LOCUS       NM_021135               5832 bp    mRNA    linear   PRI 15-MAR-2025
DEFINITION  Homo sapiens ribosomal protein S6 kinase A2 (RPS6KA2), transcript
            variant 1, mRNA.
ACCESSION   NM_021135
VERSION     NM_021135.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5832)
  AUTHORS   Huna,A., Flaman,J.M., Lodillinsky,C., Zhu,K., Makulyte,G.,
            Pakulska,V., Coute,Y., Ruisseaux,C., Saintigny,P.,
            Hernandez-Vargas,H., Defossez,P.A., Boissan,M., Martin,N. and
            Bernard,D.
  TITLE     RSK3 switches cell fate: from stress-induced senescence to
            malignant progression
  JOURNAL   J Exp Clin Cancer Res 42 (1), 318 (2023)
   PUBMED   38008756
  REMARK    GeneRIF: RSK3 switches cell fate: from stress-induced senescence to
            malignant progression.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5832)
  AUTHORS   Jiang,X., Du,W., Yang,C., Wang,S., Li,Y., Shen,X., Yang,X., Yao,J.,
            Du,R., Zhang,X., Huang,Y. and Shen,W.
  TITLE     TBX21 attenuates colorectal cancer progression via an
            ARHGAP29/RSK/GSK3beta dependent manner
  JOURNAL   Cell Oncol (Dordr) 46 (5), 1269-1283 (2023)
   PUBMED   37067748
  REMARK    GeneRIF: TBX21 attenuates colorectal cancer progression via an
            ARHGAP29/RSK/GSK3beta dependent manner.
REFERENCE   3  (bases 1 to 5832)
  AUTHORS   Segal,D., Maier,S., Mastromarco,G.J., Qian,W.W., Nabeel-Shah,S.,
            Lee,H., Moore,G., Lacoste,J., Larsen,B., Lin,Z.Y.,
            Selvabaskaran,A., Liu,K., Smibert,C., Zhang,Z., Greenblatt,J.,
            Peng,J., Lee,H.O., Gingras,A.C. and Taipale,M.
  TITLE     A central chaperone-like role for 14-3-3 proteins in human cells
  JOURNAL   Mol Cell 83 (6), 974-993 (2023)
   PUBMED   36931259
REFERENCE   4  (bases 1 to 5832)
  AUTHORS   Kumari,A., Gesumaria,L., Liu,Y.J., Hughitt,V.K., Zhang,X.,
            Ceribelli,M., Wilson,K.M., Klumpp-Thomas,C., Chen,L., McKnight,C.,
            Itkin,Z., Thomas,C.J., Mock,B.A., Schrump,D.S. and Chen,H.
  TITLE     mTOR inhibition overcomes RSK3-mediated resistance to BET
            inhibitors in small cell lung cancer
  JOURNAL   JCI Insight 8 (5), e156657 (2023)
   PUBMED   36883564
  REMARK    GeneRIF: mTOR inhibition overcomes RSK3-mediated resistance to BET
            inhibitors in small cell lung cancer.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5832)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   6  (bases 1 to 5832)
  AUTHORS   Zhao,Y., Bjorbaek,C. and Moller,D.E.
  TITLE     Regulation and interaction of pp90(rsk) isoforms with
            mitogen-activated protein kinases
  JOURNAL   J Biol Chem 271 (47), 29773-29779 (1996)
   PUBMED   8939914
REFERENCE   7  (bases 1 to 5832)
  AUTHORS   Xing,J., Ginty,D.D. and Greenberg,M.E.
  TITLE     Coupling of the RAS-MAPK pathway to gene activation by RSK2, a
            growth factor-regulated CREB kinase
  JOURNAL   Science 273 (5277), 959-963 (1996)
   PUBMED   8688081
REFERENCE   8  (bases 1 to 5832)
  AUTHORS   Wong,E.V., Schaefer,A.W., Landreth,G. and Lemmon,V.
