LOCUS       NM_001015055            2221 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens rhotekin (RTKN), transcript variant 1, mRNA.
ACCESSION   NM_001015055
VERSION     NM_001015055.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Swaney,D.L., Ramms,D.J., Wang,Z., Park,J., Goto,Y., Soucheray,M.,
            Bhola,N., Kim,K., Zheng,F., Zeng,Y., McGregor,M., Herrington,K.A.,
            O'Keefe,R., Jin,N., VanLandingham,N.K., Foussard,H., Von Dollen,J.,
            Bouhaddou,M., Jimenez-Morales,D., Obernier,K., Kreisberg,J.F.,
            Kim,M., Johnson,D.E., Jura,N., Grandis,J.R., Gutkind,J.S.,
            Ideker,T. and Krogan,N.J.
  TITLE     A protein network map of head and neck cancer reveals PIK3CA mutant
            drug sensitivity
  JOURNAL   Science 374 (6563), eabf2911 (2021)
   PUBMED   34591642
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Zhou,J., Zhang,Y., Qi,Y., Yu,D., Shao,Q. and Liang,J.
  TITLE     MicroRNA-152 inhibits tumor cell growth by directly targeting RTKN
            in hepatocellular carcinoma
  JOURNAL   Oncol Rep 37 (2), 1227-1234 (2017)
   PUBMED   27922690
  REMARK    GeneRIF: RTKN is upregulated in hepatocellular
            carcinoma.MicroRNA-152 inhibits tumor cell growth by directly
            targeting RTKN in hepatocellular carcinoma.
REFERENCE   3  (bases 1 to 2221)
  AUTHORS   Li,B., Chen,P., Chang,Y., Qi,J., Fu,H. and Guo,H.
  TITLE     Let-7a inhibits tumor cell growth and metastasis by directly
            targeting RTKN in human colon cancer
  JOURNAL   Biochem Biophys Res Commun 478 (2), 739-745 (2016)
   PUBMED   27498032
  REMARK    GeneRIF: Let-7a microRNA inhibits tumor cell growth and metastasis
            by directly targeting RTKN in human colon cancer.
REFERENCE   4  (bases 1 to 2221)
  AUTHORS   Zhang,W., Liang,Z. and Li,J.
  TITLE     Inhibition of rhotekin exhibits antitumor effects in lung cancer
            cells
  JOURNAL   Oncol Rep 35 (5), 2529-2534 (2016)
   PUBMED   26935528
  REMARK    GeneRIF: The RTKN protein is associated with lung cancer in clinic
            samples and exerts anticancer activity in lung adenocarcinoma cells
            through inhibiting cell cycle progression and the DNA replication
            machinery.
REFERENCE   5  (bases 1 to 2221)
  AUTHORS   Qu,G.Q., Lu,Y.M., Liu,Y.F., Liu,Y., Chen,W.X., Liao,X.H. and
            Kong,W.M.
  TITLE     Effect of RTKN on progression and metastasis of colon cancer in
            vitro
  JOURNAL   Biomed Pharmacother 74, 117-123 (2015)
   PUBMED   26349972
  REMARK    GeneRIF: RTKN could affect the proliferation and metastasis of
            colon cancer by reducing expression of MCM2/3/5, CDK1/2 and PCNA
REFERENCE   6  (bases 1 to 2221)
  AUTHORS   Katoh,H. and Negishi,M.
  TITLE     RhoG activates Rac1 by direct interaction with the Dock180-binding
            protein Elmo
  JOURNAL   Nature 424 (6947), 461-464 (2003)
   PUBMED   12879077
REFERENCE   7  (bases 1 to 2221)
  AUTHORS   Sharfe,N., Freywald,A., Toro,A., Dadi,H. and Roifman,C.
  TITLE     Ephrin stimulation modulates T cell chemotaxis
  JOURNAL   Eur J Immunol 32 (12), 3745-3755 (2002)
   PUBMED   12516569
REFERENCE   8  (bases 1 to 2221)
  AUTHORS   Reynaud,C., Fabre,S. and Jalinot,P.
  TITLE     The PDZ protein TIP-1 interacts with the Rho effector rhotekin and
            is involved in Rho signaling to the serum response element
  JOURNAL   J Biol Chem 275 (43), 33962-33968 (2000)
   PUBMED   10940294
REFERENCE   9  (bases 1 to 2221)
  AUTHORS   Fu,Q., Yu,L., Liu,Q., Zhang,J., Zhang,H. and Zhao,S.
  TITLE     Molecular cloning, expression characterization, and mapping of a
            novel putative inhibitor of rho GTPase activity, RTKN, to
            D2S145-D2S286
  JOURNAL   Genomics 66 (3), 328-332 (2000)
   PUBMED   10873388
REFERENCE   10 (bases 1 to 2221)
  AUTHORS   Jang,W., Weber,J.S., Harkins,E.B. and Meisler,M.H.
