LOCUS       NM_001369678            5503 bp    mRNA    linear   PRI 22-MAR-2025
DEFINITION  Homo sapiens ribosomal protein S6 kinase B1 (RPS6KB1), transcript
            variant 14, mRNA.
ACCESSION   NM_001369678 XM_017024930
VERSION     NM_001369678.1
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5503)
  AUTHORS   Huang,Y., Yue,S., Yan,Z., Liu,Y., Qiao,J., Zhang,M., Dong,Y. and
            Zheng,J.
  TITLE     Lactate-upregulated ARG2 expression induces cellular senescence in
            fibroblast-like synoviocytes of osteoarthritis via activating the
            mTOR/S6K1 signaling pathway
  JOURNAL   Int Immunopharmacol 142 (Pt A), 113071 (2024)
   PUBMED   39236462
  REMARK    GeneRIF: Lactate-upregulated ARG2 expression induces cellular
            senescence in fibroblast-like synoviocytes of osteoarthritis via
            activating the mTOR/S6K1 signaling pathway.
REFERENCE   2  (bases 1 to 5503)
  AUTHORS   Ru,J., Lu,J., Ge,J., Ding,B., Su,R., Jiang,Y., Sun,Y., Ma,J.,
            Li,Y., Sun,J., Xu,G., Tong,R., Zheng,S., Yang,B. and Wu,J.
  TITLE     IRGM is a novel regulator of PD-L1 via promoting S6K1-mediated
            phosphorylation of YBX1 in hepatocellular carcinoma
  JOURNAL   Cancer Lett 581, 216495 (2024)
   PUBMED   37993085
  REMARK    GeneRIF: IRGM is a novel regulator of PD-L1 via promoting
            S6K1-mediated phosphorylation of YBX1 in hepatocellular carcinoma.
REFERENCE   3  (bases 1 to 5503)
  AUTHORS   Ben-Hur,V., Denichenko,P., Siegfried,Z., Maimon,A., Krainer,A.,
            Davidson,B. and Karni,R.
  TITLE     S6K1 alternative splicing modulates its oncogenic activity and
            regulates mTORC1
  JOURNAL   Cell Rep 3 (1), 103-115 (2013)
   PUBMED   23273915
  REMARK    GeneRIF: alternative splicing of S6K1 acts as a molecular switch in
            breast cancer cells, elevating oncogenic isoforms that activate
            mTORC1.
REFERENCE   4  (bases 1 to 5503)
  AUTHORS   Rosner,M. and Hengstschlager,M.
  TITLE     Nucleocytoplasmic localization of p70 S6K1, but not of its isoforms
            p85 and p31, is regulated by TSC2/mTOR
  JOURNAL   Oncogene 30 (44), 4509-4522 (2011)
   PUBMED   21602892
REFERENCE   5  (bases 1 to 5503)
  AUTHORS   Fenton,T.R. and Gout,I.T.
  TITLE     Functions and regulation of the 70kDa ribosomal S6 kinases
  JOURNAL   Int J Biochem Cell Biol 43 (1), 47-59 (2011)
   PUBMED   20932932
  REMARK    GeneRIF: Dysregulation of S6 kinases contributes to the
            pathogenesis of human diseases.
            Review article
REFERENCE   6  (bases 1 to 5503)
  AUTHORS   Karni,R., de Stanchina,E., Lowe,S.W., Sinha,R., Mu,D. and
            Krainer,A.R.
  TITLE     The gene encoding the splicing factor SF2/ASF is a proto-oncogene
  JOURNAL   Nat Struct Mol Biol 14 (3), 185-193 (2007)
   PUBMED   17310252
REFERENCE   7  (bases 1 to 5503)
  AUTHORS   Couch,F.J., Wang,X.Y., Wu,G.J., Qian,J., Jenkins,R.B. and
            James,C.D.
  TITLE     Localization of PS6K to chromosomal region 17q23 and determination
            of its amplification in breast cancer
  JOURNAL   Cancer Res 59 (7), 1408-1411 (1999)
   PUBMED   10197603
REFERENCE   8  (bases 1 to 5503)
  AUTHORS   Moser,B.A., Dennis,P.B., Pullen,N., Pearson,R.B., Williamson,N.A.,
            Wettenhall,R.E., Kozma,S.C. and Thomas,G.
