LOCUS       NM_001346418            6283 bp    mRNA    linear   PRI 06-OCT-2024
DEFINITION  Homo sapiens radial spoke head 3 (RSPH3), transcript variant 2,
            mRNA.
ACCESSION   NM_001346418 XM_005267153
VERSION     NM_001346418.1
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6283)
  AUTHORS   Li,Y., Zhang,Q., Tan,Q., Sha,X., Gao,Y., Hua,R., Zhou,P., Wei,Z.,
            He,X., Cao,Y., Li,T. and Wu,H.
  TITLE     LRRC23 deficiency causes male infertility with idiopathic
            asthenozoospermia by disrupting the assembly of radial spokes
  JOURNAL   Clin Genet 104 (6), 694-699 (2023)
   PUBMED   37804054
REFERENCE   2  (bases 1 to 6283)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 6283)
  AUTHORS   Wu,H., Wang,J., Cheng,H., Gao,Y., Liu,W., Zhang,Z., Jiang,H.,
            Li,W., Zhu,F., Lv,M., Liu,C., Tan,Q., Zhang,X., Wang,C., Ni,X.,
            Chen,Y., Song,B., Zhou,P., Wei,Z., Zhang,F., He,X. and Cao,Y.
  TITLE     Patients with severe asthenoteratospermia carrying SPAG6 or RSPH3
            mutations have a positive pregnancy outcome following
            intracytoplasmic sperm injection
  JOURNAL   J Assist Reprod Genet 37 (4), 829-840 (2020)
   PUBMED   32124190
  REMARK    GeneRIF: Patients with severe asthenoteratospermia carrying SPAG6
            or RSPH3 mutations have a positive pregnancy outcome following
            intracytoplasmic sperm injection.
REFERENCE   4  (bases 1 to 6283)
  AUTHORS   Fragoza,R., Das,J., Wierbowski,S.D., Liang,J., Tran,T.N., Liang,S.,
            Beltran,J.F., Rivera-Erick,C.A., Ye,K., Wang,T.Y., Yao,L., Mort,M.,
            Stenson,P.D., Cooper,D.N., Wei,X., Keinan,A., Schimenti,J.C.,
            Clark,A.G. and Yu,H.
  TITLE     Extensive disruption of protein interactions by genetic variants
            across the allele frequency spectrum in human populations
  JOURNAL   Nat Commun 10 (1), 4141 (2019)
   PUBMED   31515488
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6283)
  AUTHORS   Jeanson,L., Thomas,L., Copin,B., Coste,A., Sermet-Gaudelus,I.,
            Dastot-Le Moal,F., Duquesnoy,P., Montantin,G., Collot,N.,
            Tissier,S., Papon,J.F., Clement,A., Louis,B., Escudier,E.,
            Amselem,S. and Legendre,M.
  TITLE     Mutations in GAS8, a Gene Encoding a Nexin-Dynein Regulatory
            Complex Subunit, Cause Primary Ciliary Dyskinesia with Axonemal
            Disorganization
  JOURNAL   Hum Mutat 37 (8), 776-785 (2016)
   PUBMED   27120127
REFERENCE   6  (bases 1 to 6283)
  AUTHORS   Jeanson,L., Copin,B., Papon,J.F., Dastot-Le Moal,F., Duquesnoy,P.,
            Montantin,G., Cadranel,J., Corvol,H., Coste,A., Desir,J.,
            Souayah,A., Kott,E., Collot,N., Tissier,S., Louis,B., Tamalet,A.,
            de Blic,J., Clement,A., Escudier,E., Amselem,S. and Legendre,M.
  TITLE     RSPH3 Mutations Cause Primary Ciliary Dyskinesia with
            Central-Complex Defects and a Near Absence of Radial Spokes
  JOURNAL   Am J Hum Genet 97 (1), 153-162 (2015)
   PUBMED   26073779
  REMARK    GeneRIF: RSPH3 Mutations Cause Primary Ciliary Dyskinesia with
            Central-Complex Defects and a Near Absence of Radial Spokes.
