LOCUS       NM_004586               7987 bp    mRNA    linear   PRI 11-OCT-2024
DEFINITION  Homo sapiens ribosomal protein S6 kinase A3 (RPS6KA3), mRNA.
ACCESSION   NM_004586 XM_939339 XM_944112
VERSION     NM_004586.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7987)
  AUTHORS   Schaeffer,S., Gupta,B., Calatayud,A.L., Calderaro,J., Caruso,S.,
            Hirsch,T.Z., Pelletier,L., Zucman-Rossi,J. and Rebouissou,S.
  TITLE     RSK2 inactivation cooperates with AXIN1 inactivation or
            beta-catenin activation to promote hepatocarcinogenesis
  JOURNAL   J Hepatol 79 (3), 704-716 (2023)
   PUBMED   37201672
  REMARK    GeneRIF: RSK2 inactivation cooperates with AXIN1 inactivation or
            beta-catenin activation to promote hepatocarcinogenesis.
REFERENCE   2  (bases 1 to 7987)
  AUTHORS   Pan,J., Liu,M., Duan,X. and Wang,D.
  TITLE     A short peptide LINC00665_18aa encoded by lncRNA LINC00665
            suppresses the proliferation and migration of osteosarcoma cells
            through the regulation of the CREB1/RPS6KA3 interaction
  JOURNAL   PLoS One 18 (6), e0286422 (2023)
   PUBMED   37285335
  REMARK    GeneRIF: A short peptide LINC00665_18aa encoded by lncRNA LINC00665
            suppresses the proliferation and migration of osteosarcoma cells
            through the regulation of the CREB1/RPS6KA3 interaction.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 7987)
  AUTHORS   Lopez,J., Bonsor,D.A., Sale,M.J., Urisman,A., Mehalko,J.L.,
            Cabanski-Dunning,M., Castel,P., Simanshu,D.K. and McCormick,F.
  TITLE     The ribosomal S6 kinase 2 (RSK2)-SPRED2 complex regulates the
            phosphorylation of RSK substrates and MAPK signaling
  JOURNAL   J Biol Chem 299 (6), 104789 (2023)
   PUBMED   37149146
  REMARK    GeneRIF: The ribosomal S6 kinase 2 (RSK2)-SPRED2 complex regulates
            the phosphorylation of RSK substrates and MAPK signaling.
REFERENCE   4  (bases 1 to 7987)
  AUTHORS   Jin,H., Li,H. and Qiang,S.
  TITLE     Coffin-Lowry Syndrome Induced by RPS6KA3 Gene Variation in China: A
            Case Report in Twins
  JOURNAL   Medicina (Kaunas) 58 (7), 958 (2022)
   PUBMED   35888677
  REMARK    GeneRIF: Coffin-Lowry Syndrome Induced by RPS6KA3 Gene Variation in
            China: A Case Report in Twins.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 7987)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   6  (bases 1 to 7987)
  AUTHORS   Adams,M.D., Kerlavage,A.R., Fleischmann,R.D., Fuldner,R.A.,
            Bult,C.J., Lee,N.H., Kirkness,E.F., Weinstock,K.G., Gocayne,J.D.,
            White,O. et al.
  TITLE     Initial assessment of human gene diversity and expression patterns
            based upon 83 million nucleotides of cDNA sequence
  JOURNAL   Nature 377 (6547 Suppl), 3-174 (1995)
   PUBMED   7566098
REFERENCE   7  (bases 1 to 7987)
  AUTHORS   Zhao,Y., Bjorbaek,C., Weremowicz,S., Morton,C.C. and Moller,D.E.
  TITLE     RSK3 encodes a novel pp90rsk isoform with a unique N-terminal
            sequence: growth factor-stimulated kinase function and nuclear
            translocation
  JOURNAL   Mol Cell Biol 15 (8), 4353-4363 (1995)
   PUBMED   7623830
REFERENCE   8  (bases 1 to 7987)
  AUTHORS   Ku,N.O. and Omary,M.B.
  TITLE     Identification of the major physiologic phosphorylation site of
            human keratin 18: potential kinases and a role in filament
            reorganization
  JOURNAL   J Cell Biol 127 (1), 161-171 (1994)
   PUBMED   7523419
REFERENCE   9  (bases 1 to 7987)
  AUTHORS   Rogers,R.C. and Abidi,F.E.
