LOCUS       NM_012250               2343 bp    mRNA    linear   PRI 08-OCT-2024
DEFINITION  Homo sapiens RAS related 2 (RRAS2), transcript variant 1, mRNA.
ACCESSION   NM_012250
VERSION     NM_012250.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Cifuentes,C., Oeste,C.L., Fernandez-Pisonero,I., Hortal,A.M.,
            Garcia-Macias,C., Hochart,J., Rubira,R., Horndler,L., Horndler,C.,
            Bustelo,X.R. and Alarcon,B.
  TITLE     Unmutated RRAS2 emerges as a key oncogene in post-partum-associated
            triple negative breast cancer
  JOURNAL   Mol Cancer 23 (1), 142 (2024)
   PUBMED   38987766
  REMARK    GeneRIF: Unmutated RRAS2 emerges as a key oncogene in
            post-partum-associated triple negative breast cancer.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Zeng,L., Wang,J., Zhu,H., Huang,Y., Deng,Y., Wei,P., Nie,J.,
            Tang,B., Chen,A. and Zhu,S.
  TITLE     The RRAS2 pathogenic variant (c.67G>T; p. Gly23Cys) produces Noonan
            syndrome with embryonal rhabdomyosarcoma
  JOURNAL   Mol Genet Genomic Med 12 (1), e2313 (2024)
   PUBMED   37942564
  REMARK    GeneRIF: The RRAS2 pathogenic variant (c.67G>T; p. Gly23Cys)
            produces Noonan syndrome with embryonal rhabdomyosarcoma.
REFERENCE   3  (bases 1 to 2343)
  AUTHORS   Sun,C., Mahapatra,K.D., Elton,J., Li,C., Fernando,W.,
            Lohcharoenkal,W., Lapins,J., Homey,B., Sonkoly,E. and Pivarcsi,A.
  TITLE     MicroRNA-23b Plays a Tumor-Suppressive Role in Cutaneous Squamous
            Cell Carcinoma and Targets Ras-Related Protein RRAS2
  JOURNAL   J Invest Dermatol 143 (12), 2386-2396 (2023)
   PUBMED   37423552
  REMARK    GeneRIF: MicroRNA-23b Plays a Tumor-Suppressive Role in Cutaneous
            Squamous Cell Carcinoma and Targets Ras-Related Protein RRAS2.
REFERENCE   4  (bases 1 to 2343)
  AUTHORS   Lacuna,M., Hortal,A.M., Cifuentes,C., Gonzalo,T., Alcoceba,M.,
            Bastos,M., Bustelo,X.R., Gonzalez,M. and Alarcon,B.
  TITLE     Characterization of Three Somatic Mutations in the 3'UTR of RRAS2
            and Their Inverse Correlation with Lymphocytosis in Chronic
            Lymphocytic Leukemia
  JOURNAL   Cells 12 (23), 2687 (2023)
   PUBMED   38067115
  REMARK    GeneRIF: Characterization of Three Somatic Mutations in the 3'UTR
            of RRAS2 and Their Inverse Correlation with Lymphocytosis in
            Chronic Lymphocytic Leukemia.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2343)
  AUTHORS   Ehrhardt,G.R., Leslie,K.B., Lee,F., Wieler,J.S. and Schrader,J.W.
  TITLE     M-Ras, a widely expressed 29-kD homologue of p21 Ras: expression of
            a constitutively active mutant results in factor-independent growth
            of an interleukin-3-dependent cell line
  JOURNAL   Blood 94 (7), 2433-2444 (1999)
   PUBMED   10498616
REFERENCE   6  (bases 1 to 2343)
  AUTHORS   Linnemann,T., Geyer,M., Jaitner,B.K., Block,C., Kalbitzer,H.R.,
            Wittinghofer,A. and Herrmann,C.
  TITLE     Thermodynamic and kinetic characterization of the interaction
            between the Ras binding domain of AF6 and members of the Ras
            subfamily
  JOURNAL   J Biol Chem 274 (19), 13556-13562 (1999)
   PUBMED   10224125
REFERENCE   7  (bases 1 to 2343)
  AUTHORS   Rosario,M., Paterson,H.F. and Marshall,C.J.
  TITLE     Activation of the Raf/MAP kinase cascade by the Ras-related protein
            TC21 is required for the TC21-mediated transformation of NIH 3T3
            cells
  JOURNAL   EMBO J 18 (5), 1270-1279 (1999)
   PUBMED   10064593
REFERENCE   8  (bases 1 to 2343)
  AUTHORS   Chan,A.M., Miki,T., Meyers,K.A. and Aaronson,S.A.
