LOCUS       NM_032784               4815 bp    mRNA    linear   PRI 09-OCT-2024
DEFINITION  Homo sapiens R-spondin 3 (RSPO3), mRNA.
ACCESSION   NM_032784
VERSION     NM_032784.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4815)
  AUTHORS   Li,H., Zhang,J., Yu,B., Yang,T., Liu,B., Li,F., Jin,X. and Li,Q.
  TITLE     RSPO3 regulates the radioresistance of Non-Small cell lung cancer
            cells via NLRP3 Inflammasome-Mediated pyroptosis
  JOURNAL   Radiother Oncol 200, 110528 (2024)
   PUBMED   39245068
  REMARK    GeneRIF: RSPO3 regulates the radioresistance of Non-Small cell lung
            cancer cells via NLRP3 Inflammasome-Mediated pyroptosis.
REFERENCE   2  (bases 1 to 4815)
  AUTHORS   Wu,G., Wang,D., Xiong,F., Liu,W., Wang,Q., Chen,J., Wang,B. and
            Chen,Y.
  TITLE     Upregulation of RSPO3 via targeted promoter DNA demethylation
            inhibits the progression of cholangiocarcinoma
  JOURNAL   Clin Epigenetics 15 (1), 177 (2023)
   PUBMED   37932819
  REMARK    GeneRIF: Upregulation of RSPO3 via targeted promoter DNA
            demethylation inhibits the progression of cholangiocarcinoma.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4815)
  AUTHORS   Ning,N., Wang,Q., Li,J., Liu,B., Chen,G., Hui,J. and An,L.
  TITLE     Amniotic fluid stem cell attenuated necrotizing enterocolitis
            progression by promoting Rspo3/AMPKalpha axis
  JOURNAL   Immunobiology 228 (3), 152336 (2023)
   PUBMED   37173190
  REMARK    GeneRIF: Amniotic fluid stem cell attenuated necrotizing
            enterocolitis progression by promoting Rspo3/AMPKalpha axis.
REFERENCE   4  (bases 1 to 4815)
  AUTHORS   Jeong,J.H., Yun,J.W., Kim,H.Y., Heo,C.Y. and Lee,S.
  TITLE     Investigation of cell signalings and therapeutic targets in
            PTPRK-RSPO3 fusion-positive colorectal cancer
  JOURNAL   PLoS One 17 (9), e0274555 (2022)
   PUBMED   36129915
  REMARK    GeneRIF: Investigation of cell signalings and therapeutic targets
            in PTPRK-RSPO3 fusion-positive colorectal cancer.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4815)
  AUTHORS   Takahashi,T., Li,Y., Chen,W., Nyasha,M.R., Ogawa,K., Suzuki,K.,
            Koide,M., Hagiwara,Y., Itoi,E., Aizawa,T., Tsuchiya,M., Suzuki,N.,
            Aoki,M. and Kanzaki,M.
  TITLE     RSPO3 is a novel contraction-inducible factor identified in an 'in
            vitro exercise model' using primary human myotubes
  JOURNAL   Sci Rep 12 (1), 14291 (2022)
   PUBMED   35995979
  REMARK    GeneRIF: RSPO3 is a novel contraction-inducible factor identified
            in an ''in vitro exercise model'' using primary human myotubes.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4815)
  AUTHORS   Heid,I.M., Jackson,A.U., Randall,J.C., Winkler,T.W., Qi,L.,
            Steinthorsdottir,V., Thorleifsson,G., Zillikens,M.C.,
            Speliotes,E.K., Magi,R., Workalemahu,T., White,C.C.,
            Bouatia-Naji,N., Harris,T.B., Berndt,S.I., Ingelsson,E.,
            Willer,C.J., Weedon,M.N., Luan,J., Vedantam,S., Esko,T.,
            Kilpelainen,T.O., Kutalik,Z., Li,S., Monda,K.L., Dixon,A.L.,
            Holmes,C.C., Kaplan,L.M., Liang,L., Min,J.L., Moffatt,M.F.,
            Molony,C., Nicholson,G., Schadt,E.E., Zondervan,K.T., Feitosa,M.F.,
            Ferreira,T., Lango Allen,H., Weyant,R.J., Wheeler,E., Wood,A.R.,
