LOCUS       NM_003161               5428 bp    mRNA    linear   PRI 22-MAR-2025
DEFINITION  Homo sapiens ribosomal protein S6 kinase B1 (RPS6KB1), transcript
            variant 1, mRNA.
ACCESSION   NM_003161
VERSION     NM_003161.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5428)
  AUTHORS   Huang,Y., Yue,S., Yan,Z., Liu,Y., Qiao,J., Zhang,M., Dong,Y. and
            Zheng,J.
  TITLE     Lactate-upregulated ARG2 expression induces cellular senescence in
            fibroblast-like synoviocytes of osteoarthritis via activating the
            mTOR/S6K1 signaling pathway
  JOURNAL   Int Immunopharmacol 142 (Pt A), 113071 (2024)
   PUBMED   39236462
  REMARK    GeneRIF: Lactate-upregulated ARG2 expression induces cellular
            senescence in fibroblast-like synoviocytes of osteoarthritis via
            activating the mTOR/S6K1 signaling pathway.
REFERENCE   2  (bases 1 to 5428)
  AUTHORS   Ru,J., Lu,J., Ge,J., Ding,B., Su,R., Jiang,Y., Sun,Y., Ma,J.,
            Li,Y., Sun,J., Xu,G., Tong,R., Zheng,S., Yang,B. and Wu,J.
  TITLE     IRGM is a novel regulator of PD-L1 via promoting S6K1-mediated
            phosphorylation of YBX1 in hepatocellular carcinoma
  JOURNAL   Cancer Lett 581, 216495 (2024)
   PUBMED   37993085
  REMARK    GeneRIF: IRGM is a novel regulator of PD-L1 via promoting
            S6K1-mediated phosphorylation of YBX1 in hepatocellular carcinoma.
REFERENCE   3  (bases 1 to 5428)
  AUTHORS   Ben-Hur,V., Denichenko,P., Siegfried,Z., Maimon,A., Krainer,A.,
            Davidson,B. and Karni,R.
  TITLE     S6K1 alternative splicing modulates its oncogenic activity and
            regulates mTORC1
  JOURNAL   Cell Rep 3 (1), 103-115 (2013)
   PUBMED   23273915
  REMARK    GeneRIF: alternative splicing of S6K1 acts as a molecular switch in
            breast cancer cells, elevating oncogenic isoforms that activate
            mTORC1.
REFERENCE   4  (bases 1 to 5428)
  AUTHORS   Rosner,M. and Hengstschlager,M.
  TITLE     Nucleocytoplasmic localization of p70 S6K1, but not of its isoforms
            p85 and p31, is regulated by TSC2/mTOR
  JOURNAL   Oncogene 30 (44), 4509-4522 (2011)
   PUBMED   21602892
REFERENCE   5  (bases 1 to 5428)
  AUTHORS   Fenton,T.R. and Gout,I.T.
  TITLE     Functions and regulation of the 70kDa ribosomal S6 kinases
  JOURNAL   Int J Biochem Cell Biol 43 (1), 47-59 (2011)
   PUBMED   20932932
  REMARK    GeneRIF: Dysregulation of S6 kinases contributes to the
            pathogenesis of human diseases.
            Review article
REFERENCE   6  (bases 1 to 5428)
  AUTHORS   Karni,R., de Stanchina,E., Lowe,S.W., Sinha,R., Mu,D. and
            Krainer,A.R.
  TITLE     The gene encoding the splicing factor SF2/ASF is a proto-oncogene
  JOURNAL   Nat Struct Mol Biol 14 (3), 185-193 (2007)
   PUBMED   17310252
REFERENCE   7  (bases 1 to 5428)
  AUTHORS   Couch,F.J., Wang,X.Y., Wu,G.J., Qian,J., Jenkins,R.B. and
            James,C.D.
  TITLE     Localization of PS6K to chromosomal region 17q23 and determination
            of its amplification in breast cancer
  JOURNAL   Cancer Res 59 (7), 1408-1411 (1999)
   PUBMED   10197603
REFERENCE   8  (bases 1 to 5428)
  AUTHORS   Moser,B.A., Dennis,P.B., Pullen,N., Pearson,R.B., Williamson,N.A.,
            Wettenhall,R.E., Kozma,S.C. and Thomas,G.