REFERENCE   7  (bases 1 to 6283)
  AUTHORS   Figueroa,J.D., Han,S.S., Garcia-Closas,M., Baris,D., Jacobs,E.J.,
            Kogevinas,M., Schwenn,M., Malats,N., Johnson,A., Purdue,M.P.,
            Caporaso,N., Landi,M.T., Prokunina-Olsson,L., Wang,Z.,
            Hutchinson,A., Burdette,L., Wheeler,W., Vineis,P., Siddiq,A.,
            Cortessis,V.K., Kooperberg,C., Cussenot,O., Benhamou,S.,
            Prescott,J., Porru,S., Bueno-de-Mesquita,H.B., Trichopoulos,D.,
            Ljungberg,B., Clavel-Chapelon,F., Weiderpass,E., Krogh,V.,
            Dorronsoro,M., Travis,R., Tjonneland,A., Brenan,P.,
            Chang-Claude,J., Riboli,E., Conti,D., Gago-Dominguez,M.,
            Stern,M.C., Pike,M.C., Van Den Berg,D., Yuan,J.M., Hohensee,C.,
            Rodabough,R., Cancel-Tassin,G., Roupret,M., Comperat,E., Chen,C.,
            De Vivo,I., Giovannucci,E., Hunter,D.J., Kraft,P., Lindstrom,S.,
            Carta,A., Pavanello,S., Arici,C., Mastrangelo,G., Karagas,M.R.,
            Schned,A., Armenti,K.R., Hosain,G.M., Haiman,C.A., Fraumeni,J.F.
            Jr., Chanock,S.J., Chatterjee,N., Rothman,N. and Silverman,D.T.
  TITLE     Genome-wide interaction study of smoking and bladder cancer risk
  JOURNAL   Carcinogenesis 35 (8), 1737-1744 (2014)
   PUBMED   24662972
REFERENCE   8  (bases 1 to 6283)
  AUTHORS   Jivan,A., Earnest,S., Juang,Y.C. and Cobb,M.H.
  TITLE     Radial spoke protein 3 is a mammalian protein kinase A-anchoring
            protein that binds ERK1/2
  JOURNAL   J Biol Chem 284 (43), 29437-29445 (2009)
   PUBMED   19684019
  REMARK    GeneRIF: Radial spoke protein 3 is a mammalian protein kinase
            A-anchoring protein that binds ERK1/2.
REFERENCE   9  (bases 1 to 6283)
  AUTHORS   Koukoulas,I., Augustine,C., Silkenbeumer,N., Gunnersen,J.M.,
            Scott,H.S. and Tan,S.S.
  TITLE     Genomic organisation and nervous system expression of radial spoke
            protein 3
  JOURNAL   Gene 336 (1), 15-23 (2004)
   PUBMED   15225872
REFERENCE   10 (bases 1 to 6283)
  AUTHORS   Zariwala,M.A., Knowles,M.R. and Leigh,M.W.
  TITLE     Primary Ciliary Dyskinesia
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301301
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK057931.1, AK057655.1,
            AL035530.11 and AI028007.1.
            
            On Oct 9, 2016 this sequence version replaced XM_005267153.4.
            
            Summary: The protein encoded by this gene acts as a protein kinase
            A anchoring protein. Mutations in this gene cause primary ciliary
            dyskinesia; a disorder characterized by defects of the axoneme in
            motile cilia and sperm flagella. The homolog of this gene was first
            identified in the blue-green algae Chlamydomonas as encoding a
            radial spoke protein that formed a structural component of motile
            cilia and flagella. Alternate splicing results in multiple
            transcript variants encoding distinct isoforms. [provided by
            RefSeq, Dec 2016].
