LOCUS       NM_031924               6576 bp    mRNA    linear   PRI 12-OCT-2024
DEFINITION  Homo sapiens radial spoke head 3 (RSPH3), transcript variant 1,
            mRNA.
ACCESSION   NM_031924
VERSION     NM_031924.8
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6576)
  AUTHORS   Li,Y., Zhang,Q., Tan,Q., Sha,X., Gao,Y., Hua,R., Zhou,P., Wei,Z.,
            He,X., Cao,Y., Li,T. and Wu,H.
  TITLE     LRRC23 deficiency causes male infertility with idiopathic
            asthenozoospermia by disrupting the assembly of radial spokes
  JOURNAL   Clin Genet 104 (6), 694-699 (2023)
   PUBMED   37804054
REFERENCE   2  (bases 1 to 6576)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 6576)
  AUTHORS   Wu,H., Wang,J., Cheng,H., Gao,Y., Liu,W., Zhang,Z., Jiang,H.,
            Li,W., Zhu,F., Lv,M., Liu,C., Tan,Q., Zhang,X., Wang,C., Ni,X.,
            Chen,Y., Song,B., Zhou,P., Wei,Z., Zhang,F., He,X. and Cao,Y.
  TITLE     Patients with severe asthenoteratospermia carrying SPAG6 or RSPH3
            mutations have a positive pregnancy outcome following
            intracytoplasmic sperm injection
  JOURNAL   J Assist Reprod Genet 37 (4), 829-840 (2020)
   PUBMED   32124190
  REMARK    GeneRIF: Patients with severe asthenoteratospermia carrying SPAG6
            or RSPH3 mutations have a positive pregnancy outcome following
            intracytoplasmic sperm injection.
REFERENCE   4  (bases 1 to 6576)
  AUTHORS   Fragoza,R., Das,J., Wierbowski,S.D., Liang,J., Tran,T.N., Liang,S.,
            Beltran,J.F., Rivera-Erick,C.A., Ye,K., Wang,T.Y., Yao,L., Mort,M.,
            Stenson,P.D., Cooper,D.N., Wei,X., Keinan,A., Schimenti,J.C.,
            Clark,A.G. and Yu,H.
  TITLE     Extensive disruption of protein interactions by genetic variants
            across the allele frequency spectrum in human populations
  JOURNAL   Nat Commun 10 (1), 4141 (2019)
   PUBMED   31515488
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6576)
  AUTHORS   Jeanson,L., Thomas,L., Copin,B., Coste,A., Sermet-Gaudelus,I.,
            Dastot-Le Moal,F., Duquesnoy,P., Montantin,G., Collot,N.,
            Tissier,S., Papon,J.F., Clement,A., Louis,B., Escudier,E.,
            Amselem,S. and Legendre,M.
  TITLE     Mutations in GAS8, a Gene Encoding a Nexin-Dynein Regulatory
            Complex Subunit, Cause Primary Ciliary Dyskinesia with Axonemal
            Disorganization
  JOURNAL   Hum Mutat 37 (8), 776-785 (2016)
   PUBMED   27120127
REFERENCE   6  (bases 1 to 6576)
  AUTHORS   Jeanson,L., Copin,B., Papon,J.F., Dastot-Le Moal,F., Duquesnoy,P.,
            Montantin,G., Cadranel,J., Corvol,H., Coste,A., Desir,J.,
            Souayah,A., Kott,E., Collot,N., Tissier,S., Louis,B., Tamalet,A.,
            de Blic,J., Clement,A., Escudier,E., Amselem,S. and Legendre,M.
  TITLE     RSPH3 Mutations Cause Primary Ciliary Dyskinesia with
            Central-Complex Defects and a Near Absence of Radial Spokes
  JOURNAL   Am J Hum Genet 97 (1), 153-162 (2015)
   PUBMED   26073779
  REMARK    GeneRIF: RSPH3 Mutations Cause Primary Ciliary Dyskinesia with
            Central-Complex Defects and a Near Absence of Radial Spokes.