  TITLE     Dual requirement for a newly identified phosphorylation site in
            p70s6k
  JOURNAL   Mol Cell Biol 17 (9), 5648-5655 (1997)
   PUBMED   9271440
REFERENCE   9  (bases 1 to 5503)
  AUTHORS   Proud,C.G.
  TITLE     p70 S6 kinase: an enigma with variations
  JOURNAL   Trends Biochem Sci 21 (5), 181-185 (1996)
   PUBMED   8871403
  REMARK    Review article
REFERENCE   10 (bases 1 to 5503)
  AUTHORS   Grove,J.R., Banerjee,P., Balasubramanyam,A., Coffer,P.J.,
            Price,D.J., Avruch,J. and Woodgett,J.R.
  TITLE     Cloning and expression of two human p70 S6 kinase polypeptides
            differing only at their amino termini
  JOURNAL   Mol Cell Biol 11 (11), 5541-5550 (1991)
   PUBMED   1922062
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC004686.2.
            
            On Apr 15, 2019 this sequence version replaced XM_017024930.2.
            
            Summary: This gene encodes a member of the ribosomal S6 kinase
            family of serine/threonine kinases. The encoded protein responds to
            mTOR (mammalian target of rapamycin) signaling to promote protein
            synthesis, cell growth, and cell proliferation. Activity of this
            gene has been associated with human cancer. Alternatively spliced
            transcript variants have been observed. The use of alternative
            translation start sites results in isoforms with longer or shorter
            N-termini which may differ in their subcellular localizations.
            There are two pseudogenes for this gene on chromosome 17. [provided
            by RefSeq, Jan 2013].
            
            Transcript Variant: This variant (14), as well as variants 12, 13,
            and 15, encodes isoform l.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803612.51623.1,
                                           SRR1803613.25815.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            NMD candidate :: PMID: 23273915
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-205               AC004686.2         61945-62149         c
            206-255             AC004686.2         44659-44708         c
            256-376             AC004686.2         42466-42586         c
            377-445             AC004686.2         40567-40635         c
            446-593             AC004686.2         28688-28835         c
            594-651             AC004686.2         25096-25153         c
            652-726             AC004686.2         24838-24912         c
            727-827             AC004686.2         23548-23648         c
            828-918             AC004686.2         21010-21100         c
            919-1009            AC004686.2         20746-20836         c
            1010-1117           AC004686.2         19970-20077         c
            1118-1180           AC004686.2         18993-19055         c
            1181-1258           AC004686.2         18729-18806         c
            1259-1366           AC004686.2         14327-14434         c
            1367-1479           AC004686.2         9752-9864           c
            1480-5503           AC004686.2         4696-8719           c
FEATURES             Location/Qualifiers
     source          1..5503
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q23.1"
     gene            1..5503
                     /gene="RPS6KB1"
                     /gene_synonym="p70 S6KA; p70(S6K)-alpha; p70-alpha;
                     p70-S6K; PS6K; S6K; S6K-beta-1; S6K1; STK14A"