  TITLE     Localization of the rhotekin gene RTKN on the physical maps of
            mouse chromosome 6 and human chromosome 2p13 and exclusion as a
            candidate for mnd2 and LGMD2B
  JOURNAL   Genomics 40 (3), 506-507 (1997)
   PUBMED   9073523
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC005041.2, BG470092.1,
            BC004558.2 and BQ574374.1.
            
            On May 17, 2019 this sequence version replaced NM_001015055.1.
            
            Summary: This gene encodes a scaffold protein that interacts with
            GTP-bound Rho proteins. Binding of this protein inhibits the GTPase
            activity of Rho proteins. This protein may interfere with the
            conversion of active, GTP-bound Rho to the inactive GDP-bound form
            by RhoGAP. Rho proteins regulate many important cellular processes,
            including cytokinesis, transcription, smooth muscle contraction,
            cell growth and transformation. Dysregulation of the Rho signal
            transduction pathway has been implicated in many forms of cancer.
            Alternative splicing results in multiple transcript variants
            encoding different isoforms. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7346977.1193810.1,
                                           SRR7346977.966936.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000272430.10/ ENSP00000272430.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-4                 AC005041.2         93362-93365
            5-58                BG470092.1         36-89
            59-2172             BC004558.2         1-2114
            2173-2195           BQ574374.1         18-40               c
            2196-2221           AC005041.2         109439-109464
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2p13.1"
     gene            1..2221
                     /gene="RTKN"
                     /gene_synonym="RTKN-1"
                     /note="rhotekin"
                     /db_xref="GeneID:6242"
                     /db_xref="HGNC:HGNC:10466"
                     /db_xref="MIM:602288"
     exon            1..232
                     /gene="RTKN"
                     /gene_synonym="RTKN-1"
                     /inference="alignment:Splign:2.1.0"
     CDS             122..1813
                     /gene="RTKN"
                     /gene_synonym="RTKN-1"
                     /note="isoform a is encoded by transcript variant 1"
                     /codon_start=1
                     /product="rhotekin isoform a"
                     /protein_id="NP_001015055.1"
                     /db_xref="CCDS:CCDS33226.1"
                     /db_xref="GeneID:6242"
                     /db_xref="HGNC:HGNC:10466"
                     /db_xref="MIM:602288"
                     /translation="MFSRNHRSRVTVARGSALEMEFKRGRFRLSLFSDLPEDTELQRK
                     LDHEIRMREGACKLLAACSQREQALEATKSLLVCNSRILSYMGELQRRKEAQVLGKTS
                     RRPSDSGPPAERSPCRGRVCISDLRIPLMWKDTEYFKNKGDLHRWAVFLLLQLGEHIQ
                     DTEMILVDRTLTDISFQSNVLFAEAGPDFELRLELYGACVEEEGALTGGPKRLATKLS
                     SSLGRSSGRRVRASLDSAGGSGSSPILLPTPVVGGPRYHLLAHTTLTLAAVQDGFRTH
                     DLTLASHEENPAWLPLYGSVCCRLAAQPLCMTQPTASGTLRVQQAGEMQNWAQVHGVL
                     KGTNLFCYRQPEDADTGEEPLLTIAVNKETRVRAGELDQALGRPFTLSISNQYGDDEV
                     THTLQTESREALQSWMEALWQLFFDMSQWKQCCDEIMKIETPAPRKPPQALAKQGSLY
                     HEMAIEPLDDIAAVTDILTQREGARLETPPPWLAMFTDQPALPNPCSPASVAPAPDWT
                     HPLPWGRPRTFSLDAVPPDHSPRARSVAPLPPQRSPRTRGLCSKGQPRTWLQSPV"
     exon            233..432
                     /gene="RTKN"
                     /gene_synonym="RTKN-1"
                     /inference="alignment:Splign:2.1.0"
     exon            433..494
                     /gene="RTKN"
                     /gene_synonym="RTKN-1"
                     /inference="alignment:Splign:2.1.0"
     exon            495..548
                     /gene="RTKN"
                     /gene_synonym="RTKN-1"
                     /inference="alignment:Splign:2.1.0"
     exon            549..666
                     /gene="RTKN"
                     /gene_synonym="RTKN-1"
                     /inference="alignment:Splign:2.1.0"