  TITLE     A human oncogene of the RAS superfamily unmasked by expression cDNA
            cloning
  JOURNAL   Proc Natl Acad Sci U S A 91 (16), 7558-7562 (1994)
   PUBMED   8052619
REFERENCE   9  (bases 1 to 2343)
  AUTHORS   Roberts,A.E.
  TITLE     Noonan Syndrome
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301303
REFERENCE   10 (bases 1 to 2343)
  AUTHORS   Drivas,G.T., Shih,A., Coutavas,E., Rush,M.G. and D'Eustachio,P.
  TITLE     Characterization of four novel ras-like genes expressed in a human
            teratocarcinoma cell line
  JOURNAL   Mol Cell Biol 10 (4), 1793-1798 (1990)
   PUBMED   2108320
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK313976.1, AK309683.1,
            BQ023701.1, AA873016.1 and BQ020351.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_012250.5.
            
            Summary: This gene encodes a member of the R-Ras subfamily of
            Ras-like small GTPases. The encoded protein associates with the
            plasma membrane and may function as a signal transducer. This
            protein may play an important role in activating signal
            transduction pathways that control cell proliferation. Mutations in
            this gene are associated with the growth of certain tumors.
            Pseudogenes of this gene are found on chromosomes 1 and 2.
            Alternate splicing results in multiple transcript variants.
            [provided by RefSeq, Apr 2010].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1163655.333005.1,
                                           SRR1163658.249786.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000256196.9/ ENSP00000256196.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-928               AK313976.1         1-928
            929-1162            AK309683.1         737-970
            1163-1515           BQ023701.1         13-365              c
            1516-1927           AA873016.1         1-412               c
            1928-2343           BQ020351.1         17-432              c
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11p15.2"
     gene            1..2343
                     /gene="RRAS2"
                     /gene_synonym="NS12; TC21"
                     /note="RAS related 2"
                     /db_xref="GeneID:22800"
                     /db_xref="HGNC:HGNC:17271"
                     /db_xref="MIM:600098"
     exon            1..421
                     /gene="RRAS2"
                     /gene_synonym="NS12; TC21"
                     /inference="alignment:Splign:2.1.0"
     CDS             314..928
                     /gene="RRAS2"
                     /gene_synonym="NS12; TC21"
                     /EC_number="3.6.5.2"
                     /note="isoform a is encoded by transcript variant 1;
                     ras-like protein TC21; teratocarcinoma oncogene; related
                     RAS viral (r-ras) oncogene homolog 2"
                     /codon_start=1
                     /product="ras-related protein R-Ras2 isoform a"
                     /protein_id="NP_036382.2"
                     /db_xref="CCDS:CCDS7814.1"
                     /db_xref="GeneID:22800"
                     /db_xref="HGNC:HGNC:17271"
                     /db_xref="MIM:600098"
                     /translation="MAAAGWRDGSGQEKYRLVVVGGGGVGKSALTIQFIQSYFVTDYD
                     PTIEDSYTKQCVIDDRAARLDILDTAGQEEFGAMREQYMRTGEGFLLVFSVTDRGSFE
                     EIYKFQRQILRVKDRDEFPMILIGNKADLDHQRQVTQEEGQQLARQLKVTYMEASAKI
                     RMNVDQAFHELVRVIRKFQEQECPPSPEPTRKEKDKKGCHCVIF"
     exon            422..509
                     /gene="RRAS2"
                     /gene_synonym="NS12; TC21"
                     /inference="alignment:Splign:2.1.0"
     exon            510..612
                     /gene="RRAS2"
                     /gene_synonym="NS12; TC21"
                     /inference="alignment:Splign:2.1.0"
     exon            613..721
                     /gene="RRAS2"
                     /gene_synonym="NS12; TC21"