  TITLE     Involvement of p90rsk in neurite outgrowth mediated by the cell
            adhesion molecule L1
  JOURNAL   J Biol Chem 271 (30), 18217-18223 (1996)
   PUBMED   8663493
REFERENCE   9  (bases 1 to 5832)
  AUTHORS   Zhao,Y., Bjorbaek,C., Weremowicz,S., Morton,C.C. and Moller,D.E.
  TITLE     RSK3 encodes a novel pp90rsk isoform with a unique N-terminal
            sequence: growth factor-stimulated kinase function and nuclear
            translocation
  JOURNAL   Mol Cell Biol 15 (8), 4353-4363 (1995)
   PUBMED   7623830
REFERENCE   10 (bases 1 to 5832)
  AUTHORS   Moller,D.E., Xia,C.H., Tang,W., Zhu,A.X. and Jakubowski,M.
  TITLE     Human rsk isoforms: cloning and characterization of tissue-specific
            expression
  JOURNAL   Am J Physiol 266 (2 Pt 1), C351-C359 (1994)
   PUBMED   8141249
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from HY150133.1, BC002363.2,
            AL022069.1, AB209116.1 and BQ029058.1.
            
            On Nov 23, 2018 this sequence version replaced NM_021135.5.
            
            Summary: This gene encodes a member of the RSK (ribosomal S6
            kinase) family of serine/threonine kinases. This kinase contains
            two non-identical kinase catalytic domains and phosphorylates
            various substrates, including members of the mitogen-activated
            kinase (MAPK) signalling pathway. The activity of this protein has
            been implicated in controlling cell growth and differentiation.
            Alternative splice variants, encoding different isoforms, have been
            characterized. [provided by RefSeq, Jan 2016].
            
            Transcript Variant: This variant (1) encodes isoform a.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC002363.2, SRR11853564.24097.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000265678.9/ ENSP00000265678.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-340               HY150133.1         28-367
            341-2379            BC002363.2         327-2365
            2380-2663           AL022069.1         104087-104370
            2664-4091           AB209116.1         2651-4078
            4092-4092           AL022069.1         105799-105799
            4093-5414           AB209116.1         4079-5400
            5415-5832           BQ029058.1         14-431              c
FEATURES             Location/Qualifiers
     source          1..5832
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q27"
     gene            1..5832
                     /gene="RPS6KA2"
                     /gene_synonym="HU-2; MAPKAPK1C; p90-RSK3; p90RSK2;
                     pp90RSK3; RSK; RSK3; S6K-alpha; S6K-alpha2"
                     /note="ribosomal protein S6 kinase A2"
                     /db_xref="GeneID:6196"
                     /db_xref="HGNC:HGNC:10431"
                     /db_xref="MIM:601685"
     exon            1..331
                     /gene="RPS6KA2"
                     /gene_synonym="HU-2; MAPKAPK1C; p90-RSK3; p90RSK2;
                     pp90RSK3; RSK; RSK3; S6K-alpha; S6K-alpha2"