  TITLE     RPS6KA3-Related Intellectual Disability
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301520
REFERENCE   10 (bases 1 to 7987)
  AUTHORS   Omary,M.B., Baxter,G.T., Chou,C.F., Riopel,C.L., Lin,W.Y. and
            Strulovici,B.
  TITLE     PKC epsilon-related kinase associates with and phosphorylates
            cytokeratin 8 and 18
  JOURNAL   J Cell Biol 117 (3), 583-593 (1992)
   PUBMED   1374067
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL807772.12, U08316.1,
            AB102662.1, BC015451.1, BU618685.1, W00439.1, AV684171.1,
            CK905714.1, CA843526.1, CA843287.1, AA317826.1, AL732366.7,
            BQ427696.1, AK022869.1 and AA858312.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 17, 2019 this sequence version replaced NM_004586.2.
            
            Summary: This gene encodes a member of the RSK (ribosomal S6
            kinase) family of serine/threonine kinases. This kinase contains 2
            non-identical kinase catalytic domains and phosphorylates various
            substrates, including members of the mitogen-activated kinase
            (MAPK) signalling pathway. The activity of this protein has been
            implicated in controlling cell growth and differentiation.
            Mutations in this gene have been associated with Coffin-Lowry
            syndrome (CLS). [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038196.2687120.1, AK313932.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000379565.9/ ENSP00000368884.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-277               AL807772.12        107650-107926       c
            278-1887            U08316.1           1-1610
            1888-1890           AB102662.1         1570-1572
            1891-2523           U08316.1           1614-2246
            2524-3075           BC015451.1         554-1105
            3076-3402           BU618685.1         251-577             c
            3403-3510           W00439.1           335-442
            3511-3977           AV684171.1         9-475
            3978-4267           CK905714.1         16-305              c
            4268-4275           CA843526.1         236-243
            4276-4546           CA843287.1         315-585             c
            4547-4644           AA317826.1         232-329
            4645-4985           AL732366.7         179205-179545       c
            4986-5284           BQ427696.1         2-300
            5285-7981           AK022869.1         1-2697
            7982-7987           AA858312.1         15-20               c
FEATURES             Location/Qualifiers
     source          1..7987
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xp22.12"
     gene            1..7987
                     /gene="RPS6KA3"
                     /gene_synonym="CLS; HU-3; ISPK-1; MAPKAPK1B; MRX19;
                     p90-RSK2; pp90RSK2; RSK; RSK2; S6K-alpha3; XLID19"
                     /note="ribosomal protein S6 kinase A3"
                     /db_xref="GeneID:6197"
                     /db_xref="HGNC:HGNC:10432"
                     /db_xref="MIM:300075"
     exon            1..346
                     /gene="RPS6KA3"
                     /gene_synonym="CLS; HU-3; ISPK-1; MAPKAPK1B; MRX19;
