LOCUS       NM_152260               2365 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens RNA pseudouridine synthase domain containing 2
            (RPUSD2), transcript variant 1, mRNA.
ACCESSION   NM_152260
VERSION     NM_152260.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Martinez,N.M., Su,A., Burns,M.C., Nussbacher,J.K., Schaening,C.,
            Sathe,S., Yeo,G.W. and Gilbert,W.V.
  TITLE     Pseudouridine synthases modify human pre-mRNA co-transcriptionally
            and affect pre-mRNA processing
  JOURNAL   Mol Cell 82 (3), 645-659 (2022)
   PUBMED   35051350
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Carlile,T.M., Martinez,N.M., Schaening,C., Su,A., Bell,T.A.,
            Zinshteyn,B. and Gilbert,W.V.
  TITLE     mRNA structure determines modification by pseudouridine synthase 1
  JOURNAL   Nat Chem Biol 15 (10), 966-974 (2019)
   PUBMED   31477916
REFERENCE   3  (bases 1 to 2365)
  AUTHORS   Castello,A., Fischer,B., Eichelbaum,K., Horos,R., Beckmann,B.M.,
            Strein,C., Davey,N.E., Humphreys,D.T., Preiss,T., Steinmetz,L.M.,
            Krijgsveld,J. and Hentze,M.W.
  TITLE     Insights into RNA biology from an atlas of mammalian mRNA-binding
            proteins
  JOURNAL   Cell 149 (6), 1393-1406 (2012)
   PUBMED   22658674
REFERENCE   4  (bases 1 to 2365)
  AUTHORS   Wu,C., Ma,M.H., Brown,K.R., Geisler,M., Li,L., Tzeng,E., Jia,C.Y.,
            Jurisica,I. and Li,S.S.
  TITLE     Systematic identification of SH3 domain-mediated human
            protein-protein interactions by peptide array target screening
  JOURNAL   Proteomics 7 (11), 1775-1785 (2007)
   PUBMED   17474147
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DA746074.1, AK055971.1, BC016967.1 and AC091045.3.
            
            On Nov 23, 2018 this sequence version replaced NM_152260.2.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK301383.1, SRR1660807.208397.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000315616.12/ ENSP00000323288.7
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-39                DA746074.1         7-45
            40-800              AK055971.1         1-761
            801-2122            BC016967.1         768-2089
            2123-2365           AC091045.3         153912-154154
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q15.1"
     gene            1..2365
                     /gene="RPUSD2"
                     /gene_synonym="C15orf19; C18B11; PUS9"
                     /note="RNA pseudouridine synthase domain containing 2"
                     /db_xref="GeneID:27079"
                     /db_xref="HGNC:HGNC:24180"
     exon            1..645
                     /gene="RPUSD2"
                     /gene_synonym="C15orf19; C18B11; PUS9"
                     /inference="alignment:Splign:2.1.0"
     CDS             40..1677
                     /gene="RPUSD2"
                     /gene_synonym="C15orf19; C18B11; PUS9"
                     /note="isoform 1 is encoded by transcript variant 1;
                     C18B11 homolog (44.9kD); RNA pseudouridylate synthase
                     domain containing 2; RNA pseudouridylate synthase
                     domain-containing protein 2; pseudouridylate synthase
                     RPUSD2"
                     /codon_start=1
                     /product="pseudouridylate synthase RPUSD2 isoform 1"
                     /protein_id="NP_689473.1"
                     /db_xref="CCDS:CCDS10061.1"
                     /db_xref="GeneID:27079"
                     /db_xref="HGNC:HGNC:24180"
                     /translation="MWLDRRGWLRVLGHWRYDLRRPSFTRTWSGDKGPMAETVSTQVG
                     TEGGLRASHQQNGDAGGDAKVELSPGPPKPAGREVEPAPVGGEHPSAAAPGPGKHKKR
                     RGATRERVVPPPKKRRTGVSFGDEHFAETSYYFEGGLRKVRPYYFDFRTYCKGRWVGH
                     SLLHVFSTEFRAQPLAYYEAAVRAGRLQLNEKPVQDLNIVLKDNDFLRNTVHRHEPPV
                     TAEPIRLLAENEDVVVVDKPSSIPVHPCGRFRHNTVIFILGKEHQLKELHPLHRLDRL
                     TSGVLMFAKTAAVSERIHEQVRDRQLEKEYVCRVEGEFPTEEVTCKEPILVVSYKVGV
                     CRVDPRGKPCETVFQRLSYNGQSSVVRCRPLTGRTHQIRVHLQFLGHPILNDPIYNSV
                     AWGPSRGRGGYIPKTNEELLRDLVAEHQAKQSLDVLDLCEGDLSPGLTDSTAPSSELG