                     /inference="alignment:Splign:2.1.0"
     CDS             233..2434
                     /gene="RPS6KA2"
                     /gene_synonym="HU-2; MAPKAPK1C; p90-RSK3; p90RSK2;
                     pp90RSK3; RSK; RSK3; S6K-alpha; S6K-alpha2"
                     /EC_number="2.7.11.1"
                     /note="isoform a is encoded by transcript variant 1;
                     ribosomal S6 kinase 3; ribosomal protein S6 kinase
                     alpha-2; MAPK-activated protein kinase 1c; ribosomal
                     protein S6 kinase, 90kDa, polypeptide 2; MAP
                     kinase-activated protein kinase 1c; mitogen-activated
                     protein kinase-activated protein kinase 1C"
                     /codon_start=1
                     /product="ribosomal protein S6 kinase alpha-2 isoform a"
                     /protein_id="NP_066958.2"
                     /db_xref="CCDS:CCDS5294.1"
                     /db_xref="GeneID:6196"
                     /db_xref="HGNC:HGNC:10431"
                     /db_xref="MIM:601685"
                     /translation="MDLSMKKFAVRRFFSVYLRRKSRSKSSSLSRLEEEGVVKEIDIS
                     HHVKEGFEKADPSQFELLKVLGQGSYGKVFLVRKVKGSDAGQLYAMKVLKKATLKVRD
                     RVRSKMERDILAEVNHPFIVKLHYAFQTEGKLYLILDFLRGGDLFTRLSKEVMFTEED
                     VKFYLAELALALDHLHSLGIIYRDLKPENILLDEEGHIKITDFGLSKEAIDHDKRAYS
                     FCGTIEYMAPEVVNRRGHTQSADWWSFGVLMFEMLTGSLPFQGKDRKETMALILKAKL
                     GMPQFLSGEAQSLLRALFKRNPCNRLGAGIDGVEEIKRHPFFVTIDWNTLYRKEIKPP
                     FKPAVGRPEDTFHFDPEFTARTPTDSPGVPPSANAHHLFRGFSFVASSLIQEPSQQDL
                     HKVPVHPIVQQLHGNNIHFTDGYEIKEDIGVGSYSVCKRCVHKATDTEYAVKIIDKSK
                     RDPSEEIEILLRYGQHPNIITLKDVYDDGKFVYLVMELMRGGELLDRILRQRYFSERE
                     ASDVLCTITKTMDYLHSQGVVHRDLKPSNILYRDESGSPESIRVCDFGFAKQLRAGNG
                     LLMTPCYTANFVAPEVLKRQGYDAACDIWSLGILLYTMLAGFTPFANGPDDTPEEILA
                     RIGSGKYALSGGNWDSISDAAKDVVSKMLHVDPHQRLTAMQVLKHPWVVNREYLSPNQ
                     LSRQDVHLVKGAMAATYFALNRTPQAPRLEPVLSSNLAQRRGMKRLTSTRL"
     exon            332..448
                     /gene="RPS6KA2"
                     /gene_synonym="HU-2; MAPKAPK1C; p90-RSK3; p90RSK2;
                     pp90RSK3; RSK; RSK3; S6K-alpha; S6K-alpha2"
                     /inference="alignment:Splign:2.1.0"
     exon            449..530
                     /gene="RPS6KA2"
                     /gene_synonym="HU-2; MAPKAPK1C; p90-RSK3; p90RSK2;
                     pp90RSK3; RSK; RSK3; S6K-alpha; S6K-alpha2"
                     /inference="alignment:Splign:2.1.0"
     exon            531..611
                     /gene="RPS6KA2"
                     /gene_synonym="HU-2; MAPKAPK1C; p90-RSK3; p90RSK2;
                     pp90RSK3; RSK; RSK3; S6K-alpha; S6K-alpha2"
                     /inference="alignment:Splign:2.1.0"
     exon            612..691
                     /gene="RPS6KA2"
                     /gene_synonym="HU-2; MAPKAPK1C; p90-RSK3; p90RSK2;
                     pp90RSK3; RSK; RSK3; S6K-alpha; S6K-alpha2"