            Estrada,K., Goddard,M.E., Lettre,G., Mangino,M., Nyholt,D.R.,
            Purcell,S., Smith,A.V., Visscher,P.M., Yang,J., McCarroll,S.A.,
            Nemesh,J., Voight,B.F., Absher,D., Amin,N., Aspelund,T., Coin,L.,
            Glazer,N.L., Hayward,C., Heard-Costa,N.L., Hottenga,J.J.,
            Johansson,A., Johnson,T., Kaakinen,M., Kapur,K., Ketkar,S.,
            Knowles,J.W., Kraft,P., Kraja,A.T., Lamina,C., Leitzmann,M.F.,
            McKnight,B., Morris,A.P., Ong,K.K., Perry,J.R., Peters,M.J.,
            Polasek,O., Prokopenko,I., Rayner,N.W., Ripatti,S., Rivadeneira,F.,
            Robertson,N.R., Sanna,S., Sovio,U., Surakka,I., Teumer,A., van
            Wingerden,S., Vitart,V., Zhao,J.H., Cavalcanti-Proenca,C.,
            Chines,P.S., Fisher,E., Kulzer,J.R., Lecoeur,C., Narisu,N.,
            Sandholt,C., Scott,L.J., Silander,K., Stark,K., Tammesoo,M.L.,
            Teslovich,T.M., Timpson,N.J., Watanabe,R.M., Welch,R.,
            Chasman,D.I., Cooper,M.N., Jansson,J.O., Kettunen,J.,
            Lawrence,R.W., Pellikka,N., Perola,M., Vandenput,L., Alavere,H.,
            Almgren,P., Atwood,L.D., Bennett,A.J., Biffar,R., Bonnycastle,L.L.,
            Bornstein,S.R., Buchanan,T.A., Campbell,H., Day,I.N., Dei,M.,
            Dorr,M., Elliott,P., Erdos,M.R., Eriksson,J.G., Freimer,N.B.,
            Fu,M., Gaget,S., Geus,E.J., Gjesing,A.P., Grallert,H., Grassler,J.,
            Groves,C.J., Guiducci,C., Hartikainen,A.L., Hassanali,N.,
            Havulinna,A.S., Herzig,K.H., Hicks,A.A., Hui,J., Igl,W.,
            Jousilahti,P., Jula,A., Kajantie,E., Kinnunen,L., Kolcic,I.,
            Koskinen,S., Kovacs,P., Kroemer,H.K., Krzelj,V., Kuusisto,J.,
            Kvaloy,K., Laitinen,J., Lantieri,O., Lathrop,G.M., Lokki,M.L.,
            Luben,R.N., Ludwig,B., McArdle,W.L., McCarthy,A., Morken,M.A.,
            Nelis,M., Neville,M.J., Pare,G., Parker,A.N., Peden,J.F.,
            Pichler,I., Pietilainen,K.H., Platou,C.G., Pouta,A.,
            Ridderstrale,M., Samani,N.J., Saramies,J., Sinisalo,J., Smit,J.H.,
            Strawbridge,R.J., Stringham,H.M., Swift,A.J., Teder-Laving,M.,
            Thomson,B., Usala,G., van Meurs,J.B., van Ommen,G.J., Vatin,V.,
            Volpato,C.B., Wallaschofski,H., Walters,G.B., Widen,E., Wild,S.H.,
            Willemsen,G., Witte,D.R., Zgaga,L., Zitting,P., Beilby,J.P.,
            James,A.L., Kahonen,M., Lehtimaki,T., Nieminen,M.S., Ohlsson,C.,
            Palmer,L.J., Raitakari,O., Ridker,P.M., Stumvoll,M., Tonjes,A.,
            Viikari,J., Balkau,B., Ben-Shlomo,Y., Bergman,R.N., Boeing,H.,
            Smith,G.D., Ebrahim,S., Froguel,P., Hansen,T., Hengstenberg,C.,
            Hveem,K., Isomaa,B., Jorgensen,T., Karpe,F., Khaw,K.T., Laakso,M.,
            Lawlor,D.A., Marre,M., Meitinger,T., Metspalu,A., Midthjell,K.,
            Pedersen,O., Salomaa,V., Schwarz,P.E., Tuomi,T., Tuomilehto,J.,
            Valle,T.T., Wareham,N.J., Arnold,A.M., Beckmann,J.S., Bergmann,S.,
            Boerwinkle,E., Boomsma,D.I., Caulfield,M.J., Collins,F.S.,
            Eiriksdottir,G., Gudnason,V., Gyllensten,U., Hamsten,A.,
            Hattersley,A.T., Hofman,A., Hu,F.B., Illig,T., Iribarren,C.,
            Jarvelin,M.R., Kao,W.H., Kaprio,J., Launer,L.J., Munroe,P.B.,
            Oostra,B., Penninx,B.W., Pramstaller,P.P., Psaty,B.M.,