            
            Transcript Variant: This variant (2) lacks two exons in the central
            coding region, compared to variant 1, which results in a shorter
            isoform (2), compared to isoform 1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803617.231185.1, AK057655.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-406               AK057931.1         1-406
            407-2029            AK057655.1         1-1623
            2030-5885           AL035530.11        95675-99530
            5886-6283           AI028007.1         1-398               c
FEATURES             Location/Qualifiers
     source          1..6283
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q25.3"
     gene            1..6283
                     /gene="RSPH3"
                     /gene_synonym="CILD32; dJ111C20.1; RSHL2; RSP3"
                     /note="radial spoke head 3"
                     /db_xref="GeneID:83861"
                     /db_xref="HGNC:HGNC:21054"
                     /db_xref="MIM:615876"
     exon            1..753
                     /gene="RSPH3"
                     /gene_synonym="CILD32; dJ111C20.1; RSHL2; RSP3"
                     /inference="alignment:Splign:2.1.0"
     CDS             212..1606
                     /gene="RSPH3"
                     /gene_synonym="CILD32; dJ111C20.1; RSHL2; RSP3"
                     /note="isoform 2 is encoded by transcript variant 2;
                     radial spoke head protein 3 homolog; radial spoke
                     head-like protein 2; radial spoke 3 homolog; A-kinase
                     anchor protein RSPH3; radial spoke head 3 homolog"
                     /codon_start=1
                     /product="radial spoke head protein 3 homolog isoform 2"
                     /protein_id="NP_001333347.1"
                     /db_xref="GeneID:83861"
                     /db_xref="HGNC:HGNC:21054"
                     /db_xref="MIM:615876"
                     /translation="MTVKPAKAASLARNLAKRRRTYLGGAAGRSQEPEVPCAAVLPGK
                     PGDRNCPEFPPPDRTLGCWATDAAPAAGLCGAGSEPSIAPTSCAGNLPSRPPPLLSPL
                     LASRNPCPWHYLHLSGSHNTLAPTCFKAKLHRKRGSQPPDMASALTDRTSRAPSTYTY
                     TSRPRALPCQRSRYRDSLTQPDEEPMHYGNIMYDRRVIRGNTYALQTGPLLFDFDLEV
                     KPVLEVLVGKTIEQSLLEVMEEEELANLRASQREYEELRNSERAEVQRLEEQERRHRE
                     EKERRKKQQWEIMHKHNETSQKIAARAFAQRYLADLLPSVFGSLRDSGYFYDPIERDI
                     EIGFLPWLMNEVEKTMEYSMVGRTVLDMLIREVVEKRLCMYEHGEDTHQSPEPEDEPG
                     GPGAMTESLEASEFLEQSMSQTRELLLDGGYLQRTTYDRRSSQERKFMEERELLGQDE
                     ETAMRKSLGEEELS"
     exon            754..841
                     /gene="RSPH3"
                     /gene_synonym="CILD32; dJ111C20.1; RSHL2; RSP3"
                     /inference="alignment:Splign:2.1.0"
     exon            842..1045
                     /gene="RSPH3"
                     /gene_synonym="CILD32; dJ111C20.1; RSHL2; RSP3"
                     /inference="alignment:Splign:2.1.0"
     exon            1046..1208
                     /gene="RSPH3"
