LOCUS       NM_001099697            3112 bp    mRNA    linear   PRI 20-FEB-2025
DEFINITION  Homo sapiens radial spoke head 10 homolog B2 (RSPH10B2), mRNA.
ACCESSION   NM_001099697 XM_001127254
VERSION     NM_001099697.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Pineau,C., Hikmet,F., Zhang,C., Oksvold,P., Chen,S., Fagerberg,L.,
            Uhlen,M. and Lindskog,C.
  TITLE     Cell Type-Specific Expression of Testis Elevated Genes Based on
            Transcriptomics and Antibody-Based Proteomics
  JOURNAL   J Proteome Res 18 (12), 4215-4230 (2019)
   PUBMED   31429579
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Zhu,X., Liu,Y. and Yang,P.
  TITLE     Radial Spokes-A Snapshot of the Motility Regulation, Assembly, and
            Evolution of Cilia and Flagella
  JOURNAL   Cold Spring Harb Perspect Biol 9 (5), a028126 (2017)
   PUBMED   27940518
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3112)
  AUTHORS   Pigino,G. and Ishikawa,T.
  TITLE     Axonemal radial spokes: 3D structure, function and assembly
  JOURNAL   Bioarchitecture 2 (2), 50-58 (2012)
   PUBMED   22754630
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC073343.6 and AC079882.6.
            
            On Jun 24, 2021 this sequence version replaced NM_001099697.1.
            
            Summary: This gene encodes a protein component of the radial spoke
            head in flagella and motile cilia. Eukaryotic flagella and motile
            cilia share a common 9 + 2 structure, in which nine peripheral
            microtubule doublets (MTDs) surround a central-pair of microtubules
            (CP), with radial spokes connecting the MTDs to the CP. The radial
            spoke is a multi-protein complex that works as a mechanochemical
            transducer between the CP and the MTDs. The radial spoke
            contributes to the regulation of the activity of dynein motors, and
            thus to flagellar motility. PMID: 22754630 provides a good review
            of radial spokes. [provided by RefSeq, Jul 2017].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR18074969.3314788.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMEA2148093
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000404077.6/ ENSP00000386102.1
            RefSeq Select criteria :: based on manual assertion, conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-72                AC073343.6         139343-139414
            73-192              AC073343.6         142597-142716
            193-531             AC073343.6         142833-143171
            532-607             AC073343.6         144324-144399
            608-676             AC073343.6         145466-145534
            677-850             AC073343.6         149168-149341
            851-936             AC073343.6         150946-151031
            937-1057            AC073343.6         151997-152117
            1058-1234           AC073343.6         153830-154006
            1235-1379           AC073343.6         156787-156931
            1380-1501           AC073343.6         158590-158711
            1502-1691           AC073343.6         161597-161786
            1692-1806           AC073343.6         164850-164964
            1807-1886           AC073343.6         166049-166128
            1887-2035           AC073343.6         166568-166716
            2036-2143           AC073343.6         171189-171296
            2144-2287           AC079882.6         151-294
            2288-2416           AC079882.6         2904-3032
            2417-2510           AC079882.6         5177-5270
            2511-2709           AC079882.6         9841-10039
            2710-3112           AC079882.6         11636-12038
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7p22.1"
     gene            1..3112
                     /gene="RSPH10B2"
                     /note="radial spoke head 10 homolog B2"
                     /db_xref="GeneID:728194"
                     /db_xref="HGNC:HGNC:34385"
     exon            1..72
                     /gene="RSPH10B2"
                     /inference="alignment:Splign:2.1.0"
     exon            73..192