            Quertermous,T., Rissanen,A., Rudan,I., Shuldiner,A.R., Soranzo,N.,
            Spector,T.D., Syvanen,A.C., Uda,M., Uitterlinden,A., Volzke,H.,
            Vollenweider,P., Wilson,J.F., Witteman,J.C., Wright,A.F.,
            Abecasis,G.R., Boehnke,M., Borecki,I.B., Deloukas,P.,
            Frayling,T.M., Groop,L.C., Haritunians,T., Hunter,D.J.,
            Kaplan,R.C., North,K.E., O'Connell,J.R., Peltonen,L.,
            Schlessinger,D., Strachan,D.P., Hirschhorn,J.N., Assimes,T.L.,
            Wichmann,H.E., Thorsteinsdottir,U., van Duijn,C.M., Stefansson,K.,
            Cupples,L.A., Loos,R.J., Barroso,I., McCarthy,M.I., Fox,C.S.,
            Mohlke,K.L. and Lindgren,C.M.
  CONSRTM   MAGIC
  TITLE     Meta-analysis identifies 13 new loci associated with waist-hip
            ratio and reveals sexual dimorphism in the genetic basis of fat
            distribution
  JOURNAL   Nat Genet 42 (11), 949-960 (2010)
   PUBMED   20935629
  REMARK    GeneRIF: Observational study and genome-wide association study of
            gene-disease association. (HuGE Navigator)
            Erratum:[Nat Genet. 2011 Nov;43(11):1164]
REFERENCE   7  (bases 1 to 4815)
  AUTHORS   Vieira,A.R., McHenry,T.G., Daack-Hirsch,S., Murray,J.C. and
            Marazita,M.L.
  TITLE     Candidate gene/loci studies in cleft lip/palate and dental
            anomalies finds novel susceptibility genes for clefts
  JOURNAL   Genet Med 10 (9), 668-674 (2008)
   PUBMED   18978678
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   8  (bases 1 to 4815)
  AUTHORS   Kim,K.A., Zhao,J., Andarmani,S., Kakitani,M., Oshima,T.,
            Binnerts,M.E., Abo,A., Tomizuka,K. and Funk,W.D.
  TITLE     R-Spondin proteins: a novel link to beta-catenin activation
  JOURNAL   Cell Cycle 5 (1), 23-26 (2006)
   PUBMED   16357527
REFERENCE   9  (bases 1 to 4815)
  AUTHORS   Chen,J.Z., Wang,S., Tang,R., Yang,Q.S., Zhao,E., Chao,Y., Ying,K.,
            Xie,Y. and Mao,Y.M.
  TITLE     Cloning and identification of a cDNA that encodes a novel human
            protein with thrombospondin type I repeat domain, hPWTSR
  JOURNAL   Mol Biol Rep 29 (3), 287-292 (2002)
   PUBMED   12463421
  REMARK    GeneRIF: the gene is a novel member of thrombospondin type I repeat
            supergene family (hPWTSR)
REFERENCE   10 (bases 1 to 4815)
  AUTHORS   Adams,J.C. and Tucker,R.P.
  TITLE     The thrombospondin type 1 repeat (TSR) superfamily: diverse
            proteins with related roles in neuronal development
  JOURNAL   Dev Dyn 218 (2), 280-299 (2000)
   PUBMED   10842357
  REMARK    Review article
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL031776.7, AI095468.1,
            AF251057.1, AA568446.1 and AL590733.8.
            
            On Nov 23, 2018 this sequence version replaced NM_032784.4.
            
            Summary: This gene belongs to the R-spondin family. The encoded
            protein plays a role in the regulation of Wnt (wingless-type MMTV
            integration site family)/beta-catenin and Wnt/planar cell polarity
            (PCP) signaling pathways, which are involved in development, cell
            growth and disease pathogenesis. Genome-wide association studies
            suggest a correlation of this gene with bone mineral density and
            risk of fracture. This gene may be involved in tumor development.