                     /gene_synonym="CILD32; dJ111C20.1; RSHL2; RSP3"
                     /inference="alignment:Splign:2.1.0"
     exon            1209..1295
                     /gene="RSPH3"
                     /gene_synonym="CILD32; dJ111C20.1; RSHL2; RSP3"
                     /inference="alignment:Splign:2.1.0"
     exon            1296..6273
                     /gene="RSPH3"
                     /gene_synonym="CILD32; dJ111C20.1; RSHL2; RSP3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1753..1758
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RSPH3"
                     /gene_synonym="CILD32; dJ111C20.1; RSHL2; RSP3"
                     /note="hexamer: GATAAA"
     polyA_site      1776
                     /gene="RSPH3"
                     /gene_synonym="CILD32; dJ111C20.1; RSHL2; RSP3"
     regulatory      6252..6257
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RSPH3"
                     /gene_synonym="CILD32; dJ111C20.1; RSHL2; RSP3"
                     /note="hexamer: AATAAA"
     polyA_site      6273
                     /gene="RSPH3"
                     /gene_synonym="CILD32; dJ111C20.1; RSHL2; RSP3"
                     /note="major polyA site"
ORIGIN      
        1 acgtaacaga gctgggagcc tggctgccag gggcgtgtta tcgcgggggc agcctgggag
       61 cgctgctgcg ccgcggcagg agaaacagcc gaggacaccc tggaagagca ggagcacgtg
      121 ggcctgcgag aatggcgcca acaccagccc tgcaaagcca cattccaaag ccgggccaag
      181 gacagccacc gttgccccgc aggcctcaag gatgacggtc aagccagcca aggccgcctc
      241 cctagccagg aacctcgcaa agcgcaggcg cacctacctg ggtggcgcgg ctgggcggag
      301 tcaagagccg gaagtccctt gtgccgcagt ccttcccggg aaacctgggg accggaactg
      361 cccggagttc ccgcccccag acagaaccct gggttgctgg gcaaccgatg cggcccccgc
      421 agctggtctc tgtggtgctg ggagcgagcc cagtatagct cccacctcct gcgctggtaa
      481 cctcccgtcc cgcccgcctc ccttgttgtc gcctctcctc gcgtcgcgga atccttgccc
      541 ttggcactac ttacatctct ccgggtccca caacacctta gcgcccacct gcttcaaagc
      601 caagctccac cgaaagcgag gcagtcagcc cccggacatg gcctcagcgc tgactgatcg
      661 cacctctcgg gccccgagca cctacaccta caccagccgg ccccgagcac tgccctgcca
      721 gcgcagccgt taccgggaca gcctgacgca gccagatgaa gaacctatgc attatggaaa
      781 cataatgtat gacagaaggg taattcgagg taacacttat gcactccaga cagggccact
      841 gctctttgac tttgatcttg aagttaaacc agtgttagaa gttttggtgg ggaagacaat
      901 tgagcagtct cttctggaag taatggaaga agaagagctg gctaacctgc gggccagtca
      961 gcgtgagtat gaagaactac ggaatagtga acgtgctgaa gttcaacgac ttgaagagca
     1021 agagaggcga caccgagaag aaaaagaacg gcgtaagaaa cagcagtggg aaataatgca
     1081 caagcacaac gagacatcac aaaaaatcgc cgcccgagca tttgcacagc gttacctggc
     1141 tgaccttctc ccgtctgttt ttggcagcct cagggatagt ggctactttt atgatcccat
     1201 tgaaagagat attgagatag gatttcttcc atggctaatg aatgaagttg aaaaaaccat
     1261 ggaatatagc atggtgggaa gaacagtgct tgacatgttg atccgtgagg tggttgaaaa
     1321 gaggctgtgt atgtatgagc atggggaaga cacacatcag tctccagaac ccgaggatga
     1381 gcctggtggt cctggagcaa tgacagagtc actggaggcc tctgaattcc tggagcagag