     exon            667..876
                     /gene="RTKN"
                     /gene_synonym="RTKN-1"
                     /inference="alignment:Splign:2.1.0"
     exon            877..971
                     /gene="RTKN"
                     /gene_synonym="RTKN-1"
                     /inference="alignment:Splign:2.1.0"
     exon            972..1078
                     /gene="RTKN"
                     /gene_synonym="RTKN-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1079..1207
                     /gene="RTKN"
                     /gene_synonym="RTKN-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1208..1376
                     /gene="RTKN"
                     /gene_synonym="RTKN-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1377..1481
                     /gene="RTKN"
                     /gene_synonym="RTKN-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1482..2221
                     /gene="RTKN"
                     /gene_synonym="RTKN-1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2154..2159
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RTKN"
                     /gene_synonym="RTKN-1"
                     /note="hexamer: ATTAAA"
     polyA_site      2173
                     /gene="RTKN"
                     /gene_synonym="RTKN-1"
                     /note="major polyA site"
ORIGIN      
        1 gcagcgggag attcccggcc ggactggcgg acgagcgtcg agagacagaa gccgaggaga
       61 ggaggctaag ccgggcggcg cggggagcgc actgagcagg caaagtcgca gggccgccag
      121 catgttctcc cgaaaccacc ggagccgggt caccgtggcc aggggctccg ccctggagat
      181 ggagttcaaa cgcggccgct tccgactcag cctcttcagc gacctgcccg aggacacgga
      241 gttgcagagg aagctagacc atgagatccg gatgagggaa ggggcctgta agctgctggc
      301 agcctgctcc cagcgagagc aggctctgga ggccaccaag agcctgctag tgtgcaacag
      361 ccgcatcctc agctacatgg gcgagctgca gcggcgcaag gaggcgcagg tgctggggaa
      421 gacaagccgg cggccttctg acagtggccc gcccgctgag cgctccccct gccgcggccg
      481 ggtctgcatc tctgacctcc ggattccact catgtggaag gacacagaat atttcaagaa
      541 caaaggtgac ttgcaccgct gggctgtgtt cctgctgctg cagctggggg aacacatcca
      601 ggacacagag atgatcctag tggacaggac cctcacagac atctcctttc agagcaatgt
      661 gctcttcgct gaggcggggc cagactttga actgcggtta gagctgtatg gggcctgtgt
      721 ggaagaagag ggggccctga ctggcggccc caagaggctt gccaccaaac tcagcagctc
      781 cctgggccgc tcctcaggga ggcgtgtccg ggcatcgctg gacagtgctg ggggttcagg
      841 gagcagtccc atcttgctcc ccaccccagt tgttggtggt cctcgttacc acctcttggc
      901 tcacaccaca ctcaccctgg cagcagtgca agatggattc cgcacacatg acctcaccct
      961 tgccagtcat gaggagaacc ctgcctggct gcccctttat ggtagcgtgt gttgccgtct
     1021 ggcagctcag cctctctgca tgactcagcc cactgcaagt ggtaccctca gggtgcagca
     1081 agctggggag atgcagaact gggcacaagt gcatggagtt ctgaaaggca caaacctctt
     1141 ctgttaccgg caacctgagg atgcagacac tggggaagag ccgctgctta ctattgctgt
     1201 caacaaggag actcgagtcc gggcagggga gctggaccag gctctaggac ggcccttcac
     1261 cctaagcatc agtaaccagt atggggatga tgaggtgaca cacacccttc agacagaaag
     1321 tcgggaagca ctgcagagct ggatggaggc tctgtggcag cttttctttg acatgagcca
     1381 atggaagcag tgctgtgatg aaatcatgaa aattgaaact cctgctcccc ggaaaccacc
     1441 ccaagcactg gcaaagcagg ggtccttgta ccatgagatg gctattgagc cgctggatga
     1501 catcgcagcg gtgacagaca tcctgaccca gcgggagggc gcaaggctgg agacaccccc
     1561 accctggctg gcaatgttta cagaccagcc tgccctgcct aacccctgct cgcctgcctc
     1621 agtggcccca gccccagact ggacccaccc cctgccctgg gggagacccc gaaccttttc
     1681 cctggatgct gtccccccag accactcccc tagggctcgc tcggttgccc ccctcccacc
     1741 tcagcgatcc ccacggacca gaggcctctg cagcaaaggc caacctcgca cttggctcca
     1801 gtcaccagtg tgagagagaa aggtgctggc ataggatctg cccagaagag aaaatgaccc
     1861 atgcgcagtt gggctctgga tacggcgctg tctatagcaa gttggccagt ctggcctcct
     1921 gttcctctgc tggacctggg gtaggctgca ggggtgggca gaagcccctc ttaaattgtg
     1981 gttgccatgg taccgaggga ctcattcctg gggctcgctg ggacctccct aaacccttcc
     2041 tggaagaaaa ctggaaccaa ctctgcccta cctccctgca ctaaccagct ttgaggatgg
     2101 cactgaagaa cccttggagc aaacatacct cccttgtgac tcccacatca accattaaag
     2161 ttatttaaca gcagccttca cctggctcct gaggacaggg tgcctctctc tgcctggtct
     2221 a
//
