LOCUS       NM_002954                785 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens ribosomal protein S27a (RPS27A), transcript variant 1,
            mRNA.
ACCESSION   NM_002954
VERSION     NM_002954.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Lee,S.O., Kelliher,J.L., Song,W., Tengler,K., Sarkar,A., Dray,E.
            and Leung,J.W.C.
  TITLE     UBA80 and UBA52 fine-tune RNF168-dependent histone ubiquitination
            and DNA repair
  JOURNAL   J Biol Chem 299 (8), 105043 (2023)
   PUBMED   37451480
  REMARK    GeneRIF: UBA80 and UBA52 fine-tune RNF168-dependent histone
            ubiquitination and DNA repair.
REFERENCE   2  (bases 1 to 785)
  AUTHORS   Eastham,M.J., Pelava,A., Wells,G.R., Watkins,N.J. and Schneider,C.
  TITLE     RPS27a and RPL40, Which Are Produced as Ubiquitin Fusion Proteins,
            Are Not Essential for p53 Signalling
  JOURNAL   Biomolecules 13 (6), 898 (2023)
   PUBMED   37371478
  REMARK    GeneRIF: RPS27a and RPL40, Which Are Produced as Ubiquitin Fusion
            Proteins, Are Not Essential for p53 Signalling.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 785)
  AUTHORS   Luo,J., Zhao,H., Chen,L. and Liu,M.
  TITLE     Multifaceted functions of RPS27a: An unconventional ribosomal
            protein
  JOURNAL   J Cell Physiol 238 (3), 485-497 (2023)
   PUBMED   36580426
  REMARK    GeneRIF: Multifaceted functions of RPS27a: An unconventional
            ribosomal protein.
            Review article
REFERENCE   4  (bases 1 to 785)
  AUTHORS   Singh,S., Vanden Broeck,A., Miller,L., Chaker-Margot,M. and
            Klinge,S.
  TITLE     Nucleolar maturation of the human small subunit processome
  JOURNAL   Science 373 (6560), eabj5338 (2021)
   PUBMED   34516797
REFERENCE   5  (bases 1 to 785)
  AUTHORS   Wang,H., Zhao,J., Yang,J., Wan,S., Fu,Y., Wang,X., Zhou,T.,
            Zhang,Z. and Shen,J.
  TITLE     PICT1 is critical for regulating the Rps27a-Mdm2-p53 pathway by
            microtubule polymerization inhibitor against cervical cancer
  JOURNAL   Biochim Biophys Acta Mol Cell Res 1868 (10), 119084 (2021)
   PUBMED   34166715
  REMARK    GeneRIF: PICT1 is critical for regulating the Rps27a-Mdm2-p53
            pathway by microtubule polymerization inhibitor against cervical
            cancer.
REFERENCE   6  (bases 1 to 785)
  AUTHORS   Adams,S.M., Sharp,M.G., Walker,R.A., Brammar,W.J. and Varley,J.M.
  TITLE     Differential expression of translation-associated genes in benign
            and malignant human breast tumours
  JOURNAL   Br J Cancer 65 (1), 65-71 (1992)
   PUBMED   1370760
REFERENCE   7  (bases 1 to 785)
  AUTHORS   Kanayama,H., Tanaka,K., Aki,M., Kagawa,S., Miyaji,H., Satoh,M.,
            Okada,F., Sato,S., Shimbara,N. and Ichihara,A.
  TITLE     Changes in expressions of proteasome and ubiquitin genes in human
            renal cancer cells
  JOURNAL   Cancer Res 51 (24), 6677-6685 (1991)
   PUBMED   1660345
REFERENCE   8  (bases 1 to 785)
  AUTHORS   Pancre,V., Pierce,R.J., Fournier,F., Mehtali,M., Delanoye,A.,
            Capron,A. and Auriault,C.
  TITLE     Effect of ubiquitin on platelet functions: possible identity with
            platelet activity suppressive lymphokine (PASL)
  JOURNAL   Eur J Immunol 21 (11), 2735-2741 (1991)
   PUBMED   1657614
REFERENCE   9  (bases 1 to 785)
  AUTHORS   Redman,K.L. and Rechsteiner,M.
