LOCUS       NM_024839                528 bp    mRNA    linear   PRI 09-MAR-2025
DEFINITION  Homo sapiens ribonuclease P subunit p21 (RPP21), transcript variant
            2, mRNA.
ACCESSION   NM_024839
VERSION     NM_024839.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 528)
  AUTHORS   Wu,J., Niu,S., Tan,M., Huang,C., Li,M., Song,Y., Wang,Q., Chen,J.,
            Shi,S., Lan,P. and Lei,M.
  TITLE     Cryo-EM Structure of the Human Ribonuclease P Holoenzyme
  JOURNAL   Cell 175 (5), 1393-1404 (2018)
   PUBMED   30454648
REFERENCE   3  (bases 1 to 528)
  AUTHORS   Abu-Zhayia,E.R., Khoury-Haddad,H., Guttmann-Raviv,N., Serruya,R.,
            Jarrous,N. and Ayoub,N.
  TITLE     A role of human RNase P subunits, Rpp29 and Rpp21, in homology
            directed-repair of double-strand breaks
  JOURNAL   Sci Rep 7 (1), 1002 (2017)
   PUBMED   28432356
  REMARK    GeneRIF: Results demonstrate that Rpp29 and Rpp21 depletion impairs
            double-strand break (DSB) repair by homology-directed repair (HDR),
            but has no deleterious effect on the integrity of non-homologous
            end joining. Rpp29 and Rpp21 are rapidly and transiently recruited
            to laser-microirradiated sites. They bind poly ADP-ribose moieties
            and are recruited to DNA damage sites in a PARP1-dependent manner.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 528)
  AUTHORS   Barcellos,L.F., May,S.L., Ramsay,P.P., Quach,H.L., Lane,J.A.,
            Nititham,J., Noble,J.A., Taylor,K.E., Quach,D.L., Chung,S.A.,
            Kelly,J.A., Moser,K.L., Behrens,T.W., Seldin,M.F., Thomson,G.,
            Harley,J.B., Gaffney,P.M. and Criswell,L.A.
  TITLE     High-density SNP screening of the major histocompatibility complex
            in systemic lupus erythematosus demonstrates strong evidence for
            independent susceptibility regions
  JOURNAL   PLoS Genet 5 (10), e1000696 (2009)
   PUBMED   19851445
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   5  (bases 1 to 528)
  AUTHORS   Han,A., Song,Z., Tong,C., Hu,D., Bi,X., Augenlicht,L.H. and Yang,W.
  TITLE     Sulindac suppresses beta-catenin expression in human cancer cells
  JOURNAL   Eur J Pharmacol 583 (1), 26-31 (2008)
   PUBMED   18291362
REFERENCE   6  (bases 1 to 528)
  AUTHORS   Roberts,J.D. Jr., Chiche,J.D., Kolpa,E.M., Bloch,D.B. and
            Bloch,K.D.
  TITLE     cGMP-dependent protein kinase I interacts with TRIM39R, a novel
            Rpp21 domain-containing TRIM protein
  JOURNAL   Am J Physiol Lung Cell Mol Physiol 293 (4), L903-L912 (2007)
   PUBMED   17601797
REFERENCE   7  (bases 1 to 528)
  AUTHORS   Welting,T.J., Kikkert,B.J., van Venrooij,W.J. and Pruijn,G.J.
  TITLE     Differential association of protein subunits with the human RNase
            MRP and RNase P complexes
  JOURNAL   RNA 12 (7), 1373-1382 (2006)
   PUBMED   16723659
REFERENCE   8  (bases 1 to 528)
  AUTHORS   Welting,T.J., van Venrooij,W.J. and Pruijn,G.J.
  TITLE     Mutual interactions between subunits of the human RNase MRP
            ribonucleoprotein complex
  JOURNAL   Nucleic Acids Res 32 (7), 2138-2146 (2004)
   PUBMED   15096576
  REMARK    Publication Status: Online-Only
REFERENCE   9  (bases 1 to 528)
  AUTHORS   Jarrous,N., Reiner,R., Wesolowski,D., Mann,H., Guerrier-Takada,C.
            and Altman,S.
  TITLE     Function and subnuclear distribution of Rpp21, a protein subunit of
            the human ribonucleoprotein ribonuclease P
  JOURNAL   RNA 7 (8), 1153-1164 (2001)
   PUBMED   11497433
REFERENCE   10 (bases 1 to 528)
  AUTHORS   Jiang,T., Guerrier-Takada,C. and Altman,S.