                     /inference="alignment:Splign:2.1.0"
     exon            722..840
                     /gene="RRAS2"
                     /gene_synonym="NS12; TC21"
                     /inference="alignment:Splign:2.1.0"
     exon            841..2343
                     /gene="RRAS2"
                     /gene_synonym="NS12; TC21"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1496..1501
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RRAS2"
                     /gene_synonym="NS12; TC21"
                     /note="hexamer: ATTAAA"
     polyA_site      1515
                     /gene="RRAS2"
                     /gene_synonym="NS12; TC21"
                     /note="major polyA site"
     regulatory      2320..2325
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RRAS2"
                     /gene_synonym="NS12; TC21"
                     /note="hexamer: ATTAAA"
     polyA_site      2343
                     /gene="RRAS2"
                     /gene_synonym="NS12; TC21"
                     /note="major polyA site"
ORIGIN      
        1 agacggccat ttgtggcggc gctggaggct gcgttcggca ggcgctgcgg agacgcgtag
       61 aggagcgcgc cccccggccg ctgccgcccc tggcccgtgc cgtcaccccg cttctccgcg
      121 cctcgggcgg tacccagcca gtccccagcg ccgcgctacc gcgctgaccg gccctccaga
      181 cgcctcccgg tacccgggac cccagcccgg ccgctcgccc gcagcccgcc ggccgcacac
      241 gtccccggag ccgggcctag ggcgggcggc agcggcggct cggcgcagtc aggctgggct
      301 ctgtagcgtc cccatggccg cggccggctg gcgggacggc tccggccagg agaagtaccg
      361 gctcgtggtg gtcggcgggg gcggcgtggg caagtcggcg ctcaccatcc agttcatcca
      421 gtcctatttt gtaacggatt atgatccaac cattgaagat tcttacacaa agcagtgtgt
      481 gatagatgac agagcagccc ggctagatat tttggataca gcaggacaag aagagtttgg
      541 agccatgaga gaacagtata tgaggactgg cgaaggcttc ctgttggtct tttcagtcac
      601 agatagaggc agttttgaag aaatctataa gtttcaaaga cagattctca gagtaaagga
      661 tcgtgatgag ttcccaatga ttttaattgg taataaagca gatctggatc atcaaagaca
      721 ggtaacacag gaagaaggac aacagttagc acggcagctt aaggtaacat acatggaggc
      781 atcagcaaag attaggatga atgtagatca agctttccat gaacttgtcc gggttatcag
      841 gaaatttcaa gagcaggaat gtcctccttc accagaacca acacggaaag aaaaagacaa
      901 gaaaggctgc cattgtgtca ttttctagaa tcccttcagt tttagctacc aacggccagg
      961 aaaagccctc atcttctctt tctctcctca gtttacatct tgttggtacc tttctagcct
     1021 tagacaaatg atcaccatgt tagccttaga cgaagaagct ggctagtcct ttctgtgaag
     1081 ctaatacaat ggtcatttcc agacaaattt aaaggaaaca ctaaggctgc ttcaaagatt
     1141 atctgattcc tttaaaatat atgtctatat acacagacat gctctttttt taagtgctta
     1201 cattttaata gagatgaatc agttttggaa tctaagctgt ttgccaagct gaagctacag
     1261 gttgtgaaat aatttttaac ttttggaatc atactgccta ctgttactct aaatagaaat
     1321 atagggtttt ttttaatgtg aatttttgcc tatctttaaa catttcaatg tcagcctttg
     1381 ttaaccttaa atacactgaa ttgaatctac aaaagtgaac catctcagac ctttactgat
     1441 actacaactt ttgttttctg atggccaaaa taccaaatgc ctgttgtatt tatggattaa
     1501 aaactgctta taaaaccctg tgttactact cctactcttg gagatgataa tattctatgt
     1561 ggtcaaatat ttggactcat ttaggactta gatatttcag tgtacttgat tttttaattt
     1621 aactcttttt cacagccacg ctaagggtaa aaaggaataa tttccttctg tcttcctttt
     1681 caagtatttc tgggtaaggg attcaaaaaa ctaaaactgt ttttgtttgt aatataaaat
     1741 atggaattga tctttccagg gtcagagatg attaatgttt ttgctatata cttttataca
     1801 ttattttctt atcaaactag ttaacaagta tttttatatg tttgtaagca gatatgcttt
     1861 catagcatac cttgtgtata tgtaaagata agtatttaat tctcactgtt cacttttaac
     1921 tgacaaagaa aaacaagtgg aaactacaga aactgtggta gaacttttac ttgctggtct
     1981 ggtcttggtt gtacccatct ttggccagtc acataactac tcaagaaacc ttcccaatag
     2041 agtacaacag gatgagactc tgaaatcact ttcagtattc cctgctagat attgattgtt
     2101 atttcaagta ttaagtgtaa gcttttaatg gataattagt ataactgtgg atggcatctg
     2161 attttgtttt taattctgtg gattgtgttt aagcaattca atagtatgtt cctgattttg
     2221 agatgctaag tggtattgca cagttgtcac tttatcaagt gtgtacaaca gtcccatgaa
     2281 gtttatagag catacccttg tatagcttca ggtgctagaa ttaaaattga tctgttatca
     2341 caa
//