  TITLE     Dual requirement for a newly identified phosphorylation site in
            p70s6k
  JOURNAL   Mol Cell Biol 17 (9), 5648-5655 (1997)
   PUBMED   9271440
REFERENCE   9  (bases 1 to 5428)
  AUTHORS   Proud,C.G.
  TITLE     p70 S6 kinase: an enigma with variations
  JOURNAL   Trends Biochem Sci 21 (5), 181-185 (1996)
   PUBMED   8871403
  REMARK    Review article
REFERENCE   10 (bases 1 to 5428)
  AUTHORS   Grove,J.R., Banerjee,P., Balasubramanyam,A., Coffer,P.J.,
            Price,D.J., Avruch,J. and Woodgett,J.R.
  TITLE     Cloning and expression of two human p70 S6 kinase polypeptides
            differing only at their amino termini
  JOURNAL   Mol Cell Biol 11 (11), 5541-5550 (1991)
   PUBMED   1922062
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BP359158.1, AK312875.1,
            AC004686.2, AK297147.1 and CA444907.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_003161.3.
            
            Summary: This gene encodes a member of the ribosomal S6 kinase
            family of serine/threonine kinases. The encoded protein responds to
            mTOR (mammalian target of rapamycin) signaling to promote protein
            synthesis, cell growth, and cell proliferation. Activity of this
            gene has been associated with human cancer. Alternatively spliced
            transcript variants have been observed. The use of alternative
            translation start sites results in isoforms with longer or shorter
            N-termini which may differ in their subcellular localizations.
            There are two pseudogenes for this gene on chromosome 17. [provided
            by RefSeq, Jan 2013].
            
            Transcript Variant: This variant (1) can initiate translation from
            two alternate in-frame AUG start codons. The isoform represented in
            this RefSeq (a, also known as p85 or alpha I) is derived from the
            first AUG start codon, and is the longest isoform.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803615.266284.1,
                                           SRR1803612.241251.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000225577.9/ ENSP00000225577.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-19                BP359158.1         76-94
            20-535              AK312875.1         1-516
            536-536             AC004686.2         28745-28745         c
            537-1345            AK312875.1         518-1326
            1346-2035           AK297147.1         1357-2046
            2036-4843           AC004686.2         5281-8088           c
            4844-5280           CA444907.1         14-450              c
            5281-5428           AC004686.2         4696-4843           c
FEATURES             Location/Qualifiers
     source          1..5428
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q23.1"
     gene            1..5428
                     /gene="RPS6KB1"
                     /gene_synonym="p70 S6KA; p70(S6K)-alpha; p70-alpha;
                     p70-S6K; PS6K; S6K; S6K-beta-1; S6K1; STK14A"
                     /note="ribosomal protein S6 kinase B1"
                     /db_xref="GeneID:6198"
                     /db_xref="HGNC:HGNC:10436"
                     /db_xref="MIM:608938"
     exon            1..205
                     /gene="RPS6KB1"
                     /gene_synonym="p70 S6KA; p70(S6K)-alpha; p70-alpha;
                     p70-S6K; PS6K; S6K; S6K-beta-1; S6K1; STK14A"
