LOCUS       NM_004165               1460 bp    mRNA    linear   PRI 01-JUN-2024
DEFINITION  Homo sapiens RRAD, Ras related glycolysis inhibitor and calcium
            channel regulator (RRAD), transcript variant 2, mRNA.
ACCESSION   NM_004165
VERSION     NM_004165.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Sun,Z., Li,Y., Tan,X., Liu,W., He,X., Pan,D., Li,E., Xu,L. and
            Long,L.
  TITLE     Friend or Foe: Regulation, Downstream Effectors of RRAD in Cancer
  JOURNAL   Biomolecules 13 (3), 477 (2023)
   PUBMED   36979412
  REMARK    GeneRIF: Friend or Foe: Regulation, Downstream Effectors of RRAD in
            Cancer.
            Review article
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1460)
  AUTHORS   Katz,M., Subramaniam,S., Chomsky-Hecht,O., Tsemakhovich,V.,
            Flockerzi,V., Klussmann,E., Hirsch,J.A., Weiss,S. and Dascal,N.
  TITLE     Reconstitution of beta-adrenergic regulation of CaV1.2:
            Rad-dependent and Rad-independent protein kinase A mechanisms
  JOURNAL   Proc Natl Acad Sci U S A 118 (21) (2021)
   PUBMED   34001616
  REMARK    GeneRIF: Reconstitution of beta-adrenergic regulation of CaV1.2:
            Rad-dependent and Rad-independent protein kinase A mechanisms.
REFERENCE   3  (bases 1 to 1460)
  AUTHORS   Kim,H.K., Lee,I., Kim,S.T., Lee,J., Kim,K.M., Park,J.O. and
            Kang,W.K.
  TITLE     RRAD expression in gastric and colorectal cancer with peritoneal
            carcinomatosis
  JOURNAL   Sci Rep 9 (1), 19439 (2019)
   PUBMED   31857616
  REMARK    GeneRIF: RRAD expression in gastric and colorectal cancer with
            peritoneal carcinomatosis.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1460)
  AUTHORS   Belbachir,N., Portero,V., Al Sayed,Z.R., Gourraud,J.B.,
            Dilasser,F., Jesel,L., Guo,H., Wu,H., Gaborit,N., Guilluy,C.,
            Girardeau,A., Bonnaud,S., Simonet,F., Karakachoff,M., Pattier,S.,
            Scott,C., Burel,S., Marionneau,C., Chariau,C., Gaignerie,A.,
            David,L., Genin,E., Deleuze,J.F., Dina,C., Sauzeau,V., Loirand,G.,
            Baro,I., Schott,J.J., Probst,V., Wu,J.C., Redon,R., Charpentier,F.
            and Le Scouarnec,S.
  TITLE     RRAD mutation causes electrical and cytoskeletal defects in
            cardiomyocytes derived from a familial case of Brugada syndrome
  JOURNAL   Eur Heart J 40 (37), 3081-3094 (2019)
   PUBMED   31114854
  REMARK    GeneRIF: RRAD mutation causes electrical and cytoskeletal defects
            in cardiomyocytes derived from a familial case of Brugada syndrome.
REFERENCE   5  (bases 1 to 1460)
  AUTHORS   Wei,Z., Guo,H., Qin,J., Lu,S., Liu,Q., Zhang,X., Zou,Y., Gong,Y.
            and Shao,C.
  TITLE     Pan-senescence transcriptome analysis identified RRAD as a marker
            and negative regulator of cellular senescence
  JOURNAL   Free Radic Biol Med 130, 267-277 (2019)
   PUBMED   30391675
  REMARK    GeneRIF: this study established RRAD to be a biomarker as well as a
            novel negative regulator of cellular senescence.
REFERENCE   6  (bases 1 to 1460)
  AUTHORS   Caldwell,J.S., Moyers,J.S., Doria,A., Reynet,C. and Kahn,R.C.
