LOCUS       NM_002951               2227 bp    mRNA    linear   PRI 20-FEB-2025
DEFINITION  Homo sapiens ribophorin II (RPN2), transcript variant 1, mRNA.
ACCESSION   NM_002951
VERSION     NM_002951.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Lampson,B.L., Ramiotarez,A.S., Baro,M., He,L., Hegde,M., Koduri,V.,
            Pfaff,J.L., Hanna,R.E., Kowal,J., Shirole,N.H., He,Y., Doench,J.G.,
            Contessa,J.N., Locher,K.P. and Kaelin,W.G. Jr.
  TITLE     Positive selection CRISPR screens reveal a druggable pocket in an
            oligosaccharyltransferase required for inflammatory signaling to
            NF-kappaB
  JOURNAL   Cell 187 (9), 2209-2223 (2024)
   PUBMED   38670073
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Han,Z., Wang,Y., Han,L. and Yang,C.
  TITLE     RPN2 in cancer: An overview
  JOURNAL   Gene 857, 147168 (2023)
   PUBMED   36621657
  REMARK    GeneRIF: RPN2 in cancer: An overview.
            Review article
REFERENCE   3  (bases 1 to 2227)
  AUTHORS   Gemmer,M., Chaillet,M.L., van Loenhout,J., Cuevas Arenas,R.,
            Vismpas,D., Grollers-Mulderij,M., Koh,F.A., Albanese,P.,
            Scheltema,R.A., Howes,S.C., Kotecha,A., Fedry,J. and Forster,F.
  TITLE     Visualization of translation and protein biogenesis at the ER
            membrane
  JOURNAL   Nature 614 (7946), 160-167 (2023)
   PUBMED   36697828
REFERENCE   4  (bases 1 to 2227)
  AUTHORS   Li,B., Zhao,R., Qiu,W., Pan,Z., Zhao,S., Qi,Y., Qiu,J., Zhang,S.,
            Guo,Q., Fan,Y., Xu,H., Li,M., Li,G. and Xue,H.
  TITLE     The N6-methyladenosine-mediated lncRNA WEE2-AS1 promotes
            glioblastoma progression by stabilizing RPN2
  JOURNAL   Theranostics 12 (14), 6363-6379 (2022)
   PUBMED   36168628
  REMARK    GeneRIF: The N(6)-methyladenosine-mediated lncRNA WEE2-AS1 promotes
            glioblastoma progression by stabilizing RPN2.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2227)
  AUTHORS   Zhou,J., Zhang,J., Zhang,W., Ke,Z., Lv,Y., Zhang,B. and Liao,Z.
  TITLE     Ribophorin II promotes the epithelial-mesenchymal transition and
            aerobic glycolysis of laryngeal squamous cell carcinoma via
            regulating reactive oxygen species-mediated
            Phosphatidylinositol-3-Kinase/Protein Kinase B activation
  JOURNAL   Bioengineered 13 (3), 5141-5151 (2022)
   PUBMED   35156537
  REMARK    GeneRIF: Ribophorin II promotes the epithelial-mesenchymal
            transition and aerobic glycolysis of laryngeal squamous cell
            carcinoma via regulating reactive oxygen species-mediated
            Phosphatidylinositol-3-Kinase/Protein Kinase B activation.
REFERENCE   6  (bases 1 to 2227)
  AUTHORS   Fu,J. and Kreibich,G.
  TITLE     Retention of subunits of the oligosaccharyltransferase complex in
            the endoplasmic reticulum
  JOURNAL   J Biol Chem 275 (6), 3984-3990 (2000)
   PUBMED   10660554
REFERENCE   7  (bases 1 to 2227)
  AUTHORS   Kumar,V., Heinemann,F.S. and Ozols,J.
  TITLE     Interleukin-2 induces N-glycosylation in T-cells: characterization
            of human lymphocyte oligosaccharyltransferase
  JOURNAL   Biochem Biophys Res Commun 247 (2), 524-529 (1998)
   PUBMED   9642163
REFERENCE   8  (bases 1 to 2227)
  AUTHORS   Stoffel,M., Xiang,K. and Bell,G.I.