     1441 catgtcacag acacgggagc tgcttttaga tggaggctac ctacaaagaa caacatatga
     1501 cagaaggtca tcccaggaaa ggaagtttat ggaagagaga gaactcttag ggcaagatga
     1561 agaaacagca atgaggaagt ccttagggga ggaagaattg tcataggagg gaagcttcaa
     1621 agtcatgaaa ccaatcaaca gccaagtcag caagcaatca ggtaatgatg tgcgagtccc
     1681 ctcaggttat agaaattatg ttgtatttag tcagtatgtc tttttgtgta atggactggg
     1741 ccaatcaaat gtgataaaat taatttgaga atgaaactta aattgaattc atttatttaa
     1801 tagaatattc aaaggctata ttgtaattct aatgagaatt atatttaatt actagctttc
     1861 tattaccatt atatgtaacc agcaaatata tttttcctta gcccttctaa ttttgcgggt
     1921 tagttttttt tagtaagtat gatatttaat gttcaatatc agtgctgtca gccatatagc
     1981 ttttcttctc tataggaaaa ctatttcacc tactgtaaga gtgaatgcct agccaggcgt
     2041 ggtggctcac acctgtaatc ccagcacttt gggaggctga ggctggcaga tcccctgagg
     2101 tcgggagttt gagaccagcc agaccaacaa ggagaaaccc catctctgct aaaaatacaa
     2161 aattagccag gcatggtggc gcatgcctgt aatcctagct acttgggagg ctgaggcagg
     2221 agaatcactt gaacctgggg cggaggttgc ggtgagccaa gtttgcacca ttgcactcca
     2281 gcctgggcaa caagagcaaa actccgtctc aaaaaaaaaa gaaaaaaaag agagagaatg
     2341 cctaaacgtt gtgctactga aagccacagt gctcccttcc tgcaaggacc atcttcaata
     2401 ttcaagtaag gcttaaggaa ttcagtggat tagtatatgc tctcatctat tttttcacat
     2461 tcctactatg aaaaaatttc aggcaatgcc aataaagcaa aagtccctgt ttgccctcat
     2521 acagtctcac tcccctcctc aaaggtagat cactgttatc aggtatgagc tatctaggcc
     2581 ttttttgagg tattcctata caaatgtatt tgaaaatcta gaaatatata gcttgatttt
     2641 ctttatataa atataaacat tatttacata ttttattttt cacttaagag aatgtcttag
     2701 atctttcaag gttagtggca tcaatctctt atcttgtcaa aatatggtta aggtattaca
     2761 tattatgact gtgctgtagc atagggaatt aacatttgac tttttaatct gaatgtgtta
     2821 gagaatttac ctgcagtatt aaaatcacaa gttattttct ctccaaagcc ttcctaatcg
     2881 taagcaataa ctgaagtctt cagagccttt agataaatag agcctatttt tctttttact
     2941 tatttttatt tttatttttt ggagacaagg tctcactctg acacccaggc tggagtgcag
     3001 cagcaccatc acagctcact gcagccttga cctcccaggc tcaggtgatc ttctcacctc
     3061 agccttccaa gtagctggga ctacaggtgt gcaccaccac gtctggctag ttactgtatt
     3121 ttttgtagag atgggctctt gctgtgttgc ccaggctggt ctccaactcc tgggctcaag
     3181 tgatctgccc accttggcct cccaaagtgc taggactaca ggcatgagcc accgcacctg
     3241 gctgagtctg tttttcgcaa aatacttaca catatataaa cgtagtgatt taaaataact
     3301 tataattatt aacccattta ccttaatttc ttttaagtta ggtaccaaga atgtatccac
     3361 atttaaaaaa ttttaaaaga atgcacccat tttcccatat agtatttcac tataacactt
     3421 aggcctcatg gcaaccttct gaagaatgca tgacaatatt tgtatttttt agtttctaat
     3481 ccttaattcg gtcagccagc aaacattagc tgagtgcctg ctctgccttg ggaatgagac
     3541 tgcagataca gcggtgaaca gtcacgaata ctacatgttt tttacagcct agcagtggag
     3601 ctaaatgaag taaatgatgt aaataaagaa accaaagctc aggaatttaa agtgatttgc
     3661 aatagatgac gagcctagta gggaatagga ctaaagctgg aatccaaatc tccttacttc
     3721 taatctggta tactttcttc taataacttg ctgtttttgt gaggtacaaa gaataattta
     3781 aaataaatta ttgcactaat agcattttaa aaattggaat cacgcctctt atccatcatg