                     /inference="alignment:Splign:2.1.0"
     exon            692..798
                     /gene="RPS6KA2"
                     /gene_synonym="HU-2; MAPKAPK1C; p90-RSK3; p90RSK2;
                     pp90RSK3; RSK; RSK3; S6K-alpha; S6K-alpha2"
                     /inference="alignment:Splign:2.1.0"
     exon            799..836
                     /gene="RPS6KA2"
                     /gene_synonym="HU-2; MAPKAPK1C; p90-RSK3; p90RSK2;
                     pp90RSK3; RSK; RSK3; S6K-alpha; S6K-alpha2"
                     /inference="alignment:Splign:2.1.0"
     exon            837..979
                     /gene="RPS6KA2"
                     /gene_synonym="HU-2; MAPKAPK1C; p90-RSK3; p90RSK2;
                     pp90RSK3; RSK; RSK3; S6K-alpha; S6K-alpha2"
                     /inference="alignment:Splign:2.1.0"
     exon            980..1050
                     /gene="RPS6KA2"
                     /gene_synonym="HU-2; MAPKAPK1C; p90-RSK3; p90RSK2;
                     pp90RSK3; RSK; RSK3; S6K-alpha; S6K-alpha2"
                     /inference="alignment:Splign:2.1.0"
     exon            1051..1139
                     /gene="RPS6KA2"
                     /gene_synonym="HU-2; MAPKAPK1C; p90-RSK3; p90RSK2;
                     pp90RSK3; RSK; RSK3; S6K-alpha; S6K-alpha2"
                     /inference="alignment:Splign:2.1.0"
     exon            1140..1204
                     /gene="RPS6KA2"
                     /gene_synonym="HU-2; MAPKAPK1C; p90-RSK3; p90RSK2;
                     pp90RSK3; RSK; RSK3; S6K-alpha; S6K-alpha2"
                     /inference="alignment:Splign:2.1.0"
     exon            1205..1307
                     /gene="RPS6KA2"
                     /gene_synonym="HU-2; MAPKAPK1C; p90-RSK3; p90RSK2;
                     pp90RSK3; RSK; RSK3; S6K-alpha; S6K-alpha2"
                     /inference="alignment:Splign:2.1.0"
     exon            1308..1438
                     /gene="RPS6KA2"
                     /gene_synonym="HU-2; MAPKAPK1C; p90-RSK3; p90RSK2;
                     pp90RSK3; RSK; RSK3; S6K-alpha; S6K-alpha2"
                     /inference="alignment:Splign:2.1.0"
     exon            1439..1564
                     /gene="RPS6KA2"
                     /gene_synonym="HU-2; MAPKAPK1C; p90-RSK3; p90RSK2;
                     pp90RSK3; RSK; RSK3; S6K-alpha; S6K-alpha2"
                     /inference="alignment:Splign:2.1.0"
     exon            1565..1654
                     /gene="RPS6KA2"
                     /gene_synonym="HU-2; MAPKAPK1C; p90-RSK3; p90RSK2;
                     pp90RSK3; RSK; RSK3; S6K-alpha; S6K-alpha2"
                     /inference="alignment:Splign:2.1.0"
     exon            1655..1813
                     /gene="RPS6KA2"
                     /gene_synonym="HU-2; MAPKAPK1C; p90-RSK3; p90RSK2;
                     pp90RSK3; RSK; RSK3; S6K-alpha; S6K-alpha2"
                     /inference="alignment:Splign:2.1.0"
     exon            1814..1975
                     /gene="RPS6KA2"
                     /gene_synonym="HU-2; MAPKAPK1C; p90-RSK3; p90RSK2;