  TITLE     Dinucleotide repeat polymorphism at the human ribophorin II locus
            (RPN2) on chromosome 20q
  JOURNAL   Hum Mol Genet 1 (8), 656 (1992)
   PUBMED   1301181
REFERENCE   9  (bases 1 to 2227)
  AUTHORS   Loffler,C., Rao,V.V. and Hansmann,I.
  TITLE     Mapping of the ribophorin II (RPN II) gene to human chromosome
            20q12-q13.1 by in-situ hybridization
  JOURNAL   Hum Genet 87 (2), 221-222 (1991)
   PUBMED   2066112
REFERENCE   10 (bases 1 to 2227)
  AUTHORS   Crimaudo,C., Hortsch,M., Gausepohl,H. and Meyer,D.I.
  TITLE     Human ribophorins I and II: the primary structure and membrane
            topology of two highly conserved rough endoplasmic
            reticulum-specific glycoproteins
  JOURNAL   EMBO J 6 (1), 75-82 (1987)
   PUBMED   3034581
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BG718075.1, BC020222.1,
            BF923732.1 and BC002380.2.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_002951.4.
            
            Summary: This gene encodes a type I integral membrane protein found
            only in the rough endoplasmic reticulum. The encoded protein is
            part of an N-oligosaccharyl transferase complex that links high
            mannose oligosaccharides to asparagine residues found in the
            Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This
            protein is similar in sequence to the yeast oligosaccharyl
            transferase subunit SWP1. Alternatively spliced transcript variants
            encoding different isoforms have been found for this gene.
            [provided by RefSeq, Oct 2008].
            
            Transcript Variant: This variant (1) encodes isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: Y00282.1, SRR1660803.237511.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000237530.11/ ENSP00000237530.6
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-41                BG718075.1         290-330
            42-1851             BC020222.1         197-2006
            1852-1910           BF923732.1         207-265
            1911-2227           BC002380.2         1469-1785
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20q11.23"
     gene            1..2227
                     /gene="RPN2"
                     /gene_synonym="RIBIIR; RPN-II; RPNII; SWP1"
                     /note="ribophorin II"
                     /db_xref="GeneID:6185"
                     /db_xref="HGNC:HGNC:10382"
                     /db_xref="MIM:180490"
     exon            1..40
                     /gene="RPN2"
                     /gene_synonym="RIBIIR; RPN-II; RPNII; SWP1"
                     /inference="alignment:Splign:2.1.0"
     CDS             28..1923
                     /gene="RPN2"
                     /gene_synonym="RIBIIR; RPN-II; RPNII; SWP1"
                     /EC_number="2.4.99.18"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; dolichyl-diphosphooligosaccharide--protein
                     glycosyltransferase 63 kDa subunit;
                     dolichyl-diphosphooligosaccharide--protein
                     glycosyltransferase subunit 2; ribophorin-2;
                     oligosaccharyltransferase complex subunit (non-catalytic)"
                     /codon_start=1
                     /product="dolichyl-diphosphooligosaccharide--protein
                     glycosyltransferase subunit 2 isoform 1 precursor"
                     /protein_id="NP_002942.2"
                     /db_xref="CCDS:CCDS13291.1"