REFERENCE   7  (bases 1 to 6576)
  AUTHORS   Figueroa,J.D., Han,S.S., Garcia-Closas,M., Baris,D., Jacobs,E.J.,
            Kogevinas,M., Schwenn,M., Malats,N., Johnson,A., Purdue,M.P.,
            Caporaso,N., Landi,M.T., Prokunina-Olsson,L., Wang,Z.,
            Hutchinson,A., Burdette,L., Wheeler,W., Vineis,P., Siddiq,A.,
            Cortessis,V.K., Kooperberg,C., Cussenot,O., Benhamou,S.,
            Prescott,J., Porru,S., Bueno-de-Mesquita,H.B., Trichopoulos,D.,
            Ljungberg,B., Clavel-Chapelon,F., Weiderpass,E., Krogh,V.,
            Dorronsoro,M., Travis,R., Tjonneland,A., Brenan,P.,
            Chang-Claude,J., Riboli,E., Conti,D., Gago-Dominguez,M.,
            Stern,M.C., Pike,M.C., Van Den Berg,D., Yuan,J.M., Hohensee,C.,
            Rodabough,R., Cancel-Tassin,G., Roupret,M., Comperat,E., Chen,C.,
            De Vivo,I., Giovannucci,E., Hunter,D.J., Kraft,P., Lindstrom,S.,
            Carta,A., Pavanello,S., Arici,C., Mastrangelo,G., Karagas,M.R.,
            Schned,A., Armenti,K.R., Hosain,G.M., Haiman,C.A., Fraumeni,J.F.
            Jr., Chanock,S.J., Chatterjee,N., Rothman,N. and Silverman,D.T.
  TITLE     Genome-wide interaction study of smoking and bladder cancer risk
  JOURNAL   Carcinogenesis 35 (8), 1737-1744 (2014)
   PUBMED   24662972
REFERENCE   8  (bases 1 to 6576)
  AUTHORS   Jivan,A., Earnest,S., Juang,Y.C. and Cobb,M.H.
  TITLE     Radial spoke protein 3 is a mammalian protein kinase A-anchoring
            protein that binds ERK1/2
  JOURNAL   J Biol Chem 284 (43), 29437-29445 (2009)
   PUBMED   19684019
  REMARK    GeneRIF: Radial spoke protein 3 is a mammalian protein kinase
            A-anchoring protein that binds ERK1/2.
REFERENCE   9  (bases 1 to 6576)
  AUTHORS   Koukoulas,I., Augustine,C., Silkenbeumer,N., Gunnersen,J.M.,
            Scott,H.S. and Tan,S.S.
  TITLE     Genomic organisation and nervous system expression of radial spoke
            protein 3
  JOURNAL   Gene 336 (1), 15-23 (2004)
   PUBMED   15225872
REFERENCE   10 (bases 1 to 6576)
  AUTHORS   Zariwala,M.A., Knowles,M.R. and Leigh,M.W.
  TITLE     Primary Ciliary Dyskinesia
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301301
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL035530.11 and AF353618.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Mar 20, 2020 this sequence version replaced NM_031924.7.