            [provided by RefSeq, Jul 2013].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF251057.1, SRR1660805.165629.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1966682, SAMEA1968189
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000356698.9/ ENSP00000349131.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-232               AL031776.7         13350-13581         c
            233-240             AI095468.1         1-8
            241-2230            AF251057.1         1-1990
            2231-2373           AA568446.1         25-167              c
            2374-4815           AL590733.8         66789-69230
FEATURES             Location/Qualifiers
     source          1..4815
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q22.33"
     gene            1..4815
                     /gene="RSPO3"
                     /gene_synonym="CRISTIN1; PWTSR; THSD2"
                     /note="R-spondin 3"
                     /db_xref="GeneID:84870"
                     /db_xref="HGNC:HGNC:20866"
                     /db_xref="MIM:610574"
     exon            1..619
                     /gene="RSPO3"
                     /gene_synonym="CRISTIN1; PWTSR; THSD2"
                     /inference="alignment:Splign:2.1.0"
     CDS             523..1341
                     /gene="RSPO3"
                     /gene_synonym="CRISTIN1; PWTSR; THSD2"
                     /note="thrombospondin, type I, domain containing 2; roof
                     plate-specific spondin-3; protein with TSP type-1 repeat;
                     thrombospondin type-1 domain-containing protein 2;
                     R-spondin 3 homolog"
                     /codon_start=1
                     /product="R-spondin-3 precursor"
                     /protein_id="NP_116173.2"
                     /db_xref="CCDS:CCDS5135.1"
                     /db_xref="GeneID:84870"
                     /db_xref="HGNC:HGNC:20866"
                     /db_xref="MIM:610574"
                     /translation="MHLRLISWLFIILNFMEYIGSQNASRGRRQRRMHPNVSQGCQGG
                     CATCSDYNGCLSCKPRLFFALERIGMKQIGVCLSSCPSGYYGTRYPDINKCTKCKADC
                     DTCFNKNFCTKCKSGFYLHLGKCLDNCPEGLEANNHTMECVSIVHCEVSEWNPWSPCT
                     KKGKTCGFKRGTETRVREIIQHPSAKGNLCPPTNETRKCTVQRKKCQKGERGKKGRER
                     KRKKPNKGESKEAIPDSKSLESSKEIPEQRENKQQQKKRKVQDKQKSVSVSTVH"
     exon            620..811
                     /gene="RSPO3"
                     /gene_synonym="CRISTIN1; PWTSR; THSD2"
                     /inference="alignment:Splign:2.1.0"
     exon            812..958
                     /gene="RSPO3"
                     /gene_synonym="CRISTIN1; PWTSR; THSD2"
                     /inference="alignment:Splign:2.1.0"
     exon            959..1156
                     /gene="RSPO3"
                     /gene_synonym="CRISTIN1; PWTSR; THSD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1157..4815
                     /gene="RSPO3"
                     /gene_synonym="CRISTIN1; PWTSR; THSD2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2345..2350
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RSPO3"
                     /gene_synonym="CRISTIN1; PWTSR; THSD2"
                     /note="hexamer: AATAAA"
     regulatory      2349..2354
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RSPO3"
                     /gene_synonym="CRISTIN1; PWTSR; THSD2"
                     /note="hexamer: AATATA"
     polyA_site      2367
                     /gene="RSPO3"