                     /db_xref="GeneID:6185"
                     /db_xref="HGNC:HGNC:10382"
                     /db_xref="MIM:180490"
                     /translation="MAPPGSSTVFLLALTIIASTWALTPTHYLTKHDVERLKASLDRP
                     FTNLESAFYSIVGLSSLGAQVPDAKKACTYIRSNLDPSNVDSLFYAAQASQALSGCEI
                     SISNETKDLLLAAVSEDSSVTQIYHAVAALSGFGLPLASQEALSALTARLSKEETVLA
                     TVQALQTASHLSQQADLRSIVEEIEDLVARLDELGGVYLQFEEGLETTALFVAATYKL
                     MDHVGTEPSIKEDQVIQLMNAIFSKKNFESLSEAFSVASAAAVLSHNRYHVPVVVVPE
                     GSASDTHEQAILRLQVTNVLSQPLTQATVKLEHAKSVASRATVLQKTSFTPVGDVFEL
                     NFMNVKFSSGYYDFLVEVEGDNRYIANTVELRVKISTEVGITNVDLSTVDKDQSIAPK
                     TTRVTYPAKAKGTFIADSHQNFALFFQLVDVNTGAELTPHQTFVRLHNQKTGQEVVFV
                     AEPDNKNVYKFELDTSERKIEFDSASGTYTLYLIIGDATLKNPILWNVADVVIKFPEE
                     EAPSTVLSQNLFTPKQEIQHLFREPEKRPPTVVSNTFTALILSPLLLLFALWIRIGAN
                     VSNFTFAPSTIIFHLGHAAMLGLMYVYWTQLNMFQTLKYLAILGSVTFLAGNRMLAQQ
                     AVKRTAH"
     exon            41..234
                     /gene="RPN2"
                     /gene_synonym="RIBIIR; RPN-II; RPNII; SWP1"
                     /inference="alignment:Splign:2.1.0"
     exon            235..330
                     /gene="RPN2"
                     /gene_synonym="RIBIIR; RPN-II; RPNII; SWP1"
                     /inference="alignment:Splign:2.1.0"
     exon            331..506
                     /gene="RPN2"
                     /gene_synonym="RIBIIR; RPN-II; RPNII; SWP1"
                     /inference="alignment:Splign:2.1.0"
     exon            507..582
                     /gene="RPN2"
                     /gene_synonym="RIBIIR; RPN-II; RPNII; SWP1"
                     /inference="alignment:Splign:2.1.0"
     exon            583..717
                     /gene="RPN2"
                     /gene_synonym="RIBIIR; RPN-II; RPNII; SWP1"
                     /inference="alignment:Splign:2.1.0"
     exon            718..894
                     /gene="RPN2"
                     /gene_synonym="RIBIIR; RPN-II; RPNII; SWP1"
                     /inference="alignment:Splign:2.1.0"
     exon            895..1013
                     /gene="RPN2"
                     /gene_synonym="RIBIIR; RPN-II; RPNII; SWP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1014..1119
                     /gene="RPN2"
                     /gene_synonym="RIBIIR; RPN-II; RPNII; SWP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1120..1211
                     /gene="RPN2"
                     /gene_synonym="RIBIIR; RPN-II; RPNII; SWP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1212..1326
                     /gene="RPN2"
                     /gene_synonym="RIBIIR; RPN-II; RPNII; SWP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1327..1521
                     /gene="RPN2"
                     /gene_synonym="RIBIIR; RPN-II; RPNII; SWP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1522..1608
                     /gene="RPN2"
                     /gene_synonym="RIBIIR; RPN-II; RPNII; SWP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1609..1704
                     /gene="RPN2"
                     /gene_synonym="RIBIIR; RPN-II; RPNII; SWP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1705..1780
                     /gene="RPN2"
                     /gene_synonym="RIBIIR; RPN-II; RPNII; SWP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1781..1910