                     pp90RSK3; RSK; RSK3; S6K-alpha; S6K-alpha2"
                     /inference="alignment:Splign:2.1.0"
     exon            1976..2052
                     /gene="RPS6KA2"
                     /gene_synonym="HU-2; MAPKAPK1C; p90-RSK3; p90RSK2;
                     pp90RSK3; RSK; RSK3; S6K-alpha; S6K-alpha2"
                     /inference="alignment:Splign:2.1.0"
     exon            2053..2170
                     /gene="RPS6KA2"
                     /gene_synonym="HU-2; MAPKAPK1C; p90-RSK3; p90RSK2;
                     pp90RSK3; RSK; RSK3; S6K-alpha; S6K-alpha2"
                     /inference="alignment:Splign:2.1.0"
     exon            2171..2308
                     /gene="RPS6KA2"
                     /gene_synonym="HU-2; MAPKAPK1C; p90-RSK3; p90RSK2;
                     pp90RSK3; RSK; RSK3; S6K-alpha; S6K-alpha2"
                     /inference="alignment:Splign:2.1.0"
     exon            2309..5832
                     /gene="RPS6KA2"
                     /gene_synonym="HU-2; MAPKAPK1C; p90-RSK3; p90RSK2;
                     pp90RSK3; RSK; RSK3; S6K-alpha; S6K-alpha2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5785..5790
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RPS6KA2"
                     /gene_synonym="HU-2; MAPKAPK1C; p90-RSK3; p90RSK2;
                     pp90RSK3; RSK; RSK3; S6K-alpha; S6K-alpha2"
                     /note="hexamer: AATAAA"
     polyA_site      5810
                     /gene="RPS6KA2"
                     /gene_synonym="HU-2; MAPKAPK1C; p90-RSK3; p90RSK2;
                     pp90RSK3; RSK; RSK3; S6K-alpha; S6K-alpha2"
                     /note="major polyA site"
     polyA_site      5832
                     /gene="RPS6KA2"
                     /gene_synonym="HU-2; MAPKAPK1C; p90-RSK3; p90RSK2;
                     pp90RSK3; RSK; RSK3; S6K-alpha; S6K-alpha2"
ORIGIN      
        1 actcgcaggg gaggcggaga aggaggcgga gggagcgatt gtggccccgg ccgcggtggc
       61 cggcgcggcc tgccctttgt gaccgcagct cgcgccccac gccccgcgcc catggccgcc
      121 gtgccgggct ccctggccac gcgtgcccgc ccgcggacct gagccccgcg cctgggatgc
      181 cggggatgcg cgtcccccgg ccctgcggct gctccgggct gggcgcgggg cgatggacct
      241 gagcatgaag aagttcgccg tgcgcaggtt cttctctgtg tacctgcgca ggaagtcgcg
      301 ctccaagagc tccagcctga gccggctcga ggaagaaggc gtcgtgaagg agatagacat
      361 cagccatcat gtgaaggagg gctttgagaa ggcagatcct tcccagtttg agctgctgaa
      421 ggttttagga caaggatcct atggaaaggt gttcctggtg aggaaggtga aggggtccga
      481 cgctgggcag ctctacgcca tgaaggtcct taagaaagcc accctaaaag ttcgggaccg
      541 agtgagatcg aagatggaga gagacatctt ggcagaagtg aatcacccct tcattgtgaa
      601 gcttcattat gcctttcaga cggaaggaaa gctctacctg atcctggact tcctgcgggg
      661 aggggacctc ttcacccggc tctccaaaga ggtcatgttc acggaggagg atgtcaagtt
      721 ctacctggct gagctggcct tggctttaga ccatctccac agcctgggga tcatctacag
      781 agatctgaag cctgagaaca tcctcctgga tgaagagggg cacattaaga tcacagattt
      841 cggcctgagt aaggaggcca ttgaccacga caagagagcg tactccttct gcgggacgat
      901 cgagtacatg gcgcccgagg tggtgaaccg gcgaggacac acgcagagtg ccgactggtg