                     /note="ribosomal protein S6 kinase B1"
                     /db_xref="GeneID:6198"
                     /db_xref="HGNC:HGNC:10436"
                     /db_xref="MIM:608938"
     exon            1..205
                     /gene="RPS6KB1"
                     /gene_synonym="p70 S6KA; p70(S6K)-alpha; p70-alpha;
                     p70-S6K; PS6K; S6K; S6K-beta-1; S6K1; STK14A"
                     /inference="alignment:Splign:2.1.0"
     CDS             65..688
                     /gene="RPS6KB1"
                     /gene_synonym="p70 S6KA; p70(S6K)-alpha; p70-alpha;
                     p70-S6K; PS6K; S6K; S6K-beta-1; S6K1; STK14A"
                     /EC_number="2.7.11.1"
                     /note="isoform l is encoded by transcript variant 14;
                     serine/threonine kinase 14 alpha; ribosomal protein S6
                     kinase beta-1; ribosomal protein S6 kinase I;
                     serine/threonine-protein kinase 14A; ribosomal protein S6
                     kinase, 70kDa, polypeptide 1"
                     /codon_start=1
                     /product="ribosomal protein S6 kinase beta-1 isoform l"
                     /protein_id="NP_001356607.1"
                     /db_xref="GeneID:6198"
                     /db_xref="HGNC:HGNC:10436"
                     /db_xref="MIM:608938"
                     /translation="MRRRRRRDGFYPAPDFRDREAEDMAGVFDIDLDQPEDAGSEDEL
                     EEGGQLNESMDHGGVGPYELGMEHCEKFEISETSVNRGPEKIRPECFELLRVLGKGGY
                     GKVFQVRKVTGANTGKIFAMKVLKKAMIVRNAKDTAHTKAERNILEEVKHPFIVDLIY
                     AFQTGGKLYLILEYLSGGELFMQLEREGIFMEDTAWLEWNALHTSCS"
     exon            206..255
                     /gene="RPS6KB1"
                     /gene_synonym="p70 S6KA; p70(S6K)-alpha; p70-alpha;
                     p70-S6K; PS6K; S6K; S6K-beta-1; S6K1; STK14A"
                     /inference="alignment:Splign:2.1.0"
     exon            256..376
                     /gene="RPS6KB1"
                     /gene_synonym="p70 S6KA; p70(S6K)-alpha; p70-alpha;
                     p70-S6K; PS6K; S6K; S6K-beta-1; S6K1; STK14A"
                     /inference="alignment:Splign:2.1.0"
     exon            377..445
                     /gene="RPS6KB1"
                     /gene_synonym="p70 S6KA; p70(S6K)-alpha; p70-alpha;
                     p70-S6K; PS6K; S6K; S6K-beta-1; S6K1; STK14A"
                     /inference="alignment:Splign:2.1.0"
     exon            446..593
                     /gene="RPS6KB1"
                     /gene_synonym="p70 S6KA; p70(S6K)-alpha; p70-alpha;
                     p70-S6K; PS6K; S6K; S6K-beta-1; S6K1; STK14A"
                     /inference="alignment:Splign:2.1.0"
     exon            594..651
                     /gene="RPS6KB1"
                     /gene_synonym="p70 S6KA; p70(S6K)-alpha; p70-alpha;
                     p70-S6K; PS6K; S6K; S6K-beta-1; S6K1; STK14A"
                     /inference="alignment:Splign:2.1.0"
     exon            652..726
                     /gene="RPS6KB1"
                     /gene_synonym="p70 S6KA; p70(S6K)-alpha; p70-alpha;
                     p70-S6K; PS6K; S6K; S6K-beta-1; S6K1; STK14A"
                     /inference="alignment:Splign:2.1.0"
     exon            727..827
                     /gene="RPS6KB1"
                     /gene_synonym="p70 S6KA; p70(S6K)-alpha; p70-alpha;
                     p70-S6K; PS6K; S6K; S6K-beta-1; S6K1; STK14A"
                     /inference="alignment:Splign:2.1.0"
     exon            828..918