                     p90-RSK2; pp90RSK2; RSK; RSK2; S6K-alpha3; XLID19"
                     /inference="alignment:Splign:2.1.0"
     CDS             278..2500
                     /gene="RPS6KA3"
                     /gene_synonym="CLS; HU-3; ISPK-1; MAPKAPK1B; MRX19;
                     p90-RSK2; pp90RSK2; RSK; RSK2; S6K-alpha3; XLID19"
                     /EC_number="2.7.11.1"
                     /note="insulin-stimulated protein kinase 1; RSK-2; p90-RSK
                     3; MAPKAPK-1b; S6K-alpha-3; MAPKAP kinase 1b; ribosomal S6
                     kinase 2; MAPK-activated protein kinase 1b; MAP
                     kinase-activated protein kinase 1b; ribosomal protein S6
                     kinase, 90kDa, polypeptide 3; epididymis secretory sperm
                     binding protein"
                     /codon_start=1
                     /product="ribosomal protein S6 kinase alpha-3"
                     /protein_id="NP_004577.1"
                     /db_xref="CCDS:CCDS14197.1"
                     /db_xref="GeneID:6197"
                     /db_xref="HGNC:HGNC:10432"
                     /db_xref="MIM:300075"
                     /translation="MPLAQLADPWQKMAVESPSDSAENGQQIMDEPMGEEEINPQTEE
                     VSIKEIAITHHVKEGHEKADPSQFELLKVLGQGSFGKVFLVKKISGSDARQLYAMKVL
                     KKATLKVRDRVRTKMERDILVEVNHPFIVKLHYAFQTEGKLYLILDFLRGGDLFTRLS
                     KEVMFTEEDVKFYLAELALALDHLHSLGIIYRDLKPENILLDEEGHIKLTDFGLSKES
                     IDHEKKAYSFCGTVEYMAPEVVNRRGHTQSADWWSFGVLMFEMLTGTLPFQGKDRKET
                     MTMILKAKLGMPQFLSPEAQSLLRMLFKRNPANRLGAGPDGVEEIKRHSFFSTIDWNK
                     LYRREIHPPFKPATGRPEDTFYFDPEFTAKTPKDSPGIPPSANAHQLFRGFSFVAITS
                     DDESQAMQTVGVHSIVQQLHRNSIQFTDGYEVKEDIGVGSYSVCKRCIHKATNMEFAV
                     KIIDKSKRDPTEEIEILLRYGQHPNIITLKDVYDDGKYVYVVTELMKGGELLDKILRQ
                     KFFSEREASAVLFTITKTVEYLHAQGVVHRDLKPSNILYVDESGNPESIRICDFGFAK
                     QLRAENGLLMTPCYTANFVAPEVLKRQGYDAACDIWSLGVLLYTMLTGYTPFANGPDD
                     TPEEILARIGSGKFSLSGGYWNSVSDTAKDLVSKMLHVDPHQRLTAALVLRHPWIVHW
                     DQLPQYQLNRQDAPHLVKGAMAATYSALNRNQSPVLEPVGRSTLAQRRGIKKITSTAL
                     "
     exon            347..403
                     /gene="RPS6KA3"
                     /gene_synonym="CLS; HU-3; ISPK-1; MAPKAPK1B; MRX19;
                     p90-RSK2; pp90RSK2; RSK; RSK2; S6K-alpha3; XLID19"
                     /inference="alignment:Splign:2.1.0"
     exon            404..520
                     /gene="RPS6KA3"
                     /gene_synonym="CLS; HU-3; ISPK-1; MAPKAPK1B; MRX19;
                     p90-RSK2; pp90RSK2; RSK; RSK2; S6K-alpha3; XLID19"
                     /inference="alignment:Splign:2.1.0"
     exon            521..602
                     /gene="RPS6KA3"
                     /gene_synonym="CLS; HU-3; ISPK-1; MAPKAPK1B; MRX19;
                     p90-RSK2; pp90RSK2; RSK; RSK2; S6K-alpha3; XLID19"
                     /inference="alignment:Splign:2.1.0"
     exon            603..683
                     /gene="RPS6KA3"
                     /gene_synonym="CLS; HU-3; ISPK-1; MAPKAPK1B; MRX19;
                     p90-RSK2; pp90RSK2; RSK; RSK2; S6K-alpha3; XLID19"
                     /inference="alignment:Splign:2.1.0"
     exon            684..763
                     /gene="RPS6KA3"