                     KDDLEELAAAAQKMEEVAEAAPQELDTIALASEKAVETDVMNQETDPLCAECRLVRQD
                     PLPQDLVMFLHALRYKGPGFEYFSPMPAWAQDDWQKD"
     exon            646..942
                     /gene="RPUSD2"
                     /gene_synonym="C15orf19; C18B11; PUS9"
                     /inference="alignment:Splign:2.1.0"
     exon            943..2365
                     /gene="RPUSD2"
                     /gene_synonym="C15orf19; C18B11; PUS9"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1856..1861
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RPUSD2"
                     /gene_synonym="C15orf19; C18B11; PUS9"
                     /note="hexamer: AATATA"
     polyA_site      1878
                     /gene="RPUSD2"
                     /gene_synonym="C15orf19; C18B11; PUS9"
                     /note="major polyA site"
     regulatory      2340..2345
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RPUSD2"
                     /gene_synonym="C15orf19; C18B11; PUS9"
                     /note="hexamer: TATAAA"
     polyA_site      2365
                     /gene="RPUSD2"
                     /gene_synonym="C15orf19; C18B11; PUS9"
ORIGIN      
        1 agatcgcgac cctgggagtg gagtgggggc agcgtggtta tgtggctgga ccgccgcgga
       61 tggctcaggg ttcttggaca ttggcgctac gaccttaggc gccctagctt taccaggact
      121 tggagtggcg ataagggccc aatggcagaa acagtgtcta cccaggttgg gacagagggc
      181 gggctgaggg cttcgcatca gcaaaacggt gacgctggtg gcgacgcgaa ggttgagctg
      241 tcccccgggc ccccgaagcc ggctggccgg gaagtggagc cggccccagt aggcggggag
      301 catccctcgg ctgcagcccc aggcccgggc aagcataaga agcggcgggg cgcaaccagg
      361 gagcgtgtcg tgccgccccc gaagaagcgg cggaccgggg tgagcttcgg agatgagcac
      421 tttgcagaaa ccagttatta cttcgagggc ggcctgcgta aggtgcggcc ctattacttt
      481 gacttccgga cctactgcaa aggtcgctgg gtgggccaca gcttgctgca cgtcttcagt
      541 accgagttcc gagctcagcc cctggcctac tatgaggccg cggtccgggc gggccgcctg
      601 caactcaacg agaagccggt gcaggacctc aacatcgtgc tcaaggacaa tgatttcttg
      661 cggaacacag tgcacaggca tgagccacca gtcacagcag agcccattcg cctgctagct
      721 gagaacgaag atgtggtggt tgtagacaag ccttcctcca ttcccgttca cccctgtggc
      781 cgcttccgac acaacacagt tatcttcatc ctaggcaagg agcaccaact caaggagcta
      841 caccccttgc atcggcttga ccgccttacc tcaggggtgc ttatgtttgc caagacagct
      901 gcagtctctg agagaattca cgagcaggtt cgggaccggc agctggagaa ggagtacgtg
      961 tgccgggtgg aaggggagtt ccccactgag gaagtgacct gtaaagaacc catcttagtg
     1021 gtgtcttaca aagtaggggt gtgccgtgta gatccccggg gcaagccctg tgagacagtg
     1081 ttccagaggc taagctacaa tggccagtcc agtgtggtac ggtgccggcc actcacaggc
     1141 cgcacacacc agattcgagt ccaccttcag ttcttgggcc atcccattct caacgacccc
     1201 atctacaact cagttgcctg gggtccttct cgaggccggg gcggctacat tcccaagaca
     1261 aacgaggagt tgctacggga cctggtagca gagcaccagg ccaaacagag cctggatgtg
     1321 ctagatctct gtgagggtga cctgtcccca ggactcacag actctacggc cccctcctca
     1381 gagttgggca aggacgacct ggaagagttg gctgcagctg cccagaagat ggaggaagta
     1441 gctgaggcag cccctcagga gttggacaca atagccttgg catcagagaa ggcagttgaa
     1501 acagatgtca tgaatcaaga gacagaccca ctctgtgcag agtgccggct ggtgcgacag
     1561 gatcccttgc cccaagacct tgtgatgttc ctacatgccc tacgctataa agggccaggc
     1621 tttgagtact tttcaccaat gcctgcctgg gcacaggatg actggcaaaa ggactgaggg
     1681 tgtggccaat ggagggattg cttcttgggt tgtgacaagg atgggctata gggcaagggc
     1741 tgaccccatg ggctagtact tggggtttct ataggaatga ggacgggctt ctaaagagac
     1801 ctgctcatac ttgctacctc cttccagtgg gaatttggag actttttggt ttgtaaatat
     1861 atcccttttt ctaacatctt tgatgtctgg ttttcttccg gcttcttttt tattactgat
     1921 gtaaaattca cataacataa aattaaccac tttaactagc caggcacagt ggctcatggc
     1981 tataatccta gtactttggg aggtagaagt gaaaggatca ctggaggcca agagttcaag
     2041 gttaaagtga actgtgattg tgctgctgca ctccagagcc taggtgacag agtgagaccc
     2101 tgtctcttaa aaaaaaaaaa aattaaccaa tttaagatgt acagcgtagt cacatttagt
     2161 acattcacag tgttgtacaa ccataacctc atatatttcc aaaacatttt catcacccca
     2221 aaaggaaacc tgtacccatt aagtaagtgg tcactttcca ctccccttcc accagcctct
     2281 ggcaaccact aatctacttt ctgtctgttt ggatttgcct gtttattctg gacattttat
     2341 ataaatggaa tgataaaaca tgtga
//