  TITLE     Identification of the long ubiquitin extension as ribosomal protein
            S27a
  JOURNAL   Nature 338 (6214), 438-440 (1989)
   PUBMED   2538756
REFERENCE   10 (bases 1 to 785)
  AUTHORS   Monia,B.P., Ecker,D.J., Jonnalagadda,S., Marsh,J., Gotlib,L.,
            Butt,T.R. and Crooke,S.T.
  TITLE     Gene synthesis, expression, and processing of human ubiquitin
            carboxyl extension proteins
  JOURNAL   J Biol Chem 264 (7), 4093-4103 (1989)
   PUBMED   2537304
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff in
            collaboration with Francesco Amaldi. The reference sequence was
            derived from AC012358.9.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Aug 7, 2020 this sequence version replaced NM_002954.5.
            
            Summary: Ubiquitin, a highly conserved protein that has a major
            role in targeting cellular proteins for degradation by the 26S
            proteosome, is synthesized as a precursor protein consisting of
            either polyubiquitin chains or a single ubiquitin fused to an
            unrelated protein. This gene encodes a fusion protein consisting of
            ubiquitin at the N terminus and ribosomal protein S27a at the C
            terminus. When expressed in yeast, the protein is
            post-translationally processed, generating free ubiquitin monomer
            and ribosomal protein S27a. Ribosomal protein S27a is a component
            of the 40S subunit of the ribosome and belongs to the S27AE family
            of ribosomal proteins. It contains C4-type zinc finger domains and
            is located in the cytoplasm. Pseudogenes derived from this gene are
            present in the genome. As with ribosomal protein S27a, ribosomal
            protein L40 is also synthesized as a fusion protein with ubiquitin;
            similarly, ribosomal protein S30 is synthesized as a fusion protein
            with the ubiquitin-like protein fubi. Multiple alternatively
            spliced transcript variants that encode the same proteins have been
            identified.[provided by RefSeq, Sep 2008].
            
            Transcript Variant: This variant (1) represents the longest
            transcript. Variants 1, 2 and 3 encode the same protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: CB113428.1, AK225718.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000272317.11/ ENSP00000272317.6
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-21                AC012358.9         37095-37115
            22-86               AC012358.9         37215-37279
            87-141              AC012358.9         37770-37824
            142-227             AC012358.9         38526-38611
            228-359             AC012358.9         39238-39369
            360-785             AC012358.9         39835-40260
FEATURES             Location/Qualifiers
     source          1..785
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2p16.1"
     gene            1..785
                     /gene="RPS27A"
                     /gene_synonym="CEP80; eS31; HEL112; S27A; UBA80; UBC;
                     UBCEP1; UBCEP80"
                     /note="ribosomal protein S27a"
                     /db_xref="GeneID:6233"
                     /db_xref="HGNC:HGNC:10417"
                     /db_xref="MIM:191343"
     exon            1..21
                     /gene="RPS27A"
                     /gene_synonym="CEP80; eS31; HEL112; S27A; UBA80; UBC;
                     UBCEP1; UBCEP80"
                     /inference="alignment:Splign:2.1.0"
     exon            22..86
                     /gene="RPS27A"
                     /gene_synonym="CEP80; eS31; HEL112; S27A; UBA80; UBC;
                     UBCEP1; UBCEP80"
                     /inference="alignment:Splign:2.1.0"
     CDS             39..509
                     /gene="RPS27A"
                     /gene_synonym="CEP80; eS31; HEL112; S27A; UBA80; UBC;
                     UBCEP1; UBCEP80"