            
            Summary: The protein encoded by this gene acts as a protein kinase
            A anchoring protein. Mutations in this gene cause primary ciliary
            dyskinesia; a disorder characterized by defects of the axoneme in
            motile cilia and sperm flagella. The homolog of this gene was first
            identified in the blue-green algae Chlamydomonas as encoding a
            radial spoke protein that formed a structural component of motile
            cilia and flagella. Alternate splicing results in multiple
            transcript variants encoding distinct isoforms. [provided by
            RefSeq, Dec 2016].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longer isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC050604.1, AK057931.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            MANE Ensembl match               :: ENST00000367069.7/
                                                ENSP00000356036.1
            RefSeq Select criteria           :: based on manual assertion,
                                                conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-437               AL035530.11        72587-73023
            438-2074            AF353618.1         1-1637
            2075-6576           AL035530.11        95417-99918
FEATURES             Location/Qualifiers
     source          1..6576
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q25.3"
     gene            1..6576
                     /gene="RSPH3"
                     /gene_synonym="CILD32; dJ111C20.1; RSHL2; RSP3"
                     /note="radial spoke head 3"
                     /db_xref="GeneID:83861"
                     /db_xref="HGNC:HGNC:21054"
                     /db_xref="MIM:615876"
     exon            1..768
                     /gene="RSPH3"
                     /gene_synonym="CILD32; dJ111C20.1; RSHL2; RSP3"
                     /inference="alignment:Splign:2.1.0"
     CDS             653..1909
                     /gene="RSPH3"
                     /gene_synonym="CILD32; dJ111C20.1; RSHL2; RSP3"
                     /note="isoform 1 is encoded by transcript variant 1;
                     radial spoke head protein 3 homolog; radial spoke
                     head-like protein 2; radial spoke 3 homolog; A-kinase
                     anchor protein RSPH3; radial spoke head 3 homolog"
                     /codon_start=1
                     /product="radial spoke head protein 3 homolog isoform 1"
                     /protein_id="NP_114130.4"
                     /db_xref="CCDS:CCDS5260.2"
                     /db_xref="GeneID:83861"
                     /db_xref="HGNC:HGNC:21054"
                     /db_xref="MIM:615876"
                     /translation="MASALTDRTSRAPSTYTYTSRPRALPCQRSRYRDSLTQPDEEPM
                     HYGNIMYDRRVIRGNTYALQTGPLLGRPDSLELQRQREARKRALARKQAQEQLRPQTP
                     EPVEGRKHVDVQTELYLEEIADRIIEVDMECQTDAFLDRPPTPLFIPAKTGKDVATQI
                     LEGELFDFDLEVKPVLEVLVGKTIEQSLLEVMEEEELANLRASQREYEELRNSERAEV
                     QRLEEQERRHREEKERRKKQQWEIMHKHNETSQKIAARAFAQRYLADLLPSVFGSLRD
                     SGYFYDPIERDIEIGFLPWLMNEVEKTMEYSMVGRTVLDMLIREVVEKRLCMYEHGED
                     THQSPEPEDEPGGPGAMTESLEASEFLEQSMSQTRELLLDGGYLQRTTYDRRSSQERK
                     FMEERELLGQDEETAMRKSLGEEELS"
     exon            769..856
                     /gene="RSPH3"