                     /inference="alignment:Splign:2.1.0"
     CDS             65..1642
                     /gene="RPS6KB1"
                     /gene_synonym="p70 S6KA; p70(S6K)-alpha; p70-alpha;
                     p70-S6K; PS6K; S6K; S6K-beta-1; S6K1; STK14A"
                     /EC_number="2.7.11.1"
                     /note="p85, alpha I; isoform a is encoded by transcript
                     variant 1; serine/threonine kinase 14 alpha; ribosomal
                     protein S6 kinase beta-1; ribosomal protein S6 kinase I;
                     serine/threonine-protein kinase 14A; ribosomal protein S6
                     kinase, 70kDa, polypeptide 1"
                     /codon_start=1
                     /product="ribosomal protein S6 kinase beta-1 isoform a"
                     /protein_id="NP_003152.1"
                     /db_xref="CCDS:CCDS11621.1"
                     /db_xref="GeneID:6198"
                     /db_xref="HGNC:HGNC:10436"
                     /db_xref="MIM:608938"
                     /translation="MRRRRRRDGFYPAPDFRDREAEDMAGVFDIDLDQPEDAGSEDEL
                     EEGGQLNESMDHGGVGPYELGMEHCEKFEISETSVNRGPEKIRPECFELLRVLGKGGY
                     GKVFQVRKVTGANTGKIFAMKVLKKAMIVRNAKDTAHTKAERNILEEVKHPFIVDLIY
                     AFQTGGKLYLILEYLSGGELFMQLEREGIFMEDTACFYLAEISMALGHLHQKGIIYRD
                     LKPENIMLNHQGHVKLTDFGLCKESIHDGTVTHTFCGTIEYMAPEILMRSGHNRAVDW
                     WSLGALMYDMLTGAPPFTGENRKKTIDKILKCKLNLPPYLTQEARDLLKKLLKRNAAS
                     RLGAGPGDAGEVQAHPFFRHINWEELLARKVEPPFKPLLQSEEDVSQFDSKFTRQTPV
                     DSPDDSTLSESANQVFLGFTYVAPSVLESVKEKFSFEPKIRSPRRFIGSPRTPVSPVK
                     FSPGDFWGRGASASTANPQTPVEYPMETSGIEQMDVTMSGEASAPLPIRQPNSGPYKK
                     QAFPMISKRPEHLRMNL"
     exon            206..255
                     /gene="RPS6KB1"
                     /gene_synonym="p70 S6KA; p70(S6K)-alpha; p70-alpha;
                     p70-S6K; PS6K; S6K; S6K-beta-1; S6K1; STK14A"
                     /inference="alignment:Splign:2.1.0"
     exon            256..376
                     /gene="RPS6KB1"
                     /gene_synonym="p70 S6KA; p70(S6K)-alpha; p70-alpha;
                     p70-S6K; PS6K; S6K; S6K-beta-1; S6K1; STK14A"
                     /inference="alignment:Splign:2.1.0"
     exon            377..445
                     /gene="RPS6KB1"
                     /gene_synonym="p70 S6KA; p70(S6K)-alpha; p70-alpha;
                     p70-S6K; PS6K; S6K; S6K-beta-1; S6K1; STK14A"
                     /inference="alignment:Splign:2.1.0"
     exon            446..593
                     /gene="RPS6KB1"
                     /gene_synonym="p70 S6KA; p70(S6K)-alpha; p70-alpha;
                     p70-S6K; PS6K; S6K; S6K-beta-1; S6K1; STK14A"
                     /inference="alignment:Splign:2.1.0"
     exon            594..651
                     /gene="RPS6KB1"
                     /gene_synonym="p70 S6KA; p70(S6K)-alpha; p70-alpha;
                     p70-S6K; PS6K; S6K; S6K-beta-1; S6K1; STK14A"
                     /inference="alignment:Splign:2.1.0"
     exon            652..752
                     /gene="RPS6KB1"
                     /gene_synonym="p70 S6KA; p70(S6K)-alpha; p70-alpha;
                     p70-S6K; PS6K; S6K; S6K-beta-1; S6K1; STK14A"
                     /inference="alignment:Splign:2.1.0"
     exon            753..843
                     /gene="RPS6KB1"
                     /gene_synonym="p70 S6KA; p70(S6K)-alpha; p70-alpha;