                     /note="ubiquitin and ribosomal protein S27a;
                     ubiquitin-CEP80; ubiquitin-40S ribosomal protein S27a;
                     ubiquitin carboxyl extension protein 80; ubiquitin C;
                     epididymis luminal protein 112; ubiquitin-ribosomal
                     protein eS31 fusion protein"
                     /codon_start=1
                     /product="ubiquitin-ribosomal protein eS31 fusion protein
                     precursor"
                     /protein_id="NP_002945.1"
                     /db_xref="CCDS:CCDS33202.1"
                     /db_xref="GeneID:6233"
                     /db_xref="HGNC:HGNC:10417"
                     /db_xref="MIM:191343"
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLI
                     FAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGAKKRKKKSYTTPKKNKHKRKKVKLAV
                     LKYYKVDENGKISRLRRECPSDECGAGVFMASHFDRHYCGKCCLTYCFNKPEDK"
     exon            87..141
                     /gene="RPS27A"
                     /gene_synonym="CEP80; eS31; HEL112; S27A; UBA80; UBC;
                     UBCEP1; UBCEP80"
                     /inference="alignment:Splign:2.1.0"
     exon            142..227
                     /gene="RPS27A"
                     /gene_synonym="CEP80; eS31; HEL112; S27A; UBA80; UBC;
                     UBCEP1; UBCEP80"
                     /inference="alignment:Splign:2.1.0"
     exon            228..359
                     /gene="RPS27A"
                     /gene_synonym="CEP80; eS31; HEL112; S27A; UBA80; UBC;
                     UBCEP1; UBCEP80"
                     /inference="alignment:Splign:2.1.0"
     exon            360..785
                     /gene="RPS27A"
                     /gene_synonym="CEP80; eS31; HEL112; S27A; UBA80; UBC;
                     UBCEP1; UBCEP80"
                     /inference="alignment:Splign:2.1.0"
     regulatory      521..526
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RPS27A"
                     /gene_synonym="CEP80; eS31; HEL112; S27A; UBA80; UBC;
                     UBCEP1; UBCEP80"
                     /note="hexamer: AATAAA"
     polyA_site      541
                     /gene="RPS27A"
                     /gene_synonym="CEP80; eS31; HEL112; S27A; UBA80; UBC;
                     UBCEP1; UBCEP80"
                     /note="major polyA site"
     regulatory      563..568
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RPS27A"
                     /gene_synonym="CEP80; eS31; HEL112; S27A; UBA80; UBC;
                     UBCEP1; UBCEP80"
                     /note="hexamer: AGTAAA"
     polyA_site      591
                     /gene="RPS27A"
                     /gene_synonym="CEP80; eS31; HEL112; S27A; UBA80; UBC;
                     UBCEP1; UBCEP80"
     polyA_site      785
                     /gene="RPS27A"
                     /gene_synonym="CEP80; eS31; HEL112; S27A; UBA80; UBC;
                     UBCEP1; UBCEP80"
ORIGIN      
        1 cttttcgatc cgccatctgc ggtggagccg ccaccaaaat gcagattttc gtgaaaaccc
       61 ttacggggaa gaccatcacc ctcgaggttg aaccctcgga tacgatagaa aatgtaaagg
      121 ccaagatcca ggataaggaa ggaattcctc ctgatcagca gagactgatc tttgctggca
      181 agcagctgga agatggacgt actttgtctg actacaatat tcaaaaggag tctactcttc
      241 atcttgtgtt gagacttcgt ggtggtgcta agaaaaggaa gaagaagtct tacaccactc
      301 ccaagaagaa taagcacaag agaaagaagg ttaagctggc tgtcctgaaa tattataagg
      361 tggatgagaa tggcaaaatt agtcgccttc gtcgagagtg cccttctgat gaatgtggtg
      421 ctggggtgtt tatggcaagt cactttgaca gacattattg tggcaaatgt tgtctgactt
      481 actgtttcaa caaaccagaa gacaagtaac tgtatgagtt aataaaagac atgaactaac
      541 atttattgtt gggttttatt gcagtaaaaa gaatggtttt taagcaccaa attgatggtc
      601 acaccatttc cttttagtag tgctactgct atcgctgtgt gaatgttgcc tctggggatt
      661 atgtgaccca gtggttctgt atacctgcca ggtgccaacc acttgtaaag gtcttgatat
      721 tttcaattct tagactacct atactttggc agaagttata tttaatgtaa gttgtctaaa
      781 tataa
//