                     /gene_synonym="CLS; HU-3; ISPK-1; MAPKAPK1B; MRX19;
                     p90-RSK2; pp90RSK2; RSK; RSK2; S6K-alpha3; XLID19"
                     /inference="alignment:Splign:2.1.0"
     exon            764..870
                     /gene="RPS6KA3"
                     /gene_synonym="CLS; HU-3; ISPK-1; MAPKAPK1B; MRX19;
                     p90-RSK2; pp90RSK2; RSK; RSK2; S6K-alpha3; XLID19"
                     /inference="alignment:Splign:2.1.0"
     exon            871..908
                     /gene="RPS6KA3"
                     /gene_synonym="CLS; HU-3; ISPK-1; MAPKAPK1B; MRX19;
                     p90-RSK2; pp90RSK2; RSK; RSK2; S6K-alpha3; XLID19"
                     /inference="alignment:Splign:2.1.0"
     exon            909..1051
                     /gene="RPS6KA3"
                     /gene_synonym="CLS; HU-3; ISPK-1; MAPKAPK1B; MRX19;
                     p90-RSK2; pp90RSK2; RSK; RSK2; S6K-alpha3; XLID19"
                     /inference="alignment:Splign:2.1.0"
     exon            1052..1122
                     /gene="RPS6KA3"
                     /gene_synonym="CLS; HU-3; ISPK-1; MAPKAPK1B; MRX19;
                     p90-RSK2; pp90RSK2; RSK; RSK2; S6K-alpha3; XLID19"
                     /inference="alignment:Splign:2.1.0"
     exon            1123..1211
                     /gene="RPS6KA3"
                     /gene_synonym="CLS; HU-3; ISPK-1; MAPKAPK1B; MRX19;
                     p90-RSK2; pp90RSK2; RSK; RSK2; S6K-alpha3; XLID19"
                     /inference="alignment:Splign:2.1.0"
     exon            1212..1276
                     /gene="RPS6KA3"
                     /gene_synonym="CLS; HU-3; ISPK-1; MAPKAPK1B; MRX19;
                     p90-RSK2; pp90RSK2; RSK; RSK2; S6K-alpha3; XLID19"
                     /inference="alignment:Splign:2.1.0"
     exon            1277..1379
                     /gene="RPS6KA3"
                     /gene_synonym="CLS; HU-3; ISPK-1; MAPKAPK1B; MRX19;
                     p90-RSK2; pp90RSK2; RSK; RSK2; S6K-alpha3; XLID19"
                     /inference="alignment:Splign:2.1.0"
     exon            1380..1504
                     /gene="RPS6KA3"
                     /gene_synonym="CLS; HU-3; ISPK-1; MAPKAPK1B; MRX19;
                     p90-RSK2; pp90RSK2; RSK; RSK2; S6K-alpha3; XLID19"
                     /inference="alignment:Splign:2.1.0"
     exon            1505..1630
                     /gene="RPS6KA3"
                     /gene_synonym="CLS; HU-3; ISPK-1; MAPKAPK1B; MRX19;
                     p90-RSK2; pp90RSK2; RSK; RSK2; S6K-alpha3; XLID19"
                     /inference="alignment:Splign:2.1.0"
     exon            1631..1720
                     /gene="RPS6KA3"
                     /gene_synonym="CLS; HU-3; ISPK-1; MAPKAPK1B; MRX19;
                     p90-RSK2; pp90RSK2; RSK; RSK2; S6K-alpha3; XLID19"
                     /inference="alignment:Splign:2.1.0"
     exon            1721..1879
                     /gene="RPS6KA3"
                     /gene_synonym="CLS; HU-3; ISPK-1; MAPKAPK1B; MRX19;
                     p90-RSK2; pp90RSK2; RSK; RSK2; S6K-alpha3; XLID19"
                     /inference="alignment:Splign:2.1.0"
     exon            1880..2041
                     /gene="RPS6KA3"