      961 gtccttcggc gtgctcatgt ttgagatgct cacggggtcc ctgccgttcc aggggaagga
     1021 caggaaggag accatggctc tcatcctcaa agccaagctg gggatgccgc agttcctcag
     1081 tggggaggca cagagtttgc tgcgagctct cttcaaacgg aacccctgca accggctggg
     1141 tgctggcatt gacggagtgg aggaaattaa gcgccatccc ttctttgtga ccatagactg
     1201 gaacacgctg taccggaagg agatcaagcc accgttcaaa ccagcagtgg gcaggcctga
     1261 ggacaccttc cactttgacc ccgagttcac agcgcggacg cccacagact ctcctggcgt
     1321 ccccccgagt gcaaacgctc atcacctgtt tagaggattc agctttgtgg cctcaagcct
     1381 gatccaggag ccctcacagc aagatctgca caaagtccca gttcacccaa tcgtgcagca
     1441 gttacacggg aacaacatcc acttcaccga tggctacgag atcaaggagg acatcggggt
     1501 gggctcctac tcagtgtgca agcgatgtgt gcataaagcc acagacaccg agtatgccgt
     1561 gaagatcatt gataagagca agagagaccc ctcggaagag attgagatcc tcctgcggta
     1621 cggccagcac ccgaacatca tcaccctcaa ggatgtctat gatgatggca agtttgtgta
     1681 cctggtaatg gagctgatgc gtggtgggga gctcctggac cgcatcctcc ggcagagata
     1741 cttctcggag cgcgaagcca gtgacgtcct gtgcaccatc accaagacca tggactacct
     1801 ccattcccag ggggttgttc atcgagacct gaagccgagt aacatcctgt acagggatga
     1861 gtcggggagc ccagaatcca tccgagtctg cgacttcggc tttgccaagc agctgcgcgc
     1921 ggggaacggg ctgctcatga caccctgcta cacggccaat ttcgtggccc cggaggtcct
     1981 gaagcgtcaa ggctatgatg cggcgtgtga catctggagt ttggggatcc tgttgtacac
     2041 catgctggca ggatttaccc cttttgcaaa tgggccagac gatacccctg aggagattct
     2101 ggcgcggatc ggcagtggga agtatgccct ttctggggga aactgggact cgatatctga
     2161 cgcagctaaa gacgtcgtgt ccaagatgct ccacgtggac cctcatcagc gcctgacggc
     2221 gatgcaagtg ctcaaacacc cgtgggtggt caacagagag tacctgtccc caaaccagct
     2281 cagccgacag gacgtgcacc tggtgaaggg cgcgatggcc gccacctact ttgctctaaa
     2341 cagaacacct caggccccgc ggctggagcc cgtgctgtca tccaacctgg ctcagcgcag
     2401 aggcatgaag agactcacgt ccacgcggct gtagcgggtg ggaccctggc cccagcgtcc
     2461 cctgccagca tcctcgtggg ctcacagacc ccggcctcgg agcccgtctg gcacccagag
     2521 tgaccacaag tccagcaggg aggcggcgcc cgccctcgcc gtgtccgtgt tttctttttc
     2581 agccccggag agggtcctga cctgggggct tctccaagcc tcactgcgcc agcctccccg
     2641 cccgctctct tttctcccaa gcgaaaccaa atgcgcccct tcacctcgcg tgcccgtgcg
     2701 aggccggggg cttctttcag agcccgcggg tcctctcata catggcttct gtttctgccg
     2761 agagatctgt tttccaatta tgaagccggt cggtttggtc agactcccga cacccacgtc
     2821 ccaggtaccc ggtgggaaag tggcagtgcg agggcgcagc cattggtggt tgcagggccc
     2881 cagagggctg gggtgacctg gcatcccggg gctccccacg ggctggatga cggggttggc
     2941 actgtggcgt ccaggaggag atgcctggtt ctgcccaaaa taatccaaag agccgtttcc
     3001 tcctcgccct tcagtttttg cctgaggtgc tgggtagccc atcctttcct ctgtcccaga
     3061 ttcaaatgag gagtaagagc ccagacgaga ggaaggcagg ctggatcttt gccttgagag
     3121 ctccgtgtca ccaggatgga agggggtgcc tctcggagga gcctgtgtcc acctccagtc
     3181 tcggctttcc ccggggggcc aagcgcactg ggctgccgtc tgtccccagc tcccgtggcc
     3241 acacagctat ctggaggctt tgcagggagt cgtgggttct cgcacctgct cagccctgtg
     3301 tcggcttcct gtgtgctcac ctaaagctgt ggttttgctg tgttcacttc gatttttctg
     3361 gtctgtggag aaactgtgaa ttggagaaat ggagctctgt ggcttcccac ccaaaccttc