                     p70-S6K; PS6K; S6K; S6K-beta-1; S6K1; STK14A"
                     /inference="alignment:Splign:2.1.0"
     exon            844..934
                     /gene="RPS6KB1"
                     /gene_synonym="p70 S6KA; p70(S6K)-alpha; p70-alpha;
                     p70-S6K; PS6K; S6K; S6K-beta-1; S6K1; STK14A"
                     /inference="alignment:Splign:2.1.0"
     exon            935..1042
                     /gene="RPS6KB1"
                     /gene_synonym="p70 S6KA; p70(S6K)-alpha; p70-alpha;
                     p70-S6K; PS6K; S6K; S6K-beta-1; S6K1; STK14A"
                     /inference="alignment:Splign:2.1.0"
     exon            1043..1105
                     /gene="RPS6KB1"
                     /gene_synonym="p70 S6KA; p70(S6K)-alpha; p70-alpha;
                     p70-S6K; PS6K; S6K; S6K-beta-1; S6K1; STK14A"
                     /inference="alignment:Splign:2.1.0"
     exon            1106..1183
                     /gene="RPS6KB1"
                     /gene_synonym="p70 S6KA; p70(S6K)-alpha; p70-alpha;
                     p70-S6K; PS6K; S6K; S6K-beta-1; S6K1; STK14A"
                     /inference="alignment:Splign:2.1.0"
     exon            1184..1291
                     /gene="RPS6KB1"
                     /gene_synonym="p70 S6KA; p70(S6K)-alpha; p70-alpha;
                     p70-S6K; PS6K; S6K; S6K-beta-1; S6K1; STK14A"
                     /inference="alignment:Splign:2.1.0"
     exon            1292..1404
                     /gene="RPS6KB1"
                     /gene_synonym="p70 S6KA; p70(S6K)-alpha; p70-alpha;
                     p70-S6K; PS6K; S6K; S6K-beta-1; S6K1; STK14A"
                     /inference="alignment:Splign:2.1.0"
     exon            1405..5428
                     /gene="RPS6KB1"
                     /gene_synonym="p70 S6KA; p70(S6K)-alpha; p70-alpha;
                     p70-S6K; PS6K; S6K; S6K-beta-1; S6K1; STK14A"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1751..1756
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RPS6KB1"
                     /gene_synonym="p70 S6KA; p70(S6K)-alpha; p70-alpha;
                     p70-S6K; PS6K; S6K; S6K-beta-1; S6K1; STK14A"
                     /note="hexamer: ATTACA"
     polyA_site      1781
                     /gene="RPS6KB1"
                     /gene_synonym="p70 S6KA; p70(S6K)-alpha; p70-alpha;
                     p70-S6K; PS6K; S6K; S6K-beta-1; S6K1; STK14A"
                     /note="major polyA site"
     regulatory      5253..5258
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RPS6KB1"
                     /gene_synonym="p70 S6KA; p70(S6K)-alpha; p70-alpha;
                     p70-S6K; PS6K; S6K; S6K-beta-1; S6K1; STK14A"
                     /note="hexamer: ATTAAA"
     polyA_site      5274
                     /gene="RPS6KB1"
                     /gene_synonym="p70 S6KA; p70(S6K)-alpha; p70-alpha;
                     p70-S6K; PS6K; S6K; S6K-beta-1; S6K1; STK14A"
                     /note="major polyA site"
     regulatory      5397..5402
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RPS6KB1"
                     /gene_synonym="p70 S6KA; p70(S6K)-alpha; p70-alpha;
                     p70-S6K; PS6K; S6K; S6K-beta-1; S6K1; STK14A"
                     /note="hexamer: AATAAA"
     polyA_site      5428
                     /gene="RPS6KB1"
                     /gene_synonym="p70 S6KA; p70(S6K)-alpha; p70-alpha;