                     /gene_synonym="CILD32; dJ111C20.1; RSHL2; RSP3"
                     /inference="alignment:Splign:2.1.0"
     exon            857..998
                     /gene="RSPH3"
                     /gene_synonym="CILD32; dJ111C20.1; RSHL2; RSP3"
                     /inference="alignment:Splign:2.1.0"
     exon            999..1144
                     /gene="RSPH3"
                     /gene_synonym="CILD32; dJ111C20.1; RSHL2; RSP3"
                     /inference="alignment:Splign:2.1.0"
     exon            1145..1348
                     /gene="RSPH3"
                     /gene_synonym="CILD32; dJ111C20.1; RSHL2; RSP3"
                     /inference="alignment:Splign:2.1.0"
     exon            1349..1511
                     /gene="RSPH3"
                     /gene_synonym="CILD32; dJ111C20.1; RSHL2; RSP3"
                     /inference="alignment:Splign:2.1.0"
     exon            1512..1598
                     /gene="RSPH3"
                     /gene_synonym="CILD32; dJ111C20.1; RSHL2; RSP3"
                     /inference="alignment:Splign:2.1.0"
     exon            1599..6576
                     /gene="RSPH3"
                     /gene_synonym="CILD32; dJ111C20.1; RSHL2; RSP3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2056..2061
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RSPH3"
                     /gene_synonym="CILD32; dJ111C20.1; RSHL2; RSP3"
                     /note="hexamer: GATAAA"
     polyA_site      2079
                     /gene="RSPH3"
                     /gene_synonym="CILD32; dJ111C20.1; RSHL2; RSP3"
     regulatory      6555..6560
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RSPH3"
                     /gene_synonym="CILD32; dJ111C20.1; RSHL2; RSP3"
                     /note="hexamer: AATAAA"
     polyA_site      6576
                     /gene="RSPH3"
                     /gene_synonym="CILD32; dJ111C20.1; RSHL2; RSP3"
                     /note="major polyA site"
ORIGIN      
        1 cttccccgcc cgggcacgta acagagctgg gagcctggct gccaggggcg tgttatcgcg
       61 ggggcagcct gggagcgctg ctgcgccgcg gcaggagaaa cagccgagga caccctggaa
      121 gagcaggagc acgtgggcct gcgagaatgg cgccaacacc agccctgcaa agccacattc
      181 caaagccggg ccaaggacag ccaccgttgc cccgcaggcc tcaaggatga cggtcaagcc
      241 agccaaggcc gcctccctag ccaggaacct cgcaaagcgc aggcgcacct acctgggtgg
      301 cgcggctggg cggagtcaag agccggaagt cccttgtgcc gcagtccttc ccgggaaacc
      361 tggggaccgg aactgcccgg agttcccgcc cccagacaga accctgggtt gctgggcaac
      421 cgatgcggcc cccgcagctg gtctctgtgg tgctgggagc gagcccagta tagctcccac
      481 ctcctgcgct ggtaacctcc cgtcccgccc gcctcccttg ttgtcgcctc tcctcgcgtc
      541 gcggaatcct tgcccttggc actacttaca tctctccggg tcccacaaca ccttagcgcc
      601 cacctgcttc aaagccaagc tccaccgaaa gcgaggcagt cagcccccgg acatggcctc
      661 agcgctgact gatcgcacct ctcgggcccc gagcacctac acctacacca gccggccccg
      721 agcactgccc tgccagcgca gccgttaccg ggacagcctg acgcagccag atgaagaacc
      781 tatgcattat ggaaacataa tgtatgacag aagggtaatt cgaggtaaca cttatgcact
      841 ccagacaggg ccactgctcg gacggcctga ttctctagag ctccagagac aacgggaggc
      901 taggaagagg gctcttgcca gaaaacaagc ccaagagcag ctcagaccac aaacacctga
      961 acctgtggaa ggcagaaagc atgtcgatgt gcaaacagaa ttataccttg aagaaattgc
     1021 tgatcgcata atagaagttg atatggaatg ccaaacagat gcatttttgg acagaccacc
     1081 aacaccactc tttattcctg ccaaaactgg caaagatgtg gccacccaaa tactagaagg