                     /gene="RPS6KB1"
                     /gene_synonym="p70 S6KA; p70(S6K)-alpha; p70-alpha;
                     p70-S6K; PS6K; S6K; S6K-beta-1; S6K1; STK14A"
                     /inference="alignment:Splign:2.1.0"
     exon            919..1009
                     /gene="RPS6KB1"
                     /gene_synonym="p70 S6KA; p70(S6K)-alpha; p70-alpha;
                     p70-S6K; PS6K; S6K; S6K-beta-1; S6K1; STK14A"
                     /inference="alignment:Splign:2.1.0"
     exon            1010..1117
                     /gene="RPS6KB1"
                     /gene_synonym="p70 S6KA; p70(S6K)-alpha; p70-alpha;
                     p70-S6K; PS6K; S6K; S6K-beta-1; S6K1; STK14A"
                     /inference="alignment:Splign:2.1.0"
     exon            1118..1180
                     /gene="RPS6KB1"
                     /gene_synonym="p70 S6KA; p70(S6K)-alpha; p70-alpha;
                     p70-S6K; PS6K; S6K; S6K-beta-1; S6K1; STK14A"
                     /inference="alignment:Splign:2.1.0"
     exon            1181..1258
                     /gene="RPS6KB1"
                     /gene_synonym="p70 S6KA; p70(S6K)-alpha; p70-alpha;
                     p70-S6K; PS6K; S6K; S6K-beta-1; S6K1; STK14A"
                     /inference="alignment:Splign:2.1.0"
     exon            1259..1366
                     /gene="RPS6KB1"
                     /gene_synonym="p70 S6KA; p70(S6K)-alpha; p70-alpha;
                     p70-S6K; PS6K; S6K; S6K-beta-1; S6K1; STK14A"
                     /inference="alignment:Splign:2.1.0"
     exon            1367..1479
                     /gene="RPS6KB1"
                     /gene_synonym="p70 S6KA; p70(S6K)-alpha; p70-alpha;
                     p70-S6K; PS6K; S6K; S6K-beta-1; S6K1; STK14A"
                     /inference="alignment:Splign:2.1.0"
     exon            1480..5503
                     /gene="RPS6KB1"
                     /gene_synonym="p70 S6KA; p70(S6K)-alpha; p70-alpha;
                     p70-S6K; PS6K; S6K; S6K-beta-1; S6K1; STK14A"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1826..1831
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RPS6KB1"
                     /gene_synonym="p70 S6KA; p70(S6K)-alpha; p70-alpha;
                     p70-S6K; PS6K; S6K; S6K-beta-1; S6K1; STK14A"
                     /note="hexamer: ATTACA"
     polyA_site      1856
                     /gene="RPS6KB1"
                     /gene_synonym="p70 S6KA; p70(S6K)-alpha; p70-alpha;
                     p70-S6K; PS6K; S6K; S6K-beta-1; S6K1; STK14A"
                     /note="major polyA site"
     regulatory      5328..5333
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RPS6KB1"
                     /gene_synonym="p70 S6KA; p70(S6K)-alpha; p70-alpha;
                     p70-S6K; PS6K; S6K; S6K-beta-1; S6K1; STK14A"
                     /note="hexamer: ATTAAA"
     polyA_site      5349
                     /gene="RPS6KB1"
                     /gene_synonym="p70 S6KA; p70(S6K)-alpha; p70-alpha;
                     p70-S6K; PS6K; S6K; S6K-beta-1; S6K1; STK14A"
                     /note="major polyA site"
     regulatory      5472..5477
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RPS6KB1"
                     /gene_synonym="p70 S6KA; p70(S6K)-alpha; p70-alpha;
                     p70-S6K; PS6K; S6K; S6K-beta-1; S6K1; STK14A"
                     /note="hexamer: AATAAA"
     polyA_site      5503
                     /gene="RPS6KB1"
                     /gene_synonym="p70 S6KA; p70(S6K)-alpha; p70-alpha;