                     /gene_synonym="CLS; HU-3; ISPK-1; MAPKAPK1B; MRX19;
                     p90-RSK2; pp90RSK2; RSK; RSK2; S6K-alpha3; XLID19"
                     /inference="alignment:Splign:2.1.0"
     exon            2042..2118
                     /gene="RPS6KA3"
                     /gene_synonym="CLS; HU-3; ISPK-1; MAPKAPK1B; MRX19;
                     p90-RSK2; pp90RSK2; RSK; RSK2; S6K-alpha3; XLID19"
                     /inference="alignment:Splign:2.1.0"
     exon            2119..2236
                     /gene="RPS6KA3"
                     /gene_synonym="CLS; HU-3; ISPK-1; MAPKAPK1B; MRX19;
                     p90-RSK2; pp90RSK2; RSK; RSK2; S6K-alpha3; XLID19"
                     /inference="alignment:Splign:2.1.0"
     exon            2237..2377
                     /gene="RPS6KA3"
                     /gene_synonym="CLS; HU-3; ISPK-1; MAPKAPK1B; MRX19;
                     p90-RSK2; pp90RSK2; RSK; RSK2; S6K-alpha3; XLID19"
                     /inference="alignment:Splign:2.1.0"
     exon            2378..7987
                     /gene="RPS6KA3"
                     /gene_synonym="CLS; HU-3; ISPK-1; MAPKAPK1B; MRX19;
                     p90-RSK2; pp90RSK2; RSK; RSK2; S6K-alpha3; XLID19"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3055..3060
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RPS6KA3"
                     /gene_synonym="CLS; HU-3; ISPK-1; MAPKAPK1B; MRX19;
                     p90-RSK2; pp90RSK2; RSK; RSK2; S6K-alpha3; XLID19"
                     /note="hexamer: ATTAAA"
     polyA_site      3075
                     /gene="RPS6KA3"
                     /gene_synonym="CLS; HU-3; ISPK-1; MAPKAPK1B; MRX19;
                     p90-RSK2; pp90RSK2; RSK; RSK2; S6K-alpha3; XLID19"
                     /note="major polyA site"
     polyA_site      3173
                     /gene="RPS6KA3"
                     /gene_synonym="CLS; HU-3; ISPK-1; MAPKAPK1B; MRX19;
                     p90-RSK2; pp90RSK2; RSK; RSK2; S6K-alpha3; XLID19"
                     /note="major polyA site"
     regulatory      3562..3567
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RPS6KA3"
                     /gene_synonym="CLS; HU-3; ISPK-1; MAPKAPK1B; MRX19;
                     p90-RSK2; pp90RSK2; RSK; RSK2; S6K-alpha3; XLID19"
                     /note="hexamer: AATAAA"
     polyA_site      3589
                     /gene="RPS6KA3"
                     /gene_synonym="CLS; HU-3; ISPK-1; MAPKAPK1B; MRX19;
                     p90-RSK2; pp90RSK2; RSK; RSK2; S6K-alpha3; XLID19"
     regulatory      7966..7971
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RPS6KA3"
                     /gene_synonym="CLS; HU-3; ISPK-1; MAPKAPK1B; MRX19;
                     p90-RSK2; pp90RSK2; RSK; RSK2; S6K-alpha3; XLID19"
                     /note="hexamer: AATAAA"
     polyA_site      7987
                     /gene="RPS6KA3"
                     /gene_synonym="CLS; HU-3; ISPK-1; MAPKAPK1B; MRX19;
                     p90-RSK2; pp90RSK2; RSK; RSK2; S6K-alpha3; XLID19"
                     /note="major polyA site"
ORIGIN      
        1 agaaaggggc gagacccggt gcccagcgga ggcgcgaggc tctcgctcag cgcgcgggcg
       61 tctctggcca ggcgcgcggc tttcgggcgg tcggcgtcgg cgtcgagccc tctctggggc
      121 cgccgccgct gctgctgctg ctgccgctgc cgctgccgct gccgccgccg ccgccgccgc