                     /gene_synonym="CRISTIN1; PWTSR; THSD2"
                     /note="major polyA site"
     polyA_site      2373
                     /gene="RSPO3"
                     /gene_synonym="CRISTIN1; PWTSR; THSD2"
     regulatory      3079..3084
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RSPO3"
                     /gene_synonym="CRISTIN1; PWTSR; THSD2"
                     /note="hexamer: TATAAA"
     polyA_site      3100
                     /gene="RSPO3"
                     /gene_synonym="CRISTIN1; PWTSR; THSD2"
     regulatory      4787..4792
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RSPO3"
                     /gene_synonym="CRISTIN1; PWTSR; THSD2"
                     /note="hexamer: AATAAT"
     polyA_site      4815
                     /gene="RSPO3"
                     /gene_synonym="CRISTIN1; PWTSR; THSD2"
ORIGIN      
        1 actggagcgt ctcctgctcg cgcacgccag aagcagctcg gggtctctcc gccgcccctt
       61 ggccatggcc gctgtgccga cggcgcccgc tcccctgcgc tccaggggcc cccgccgctg
      121 cagccgcagc cgccgcagct tctgagccca aggggccgcc gctgcagccg ccgccgccgc
      181 cgctcgcccg cccggatccc gcctgcggca gttgccgcac aacatgctac ctgcggccgc
      241 cccggcggct cctggaaccc cggttcgcgg cgatgccagc caccccagcg aagccgccgc
      301 agttcagtgc ttggataatt tgaaagtaca atagttggtt tccctgtcca cccgccccac
      361 ttcgcttgcc atcacagcac gcctatcgga tgtgagagga gaagtcccgc tgctcgggca
      421 ctgtctatat acgcctaaca cctacatata ttttaaaaac attaaatata attaacaatc
      481 aaaagaaaga ggagaaagga agggaagcat tactgggtta ctatgcactt gcgactgatt
      541 tcttggcttt ttatcatttt gaactttatg gaatacatcg gcagccaaaa cgcctcccgg
      601 ggaaggcgcc agcgaagaat gcatcctaac gttagtcaag gctgccaagg aggctgtgca
      661 acatgctcag attacaatgg atgtttgtca tgtaagccca gactattttt tgctctggaa
      721 agaattggca tgaagcagat tggagtatgt ctctcttcat gtccaagtgg atattatgga
      781 actcgatatc cagatataaa taagtgtaca aaatgcaaag ctgactgtga tacctgtttc
      841 aacaaaaatt tctgcacaaa atgtaaaagt ggattttact tacaccttgg aaagtgcctt
      901 gacaattgcc cagaagggtt ggaagccaac aaccatacta tggagtgtgt cagtattgtg
      961 cactgtgagg tcagtgaatg gaatccttgg agtccatgca cgaagaaggg aaaaacatgt
     1021 ggcttcaaaa gagggactga aacacgggtc cgagaaataa tacagcatcc ttcagcaaag
     1081 ggtaacctgt gtcccccaac aaatgagaca agaaagtgta cagtgcaaag gaagaagtgt
     1141 cagaagggag aacgaggaaa aaaaggaagg gagaggaaaa gaaaaaaacc taataaagga
     1201 gaaagtaaag aagcaatacc tgacagcaaa agtctggaat ccagcaaaga aatcccagag
     1261 caacgagaaa acaaacagca gcagaagaag cgaaaagtcc aagataaaca gaaatcggta
     1321 tcagtcagca ctgtacacta gagggttcca tgagattatt gtagactcat gatgctgcta
     1381 tctcaaccag atgcccagga caggtgctct agccattagg accacaaatg gacatgtcag
     1441 ttattgctct gtctaaacaa cattcccagt agttgctata ttcttcatac aagcatagtt
     1501 aacaacaaag agccaaaaga tcaaagaagg gatactttca gatggttgtc ttgtgtgctt
     1561 ctctgcattt ttaaaagaca agacattctt gtacatatta tcaataggct ataagatgta
     1621 acaacgaaat gatgacatct ggagaagaaa catcttttcc ttataaaaat gtgttttcaa
     1681 gctgttgttt taagaagcaa aagatagttc tgcaaattca aagatacagt atcccttcaa
     1741 aacaaatagg agttcaggga agagaaacat ccttcaaagg acagtgttgt tttgaccggg
     1801 agatctagag agtgctcaga attagggcct ggcatttgga atcacaggat ttatcatcac
     1861 agaaacaact gttttaagat tagttccatc actctcatcc tgtattttta taagaaacac
     1921 aagagtgcat accagaattg aatataccat atgggattgg agaaagacaa atgtggaaga
     1981 aatcatagag ctggagacta cttttgtgct ttacaaaact gtgaaggatt gtggtcacct