  TITLE     Protein-RNA interactions in the subunits of human nuclear RNase P
  JOURNAL   RNA 7 (7), 937-941 (2001)
   PUBMED   11455963
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DA270919.1, AJ504713.1 and BG708140.1.
            
            On Feb 27, 2020 this sequence version replaced NM_024839.3.
            
            Summary: RPP21 is a protein subunit of nuclear ribonuclease P,
            which processes the 5-prime leader sequence of precursor tRNAs
            (Jarrous et al., 2001 [PubMed 11497433]).[supplied by OMIM, Jan
            2009].
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice site in the 5' coding region, compared to variant 1,
            resulting in an isoform (2) that is shorter than isoform 1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF212152.2, BC011730.2 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968189
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000442966.7/ ENSP00000403833.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-13                DA270919.1         28-40
            14-527              AJ504713.1         1-514
            528-528             BG708140.1         586-586
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6p22.1"
     gene            1..528
                     /gene="RPP21"
                     /gene_synonym="C6orf135; CAT60"
                     /note="ribonuclease P subunit p21"
                     /db_xref="GeneID:79897"
                     /db_xref="HGNC:HGNC:21300"
                     /db_xref="MIM:612524"
     exon            1..73
                     /gene="RPP21"
                     /gene_synonym="C6orf135; CAT60"
                     /inference="alignment:Splign:2.1.0"
     CDS             17..481
                     /gene="RPP21"
                     /gene_synonym="C6orf135; CAT60"
                     /EC_number="3.1.26.5"
                     /note="isoform 2 is encoded by transcript variant 2;
                     ribonucleoprotein V; ribonuclease P/MRP 21kDa subunit;
                     RNaseP protein p21; ribonuclease P protein subunit p21;
                     ribonuclease P/MRP subunit p21"
                     /codon_start=1
                     /product="ribonuclease P protein subunit p21 isoform 2"
                     /protein_id="NP_079115.1"
                     /db_xref="CCDS:CCDS4679.1"
                     /db_xref="GeneID:79897"
                     /db_xref="HGNC:HGNC:21300"
                     /db_xref="MIM:612524"
                     /translation="MAGPVKDREAFQRLNFLYQAAHCVLAQDPENQALARFYCYTERT
                     IAKRLVLRRDPSVKRTLCRGCSSLLVPGLTCTQRQRRCRGQRWTVQTCLTCQRSQRFL
                     NDPGHLLWGDRPEAQLGSQADSKPLQPLPNTAHSISDRLPEEKMQTQGSSNQ"
     exon            74..174
                     /gene="RPP21"
                     /gene_synonym="C6orf135; CAT60"
                     /inference="alignment:Splign:2.1.0"
     exon            175..257
                     /gene="RPP21"
                     /gene_synonym="C6orf135; CAT60"
                     /inference="alignment:Splign:2.1.0"
     exon            258..383
                     /gene="RPP21"
                     /gene_synonym="C6orf135; CAT60"
                     /inference="alignment:Splign:2.1.0"
     exon            384..528
                     /gene="RPP21"
                     /gene_synonym="C6orf135; CAT60"
                     /inference="alignment:Splign:2.1.0"
     regulatory      502..507
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RPP21"
                     /gene_synonym="C6orf135; CAT60"
                     /note="hexamer: AATAAA"
     polyA_site      528
                     /gene="RPP21"
                     /gene_synonym="C6orf135; CAT60"
                     /note="major polyA site"
ORIGIN      
        1 ctggagcgcg gcggtgatgg cggggccggt gaaggaccgc gaggccttcc agaggctcaa
       61 cttcctgtac caggccgccc attgtgtcct tgcccaggac cccgagaacc aggcgctggc
      121 gaggttttac tgctacactg agaggaccat tgcgaagcgg ctcgtcttgc ggcgggatcc
      181 ctcggtgaag aggactctct gtcgaggctg ctcttccctc ctcgtcccgg gcctcacctg
      241 cacccagcgc cagagacgct gcaggggaca gcgctggacc gtacagacct gcctaacatg
      301 ccagcgcagc caacgcttcc tcaatgatcc cgggcattta ctctggggag acaggcctga
      361 ggcccagctc gggagccaag cagattccaa accactacaa cccttgccaa acacagccca
      421 ctccatttca gaccgccttc ctgaggagaa aatgcagact cagggttcca gtaaccagtg
      481 atggattcac cccatctccc aaataaagtt tacttgtttt acattcca
//
