LOCUS       NM_080657               3407 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens radical S-adenosyl methionine domain containing 2
            (RSAD2), transcript variant 1, mRNA.
ACCESSION   NM_080657
VERSION     NM_080657.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   Xin,Y., He,Z., Mei,Y., Li,X., Zhao,Z., Zhao,M., Yang,M. and Wu,H.
  TITLE     Interferon-alpha regulates abnormally increased expression of RSAD2
            in Th17 and Tfh cells in systemic lupus erythematosus patients
  JOURNAL   Eur J Immunol 53 (8), e2350420 (2023)
   PUBMED   37179450
  REMARK    GeneRIF: Interferon-alpha regulates abnormally increased expression
            of RSAD2 in Th17 and Tfh cells in systemic lupus erythematosus
            patients.
REFERENCE   2  (bases 1 to 3407)
  AUTHORS   Wilson,M.R., Harkins,S., Reske,J.J., Siwicki,R.A., Adams,M.,
            Bae-Jump,V.L., Teixeira,J.M. and Chandler,R.L.
  TITLE     PIK3CA mutation in endometriotic epithelial cells promotes
            viperin-dependent inflammatory response to insulin
  JOURNAL   Reprod Biol Endocrinol 21 (1), 43 (2023)
   PUBMED   37170094
  REMARK    GeneRIF: PIK3CA mutation in endometriotic epithelial cells promotes
            viperin-dependent inflammatory response to insulin.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3407)
  AUTHORS   Weinstein,A.G., Godet,I. and Gilkes,D.M.
  TITLE     The rise of viperin: the emerging role of viperin in cancer
            progression
  JOURNAL   J Clin Invest 132 (24), e165907 (2022)
   PUBMED   36519538
  REMARK    GeneRIF: The rise of viperin: the emerging role of viperin in
            cancer progression.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3407)
  AUTHORS   Sarkar,R., Nandi,S., Lo,M., Gope,A. and Chawla-Sarkar,M.
  TITLE     Viperin, an IFN-Stimulated Protein, Delays Rotavirus Release by
            Inhibiting Non-Structural Protein 4 (NSP4)-Induced Intrinsic
            Apoptosis
  JOURNAL   Viruses 13 (7), 1324 (2021)
   PUBMED   34372530
  REMARK    GeneRIF: Viperin, an IFN-Stimulated Protein, Delays Rotavirus
            Release by Inhibiting Non-Structural Protein 4 (NSP4)-Induced
            Intrinsic Apoptosis.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3407)
  AUTHORS   Khantisitthiporn,O., Shue,B., Eyre,N.S., Nash,C.W., Turnbull,L.,
            Whitchurch,C.B., Van der Hoek,K.H., Helbig,K.J. and Beard,M.R.
  TITLE     Viperin interacts with PEX19 to mediate peroxisomal augmentation of
            the innate antiviral response
  JOURNAL   Life Sci Alliance 4 (7), e202000915 (2021)
   PUBMED   34108265
  REMARK    GeneRIF: Viperin interacts with PEX19 to mediate peroxisomal
            augmentation of the innate antiviral response.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3407)
  AUTHORS   Severa,M., Coccia,E.M. and Fitzgerald,K.A.
  TITLE     Toll-like receptor-dependent and -independent viperin gene
            expression and counter-regulation by PRDI-binding factor-1/BLIMP1
  JOURNAL   J Biol Chem 281 (36), 26188-26195 (2006)
   PUBMED   16849320
  REMARK    GeneRIF: Results identify Viperin as a tightly regulated ISGF3
            target gene, which is counter-regulated by PRDI-BF1.
REFERENCE   7  (bases 1 to 3407)
  AUTHORS   Helbig,K.J., Lau,D.T., Semendric,L., Harley,H.A. and Beard,M.R.
  TITLE     Analysis of ISG expression in chronic hepatitis C identifies
            viperin as a potential antiviral effector
  JOURNAL   Hepatology 42 (3), 702-710 (2005)
   PUBMED   16108059
  REMARK    GeneRIF: ISG (interferon-stmiulated genese) viperin has
            anti-Hepatitis c virus activity in vitro; we postulate that
            viperin, and other ISGs, acts to limit HCV replication.