     3841 gggctggttc catcttgtta aacagtcttt atgatcatcc taacatttat aagatattaa
     3901 ttttggcaag aagaccaaaa atttttaatt agggctattg ctgattatta ttgttattac
     3961 caattttaaa atatctacaa ctagctttta gaaaccttaa aattgggcca ggcacggtgg
     4021 cttgcacctg taatcccagc actttgggag gccgaggcag gcggatcaca aggtcaggag
     4081 atcaagacca tcctggccaa catggtgaaa ccccgtctct actaaaaata caaaaattag
     4141 tcaggcgtgg tggcgggcac ctgtagtccc agctactcag gaggccgagg caggagaatc
     4201 gcttgaacac gggaggcgga ggttgcagtg agccgagatc gcgccactgc actccagcct
     4261 ggaagacaga gtgagactcc ttctcaaaaa aaaaaaacct taaaattgat ttgaagttga
     4321 ttatctttca taatcttcta attctccttt agaatgatcc agtccattaa acactttgca
     4381 gatgccagac ctatgctcta taaatccata aattaattca atttattgaa agaagtagat
     4441 atctataaac cccacactga actggttaag cttaattgca atcctagtcc atcctagaga
     4501 gaaatcaaaa taattgagag gttagggcat tgtcgctgtg agaaagctta gaggtaacac
     4561 attatatgtt atctggagag aagaatgtta aatggaaaca aagtttatga agaatttctt
     4621 caagtacctg aaggactttc tgggaaatta caagatagca ttcttaggtt ccatatgtct
     4681 agaagacttt tcataactgt aagcaacttt tgcaaatggt ttgggaacat aaaactcttc
     4741 cagcatagaa gaaaatgggt actattaaca ttcagatggt tatgggacca tcataacagt
     4801 ttgatgtgat atgcaaaatg ctacatcccc aactatgacc ctcagtatga cttttaaagg
     4861 aattataagc tccaaatact ttttggagta taaacagtac ttgacttttt cacacatttg
     4921 ctaataccct tctcataagt tttatttctt tctgatgttt cagctaacac tgagttaaca
     4981 gaagctaaat aataataaaa tgaagaacag atagtaaata tattgaaatt gtcattcctg
     5041 gccgggtgtg gtggcttacg cctataatcc cagcactttg ggaggccaag gcaggcagat
     5101 cacgaggtca ggagatcgag gccatcctgg ccaacatggt gaaaccctgt ctccactaaa
     5161 aatacaaaaa ttagctgggt atggtgacgc atgcctataa tcccagctac tcgggagact
     5221 gaggcataag aatcacttga acccaggagg tcgcgccgct gcactccagc ccggtgacag
     5281 agcaagactc cgtctcaaaa aaaaaaaaaa gaaagaaaaa gaaattgtca ttcttaacat
     5341 aaattcccat atcactaaaa tagatcagag aggtagaatg aaattttctc tagcataggc
     5401 atcattacat ttaagccatc tgagggctat gagcgtggcc tgcaaagtca ctctgagcaa
     5461 cacaaggtgg agtttgaaag cagattcaaa gccagaccac tgggcttgag tcctcacatg
     5521 caagcatgta gtcttcacca tggagaaggg ggtttgcctt tctcaaagct tttttctttt
     5581 aaattaagaa aaagaactga tttatcagtc tagtgtttca ttttaaaaac atatgaaagg
     5641 gtgggtaact tgcagaagaa agtatttgga gcctataatt ccattaaaag ttataatggc
     5701 ggtcatagtt ggggaagcag cattttgcat attatatcaa aatgttatgg taccataacc
     5761 atccaaatgt taatggtacc aatttcttct atcctagaaa catttcattt tatgttgttg
     5821 aaacataaca actatatcag ctagattttt tttctatgct ttacctgcta tggaaaattt
     5881 gacacattct gctttactct tttgtttata ggtgaatcac agaatgtatt tttatgtatt
     5941 ctgtagttca atagccatgg ctgtttactt catttaattt atttagcata aagacattat
     6001 gaaaaggcct aaacatgagc ttcacttccc cactaactaa ttagcatctg ttatttctta
     6061 accgtaatgc ctaggcctaa cctcaaccaa ccttccacaa tcttgttcac agtcagagac
     6121 ccaaaaaaga gtgttataag tggggtgtca ctattgtgaa agtagtgcta atggtgttga
     6181 attggtacct atgattcttc ttttgaatta gattgtatgt ttcatgatct caacttacat
     6241 tactaaaatt caataaactg atcatttaga tgaaaaaaaa aaa
//