                     p70-S6K; PS6K; S6K; S6K-beta-1; S6K1; STK14A"
ORIGIN      
        1 gcactgagcc taagcagccg gtgatggcgg cagcggctgt ggtggctgcg gcgggtccgg
       61 gcccatgagg cgacgaagga ggcgggacgg cttttaccca gccccggact tccgagacag
      121 ggaagctgag gacatggcag gagtgtttga catagacctg gaccagccag aggacgcggg
      181 ctctgaggat gagctggagg aggggggtca gttaaatgaa agcatggacc atgggggagt
      241 tggaccatat gaacttggca tggaacattg tgagaaattt gaaatctcag aaactagtgt
      301 gaacagaggg ccagaaaaaa tcagaccaga atgttttgag ctacttcggg tacttggtaa
      361 agggggctat ggaaaggttt ttcaagtacg aaaagtaaca ggagcaaata ctgggaaaat
      421 atttgccatg aaggtgctta aaaaggcaat gatagtaaga aatgctaaag atacagctca
      481 tacaaaagca gaacggaata ttctggagga agtaaagcat cccttcatcg tggatttaat
      541 ttatgccttt cagactggtg gaaaactcta cctcatcctt gagtatctca gtggaggaga
      601 actatttatg cagttagaaa gagagggaat atttatggaa gacactgcct gcttttactt
      661 ggcagaaatc tccatggctt tggggcattt acatcaaaag gggatcatct acagagacct
      721 gaagccggag aatatcatgc ttaatcacca aggtcatgtg aaactaacag actttggact
      781 atgcaaagaa tctattcatg atggaacagt cacacacaca ttttgtggaa caatagaata
      841 catggcccct gaaatcttga tgagaagtgg ccacaatcgt gctgtggatt ggtggagttt
      901 gggagcatta atgtatgaca tgctgactgg agcaccccca ttcactgggg agaatagaaa
      961 gaaaacaatt gacaaaatcc tcaaatgtaa actcaatttg cctccctacc tcacacaaga
     1021 agccagagat ctgcttaaaa agctgctgaa aagaaatgct gcttctcgtc tgggagctgg
     1081 tcctggggac gctggagaag ttcaagctca tccattcttt agacacatta actgggaaga
     1141 acttctggct cgaaaggtgg agcccccctt taaacctctg ttgcaatctg aagaggatgt
     1201 aagtcagttt gattccaagt ttacacgtca gacacctgtc gacagcccag atgactcaac
     1261 tctcagtgaa agtgccaatc aggtctttct gggttttaca tatgtggctc catctgtact
     1321 tgaaagtgtg aaagaaaagt tttcctttga accaaaaatc cgatcacctc gaagatttat
     1381 tggcagccca cgaacacctg tcagcccagt caaattttct cctggggatt tctggggaag
     1441 aggtgcttcg gccagcacag caaatcctca gacacctgtg gaatacccaa tggaaacaag
     1501 tggcatagag cagatggatg tgacaatgag tggggaagca tcggcaccac ttccaatacg
     1561 acagccgaac tctgggccat acaaaaaaca agcttttccc atgatctcca aacggccaga
     1621 gcacctgcgt atgaatctat gacagagcaa tgcttttaat gaatttaagg caaaaaaggt
     1681 ggagagggag atgtgtgagc atcctgcaag gtgaaacgac tcaaaatgac agtttcagag
     1741 agtcaatgtc attacataga acacttcaga cacaggaaaa ataaacgtgg attttaaaaa
     1801 atcaatcaat ggtgcaaaaa aaaacttaaa gcaaaatagt attgctgaac tcttaggcac
     1861 atcaattaat tgattcctcg cgacatcttc tcaaccttat caaggatttt catgttgatg
     1921 actcgaaact gacagtatta agggtaggat gttgcttctg aatcactgtt gagttctgat
     1981 tgtgttgaag aagggttatc ctttcattag gcaaagtaca aaattgccta taatacttgc
     2041 aactaaggac aaattagcat gcaagcttgg tcaaactttt tccagcaaaa tggaagcaaa
     2101 gacaaaagaa acttaccaat tgatgtttta cgtgcaaaca acctgaatct tttttttata
     2161 taaatatata tttttcaaat agatttttga ttcagctcat tatgaaaaac atcccaaact
     2221 ttaaaatgcg aaattattgg ttggtgtgaa gaaagccaga caacttctgt ttcttctctt
     2281 ggtgaaataa taaaatgcaa atgaatcatt gttaaccaca gctgtggctc gtttgaggga
     2341 ttggggtgga cctggggttt attttcagta acccagctgc aatacctgtc tgtaatatga
     2401 gaaaaaaaaa atgaatctat ttaatcattt ctacttgcag tactgctatg tgctaagctt
     2461 aactggaagc cttggaatgg gcataagttg tatgtcctac atttcatcat tgtcccgggc
     2521 ctgcattgca ctggaaaaaa aaatcgccac ctgttcttac accagtattt ggttcaagac
     2581 accaaatgtc ttcagcccat ggctgaagaa caacagaaga gagtcaggat aaaaaataca
     2641 tactgtggtc ggcaaggtga gggagatagg gatatccagg ggaagagggt gttgctgtgg