                     /gene="RSPH10B2"
                     /inference="alignment:Splign:2.1.0"
     exon            193..531
                     /gene="RSPH10B2"
                     /inference="alignment:Splign:2.1.0"
     CDS             278..2890
                     /gene="RSPH10B2"
                     /codon_start=1
                     /product="radial spoke head 10 homolog B2"
                     /protein_id="NP_001093167.1"
                     /db_xref="CCDS:CCDS43552.1"
                     /db_xref="GeneID:728194"
                     /db_xref="HGNC:HGNC:34385"
                     /translation="MVKEKKKADKKGEKSARSPSSLSDNLDFSKQDGNTTRQEMSPAG
                     VPLLGMQLNEVKPKKDRQNVQQNEDASQYEESILTKLIVESYEGEKVRGLYEGEGFAA
                     FQGGCTYRGMFSEGLMHGQGTYIWADGLKYEGDFVKNVPMNHGVYTWPDGSMYEGEVV
                     NGMRNGFGMFKCSTQPVSYIGHWCNGKRHGKGSIYYNQEGTCWYEGDWVQNIKKGWGI
                     RCYKSGNIYEGQWEDNMRHGEGRMRWLTTNEEYTGRWERGIQNGFGTHTWFLKRIRSS
                     QYPLRNEYIGEFVNGYRHGRGKFYYASGAMYDGEWVSNKKHGMGRLTFKNGRVYEGAF
                     SNDHIAGFPDLEVEFISCLDLSSGVAPRLSRSAELIRKLDGSESHSVLGSSIELDLNL
                     LLDMYPETVQPEEKKQVEYAVLRNITELRRIYSFYSSLGCGHSLDNTFLMTKLHFWRF
                     LKDCKFHHHKLTLADMDRILSANNDIPVEEIHSPFTTILLRTFLNYLLHLAYHIYHEE
                     FQKRSPSLFLCFTKLMTENIRPNACQIKGNLFREQQRTLYSMSYMNKCWEIYLAYCRP
                     SAAPPHEPTMKMRHFLWMLKDFKMINKELTAATFMEVIAEDNRFIYDGIDSNFEPELV
                     FLEFFEALLSFAFICVTDQMTKSYTNVPADDVSGNKHETIYTILNQDAQNKSPSAVMS
                     HESDAAHSDSARSSSSKLELSPDVNKIRKSEPKIKKSVSHERVSKMNFKLTGKGITFF
                     SSESKKYERPKDDREEEFNTWVNNTYVFFVNTLFHAYKREEAIKEKIRADRLRSTAQA
                     QQRKMEDDELEARLNIFILREEEAKRHDYEVDITVLKEPADVSSSHLILDPPKEDVTV
                     SPSSKTITSKKKKK"
     exon            532..607
                     /gene="RSPH10B2"
                     /inference="alignment:Splign:2.1.0"
     exon            608..676
                     /gene="RSPH10B2"
                     /inference="alignment:Splign:2.1.0"
     exon            677..850
                     /gene="RSPH10B2"
                     /inference="alignment:Splign:2.1.0"
     exon            851..936
                     /gene="RSPH10B2"
                     /inference="alignment:Splign:2.1.0"
     exon            937..1057
                     /gene="RSPH10B2"
                     /inference="alignment:Splign:2.1.0"
     exon            1058..1234
                     /gene="RSPH10B2"
                     /inference="alignment:Splign:2.1.0"
     exon            1235..1379
                     /gene="RSPH10B2"
                     /inference="alignment:Splign:2.1.0"
     exon            1380..1501
                     /gene="RSPH10B2"
                     /inference="alignment:Splign:2.1.0"
     exon            1502..1691
                     /gene="RSPH10B2"
                     /inference="alignment:Splign:2.1.0"
     exon            1692..1806
                     /gene="RSPH10B2"
                     /inference="alignment:Splign:2.1.0"
     exon            1807..1886
                     /gene="RSPH10B2"
                     /inference="alignment:Splign:2.1.0"
     exon            1887..2035
                     /gene="RSPH10B2"
                     /inference="alignment:Splign:2.1.0"
     exon            2036..2143
                     /gene="RSPH10B2"
                     /inference="alignment:Splign:2.1.0"
     exon            2144..2287
                     /gene="RSPH10B2"
                     /inference="alignment:Splign:2.1.0"
     exon            2288..2416
                     /gene="RSPH10B2"
                     /inference="alignment:Splign:2.1.0"
     exon            2417..2510
                     /gene="RSPH10B2"
                     /inference="alignment:Splign:2.1.0"
     exon            2511..2709
                     /gene="RSPH10B2"
                     /inference="alignment:Splign:2.1.0"
     exon            2710..3112
                     /gene="RSPH10B2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3091..3096
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RSPH10B2"