REFERENCE   8  (bases 1 to 3407)
  AUTHORS   Olofsson,P.S., Jatta,K., Wagsater,D., Gredmark,S., Hedin,U.,
            Paulsson-Berne,G., Soderberg-Naucler,C., Hansson,G.K. and Sirsjo,A.
  TITLE     The antiviral cytomegalovirus inducible gene 5/viperin is expressed
            in atherosclerosis and regulated by proinflammatory agents
  JOURNAL   Arterioscler Thromb Vasc Biol 25 (7), e113-e116 (2005)
   PUBMED   15890971
  REMARK    GeneRIF: Viperin is expressed in atherosclerosis and induced in
            vascular cells by inflammatory stimuli and cytomegalovirus
            infection
REFERENCE   9  (bases 1 to 3407)
  AUTHORS   Chin,K.C. and Cresswell,P.
  TITLE     Viperin (cig5), an IFN-inducible antiviral protein directly induced
            by human cytomegalovirus
  JOURNAL   Proc Natl Acad Sci U S A 98 (26), 15125-15130 (2001)
   PUBMED   11752458
  REMARK    Erratum:[Proc Natl Acad Sci U S A 2002 Feb 19;99(4):2460]
REFERENCE   10 (bases 1 to 3407)
  AUTHORS   Zhu,H., Cong,J.P. and Shenk,T.
  TITLE     Use of differential display analysis to assess the effect of human
            cytomegalovirus infection on the accumulation of cellular RNAs:
            induction of interferon-responsive RNAs
  JOURNAL   Proc Natl Acad Sci U S A 94 (25), 13985-13990 (1997)
   PUBMED   9391139
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC017969.1, AF442151.1 and
            AC017076.14.
            
            On Nov 23, 2018 this sequence version replaced NM_080657.4.
            
            Summary: The protein encoded by this gene is an
            interferon-inducible antiviral protein that belongs to the
            S-adenosyl-L-methionine (SAM) superfamily of enzymes. The protein
            plays a role in cellular antiviral response and innate immune
            signaling. Antiviral effects result from inhibition of viral RNA
            replication, interference in the secretory pathway, binding to
            viral proteins and dysregulation of cellular lipid metabolism. The
            protein has been found to inhibit both DNA and RNA viruses,
            including influenza virus, human immunodeficiency virus (HIV-1) and
            Zika virus. [provided by RefSeq, Sep 2020].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF442151.1, SRR1660803.248205.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            coronavirus related    :: involved in immune response or antiviral
                                      activity
            MANE Ensembl match     :: ENST00000382040.4/ ENSP00000371471.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-148               BC017969.1         1-148
            149-1508            AF442151.1         149-1508
            1509-3400           AC017076.14        95774-97665
            3401-3407           AC017076.14        97666-97672
FEATURES             Location/Qualifiers
     source          1..3407
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2p25.2"
     gene            1..3407
                     /gene="RSAD2"
                     /gene_synonym="cig33; cig5; SAND; vig1"
                     /note="radical S-adenosyl methionine domain containing 2"
                     /db_xref="GeneID:91543"
                     /db_xref="HGNC:HGNC:30908"
                     /db_xref="MIM:607810"
     exon            1..370
                     /gene="RSAD2"
                     /gene_synonym="cig33; cig5; SAND; vig1"
                     /inference="alignment:Splign:2.1.0"