      181 cgccgctgcc gtggggctcg ggcggcgacg gagcggggcc ggccgtgggg cgggcgggga
      241 gggaggaggc ggtgaaggcg gcgggccggg ggggaagatg ccgctggcgc agctggcgga
      301 cccgtggcag aagatggctg tggagagccc gtccgacagc gctgagaatg gacagcaaat
      361 tatggatgaa cctatgggag aggaggagat taacccacaa actgaagaag tcagtatcaa
      421 agaaattgca atcacacatc atgtaaagga aggacatgaa aaggcagatc cttcccagtt
      481 tgaactttta aaagtattag ggcagggatc atttggaaag gttttcttag ttaaaaaaat
      541 ctcaggctct gatgctaggc agctttatgc catgaaggta ttgaagaagg ccacactgaa
      601 agttcgagac cgagttcgga caaaaatgga acgtgatatc ttggtagagg ttaatcatcc
      661 ttttattgtc aagttgcatt atgcttttca aactgaaggg aagttgtatc ttattttgga
      721 ttttctcagg ggaggagatt tgtttacacg cttatccaaa gaggtgatgt tcacagaaga
      781 agatgtcaaa ttctacttgg ctgaacttgc acttgcttta gaccatctac atagcctggg
      841 aataatttat agagacttaa aaccagaaaa tatacttctt gatgaagaag gtcacatcaa
      901 gttaacagat ttcggcctaa gtaaagagtc tattgaccat gaaaagaagg catattcttt
      961 ttgtggaact gtggagtata tggctccaga agtagttaat cgtcgaggtc atactcagag
     1021 tgctgactgg tggtcttttg gtgtgttaat gtttgaaatg cttactggta cactcccttt
     1081 ccaaggaaaa gatcgaaaag aaacaatgac tatgattctt aaagccaaac ttggaatgcc
     1141 acagtttttg agtcctgaag cgcagagtct tttacgaatg cttttcaagc gaaatcctgc
     1201 aaacagatta ggtgcaggac cagatggagt tgaagaaatt aaaagacatt catttttctc
     1261 aacgatagac tggaataaac tgtatagaag agaaattcat ccgccattta aacctgcaac
     1321 gggcaggcct gaagatacat tctattttga tcctgagttt actgcaaaaa ctcccaaaga
     1381 ttcacctggc attccaccta gtgctaatgc acatcagctt tttcgggggt ttagttttgt
     1441 tgctattacc tcagatgatg aaagccaagc tatgcagaca gttggtgtac attcaattgt
     1501 tcagcagtta cacaggaaca gtattcagtt tactgatgga tatgaagtaa aagaagatat
     1561 tggagttggc tcctactctg tttgcaagag atgtatacat aaagctacaa acatggagtt
     1621 tgcagtgaag attattgata aaagcaagag agacccaaca gaagaaattg aaattcttct
     1681 tcgttatgga cagcatccaa acattatcac tctaaaggat gtatatgatg atggaaagta
     1741 tgtgtatgta gtaacagaac ttatgaaagg aggtgaattg ctggataaaa ttcttagaca
     1801 aaaatttttc tctgaacgag aggccagtgc tgtcctgttc actataacta aaaccgttga
     1861 atatcttcac gcacaagggg tggttcatag agacttgaaa cctagcaaca ttctttatgt
     1921 ggatgaatct ggtaatccgg aatctattcg aatttgtgat tttggctttg caaaacagct
     1981 gagagcggaa aatggtcttc tcatgactcc ttgttacact gcaaattttg ttgcaccaga
     2041 ggttttaaaa agacaaggct atgatgctgc ttgtgatata tggagtcttg gtgtcctact
     2101 ctatacaatg cttaccggtt acactccatt tgcaaatggt cctgatgata caccagagga
     2161 aatattggca cgaataggta gcggaaaatt ctcactcagt ggtggttact ggaattctgt
     2221 ttcagacaca gcaaaggacc tggtgtcaaa gatgcttcat gtagaccctc atcagagact
     2281 gactgctgct cttgtgctca gacatccttg gatcgtccac tgggaccaac tgccacaata
     2341 ccaactaaac agacaggatg caccacatct agtaaagggt gccatggcag ctacatattc
     2401 tgctttgaac cgtaatcagt caccagtttt ggaaccagta ggccgctcta ctcttgctca
     2461 gcggagaggt attaaaaaaa tcacctcaac agccctgtga agtgacctca gtgagatatt
     2521 tggtaccatg gtgtaagctg atagcacaag ttctggcgac aggtagcacg tatctgagag
     2581 acacctgcaa gcacacactg tcccagctgg tacccataat gctgctgttc ctgctgctgc
     2641 tcctgctttc ctctcttctc gctttaaatg attgttagca agttagattt tcctggagct
     2701 tcggaagaaa tgaaaatgga aacatgatga agatatagtc actgaatcaa tgagaaaaat