     2041 ggaacaggtc tccaatctat gttagcacta tgtggctcag cctctgttac cccttggatt
     2101 atatatcaac ctgtaaacat gtgcctgtaa cttacttcca aaaacaaaat catacttatt
     2161 agaagaaaat tctgatttta tagaaaaaaa atagagcaag gagaatataa catgtttgca
     2221 aagtcatgtg ttttctttct caatgaggga aaaacaattt tattacctgc ttaatggtcc
     2281 acctggaact aaaagggata ctattttcta acaaggtata tctagtaggg gagaaagcca
     2341 ccacaataaa tatatttgtt aatagttttt caagttttgt tcactctgtt ttattgtttg
     2401 ttttattgag aaattcttac tcttagagac tcatgaatta agaaagagaa ttctgctaac
     2461 tcagagaacc tggttcctat gtaattcaga atatattaca tttctcagta atatttgttt
     2521 tttgaatcca cctttatctg agccaatgga gatttactta tagcgtatta ggagatattt
     2581 attccatttt cttattttaa tcaacattct aattatagac acatgggcct ccctagctga
     2641 tttcactgct cccccttcat tgcttagaaa tgggcatcat ttcttgtatg tcagatcccc
     2701 ctgcatcttc aacatttagt cttttcttct ccatattttc tatctgtgga tctctttagg
     2761 ggattgaagt caccctagct gaaggcctca ccagtgtttc acagaggaca cagcccaccc
     2821 cttgcaggag gaggtatctc tgagtgtgca gcacagaatc gcatgaccca ccttaacctt
     2881 cctgttgtca tggaaggatg cacggctgct ctgtccactg tgattcctag ccctctcaag
     2941 atcactgctt tctgaagaat ttgcaatgac tctggcttct ggctgcttat ctctggacac
     3001 ccgttctcca ccagttgtac agttcatgta atctacttgg cttaattgat tttccacttc
     3061 tctcttcctc ttctaagata taaacatttt aaatgattta ttcctgtttc ttattctggt
     3121 gtttctttcc ttgtccctat gagataagtg tctcaactca ctaaatctat tcccaatgta
     3181 taaaataatt ctaattccat tttcagctaa aacatatatt accaagaaga aacaaacttt
     3241 atcctacaga atgatgttag gtagaaatat gtccccaggt ttgagacctt tcggatgatt
     3301 tcatatacca tctttcttct gagtgttacc cagtcaagta taagtagcca aattattttt
     3361 gcacatcttt ctgtttctca tgtcttcatt tattcaacaa gcacttactg ggaaggtcta
     3421 cacctgcata ggcaatgctg gaaaaagggt taagtaaacc aggacatgac aatggtggca
     3481 aatgactatc aggtcttccc atgtgtttga ctcaaactta ttaccctatg gtccttctga
     3541 caatggcaga aggtctgaat ccttgatgct aaacttatat aaaagtagaa ttattacaaa
     3601 ggaaaaagaa ataaaaacta acattcattt tcatatgttg gatgaaatat aaatgaagaa
     3661 aaagataaca tcaattttaa ctgtaattct ccatccacca gtaacagatc cttaagacaa
     3721 tagaatcata cagtattcaa accagcagcc ttctcaaatt tgagcaaaaa ctctatcaac
     3781 ctctggtaaa gttcctacac tagtcacaga aggtgttaac tttctactct gattctgtct
     3841 ccataatggg gtaaactgtt gatagtttac cccatcaaca gatggtcggt aaattattga
     3901 ttcgaagaat cgagagagtg cagcaacata aatctgttaa tgtctgatca agctcctgcc
     3961 ctgttctccg aattcagctt cataattaag ggaaggcctg ttttctatcc tcagatttag
     4021 gttctagtag cagttgtgta accactagtg agtcacttaa ctcctctggg tccccatttc
     4081 tcatgtgcaa caagaaagag gggaactgga gatgatcact ctagttccag acaagggaac
     4141 atttcacact ttgtttactt cagggtgatg tccctgagtc ctcattagtg actgcgtcct
     4201 ttggaagtta tcccaaccct gcttttctca aaagtgaaaa tgtataggct ctcagaggag
     4261 acagatttaa ctctgcttct ctaatgttat tgaattaaaa gctgttcaca ttagtggtta
     4321 ttaaatattg aaataacact gggaagaaaa agcatatata aatacagcta aaaacaagaa
     4381 tagatattca ttctcacaaa gggagacagc aaagaaaatg gaaagtgcac tggtgctagc
     4441 gttagacagc ttgtgttaat gtctcaattc tgctactaac tggttgcagc ttgtgtgacc
     4501 ttgggcacat tgtatgatct cgcagaatat catcccaaat ctgcaaaatg gaattggcat
     4561 catctctttt gcaagattgt tatgagaatt aaaaggttct tcattcaata taataataaa
     4621 tattttgtat ataaatgaat atcaattaaa agttatgact aattccacaa gtcaaacata
     4681 taaattttat ttcttgattc atgatatgtg atagtattca taaaaatgta catgcatgat
     4741 aatttcaagg aataagtata tatgtgagaa tcatggaaat gaaattaata atattaacta
     4801 gtaattaaat tgtaa
//