                     /note="hexamer: ATTAAA"
     polyA_site      3112
                     /gene="RSPH10B2"
                     /note="major polyA site"
ORIGIN      
        1 gggtctggga cgccgagagc ccggcaacac ctcagcccga gcccggcgag gtctctgggc
       61 tcctggagcg agagaggtgg catctcacta cgtggcctag gctgatctcc ctctcctgga
      121 ctcacatgat cctcccacct tggcccttca acgtgctgag atcgcaggtg tgagccacca
      181 cacccggccc tgataaaact catcagtgct ttgaaacaag cagctctgaa ccaaagaaat
      241 catttgattc aagtaaccat tggatcttcc caaggcaatg gtgaaagaaa agaaaaaagc
      301 agacaaaaaa ggggagaagt ctgcccgctc tccctcatct ctctctgata atctagactt
      361 ttccaaacaa gatggcaaca ccactaggca agagatgtcc ccagctggtg tcccattgct
      421 gggaatgcag ctcaacgaag tgaaacccaa aaaagaccgc caaaacgttc agcagaacga
      481 agatgccagc caatacgaag agtccattct gaccaaactc atagtggaaa gctatgaagg
      541 ggaaaaggtt cgtgggctgt atgagggaga aggcttcgca gcctttcaag gcggttgtac
      601 ctatcgtggt atgttttcag aaggactcat gcatggacag gggacttata tttgggccga
      661 tggattaaaa tatgagggcg actttgtgaa gaatgtcccg atgaaccacg gcgtgtacac
      721 gtggccggac ggcagcatgt atgaaggcga agtggtcaac ggcatgagga acggattcgg
      781 gatgttcaag tgcagcaccc agcctgtgtc ctacatcggc cactggtgca atggcaagcg
      841 gcacgggaag ggctccattt attacaatca agagggtacg tgttggtacg agggagactg
      901 ggtacaaaac atcaaaaagg gctggggaat aagatgttat aaatctggaa atatatacga
      961 aggccagtgg gaagacaaca tgcgccacgg ggaggggagg atgaggtggc tgaccaccaa
     1021 cgaagagtac accgggcggt gggagagggg catccagaat ggctttggaa cacacacatg
     1081 gtttctaaag agaatccgca gttcccagta tcctttgaga aatgaataca taggggagtt
     1141 tgtaaatgga tatcgtcacg gacgtggcaa gttttattat gccagtggag ccatgtatga
     1201 tggagaatgg gtttccaata agaaacatgg catgggccga ttaactttca agaacgggcg
     1261 tgtgtacgaa ggcgcattct ccaatgacca catagctggg tttccggatc ttgaagttga
     1321 attcatcagc tgcctggacc tgtcttcagg agttgcccca agactgtcca ggagcgccga
     1381 actgatcaga aagcttgatg gcagtgaaag tcattctgtg ttgggatcga gcattgagct
     1441 ggatctaaat ttgctcctgg acatgtaccc tgagacagtc caacctgaag aaaagaagca
     1501 ggtggaatat gccgtcttaa gaaatattac agaattaaga agaatttaca gcttttacag
     1561 cagcctggga tgcggccact ctctggataa tacctttctg atgacaaagc ttcacttctg
     1621 gagatttcta aaagattgca aatttcatca ccacaaacta actcttgctg atatggacag
     1681 gatattaagt gccaataatg acataccagt tgaagaaatc cattctccat ttacaacaat
     1741 acttttgaga acatttttga attacctcct gcatttggcg taccacattt atcatgaaga
     1801 attccaaaag agaagcccat ccctcttctt gtgttttaca aaactgatga ccgagaacat
     1861 tcgtccaaat gcctgccaga taaaaggcaa tttattccgt gagcaacagc ggacgctcta
     1921 ctctatgagt tacatgaata agtgctggga gatttatctc gcttactgca gacccagtgc
     1981 agcgcctccc cacgagccta cgatgaagat gagacacttc ctctggatgc tgaaagactt
     2041 taaaatgata aataaagaat taacagcagc tacatttatg gaggtcatag cagaggataa
     2101 tcgtttcata tatgatggaa ttgacagcaa ctttgaacct gagctggttt tcctggaatt
     2161 ctttgaagct ctcttaagct ttgcattcat ctgtgttact gaccaaatga ctaaatccta
     2221 tacaaatgtt ccagctgatg atgtgtctgg aaataaacat gaaactattt atacaatact
     2281 aaatcaggac gcccagaaca agagtcccag cgcggtcatg agccacgaat cggatgctgc
     2341 tcactctgac agtgccaggt catcttccag caagttagaa ctctcgcctg atgttaacaa
     2401 aataaggaaa tcagagccca agatcaagaa gtctgtaagt catgaaagag tctccaaaat
     2461 gaattttaaa ttgactggaa aagggatcac ctttttctca tctgagagca agaaatatga
     2521 gagacccaag gatgatcgag aggaagagtt caacacgtgg gtcaataata cgtacgtctt
     2581 ctttgtgaac acgctctttc atgcgtataa acgtgaagaa gctatcaagg agaaaataag
     2641 ggcagacagg ttacgtagca cagcacaggc ccagcagcgg aagatggaag atgacgaact
     2701 ggaagcaagg ctgaacatct ttatcttgag agaggaagag gccaagagac atgactatga
     2761 ggtggacatc acagtgctca aggagccggc agacgtgtca tcctctcacc tcatactgga
     2821 ccctcccaag gaggatgtga ccgtgtcccc atccagcaag accatcacca gcaagaagaa
     2881 gaaaaagtag agagacacga ggacacacac acaggcttag gatggcacgt gctgtaagat
     2941 ttcctgagca ttacagttct tagcactgaa ctcgtcacag tgttttaaca taccccttgg
     3001 cccactgcat ctcaggtttc ctacatggct ggattacggg agttcgagac cagcctgggc
     3061 aacatagtga gaccccccca tctctgcaaa attaaaacat cagccgaccg tg
//