                     /gene="RPN2"
                     /gene_synonym="RIBIIR; RPN-II; RPNII; SWP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1911..2227
                     /gene="RPN2"
                     /gene_synonym="RIBIIR; RPN-II; RPNII; SWP1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2205..2210
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RPN2"
                     /gene_synonym="RIBIIR; RPN-II; RPNII; SWP1"
                     /note="hexamer: AATAAA"
     polyA_site      2227
                     /gene="RPN2"
                     /gene_synonym="RIBIIR; RPN-II; RPNII; SWP1"
                     /note="major polyA site"
ORIGIN      
        1 ggactccggc gggacctgct cggaggaatg gcgccgccgg gttcaagcac tgtcttcctg
       61 ttggccctga caatcatagc cagcacctgg gctctgacgc ccactcacta cctcaccaag
      121 catgacgtgg agagactaaa agcctcgctg gatcgccctt tcacaaattt ggaatctgcc
      181 ttctactcca tcgtgggact cagcagcctt ggtgctcagg tgccagatgc aaagaaagca
      241 tgtacctaca tcagatctaa ccttgatccc agcaatgtgg attccctctt ctacgctgcc
      301 caggccagcc aggccctctc aggatgtgag atctctattt caaatgagac caaagatctg
      361 cttctggcag ctgtcagtga ggactcatct gttacccaga tctaccatgc agttgcagct
      421 ctaagtggct ttggccttcc cttggcatcc caagaagcac tcagtgccct tactgctcgt
      481 ctcagcaagg aggagactgt gctggcaaca gtccaggctc tgcagacagc atcccacctg
      541 tcccagcagg ctgacctgag gagcatcgtg gaggagattg aggaccttgt tgctcgcctg
      601 gatgaactcg ggggcgtgta tctccagttt gaagaaggac tggaaacaac agcgttattt
      661 gtggctgcca cctacaagct catggatcat gtggggactg agccatccat taaggaggat
      721 caggtcatcc agctgatgaa cgcgatcttc agcaagaaga actttgagtc cctctccgaa
      781 gccttcagcg tggcctctgc agctgctgtg ctctcgcata atcgctacca cgtgccagtt
      841 gtggttgtgc ctgagggctc tgcttccgac actcatgaac aggctatctt gcggttgcaa
      901 gtcaccaatg ttctgtctca gcctctgact caggccactg ttaaactaga acatgctaaa
      961 tctgttgctt ccagagccac tgtcctccag aagacatcct tcacccctgt aggggatgtt
     1021 tttgaactaa atttcatgaa cgtcaaattt tccagtggtt attatgactt ccttgtcgaa
     1081 gttgaaggtg acaaccggta tattgcaaat accgtagagc tcagagtcaa gatctccact
     1141 gaagttggca tcacaaatgt tgatctttcc accgtggata aggatcagag cattgcaccc
     1201 aaaactaccc gggtgacata cccagccaaa gccaagggca cattcatcgc agacagccac
     1261 cagaacttcg ccttgttctt ccagctggta gatgtgaaca ctggtgctga actcactcct
     1321 caccagacat ttgtccgact ccataaccag aagactggcc aggaagtggt gtttgttgcc
     1381 gagccagaca acaagaacgt gtacaagttt gaactggata cctctgaaag aaagattgaa
     1441 tttgactctg cctctggcac ctacactctc tacttaatca ttggagatgc cactttgaag
     1501 aacccaatcc tctggaatgt ggctgatgtg gtcatcaagt tccctgagga agaagctccc
     1561 tcgactgtct tgtcccagaa ccttttcact ccaaaacagg aaattcagca cctgttccgc
     1621 gagcctgaga agaggccccc caccgtggtg tccaatacat tcactgccct gatcctctcg
     1681 ccgttgcttc tgctcttcgc tctgtggatc cggattggtg ccaatgtctc caacttcact
     1741 tttgctccta gcacgattat atttcacctg ggacatgctg ctatgctggg actcatgtat
     1801 gtctactgga ctcagctcaa catgttccag accttgaagt acctggccat cctgggcagt
     1861 gtgacgtttc tggctggcaa tcggatgctg gcccagcagg cagtcaagag aacagcacat
     1921 tagttccaga agaaagatgg aaattctgaa aactgaatgt caagaaaagg agtcaagaac
     1981 aattcacagt atgagaagaa aaatggaaaa aaaaaacttt atttaaaaaa gaaaaaagtc
     2041 cagattgtag ttatactttt gcttgttttt cagtttcccc aacacacagc agatacctgg
     2101 tgagctcaga tagtctcttt ctctgacact gtgtaagaag ctgtgaatat tcctaactta
     2161 cccagatgtt gcttttgaaa agttgaaatg tgtaattgtt ttggaataaa gagggtaaca
     2221 ataggaa
//