                     p70-S6K; PS6K; S6K; S6K-beta-1; S6K1; STK14A"
ORIGIN      
        1 gcactgagcc taagcagccg gtgatggcgg cagcggctgt ggtggctgcg gcgggtccgg
       61 gcccatgagg cgacgaagga ggcgggacgg cttttaccca gccccggact tccgagacag
      121 ggaagctgag gacatggcag gagtgtttga catagacctg gaccagccag aggacgcggg
      181 ctctgaggat gagctggagg aggggggtca gttaaatgaa agcatggacc atgggggagt
      241 tggaccatat gaacttggca tggaacattg tgagaaattt gaaatctcag aaactagtgt
      301 gaacagaggg ccagaaaaaa tcagaccaga atgttttgag ctacttcggg tacttggtaa
      361 agggggctat ggaaaggttt ttcaagtacg aaaagtaaca ggagcaaata ctgggaaaat
      421 atttgccatg aaggtgctta aaaaggcaat gatagtaaga aatgctaaag atacagctca
      481 tacaaaagca gaacggaata ttctggagga agtaaagcat cccttcatcg tggatttaat
      541 ttatgccttt cagactggtg gaaaactcta cctcatcctt gagtatctca gtggaggaga
      601 actatttatg cagttagaaa gagagggaat atttatggaa gacactgcct ggcttgagtg
      661 gaacgctctt cacaccagtt gttcctaaca gaaccattct cattgcggct ttctttcctt
      721 gaattgcttt tacttggcag aaatctccat ggctttgggg catttacatc aaaaggggat
      781 catctacaga gacctgaagc cggagaatat catgcttaat caccaaggtc atgtgaaact
      841 aacagacttt ggactatgca aagaatctat tcatgatgga acagtcacac acacattttg
      901 tggaacaata gaatacatgg cccctgaaat cttgatgaga agtggccaca atcgtgctgt
      961 ggattggtgg agtttgggag cattaatgta tgacatgctg actggagcac ccccattcac
     1021 tggggagaat agaaagaaaa caattgacaa aatcctcaaa tgtaaactca atttgcctcc
     1081 ctacctcaca caagaagcca gagatctgct taaaaagctg ctgaaaagaa atgctgcttc
     1141 tcgtctggga gctggtcctg gggacgctgg agaagttcaa gctcatccat tctttagaca
     1201 cattaactgg gaagaacttc tggctcgaaa ggtggagccc ccctttaaac ctctgttgca
     1261 atctgaagag gatgtaagtc agtttgattc caagtttaca cgtcagacac ctgtcgacag
     1321 cccagatgac tcaactctca gtgaaagtgc caatcaggtc tttctgggtt ttacatatgt
     1381 ggctccatct gtacttgaaa gtgtgaaaga aaagttttcc tttgaaccaa aaatccgatc
     1441 acctcgaaga tttattggca gcccacgaac acctgtcagc ccagtcaaat tttctcctgg
     1501 ggatttctgg ggaagaggtg cttcggccag cacagcaaat cctcagacac ctgtggaata
     1561 cccaatggaa acaagtggca tagagcagat ggatgtgaca atgagtgggg aagcatcggc
     1621 accacttcca atacgacagc cgaactctgg gccatacaaa aaacaagctt ttcccatgat
     1681 ctccaaacgg ccagagcacc tgcgtatgaa tctatgacag agcaatgctt ttaatgaatt
     1741 taaggcaaaa aaggtggaga gggagatgtg tgagcatcct gcaaggtgaa acgactcaaa
     1801 atgacagttt cagagagtca atgtcattac atagaacact tcagacacag gaaaaataaa
     1861 cgtggatttt aaaaaatcaa tcaatggtgc aaaaaaaaac ttaaagcaaa atagtattgc
     1921 tgaactctta ggcacatcaa ttaattgatt cctcgcgaca tcttctcaac cttatcaagg
     1981 attttcatgt tgatgactcg aaactgacag tattaagggt aggatgttgc ttctgaatca
     2041 ctgttgagtt ctgattgtgt tgaagaaggg ttatcctttc attaggcaaa gtacaaaatt
     2101 gcctataata cttgcaacta aggacaaatt agcatgcaag cttggtcaaa ctttttccag
     2161 caaaatggaa gcaaagacaa aagaaactta ccaattgatg ttttacgtgc aaacaacctg
     2221 aatctttttt ttatataaat atatattttt caaatagatt tttgattcag ctcattatga
     2281 aaaacatccc aaactttaaa atgcgaaatt attggttggt gtgaagaaag ccagacaact
     2341 tctgtttctt ctcttggtga aataataaaa tgcaaatgaa tcattgttaa ccacagctgt
     2401 ggctcgtttg agggattggg gtggacctgg ggtttatttt cagtaaccca gctgcaatac
     2461 ctgtctgtaa tatgagaaaa aaaaaatgaa tctatttaat catttctact tgcagtactg
     2521 ctatgtgcta agcttaactg gaagccttgg aatgggcata agttgtatgt cctacatttc
     2581 atcattgtcc cgggcctgca ttgcactgga aaaaaaaatc gccacctgtt cttacaccag
     2641 tatttggttc aagacaccaa atgtcttcag cccatggctg aagaacaaca gaagagagtc