     2761 aatgaacata tgaataccac ctaaaaatac actgaataaa gtacaccata tagcattatt
     2821 tttataggaa atatttcatg tcccttaaat attctttgtt agttataggg atggacagtt
     2881 tatgttaagc acttagctta aacaatccgt ttatattagc actgtatccc ttgtgccatc
     2941 caacattttg tatgtttttg taaacagttc atatacagta catttctgta ctgctttctt
     3001 taatgtatac atgccttgtt taacttggaa tctattatta ttaatcaatt gactattaaa
     3061 tctggttaaa tagttcacct ggattaacag tattgttgga cagtcctaaa aatggccaga
     3121 ttgtggaaca gctgttgaat gtaatacttc caaaatgtac atatctttcc ccacgtctgt
     3181 ttcactggtt cgttcatttg tttgtttctt aaagtcaggt gctctgtcag actaacctag
     3241 agagctgtta tggtagagaa agttatcata tgtgtgtggc atgaaatcaa gaatacacct
     3301 atgaagttag tccatatact ttgcaactcc ttagagtact tttttcctta attaaggaag
     3361 tagtccttgc acttctaatc ttacatagca tccatactta gaatttggca tatcatctgg
     3421 gattttgcca atatacgtca aagcccttta agagtcatgg taaggagatg ggtgaaggaa
     3481 aatttagcaa caggtaattg aagtcctatt ggatatttca tgtttaaata gatattctat
     3541 attaaacact aatttaaatg taataaaggc caaaggcctc tgtatgaaat tggatttaaa
     3601 ctttcttatt ttagggaata aaacattatt gatcaaacag tatctgttct aacctaaaat
     3661 tataggtagg gcaggctaag tgaacagcat tgagtatttt ctgaatccct catgataatt
     3721 tatagccaca tactgcttcc tttgacttca ggaatgatca gttttcataa tggccactgg
     3781 gcctgcttag attgcagtat tcattatctg catctaattt ggtagtttcc acaatcgtat
     3841 ttgatgaaag aaacttcagt ccccattatt acctgtgtct ttgccaagct gcctagcata
     3901 catcagtatg taatgtaaaa gacatatgag caagaaaaaa gtgatttaac ttacctcatc
     3961 aagaatgtgc ccctacaggc cgggcgcagt ggctcacgcc tgtaatccca gcattttggg
     4021 aggccgaggc aggtggatca cctgaggtca ggagtttgag accagcctgg ccaacatggt
     4081 gaaaccccgt ctctactaaa aaatacaaaa attagctggg catggtggcg tgcacctgta
     4141 atcctagcta ctcaggaggc tgaggcagga gaatcgcttg aacctgggag gtggtggttg
     4201 cagtgagcca agatcacacc attgcactcc agcctgggtg acagagtgag actctgtctc
     4261 aagaaggaaa aaaaaaaaaa agaatgtact catacagatt tttgttagtc atcatgtaat
     4321 aagccattta tatcagaaac cttttttcct gccacagaag ctttaaattt tcccctaaga
     4381 gtacaaagcc aagagagtgt ggtttatcta aatgtcctga aatccacttg tcattcctta
     4441 atttctcctc tatgcaatta aagagaaagg ggaaatctat aacatttctt ttgcccaagt
     4501 gacacaaaaa tgattactgc atagaaatca aatgtaagtt tagcttctaa atgaaataat
     4561 atataggagg gatatcagaa tgtctagata gagaaagtat tttcaaattc ttgaatatat
     4621 ggatgctatt aaaagctgct ttccattgcc tggaaagaga gcttttttcc ctcctctatt
     4681 cagtgtttca aactttggtc atctcaaatt attgtcactt ttaaaatgtt aatgtcattc
     4741 tgagcagaaa tagatatatt ttcagatttt tttacactga tgctactccg taattgctta
     4801 ggagtattgt ccatcatagt attttagaac tagaagtaaa cacataaatc cttataaatt
     4861 acccaatcca actttctcat attgcatgta gagaacctga gacacatatc aaagtctaga
     4921 atccttaact tctccccatt ttgttttttt ccgcctcatc acaccatgct gcctttccct
     4981 catatgatca ccaaccagta gctgagttca aaaactacat atttcttggg cagtcctcta
     5041 ctggtatgtg atgccaactt ttatttcttt cgaagataat tgccttcttt gcctagccaa
     5101 gcagccaatt ttattggaga aaagcaagtt atatgttcac aaaatggaag gctttctacc
     5161 taaatttatt tcatctgtgg tcctcttatt ctcttccatt gcttttgaca tacagtggaa
     5221 aaacttggcc aacgatgtgc tctaaatata gtgcttgtgt tgggttaact tttccattaa
     5281 tttctcaaca tcaattattc aggcccctac agttgtggta ttgaggcccc taaaggcttc
     5341 tgcctttcac caattagtgc tgccagttac tgaggcttct cccaaaaggt atcagtccag