     1141 agagctcttt gactttgatc ttgaagttaa accagtgtta gaagttttgg tggggaagac
     1201 aattgagcag tctcttctgg aagtaatgga agaagaagag ctggctaacc tgcgggccag
     1261 tcagcgtgag tatgaagaac tacggaatag tgaacgtgct gaagttcaac gacttgaaga
     1321 gcaagagagg cgacaccgag aagaaaaaga acggcgtaag aaacagcagt gggaaataat
     1381 gcacaagcac aacgagacat cacaaaaaat cgccgcccga gcatttgcac agcgttacct
     1441 ggctgacctt ctcccgtctg tttttggcag cctcagggat agtggctact tttatgatcc
     1501 cattgaaaga gatattgaga taggatttct tccatggcta atgaatgaag ttgaaaaaac
     1561 catggaatat agcatggtgg gaagaacagt gcttgacatg ttgatccgtg aggtggttga
     1621 aaagaggctg tgtatgtatg agcatgggga agacacacat cagtctccag aacccgagga
     1681 tgagcctggt ggtcctggag caatgacaga gtcactggag gcctctgaat tcctggagca
     1741 gagcatgtca cagacacggg agctgctttt agatggaggc tacctacaaa gaacaacata
     1801 tgacagaagg tcatcccagg aaaggaagtt tatggaagag agagaactct tagggcaaga
     1861 tgaagaaaca gcaatgagga agtccttagg ggaggaagaa ttgtcatagg agggaagctt
     1921 caaagtcatg aaaccaatca acagccaagt cagcaagcaa tcaggtaatg atgtgcgagt
     1981 cccctcaggt tatagaaatt atgttgtatt tagtcagtat gtctttttgt gtaatggact
     2041 gggccaatca aatgtgataa aattaatttg agaatgaaac ttaaattgaa ttcatttatt
     2101 taatagaata ttcaaaggct atattgtaat tctaatgaga attatattta attactagct
     2161 ttctattacc attatatgta accagcaaat atatttttcc ttagcccttc taattttgcg
     2221 ggttagtttt ttttagtaag tatgatattt aatgttcaat atcagtgctg tcagccatat
     2281 agcttttctt ctctatagga aaactatttc acctactgta agagtgaatg cctagccagg
     2341 cgtggtggct cacacctgta atcccagcac tttgggaggc tgaggctggc agatcccctg
     2401 aggtcgggag tttgagacca gccagaccaa caaggagaaa ccccatctct gctaaaaata
     2461 caaaattagc caggcatggt ggcgcatgcc tgtaatccta gctacttggg aggctgaggc
     2521 aggagaatca cttgaacctg gggcggaggt tgcggtgagc caagtttgca ccattgcact
     2581 ccagcctggg caacaagagc aaaactccgt ctcaaaaaaa aaagaaaaaa aagagagaga
     2641 atgcctaaac gttgtgctac tgaaagccac agtgctccct tcctgcaagg accatcttca
     2701 atattcaagt aaggcttaag gaattcagtg gattagtata tgctctcatc tattttttca
     2761 cattcctact atgaaaaaat ttcaggcaat gccaataaag caaaagtccc tgtttgccct
     2821 catacagtct cactcccctc ctcaaaggta gatcactgtt atcaggtatg agctatctag
     2881 gccttttttg aggtattcct atacaaatgt atttgaaaat ctagaaatat atagcttgat
     2941 tttctttata taaatataaa cattatttac atattttatt tttcacttaa gagaatgtct
     3001 tagatctttc aaggttagtg gcatcaatct cttatcttgt caaaatatgg ttaaggtatt
     3061 acatattatg actgtgctgt agcataggga attaacattt gactttttaa tctgaatgtg
     3121 ttagagaatt tacctgcagt attaaaatca caagttattt tctctccaaa gccttcctaa
     3181 tcgtaagcaa taactgaagt cttcagagcc tttagataaa tagagcctat ttttcttttt
     3241 acttattttt atttttattt tttggagaca aggtctcact ctgacaccca ggctggagtg
     3301 cagcagcacc atcacagctc actgcagcct tgacctccca ggctcaggtg atcttctcac
     3361 ctcagccttc caagtagctg ggactacagg tgtgcaccac cacgtctggc tagttactgt
     3421 attttttgta gagatgggct cttgctgtgt tgcccaggct ggtctccaac tcctgggctc
     3481 aagtgatctg cccaccttgg cctcccaaag tgctaggact acaggcatga gccaccgcac
     3541 ctggctgagt ctgtttttcg caaaatactt acacatatat aaacgtagtg atttaaaata
     3601 acttataatt attaacccat ttaccttaat ttcttttaag ttaggtacca agaatgtatc
     3661 cacatttaaa aaattttaaa agaatgcacc cattttccca tatagtattt cactataaca
     3721 cttaggcctc atggcaacct tctgaagaat gcatgacaat atttgtattt tttagtttct
     3781 aatccttaat tcggtcagcc agcaaacatt agctgagtgc ctgctctgcc ttgggaatga
     3841 gactgcagat acagcggtga acagtcacga atactacatg ttttttacag cctagcagtg