     CDS             25..1110
                     /gene="RSAD2"
                     /gene_synonym="cig33; cig5; SAND; vig1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     viperin; radical S-adenosyl methionine domain-containing
                     protein 2; cytomegalovirus-induced gene 5 protein; virus
                     inhibitory protein, endoplasmic reticulum-associated,
                     interferon-inducible; S-adenosylmethionine-dependent
                     nucleotide dehydratase RSAD2"
                     /codon_start=1
                     /product="S-adenosylmethionine-dependent nucleotide
                     dehydratase RSAD2 isoform 1"
                     /protein_id="NP_542388.2"
                     /db_xref="CCDS:CCDS1656.1"
                     /db_xref="GeneID:91543"
                     /db_xref="HGNC:HGNC:30908"
                     /db_xref="MIM:607810"
                     /translation="MWVLTPAAFAGKLLSVFRQPLSSLWRSLVPLFCWLRATFWLLAT
                     KRRKQQLVLRGPDETKEEEEDPPLPTTPTSVNYHFTRQCNYKCGFCFHTAKTSFVLPL
                     EEAKRGLLLLKEAGMEKINFSGGEPFLQDRGEYLGKLVRFCKVELRLPSVSIVSNGSL
                     IRERWFQNYGEYLDILAISCDSFDEEVNVLIGRGQGKKNHVENLQKLRRWCRDYRVAF
                     KINSVINRFNVEEDMTEQIKALNPVRWKVFQCLLIEGENCGEDALREAERFVIGDEEF
                     ERFLERHKEVSCLVPESNQKMKDSYLILDEYMRFLNCRKGRKDPSKSILDVGVEEAIK
                     FSGFDEKMFLKRGGKYIWSKADLKLDW"
     exon            371..532
                     /gene="RSAD2"
                     /gene_synonym="cig33; cig5; SAND; vig1"
                     /inference="alignment:Splign:2.1.0"
     exon            533..762
                     /gene="RSAD2"
                     /gene_synonym="cig33; cig5; SAND; vig1"
                     /inference="alignment:Splign:2.1.0"
     exon            763..912
                     /gene="RSAD2"
                     /gene_synonym="cig33; cig5; SAND; vig1"
                     /inference="alignment:Splign:2.1.0"
     exon            913..945
                     /gene="RSAD2"
                     /gene_synonym="cig33; cig5; SAND; vig1"
                     /inference="alignment:Splign:2.1.0"
     exon            946..3407
                     /gene="RSAD2"
                     /gene_synonym="cig33; cig5; SAND; vig1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3380..3385
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RSAD2"
                     /gene_synonym="cig33; cig5; SAND; vig1"
                     /note="hexamer: AATAAA"
     polyA_site      3407
                     /gene="RSAD2"
                     /gene_synonym="cig33; cig5; SAND; vig1"
                     /note="major polyA site"
ORIGIN      
        1 gctctgctcc aggcatctgc cacaatgtgg gtgcttacac ctgctgcttt tgctgggaag
       61 ctcttgagtg tgttcaggca acctctgagc tctctgtgga ggagcctggt cccgctgttc
      121 tgctggctga gggcaacctt ctggctgcta gctaccaaga ggagaaagca gcagctggtc
      181 ctgagagggc cagatgagac caaagaggag gaagaggacc ctcctctgcc caccacccca
      241 accagcgtca actatcactt cactcgccag tgcaactaca aatgcggctt ctgtttccac
      301 acagccaaaa catcctttgt gctgcccctt gaggaagcaa agagaggatt gcttttgctt
      361 aaggaagctg gtatggagaa gatcaacttt tcaggtggag agccatttct tcaagaccgg
      421 ggagaatacc tgggcaagtt ggtgaggttc tgcaaagtag agttgcggct gcccagcgtg
      481 agcatcgtga gcaatggaag cctgatccgg gagaggtggt tccagaatta tggtgagtat
      541 ttggacattc tcgctatctc ctgtgacagc tttgacgagg aagtcaatgt ccttattggc
      601 cgtggccaag gaaagaagaa ccatgtggaa aaccttcaaa agctgaggag gtggtgtagg