     5401 tcttaaagat atttcattgt gaaagaagaa actaaactat caggcctctt ttacaaaatg
     5461 agagattgaa tttaagcttg tcaagcacgt actggaaggt atgaattaca ctaccatgtg
     5521 ttttgtatct tccctttcaa gtgatgatgt taaatgaagg taagtttttc atcctttttt
     5581 aatttttgtt ttttataaat catttcagct ttttctggtt tatagaggtg tcttatttct
     5641 aatgcaacag acccccaact ttaacagatt tgatatggat gcatttattc acaagcaacc
     5701 ccaaaagtcc aaaaatgtaa taattttgac aaggcccaaa gttgagatgc tatgaagctt
     5761 gtgtgtgtga aaagtcagtt atgattgtct ggagagaagc tgtggtgtgt atgctgtaga
     5821 gtttccacat ttcacatgca gtgtactcca aaaagcgggt ttgggtcaac catttcccat
     5881 ctctttttaa gaagtgactg ctgttggggc caggggacat catgggaggt ggggctgtcc
     5941 agttagctgt gccctgcacc ttcagcccag gaaagatttg aacagagcaa aggcttcaag
     6001 agagggcaga atgtattcgg cagaaaaggg actcaggtaa gcccagcctg ggatgagagc
     6061 agaaaagcat tcaagatttg caggccttgc tatggatgcg cttaatcacc atggaggcca
     6121 gcaataccca tctcagcatg gctttgatat tctctacttc ctggccttta aaaatgacct
     6181 ataatttttc agtttgcttt actatatttt ataaagaaaa ttctatctta tggttgattg
     6241 agcattgaga cttatgaagg cattaggata gatagctcag gaatgtaaag gttcagaaaa
     6301 ggtctgtttt ctcagattaa caaatatgat ggattccatg gctgaccttg gtgcttaaac
     6361 caggaggttt caatctagtc ctagagttgt gtccctctga aaggcccaat gccatgtaac
     6421 taactttaaa ctggatatat actttgagcc ttacttaatt cacagataag ttgacttaac
     6481 tcagtatttt tatttcaatt aatgaaaaca gtcctctttt caaccccagg ttgcttacat
     6541 tttgctggtc tccccaagtg accattggtg gagaccaatt aatgaaggaa tgaaattcac
     6601 tttattggga ctgtggtatt caacagagcc acacttaacc actttttcca atgaagaatc
     6661 tccagaatga taatgcccaa atatggatgg ccaagaagaa tttgtatcta cggtgtgctt
     6721 tatgtgtttt tgacactgct gtattctgtg tgatcaagtg atttgcagct ggttccaatg
     6781 tgactgagtg ttctcaaaga tttctagtaa ctaagtcaac ttaattttct taagcctggt
     6841 attactatca gcctcacatt taccactttg attctagttt tttaactgtt cataacaggg
     6901 cataccgagg gttgggatga gagcctactt cctacctctt aaggcacttt cctcattatt
     6961 ttgccatata atcttgaact gcatgataag ctgtttaaat gtccatgact tctcccagag
     7021 caactagcaa agtatatgac attttgaata gagattagtg gaaaggaaaa tgtagagatt
     7081 taagttcaga ggtacaaacc tcaataaaac ctccttagag cacataccat gagtagagta
     7141 tatgtaaacg tatatgagaa gagagtcgct ataatagtcc tctctaagta gattagttca
     7201 ttgtctaatg gaagtgaaca tctttactcc tgttatcacc atagtactga atgaactgta
     7261 taatgagttt ttcaaacaag tatttaaaat agaactttca cgtacaaagc aagggcataa
     7321 agattttggt ttgtgtttct gtaagtgtag tttaccatct aaagctttgg tttttaattt
     7381 taaaaaaagc ttcaaggtat tgccattcct tagcatcctt attgattatg ggtgatgagt
     7441 ttttaactct taaaatcata tacgtgtatt agtttatctt aactgttgcc ctaagcaaaa
     7501 gggaaggtat acacctaagg gatgtactta ttttgcattt ttggtcatgg gtgggaatgg
     7561 ggtgggtgcc tacttaaata gtttttaaaa aagaaaaaaa tcacaaatat ttttctactg
     7621 ttatatatga tcttccttta tactagtttc tctctagtaa ggcatgccag aagcccaaga
     7681 taccatatca tgaattctta catattgtac cttttgttgg tggttaaatc gcatcagatg
     7741 cttggcattg ctgccataat tataaaatgc ttgtaaagga tcaaatatca ctaaatactt
     7801 taaattgttt tacttaagag tctaatctgg gaagttttca aatcatacta ttaatgtgta
     7861 atctaagctc ttcagatgta tccatgaata atcctggaac aatattgctt gtattcctgt
     7921 catagaacag gttttgtaat ctttaaaaga aatgaaaatt tatataataa agtttcaaat
     7981 caatgca
//