     3901 gagctaaatg aagtaaatga tgtaaataaa gaaaccaaag ctcaggaatt taaagtgatt
     3961 tgcaatagat gacgagccta gtagggaata ggactaaagc tggaatccaa atctccttac
     4021 ttctaatctg gtatactttc ttctaataac ttgctgtttt tgtgaggtac aaagaataat
     4081 ttaaaataaa ttattgcact aatagcattt taaaaattgg aatcacgcct cttatccatc
     4141 atggggctgg ttccatcttg ttaaacagtc tttatgatca tcctaacatt tataagatat
     4201 taattttggc aagaagacca aaaattttta attagggcta ttgctgatta ttattgttat
     4261 taccaatttt aaaatatcta caactagctt ttagaaacct taaaattggg ccaggcacgg
     4321 tggcttgcac ctgtaatccc agcactttgg gaggccgagg caggcggatc acaaggtcag
     4381 gagatcaaga ccatcctggc caacatggtg aaaccccgtc tctactaaaa atacaaaaat
     4441 tagtcaggcg tggtggcggg cacctgtagt cccagctact caggaggccg aggcaggaga
     4501 atcgcttgaa cacgggaggc ggaggttgca gtgagccgag atcgcgccac tgcactccag
     4561 cctggaagac agagtgagac tccttctcaa aaaaaaaaaa ccttaaaatt gatttgaagt
     4621 tgattatctt tcataatctt ctaattctcc tttagaatga tccagtccat taaacacttt
     4681 gcagatgcca gacctatgct ctataaatcc ataaattaat tcaatttatt gaaagaagta
     4741 gatatctata aaccccacac tgaactggtt aagcttaatt gcaatcctag tccatcctag
     4801 agagaaatca aaataattga gaggttaggg cattgtcgct gtgagaaagc ttagaggtaa
     4861 cacattatat gttatctgga gagaagaatg ttaaatggaa acaaagttta tgaagaattt
     4921 cttcaagtac ctgaaggact ttctgggaaa ttacaagata gcattcttag gttccatatg
     4981 tctagaagac ttttcataac tgtaagcaac ttttgcaaat ggtttgggaa cataaaactc
     5041 ttccagcata gaagaaaatg ggtactatta acattcagat ggttatggga ccatcataac
     5101 agtttgatgt gatatgcaaa atgctacatc cccaactatg accctcagta tgacttttaa
     5161 aggaattata agctccaaat actttttgga gtataaacag tacttgactt tttcacacat
     5221 ttgctaatac ccttctcata agttttattt ctttctgatg tttcagctaa cactgagtta
     5281 acagaagcta aataataata aaatgaagaa cagatagtaa atatattgaa attgtcattc
     5341 ctggccgggt gtggtggctt acgcctataa tcccagcact ttgggaggcc aaggcaggca
     5401 gatcacgagg tcaggagatc gaggccatcc tggccaacat ggtgaaaccc tgtctccact
     5461 aaaaatacaa aaattagctg ggtatggtga cgcatgccta taatcccagc tactcgggag
     5521 actgaggcat aagaatcact tgaacccagg aggtcgcgcc gctgcactcc agcccggtga
     5581 cagagcaaga ctccgtctca aaaaaaaaaa aaagaaagaa aaagaaattg tcattcttaa
     5641 cataaattcc catatcacta aaatagatca gagaggtaga atgaaatttt ctctagcata
     5701 ggcatcatta catttaagcc atctgagggc tatgagcgtg gcctgcaaag tcactctgag
     5761 caacacaagg tggagtttga aagcagattc aaagccagac cactgggctt gagtcctcac
     5821 atgcaagcat gtagtcttca ccatggagaa gggggtttgc ctttctcaaa gcttttttct
     5881 tttaaattaa gaaaaagaac tgatttatca gtctagtgtt tcattttaaa aacatatgaa
     5941 agggtgggta acttgcagaa gaaagtattt ggagcctata attccattaa aagttataat
     6001 ggcggtcata gttggggaag cagcattttg catattatat caaaatgtta tggtaccata
     6061 accatccaaa tgttaatggt accaatttct tctatcctag aaacatttca ttttatgttg
     6121 ttgaaacata acaactatat cagctagatt ttttttctat gctttacctg ctatggaaaa
     6181 tttgacacat tctgctttac tcttttgttt ataggtgaat cacagaatgt atttttatgt
     6241 attctgtagt tcaatagcca tggctgttta cttcatttaa tttatttagc ataaagacat
     6301 tatgaaaagg cctaaacatg agcttcactt ccccactaac taattagcat ctgttatttc
     6361 ttaaccgtaa tgcctaggcc taacctcaac caaccttcca caatcttgtt cacagtcaga
     6421 gacccaaaaa agagtgttat aagtggggtg tcactattgt gaaagtagtg ctaatggtgt
     6481 tgaattggta cctatgattc ttcttttgaa ttagattgta tgtttcatga tctcaactta
     6541 cattactaaa attcaataaa ctgatcattt agatga
//