  TITLE     Molecular cloning of the human rad gene: gene structure and
            complete nucleotide sequence
  JOURNAL   Biochim Biophys Acta 1316 (3), 145-148 (1996)
   PUBMED   8781531
REFERENCE   7  (bases 1 to 1460)
  AUTHORS   Zhu,J., Bilan,P.J., Moyers,J.S., Antonetti,D.A. and Kahn,C.R.
  TITLE     Rad, a novel Ras-related GTPase, interacts with skeletal muscle
            beta-tropomyosin
  JOURNAL   J Biol Chem 271 (2), 768-773 (1996)
   PUBMED   8557685
REFERENCE   8  (bases 1 to 1460)
  AUTHORS   Zhu,J., Reynet,C., Caldwell,J.S. and Kahn,C.R.
  TITLE     Characterization of Rad, a new member of Ras/GTPase superfamily,
            and its regulation by a unique GTPase-activating protein (GAP)-like
            activity
  JOURNAL   J Biol Chem 270 (9), 4805-4812 (1995)
   PUBMED   7876254
REFERENCE   9  (bases 1 to 1460)
  AUTHORS   Doria,A., Caldwell,J.S., Ji,L., Reynet,C., Rich,S.S.,
            Weremowicz,S., Morton,C.C., Warram,J.H., Kahn,C.R. and
            Krolewski,A.S.
  TITLE     Trinucleotide repeats at the rad locus. Allele distributions in
            NIDDM and mapping to a 3-cM region on chromosome 16q
  JOURNAL   Diabetes 44 (2), 243-247 (1995)
   PUBMED   7859947
REFERENCE   10 (bases 1 to 1460)
  AUTHORS   Reynet,C. and Kahn,C.R.
  TITLE     Rad: a member of the Ras family overexpressed in muscle of type II
            diabetic humans
  JOURNAL   Science 262 (5138), 1441-1444 (1993)
   PUBMED   8248782
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BP257026.1, L24564.1 and N93445.1.
            
            On Nov 23, 2018 this sequence version replaced NM_004165.2.
            
            Transcript Variant: This variant (2) uses an alternate splice
            junction in the 5' UTR compared to variant 1. Variants 1 and 2 both
            encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC011645.2, ERR279839.8212.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000299759.11/ ENSP00000299759.6
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-19                BP257026.1         2-20
            20-1416             L24564.1           1-1397
            1417-1460           N93445.1           9-52                c
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16q22.1"
     gene            1..1460
                     /gene="RRAD"
                     /gene_synonym="RAD; RAD1; REM3"
                     /note="RRAD, Ras related glycolysis inhibitor and calcium
                     channel regulator"
                     /db_xref="GeneID:6236"
                     /db_xref="HGNC:HGNC:10446"
                     /db_xref="MIM:179503"
     exon            1..127
                     /gene="RRAD"
                     /gene_synonym="RAD; RAD1; REM3"
                     /inference="alignment:Splign:2.1.0"
     exon            128..512
                     /gene="RRAD"
                     /gene_synonym="RAD; RAD1; REM3"
                     /inference="alignment:Splign:2.1.0"
     CDS             143..1069
                     /gene="RRAD"
                     /gene_synonym="RAD; RAD1; REM3"
                     /EC_number="3.6.5.2"
                     /note="RAS (RAD and GEM) like GTP binding 3; Ras-related
                     associated with diabetes; RRAD, Ras related glycolysis
                     inihibitor and calcium channel regulator; ras associated
                     with diabetes"
                     /codon_start=1