     3421 tcagtccagc tggaggctgg agggagacac aggccccacc cagcagactg aggggcagag
     3481 gcacaggtgg gagggcagcg gagatcagcg tggacaggag cgatgcactt tgtagatgct
     3541 gtggctttgt gttgcgtttt gtgtctctgt tgcacagatc tgttttttca cactgatccg
     3601 tattcccctg ggtgtgcaca cagggcgggt gtggggcatt taggccatgc tgtgctctac
     3661 ttcattgagt aaaatcgagt gagaggttcc gggcagcagg atcgacgccc agtccagccg
     3721 gcagagggaa cacacgggtc cttcattgtc ctgtaagggt gttgaagatg ctccctggcg
     3781 gcccccaagc agactagatg ggaggaggcg ccgctcagcc cctcaccctg catcactgaa
     3841 gagcggcgcc tctgcagcaa gcagggcttc aggaggtgcc cgctggccac agccaggttt
     3901 tccctaagaa gatgttattt tgttgggttt tgttccccct ccatctcgat tctcgtaccc
     3961 aactaaaaaa aaaaaaataa agaaaaaatg tgctgcgttc tgaaaaataa ctccttagct
     4021 tggtctgatt gttttcagac cttaaaatat aaacttgttt cacaagcttt aatccatgtg
     4081 gatttttttt ttcttagaga accacaaaac ataaaaggag caagtcggac tgaatacctg
     4141 tttccatagt gcccacaggg tattcctcac attttctcca tagaagatgc tttttcccaa
     4201 ggctagaacg acttccacca tgatgaattt gctttttagg tcttaattat ttcacttctt
     4261 tttagaaact taggaagaag tggataatcc tgaggtcaca caatctgtcc tcccagaaat
     4321 gaacaaaagt catcaccttt tctgcttgct acacaggcaa cgattccccc atcagctgcc
     4381 cggacccttt ggcctggctt ggtgtgcagg cctgtctgtt tgcttaaagt cagtgggttc
     4441 tggtgcaggg agtgagaagt gggggaagtg aaagggaaag catccgtgag aaagcggcca
     4501 cggttttccc tccttgtgtg cccatggggc accagctcat ggtctttttc agtcatccca
     4561 gtttgtacag acttagcttc tgaactctaa gaatgccaaa gggaccgacg agactcccca
     4621 tcacagcgag ctctgtcctt acatgtattt gatgtgcatc agcggaggag aacactggct
     4681 tggccctgct ccgctgagtg tctgtgaaat acctctactt tccctcccat atccagaaca
     4741 aaatgatact tgacatcctt ccacaaaagt cagcctaaag aagttatggt atcatatgtt
     4801 aaactaagct ttcaaaaacc ttagtgaaat agcaagtgac tgctttcaag cagcagtcga
     4861 catgtaaatg aaggtgttct tagaattcgc attttgccag ctcagcgcac ctccacaacg
     4921 aatgaaatgc tccgtatgat ttgcacaaat gacatagacc tccccaaaag ttaactggct
     4981 ctccttcctc acacagttca tcataaccca accccccacc cccgggtcat gaaaatcaca
     5041 gaacttataa acacattgaa ccctagatct caggcttcct gacctaccgc cagtggcccc
     5101 ttgctggcca ccctataggg tcctccttcc ctggcagccc cccatgtggg agaaatacct
     5161 gattctccca atctgcagtg ggagagcttt gctgaattcc atcccaaagt caaacatggg
     5221 caagaggtga ggatttcact tttaccctca agtccgattt gtctgtgatt ttaaactaac
     5281 tgtgtatgta ttgatgtttg gaagattgtt tgaattttaa agtgataata gtacttaatg
     5341 ttatccagta ttgttcatta aatggtgtta tcctaaagct gcacttggga tttttaccta
     5401 acgctttact gattctctca agcacatggc aaagtttgat ttgcactccg ttcatttctg
     5461 acacgttttg ctgcctccta cctttctaag cgtcatgcaa attcgagaat ggagaaggac
     5521 gctgccggtc cctgagcggt gtggagaggg cggaaggtgg actccagcgc agcttgaggg
     5581 gctgaggacg gaggctgcag catctgtgtc gttctactga gcacgcttct ctgcctcgct
     5641 cctgactcag cactttgttc actggctcag cagttatgtt tacacatcat ttttatgttc
     5701 ctgctttgta attcatgttt gagatgggtg gccactgtac agatatttat tacgctttcc
     5761 agactttctg aatagatttt tttgaataaa catggtttta tgaagtgtaa tctttttcta
     5821 gcctaacaat aa
//