     2701 cccactctct gtctaatctc tttacagcaa attggtaaga ttttcagttt tacttctttc
     2761 tactgtttct gctgtctacc ttccttatat ttttttcctc aacagtttta aaaagaaaaa
     2821 aaggtctatt tttttttctc ctatacttgg gctacatttt ttgattgtaa aaatatttga
     2881 tggccttttg atgaatgtct tccacagtaa agaaaactta gtggcttaat ttaggaaaca
     2941 tgttaacagg acactatgtt tttgaaattg taacaaaatc tacataaatg atttacaggt
     3001 taaaagaata aaaataaagg taactttacc tttcttaaat atttcctgcc ttaaagagag
     3061 catttccatg actttagctg gtgaaagggt ttaatatctg cagagcttta taaaaatata
     3121 tttcagtgca tactggtata atagatgatc atgcagttgc agttgagttg tatcaccttt
     3181 tttgtttgtc ttttataatg tcttcagtct gagtgtgcaa agtcaatttg taatattttg
     3241 caaccctagg atttttttaa atagatgctg cttgctatgt tttcaaacct ttttgagcca
     3301 taggatccaa gccataaaat tctttatgca tgttgaattc agtcagaaaa gagcaaggct
     3361 ttgctttttg aaattgcaac tcaaatgaga tgggatgaaa tcctatgaca gtaagcaaaa
     3421 acagaaccat gaaaaatgat tggacataca ccttttcaat tgtggcaata attgaaagaa
     3481 tcgataaaag ttcatctttg gacagaaagc ctttaaaaaa aaaatcactc cctcttcccc
     3541 ctcctccctt attgcagcag cctactgaga actttgactg ttgctggtaa attagaagct
     3601 acaataataa ttaagggcag aaattatact taaaaagtgc agatccttgt tctttgacaa
     3661 tttgtgatgt ctgaaaaaac agaacccgaa aagctatggt gatatgtaca ggcattattt
     3721 cagactgtaa atggcttgtg atactcttga tacttgtttt caaatatgtt tactaactgt
     3781 agtgttgact gcctgaccaa attccagtga aacttataca ccaaaatatt cttcctaggt
     3841 cctatttgct agtaacatga gcactgtgat tggctggcta taaccacccc agttaaacca
     3901 ttttcataat tagtagtgcc agcaatagtg gcaaacactg caacttttct gcataaaaag
     3961 cattaattgc acagctacca tccacacaaa tacatagttt ttctgacttc acatttatta
     4021 agtgaaattt atttcccatg ctgtggaaag tttattgaga acttgtttca taaatggata
     4081 tccctactat gactgtgaaa acatgtcaag tgtcacatta gtgtcacaga cagaaagcac
     4141 acacctatgc aatatggctt atctatattt atttgtaaaa atccaagcat agtttaaaat
     4201 atgatgtcga tattactagt cttgagtttc taagagggtt ctttatgtta taccaggtaa
     4261 gtgtataaaa gagattaagt gctttttttt catcacttga ttattttctt taaaatcagc
     4321 tattacagga tattttttta ttttatacat gctgtttttt aattaaaata taatcactga
     4381 agtttactaa tttgatttta taaggtttgt agcattacag aataactaaa ctgggattta
     4441 taaaccagct gtgattaaca atgtaaagta ttaattattg aactttgaac cagattttta
     4501 ggaaaattat gttctttttc cccctttatg gtcttaacta atttgaatcc ttcaagaagg
     4561 atttttccat actatttttt aagatagaag ataatttgtg ggcaggggtg gaggatgcat
     4621 gtatgatact ccataaattc aacattcttt actataggta atgaatgatt ataaacaaga
     4681 tgcatcttag atagtattaa tatactgagc cttggattat atatttaata taggacctat
     4741 tttgaatatt cagttaatca tatggttcct agcttacaag ggctagatct aagattattc
     4801 ccatgagaaa tgttgaattt atgaagaata gattttaagg ctttgaaaat ggttaatttc
     4861 tcaaaaacat caatgtccaa acatctacct tttttcatag gagtagacac tagcaagctg
     4921 gacaaactat cacaaaagta tttgtcacac ataacctgtg gtctgttgct gattaataca
     4981 gtactttttc ttgtgtgatt cttaacatta tagcacaagt attatctcag tggattatcc
     5041 ggaataacat ctgaaagatg ggttcatcta tgtttgtgtt tgctctttaa actattgttt
     5101 ctcctatccc aagttcgctt tgcatctatc agtaaataaa attcttcagc tgccttatta
     5161 ggagtgctat gagggtaaca cctgttctgc ttttcatctt gtatttagtt gactgtatta
     5221 tttgatttcg gattgaatga atgtaaatag aaattaaatg caaatttgaa tgaacataaa
     5281 tagaagtgat ttattttttt attatcttaa agataggaag gaattagtat acgtatcagg
     5341 tgtataaatg gtttaaattt attttaacat gtcagatgtg ttcaagttgt catataaata
     5401 aactatgtat gtaaggaatg ggttaaaa
//