                     /product="GTP-binding protein RAD"
                     /protein_id="NP_004156.1"
                     /db_xref="CCDS:CCDS10824.1"
                     /db_xref="GeneID:6236"
                     /db_xref="HGNC:HGNC:10446"
                     /db_xref="MIM:179503"
                     /translation="MTLNGGGSGAGGSRGGGQERERRRGSTPWGPAPPLHRRSMPVDE
                     RDLQAALTPGALTAAAAGTGTQGPRLDWPEDSEDSLSSGGSDSDESVYKVLLLGAPGV
                     GKSALARIFGGVEDGPEAEAAGHTYDRSIVVDGEEASLMVYDIWEQDGGRWLPGHCMA
                     MGDAYVIVYSVTDKGSFEKASELRVQLRRARQTDDVPIILVGNKSDLVRSREVSVDEG
                     RACAVVFDCKFIETSAALHHNVQALFEGVVRQIRLRRDSKEANARRQAGTRRRESLGK
                     KAKRFLGRIVARNSRKMAFRAKSKSCHDLSVL"
     exon            513..586
                     /gene="RRAD"
                     /gene_synonym="RAD; RAD1; REM3"
                     /inference="alignment:Splign:2.1.0"
     exon            587..791
                     /gene="RRAD"
                     /gene_synonym="RAD; RAD1; REM3"
                     /inference="alignment:Splign:2.1.0"
     exon            792..1460
                     /gene="RRAD"
                     /gene_synonym="RAD; RAD1; REM3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1440..1445
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RRAD"
                     /gene_synonym="RAD; RAD1; REM3"
                     /note="hexamer: AATAAA"
     polyA_site      1460
                     /gene="RRAD"
                     /gene_synonym="RAD; RAD1; REM3"
                     /note="major polyA site"
ORIGIN      
        1 agtgcgggct gagaaggcgg tggctgcagc agcagcggcg gcggaaaccc taaagtccga
       61 gtccggacta cgagtgcgtg gcctcctaat ccggatccta gtcctgagcg tgtctgtgtg
      121 cgagtggacg gtcccggacg cgatgaccct gaacggcggc ggcagcggag cgggcgggag
      181 ccgcggtggg ggccaggagc gcgagcgccg tcggggcagc acaccctggg gccccgcccc
      241 gccgctgcac cgccgcagca tgccggtgga cgagcgcgac ctgcaggcgg cgctgacccc
      301 gggtgccctg acggcggccg cggccgggac ggggacccag ggtcccaggc tggactggcc
      361 cgaggactcc gaggactcgc tcagctcagg gggcagcgac tcagacgaga gcgtttacaa
      421 ggtgctgctg ctgggggcgc ccggcgtggg caagagcgcc ctggcgcgca tcttcggcgg
      481 tgtggaggac gggcctgaag cagaggcagc agggcacacc tatgatcgct ccattgtagt
      541 ggacggagaa gaggcatcac tcatggtcta cgacatttgg gagcaggacg ggggccgctg
      601 gttgcccggc cactgcatgg ccatggggga tgcctatgtc attgtgtact cagtgacgga
      661 caagggcagc ttcgagaagg cctcagaact gcgggtccag ctgcggcgtg cacggcaaac
      721 agatgatgtg cccatcatcc tcgtgggcaa caagagcgac ctggtgcgct ctcgtgaggt
      781 ctcggtggat gagggccggg cctgcgcggt ggtctttgac tgcaagttca ttgagacatc
      841 agcggcattg caccacaatg tccaggcgct gtttgaaggt gtcgtgcgcc agatacgcct
      901 gcgcagggac agcaaagaag ccaacgcacg acggcaagca ggcacccgga ggcgagagag
      961 ccttggcaaa aaggcgaagc gcttcttggg ccgcatcgta gctcgtaaca gccgcaagat
     1021 ggcctttcgc gccaaatcca agtcctgcca cgacctctcg gttctctagg tcccacccgc
     1081 tcccactatg gtgggagacg aacggaaggg ttggtgggct ggcccagcca actgccccgg
     1141 gtgcctcaga gcaggctcag actctgggtc cctcggagct gccagccggg cacccccaac
     1201 ctcatggtca tggacagata gacagtgctg ccctgcgaag tggctctcag gggccagtga
     1261 gggctgggcc cacagagatg catgcgcagg ctcatatgcg tcccaagcag ccgcagcgca
     1321 gccgccgggc aggcctgcgt gccgggagag gactctgcct tttttcacag cccgggtgtg
     1381 cctgccctgg agggaggctc ttcagtgcgg tagctatttg tttacatgca gatttttgta
     1441 ataaaggcta tttcctgata
//