      661 gattatagag tcgctttcaa gataaattct gtcattaatc gtttcaacgt ggaagaggac
      721 atgacggaac agatcaaagc actaaaccct gtccgctgga aagtgttcca gtgcctctta
      781 attgagggtg agaattgtgg agaagatgct ctaagagaag cagaaagatt tgttattggt
      841 gatgaagaat ttgaaagatt cttggagcgc cacaaagaag tgtcctgctt ggtgcctgaa
      901 tctaaccaga agatgaaaga ctcctacctt attctggatg aatatatgcg ctttctgaac
      961 tgtagaaagg gacggaagga cccttccaag tccatcctgg atgttggtgt agaagaagct
     1021 ataaaattca gtggatttga tgaaaagatg tttctgaagc gaggaggaaa atacatatgg
     1081 agtaaggctg atctgaagct ggattggtag agcggaaagt ggaacgagac ttcaacacac
     1141 cagtgggaaa actcctagag taactgccat tgtctgcaat actatcccgt tggtatttcc
     1201 cagtggctga aaacctgatt ttctgctgca cgtggcatct gattacctgt ggtcactgaa
     1261 cacacgaata acttggatag caaatcctga gacaatggaa aaccattaac tttacttcat
     1321 tggcttataa ccttgttgtt attgaaacag cacttctgtt tttgagtttg ttttagctaa
     1381 aaagaaggaa tacacacagg aataatgacc ccaaaaatgc ttagataagg cccctataca
     1441 caggacctga catttagctc aatgatgcgt ttgtaagaaa taagctctag tgatatctgt
     1501 gggggcaaaa tttaatttgg atttgatttt ttaaaacaat gtttactgcg atttctatat
     1561 ttccattttg aaactatttc ttgttccagg tttgttcatt tgacagagtc agtatttttt
     1621 gccaaatatc cagataacca gttttcacat ctgagacatt acaaagtatc tgcctcaatt
     1681 atttctgctg gttataatgc tttttttttt ttgcctttat gccattgcag tcttgtactt
     1741 tttactgtga tgtacagaaa tagtcaacag atgtttccaa gaacatatga tatgataatc
     1801 ctaccaattt tcaagaagtc tctagaaaga gataacacat ggaaagacgg tgtggtgcag
     1861 cccagcccac ggtggctgtt ccatgaatgc tggctaccta tgtgtgtggt acctgttgtg
     1921 tccctttctc ttcaaagatc ctgagcaaaa caaagatacg ctttccattt gatgatggag
     1981 ttgacatgga ggcagtgctt gcattgcttt gttcgcctat catctggcca catgaggctg
     2041 tcaagcaaaa gaataggagt gtagttgagt agctggttgg ccctacatct ctgagaagtg
     2101 acggcacact gggttggcat aagatatcct aaaatcacgc tggaaccttg ggcaaggaag
     2161 aatgtgagca agagtagaga gagtgcctgg atttcatgtc agtgaagcca agtcaccata
     2221 tcatattttt gaatgaactc tgagtcagtt gaaatagggt accatctagg tcagtttaag
     2281 aagagtcagc tcagagaaag caagcataag ggaaaatgtc acgtaaacta gatcagggaa
     2341 caaaatcctc tccttgtgga aatatcccat gcagtttgtt gatacaactt agtatcttat
     2401 tgcctaaaaa aaaatttctt atcattgttt caaaaaagca aaatcatgga aaatttttgt
     2461 tgtccaggca aataaaaggt cattttaatt tagctgcaat ttcagtgttc ctcactaggt
     2521 ggcatttaaa tgtcgcctga tgtcattaag caccatccaa aaagtctgct tcataatcta
     2581 ttttcaagac ttggtgattc tgaaagtttt ggtttttgtg actttgtttc tcaggaaaaa
     2641 aaatattcct acttaaattt taagtctata attcaattta aatatgtgtg tgtctcatcc
     2701 aggataggat aggttgtctt ctattttcca ttttacctat ttactttttt tgtaagaaaa
     2761 gagaaaaatg aattctaaag atgttcccca tgggttttga ttgtgtctaa gctatgatga
     2821 ccttcatata atcagcataa acataaaaca aattttttac ttaacatgag tgcactttac
     2881 taatcctcat ggcacagtgg ctcacgcctg taatcccagc acttgggagg acaatgtggg
     2941 tggatcacga ggtcaggagt tcgagaacag cctggccaac atggtgaaac cccgtctcca
     3001 ctaaaaatac aaaaattagc caggcatggt ggcgtacact tgtaattcca gctactcaag
     3061 aggctgaggc aggaggattg cttgaaccct gaaggcagag gttacagagc caagatagcg
     3121 ccactgcact ccagcctgga tgacagagca agactccgtc tcaaaaaaaa aaaaaaaaaa
     3181 aagcaagaga gttcaactaa gaaaggtcac atatgtgaaa gcccaaggac actgtttgat
     3241 atacagcagg tattcaatca gtgttatttg aaaccaaatc tgaatttgaa gtttgaatct
     3301 tctgagttgg aatgaatttt tttctagctg agggaaactg tatttttctt tccccaaaga
     3361 ggaatgtaat gtaaagtgaa ataaaactat aagctatgtt aaataca
//