     2701 aggataaaaa atacatactg tggtcggcaa ggtgagggag atagggatat ccaggggaag
     2761 agggtgttgc tgtggcccac tctctgtcta atctctttac agcaaattgg taagattttc
     2821 agttttactt ctttctactg tttctgctgt ctaccttcct tatatttttt tcctcaacag
     2881 ttttaaaaag aaaaaaaggt ctattttttt ttctcctata cttgggctac attttttgat
     2941 tgtaaaaata tttgatggcc ttttgatgaa tgtcttccac agtaaagaaa acttagtggc
     3001 ttaatttagg aaacatgtta acaggacact atgtttttga aattgtaaca aaatctacat
     3061 aaatgattta caggttaaaa gaataaaaat aaaggtaact ttacctttct taaatatttc
     3121 ctgccttaaa gagagcattt ccatgacttt agctggtgaa agggtttaat atctgcagag
     3181 ctttataaaa atatatttca gtgcatactg gtataataga tgatcatgca gttgcagttg
     3241 agttgtatca ccttttttgt ttgtctttta taatgtcttc agtctgagtg tgcaaagtca
     3301 atttgtaata ttttgcaacc ctaggatttt tttaaataga tgctgcttgc tatgttttca
     3361 aacctttttg agccatagga tccaagccat aaaattcttt atgcatgttg aattcagtca
     3421 gaaaagagca aggctttgct ttttgaaatt gcaactcaaa tgagatggga tgaaatccta
     3481 tgacagtaag caaaaacaga accatgaaaa atgattggac atacaccttt tcaattgtgg
     3541 caataattga aagaatcgat aaaagttcat ctttggacag aaagccttta aaaaaaaaat
     3601 cactccctct tccccctcct cccttattgc agcagcctac tgagaacttt gactgttgct
     3661 ggtaaattag aagctacaat aataattaag ggcagaaatt atacttaaaa agtgcagatc
     3721 cttgttcttt gacaatttgt gatgtctgaa aaaacagaac ccgaaaagct atggtgatat
     3781 gtacaggcat tatttcagac tgtaaatggc ttgtgatact cttgatactt gttttcaaat
     3841 atgtttacta actgtagtgt tgactgcctg accaaattcc agtgaaactt atacaccaaa
     3901 atattcttcc taggtcctat ttgctagtaa catgagcact gtgattggct ggctataacc
     3961 accccagtta aaccattttc ataattagta gtgccagcaa tagtggcaaa cactgcaact
     4021 tttctgcata aaaagcatta attgcacagc taccatccac acaaatacat agtttttctg
     4081 acttcacatt tattaagtga aatttatttc ccatgctgtg gaaagtttat tgagaacttg
     4141 tttcataaat ggatatccct actatgactg tgaaaacatg tcaagtgtca cattagtgtc
     4201 acagacagaa agcacacacc tatgcaatat ggcttatcta tatttatttg taaaaatcca
     4261 agcatagttt aaaatatgat gtcgatatta ctagtcttga gtttctaaga gggttcttta
     4321 tgttatacca ggtaagtgta taaaagagat taagtgcttt tttttcatca cttgattatt
     4381 ttctttaaaa tcagctatta caggatattt ttttatttta tacatgctgt tttttaatta
     4441 aaatataatc actgaagttt actaatttga ttttataagg tttgtagcat tacagaataa
     4501 ctaaactggg atttataaac cagctgtgat taacaatgta aagtattaat tattgaactt
     4561 tgaaccagat ttttaggaaa attatgttct ttttccccct ttatggtctt aactaatttg
     4621 aatccttcaa gaaggatttt tccatactat tttttaagat agaagataat ttgtgggcag
     4681 gggtggagga tgcatgtatg atactccata aattcaacat tctttactat aggtaatgaa
     4741 tgattataaa caagatgcat cttagatagt attaatatac tgagccttgg attatatatt
     4801 taatatagga cctattttga atattcagtt aatcatatgg ttcctagctt acaagggcta
     4861 gatctaagat tattcccatg agaaatgttg aatttatgaa gaatagattt taaggctttg
     4921 aaaatggtta atttctcaaa aacatcaatg tccaaacatc tacctttttt cataggagta
     4981 gacactagca agctggacaa actatcacaa aagtatttgt cacacataac ctgtggtctg
     5041 ttgctgatta atacagtact ttttcttgtg tgattcttaa cattatagca caagtattat
     5101 ctcagtggat tatccggaat aacatctgaa agatgggttc atctatgttt gtgtttgctc
     5161 tttaaactat tgtttctcct atcccaagtt cgctttgcat ctatcagtaa ataaaattct
     5221 tcagctgcct tattaggagt gctatgaggg taacacctgt tctgcttttc atcttgtatt
     5281 tagttgactg tattatttga tttcggattg aatgaatgta aatagaaatt aaatgcaaat
     5341 ttgaatgaac ataaatagaa gtgatttatt tttttattat cttaaagata ggaaggaatt
     5401 agtatacgta tcaggtgtat aaatggttta aatttatttt aacatgtcag atgtgttcaa
     5461 gttgtcatat aaataaacta tgtatgtaag gaatgggtta aaa
//
