaaga tgttaccttt gggtttttaa aagacaagtc
     5761 atcatttgaa aatatattag ggtgtgttga ttttctgaca tgcttgtgta attggctttg
     5821 tttataattt ttatttttta atatgggtca gacttttgga ttaaaaagta ttatatcatg
     5881 gacatacatt ttttaaagta ccaaaggtat ttggtaagcg agcccaagtc atgtaattga
     5941 aacttctgta cattaaaaga actgattcat gaagacgttt tctaactttt tctctttaag
     6001 tgctcctcta gactagactg attgaagtag acagtgtggt ggaatcagtt taatcagaaa
     6061 aagacatacc aagtagctgg tgctaattat tataagacta acattctgat aagccatggt
     6121 ataattaaca ttattaaaat gtttacatat aatccttctt aaagtatact cttttaaaaa
     6181 tccattgaca taaccttact tttagtttag tgatccagaa tttccccaga gcttaagcca
     6241 ctgcagtaaa ttaggtacgt aggatattca gtcgctacta gccacaagga gtctccttat
     6301 tttaatgtac ctccctcagt actttattcc tgcagagcgc ctcagagtgg gggagagaaa
     6361 tgagcaatcc tggctcagtt ggattatttc agcattttat ttctaaatct gtagtgtgat
     6421 cagaaatatt taaaattaaa aaaatacttt acagagagag gcctactaat caatgtgctt
     6481 agagaaacaa actaccttta cattcaatta acaacaaaaa aagttacaga aattttaaca
     6541 aacatttttc tttatctgag tatgggagaa aatactaatg ctgacacgtt taataattag
     6601 aaaagatcat tccagatctt atggaaaatt gtgttttaaa ttccagcagt ttgctcttga
     6661 gacatggtaa tctcttaatg taactactaa aggagaggta gattgaaaag atcttgtata
     6721 tacataatta tgtcagatgg ttcaagatat caaaataaat tgttttgttg aaaatgaata
     6781 ctttttggtg tcttgcttaa aaatattttt atattttgtt tatctactca aaattatgaa
     6841 tggatgcatt tttgtagaac ttaatcttaa taaacattta actgaa
//
